CN115053752A - Novel ganoderma lucidum strain cultivation method - Google Patents
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- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 25
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 25
- 238000012364 cultivation method Methods 0.000 title claims description 5
- 239000001963 growth medium Substances 0.000 claims abstract description 46
- 238000000034 method Methods 0.000 claims abstract description 22
- 230000004913 activation Effects 0.000 claims abstract description 16
- 238000012216 screening Methods 0.000 claims abstract description 16
- 241000222336 Ganoderma Species 0.000 claims abstract description 15
- 230000005855 radiation Effects 0.000 claims abstract description 14
- 239000000725 suspension Substances 0.000 claims description 26
- 230000001580 bacterial effect Effects 0.000 claims description 24
- 238000012258 culturing Methods 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 13
- 229920001817 Agar Polymers 0.000 claims description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 11
- 239000008272 agar Substances 0.000 claims description 11
- 239000008103 glucose Substances 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 239000002023 wood Substances 0.000 claims description 6
- 239000005979 Forchlorfenuron Substances 0.000 claims description 5
- GPXLRLUVLMHHIK-UHFFFAOYSA-N forchlorfenuron Chemical compound C1=NC(Cl)=CC(NC(=O)NC=2C=CC=CC=2)=C1 GPXLRLUVLMHHIK-UHFFFAOYSA-N 0.000 claims description 5
- 239000005457 ice water Substances 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 4
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- 238000002703 mutagenesis Methods 0.000 claims description 4
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- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
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- 229910052602 gypsum Inorganic materials 0.000 claims description 3
- 239000010440 gypsum Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 claims description 2
- 150000002333 glycines Chemical class 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims description 2
- 235000015099 wheat brans Nutrition 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 abstract description 8
- 229920001282 polysaccharide Polymers 0.000 abstract description 8
- 239000005017 polysaccharide Substances 0.000 abstract description 8
- 230000008901 benefit Effects 0.000 abstract description 6
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 abstract description 3
- 229910052711 selenium Inorganic materials 0.000 abstract description 3
- 239000011669 selenium Substances 0.000 abstract description 3
- 230000007547 defect Effects 0.000 abstract description 2
- 230000006698 induction Effects 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 7
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- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
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- 230000015556 catabolic process Effects 0.000 description 1
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- 238000012136 culture method Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a novel method for cultivating lucid ganoderma strains, and relates to the technical field of lucid ganoderma planting. The novel method for cultivating the ganoderma lucidum strain mainly comprises the steps of adopting a selenium-rich activation culture medium to carry out activation culture, microwave radiation induction, screening for multiple passages and the like. The method overcomes the defects of the prior art, effectively improves the polysaccharide content of ganoderma lucidum planting, improves the stability of strain passage and improves the economic benefit of planting.
Description
Technical Field
The invention relates to the technical field of ganoderma lucidum planting, in particular to a novel ganoderma lucidum strain cultivation method.
Background
Ganoderma lucidum is a precious Chinese herbal medicine which is regarded as nourishing and strengthening, and strengthening body resistance for a long time in the traditional Chinese medicine, is listed as Jiuda Mesona for 2000, and has been loaded as a legal Chinese herbal medicine in the pharmacopoeia of the people's republic of China.
The effective component in the ganoderma lucidum is ganoderma lucidum polysaccharide, the growth period of the ganoderma lucidum is long, and the economic development of artificial planting is integrally inhibited, so that the adoption of a novel planting technology is vital, as the wild ganoderma lucidum mother seeds are mostly easy to have low yield and low growth efficiency in artificial planting, and the economic benefit is poor, the wild ganoderma lucidum generally needs domestication and induction and then can be widely artificially planted, or the introduced mother seeds are directly adopted for planting, but the original seeds are transferred for many times, character degradation is easily caused in seed production, re-introduction is needed, and the polysaccharide content of different types of ganoderma lucidum is relatively different, so that how to cultivate the ganoderma lucidum strain with good stability and high medicinal value is a great important target in the ganoderma lucidum planting industry at the present stage.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a novel ganoderma lucidum strain cultivation method, which effectively improves the polysaccharide content of ganoderma lucidum planting, improves the strain passage stability and improves the planting economic benefit.
In order to achieve the above purpose, the technical scheme of the invention is realized by the following technical scheme:
a method for cultivating novel ganoderma lucidum strains comprises the following steps:
(1) preparation of an activation medium: mixing potato juice, glucose and KH 2 PO 4 、(NH 4 ) 2 SO 4 、MgSO 4 、Na 2 SeO 3 Mixing the yeast powder, agar and water to prepare an activated culture medium for later use;
(2) preparing a basic culture medium: mixing semen glycines powder, testa oryzae, testa Tritici, glucose, Gypsum Fibrosum powder, semen Maydis pulp, KH 2 PO 4 、MgSO 4 ·7H 2 Preparing a basic culture medium from O, agar, forchlorfenuron and water for later use;
(3) and (3) strain microwave mutagenesis screening: inoculating strains of the ganoderma lucidum to an activation culture medium, performing activation fermentation culture, collecting spore suspension, treating the suspension in an intermittent microwave radiation mode, and inoculating the suspension after radiation treatment to a basic culture medium for culture;
(4) subculturing: treating 5-10 batches of the bacterial colonies according to the mutagenesis mode in the step (3), screening out bacterial colonies with better morphology and growth vigor, inoculating the bacterial colonies to a new basic culture medium for continuous culture, and repeatedly carrying out passage for 4-5 times to obtain a bacterial strain with good growth as a mother strain;
(5) inoculation and cultivation: inoculating the mother seeds on wood, and culturing until hyphae grow over, thereby completing the culture.
Preferably, each substance of the culture medium is activated in the step (1)The constituents include (by weight portions) potato juice 200 parts, glucose 20 parts, KH 2 PO 4 1 part of (NH) 4 ) 2 SO 4 1 part of MgSO (MgSO) 4 1 part of Na 2 SeO 3 0.1 part, 1 part of yeast powder, 15 parts of agar and 200 parts of water.
Preferably, the basic culture medium in the step (2) comprises 40 parts by weight of soybean flour, 20 parts by weight of rice bran, 20 parts by weight of wheat bran, 15 parts by weight of glucose, 10 parts by weight of gypsum powder, 10 parts by weight of corn steep liquor and KH 2 PO 4 1 part of MgSO (MgSO) 4 ·7H 2 O1 parts, agar 6 parts, forchlorfenuron 4 parts and water 60-80 parts.
Preferably, the temperature of the activation fermentation culture in the step (3) is 30 ℃, and the culture time is 6-8 d.
Preferably, the intermittent microwave radiation in step (3) is performed by using microwave power of 750W and pulse frequency of 2400Hz, and the treatment is repeated for 3-4 times for 2-5s each time.
Preferably, the suspension is subjected to water bath heat preservation treatment by using an ice-water mixture during the microwave treatment in the step (3).
Preferably, the standards for screening colonies with good growth vigor in the step (4) are that the growth speed of mycelia is high, the color of mycelia is uniform, and the appearance is full.
Preferably, the time for the passage in the step (4) is 8-10d after culturing at 28 ℃.
Preferably, the mother seeds in step (4) take growth rate as a screening criterion.
The invention provides a method for cultivating novel ganoderma lucidum strains, which has the advantages that:
(1) in the invention, Na 2 SeO 3 The strain with strong selenium resistance and selenium enrichment capacity can be screened in the subsequent culture by adding the strain into an activation culture medium, and meanwhile, the strain is screened after being induced by microwave radiation, so that the obtained strain has higher polysaccharide yield and the medicinal effect is effectively improved;
(2) the invention obtains the strain with good passage capacity by subculturing the strain after microwave radiation, effectively prevents the strain from degeneration in the actual multi-generation planting process, improves the planting stability of the strain and ensures the economic benefit of planting.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are described below clearly and completely in conjunction with the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
preparation of an activation medium: collecting potato juice 200g, glucose 20g, and 1gKH 2 PO 4 、0.1gNa 2 SeO 3 1g of yeast powder, 1g of (NH) 4 ) 2 SO 4 、1gMgSO 4 Mixing 15g of agar and 150ml of water to prepare an activation culture medium;
general medium: collecting potato juice 200g, glucose 20g, and 1gKH 2 PO 4 1g of yeast powder, 1g of (NH) 4 ) 2 SO 4 、1gMgSO 4 Mixing 15g of agar and 150ml of water to prepare a common culture medium;
preparing a basic culture medium: collecting 40g bean flour, 20g rice bran, 20g bran, 15g glucose, 10g gypsum powder, 10g corn steep liquor, 1gKH 2 PO 4 、1gMgSO 4 ·7H 2 O, 6g of agar, 4g of forchlorfenuron and 70ml of water are mixed to prepare a basic culture medium.
Example 2:
culturing lucid ganoderma strains:
(1) inoculating the south Korean ganoderma lucidum strain to the activation culture medium in the embodiment 1, culturing at 30 ℃ for 7 days until hyphae grow on the surface, taking sterile normal saline to the surface of the culture medium, lightly brushing spores on the surface by using a sterile brush, collecting spore suspension, adding the sterile normal saline for dilution, and uniformly shaking to obtain spore suspension for later use;
(2) taking 10 groups of spore suspensions, placing 10ml of each group into a test tube, placing the test tube into a beaker filled with ice-water mixture for water bath, adjusting the microwave power to 700W, carrying out microwave radiation treatment after the pulse frequency is 2400Hz, repeatedly treating for 4 times, treating 8 batches of spore suspensions according to the step, respectively taking 100 mu L of the suspension subjected to radiation treatment, inoculating the suspension into the basic culture medium in the embodiment 1, culturing for 10d at the temperature of 28 ℃, screening out bacterial colonies with better morphology and growth vigor, and inoculating the bacterial colonies onto a new basic culture medium for continuous culture;
(3) selecting several groups of bacterial colonies with the highest growth rate from the continuously cultured culture medium, distributing the bacterial colonies to a new basic culture medium for passage, repeating the passage for 4 times, and screening out bacterial strains with good growth as mother strains;
(4) inoculating the mother seeds on wood, and culturing until hyphae grow over, thereby completing the culture.
Comparative example 1:
culturing lucid ganoderma strains:
(1) inoculating the south Korean ganoderma lucidum strain to the activation culture medium in the embodiment 1, culturing at 30 ℃ for 7 days until hyphae grow on the surface, taking sterile normal saline to the surface of the culture medium, lightly brushing spores on the surface by using a sterile brush, collecting spore suspension, adding the sterile normal saline for dilution, and uniformly shaking to obtain spore suspension for later use;
(2) inoculating 100 μ L of the spore suspension into the basic culture medium of example 1, culturing at 28 deg.C for 10 days, and inoculating the selected colony with good morphology and growth to new basic culture medium for further culture;
(3) selecting several groups of bacterial colonies with the highest growth rate from the continuously cultured culture medium, distributing the bacterial colonies to a new basic culture medium for passage, repeating the passage for 4 times, and screening out bacterial strains with good growth as mother strains;
(4) inoculating the mother seeds on wood, and culturing until hyphae grow over, thereby completing the culture.
Comparative example 2
Culturing lucid ganoderma strains:
(1) inoculating south Korean Ganoderma strain to the common culture medium in example 1, culturing at 30 deg.C for 7d until mycelia grow on the surface, applying sterile physiological saline to the surface of the culture medium, brushing spores on the surface with sterile brush, collecting spore suspension, diluting with sterile physiological saline, shaking, and mixing to obtain spore suspension;
(2) taking 10 groups of spore suspensions, placing 10ml of each group into a test tube, placing the test tube into a beaker filled with ice-water mixture for water bath, adjusting the microwave power to 700W, carrying out microwave radiation treatment after the pulse frequency is 2400Hz, repeatedly treating for 4 times, treating 8 batches of spore suspensions according to the step, respectively taking 100 mu L of the suspension subjected to radiation treatment, inoculating the suspension into the basic culture medium in the embodiment 1, culturing for 10d at the temperature of 28 ℃, screening out bacterial colonies with better morphology and growth vigor, and inoculating the bacterial colonies onto a new basic culture medium for continuous culture;
(3) selecting several groups of bacterial colonies with the highest growth rate from the continuously cultured culture medium, distributing the bacterial colonies to a new basic culture medium for passage, repeating the passage for 4 times, and screening out bacterial strains with good growth as mother strains;
(4) inoculating the mother seeds on wood, and culturing until hyphae grow over, thereby completing the culture.
Comparative example 3
Culturing lucid ganoderma strains:
(1) inoculating the south Korean ganoderma lucidum strain to the common culture medium in the embodiment 1, culturing at 30 ℃ for 7 days until hyphae grow on the surface, taking sterile normal saline to the surface of the culture medium, lightly brushing spores on the surface by using a sterile brush, collecting spore suspension, adding the sterile normal saline for dilution, and uniformly shaking to obtain the spore suspension for later use;
(2) inoculating 100 μ L of the spore suspension into the basic culture medium of example 1, culturing at 28 deg.C for 10 days, selecting colony with good morphology and growth, inoculating to new basic culture medium, and culturing;
(3) selecting several groups of bacterial colonies with the highest growth rate from the continuously cultured culture medium, distributing the bacterial colonies to a new basic culture medium for passage, repeating the passage for 4 times, and screening out bacterial strains with good growth as mother strains;
(4) inoculating the mother seeds on wood, and culturing until hyphae grow over, thereby completing the culture.
And (3) detection:
1. the mother strains of the above example 2 and comparative examples 1-3 were subjected to shake flask fermentation tests to determine the dry weight of the mycelia and the exopolysaccharide content, and the results are shown in the following table 1:
TABLE 1
| Group of | Mycelium dry weight (g/L) | Extracellular polysaccharide content (g/L) |
| Example 2 | 13.6 | 3.8 |
| Comparative example 1 | 13.5 | 3.1 |
| Comparative example 2 | 13.4 | 3.0 |
| Comparative example 3 | 12.9 | 2.8 |
As can be seen from the above table, Na is used 2 SeO 3 The thalli obtained by combined treatment of screening and microwave radiation has higher extracellular polysaccharide content.
Shaking flask fermentation test was performed on each generation of the strain passaged in step (3) of the above example 2, and the dry weight of mycelia and the content of exopolysaccharides of the strain were measured for 1-4 generations, and the results are shown in the following table 2:
TABLE 2
| Group of | Mycelium dry weight (g/L) | Extracellular polysaccharide content (g/L) |
| First generation | 13.6 | 3.7 |
| Second generation | 13.5 | 3.8 |
| Third generation | 13.7 | 3.9 |
| Fourth generation | 13.6 | 3.8 |
As can be seen from the above table, the culture method of example 2 is adopted, and the strain performance of the passage has good stability.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (9)
1. A cultivation method of a novel ganoderma lucidum strain is characterized by comprising the following steps:
(1) preparation of an activation medium: mixing potato juice, glucose and KH 2 PO 4 、(NH 4 ) 2 SO 4 、MgSO 4 、Na 2 SeO 3 Mixing the yeast powder, agar and water to prepare an activated culture medium for later use;
(2) preparing a basic culture medium: mixing semen glycines powder, testa oryzae, testa Tritici, glucose, Gypsum Fibrosum powder, semen Maydis pulp, KH 2 PO 4 、MgSO 4 ·7H 2 Preparing a basic culture medium from O, agar, forchlorfenuron and water for later use;
(3) and (3) strain microwave mutagenesis screening: inoculating strains of the ganoderma lucidum to an activation culture medium, performing activation fermentation culture, collecting spore suspension, treating the suspension by adopting an intermittent microwave radiation mode, and inoculating the suspension after radiation treatment to a basic culture medium for culture;
(4) subculturing: treating 5-10 batches of the bacterial colonies according to the mutagenesis mode in the step (3), screening out bacterial colonies with better morphology and growth vigor, inoculating the bacterial colonies to a new basic culture medium for continuous culture, and repeatedly carrying out passage for 4-5 times to obtain a bacterial strain with good growth as a mother strain;
(5) inoculating and culturing: inoculating the mother seeds on wood, and culturing until hyphae grow over, thereby completing the culture.
2. The method for cultivating a novel ganoderma species according to claim 1, wherein: the components of the activation culture medium in the step (1) comprise, by weight, 200 parts of potato juice, 20 parts of glucose and KH 2 PO 4 1 part of (NH) 4 ) 2 SO 4 1 part of MgSO (MgSO) 4 1 part of Na 2 SeO 3 0.1 part, 1 part of yeast powder, 15 parts of agar and 200 parts of water.
3. The method for cultivating a novel ganoderma species according to claim 1, wherein: the basic culture medium in the step (2) comprises 40 parts of soybean flour, 20 parts of rice bran, 20 parts of wheat bran, 15 parts of glucose, 10 parts of gypsum powder, 10 parts of corn steep liquor and KH 2 PO 4 1 part of MgSO (MgSO) 4 ·7H 2 O1 parts, agar 6 parts, forchlorfenuron 4 parts and water 60-80 parts.
4. The method for cultivating a novel ganoderma species according to claim 1, wherein: the temperature of the activation fermentation culture in the step (3) is 30 ℃, and the culture time is 6-8 d.
5. The method for cultivating a novel ganoderma species according to claim 1, wherein: the intermittent microwave radiation process in the step (3) adopts 750W microwave power and 2400Hz pulse frequency, and the treatment is repeated for 3-4 times for 2-5s each time.
6. The method for cultivating a novel ganoderma species according to claim 1, wherein: and (4) performing water bath heat preservation treatment on the suspension by using an ice-water mixture in the microwave treatment process in the step (3).
7. The method for cultivating a novel ganoderma species according to claim 1, wherein: the standards for screening colonies with good growth vigor in the step (4) are that the growth speed of mycelia is high, the color of the mycelia is uniform, and the appearance is full.
8. The method for cultivating a novel ganoderma species according to claim 1, wherein: and (4) carrying out passage after culturing at the temperature of 28 ℃ for 8-10 d.
9. The method for cultivating a novel ganoderma species according to claim 1, wherein: and (4) selecting the mother seeds in the step (4) by taking the growth rate as a screening standard.
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| CN103477994A (en) * | 2013-06-28 | 2014-01-01 | 江苏大学 | Bacterial strain used for producing ganoderma lucidum polysaccharides by complete feed liquid fermentation of rice bran and wheat bran |
| CN114214208A (en) * | 2021-12-14 | 2022-03-22 | 江南大学 | High-selenium-resistance ganoderma lucidum mutant strain and application thereof |
| CN114250153A (en) * | 2021-12-15 | 2022-03-29 | 江南大学 | High-selenium-resistance Ganoderma lucidum JNUSE-200 and selenium-rich fermentation strategy thereof |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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