CN115023502A - 染色质的3d组织和转录活动的基因组规模成像 - Google Patents

染色质的3d组织和转录活动的基因组规模成像 Download PDF

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CN115023502A
CN115023502A CN202080087114.8A CN202080087114A CN115023502A CN 115023502 A CN115023502 A CN 115023502A CN 202080087114 A CN202080087114 A CN 202080087114A CN 115023502 A CN115023502 A CN 115023502A
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nucleic acid
sample
determining
probes
exposing
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庄小威
B·宾图
S·S·金若特
郑璞
苏俊翰
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Harvard University
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
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    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/10—Signal processing, e.g. from mass spectrometry [MS] or from PCR
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    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional [2D] or three-dimensional [3D] molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/10—Nucleic acid folding
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional [2D] or three-dimensional [3D] molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/30—Drug targeting using structural data; Docking or binding prediction

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080087114.8A 2019-12-30 2020-12-18 染色质的3d组织和转录活动的基因组规模成像 Pending CN115023502A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962954720P 2019-12-30 2019-12-30
US62/954,720 2019-12-30
US202063060947P 2020-08-04 2020-08-04
US63/060,947 2020-08-04
PCT/US2020/065797 WO2021138078A1 (en) 2019-12-30 2020-12-18 Genome-scale imaging of the 3d organization and transcriptional activity of chromatin

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CN115023502A true CN115023502A (zh) 2022-09-06

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CN202080087114.8A Pending CN115023502A (zh) 2019-12-30 2020-12-18 染色质的3d组织和转录活动的基因组规模成像

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US (1) US20230348958A1 (https=)
EP (1) EP4085150A4 (https=)
JP (1) JP7849034B2 (https=)
CN (1) CN115023502A (https=)
AU (1) AU2020418497A1 (https=)
CA (1) CA3161593A1 (https=)
WO (1) WO2021138078A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116052759A (zh) * 2022-12-09 2023-05-02 合肥本源量子计算科技有限责任公司 一种哈密顿量构造方法及相关装置

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* Cited by examiner, † Cited by third party
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EP4328322A3 (en) 2014-07-30 2024-05-22 President and Fellows of Harvard College Probe library construction
EP3894583A4 (en) 2018-12-13 2022-11-09 President and Fellows of Harvard College AMPLIFICATION METHODS AND SYSTEMS FOR MERFISH AND OTHER APPLICATIONS
WO2023097244A1 (en) * 2021-11-24 2023-06-01 Yale University Methods of determining chromatin alterations
WO2023122041A1 (en) * 2021-12-20 2023-06-29 Enumerix, Inc. Detection and digital quantitation of multiple targets
EP4453246A4 (en) 2021-12-20 2025-12-24 Countable Labs Inc DIGITAL DETECTION AND QUANTIFICATION OF MULTIPLE TARGETS
US20240158836A1 (en) * 2022-06-30 2024-05-16 Resolve Biosciences Gmbh High resolution multiplex method for detecting at least two targets with a distance of beyond the diffraction limit in a sample
CN120721467B (zh) * 2025-08-29 2026-04-03 华中科技大学同济医学院附属协和医院 一种用于定位检测新生rna合成水平的多重免疫荧光染色方法及试剂盒

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106715768A (zh) * 2014-07-30 2017-05-24 哈佛学院院长及董事 用于测定核酸的系统和方法
CN107208144A (zh) * 2014-11-21 2017-09-26 纳米线科技公司 无酶且无扩增的测序
CN113366116A (zh) * 2018-12-13 2021-09-07 哈佛学院院长及董事 用于merfish和其他应用的扩增方法和系统

Family Cites Families (1)

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Publication number Priority date Publication date Assignee Title
EP3539036A4 (en) * 2016-11-08 2020-06-17 President and Fellows of Harvard College MATRIX AND DELETE

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106715768A (zh) * 2014-07-30 2017-05-24 哈佛学院院长及董事 用于测定核酸的系统和方法
CN107208144A (zh) * 2014-11-21 2017-09-26 纳米线科技公司 无酶且无扩增的测序
CN113366116A (zh) * 2018-12-13 2021-09-07 哈佛学院院长及董事 用于merfish和其他应用的扩增方法和系统

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BOGDAN BINTU等: "Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells", SCIENCE, vol. 362, no. 6413, 26 October 2018 (2018-10-26), pages 1 - 8, XP055838244, DOI: 10.1126/science.aau1783 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116052759A (zh) * 2022-12-09 2023-05-02 合肥本源量子计算科技有限责任公司 一种哈密顿量构造方法及相关装置

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JP7849034B2 (ja) 2026-04-21
US20230348958A1 (en) 2023-11-02
CA3161593A1 (en) 2021-07-08
JP2023509010A (ja) 2023-03-06
EP4085150A1 (en) 2022-11-09
AU2020418497A1 (en) 2022-04-28
EP4085150A4 (en) 2024-04-17
WO2021138078A1 (en) 2021-07-08

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