CN115011557B - Non-blood-source nucleated cell experimental identification device and using method thereof - Google Patents
Non-blood-source nucleated cell experimental identification device and using method thereof Download PDFInfo
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- CN115011557B CN115011557B CN202210842355.XA CN202210842355A CN115011557B CN 115011557 B CN115011557 B CN 115011557B CN 202210842355 A CN202210842355 A CN 202210842355A CN 115011557 B CN115011557 B CN 115011557B
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- 238000000034 method Methods 0.000 title description 10
- 230000005540 biological transmission Effects 0.000 claims abstract description 67
- 239000000463 material Substances 0.000 claims abstract description 66
- 238000000926 separation method Methods 0.000 claims abstract description 29
- 238000001914 filtration Methods 0.000 claims abstract description 11
- 239000012528 membrane Substances 0.000 claims abstract description 7
- 238000007599 discharging Methods 0.000 claims description 9
- 238000000429 assembly Methods 0.000 claims description 6
- 230000000712 assembly Effects 0.000 claims description 6
- 239000011521 glass Substances 0.000 abstract description 18
- 239000007788 liquid Substances 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 27
- 210000004369 blood Anatomy 0.000 description 14
- 239000008280 blood Substances 0.000 description 14
- 210000005259 peripheral blood Anatomy 0.000 description 3
- 239000011886 peripheral blood Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 210000003924 normoblast Anatomy 0.000 description 2
- 201000003793 Myelodysplastic syndrome Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 210000003651 basophil Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000003979 eosinophil Anatomy 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 208000007475 hemolytic anemia Diseases 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 210000000948 non-nucleated cell Anatomy 0.000 description 1
- 210000004180 plasmocyte Anatomy 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
- C12N2509/10—Mechanical dissociation
Abstract
The invention discloses a non-blood-source nucleated cell experimental identification device, which comprises: the main body unit comprises an operation table, a separation box is arranged on the operation table, and a microscope equipment body is arranged on the operation table; the material conveying unit comprises a plurality of rotating shafts, rollers are symmetrically and fixedly connected to the rotating shafts, material conveying belts are arranged on the rollers, a plurality of C-shaped plates are arranged on the material conveying belts, a second transmission assembly is arranged on one side of the separating box, and a third transmission assembly is symmetrically arranged between the second transmission assembly and the rotating shaft on the right side. According to the invention, the speed-variable motor rotates at a low speed to enable the round rod to rotate, the rotating shaft is driven to rotate, the roller is driven to rotate, the material conveying belt is driven to move, a worker places a glass slide at the C-shaped plate, separated liquid is dripped on the glass slide through the biological filtering membrane, and then the speed-variable motor is started to transport the glass slide out, so that operations such as filtering and tabletting are not required to be manually performed by a user.
Description
Technical Field
The invention relates to the technical field of cell detection, in particular to a non-blood-source nucleated cell experimental identification device and a use method thereof.
Background
Nucleated cells refer to cells having nuclei, including leukocytes, lymphocytes, monocytes, plasma cells, macrophages, etc., wherein the leukocytes are further classified into neutrophils, eosinophils, and basophils, which are all nucleated cells and are distributed in peripheral blood. Mature erythrocytes are non-nucleated cells, nucleated erythrocytes cannot be seen in normal peripheral blood, abnormal conditions are indicated if nucleated erythrocytes are present in peripheral blood, and can be seen in hemolytic anemia, leukemia, myelodysplastic syndrome and the like, and in some biological experiments, workers need to perform experimental identification on non-blood-derived nucleated cells.
In the identification process, the worker is required to centrifugally separate blood, filter the blood after separation, finally make slices, observe and detect the slices, and the processes of cell extraction, filtration, slice making and the like are all operated manually, so that the workload of the worker is increased, and the working efficiency is lower.
Disclosure of Invention
This section is intended to outline some aspects of embodiments of the application and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description of the application and in the title of the application, which may not be used to limit the scope of the application.
The present invention has been made in view of the above-described problems with the conventional authentication work.
Therefore, the invention aims to provide a non-blood-source nucleated cell experimental identification device and a use method thereof, which aim to solve the problems that in the identification process, a worker is required to centrifugally separate blood, filter the blood after separation, finally perform sheet making, observation and detection, and the processes of cell extraction, filtration, sheet making and the like are all manually operated, so that the workload of the worker is increased, and the work efficiency is lower.
In order to solve the technical problems, the invention provides the following technical scheme:
An experimental identification device for non-blood-borne nucleated cells, comprising:
The main body unit comprises an operation table, wherein a plurality of supporting legs are fixedly connected to the operation table, a separation box is arranged on the operation table, and a microscope equipment body is arranged on the operation table;
The separating unit comprises a material placing tray, the material placing tray is arranged in a separating box, a plurality of clamping assemblies are arranged at the bottom of the material placing tray, a power assembly for driving the clamping assemblies is arranged on the material placing tray, a plurality of round holes are formed in the material placing tray, a material storage pipe is arranged in the round holes, a material discharging pipe is arranged at the bottom of the material storage pipe, a biological filtering membrane is arranged in the material discharging pipe, an electromagnetic valve is arranged on the material discharging pipe, a variable speed motor is arranged on the right side wall of the separating box, a worm is fixedly connected with the output end of the variable speed motor, the worm rotates and is inserted on the separating box, and a first transmission assembly is arranged between the worm and the material placing tray;
The material conveying unit comprises a plurality of rotating shafts, a first opening is formed in the bottom of the separation box, a second opening is formed in the operation table, the rotating shafts are symmetrically and rotationally connected to the inner side wall of the second opening, rollers are symmetrically and fixedly connected to the rotating shafts, material conveying belts are arranged on the rollers, a plurality of C-shaped plates are arranged on the material conveying belts, a second transmission assembly is arranged on one side of the separation box, and a third transmission assembly is symmetrically arranged between the second transmission assembly and the right rotating shaft.
As a preferred embodiment of the non-blood-derived nucleated cell experimental identification apparatus according to the present invention, wherein: the separating box is hinged with a cover plate, and a handle is arranged on the cover plate.
As a preferred embodiment of the non-blood-derived nucleated cell experimental identification apparatus according to the present invention, wherein: clamping assembly, including the dead lever, dead lever bottom fixedly connected with connecting block, the symmetry rotates on the connecting block and is connected with the clamping lever, fixedly connected with splint on the clamping lever, it has the slide bar to slip on the connecting block to alternate, fixedly connected with movable plate on the slide bar, it is connected with the interlock pole to rotate between movable plate and the clamping lever, be equipped with the arc breach on the clamping assembly, all be equipped with the elastic cushion in arc breach and the round hole, the elastic cushion is made for the rubber materials.
As a preferred embodiment of the non-blood-derived nucleated cell experimental identification apparatus according to the present invention, wherein: the power assembly comprises a threaded rod, the threaded rod is rotatably inserted on the material placing disc, a moving block is connected to the threaded rod in a threaded mode, a connecting rod is rotatably connected between the moving block and the sliding rod, and a rotary table is fixedly connected to the top end of the threaded rod.
As a preferred embodiment of the non-blood-derived nucleated cell experimental identification apparatus according to the present invention, wherein: the first transmission assembly comprises a transmission gear, an annular toothed plate is fixedly connected to the upper side wall of the material placing disc, and the transmission gear is matched with the annular toothed plate.
As a preferred embodiment of the non-blood-derived nucleated cell experimental identification apparatus according to the present invention, wherein: the annular toothed plate is rotationally connected with an annular plate on the side wall of the annular toothed plate, and a fixed plate is fixedly connected between the annular plate and the inner side wall of the separation box.
As a preferred embodiment of the non-blood-derived nucleated cell experimental identification apparatus according to the present invention, wherein: the second transmission assembly comprises two mounting plates, the mounting plates are symmetrically and fixedly connected to the separation box, a round rod is rotatably connected between the two mounting plates, a worm wheel is fixedly connected to the round rod, and the worm wheel is meshed with the worm.
As a preferred embodiment of the non-blood-derived nucleated cell experimental identification apparatus according to the present invention, wherein: the third transmission assembly comprises a driving transmission wheel which is fixedly connected to the round rod, a driven transmission wheel is fixedly connected to the rotating shaft, and a transmission belt is arranged between the driving transmission wheel and the driven transmission wheel bracket.
The using method of the non-blood-source nucleated cell experimental identification device comprises the following steps:
When the movable plate is used, the storage tube containing cells to be detected is inserted into the round hole, then the rotary table is rotated to enable the threaded rod to rotate, the movable block is driven to move upwards, the sliding rod is driven to move inwards, the movable plate is driven to move inwards, the linkage rod is driven to rotate, the clamping rod is driven to move inwards, the clamping plate is driven to move inwards, and the storage tube is clamped;
The upper cover plate is covered, the variable speed motor is started to enable the worm to rotate at a high speed, the transmission gear is driven to rotate, the annular toothed plate is driven to rotate, the material placing disc is driven to rotate, and centrifugal separation is carried out on cells in the material storage pipe;
After separation is completed, the variable speed motor rotates at a low speed, the worm rotates to drive the worm wheel to rotate, the round rod rotates to drive the driving transmission wheel to rotate, the driven transmission wheel rotates through the action of the transmission belt to drive the rotating shaft to rotate, the idler wheel rotates to drive the material conveying belt to move, a worker places a glass slide at the C-shaped plate, when the glass slide moves to a certain position, the variable speed motor stops rotating, the material storage pipe is positioned above the glass slide, the electromagnetic valve is opened, the separated liquid is dripped on the glass slide through the biological filtering membrane, and then the variable speed motor is started to convey the glass slide;
The worker takes the slide and places it on the microscope device body to identify it.
The invention has the beneficial effects that:
1. During the use, will hold the storage tube that waits to detect the cell and insert in the round hole, rotate the carousel afterwards for the threaded rod rotates, drives the movable block upward movement, make the sliding rod inward movement, drives the inward movement of movable plate, drives the trace and rotates, make the clamping lever inwards move, drive splint inward movement, carry out the centre gripping to the storage tube, cover the apron later, start gear motor and make worm high-speed rotation, drive gear and rotate, make annular pinion rack rotate, drive and put the charging tray and rotate, carry out centrifugal separation to the cell in the storage tube, easy operation is convenient, work efficiency has been improved.
2. After the separation is completed, the variable speed motor rotates at a low speed, the worm rotates to drive the worm wheel to rotate, the round rod rotates to drive the driving transmission wheel to rotate, the driven transmission wheel rotates through the action of the transmission belt to drive the rotating shaft to rotate, the idler wheel rotates to drive the material conveying belt to move, a worker places the glass slide at the C-shaped plate, when the glass slide moves to a certain position, the variable speed motor stops rotating, the material storage pipe is located above the glass slide, the electromagnetic valve is opened, separated liquid is enabled to drop on the glass slide through the biological filtering membrane, then the variable speed motor is started to transport the glass slide, operations such as filtering sheet making are not needed by a user manually, the workload of the worker is reduced, and the work efficiency is improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:
Fig. 1 is a schematic diagram of the whole structure of a non-blood-derived nucleated cell experimental identification apparatus according to the present invention.
Fig. 2 is a schematic cross-sectional structure of fig. 1.
Fig. 3 is a top view of a partial structure in the separator tank of fig. 2.
Fig. 4 is a schematic view of the bottom structure of the tray in fig. 3.
Fig. 5 is a bottom view of fig. 3.
Fig. 6 is a right side view of fig. 1.
In the figure: 100. a main body unit; 101. an operation table; 102. support legs; 103. a separation box; 104. a cover plate; 105. a handle; 106. a microscope device body; 200. a separation unit; 201. a material placing disc; 202. a clamping assembly; 202a, a fixed rod; 202b, connecting blocks; 202c, clamping rods; 202d, clamping plates; 202e, sliding rod; 202f, a moving plate; 202g, a linkage rod; 202h, an elastic pad; 203. a power assembly; 203a, a threaded rod; 203b, a moving block; 203c, connecting rods; 203d, a turntable; 204. a storage tube; 205. a discharge pipe; 206. an electromagnetic valve; 207. a variable speed motor; 208. a worm; 209. a first transmission assembly; 209a, a transmission gear; 209b, annular toothed plate; 210. an annular plate; 211. a fixing plate; 300. a material conveying unit; 301. a first opening; 302. a second opening; 303. a rotating shaft; 304. a roller; 305. a material conveying belt; 306. a C-shaped plate; 307. a second transmission assembly; 307a, mounting plate; 307b, round bar; 307c, worm gear; 308. a third transmission assembly; 308a, an active driving wheel; 308b, a driven transmission wheel; 308c, a driving belt.
Detailed Description
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present invention is not limited to the specific embodiments disclosed below.
Further, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic can be included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Further, in describing the embodiments of the present invention in detail, the cross-sectional view of the device structure is not partially enlarged to a general scale for convenience of description, and the schematic is only an example, which should not limit the scope of protection of the present invention. In addition, the three-dimensional dimensions of length, width and depth should be included in actual fabrication.
Referring to FIGS. 1-6, the present invention provides an experimental identification apparatus for non-blood-derived nucleated cells, comprising:
the main body unit 100 comprises an operation table 101, a plurality of supporting legs 102 are fixedly connected to the operation table 101, a separation box 103 is arranged on the operation table 101, and a microscope device body 106 is arranged on the operation table 101;
The separating unit 200 comprises a material placing tray 201, wherein the material placing tray 201 is arranged in a separating box 103, a plurality of clamping assemblies 202 are arranged at the bottom of the material placing tray 201, a power assembly 203 for driving the clamping assemblies 202 is arranged on the material placing tray 201, a plurality of round holes are formed in the material placing tray 201, a material storage pipe 204 is arranged in the round holes, a material discharging pipe 205 is arranged at the bottom of the material storage pipe 204, a biological filtering membrane is arranged in the material discharging pipe 205, an electromagnetic valve 206 is arranged on the material discharging pipe 205, a variable speed motor 207 is arranged on the right side wall of the separating box 103, a worm 208 is fixedly connected with the output end of the variable speed motor 207, the worm 208 is rotatably inserted on the separating box 103, and a first transmission assembly 209 is arranged between the worm 208 and the material placing tray 201;
The material conveying unit 300 comprises a plurality of rotating shafts 303, a first opening 301 is formed in the bottom of the separation box 103, a second opening 302 is formed in the operating platform 101, the rotating shafts 303 are symmetrically and rotatably connected to the inner side wall of the second opening 302, idler wheels 304 are symmetrically and fixedly connected to the rotating shafts 303, a material conveying belt 305 is arranged on the idler wheels 304, a plurality of C-shaped plates 306 are arranged on the material conveying belt 305, a second transmission assembly 307 is arranged on one side of the separation box 103, and a third transmission assembly 308 is symmetrically arranged between the second transmission assembly 307 and the right rotating shaft 303.
Wherein, the separation box 103 is hinged with a cover plate 104, and the cover plate 104 is provided with a handle 105, so that a user can conveniently open and close the separation box 103 through the cover plate 104.
Further, the clamping assembly 202 comprises a fixed rod 202a, the bottom end of the fixed rod 202a is fixedly connected with a connecting block 202b, the connecting block 202b is symmetrically and rotationally connected with a clamping rod 202c, the clamping rod 202c is fixedly connected with a clamping plate 202d, a sliding rod 202e is slidably inserted in the connecting block 202b, a moving plate 202f is fixedly connected to the sliding rod 202e, a linkage rod 202g is rotationally connected between the moving plate 202f and the clamping rod 202c, an arc notch is formed in the clamping assembly 202, elastic pads 202h are respectively arranged in the arc notch and the round hole, the elastic pads 202h are made of rubber materials, when the sliding rod 202e moves inwards, the moving plate 202f can be driven to move inwards, the linkage rod 202g rotates, the clamping plate 202d is driven to move inwards, and the storage tube 204 is clamped, and the operation is simple and convenient.
Further, the power assembly 203 includes a threaded rod 203a, the threaded rod 203a is rotatably inserted on the tray 201, a moving block 203b is threadedly connected to the threaded rod 203a, a connecting rod 203c is rotatably connected between the moving block 203b and the sliding rod 202e, and a turntable 203d is fixedly connected to the top end of the threaded rod 203a, so that when the threaded rod 203a rotates, the moving block 203b can be driven to move up and down, so that the connecting rod 203c rotates, and the sliding rod 202e is driven to move.
Further, the first transmission assembly 209 includes a transmission gear 209a, and an annular toothed plate 209b is fixedly connected to an upper sidewall of the tray 201, and the transmission gear 209a is matched with the annular toothed plate 209b, so that when the worm 208 rotates, the transmission gear 209a can be driven to rotate, so that the annular toothed plate 209b rotates to drive the tray 201 to rotate.
Further, an annular plate 210 is rotatably connected to the side wall of the annular toothed plate 209b, and a fixing plate 211 is fixedly connected between the annular plate 210 and the inner side wall of the separation box 103, so that the tray 201 is installed in the separation box 103 through the annular plate 210 and the fixing plate 211.
Further, the second transmission assembly 307 includes two mounting plates 307a, the mounting plates 307a are symmetrically and fixedly connected to the separation box 103, a round rod 307b is rotatably connected between the two mounting plates 307a, a worm wheel 307c is fixedly connected to the round rod 307b, and the worm wheel 307c is engaged with the worm 208, so that when the worm 208 rotates, the worm wheel 307c can be driven to rotate, and the round rod 307b rotates.
Further, the third transmission assembly 308 includes a driving transmission wheel 308a, the driving transmission wheel 308a is fixedly connected to the round bar 307b, the driven transmission wheel 308b is fixedly connected to the rotating shaft 303, and a transmission belt 308c is disposed between the driving transmission wheel 308a and the support of the driven transmission wheel 308b, so that when the round bar 307b rotates, the driving transmission wheel 308a can be driven to rotate, and the driven transmission wheel 308b is driven to rotate under the action of the transmission belt 308c, so that the rotating shaft 303 rotates.
In the use process, the storage tube 204 containing the cells to be detected is inserted into the round hole, then the rotary table 203d is rotated to enable the threaded rod 203a to rotate, the moving block 203b is driven to move upwards, the sliding rod 202e is driven to move inwards, the moving plate 202f is driven to move inwards, the linkage rod 202g is driven to rotate, the clamping rod 202c is driven to move inwards, the clamping plate 202d is driven to move inwards, and the storage tube 204 is clamped; the upper cover plate 104 is covered, the variable speed motor 207 is started to enable the worm 208 to rotate at a high speed, the transmission gear 209a is driven to rotate, the annular toothed plate 209b is driven to rotate, the material placing disc 201 is driven to rotate, and centrifugal separation is carried out on cells in the material storage tube 204; after separation is completed, the variable speed motor 207 rotates at a low speed, the worm 208 rotates to drive the worm wheel 307C to rotate, the round rod 307b rotates to drive the driving transmission wheel 308a to rotate, the driven transmission wheel 308b rotates under the action of the transmission belt 308C to drive the rotating shaft 303 to rotate, the roller 304 rotates to drive the material conveying belt 305 to move, a worker places a glass slide at the C-shaped plate 306, when the glass slide moves to a certain position, the variable speed motor 207 stops rotating, the material storage pipe 204 is positioned above the glass slide at the moment, the electromagnetic valve 206 is opened, separated liquid is dripped on the glass slide through the biological filtering membrane, and then the variable speed motor 207 is started to convey the glass slide; the worker removes the slide and places it on the microscope device body 106 to identify it.
It should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted without departing from the spirit and scope of the technical solution of the present invention, which is intended to be covered in the scope of the claims of the present invention.
Claims (3)
1. The non-blood-source nucleated cell experimental identification device is characterized in that: comprising the following steps:
The main body unit (100) comprises an operation table (101), wherein a plurality of supporting legs (102) are fixedly connected to the operation table (101), a separation box (103) is arranged on the operation table (101), and a microscope equipment body (106) is arranged on the operation table (101);
The separating unit (200) comprises a material placing tray (201), the material placing tray (201) is arranged in a separating box (103), a plurality of clamping assemblies (202) are arranged at the bottom of the material placing tray (201), a power assembly (203) for driving the clamping assemblies (202) is arranged on the material placing tray (201), a plurality of round holes are formed in the material placing tray (201), a material storage pipe (204) is arranged in the round holes, a material discharging pipe (205) is arranged at the bottom of the material storage pipe (204), a biological filtering membrane is arranged in the material discharging pipe (205), an electromagnetic valve (206) is arranged on the material discharging pipe (205), a variable speed motor (207) is arranged on the right side wall of the separating box (103), a worm (208) is fixedly connected to the output end of the variable speed motor (207), the worm (208) is rotatably inserted in the separating box (103), and a first transmission assembly (209) is arranged between the worm (208) and the material placing tray (201).
The material conveying unit (300) comprises a plurality of rotating shafts (303), a first opening (301) is formed in the bottom of the separation box (103), a second opening (302) is formed in the operation table (101), the rotating shafts (303) are symmetrically and rotationally connected to the inner side wall of the second opening (302), rollers (304) are symmetrically and fixedly connected to the rotating shafts (303), material conveying belts (305) are arranged on the rollers (304), a plurality of C-shaped plates (306) are arranged on the material conveying belts (305), a second transmission assembly (307) is arranged on one side of the separation box (103), and a third transmission assembly (308) is symmetrically arranged between the second transmission assembly (307) and the right rotating shaft (303);
The clamping assembly (202) comprises a fixed rod (202 a), a connecting block (202 b) is fixedly connected to the bottom end of the fixed rod (202 a), a clamping rod (202 c) is symmetrically and rotationally connected to the connecting block (202 b), a clamping plate (202 d) is fixedly connected to the clamping rod (202 c), a sliding rod (202 e) is inserted in the connecting block (202 b) in a sliding mode, a moving plate (202 f) is fixedly connected to the sliding rod (202 e), a linkage rod (202 g) is rotationally connected between the moving plate (202 f) and the clamping rod (202 c), an arc-shaped notch is formed in the clamping assembly (202), elastic pads (202 h) are arranged in the arc-shaped notch and the round hole, and the elastic pads (202 h) are made of rubber materials;
the power assembly (203) comprises a threaded rod (203 a), the threaded rod (203 a) is rotatably inserted on the material placing disc (201), a moving block (203 b) is connected to the threaded rod (203 a) in a threaded mode, a connecting rod (203 c) is rotatably connected between the moving block (203 b) and the sliding rod (202 e), and a rotary disc (203 d) is fixedly connected to the top end of the threaded rod (203 a);
The first transmission assembly (209) comprises a transmission gear (209 a), an annular toothed plate (209 b) is fixedly connected to the upper side wall of the material placing disc (201), and the transmission gear (209 a) is matched with the annular toothed plate (209 b);
The second transmission assembly (307) comprises two mounting plates (307 a), the mounting plates (307 a) are symmetrically and fixedly connected to the separation box (103), a round rod (307 b) is rotatably connected between the two mounting plates (307 a), a worm wheel (307 c) is fixedly connected to the round rod (307 b), and the worm wheel (307 c) is in meshed connection with the worm (208);
The third transmission assembly (308) comprises a driving transmission wheel (308 a), the driving transmission wheel (308 a) is fixedly connected to the round rod (307 b), the rotating shaft (303) is fixedly connected with a driven transmission wheel (308 b), and a transmission belt (308 c) is arranged between the driving transmission wheel (308 a) and a driven transmission wheel (308 b) support.
2. The non-blood-borne nucleated cell experimental identification device according to claim 1, wherein: the separating box (103) is hinged with a cover plate (104), and a handle (105) is arranged on the cover plate (104).
3. The non-blood-borne nucleated cell experimental identification device according to claim 2, wherein: the annular toothed plate (209 b) is rotationally connected with an annular plate (210), and a fixed plate (211) is fixedly connected between the annular plate (210) and the inner side wall of the separation box (103).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210842355.XA CN115011557B (en) | 2022-07-18 | 2022-07-18 | Non-blood-source nucleated cell experimental identification device and using method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210842355.XA CN115011557B (en) | 2022-07-18 | 2022-07-18 | Non-blood-source nucleated cell experimental identification device and using method thereof |
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| CN115011557A CN115011557A (en) | 2022-09-06 |
| CN115011557B true CN115011557B (en) | 2024-04-26 |
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