CN215250921U - Device for magnetic field stimulation and electric field stimulation of cells - Google Patents
Device for magnetic field stimulation and electric field stimulation of cells Download PDFInfo
- Publication number
- CN215250921U CN215250921U CN202120973082.3U CN202120973082U CN215250921U CN 215250921 U CN215250921 U CN 215250921U CN 202120973082 U CN202120973082 U CN 202120973082U CN 215250921 U CN215250921 U CN 215250921U
- Authority
- CN
- China
- Prior art keywords
- field stimulation
- sample
- slide
- magnet
- electric field
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a device for being directed at cell carries out magnetic field stimulation and electric field stimulation relates to cell experiment technical field, and mainly used solves the problem that the cell that lacks suitable device at present and cultivate on biomaterial or animal tissue carries out magnetic field stimulation and electric field stimulation experiment. The device main structure does: including ability axial pivoted U type electro-magnet, still be equipped with slide fixed platform between two magnetic poles of U type electro-magnet, the last removable sample slide of installing of slide fixed platform, sample slide upper surface is equipped with two conductive pin, still is equipped with many sample groove between two conductive pin, and sample groove bottom is equipped with the conducting bar, and the conducting bar both ends are connected with two conductive pin respectively. The utility model provides a pair of a device for being directed at cell carries out magnetic field stimulation and electric field stimulation, through the device, the cell that the staff can be very convenient cultivates on biomaterial or animal tissue carries out magnetic field stimulation and electric field stimulation experiment.
Description
Technical Field
The utility model relates to a cell experiment technical field especially relates to a device for being used for carrying out magnetic field stimulation and electric field stimulation to the cell.
Background
The effect of magnetic stimulation on organisms is complex, which needs to comprehensively know the action mechanism of magnetic stimulation on organisms, explore the action rule of magnetic field stimulation on partial organs and cells serving as biological units, select proper magnetic field parameters, scientifically utilize magnetic fields and better treat human diseases.
Bioelectricity is the basic attribute of life activity, and moderate electric field stimulation can promote the activity, proliferation and directional movement of cells, thereby accelerating wound repair. The electric field stimulation can promote the skin fibroblast proliferation, the expression increase of transforming growth factor beta 1 protein, the deposition and the remodeling of alpha smooth muscle actin and type I collagen, thereby accelerating the healing of skin wounds. Electric field stimulation not only affects the metabolism and remodeling of the extracellular matrix, but also regulates a range of factors associated with tissue remodeling, such as up-regulation of cellular activity, migration, and protein synthesis rate.
More and more research is now being conducted from the point of studying cells, horizontal culture, to the behavior of biological materials or tissues. The diversity and complexity of biomaterials has made it difficult for current devices to meet the requirements of magnetic and electric field stimulation experiments on cells cultured on biomaterials or animal tissues.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to provide a device for carrying out magnetic field stimulation and electric field stimulation to the cell, through the device, the cell that the staff can be very convenient cultivates on biomaterial or animal tissue carries out magnetic field stimulation and electric field stimulation experiment.
The utility model provides a technical scheme of above-mentioned technical problem is: the utility model provides a device for being directed at cell carries out magnetic field stimulation and electric field stimulation, includes that the level sets up and axial pivoted U type electro-magnet, it is equipped with a slide fixed platform to go back the level between two magnetic poles of U type electro-magnet, removable horizontal installation has sample slide on slide fixed platform's the mesa, sample slide upper surface level is equipped with two and the conductive pin of magnetic pole syntropy extension and one end and power connection of U type electro-magnet, two sample slide upper surface level between the conductive pin is equipped with many and the magnetic pole extending direction vertically sample groove of U type electro-magnet, sample tank bottom is equipped with the busbar, the busbar both ends extend to the sample groove outside and be connected with two conductive pins respectively.
As a further improvement of the utility model, one side of the slide fixing platform, which is far away from the U-shaped electromagnet, is connected with one end of a platform rotating shaft which is horizontally arranged and the extending direction of which is vertical to the extending direction of the sample groove, and the other end of the platform rotating shaft is in transmission connection with the power output end of a platform rotating motor; the lower part of the platform rotating motor is fixedly arranged on the workbench, and the workbench is provided with a motor controller connected with the platform rotating motor through a lead.
As a further improvement of the utility model, one side of the U-shaped electromagnet far away from the slide fixing platform is connected with one end of a magnet rotating shaft which is horizontally arranged and the extending direction of which is vertical to the extending direction of the sample groove, and the other end of the magnet rotating shaft is in transmission connection with the power output end of a magnet rotating motor; the lower part of the magnet rotating motor is fixedly arranged on the workbench, and the workbench is provided with a motor controller connected with a magnet rotating motor through a lead.
As the utility model discloses a further improvement, the junction of magnet pivot and U type electro-magnet is equipped with a vertical magnet fixed block of arranging that is used for connecting both, the magnet fixed block is close to and is equipped with the magnet fixed block on the face of U type electro-magnet one side, magnet fixed block middle part is equipped with the magnet draw-in groove, magnet fixed block upper portion still is equipped with perpendicular setting and the lower extreme extends to the inboard locking bolt of magnet draw-in groove, locking bolt is equipped with the stay on the terminal surface with U type electro-magnet contact.
As a further improvement of the utility model, the mesa upper level of slide fixed platform of sample slide below is equipped with the mounting groove that passes whole mesa, the extending direction of mounting groove is perpendicular with the extending direction of electrically conductive pin.
As a further improvement of the utility model, the upper parts of the side walls at the two sides of the mounting groove are provided with slide fixing steps matched with the edges at the two sides of the sample slide.
As a further improvement of the utility model, two the one end that electrically conductive pin and power are connected extends to sample slide edge outside, be equipped with on the inside wall of the fixed step of slide and extend to the pin joint breach of the tip looks adaptation of the electrically conductive pin at sample slide edge outside.
As a further improvement, two the conductive electrode slice that all is equipped with on the inside wall of pin joint breach and contacts with conductive pin, two the conductive electrode slice is connected with the positive output and the negative pole output of power respectively.
As a further improvement of the utility model, two one end of the conductive electrode slice is extended to the table top of the slide fixing platform and is extended to the power-on connector connected with the power supply is arranged on the end part of the conductive electrode slice on the table top of the slide fixing platform.
As a further improvement of the present invention, the sectional area of the upper portion of the sample tank is smaller than that of the lower portion thereof; the cross section of the upper part of the sample groove is rectangular, and the cross section of the lower part of the sample groove is circular.
Advantageous effects
Compared with the prior art, the utility model discloses a device for carrying out magnetic field stimulation and electric field stimulation to cell's advantage does:
1. in the device, including ability axial pivoted U type electro-magnet, U type electro-magnet both ends are equipped with conductive pin respectively, can connect constant current, pulse current, can form constant magnetic field in sample groove place space, rotating magnetic field, pulse magnetic field etc, still be equipped with slide fixed platform between two magnetic poles of U type electro-magnet, the last removable sample slide of installing of slide fixed platform, sample slide upper surface is equipped with two conductive pin, still be equipped with many sample grooves between two conductive pin, sample groove bottom is equipped with the busbar, the busbar both ends are connected with two conductive pin respectively. In the device, the sample slide is of a detachable structure, because the size and the distance of the sample grooves on the sample slide are set according to actual experiment requirements, namely, a plurality of sample slides with different sample groove specifications are prepared, and the sample slide with the proper specification is selected to be installed on the slide fixing platform before the experiment. When the kit is used, a matrix material or animal tissue for culturing cells to be observed is placed in a sample groove in advance together with a culture solution, and a plurality of sample grooves form a control group. Then, the power supply discharges, and current passes through the conductive pins and the conductive strips to generate electric field stimulation on the biological materials in the culture solution or cells on the animal tissues. At the moment, the slide fixing platform is positioned between the two magnetic poles of the U-shaped electromagnet, so that cells to be observed on the sample slide can be stimulated by a magnetic field. Then, the device is placed under the microscope lens upside down, and the short-term or long-term growth condition of cells on the biological material or animal tissue can be observed by using the microscope, so that the cell behaviors such as adhesion, spreading, proliferation, differentiation and the like of the cells under the conditions of magnetic field stimulation and electric field stimulation can be observed. Through the device, the staff can be very convenient carry out the experiment of magnetic field stimulation and electric field stimulation condition to the cell of cultivateing on biomaterial or the animal tissue, convenient to use, and the observation result is accurate. Wherein, conductive pin is connected with the power, and the staff can also select suitable electric current, voltage through the mode of adjustment power output according to actual experiment needs to can also select DC power supply or alternating current power supply according to actual need, thereby can obtain various electric field stimulation environment, can form direct current electric field, sinusoidal electric field, pulse electric field in the culture solution of sample cell. Meanwhile, the U-shaped electromagnet can provide a common uniform constant magnetic field and is matched with the function of axial rotation of the U-shaped electromagnet, so that a uniform or non-uniform magnetic field with periodically changed N poles and S poles can be obtained. In addition, the staff can also turn off the power supply according to the needs, thereby observing the change of the cells only under the magnetic field or electric field stimulation environment, and observing the cell behaviors under the environment that the magnetic field and the electric field exist simultaneously.
2. One side of the slide fixing platform, which is far away from the U-shaped electromagnet, is connected with one end of a platform rotating shaft which is horizontally arranged and has the extending direction vertical to the extending direction of the sample groove, and the other end of the platform rotating shaft is in transmission connection with the power output end of a platform rotating motor. The lower part of the platform rotating motor is fixedly arranged on the workbench, and the workbench is provided with a motor controller connected with the platform rotating motor through a lead. Through the motor controller, the operation of the platform rotating motor can be accurately controlled, so that the inclination angle of the slide fixing platform is adjusted before an experiment, and observation is facilitated.
3. One side of the U-shaped electromagnet far away from the slide fixing platform is connected with one end of a magnet rotating shaft which is horizontally arranged and has an extending direction vertical to the extending direction of the sample groove, and the other end of the magnet rotating shaft is in transmission connection with the power output end of a magnet rotating motor. The lower part of the magnet rotating motor is fixedly arranged on the workbench, and the workbench is provided with a motor controller connected with the magnet rotating motor through a lead. Through the motor controller, the uniform rotation of the magnet rotating motor can be accurately controlled, so that a stable magnetic field is obtained, and meanwhile, the rotating speed is adjusted, so that the periodic variation parameters of the magnetic field in the experimental process are adjusted.
The invention will become more apparent from the following description when taken in conjunction with the accompanying drawings which illustrate embodiments of the invention.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a top view of the present invention;
FIG. 2 is a top view of the slide holding platform of the present invention with a sample slide mounted thereon;
FIG. 3 is a top view of the slide mounting platform of the present invention;
FIG. 4 is a front view of the slide retaining platform of the present invention;
FIG. 5 is a top view of a sample slide of the present invention;
FIG. 6 is a front cross-sectional view of a sample slide of the present invention;
fig. 7 is a front view of the magnet mounting plate of the present invention.
Wherein: 1-a workbench; 2-slide fixing platform; 21-mounting grooves; 22-slide fixing step; 221-pin clamping notches; 222-a conductive electrode sheet; 223-power-on connection; 23-a platform spindle; 3-a platform rotating motor; 4-a motor controller; 5-sample slide; 51-a sample cell; 52-conductive pins; 53-conductive strip; 6-U type electromagnet; 7-a magnet mounting plate; 71-a magnet fixing block; 72-magnet card slot; 73-locking bolts; 74-locking tab; 75-a magnet shaft; 8-magnet rotating machine.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings. It is to be understood that the embodiments described are only some embodiments of the invention, and not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Embodiments of the present invention will now be described with reference to the accompanying drawings.
Examples
This embodiment discloses a device for magnetic and electric field stimulation of cells, which comprises a horizontally arranged and axially rotatable U-shaped electromagnet 6, as shown in fig. 1-7. A slide fixing platform 2 is horizontally arranged between the two magnetic poles of the U-shaped electromagnet 6, and a sample slide 5 is detachably and horizontally arranged on the table surface of the slide fixing platform 2. Two conductive pins 52 which extend in the same direction with the magnetic poles of the U-shaped electromagnet 6 and one end of which is connected with a power supply are horizontally arranged on the upper surface of the sample slide 5. A plurality of sample grooves 51 which are vertical to the extending direction of the magnetic poles of the U-shaped electromagnet 6 are horizontally arranged on the upper surface of the sample slide 5 between the two conductive pins 52. The bottom of the sample groove 51 is provided with a conductive strip 53, and two ends of the conductive strip 53 extend out of the sample groove 51 and are respectively connected with the two conductive pins 52. The two ends of the U-shaped electromagnet 6 are respectively provided with a conductive pin 52, which can be connected with a constant current and a pulse current, and can form a constant magnetic field, a rotating magnetic field, a pulse magnetic field and the like in the space where the sample groove 51 is located. In the device, the sample slide 5 is a detachable structure, because the size and the distance of the sample grooves 51 on the sample slide 5 are set according to the actual experiment requirement, namely, a plurality of sample slides 5 with different sample groove 51 specifications are prepared, and the sample slide 5 with the proper specification is selected to be arranged on the slide fixing platform 2 before the experiment.
Regarding the observation experiment of cell magnetic field stimulation and electric field stimulation by using the device, the whole experiment process comprises the following steps:
s1: carrying out ultraviolet sterilization or ethylene oxide sterilization on matrix materials such as collagen, gelatin and the like or animal tissues;
s2: mixing cells including bone marrow mesenchymal stem cell, osteoblast, chondrocyte, cardiomyocyte, and nerve cell at 2-10 × 105Inoculating to biological material or animal tissue at a density of one ml, adding 10% fetal calf serum culture solution, adding double antibodies including penicillin and streptomycin at a concentration of 100 μ g/ml, and culturing in a cell culture box with carbon dioxide concentration of 5% and relative saturation humidity of 95%;
s3: placing a matrix material or animal tissue in which cells to be observed are cultured together with a culture solution into the sample wells 51, the plurality of sample wells 51 forming a control group;
s4: the power supply discharges, and the current generates electric field stimulation to the biological material in the culture solution or cells on the animal tissue through the conductive pins 52 and the conductive strips 53; meanwhile, the slide fixing platform 2 is positioned between two magnetic poles of the U-shaped electromagnet 6, so that the biological material in the culture solution or cells on animal tissues can generate magnetic field stimulation;
s5: the device is placed under a microscope lens in an inverted mode, and the short-term or long-term growth condition of cells on biological materials or animal tissues is observed by using the microscope, so that cell behaviors such as adhesion, spreading, proliferation and differentiation of the cells under the conditions of magnetic field stimulation, magnetic field stimulation and electric field stimulation are observed.
It should be noted that, in the steps S2-S5 of the whole experiment, 10% concentration of fetal bovine serum culture solution is added periodically-once every 1 day or once every 2 days.
Through the device, the staff can be very convenient carry out the experiment of magnetic field stimulation and electric field stimulation condition to the cell of cultivateing on biomaterial or the animal tissue, convenient to use, and the observation result is accurate. The conductive pins 52 are connected with a power supply, and the staff can select proper current and voltage by adjusting the power supply output mode according to actual experimental requirements, and can select a direct current power supply or an alternating current power supply according to actual requirements, so that various electric field stimulation environments can be obtained, and a direct current electric field, a sinusoidal electric field and a pulse electric field can be formed in the culture solution of the sample tank 51. Meanwhile, the U-shaped electromagnet 6 can provide a common uniform constant magnetic field, or according to the requirement, the two ends of the U-shaped electromagnet 6 are respectively provided with the conductive pins 52, which can be connected with constant current and pulse current, and can form a constant magnetic field, a rotating magnetic field, a pulse magnetic field and the like in the space where the sample cell is located, and the function that the constant magnetic field, the rotating magnetic field, the pulse magnetic field and the like can axially rotate is matched, so that a uniform or non-uniform magnetic field with periodically changed N pole and S pole can be obtained. In addition, the staff can also turn off the power supply according to the needs, thereby observing the change of the cells only under the magnetic field or electric field stimulation environment, and observing the cell behaviors under the environment that the magnetic field and the electric field exist simultaneously.
Wherein, one side of the slide fixing platform 2 far away from the U-shaped electromagnet 6 is connected with one end of a platform rotating shaft 23 which is horizontally arranged and the extending direction of which is vertical to the extending direction of the sample groove 51. The other end of the platform rotating shaft 23 is in transmission connection with the power output end of the platform rotating motor 3. The lower part of the platform rotating motor 3 is fixedly arranged on the workbench 1, and the workbench 1 is provided with a motor controller 4 connected with the platform rotating motor 3 through a lead. Through motor controller 4, can accurate control platform rotating electrical machines 3's operation to the inclination of slide fixed platform 2 has been adjusted before the experiment, and then the convenient observation. In this embodiment, the stage rotating motor 3 is a stepping motor.
One side of the U-shaped electromagnet 6 far away from the slide fixing platform 2 is connected with one end of a magnet rotating shaft 75 which is horizontally arranged and the extending direction of which is vertical to the extending direction of the sample groove 51, and the other end of the magnet rotating shaft 75 is in transmission connection with the power output end of the magnet rotating motor 8. The lower part of the magnet rotating motor 8 is fixedly arranged on the workbench 1, and the workbench 1 is provided with a motor controller 4 connected with the magnet rotating motor 8 through a lead. Through the motor controller, 4 can accurate control magnet rotating electrical machines 8 at the uniform velocity rotate to obtain a stable magnetic field, simultaneously, the adjustment rotational speed, and then the periodic variation parameter in adjustment experiment in-process magnetic field.
In this embodiment, a vertically arranged magnet fixing block 71 for connecting the magnet rotating shaft 75 and the U-shaped electromagnet 6 is disposed at the joint of the two. The magnet fixing block 71 is arranged on the plate surface of the magnet fixing block 71 close to one side of the U-shaped electromagnet 6. The middle part of the magnet fixing block 71 is provided with a magnet clamping groove 72. The upper part of the magnet fixing block 71 is also provided with a locking bolt 73 which is vertically arranged, the lower end of the locking bolt extends to the inner side of the magnet clamping groove 72, and the locking bolt 73 is provided with a locking sheet 74 on the end surface which is in contact with the U-shaped electromagnet 6.
Meanwhile, the table top of the slide fixing platform 2 below the sample slide 5 is horizontally provided with a mounting groove 21 penetrating through the whole table top, and the extending direction of the mounting groove 21 is vertical to the extending direction of the conductive pin 52. In this embodiment, the bottom surface of the mounting groove 21 is a cambered surface, and by using the mounting groove 21, the worker can conveniently load and take the sample slide 5.
And, the upper portion of both sides lateral wall of the mounting groove 21 has fixed step 22 of slide that fits with both sides edge of the sample slide 5, the sample slide 5 can pass this fixed step 22 of slide, the stable installation on slide fixed platform 2.
With respect to how the conductive pins 52 are connected to the power supply, in the present embodiment, the ends of the two conductive pins 52 connected to the power supply extend to the outside of the edge of the sample slide 5. The inner side wall of the slide fixing step 22 is provided with a pin clamping notch 221 matched with the end part of the conductive pin 52 extending to the outer part of the edge of the sample slide 5. The inner side walls of the two pin clamping notches 221 are provided with conductive electrode plates 222 contacted with the conductive pins 52, and the two conductive electrode plates 222 are respectively connected with the positive output end and the negative output end of the power supply.
Specifically, one end of each of the two conductive electrode pads 222 extends to the top of the slide holding platform 2, and an electrical connector 223 connected to a power supply is disposed at the end of the conductive electrode pad 222 extending to the top of the slide holding platform 2.
The cross-sectional area of the upper portion of the sample well 51 is smaller than that of the lower portion thereof in order to prevent the culture solution in the sample well 51 from flowing out when the sample well 51 is tilted. In this embodiment, the sample well 51 has a rectangular upper cross-sectional shape, and the sample well 51 has a circular lower cross-sectional shape.
The present invention has been described above with reference to the preferred embodiments, but the present invention is not limited to the above-disclosed embodiments, and various modifications, equivalent combinations, which are made according to the essence of the present invention, should be covered.
Claims (10)
1. A device for magnetic field stimulation and electric field stimulation to cells is characterized by comprising a U-shaped electromagnet (6) which is horizontally arranged and can axially rotate, a slide fixing platform (2) is horizontally arranged between the two magnetic poles of the U-shaped electromagnet (6), a sample slide (5) is detachably and horizontally arranged on the table surface of the slide fixing platform (2), two conductive pins (52) which extend in the same direction with the magnetic poles of the U-shaped electromagnet (6) and one end of which is connected with a power supply are horizontally arranged on the upper surface of the sample slide (5), a plurality of sample grooves (51) which are vertical to the extending direction of the magnetic poles of the U-shaped electromagnet (6) are also horizontally arranged on the upper surface of the sample slide (5) between the two conductive pins (52), and the bottom of the sample groove (51) is provided with a conductive strip (53), and two ends of the conductive strip (53) extend out of the sample groove (51) and are respectively connected with the two conductive pins (52).
2. The device for magnetic field stimulation and electric field stimulation of cells according to claim 1, wherein one side of the slide fixing platform (2) far away from the U-shaped electromagnet (6) is connected with one end of a platform rotating shaft (23) which is horizontally arranged and has an extending direction perpendicular to the extending direction of the sample slot (51), and the other end of the platform rotating shaft (23) is in transmission connection with the power output end of the platform rotating motor (3); the lower part of the platform rotating motor (3) is fixedly arranged on the workbench (1), and a motor controller (4) connected with the platform rotating motor (3) through a wire is arranged on the workbench (1).
3. The device for magnetic field stimulation and electric field stimulation of cells according to claim 1 or 2, characterized in that one side of the U-shaped electromagnet (6) far away from the slide fixing platform (2) is connected with one end of a magnet rotating shaft (75) which is horizontally arranged and the extending direction of the magnet rotating shaft is vertical to the extending direction of the sample groove (51), and the other end of the magnet rotating shaft (75) is in transmission connection with the power output end of a magnet rotating motor (8); the lower part of the magnet rotating motor (8) is fixedly arranged on the workbench (1), and a motor controller (4) connected with the magnet rotating motor (8) through a wire is arranged on the workbench (1).
4. The device for magnetic field stimulation and electric field stimulation of cells according to claim 3, wherein a vertically arranged magnet fixing block (71) for connecting the magnet rotating shaft (75) and the U-shaped electromagnet (6) is arranged at the joint of the magnet rotating shaft (75) and the U-shaped electromagnet (6), the magnet fixing block (71) is arranged on the surface of one side, close to the U-shaped electromagnet (6), of the magnet fixing block (71), a magnet clamping groove (72) is formed in the middle of the magnet fixing block (71), a locking bolt (73) which is vertically arranged and of which the lower end extends to the inner side of the magnet clamping groove (72) is further arranged on the upper portion of the magnet fixing block (71), and a locking sheet (74) is arranged on the end face, which is in contact with the U-shaped electromagnet (6), of the locking bolt (73).
5. The device for magnetic field stimulation and electric field stimulation to the cells is characterized in that a mounting groove (21) penetrating through the whole table top is horizontally arranged on the table top of the slide fixing platform (2) below the sample slide (5), and the extending direction of the mounting groove (21) is perpendicular to the extending direction of the conductive pins (52).
6. The device for magnetic and electric field stimulation of cells according to claim 5, wherein the upper parts of both side walls of the mounting groove (21) are provided with slide fixing steps (22) adapted to both side edges of the sample slide (5).
7. The device for magnetic field stimulation and electric field stimulation of cells according to claim 6, wherein one end of the two conductive pins (52) connected with the power supply extends to the outside of the edge of the sample slide (5), and the inner side wall of the slide fixing step (22) is provided with a pin clamping notch (221) matched with the end of the conductive pin (52) extending to the outside of the edge of the sample slide (5).
8. The device for magnetic field stimulation and electric field stimulation of cells according to claim 7, wherein the inner side walls of the two pin clamping notches (221) are provided with conductive electrode pieces (222) contacting with the conductive pins (52), and the two conductive electrode pieces (222) are respectively connected with the positive output end and the negative output end of the power supply.
9. The device for magnetic field stimulation and electric field stimulation of cells according to claim 8, wherein one end of each of the two conductive electrode sheets (222) extends to the top of the slide holding platform (2), and the end of the conductive electrode sheet (222) extending to the top of the slide holding platform (2) is provided with an electric connector (223) connected with a power supply.
10. The device for magnetic and electric field stimulation of cells according to claim 1, wherein the cross-sectional area of the upper part of the sample well (51) is smaller than that of the lower part thereof; the cross-sectional shape of the upper part of the sample groove (51) is rectangular, and the cross-sectional shape of the lower part of the sample groove (51) is circular.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120973082.3U CN215250921U (en) | 2021-05-08 | 2021-05-08 | Device for magnetic field stimulation and electric field stimulation of cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120973082.3U CN215250921U (en) | 2021-05-08 | 2021-05-08 | Device for magnetic field stimulation and electric field stimulation of cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215250921U true CN215250921U (en) | 2021-12-21 |
Family
ID=79454402
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120973082.3U Active CN215250921U (en) | 2021-05-08 | 2021-05-08 | Device for magnetic field stimulation and electric field stimulation of cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215250921U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115029238A (en) * | 2022-06-08 | 2022-09-09 | 哈尔滨医科大学 | Experimental device and method for researching biological effect mechanism of pulsed magnetic field |
-
2021
- 2021-05-08 CN CN202120973082.3U patent/CN215250921U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115029238A (en) * | 2022-06-08 | 2022-09-09 | 哈尔滨医科大学 | Experimental device and method for researching biological effect mechanism of pulsed magnetic field |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mobini et al. | Direct current electrical stimulation chamber for treating cells in vitro | |
| Jaffe et al. | Neurites grow faster towards the cathode than the anode in a steady field | |
| Cohen et al. | Effect of low‐level, 60‐Hz electromagnetic fields on human lymphoid cells: I. Mitotic rate and chromosome breakage in human peripheral lymphocytes | |
| CN215250921U (en) | Device for magnetic field stimulation and electric field stimulation of cells | |
| Song et al. | Application of direct current electric fields to cells and tissues in vitro and modulation of wound electric field in vivo | |
| US6114164A (en) | System and method for emulating an in vivo environment of a muscle tissue specimen | |
| CN1427255A (en) | Apparatus for irritating animal cell and recording its physiological signal and its production and using method | |
| Mazzoleni et al. | Conductivity values of tissue culture medium from 20 C to 40 C | |
| JP2006296282A (en) | Method for preparing cultured myocyte having high metabolizing function | |
| Cannizzaro et al. | Practical aspects of cardiac tissue engineering with electrical stimulation | |
| CN214830404U (en) | Device for electrically stimulating cells | |
| CN214781905U (en) | Non-contact electric field device for cell culture | |
| Arai et al. | Pulsed electric current induces the differentiation of human keratinocytes | |
| Elliott et al. | Time‐varying magnetic fields: Effects of orientation on chondrocyte proliferation | |
| CN102337578A (en) | Double-sided plating tank, sheet and plating method | |
| CN110484442A (en) | Biological cell stimulating system and its control method based on annular spread electrode | |
| Dubey et al. | Pulsed electric field mediated in vitro cellular response of fibroblast and osteoblast-like cells on conducting austenitic stainless steel substrate | |
| CN212451451U (en) | Cell culture device with micro-current stimulation function | |
| US20130344559A1 (en) | Device and method for controlling nerve growth | |
| CN106318868B (en) | A kind of organizational project dynamic cultivation instrument stimulated using dynamic mechanical | |
| Soong et al. | Effects of electric fields on cytoskeleton of corneal stromal fibroblasts | |
| Noda et al. | Effect of electric currents on DNA synthesis in rat osteosarcoma cells: Dependence on conditions that influence cell growth | |
| JP2022544729A (en) | Cell culture device with microcurrent stimulation function | |
| Karanth et al. | Orthodontic tooth movement and the bioelectricity | |
| CN212426065U (en) | Electrical stimulation cell culture device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |