JP7849034B2 - クロマチンの３ｄ構成および転写活性のゲノムスケールイメージング - Google Patents

クロマチンの３ｄ構成および転写活性のゲノムスケールイメージング

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Publication number
JP7849034B2
JP7849034B2 JP2022539655A JP2022539655A JP7849034B2 JP 7849034 B2 JP7849034 B2 JP 7849034B2 JP 2022539655 A JP2022539655 A JP 2022539655A JP 2022539655 A JP2022539655 A JP 2022539655A JP 7849034 B2 JP7849034 B2 JP 7849034B2
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nucleic acid
imaging
readout
chromatin
loci
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Japanese (ja)
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JP2023509010A5 (https=
JP2023509010A (ja
Inventor
ジュアン，シャオウェイ
ビントゥ，ボグダン
エス キンロット，ソン
ジェン，プー
ス，ジュンハン
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Harvard University
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Harvard University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional [2D] or three-dimensional [3D] molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/10—Nucleic acid folding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/10—Signal processing, e.g. from mass spectrometry [MS] or from PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional [2D] or three-dimensional [3D] molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/30—Drug targeting using structural data; Docking or binding prediction

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medical Informatics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Theoretical Computer Science (AREA)
  • Zoology (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Computation (AREA)
  • Signal Processing (AREA)
  • Public Health (AREA)
  • Software Systems (AREA)
  • Databases & Information Systems (AREA)
  • Data Mining & Analysis (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Bioethics (AREA)
  • Artificial Intelligence (AREA)
  • Epidemiology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2022539655A 2019-12-30 2020-12-18 クロマチンの３ｄ構成および転写活性のゲノムスケールイメージング Active JP7849034B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962954720P 2019-12-30 2019-12-30
US62/954,720 2019-12-30
US202063060947P 2020-08-04 2020-08-04
US63/060,947 2020-08-04
PCT/US2020/065797 WO2021138078A1 (en) 2019-12-30 2020-12-18 Genome-scale imaging of the 3d organization and transcriptional activity of chromatin

Publications (3)

Publication Number Publication Date
JP2023509010A JP2023509010A (ja) 2023-03-06
JP2023509010A5 JP2023509010A5 (https=) 2024-02-13
JP7849034B2 true JP7849034B2 (ja) 2026-04-21

Family

ID=76687247

Family Applications (1)

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JP2022539655A Active JP7849034B2 (ja) 2019-12-30 2020-12-18 クロマチンの３ｄ構成および転写活性のゲノムスケールイメージング

Country Status (7)

Country Link
US (1) US20230348958A1 (https=)
EP (1) EP4085150A4 (https=)
JP (1) JP7849034B2 (https=)
CN (1) CN115023502A (https=)
AU (1) AU2020418497A1 (https=)
CA (1) CA3161593A1 (https=)
WO (1) WO2021138078A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4328322A3 (en) 2014-07-30 2024-05-22 President and Fellows of Harvard College Probe library construction
EP3894583A4 (en) 2018-12-13 2022-11-09 President and Fellows of Harvard College AMPLIFICATION METHODS AND SYSTEMS FOR MERFISH AND OTHER APPLICATIONS
WO2023097244A1 (en) * 2021-11-24 2023-06-01 Yale University Methods of determining chromatin alterations
WO2023122041A1 (en) * 2021-12-20 2023-06-29 Enumerix, Inc. Detection and digital quantitation of multiple targets
EP4453246A4 (en) 2021-12-20 2025-12-24 Countable Labs Inc DIGITAL DETECTION AND QUANTIFICATION OF MULTIPLE TARGETS
US20240158836A1 (en) * 2022-06-30 2024-05-16 Resolve Biosciences Gmbh High resolution multiplex method for detecting at least two targets with a distance of beyond the diffraction limit in a sample
CN116052759B (zh) * 2022-12-09 2024-07-16 本源量子计算科技（合肥）股份有限公司 一种哈密顿量构造方法及相关装置
CN120721467B (zh) * 2025-08-29 2026-04-03 华中科技大学同济医学院附属协和医院 一种用于定位检测新生rna合成水平的多重免疫荧光染色方法及试剂盒

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170220733A1 (en) 2014-07-30 2017-08-03 President And Fellows Of Harvard College Systems and methods for determining nucleic acids

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016081740A1 (en) * 2014-11-21 2016-05-26 Nanostring Technologies, Inc. Enzyme- and amplification-free sequencing
EP3539036A4 (en) * 2016-11-08 2020-06-17 President and Fellows of Harvard College MATRIX AND DELETE
EP3894583A4 (en) * 2018-12-13 2022-11-09 President and Fellows of Harvard College AMPLIFICATION METHODS AND SYSTEMS FOR MERFISH AND OTHER APPLICATIONS

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170220733A1 (en) 2014-07-30 2017-08-03 President And Fellows Of Harvard College Systems and methods for determining nucleic acids

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Andres M. Cardozo Gizzi et al.，Microscopy-based Cromosome Conformation Capture Enables Simultaneous Visualization of Genome Organization and Transcription in Intact Organisms.，Molecullar Cell，2019年02月，74，212-222
Bogdan Bintu et al.，Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells.，Science，2018年，362，eaau1783，DOI: 10.1126/science.aau1783
Kok Hao Chen et al.，RNA imaging. Spatially resolved, highly multiplexed RNA profiling in single cells.，Science，2015年，348，aaa6090
Siyuan Wang et al.，Spatial organization of chromatin domains and compartments in single chromosomes.，Science，2016年，353，598-602

Also Published As

Publication number Publication date
US20230348958A1 (en) 2023-11-02
CA3161593A1 (en) 2021-07-08
JP2023509010A (ja) 2023-03-06
EP4085150A1 (en) 2022-11-09
AU2020418497A1 (en) 2022-04-28
EP4085150A4 (en) 2024-04-17
CN115023502A (zh) 2022-09-06
WO2021138078A1 (en) 2021-07-08

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