CN115068352B - Oil control polypeptide flexible liposome hydrogel and preparation method and application thereof - Google Patents
Oil control polypeptide flexible liposome hydrogel and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses an oil control polypeptide flexible liposome hydrogel and a preparation method and application thereof, wherein the hydrogel comprises oil control polypeptide, lecithin, cholesterol, a surfactant, a gel matrix and auxiliary materials, wherein the molar ratio of the lecithin to the cholesterol is 1-12:1, a step of; the mole ratio of lecithin to surfactant is 1-12:1, a step of; the molar ratio of lecithin to oil control polypeptide is 100-400:1, a step of; the total weight of the oil control polypeptide, lecithin, cholesterol and surfactant is 3-6%, the gel matrix is 0.6-20%, the auxiliary material is 4.1-8.3%, and the rest is ultrapure water. The method comprises the following steps: step one, preparing a transparent membranous lipid film; step two, extruding the treated flexible liposome through a membrane with the aperture of 450nm for a plurality of times, and extruding the treated flexible liposome through a membrane with the aperture of 220nm for a plurality of times; and step three, stirring until the mixture is uniform. The application of hydrogel in preparing skin care product with the effect of enhancing the oil control of water-soluble polypeptide. The beneficial effects are that: the percutaneous transmittance of the medicine is improved. Increasing the percutaneous absorption effect; the stability of the liposome is improved.
Description
Technical Field
The invention relates to a hydrogel and a preparation method and application thereof, in particular to an oil-control polypeptide flexible liposome hydrogel and a preparation method and application thereof.
Background
Currently, with respect to the vigorous sebum secretion, astringents are used to shrink pores and close the openings of sebaceous glands, thereby temporarily reducing sebum secretion. However, sebum synthesized in the skin is not reduced, but accumulated in pores, so that the sebum secretion amount is larger after the skin is lighter, and bacterial infection and inflammation are caused by the skin is heavier. Alkaline detergents may also be used to remove the skin fat, however, the skin is extremely dry after use and instead stimulates sebum secretion, with more oily nature. In addition, the sebum can be removed by chemical synthesis or natural mineral components by adsorption, so that the secretion of the grease can be controlled, and after the grease on the skin surface is adsorbed, the secretion of the sebum can be accelerated, so that the adverse effect is caused.
Most numerical control oil products and main preparation methods at present are to mix active ingredients with oil control effect and other components to prepare a composition, for example: a composition containing polysaccharide for keeping moisture and controlling oil, a pure plant essence cosmetic for controlling oil and preventing acne, a cosmetic containing the composition with antiinflammatory and oil controlling effects, etc. Wherein the active ingredients mainly comprise plant extracts, fermentation products, polysaccharides, chemical synthesis or natural mineral components and the like. The product is in the form of cosmetic water, emulsion, skin caring cream, etc. Generally, the composition has the functions of resisting bacteria and diminishing inflammation, astringing pores, absorbing sebum and the like.
According to the prior literature and patent search, no product is studied for molecular mechanism of sebum production at cell level, and a targeted active ingredient is selected for preparation of the product. In addition, the physicochemical properties of the different active ingredients are different, and it is known that some water-soluble ingredients are difficult to penetrate the stratum corneum, so that it is important to prepare a suitable carrier for delivering the active ingredients to the deep skin.
Disclosure of Invention
The main purpose of the invention is to prepare the oil control polypeptide aiming at the sebum production at the cellular level into a novel preparation, thereby achieving the purposes of promoting transdermal absorption, enhancing the oil control effect and improving the stability of the oil control polypeptide;
the other purpose of the invention is to make the lipid membrane of the oil control polypeptide have high deformability, improve the transdermal permeability of the lipid membrane, and improve the encapsulation rate of the oil control polypeptide to more than 90% through prescription optimization;
a further object of the present invention is to improve the stability problems of aggregation, sedimentation, etc. of liposome solutions during storage by means of hydrogel matrices; the retention time of the medicine on the skin surface is prolonged to achieve the slow release effect, and the permeation quantity of the medicine in the skin is improved.
The invention provides an oil control polypeptide flexible liposome hydrogel for solving the problems and achieving the purposes, and a preparation method and application thereof.
The oil control polypeptide flexible liposome hydrogel provided by the invention comprises oil control polypeptide, lecithin, cholesterol, a surfactant, a gel matrix and auxiliary materials, wherein the molar ratio of the lecithin to the cholesterol is 1-12:1, a step of; the mole ratio of lecithin to surfactant is 1-12:1, a step of; the molar ratio of lecithin to oil control polypeptide is 100-400:1, a step of; the total weight of the oil control polypeptide, lecithin, cholesterol and surfactant is 3-6%, the gel matrix is 0.6-20%, the auxiliary material is 4.1-8.3%, and the rest is ultrapure water.
The lecithin is one or more of egg yolk lecithin, soybean lecithin and hydrogenated soybean lecithin, and the surfactant is one or more of sodium deoxycholate, tween-80 and tween-20.
The oil control polypeptide is an active ingredient produced by Jilin province blue Pu Hao industry, science and technology limited company, and is a patent product.
The gel matrix is any one of carbomer, sodium alginate and poloxamer, the auxiliary materials comprise humectant and preservative, the humectant and the preservative account for 4-7% and 0.1-1% of the total mass of the hydrogel respectively, and the humectant is one or a combination of more of hyaluronic acid, glycerol and ceramide; the preservative is one or more of methylparaben, ethylparaben and methylparaben, and when the gel matrix is carbomer and sodium alginate, the auxiliary material is added with a neutralizing agent which accounts for 0-0.3% of the total mass of the hydrogel, and the neutralizing agent is one or more of triethanolamine, diethanolamine, ethylenediamine and calcium chloride.
The invention provides a preparation method of oil-control polypeptide flexible liposome hydrogel, which comprises the following steps:
dissolving lecithin, a surfactant and cholesterol in methanol, and placing the mixture on a rotary evaporator for rotary evaporation at 30-50 ℃ for 1-3 hours to prepare a transparent membranous lipid membrane, wherein the lecithin is one or more of soybean lecithin, hydrogenated soybean lecithin and egg yolk lecithin, the surfactant is one or more of anionic surfactant sodium deoxycholate, nonionic surfactant tween-80 and nonionic surfactant tween-20, and the molar ratio of the lecithin to the cholesterol is 1-12:1, the mole ratio of lecithin to surfactant is 1-12:1, a step of;
step two, adding an oil control polypeptide aqueous solution, wherein the molar ratio of lecithin to the oil control polypeptide aqueous solution is 100-400:1, placing on a shaking table for hydration for 1-4 hours at 30-50 ℃, then carrying out ultrasonic treatment for 10-20 minutes at 100W, extruding the treated flexible liposome through a membrane with the aperture of 450nm for a plurality of times, and extruding through a membrane with the aperture of 220nm for a plurality of times;
stirring and dispersing a gel matrix accounting for 0.6-20% of the total mass in ultrapure water, wherein the gel matrix is any one of carbomer, sodium alginate and poloxamer, then sequentially adding a humectant accounting for 4-7% of the total mass, a preservative accounting for 0.1-1% of the total mass and a liposome solution accounting for 30-60% of the total mass prepared in the step two, wherein the sum of oil control polypeptide, lecithin, cholesterol and surfactant in the liposome solution accounts for 3-6% of the total mass, the rest is ultrapure water, stirring for 20-30 minutes, and when the carbomer and the sodium alginate are selected as the gel matrix, adding a neutralizing agent into the auxiliary materials, wherein the neutralizing agent accounts for 0-0.3% of the total mass of the hydrogel, and stirring until uniformity is achieved.
The invention provides application of oil control polypeptide flexible liposome hydrogel in preparing skin care products with the effect of enhancing water-soluble polypeptide oil control.
The invention has the beneficial effects that:
according to the oil-control polypeptide flexible liposome hydrogel, the preparation method and the application thereof, the common liposome is optimized, the surfactant is introduced to increase the flexibility of a liposome membrane, the liposome component has better biocompatibility with human skin, and the percutaneous transmittance of the medicine is improved. Comparing the encapsulation efficiency of the anionic surfactant sodium deoxycholate and the nonionic surfactant tween-80, the result shows that: the Zeta potential of the obtained flexible liposome is-18.52+/-0.53 mV, the particle size is 80-120nm, and the encapsulation rate of the oil control peptide is 92.46 +/-1.21%. The encapsulation rate of the oil control peptide with positive single charge and the deoxycholate sodium with negative charge reaches more than 90 percent under the action of electrostatic attraction. The surfactant increases the fluidity of liposome membranes, reduces the particle size of the liposome and increases the percutaneous absorption effect; cholesterol can increase the rigidity of the liposome membrane, thereby increasing the stability of the liposome.
The hydrogel provided by the invention optimizes the proportion of the components to ensure that the texture is uniform and the liposome is uniformly dispersed in the gel. Hydrogels can prevent rapid clearance of liposomes from skin sites and can maintain liposome membrane integrity. In addition, the liposome is doped into the hydrogel to endow the liposome to be used locally with enough viscosity, so that the stability problems of aggregation, sedimentation and the like of liposome solution are improved easily in the storage process, the residence time of the drug on the skin surface is prolonged, the permeation quantity of the drug in the skin is improved, and the liposome has good permeation and residence capacity compared with an oil control peptide aqueous solution in a skin permeation and residence experiment. The oil control effect of the flexible liposome hydrogel preparation is verified in a golden-yellow mice hypersebony model and a human body test. The human skin patch test shows the safety of the oil control peptide flexible liposome hydrogel preparation. The preparation provided by the invention has important theoretical value and good application prospect.
Drawings
FIG. 1 is a schematic view showing the appearance of comparative examples SREi-CL, comparative examples SREi-NDL and examples SREi-ADL according to the present invention.
FIG. 2 is a schematic diagram showing the particle size distribution of SREi-ADL according to the embodiment of the present invention.
FIG. 3 is a schematic diagram of a transmission electron microscope of an embodiment SREi-ADL according to the present invention.
FIG. 4 is a schematic diagram of the cumulative skin permeation curves of examples SREi-ADL, SREi-ADL-CAR and comparative examples Free SREi, SREi-ADL-SA, SREi-ADL-PLU according to the present invention.
FIG. 5 is a schematic diagram of a Scanning Electron Microscope (SEM) morphology of an embodiment SREi-ADL-CAR according to the present invention.
FIG. 6 is a schematic view of the appearance of an embodiment SREi-ADL, SREi-ADL-CAR according to the present invention.
FIG. 7 is a schematic representation of the in vitro release profile of SREi-ADL, SREi-ADL-CAR versus Free SREi for the examples of the present invention.
FIG. 8 is a graph showing the change of drug content at 4℃and room temperature for 12 months in examples SREi-ADL, SREi-ADL-CAR and comparative example Free SREi according to the present invention.
FIG. 9 is a schematic representation of the growth curves of the sebaceous glands on the back of golden mice of the blank, examples SREi-ADL, SREi-ADL-CAR and comparative examples Free SREi, ADL-CAR according to the invention.
FIG. 10 is a schematic representation of the staining of a golden yellow mice back sebaceous paraffin section HE according to the invention, A being blank, B being comparative example ADL-CAR, C being comparative example Free SREi, D being example SREi-ADL, E being example SREi-ADL-CAR.
Detailed Description
Please refer to fig. 1 to 10:
embodiment one:
the oil control polypeptide flexible liposome hydrogel provided by the invention comprises oil control polypeptide, lecithin, cholesterol, a surfactant, a gel matrix and auxiliary materials, wherein the molar ratio of the lecithin to the cholesterol is 1:1, a step of; the molar ratio of lecithin to surfactant is 6:1, a step of; the molar ratio of lecithin to oil control polypeptide is 100:1, a step of; the total mass of the oil control polypeptide, lecithin, cholesterol and surfactant is 3%, the gel matrix is 20%, the auxiliary material is 8% of the total mass, and the rest is ultrapure water.
The lecithin is one or more of egg yolk lecithin, soybean lecithin and hydrogenated soybean lecithin, and the surfactant is one or more of sodium deoxycholate, tween-80 and tween-20.
The oil control polypeptide is an active ingredient produced by Jilin province blue Pu Hao industry, science and technology limited company, and is a patent product.
The gel matrix is poloxamer, the auxiliary materials comprise humectant and preservative, the humectant and the preservative account for 7% and 1% of the total mass of the hydrogel respectively, and the humectant is one or more of hyaluronic acid, glycerol and ceramide; the antiseptic is one or more of methylparaben, ethylparaben and methylparaben.
The invention provides a preparation method of oil-control polypeptide flexible liposome hydrogel, which comprises the following steps:
dissolving lecithin, a surfactant and cholesterol in methanol, and placing the mixture on a rotary evaporator for rotary evaporation at 30-50 ℃ for 1-3 hours to prepare a transparent membranous lipid membrane, wherein the lecithin is one or more of soybean lecithin, hydrogenated soybean lecithin and egg yolk lecithin, the surfactant is one or more of anionic surfactant sodium deoxycholate, nonionic surfactant tween-80 and nonionic surfactant tween-20, and the molar ratio of the lecithin to the cholesterol is 1:1, the molar ratio of lecithin to surfactant is 6:1, a step of;
step two, adding an oil control polypeptide aqueous solution, wherein the molar ratio of lecithin to the oil control polypeptide aqueous solution is 100:1, placing on a shaking table for hydration for 1-4 hours at 30-50 ℃, then carrying out ultrasonic treatment for 10-20 minutes at 100W, extruding the treated flexible liposome through a membrane with the aperture of 450nm for a plurality of times, and extruding through a membrane with the aperture of 220nm for a plurality of times;
stirring and dispersing a gel matrix accounting for 20% of the total mass in ultrapure water, wherein the gel matrix is poloxamer, then sequentially adding a humectant accounting for 7% of the total mass, a preservative accounting for 1% of the total mass and a liposome solution accounting for 30% of the total mass, wherein the total of oil control polypeptide, lecithin, cholesterol and surfactant in the liposome solution accounts for 3% of the total mass, and the balance of the ultrapure water, and stirring for 20-30 minutes until the total mass is uniform.
The invention provides application of oil control polypeptide flexible liposome hydrogel in preparing skin care products with the effect of enhancing water-soluble polypeptide oil control.
Embodiment two:
the oil control polypeptide flexible liposome hydrogel provided by the invention comprises oil control polypeptide, lecithin, cholesterol, a surfactant, a gel matrix and auxiliary materials, wherein the molar ratio of the lecithin to the cholesterol is 6:1, a step of; the molar ratio of lecithin to surfactant is 12:1, a step of; the molar ratio of lecithin to oil control polypeptide is 300:1, a step of; the sum of the oil control polypeptide, lecithin, cholesterol and surfactant accounts for 6 percent of the total mass, the gel matrix accounts for 0.6 percent of the total mass, the auxiliary materials account for 5.1 percent of the total mass, and the balance is ultrapure water.
The lecithin is one or more of egg yolk lecithin, soybean lecithin and hydrogenated soybean lecithin, and the surfactant is one or more of sodium deoxycholate, tween-80 and tween-20.
The oil control polypeptide is an active ingredient produced by Jilin province blue Pu Hao industry, science and technology limited company, and is a patent product.
The gel matrix is any one of carbomer and sodium alginate, the auxiliary materials comprise humectant, neutralizer and preservative, the humectant, the neutralizer and the preservative account for 4 percent, 0.1 percent and 1 percent of the total mass of the hydrogel respectively, and the humectant is one or more of hyaluronic acid, glycerol and ceramide; the neutralizing agent is one or more of triethanolamine, diethanolamine, ethylenediamine and calcium chloride; the antiseptic is one or more of methylparaben, ethylparaben and methylparaben.
The invention provides a preparation method of oil-control polypeptide flexible liposome hydrogel, which comprises the following steps:
dissolving lecithin, a surfactant and cholesterol in methanol, and placing the mixture on a rotary evaporator for rotary evaporation at 30-50 ℃ for 1-3 hours to prepare a transparent membranous lipid membrane, wherein the lecithin is one or more of soybean lecithin, hydrogenated soybean lecithin and egg yolk lecithin, the surfactant is one or more of anionic surfactant sodium deoxycholate, nonionic surfactant tween-80 and nonionic surfactant tween-20, and the molar ratio of the lecithin to the cholesterol is 6:1, the mole ratio of lecithin to surfactant is 12:1, a step of;
step two, adding an oil control polypeptide aqueous solution, wherein the molar ratio of lecithin to the oil control polypeptide aqueous solution is 300:1, placing on a shaking table for hydration for 1-4 hours at 30-50 ℃, then carrying out ultrasonic treatment for 10-20 minutes at 100W, extruding the treated flexible liposome through a membrane with the aperture of 450nm for a plurality of times, and extruding through a membrane with the aperture of 220nm for a plurality of times;
stirring and dispersing a gel matrix accounting for 0.6% of the total mass in ultrapure water, wherein the gel matrix is any one of carbomer and sodium alginate, sequentially adding a humectant accounting for 4% of the total mass, a preservative accounting for 1% of the total mass and a liposome solution accounting for 60% of the total mass prepared in the step two, wherein the sum of oil control polypeptide, lecithin, cholesterol and surfactant in the liposome solution accounts for 6% of the total mass, and the balance is an aqueous solution, stirring for 20-30 minutes, and finally adding a neutralizing agent accounting for 0.1% of the total mass, and stirring until uniformity is achieved.
The invention provides application of oil control polypeptide flexible liposome hydrogel in preparing skin care products with the effect of enhancing water-soluble polypeptide oil control.
Embodiment III:
the oil control polypeptide flexible liposome hydrogel provided by the invention comprises oil control polypeptide, lecithin, cholesterol, a surfactant, a gel matrix and auxiliary materials, wherein the molar ratio of the lecithin to the cholesterol is 12:1, a step of; the molar ratio of lecithin to surfactant is 1:1, a step of; the molar ratio of lecithin to oil control polypeptide is 400:1, a step of; the total weight of the oil control polypeptide, lecithin, cholesterol and surfactant is 4.5%, the gel matrix is 11%, the auxiliary material is 6.0%, and the rest is ultrapure water.
The lecithin is one or more of egg yolk lecithin, soybean lecithin and hydrogenated soybean lecithin, and the surfactant is one or more of sodium deoxycholate, tween-80 and tween-20.
The oil control polypeptide is an active ingredient produced by Jilin province blue Pu Hao industry, science and technology limited company, and is a patent product.
The gel matrix is poloxamer, the auxiliary materials comprise humectant and preservative, the humectant and the preservative account for 5.5% and 0.5% of the total mass of the hydrogel respectively, and the humectant is one or a combination of more of hyaluronic acid, glycerol and ceramide; the antiseptic is one or more of methylparaben, ethylparaben and methylparaben.
The invention provides a preparation method of oil-control polypeptide flexible liposome hydrogel, which comprises the following steps:
dissolving lecithin, a surfactant and cholesterol in methanol, and placing the mixture on a rotary evaporator for rotary evaporation at 30-50 ℃ for 1-3 hours to prepare a transparent membranous lipid membrane, wherein the lecithin is one or more of soybean lecithin, hydrogenated soybean lecithin and egg yolk lecithin, the surfactant is one or more of anionic surfactant sodium deoxycholate, nonionic surfactant tween-80 and nonionic surfactant tween-20, and the molar ratio of the lecithin to the cholesterol is 12:1, the mole ratio of lecithin to surfactant is 1:1, a step of;
step two, adding an oil control polypeptide aqueous solution, wherein the molar ratio of lecithin to the oil control polypeptide aqueous solution is 400:1, placing on a shaking table for hydration for 1-4 hours at 30-50 ℃, then carrying out ultrasonic treatment for 10-20 minutes at 100W, extruding the treated flexible liposome through a membrane with the aperture of 450nm for a plurality of times, and extruding through a membrane with the aperture of 220nm for a plurality of times;
stirring and dispersing 11% of gel matrix in ultrapure water, wherein the gel matrix is poloxamer, then sequentially adding humectant accounting for 5.5% of the total mass, preservative accounting for 0.5% of the total mass and liposome solution accounting for 45% of the total mass, wherein the total of oil control polypeptide, lecithin, cholesterol and surfactant in the liposome accounts for 4.5% of the total mass, and the balance of the ultrapure water, and stirring for 20-30 minutes until the total mass is uniform.
The invention provides application of oil control polypeptide flexible liposome hydrogel in preparing skin care products with the effect of enhancing water-soluble polypeptide oil control.
Examples and comparative examples of specific verification of the present invention are described below:
1. the settings of the examples and comparative examples are specifically as follows:
table 1 abbreviations for examples and comparative examples of the invention
2. Measurement of Liposome encapsulation Rate and appearance observation
The liposomes were demulsified and the encapsulation efficiency (EE%) and drug loading (DL%) were measured by HPLC, and the results are shown in table 2, and the appearance of each liposome is shown in fig. 1.
Table 2 encapsulation efficiency (n=3) of the liposomes of comparative examples 1-2 and example 1 according to the invention
3. Determination of Flexible Liposome particle size, PDI and Zeta potential
The particle size, potential and PDI values (Polydispersity Index, polydispersity) were measured at 25℃using a Markov particle size analyzer from the diluted liposome solution, and the results are shown in Table 3, and the particle size distribution of the optimal flexible liposome formulation SREi-ADL is shown in FIG. 2.
Table 3 particle diameters and potentials (n=3) of the liposomes of comparative examples 1 to 2 and example 1 according to the present invention
4. Appearance of flexible liposome and morphological observation of electron microscope
The optimal flexible liposome formulation was selected as SREi-ADL according to the above results, and a JEM-2100F-type transmission electron microscopy observation of the flexible liposome sample was performed, and the results are shown in FIG. 3.
5. Skin penetration study
Healthy SD rats were taken and the abdominal skin of the rats was used for in vitro transdermal penetration testing. Mechanically removing abdominal skin hair with scissors, and then dehairingThe cream removes the remaining hair, and the hair removal process requires care to keep the integrity of the skin intact. After the rat was sacrificed, the abdominal skin was separated by surgical scissors, subcutaneous fat and adherent tissues were carefully removed, and washed with physiological saline. Preparing each preparation, performing transdermal experiments on the sample to be tested according to the use instructions of a transdermal test diffusion instrument, and harvesting the sample in the receiving pool at 0h, 1h, 2h, 4h, 8h and 12h of the experiment. HPLC detection: ultraviolet detection wavelength: 220nm; mobile phase: 50% solution A: 99.9% H 2 O+0.1% tfa;50% of solution B: 60% ACN+40% H 2 0. The results are shown in Table 4 and FIG. 4.
TABLE 4 cumulative permeation of various oil-control peptide formulations
6. Morphology observation of Flexible Liposome hydrogels
The optimal flexible liposome hydrogel preparation is selected as SREi-ADL-CAR according to the result, the pictures of the flexible liposome SREi-ADL and the prepared gel SREi-ADL-CAR are shown in figure 5, and the SREi-ADL-CAR is freeze-dried for 48 hours. The samples were mounted on metal stubs with double-sided tape and sputter coated with gold under an argon atmosphere before being observed using the dc sputtering technique. The morphology was photographed using a tungsten wire scanning electron microscope (JSM-IT 500A). The results are shown in FIG. 6.
7. Stability investigation
The concentrations of SREi in the preparations were measured at 3 parts at different time points (0 month, 1 month, 2 months, 3 months, 6 months, 9 months, 12 months) at 4℃and room temperature, respectively, and recorded as Cn (n=0, 1, 2, 3, 6, 9, 12), and the percentage of the time drug content was plotted as shown in FIG. 7.
8. In vitro Release Rate investigation
The samples SREi-ADL, SREi-ADL-CAR and comparative example Free SREi were placed in dialysis bags for dialysis at 36℃and appropriate amounts were taken out at 0, 1, 2, 4, 8, 12, 24 and 48 hours, respectively, the concentrations of the oil-controlling peptides were measured and recorded as Cn (n is different time points), the release rates were plotted on the ordinate and the release rate was plotted on the ordinate. The release rate is calculated by the following steps: release rate= (1-Cn/C0) ×100% and the results are shown in table 5 and fig. 8.
Table 5 percent in vitro release of controlled oil peptides from each formulation
9. Animal oil control experiment
Examples SREi-ADL and SREi-ADL-CAR, and comparative examples Free SREi and ADL-CAR were prepared and saline was administered to the blank. 50 golden mice were randomly divided into 5 groups of 10 mice each. The solutions are respectively smeared on sebaceous gland spots for 21 days. The maximum transverse Diameter (DT) and the maximum longitudinal Diameter (DL) of the sebaceous gland spots of the back are measured daily by a vernier caliper, the increase rate of the sebaceous gland size of the golden-yellow mice is calculated as shown in figure 9, the sebaceous gland tissues are taken after the experiment is finished to prepare paraffin sections, and the microstructure of the sebaceous glands of the back is observed by HE staining as shown in figure 10.
10. Patch test
The oil control peptide formulations of examples SREi-ADL and SREi-ADL-CAR were prepared. 24 persons meeting the volunteer inclusion criteria of the subject (the same batch of subjects was used for each test sample) were selected. And (3) adopting a closed test, placing 0.02g of a test object into a patch device, applying the test object onto the bent side of the arm of a test object by using a hypoallergenic adhesive tape, straightening the arm of the test object when the test object is applied, applying the test object from the lower part to the upper part, lightly pressing the adhesive tape by using a palm to discharge air, and applying the test object to the skin for 24 hours, wherein a blank control group is a test sample which is not used. Observation results: after 48 hours from removal of the patch device, the patch device was subjected to interpretation according to the following interpretation criteria, and the results of the statistical tests were shown in Table 6.
TABLE 6 Patch test results
Interpretation criteria: (one) negative reaction; suspicious reaction: only mild erythema; (+) weak positive: erythema, infiltration, and possibly small papules; (++) strong positive: erythema, infiltration, papules, blisters; (+ ++ + electrode) strong positive: erythema, infiltration, papules, blisters and bullae.
11. Skin grease removal experiment
Each formulation of examples SREi-ADL and SREi-ADL-CAR, as well as comparative example Free SREi and comparative example ADL-CAR, was prepared. 48 oily skin volunteers were characterized by obvious pores, high sebum secretion, and visible oily light in the face and T regions. The animals were randomized into 4 groups of 12 persons each. The left and right sides of the face were used as test areas in the experiment. The subjects or caregivers are regulated to adhere to the test sample for use in the morning and evening every day, and the test sample is used for half faces, and the half faces are only wiped by clean water and are continuously used for 14 days. Follow-up was performed at different time periods from the beginning of the experiment, and the skin oil control changes with the product and with clear water were tested separately, and the test of the same volunteer was performed by the same measurer. The results are shown in Table 7:
TABLE 7 results of grease removal test
Test standard: 1) Determination of skin oil content: the fixed parts of the cheeks and forehead of the subjects are used as test areas, the skin grease content of each subject before use and the grease content of the skin on the 14 th day after using the sample are measured by adopting a CBS skin analysis system in an environment of 25 ℃, and the grease clearance rate is calculated according to the following formula and is calculated as an arithmetic average value.
Skin oil clearance = (skin oil content before use-skin oil content after use)/skin oil content before use x 100%.
Claims (2)
1. An oil control polypeptide flexible liposome hydrogel, which is characterized in that: comprises oil control polypeptide, lecithin, cholesterol, a surfactant, a gel matrix and auxiliary materials, wherein the molar ratio of the lecithin to the cholesterol is 6:1, a step of; the molar ratio of lecithin to surfactant is 12:1, a step of; the molar ratio of lecithin to oil control polypeptide is 300:1, a step of; the total mass of the oil control polypeptide, lecithin, cholesterol and surfactant is 6%, the gel matrix is 0.6%, the auxiliary material is 5.1%, the rest is ultrapure water, the lecithin is one or more of egg yolk lecithin, soybean lecithin and hydrogenated soybean lecithin, the surfactant is sodium deoxycholate, the gel matrix is carbomer, the auxiliary material comprises humectant and preservative, the humectant and preservative account for 4% -7% and 0.1% -1% of the total mass of the hydrogel respectively, and the humectant is one or a combination of more of hyaluronic acid, glycerin and ceramide; the preservative is one or two of methylparaben and ethylparaben, the gel matrix is carbomer, the auxiliary materials are required to be added with a neutralizing agent, the neutralizing agent accounts for 0-0.3% of the total mass of the hydrogel, and the neutralizing agent is one or more of triethanolamine, diethanolamine, ethylenediamine and calcium chloride.
2. The use of an oil control polypeptide flexible liposome hydrogel according to claim 1 for preparing a skin care product having enhanced oil control efficacy of water-soluble polypeptides.
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