CN115029238A - Experimental device and method for researching biological effect mechanism of pulsed magnetic field - Google Patents
Experimental device and method for researching biological effect mechanism of pulsed magnetic field Download PDFInfo
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Abstract
The invention discloses an experimental device and method for researching a pulse magnetic field biological effect mechanism, relates to an experimental device and method for stimulating a pulse magnetic field of an experimental animal or a cell, and aims to solve the problem that the conventional myocardial infarction research is not based on a magnetic field pulse stimulation device; magnetic field generating means for generating a magnetic field; the direct current power supply is connected with the magnetic field generating device and used for providing pulse current for the magnetic field generating device so that the magnetic field generated by the magnetic field generating device is a pulse magnetic field; the experimental platform is positioned in the range of the pulse magnetic field and used for placing experimental materials; and the monitoring system is used for acquiring the magnetic field intensity of the pulse magnetic field and the temperature in the pulse magnetic field.
Description
Technical Field
The invention relates to an experimental device and method for stimulating experimental animals or cells by a pulsed magnetic field.
Background
Although the revascularization operation can restore the blood flow of myocardial infarction, irreversible damage to myocardial cells and local inflammatory reaction are still difficult to avoid and become main factors of poor prognosis of myocardial infarction. The pulsed electromagnetic field biological treatment relates to the crossing fields of physics, genetic engineering, material science and medicine, and has wide application prospect in regulating and controlling cell biological functions. The scholars at home and abroad carry out research around the problem and find that the pulse electromagnetic field inhibits the generation of active oxygen and has the anti-inflammatory and antioxidant effects. The active oxygen generated during myocardial infarction promotes the further expansion of the myocardial damage range, and the inhibition effect of the pulse electromagnetic field on the active oxygen suggests the potential value of the pulse electromagnetic field in the myocardial infarction treatment. But no device based on magnetic field pulse stimulation is researched on myocardial infarction at present.
Disclosure of Invention
The invention aims to solve the problem that the conventional myocardial infarction research is not provided with a device based on magnetic field pulse stimulation, and provides a pulsed magnetic field biological effect mechanism research experimental device.
The invention provides a pulsed magnetic field biological effect mechanism research experimental device, which comprises a magnetic field generating device, a direct-current power supply, an experimental platform and a monitoring system, wherein the magnetic field generating device is connected with the direct-current power supply;
magnetic field generating means for generating a magnetic field;
the direct current power supply is connected with the magnetic field generating device and used for providing pulse current for the magnetic field generating device so that the magnetic field generated by the magnetic field generating device is a pulse magnetic field;
the experimental platform is positioned in the range of the pulse magnetic field and used for placing experimental materials;
and the monitoring system is used for acquiring the magnetic field intensity of the pulse magnetic field and the temperature in the pulse magnetic field.
Wherein, also include the shielding system of the magnetic field;
and the magnetic field shielding system is enclosed outside the magnetic field generating device and is used for shielding the magnetic field of the external environment.
The magnetic field generating device comprises a pair of X axial magnetic field coils, a pair of Y axial magnetic field coils and a pair of Z axial magnetic field coils; the X axial magnetic field coil and the Y axial magnetic field coil are annular frustum-shaped coils with the same specification, and the Z axial magnetic field coil is an annular coil;
the pair of X axial magnetic field coils are symmetrically arranged, and the central axes of the pair of X axial magnetic field coils are positioned on a straight line parallel to the X axis;
the pair of Y-axis magnetic field coils are symmetrically arranged, and the central axes of the pair of Y-axis magnetic field coils are positioned on a straight line parallel to the Y axis;
the central axis of the X axial magnetic field coil and the central axis of the Y axial magnetic field coil are both positioned on an XOY plane;
a pair of Z axial magnetic field coils are symmetrically arranged above and below the plane, and the central axes of the pair of Z axial magnetic field coils are all positioned on a straight line parallel to the Z axis.
Wherein the minimum regulation resolution of the pulse current is less than or equal to 10nA, and the stability is less than or equal to 1%/h;
the pulse magnetic field intensity is 1 uT-100 mT, the minimum adjustment resolution of the pulse magnetic field is 10nT, the frequency is 0-300 Hz, the minimum adjustable resolution of the frequency is 0.1Hz, and the minimum adjustable duty ratio of the pulse magnetic field under 300Hz is 1%.
Wherein the magnetic field fluctuation in the pulsed magnetic field is less than or equal to 10 nT.
The width of the experimental platform is matched with the inner diameter of the Z-axis magnetic field coil, and the experimental platform is positioned in the center of the magnetic field generating device.
Wherein, the experiment platform is a PVC plate.
The invention also provides a pulsed magnetic field biological effect mechanism research experimental method, which is based on the pulsed magnetic field biological effect mechanism research experimental device and comprises the following specific steps:
placing an experimental animal or an experimental cell on an experimental platform, outputting pulse current through a direct current power supply, controlling a magnetic field generating device to generate a pulse magnetic field, and performing pulse magnetic field intervention on the experimental animal or the experimental cell under the pulse magnetic field with set strength and frequency;
and step two, carrying out subsequent detection of experimental animal or experimental cell research.
Wherein, still include:
recording magnetic field stimulation values through a monitoring system when the pulsed magnetic field intervention is carried out on the experimental animal or the experimental cell under the pulsed magnetic field with set intensity and frequency, wherein the magnetic field stimulation values comprise the intensity and the frequency of the pulsed magnetic field and the temperature in the pulsed magnetic field; and repeatedly executing the steps I to II for a set number of times through the magnetic field stimulation value.
The invention has the beneficial effects that:
a device and a method for researching and testing the biological effect mechanism of a pulse magnetic field realize three-in-one of magnetic field generation, monitoring and environmental magnetic field shielding, can provide a stable and uniform magnetic field, simultaneously display the internal strength and temperature of the magnetic field, and shield the interference of the surrounding environmental magnetic field. The pulsed electromagnetic stimulation can be applied to animal models and in vitro cultured cells through experimental observation. Provides a platform for the research of the biological effect mechanism of the pulsed magnetic field.
Drawings
FIG. 1 is a schematic structural diagram of an experimental apparatus for studying the biological effect mechanism of a pulsed magnetic field according to the present invention;
FIG. 2 is a schematic structural diagram of a magnetic field generator in an experimental apparatus for studying the biological effect mechanism of a pulsed magnetic field according to the present invention;
FIG. 3 is a schematic diagram of an animal experiment and an in vitro cell experiment performed by using the experimental device for researching the biological effect mechanism of the pulsed magnetic field according to the present invention;
FIG. 4 is a schematic diagram of the effect of reducing the myocardial infarction area by pulse stimulation of the experimental device for studying the biological effect mechanism of the pulsed magnetic field of the invention; the figure shows TTC staining results of myocardial tissues of various groups of mice, wherein a pale area is an infarct area, and a statistical histogram of myocardial infarction areas of the mice shows that the infarct areas are reduced by the stimulation of the PEMF pulse electromagnetic field; n is 6, # P <0.05vs. sham, and # P <0.05vs. ami; note: sham is a control group, AMI is a myocardial infarction group, and PEMF is a pulse electromagnetic field;
fig. 5 is a schematic diagram of the effect of using the experimental device for researching the biological effect mechanism of the pulsed magnetic field to perform pulse stimulation on myocardial infarction to improve the left ventricular contraction function; the figure is an echocardiogram, the PEMF pulse electromagnetic field stimulation can improve left ventricular Ejection Fraction (EF) and short axis shrinkage (FS) of an AMI mouse, n is 6, P is less than 0.05vs. sham, and P is less than 0.05vs. AMI; sham is the control group, AMI is the myocardial infarction group, and PEMF is the pulsed electromagnetic field.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The invention is further described with reference to the following drawings and specific examples, which are not intended to be limiting.
In a first specific embodiment, the experimental apparatus for researching a biological effect mechanism of a pulsed magnetic field in this embodiment includes a magnetic field generating device 1, a dc power supply 2, an experimental platform 3 and a monitoring system 4;
a magnetic field generating device 1 for generating a magnetic field;
the direct current power supply 2 is connected with the magnetic field generating device 1 and is used for providing pulse current for the magnetic field generating device 1 so that the magnetic field generated by the magnetic field generating device 1 is a pulse magnetic field;
the experiment platform 3 is positioned in the range of the pulse magnetic field and used for placing experiment materials;
and the monitoring system 4 is used for acquiring the magnetic field intensity of the pulse magnetic field and the temperature in the pulse magnetic field.
Specifically, aiming at the phenomenon of myocardial infarction cell damage, a pulsed magnetic field stimulation system is provided for researching the myocardial infarction damage mechanism.
As shown in fig. 1, the device includes a magnetic field generating device 1, a programmable dc power supply 2, a monitoring system 4, and an experimental platform 3 for placing experimental animals.
The programmable direct current power supply 2 has a remote control function and a pulse function, and can supply pulse current to the magnetic field generating device 1 to generate a pulse magnetic field.
The monitoring system 4 can monitor the magnetic field intensity and the temperature in the field in real time and transmit monitoring data to an external display device.
Best embodiment, this embodiment is a further description of the first embodiment, and in this embodiment, the magnetic shielding system 5 is further included;
and the magnetic field shielding system 5 is enclosed outside the magnetic field generating device 1 and is used for shielding the external environment magnetic field.
Further, the magnetic field shielding system 5 can shield the interfering magnetic field outside the room (outside the magnetic field generating device 1) and ensure that the pulsed magnetic field inside the shielded room (inside the magnetic field generating device 1) is not affected by the external magnetic field.
In the present embodiment, the magnetic field generating device 1 includes a pair of X axial magnetic field coils 1-1, a pair of Y axial magnetic field coils 1-2, and a pair of Z axial magnetic field coils 1-3; the X axial magnetic field coil 1-1 and the Y axial magnetic field coil 1-2 are annular frustum-shaped coils with the same specification, and the Z axial magnetic field coil 1-3 is an annular coil;
a pair of X axial magnetic field coils 1-1 are symmetrically arranged, and the central axes of the pair of X axial magnetic field coils 1-1 are all positioned on a straight line parallel to the X axis;
the pair of Y-axis magnetic field coils 1-2 are symmetrically arranged, and the central axes of the pair of Y-axis magnetic field coils 1-2 are all positioned on a straight line parallel to the Y axis;
the central axis of the X axial magnetic field coil 1-1 and the central axis of the Y axial magnetic field coil 1-2 are both positioned on an XOY plane;
the pair of Z-axis magnetic field coils 1-3 are symmetrically arranged above and below the plane, and the central axes of the pair of Z-axis magnetic field coils 1-3 are all positioned on a straight line parallel to the Z axis.
Specifically, the magnetic field generating device 1 is a three-axis Helmholtz coil made of copper wire, and the compact three-axis uniform field coil is designed as shown in FIGS. 2 to 3, and is magnetically excited in the X axis directionThe field coil 1-1, the Y axial magnetic field coil 1-2, the Z axial magnetic field coil 1-3 triaxial coil is constituteed, the Z axial magnetic field coil 1-3 comprises two cake shape coils, X axial magnetic field coil 1-1, Y axial magnetic field coil 1-2 are respectively by the coil of two annular frustum form coils and constitute, X axial magnetic field coil 1-1, Y axial magnetic field coil 1-2, Z axial magnetic field coil 1-3 triaxial coil can realize unipolar control, can realize arbitrary direction magnetic field control. For example, under the conditions that the outer diameter of the Z-axis magnetic field coil 1-3 is 10cm and the inner diameter is 4cm, the magnetic field obtained by superimposing the magnetic field vectors generated by the three-axis coils has a magnetic field strength difference of the pT level at the position where the magnetic induction deviation from the center point is the largest in the region of a 5mm × 5mm × 5mm cube at the center, and the earth magnetic field strength is 50 to 60 μ T, that is, the magnetic field generating apparatus 1 is 5 × 5 × 5mm 3 The difference in magnetic field generated in the region is of the order of 10-6 of the natural magnetic field and can be considered as a nearly completely uniform magnetic field distribution.
In the present embodiment, the pulse current is 90uA to 10mA, the ripple is less than or equal to 0.9uA, the minimum adjustment resolution is less than or equal to 10nA, and the stability is less than or equal to 1%/h;
the pulse magnetic field intensity is 1 uT-100 mT, the minimum adjustment resolution of the pulse magnetic field is 10nT, the frequency is 0-300 Hz, the minimum adjustable resolution of the frequency is 0.1Hz, and the minimum adjustable duty ratio of the pulse magnetic field under 300Hz is 1%.
Best mode for carrying out the invention this example is a further description of the first embodiment, and in this example, the magnetic field fluctuation in the pulsed magnetic field is 10nT or less.
In this embodiment, the width of the experimental platform 3 is matched with the inner diameter of the Z-axis magnetic field coils 1-3, and the experimental platform 3 is located at the center of the magnetic field generating device 1.
Best mode for carrying out the invention this example is a further description of the first embodiment, in this example, the experiment platform 3 is a PVC plate.
Specifically, as shown in fig. 3, the width of the PVC plate is smaller than the inner diameter of the Z-axis magnetic field coil 1-3, the PVC plate is located at a position perpendicular to the end faces of the X-axis magnetic field coil 1-1 and the Y-axis magnetic field coil 1-2, and at the center of the Z-axis magnetic field coil 1-3. And the magnetic field of the PVC plate (including experimental animals or experimental cells) is required to be characterized by good internal field intensity uniformity and magnetic field fluctuation less than 10 nT.
In a second specific embodiment, the pulsed magnetic field biological effect mechanism research experimental method in this embodiment is based on the pulsed magnetic field biological effect mechanism research experimental apparatus, and includes the following specific steps:
firstly, placing an experimental animal or an experimental cell on an experimental platform 3, outputting pulse current through a direct current power supply 2, controlling a magnetic field generating device 1 to generate a pulse magnetic field, and performing pulse magnetic field intervention on the experimental animal or the experimental cell under the pulse magnetic field with set strength and frequency;
and step two, carrying out subsequent detection of experimental animal or experimental cell research.
Step three, when the pulsed magnetic field intervention is carried out on the experimental animal or the experimental cell under the pulsed magnetic field with set intensity and frequency, the magnetic field stimulation value is recorded through the monitoring system 4, and the magnetic field stimulation value comprises the intensity and the frequency of the pulsed magnetic field and the temperature in the pulsed magnetic field; and the steps one to two of the set times are repeatedly executed through the magnetic field stimulation value.
Specifically, the experimental device for researching the biological effect mechanism of the pulsed magnetic field can be used as various animal models and cell models, and the influence of the pulsed geomagnetism with different frequencies and field strengths on various systems of organisms is researched by changing the field strength and the frequency. For example, the experimental study method is as follows.
(1) The anesthetized mouse (experimental animal) or in-vitro cultured cells (experimental cells) are placed on an experimental platform 3 in a magnetic field generating device 1, and an upper computer remotely controls a direct current power supply 2 to enable the frequency of a pulse magnetic field generated by the direct current power supply to be 15Hz and the magnetic field intensity to be 1 mT;
the time and parameters may also be adjusted according to experimental design.
(2) Real magnetic field stimulation values in the research are recorded through real-time magnetic field intensity and temperature monitoring values transmitted to the display by the monitoring system 4, and repeatability of experimental research is guaranteed.
(3) The whole experimental process is completed in the magnetic shielding system 5. The interference of an environmental magnetic field is avoided, and a high-purity magnetic field biological effect research environment is provided.
(4) The myocardial infarction animal model for experimental study is subjected to pulsed magnetic field intervention for a certain period before or after modeling, then the myocardial infarction area is judged by a TTC staining method (as shown in figure 4), and the left ventricular function of the animal is tested by ultrasound (as shown in figures 5A, 5B and 5C).
Although the invention herein has been described with reference to particular embodiments, it is to be understood that these embodiments are merely illustrative of the principles and applications of the present invention. It is therefore to be understood that numerous modifications may be made to the illustrative embodiments and that other arrangements may be devised without departing from the spirit and scope of the present invention as defined by the appended claims. It should be understood that features described in different dependent claims and herein may be combined in ways different from those described in the original claims. It is also to be understood that features described in connection with individual embodiments may be used in other described embodiments.
Claims (9)
1. A pulsed magnetic field biological effect mechanism research experimental device is characterized by comprising a magnetic field generating device (1), a direct current power supply (2), an experimental platform (3) and a monitoring system (4);
the magnetic field generating device (1) is used for generating a magnetic field;
the direct current power supply (2) is connected with the magnetic field generating device (1) and is used for providing pulse current to the magnetic field generating device (1) so that the magnetic field generated by the magnetic field generating device (1) is a pulse magnetic field;
the experiment platform (3) is positioned in the range of the pulse magnetic field and is used for placing experiment materials;
and the monitoring system (4) is used for acquiring the magnetic field intensity of the pulse magnetic field and the temperature in the pulse magnetic field.
2. The experimental device for researching biological effect mechanism of the pulsed magnetic field according to claim 1, characterized by further comprising a magnetic field shielding system (5);
the magnetic field shielding system (5) is enclosed outside the magnetic field generating device (1) and is used for shielding the magnetic field of the external environment.
3. The experimental device for researching biological effect mechanism of the pulsed magnetic field according to claim 1, wherein the magnetic field generating device (1) comprises a pair of X axial magnetic field coils (1-1), a pair of Y axial magnetic field coils (1-2) and a pair of Z axial magnetic field coils (1-3); the X axial magnetic field coil (1-1) and the Y axial magnetic field coil (1-2) are annular frustum-shaped coils with the same specification, and the Z axial magnetic field coil (1-3) is an annular coil;
the pair of X axial magnetic field coils (1-1) are symmetrically arranged, and the central axes of the pair of X axial magnetic field coils (1-1) are all positioned on a straight line parallel to the X axis;
the pair of Y-axis magnetic field coils (1-2) are symmetrically arranged, and the central axes of the pair of Y-axis magnetic field coils (1-2) are all positioned on a straight line parallel to the Y axis;
the central axis of the X axial magnetic field coil (1-1) and the central axis of the Y axial magnetic field coil (1-2) are both positioned on an XOY plane;
the pair of Z-axis magnetic field coils (1-3) are symmetrically arranged above and below the plane, and the central axes of the pair of Z-axis magnetic field coils (1-3) are all located on a straight line parallel to the Z axis.
4. The experimental device for researching biological effect mechanism of the pulsed magnetic field according to claim 1, 2 or 3, characterized in that the minimum regulating resolution of the pulse current is less than or equal to 10nA, and the stability is less than or equal to 1%/h;
the pulse magnetic field intensity is 1 uT-100 mT, the minimum adjustment resolution of the pulse magnetic field is 10nT, the frequency is 0-300 Hz, the minimum resolution of the adjustable frequency is 0.1Hz, and the minimum adjustable duty ratio of the pulse magnetic field under 300Hz is 1%.
5. The experimental device for researching biological effect mechanism of pulsed magnetic field according to claim 4, wherein the magnetic field fluctuation inside the pulsed magnetic field is less than or equal to 10 nT.
6. The experimental device for researching biological effect mechanism of the pulsed magnetic field according to claim 3, wherein the width of the experimental platform (3) is matched with the inner diameter of the Z-axis magnetic field coil (1-3), and the experimental platform (3) is located at the central position of the magnetic field generating device (1).
7. The experimental device for researching biological effect mechanism of pulsed magnetic field according to claim 6, characterized in that the experimental platform (3) is PVC plate.
8. A pulsed magnetic field biological effect mechanism research experimental method is characterized in that based on the pulsed magnetic field biological effect mechanism research experimental device of claim 5, the specific steps are as follows:
placing an experimental animal or an experimental cell on an experimental platform (3), outputting pulse current through a direct current power supply (2), controlling a magnetic field generating device (1) to generate a pulse magnetic field, and performing pulse magnetic field intervention on the experimental animal or the experimental cell under the pulse magnetic field with set strength and frequency;
and step two, carrying out subsequent detection of experimental animal or experimental cell research.
9. The experimental method for researching the biological effect mechanism of the pulsed magnetic field according to claim 8, further comprising:
recording magnetic field stimulation values through a monitoring system (4) when the pulsed magnetic field intervention is carried out on the experimental animal or the experimental cell under the pulsed magnetic field with set intensity and frequency, wherein the magnetic field stimulation values comprise the intensity and the frequency of the pulsed magnetic field and the temperature in the pulsed magnetic field; and repeatedly executing the steps I to II for a set number of times through the magnetic field stimulation value.
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Citations (5)
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| US6443883B1 (en) * | 1999-06-08 | 2002-09-03 | Medical Bracing Systems, Ltd. | PEMF biophysical stimulation field generator device and method |
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2022
- 2022-06-08 CN CN202210645613.5A patent/CN115029238A/en active Pending
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| US6443883B1 (en) * | 1999-06-08 | 2002-09-03 | Medical Bracing Systems, Ltd. | PEMF biophysical stimulation field generator device and method |
| JP2004290180A (en) * | 2003-03-10 | 2004-10-21 | Japan Science & Technology Agency | Magnetic field stimulation device, and method for promoting or inhibiting growth of living body cell or tissue using the magnetic field stimulating device |
| WO2005071057A1 (en) * | 2004-01-22 | 2005-08-04 | Japan Science And Technology Agency | Magnetic or electric field stimulating device and method for promoting, restraining, or obstructing growth and function of living cell or living tissue using the magnetic or electric field stimulating device |
| CN101324552A (en) * | 2008-04-15 | 2008-12-17 | 中国人民解放军第四军医大学 | Design method of magnetic field biology effect experiment instrument |
| CN215250921U (en) * | 2021-05-08 | 2021-12-21 | 四川大学 | Device for magnetic field stimulation and electric field stimulation of cells |
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| 李松山: "《医学物理学》", 31 March 2009, 冶金工业出版社 * |
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