CN212451451U - Cell culture device with micro-current stimulation function - Google Patents
Cell culture device with micro-current stimulation function Download PDFInfo
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- CN212451451U CN212451451U CN202022128710.6U CN202022128710U CN212451451U CN 212451451 U CN212451451 U CN 212451451U CN 202022128710 U CN202022128710 U CN 202022128710U CN 212451451 U CN212451451 U CN 212451451U
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Abstract
A cell culture device with a micro-current stimulation function comprises a box body, an upper guide rail, an upper magnetic block, an upper sliding block, a lower guide rail, a lower magnetic block, a lower sliding block and an object stage; the box body is a medical grade polymer box body or a stainless steel box body; the box body comprises an incubator and a control box; the incubator and the control box are arranged side by side; the upper guide rail, the upper magnetic block, the upper sliding block, the lower guide rail, the lower magnetic block, the lower sliding block and the objective table are all arranged in the incubator; the object stage is positioned in the middle of the upper guide rail and the lower guide rail; the objective table is horizontally arranged; the upper sliding block is arranged on the upper guide rail in a sliding mode, and the upper magnetic block is arranged on the lower surface of the upper sliding block. The utility model discloses, through carry out the electro photoluminescence at multiple cell culture in-process, like tendon, ligament, nerve, blood vessel, skin, muscle and alimentary canal cell etc. especially can promote proliferation, differentiation and the growth of nerve cell, reach the beneficial effect who promotes neural restoration and regeneration.
Description
Technical Field
The utility model relates to a cell culture device field especially relates to a cell culture device with little current stimulation function.
Background
Peripheral nerve damage is a common clinical condition, and the repair and regeneration of the peripheral nerve damage are difficult problems which plague neurosurgery experts and material experts. When damaged for a long distance and a long time, the damaged nerve can not be directly sutured and repaired; autografts and allografts suffer from secondary injury, limited sources, cross-infection and xeno-rejection, and researchers have therefore begun research in artificial nerve grafts. In practical applications, the artificial nerve graft is usually co-cultured with nerve cells and then transplanted to the nerve defect site. Recent studies have shown that when nerve cells are stimulated by micro-current during culture, they are more favorable for proliferation, differentiation and growth, and thus can better promote repair and regeneration of damaged axonal nerves.
The in vitro culture of nerve cells by adopting the artificial electrical stimulation device is more beneficial to the tissue engineering of the nerve conduit. Currently, a conventional method for electrically stimulating cells uses a primary battery to generate a weak current by generating an electromotive force through a chemical reaction between an electrode and an electrolyte, and for example, chinese patent publication No. CN201920576816.7 provides a nerve electrical stimulation electrode assembly and a nerve electrical stimulation device to generate a weak current by using a chemical reaction between an electrode and an electrolyte in a primary battery. This manner of generating current through chemical reactions has the disadvantage that the rate of chemical reaction changes with the reaction process, which may cause changes in the current generated and require replacement of the electrolyte and electrodes over a period of time. In addition, the primary battery may generate side reactions, which results in the reduction of charging and discharging efficiency, capacity life loss and performance degradation of the battery, for example, Chinese patent (publication: CN92242285.0) provides a demonstration instrument for copper-zinc primary battery, which is composed of twin cylinders, salt bridges, copper sheets, zinc sheets, specially-made small bulbs, and positive and negative terminals, and the method uses zinc as the negative electrode of the primary battery. When pure zinc is used as an electrode, a small amount of bubbles are generated on the zinc sheet; when impure zinc is used as the electrode, more bubbles are generated on the zinc sheet. This is due to the fact that the zinc on the zinc sheet forms many tiny galvanic cells with surface impurities, and side reactions occur. Can not provide stable and continuous power supply for the electrical stimulation device, and is not beneficial to the normal culture of cells.
Therefore, how to solve the above-mentioned drawbacks needs to be solved, and how to provide a cell culture device having a continuously stable and safe and environmentally friendly electrical stimulation function.
SUMMERY OF THE UTILITY MODEL
Objects of the invention
For solving the technical problem that exists among the background art, the utility model provides a cell culture device with little current stimulation function promotes multiple cell better through the electro photoluminescence, like tendon, ligament, nerve, blood vessel, skin, muscle and alimentary canal cell etc. especially is favorable to the proliferation, differentiation and the growth of nerve cell to can promote neural restoration and regeneration, reach more ideal effect.
(II) technical scheme
In order to solve the problems, the utility model provides a cell culture device with a micro-current stimulation function, which comprises a box body, an upper guide rail, an upper magnetic block, an upper slide block, a lower guide rail, a lower magnetic block, a lower slide block and an object stage;
the box body is a medical grade polymer box body or a stainless steel box body; the box body comprises an incubator and a control box; the incubator and the control box are arranged side by side;
the upper guide rail, the upper magnetic block, the upper sliding block, the lower guide rail, the lower magnetic block, the lower sliding block and the objective table are all arranged in the incubator; the object stage is positioned in the middle of the upper guide rail and the lower guide rail; the objective table is horizontally arranged;
the upper sliding block is arranged on the upper guide rail in a sliding manner, and the upper magnetic block is arranged on the lower surface of the upper sliding block; the lower sliding block is arranged on the lower guide rail in a sliding manner, and the lower magnetic block is arranged on the upper surface of the lower sliding block; the magnetic poles of the upper magnetic block and the lower magnetic block are opposite to each other on the surface facing the objective table;
and a control device for controlling the sliding of the upper sliding block and the lower magnetic block is arranged in the control box.
Preferably, the distance between the object stage and the upper guide rail and the distance between the object stage and the lower guide rail are 1-50 cm; the distance between the bottom surface of the upper magnetic block and the objective table is X; the distance between the upper surface of the lower magnetic block and the objective table is X; x is 0.5-50 cm.
Preferably, the sliding speed of the upper slide block and the lower slide block is 1-100 cm/s.
Preferably, two ends of the upper guide rail and the lower guide rail are respectively an M end and an N end;
in the initial state: the upper sliding block is arranged at the position of the M end of the upper guide rail in a sliding manner, and the lower sliding block is arranged at the position of the M end of the lower guide rail in a sliding manner;
under the motion state, the upper slide block and the lower slide block synchronously move along the reciprocating motion from M to N and then from N to M; the moving directions and the moving speeds of the upper sliding block and the lower sliding block are always the same;
the conducting device with the length of L and the culture dish for spreading the cells are placed at the central position of the objective table;
the moving speed of the upper slide block and the lower slide block is V; the conductive device cuts the magnetic induction line, and the calculation formula of the current I1 is as follows:
I1＝B×L×V×Sinα/R；
alpha is an included angle between the conductive device and the vertical magnetic induction line;
r is the resistance of the conductive device;
b is the magnetic induction intensity between the upper magnetic block and the lower magnetic block;
the conducting device is a metal wire or a metal pipe or a metal rod.
Preferably, two ends of the upper guide rail and the lower guide rail are respectively an M end and an N end;
in the initial state: the upper sliding block is arranged at the position of the M end of the upper guide rail in a sliding manner, and the lower sliding block is arranged at the position of the N end of the lower guide rail in a sliding manner;
in the motion state, the upper sliding block reciprocates from M to N and then from N to M; the lower sliding block reciprocates from N to M and then from M to N;
the moving directions of the upper sliding block and the lower sliding block are always opposite, and the moving speeds are always the same;
the conducting device with the length of L and the culture dish for spreading the cells are placed at the central position of the objective table;
the moving speed of the upper slide block and the lower slide block is V; the conductive device cuts the magnetic induction line, and the calculation formula of the current I2 is as follows:
I2＝B×L×V×Sinα/R；
alpha is an included angle between the conductive device and the vertical magnetic induction line;
r is the resistance of the conductive device;
b is the magnetic induction intensity between the upper magnetic block and the lower magnetic block;
the conducting device is a metal wire or a metal pipe or a metal rod.
Preferably, two ends of the upper guide rail and the lower guide rail are respectively an M end and an N end;
in the initial state: the lower sliding block is fixedly arranged at the position of the M end or the position of the N end of the lower guide rail;
in the motion state, the upper sliding block reciprocates from M to N and then from N to M; the lower sliding block is always fixed;
the conducting device with the length of L and the culture dish for spreading the cells are placed at the central position of the objective table; the moving speed of the upper sliding block is V; the conductive device cuts the magnetic induction line, and the calculation formula of the current I3 is as follows:
I3＝B×L×V×Sinα/R；
alpha is an included angle between the conductive device and the vertical magnetic induction line;
r is the resistance of the conductive device;
b is the magnetic induction intensity between the upper magnetic block and the lower magnetic block;
the conducting device is a metal wire or a metal pipe or a metal rod.
The utility model discloses, promote multiple cell better through the electro photoluminescence, like tendon, ligament, nerve, blood vessel, skin, muscle and alimentary canal cell etc. especially nerve cell's proliferation, differentiation and growth to can promote neural restoration and regeneration, reach more ideal effect.
The device has simple structure, low production and maintenance cost and easy industrial popularization;
the utility model discloses, set for direction of motion, orbit, the speed of going up magnetic path and lower magnetic path in order to change magnetic field through the procedure to realize the automated control of cell culture process.
The utility model discloses, according to "magnetism generates electricity" principle, can maintain the stable change in magnetic field, can produce stable electric current, need not to use galvanic cell, need not to use electrolyte and electrode, and it is continuously stable, green.
Drawings
FIG. 1 is a schematic structural diagram of a cell culture device with micro-current stimulation according to the present invention.
FIG. 2 is a schematic view of a first embodiment of the cell culture apparatus with micro-current stimulation according to the present invention.
FIG. 3 is a schematic view of a cell culture apparatus with micro-current stimulation according to a second embodiment of the present invention.
FIG. 4 is a schematic view of a third embodiment of the cell culture apparatus with micro-current stimulation according to the present invention.
Fig. 5 shows that the growth state of the nerve cells cultured in the cell culture device with micro-current stimulation function for three days is good, which illustrates that the device provided by the invention is beneficial to the growth and proliferation of the nerve cells.
FIG. 6 shows the growth state of nerve cells after three days of culture in a control normal environment. The growth state of the cells cultured in the common environment is poorer than that of the cells cultured in the same time in the device of the utility model, which shows that the device provided by the invention is beneficial to the culture of nerve cells.
FIG. 7 is a diagram showing the change of the instantaneous electromotive force of 10 magnesium wires with a length of 10cm in the cell culture device with micro-current stimulation according to the present invention over time. When the magnets of the upper and lower guide rails move linearly in the same direction at a constant speed, the instantaneous electromotive force generated when the magnesium wire cuts the magnetic induction wire is about 0.35V for about 4 seconds, and the electromotive force is changed to 0.1V at the moment of changing the direction. The change is relatively regular as can be seen from the figure, and is suitable for the theoretical calculation formula provided by the invention. The device provided by the invention is proved to be capable of generating micro-current which is beneficial to the culture of nerve cells.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in detail with reference to the accompanying drawings. It should be understood that the description is intended to be illustrative only and is not intended to limit the scope of the present invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.
As shown in fig. 1-7, the cell culture apparatus with micro-current stimulation function provided by the present invention comprises a box 4, an upper guide rail 11, an upper magnetic block 21, an upper sliding block 31, a lower guide rail 12, a lower magnetic block 22, a lower sliding block 32 and an object stage 6;
the box body 4 is a medical grade polymer box body or a stainless steel box body; the box body 4 comprises an incubator 41 and a control box 42; the incubator 41 and the control box 42 are arranged side by side;
the upper guide rail 11, the upper magnet 21, the upper slide block 31, the lower guide rail 12, the lower magnet 22, the lower slide block 32 and the objective table 6 are all arranged in the incubator 41; the object stage 6 is positioned at the middle position between the upper guide rail 11 and the lower guide rail 12; the objective table 6 is horizontally arranged;
the upper sliding block 31 is arranged on the upper guide rail 11 in a sliding manner, and the upper magnetic block 21 is arranged on the lower surface of the upper sliding block 31; the lower sliding block 32 is arranged on the lower guide rail 12 in a sliding manner, and the lower magnetic block 22 is arranged on the upper surface of the lower sliding block 32; the magnetic poles of the upper magnetic block 21 and the lower magnetic block 22 are opposite to each other on the surface facing the objective table 6;
the control box 42 is provided with a control device for controlling the sliding of the upper slider 31 and the lower magnetic block 22.
In an alternative embodiment, the distance between the object table 6 and the upper rail 11 and the lower rail 12 is 1-50 cm;
the distance between the bottom surface of the upper magnetic block 21 and the objective table 6 is X; the distance between the upper surface of the lower magnetic block 22 and the objective table 6 is X; x is 0.5-50 cm.
In an alternative embodiment, the upper slide 31 and the lower slide 32 slide at a speed of 1-100 cm/s.
The utility model discloses in, according to "magnetism generates electricity" principle, when cutting magnetic induction line motion is done to a part conductor of closed circuit, just can produce induced-current on the conductor. Under the magnetic field environment, only the conductive materials (such as medical human body pipelines, metal wires and the like) are placed around the cells, and when the magnetic field is changed, the purpose of automatically cutting the magnetic induction lines can be achieved, so that induced current is generated, the cells receive electrical stimulation, and the proliferation, differentiation and growth of the cells are better promoted. Compared with the method of the primary battery, the device based on the principle of magnetic electricity generation has no chemical reaction, so that the current generated by the change of the chemical reaction rate does not change, and the trouble that the electrolyte and the electrode need to be replaced is avoided.
In the utility model, the reciprocating motion of the cell culture dish or the magnetic device cuts the magnetic field uniformly, so as to generate stable current, and the device is particularly suitable for a damaged nerve regeneration promoting device based on the 'magnetoelectric' principle; placing a culture dish with nerve cells and a conductive device between an upper magnetic block 21 and a lower magnetic block 22, changing a magnetic field through the movement of the upper magnetic block 21 and the lower magnetic block 22, and enabling the conductive device to automatically cut magnetic induction lines; furthermore, induced current is generated around the conducting device by changing the magnetic field, so that nerve cells receive electric stimulation, and the nerve cells are better cultured, thereby achieving the purpose of promoting the repair and regeneration of damaged nerves.
The utility model discloses, through carry out the electro photoluminescence at multiple cell culture in-process, like tendon, ligament, nerve, blood vessel, skin, muscle and alimentary canal cell etc. especially can promote proliferation, differentiation and the growth of nerve cell, reach the beneficial effect who promotes neural restoration and regeneration.
Example 1
In the utility model, the two ends of the upper guide rail 11 and the lower guide rail 12 are respectively an M end and an N end;
in the initial state: the upper slide block 31 is arranged at the position of M end of the upper guide rail 11 in a sliding way, and the lower slide block 32 is arranged at the position of M end of the lower guide rail 12 in a sliding way;
in the motion state, the upper slide block 31 and the lower slide block 32 move synchronously and reciprocate from M to N and then from N to M; the moving directions and the moving speeds of the upper slider 31 and the lower slider 32 are always the same;
the conducting device with the length L and the culture dish for spreading the cells are placed at the central position of the objective table 6;
the moving speed of the upper slider 31 and the lower slider 32 is V; the conductive device cuts the magnetic induction line, and the calculation formula of the current I1 is as follows:
I1＝B×L×V×Sinα/R；
alpha is an included angle between the conductive device and the vertical magnetic induction line;
r is the resistance of the conductive device;
b is the magnetic induction intensity between the upper magnetic block 21 and the lower magnetic block 22;
the conducting device is a metal wire or a metal pipe or a metal rod.
It should be noted that the current acts to stimulate the growth of nerve cells around the catheter, thereby accelerating the regeneration and repair of the damaged tissue.
Example 2
In the utility model, the two ends of the upper guide rail 11 and the lower guide rail 12 are respectively an M end and an N end;
in the initial state: the upper slide block 31 is arranged at the position of M end of the upper guide rail 11 in a sliding way, and the lower slide block 32 is arranged at the position of N end of the lower guide rail 12 in a sliding way;
in the moving state, the upper slide block 31 reciprocates from M to N and then from N to M; the lower slide block 32 reciprocates from N to M and then from M to N;
the moving directions of the upper sliding block 31 and the lower sliding block 32 are always opposite, and the moving speeds are always the same;
the conducting device with the length L and the culture dish for spreading the cells are placed at the central position of the objective table 6;
the moving speed of the upper slider 31 and the lower slider 32 is V; the conductive device cuts the magnetic induction line, and the calculation formula of the current I2 is as follows:
I2＝B×L×V×Sinα/R；
alpha is an included angle between the conductive device and the vertical magnetic induction line;
r is the resistance of the conductive device;
b is the magnetic induction intensity between the upper magnetic block 21 and the lower magnetic block 22;
the conducting device is a metal wire or a metal pipe or a metal rod.
It should be noted that the action of the current can stimulate damaged axonal nerve cells around the catheter, promote the proliferation, differentiation and growth of damaged axonal nerve cells, and accelerate the regeneration and repair of damaged axonal nerves.
Example 3
In the utility model, the two ends of the upper guide rail 11 and the lower guide rail 12 are respectively an M end and an N end;
in the initial state: the lower slide block 32 is fixedly arranged at the position of M end or the position of N end of the lower guide rail 12;
in the moving state, the upper slide block 31 reciprocates from M to N and then from N to M; the lower slider 32 is always stationary;
the conducting device with the length L and the culture dish for spreading the cells are placed at the central position of the objective table 6; the moving speed of the upper slider 31 is V; the conductive device cuts the magnetic induction line, and the calculation formula of the current I3 is as follows:
I3＝B×L×V×Sinα/R；
alpha is an included angle between the conductive device and the vertical magnetic induction line;
r is the resistance of the conductive device;
b is the magnetic induction intensity between the upper magnetic block 21 and the lower magnetic block 22;
the conducting device is a metal wire or a metal pipe or a metal rod.
It should be noted that the action of the current can stimulate the growth of the nerve cells around the catheter to a certain extent, so that the damaged peripheral nerves at long distance and for a long time are accelerated to regenerate and repair, and the nerve cells can be cultured in vitro to promote the proliferation, differentiation and growth of the nerve cells.
To sum up, the utility model has the advantages of it is following:
the utility model can better promote various cells such as tendon, ligament, nerve, blood vessel, skin, muscle and digestive tract cell, especially the proliferation, differentiation and growth of nerve cell by electrical stimulation, thereby promoting nerve repair and regeneration and achieving more ideal effect;
the device has simple structure, low production and maintenance cost and easy industrial popularization;
the utility model sets the movement tracks of the upper magnetic block 21 and the lower magnetic block 22 by a program to change the magnetic field and realize the automatic control of the cell culture process;
the utility model discloses, according to "magnetism generates electricity" principle, can maintain the stable change in magnetic field, can produce stable electric current, need not to use galvanic cell, need not to use electrolyte and electrode, green.
It is to be understood that the above-described embodiments of the present invention are merely illustrative of or explaining the principles of the invention and are not to be construed as limiting the invention. Therefore, any modification, equivalent replacement, improvement and the like made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. Further, it is intended that the appended claims cover all such variations and modifications as fall within the scope and boundaries of the appended claims or the equivalents of such scope and boundaries.
Claims (6)
1. A cell culture device with a micro-current stimulation function is characterized by comprising a box body (4), an upper guide rail (11), an upper magnetic block (21), an upper sliding block (31), a lower guide rail (12), a lower magnetic block (22), a lower sliding block (32) and an object stage (6);
the box body (4) is a medical grade polymer box body or a stainless steel box body; the box body (4) comprises an incubator (41) and a control box (42); the incubator (41) and the control box (42) are arranged side by side;
the upper guide rail (11), the upper magnetic block (21), the upper sliding block (31), the lower guide rail (12), the lower magnetic block (22), the lower sliding block (32) and the objective table (6) are all arranged in the incubator (41); the objective table (6) is positioned at the middle position of the upper guide rail (11) and the lower guide rail (12); the objective table (6) is horizontally arranged;
the upper sliding block (31) is arranged on the upper guide rail (11) in a sliding mode, and the upper magnetic block (21) is arranged on the lower surface of the upper sliding block (31); the lower sliding block (32) is arranged on the lower guide rail (12) in a sliding mode, and the lower magnetic block (22) is arranged on the upper surface of the lower sliding block (32); the upper magnetic block (21) and the lower magnetic block (22) face one side of the objective table (6), and the magnetic poles of the upper magnetic block (21) and the lower magnetic block (22) are opposite;
the control box (42) is internally provided with a control device for controlling the sliding of the upper sliding block (31) and the lower magnetic block (22).
2. The cell culture apparatus with micro-current stimulation according to claim 1, wherein the distance between the stage (6) and the upper rail (11) and the lower rail (12) is 1-50 cm;
the distance between the bottom surface of the upper magnetic block (21) and the objective table (6) is X; the distance between the upper surface of the lower magnetic block (22) and the objective table (6) is X; x is 0.5-50 cm.
3. The cell culture apparatus with micro-current stimulation according to claim 1, wherein the sliding speed of the upper slider (31) and the lower slider (32) is 1-100 cm/s.
4. The cell culture apparatus with micro-current stimulation according to any one of claims 1 to 3, wherein the two ends of the upper rail (11) and the lower rail (12) are M-end and N-end, respectively;
in the initial state: the upper slide block (31) is arranged at the position of the M end of the upper guide rail (11) in a sliding manner, and the lower slide block (32) is arranged at the position of the M end of the lower guide rail (12) in a sliding manner;
in the motion state, the upper sliding block (31) and the lower sliding block (32) synchronously move along the reciprocating motion from M to N and then from N to M; the moving directions and the moving speeds of the upper sliding block (31) and the lower sliding block (32) are always the same;
the conducting device with the length L and the culture dish for spreading the cells are placed at the central position of the objective table (6);
the moving speed of the upper slide block (31) and the lower slide block (32) is V; the conductive device cuts the magnetic induction line, and the calculation formula of the current I1 is as follows:
I1＝(B×L×V×Sinα)/R；
alpha is an included angle between the conductive device and the vertical magnetic induction line;
r is the resistance of the conductive device;
b is the magnetic induction intensity between the upper magnetic block (21) and the lower magnetic block (22);
the conducting device is a metal wire or a metal pipe or a metal rod.
5. The cell culture apparatus with micro-current stimulation according to any one of claims 1 to 3, wherein the two ends of the upper rail (11) and the lower rail (12) are M-end and N-end, respectively;
in the initial state: the upper slide block (31) is arranged at the position of the M end of the upper guide rail (11) in a sliding manner, and the lower slide block (32) is arranged at the position of the N end of the lower guide rail (12) in a sliding manner;
in the motion state, the upper sliding block (31) reciprocates from M to N and then from N to M; the lower sliding block (32) reciprocates from N to M and then from M to N;
the moving directions of the upper sliding block (31) and the lower sliding block (32) are always opposite, and the moving speeds are always the same;
the conducting device with the length L and the culture dish for spreading the cells are placed at the central position of the objective table (6);
the moving speed of the upper slide block (31) and the lower slide block (32) is V; the conductive device cuts the magnetic induction line, and the calculation formula of the current I2 is as follows:
I2＝(B×L×V×Sinα)/R；
alpha is an included angle between the conductive device and the vertical magnetic induction line;
r is the resistance of the conductive device;
b is the magnetic induction intensity between the upper magnetic block (21) and the lower magnetic block (22);
the conducting device is a metal wire or a metal pipe or a metal rod.
6. The cell culture apparatus with micro-current stimulation according to any one of claims 1 to 3, wherein the two ends of the upper rail (11) and the lower rail (12) are M-end and N-end, respectively;
in the initial state: the lower sliding block (32) is fixedly arranged at the position of M end or the position of N end of the lower guide rail (12);
in the motion state, the upper sliding block (31) reciprocates from M to N and then from N to M; the lower sliding block (32) is always fixed;
the conducting device with the length L and the culture dish for spreading the cells are placed at the central position of the objective table (6); the moving speed of the upper sliding block (31) is V; the conductive device cuts the magnetic induction line, and the calculation formula of the current I3 is as follows:
I3＝(B×L×V×Sinα)/R；
alpha is an included angle between the conductive device and the vertical magnetic induction line;
r is the resistance of the conductive device;
b is the magnetic induction intensity between the upper magnetic block (21) and the lower magnetic block (22);
the conducting device is a metal wire or a metal pipe or a metal rod.
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| WO2022062376A1 (en) * | 2020-09-25 | 2022-03-31 | 南通纺织丝绸产业技术研究院 | Cell culture device with micro-current stimulation function |
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| WO2022062376A1 (en) * | 2020-09-25 | 2022-03-31 | 南通纺织丝绸产业技术研究院 | Cell culture device with micro-current stimulation function |
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