CN115011557A - Experimental identification device for non-blood-borne nucleated cells and application method thereof - Google Patents
Experimental identification device for non-blood-borne nucleated cells and application method thereof Download PDFInfo
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- CN115011557A CN115011557A CN202210842355.XA CN202210842355A CN115011557A CN 115011557 A CN115011557 A CN 115011557A CN 202210842355 A CN202210842355 A CN 202210842355A CN 115011557 A CN115011557 A CN 115011557A
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- 238000000034 method Methods 0.000 title claims description 13
- 239000000463 material Substances 0.000 claims abstract description 66
- 230000005540 biological transmission Effects 0.000 claims abstract description 64
- 238000000926 separation method Methods 0.000 claims abstract description 37
- 239000011521 glass Substances 0.000 claims abstract description 18
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 239000012528 membrane Substances 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 210000004369 blood Anatomy 0.000 claims description 24
- 239000008280 blood Substances 0.000 claims description 24
- 208000013210 hematogenous Diseases 0.000 claims description 4
- 238000002474 experimental method Methods 0.000 claims 1
- 230000000712 assembly Effects 0.000 abstract description 5
- 238000000429 assembly Methods 0.000 abstract description 5
- 210000004027 cell Anatomy 0.000 description 28
- 238000007599 discharging Methods 0.000 description 6
- 230000033001 locomotion Effects 0.000 description 6
- 210000005259 peripheral blood Anatomy 0.000 description 3
- 239000011886 peripheral blood Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 210000003924 normoblast Anatomy 0.000 description 2
- 201000003793 Myelodysplastic syndrome Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 210000003651 basophil Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003979 eosinophil Anatomy 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 208000007475 hemolytic anemia Diseases 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 210000004180 plasmocyte Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
- C12N2509/10—Mechanical dissociation
Abstract
The invention discloses a non-blood-borne nucleated cell experimental identification device, which comprises: the main body unit comprises an operation table, wherein a separation box is arranged on the operation table, and a microscope equipment body is arranged on the operation table; the material conveying unit comprises a plurality of rotating shafts, wherein the rotating shafts are symmetrically and fixedly connected with idler wheels, material conveying belts are arranged on the idler wheels, a plurality of C-shaped plates are arranged on the material conveying belts, a second transmission assembly is arranged on one side of the separation box, and third transmission assemblies are symmetrically arranged between the second transmission assembly and the right rotating shaft. According to the invention, the round rod is rotated through the low-speed rotation of the variable-speed motor, the rotating shaft is driven to rotate, the roller is driven to rotate, the conveying belt is driven to move, a worker places a glass slide at the position of the C-shaped plate, separated liquid is dripped on the glass slide through the biological filtering membrane, and then the variable-speed motor is started to convey the glass slide out, so that the manual operation of filtering, sheet making and the like by a user is not needed.
Description
Technical Field
The invention relates to the technical field of cell detection, in particular to a non-blood-derived nucleated cell experimental identification device and a using method thereof.
Background
The nucleated cells refer to cells having nuclei, including leukocytes, lymphocytes, monocytes, plasma cells, macrophages, and the like, wherein the leukocytes are classified into neutrophils, eosinophils, and basophils, which belong to the nucleated cells and are distributed in peripheral blood. Mature erythrocytes are anucleated cells, nucleated erythrocytes cannot be seen in normal peripheral blood, and if nucleated erythrocytes appear in peripheral blood, abnormality is suggested, which can be seen in hemolytic anemia, leukemia, myelodysplastic syndrome and the like.
The identification process needs the staff to firstly carry out centrifugal separation on blood, filters the blood after separation, finally carries out flaking, observes and detects, and processes such as extraction, filtration, flaking of cell are manual operation, have increased staff's work load, and work efficiency is lower.
Disclosure of Invention
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and title of the application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.
The present invention has been made in view of the above-mentioned problems with the prior authentication work.
Therefore, the invention aims to provide a non-blood-derived nucleated cell experimental identification device and a using method thereof, which aim to solve the problems that in the identification process, workers need to firstly carry out centrifugal separation on blood, filter the blood after separation, finally slice the blood, observe and detect the blood, and the processes of cell extraction, filtration, slice making and the like are all manual operation, so that the workload of the workers is increased, and the working efficiency is low.
In order to solve the technical problems, the invention provides the following technical scheme:
an experimental identification device for non-blood-derived nucleated cells, comprising:
the microscope equipment comprises a main body unit and a control unit, wherein the main body unit comprises an operation table, a plurality of supporting legs are fixedly connected to the operation table, a separation box is arranged on the operation table, and a microscope equipment body is arranged on the operation table;
the separation unit comprises a material placing disc, the material placing disc is arranged in the separation box, a plurality of clamping assemblies are arranged at the bottom of the material placing disc, a power assembly used for driving the clamping assemblies is arranged on the material placing disc, a plurality of round holes are formed in the material placing disc, material storage pipes are arranged in the round holes, a material discharging pipe is arranged at the bottom of the material storage pipes, a biological filtering membrane is arranged in the material discharging pipe, an electromagnetic valve is arranged on the material discharging pipe, a variable speed motor is arranged on the right side wall of the separation box, a worm is fixedly connected to the output end of the variable speed motor, the worm is rotatably inserted in the separation box, and a first transmission assembly is arranged between the worm and the material placing disc;
defeated material unit, including a plurality of pivots, the separator box bottom has seted up first opening, the second opening has been seted up on the operation panel, and is a plurality of pivot symmetry is rotated and is connected on the second opening inside wall, symmetry fixedly connected with gyro wheel in the pivot, be equipped with defeated material area on the gyro wheel, be equipped with a plurality of C-shaped plates on the defeated material area, separator box one side is equipped with second transmission assembly, the symmetry is equipped with third transmission assembly between second transmission assembly and the right side pivot.
As a preferable embodiment of the experimental identification device for non-blood-derived nucleated cells of the present invention, wherein: the separating box is hinged with a cover plate, and a handle is arranged on the cover plate.
As a preferable embodiment of the experimental identification device for non-blood-derived nucleated cells of the present invention, wherein: the clamping assembly comprises a fixing rod, a connecting block is fixedly connected to the bottom end of the fixing rod, clamping rods are connected to the connecting block in a symmetrical rotating mode, clamping plates are fixedly connected to the clamping rods, sliding rods are inserted into the connecting block in a sliding mode, a moving plate is fixedly connected to the sliding rods, linkage rods are connected between the moving plate and the clamping rods in a rotating mode, arc-shaped notches are formed in the clamping assembly, elastic pads are arranged in the arc-shaped notches and the round holes, and the elastic pads are made of rubber materials.
As a preferable embodiment of the experimental identification device for non-blood-derived nucleated cells of the present invention, wherein: the power assembly comprises a threaded rod, the threaded rod is inserted into the material placing disc in a rotating mode, a moving block is connected to the threaded rod in a threaded mode, a connecting rod is connected between the moving block and the sliding rod in a rotating mode, and a rotary disc is fixedly connected to the top end of the threaded rod.
As a preferable embodiment of the experimental identification device for non-blood-derived nucleated cells of the present invention, wherein: the first transmission assembly comprises a transmission gear, an annular toothed plate is fixedly connected to the upper side wall of the material placing plate, and the transmission gear is matched with the annular toothed plate.
As a preferable embodiment of the experimental identification device for non-blood-derived nucleated cells of the present invention, wherein: the annular toothed plate side wall is connected with an annular plate in a rotating mode, and a fixing plate is fixedly connected between the annular plate and the inner side wall of the separation box.
As a preferable embodiment of the experimental identification device for non-blood-derived nucleated cells of the present invention, wherein: the second transmission assembly comprises two mounting plates, the mounting plates are symmetrically fixedly connected to the separating box, two round rods are rotatably connected between the mounting plates, worm wheels are fixedly connected to the round rods, and the worm wheels are meshed with the worms.
As a preferable embodiment of the experimental identification device for non-blood-derived nucleated cells of the present invention, wherein: the third transmission assembly comprises a driving transmission wheel, the driving transmission wheel is fixedly connected to the round rod, a driven transmission wheel is fixedly connected to the rotating shaft, and a transmission belt is arranged between the driving transmission wheel and the driven transmission wheel support.
The application method of the experimental identification device for the non-blood-derived nucleated cells comprises the following steps:
when the cell clamping device is used, the storage tube containing cells to be detected is inserted into the round hole, then the rotary table is rotated, the threaded rod is rotated, the movable block is driven to move upwards, the sliding rod is driven to move inwards, the movable plate is driven to move inwards, the linkage rod is driven to rotate, the clamping rod is driven to move inwards, the clamping plate is driven to move inwards, and the storage tube is clamped;
covering the upper cover plate, starting the variable speed motor to enable the worm to rotate at a high speed, driving the transmission gear to rotate, enabling the annular toothed plate to rotate, driving the material placing disc to rotate, and performing centrifugal separation on cells in the material storing pipe;
after separation is completed, the variable speed motor rotates at a low speed, the worm rotates to drive the worm wheel to rotate, so that the round rod rotates to drive the driving transmission wheel to rotate, the driven transmission wheel rotates under the action of the transmission belt to drive the rotating shaft to rotate, the roller rotates to drive the conveying belt to move, a worker places a glass slide on the C-shaped plate, when the glass slide moves to a certain position, the variable speed motor stops rotating, the storage pipe is positioned above the glass slide at the moment, the electromagnetic valve is opened to enable separated liquid to drip on the glass slide through the biofiltration membrane, and then the variable speed motor is started to convey the glass slide out;
the worker takes the slide off and places it on the microscope apparatus body to identify it.
The invention has the beneficial effects that:
1. during the use, insert the storage pipe that waits to detect the cell into the round hole, rotate the carousel afterwards, make the threaded rod rotate, drive the movable block upward movement, make the slide bar inward movement, drive the movable plate inward movement, it rotates to drive the trace, make the supporting rod inward movement, drive splint inward movement, carry out the centre gripping to the storage pipe, cover the apron afterwards, start variable speed motor and make the worm high-speed rotatory, drive gear rotates, make the annular toothed plate rotate, the drive is put the charging tray and is rotated, carry out centrifugal separation to the cell in the storage pipe, and easy operation is convenient, and the work efficiency is improved.
2. After the separation is accomplished, the variable speed motor low-speed rotates, the rotation of worm drives the worm wheel and rotates, make the round bar rotate, it rotates to drive the initiative drive wheel, make driven drive wheel rotate through the effect of drive belt, it rotates to drive the pivot, make the gyro wheel rotate, drive the conveying belt motion, the slide glass is placed in C shaped plate department to the staff, when the slide glass moves to a certain position, variable speed motor stall, the storage tube is located the slide glass top this moment, open the solenoid valve, make the liquid after the separation drip on the slide glass through biofiltration membrane, start variable speed motor afterwards and transport out the slide glass, need not the manual operation such as filtration film-making of user, the work load of staff has been reduced, and the work efficiency is improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise. Wherein:
fig. 1 is a schematic front overall structure diagram of a non-hematogenous nucleated cell experimental identification device according to the present invention.
Fig. 2 is a schematic cross-sectional view of fig. 1.
Fig. 3 is a top view of a portion of the separator tank of fig. 2.
Fig. 4 is a schematic view of the bottom structure of the material tray in fig. 3.
Fig. 5 is a bottom view of fig. 3.
Fig. 6 is a right side view of fig. 1.
In the figure: 100. a main body unit; 101. an operation table; 102. supporting legs; 103. a separation tank; 104. a cover plate; 105. a handle; 106. a microscope apparatus body; 200. a separation unit; 201. placing a material tray; 202. a clamping assembly; 202a, a fixing rod; 202b, a connecting block; 202c, a clamping rod; 202d, clamping plate; 202e, a slide bar; 202f, moving plate; 202g, a linkage rod; 202h, an elastic pad; 203. a power assembly; 203a, a threaded rod; 203b, a moving block; 203c, a connecting rod; 203d, a turntable; 204. a storage pipe; 205. a discharge pipe; 206. an electromagnetic valve; 207. a variable speed motor; 208. a worm; 209. a first transmission assembly; 209a, a transmission gear; 209b, annular toothed plate; 210. an annular plate; 211. a fixing plate; 300. a material conveying unit; 301. a first opening; 302. a second opening; 303. a rotating shaft; 304. a roller; 305. a material conveying belt; 306. a C-shaped plate; 307. a second transmission assembly; 307a, a mounting plate; 307b, a round bar; 307c, a worm gear; 308. a third transmission assembly; 308a, a driving transmission wheel; 308b, a driven transmission wheel; 308c, a belt.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Furthermore, the references herein to "one embodiment" or "an embodiment" refer to a particular feature, structure, or characteristic that may be included in at least one implementation of the present invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Furthermore, the present invention is described in detail with reference to the drawings, and for convenience of illustration, the cross-sectional views illustrating the device structures are not enlarged partially according to the general scale when describing the embodiments of the present invention, and the drawings are only exemplary, which should not limit the scope of the present invention. In addition, the three-dimensional dimensions of length, width and depth should be included in the actual fabrication.
Referring to fig. 1 to 6, the present invention provides an experimental identification apparatus for non-blood-derived nucleated cells, comprising:
the microscope equipment comprises a main body unit 100 and a microscope equipment body unit, wherein the main body unit 100 comprises an operating platform 101, a plurality of supporting legs 102 are fixedly connected to the operating platform 101, a separation box 103 is arranged on the operating platform 101, and the microscope equipment body 106 is arranged on the operating platform 101;
the separation unit 200 comprises a material placing disc 201, the material placing disc 201 is arranged in the separation box 103, a plurality of clamping assemblies 202 are arranged at the bottom of the material placing disc 201, a power assembly 203 for driving the clamping assemblies 202 is arranged on the material placing disc 201, a plurality of round holes are formed in the material placing disc 201, a material storage pipe 204 is arranged in each round hole, a material discharging pipe 205 is arranged at the bottom of each material storage pipe 204, a biological filtering membrane is arranged in each material discharging pipe 205, an electromagnetic valve 206 is arranged on each material discharging pipe 205, a variable speed motor 207 is arranged on the right side wall of the separation box 103, the output end of each variable speed motor 207 is fixedly connected with a worm 208, each worm 208 is rotatably inserted into the separation box 103, and a first transmission assembly 209 is arranged between each worm 208 and the material placing disc 201;
defeated material unit 300, including a plurality of pivots 303, first opening 301 has been seted up to separator box 103 bottom, second opening 302 has been seted up on the operation panel 101, a plurality of pivots 303 symmetry rotate to be connected on second opening 302 inside wall, symmetry fixedly connected with gyro wheel 304 on the pivot 303, be equipped with defeated material area 305 on the gyro wheel 304, be equipped with a plurality of C-shaped boards 306 on the defeated material area 305, separator box 103 one side is equipped with second drive assembly 307, the symmetry is equipped with third drive assembly 308 between second drive assembly 307 and the right side pivot 303.
Wherein, the separating box 103 is hinged with a cover plate 104, the cover plate 104 is provided with a handle 105, and a user can open and close the separating box 103 conveniently through the cover plate 104.
Further, the clamping assembly 202 comprises a fixing rod 202a, a connecting block 202b is fixedly connected to the bottom end of the fixing rod 202a, a clamping rod 202c is symmetrically and rotatably connected to the connecting block 202b, a clamping plate 202d is fixedly connected to the clamping rod 202c, a sliding rod 202e is slidably inserted into the connecting block 202b, a moving plate 202f is fixedly connected to the sliding rod 202e, a linkage rod 202g is rotatably connected between the moving plate 202f and the clamping rod 202c, an arc-shaped notch is formed in the clamping assembly 202, elastic pads 202h are arranged in the arc-shaped notch and the round hole, and the elastic pads 202h are made of rubber materials, so that when the sliding rod 202e moves inwards, the moving plate 202f can be driven to move inwards, the linkage rod 202g is rotated, the clamping plate 202d is driven to move inwards, the storage pipe 204 is clamped, and the operation is simple and convenient.
Further, the power assembly 203 comprises a threaded rod 203a, the threaded rod 203a rotates and penetrates through the material placing disc 201, a moving block 203b is connected to the threaded rod 203a in a threaded mode, a connecting rod 203c is connected between the moving block 203b and the sliding rod 202e in a rotating mode, and a rotating disc 203d is fixedly connected to the top end of the threaded rod 203a, so that when the threaded rod 203a rotates, the moving block 203b can be driven to move up and down, the connecting rod 203c rotates, and the sliding rod 202e is driven to move.
Further, the first transmission assembly 209 includes a transmission gear 209a, an annular toothed plate 209b is fixedly connected to the upper side wall of the material placing plate 201, and the transmission gear 209a is matched with the annular toothed plate 209b, so that when the worm 208 rotates, the transmission gear 209a can be driven to rotate, the annular toothed plate 209b can be driven to rotate, and the material placing plate 201 is driven to rotate.
Further, an annular plate 210 is rotatably connected to the side wall of the annular toothed plate 209b, and a fixing plate 211 is fixedly connected between the annular plate 210 and the inner side wall of the separation box 103, so that the material placing disc 201 is installed in the separation box 103 through the annular plate 210 and the fixing plate 211.
Further, the second transmission assembly 307 includes two mounting plates 307a, the mounting plates 307a are symmetrically and fixedly connected to the separation box 103, a circular rod 307b is rotatably connected between the two mounting plates 307a, a worm wheel 307c is fixedly connected to the circular rod 307b, and the worm wheel 307c is engaged with the worm 208, so that when the worm 208 rotates, the worm wheel 307c is driven to rotate, and the circular rod 307b rotates.
Furthermore, the third transmission assembly 308 includes a driving transmission wheel 308a, the driving transmission wheel 308a is fixedly connected to the round bar 307b, the rotating shaft 303 is fixedly connected to the driven transmission wheel 308b, and a transmission belt 308c is disposed between the driving transmission wheel 308a and the bracket of the driven transmission wheel 308b, so that when the round bar 307b rotates, the driving transmission wheel 308a is driven to rotate, and the driven transmission wheel 308b is driven to rotate through the action of the transmission belt 308c, so that the rotating shaft 303 rotates.
In the using process, the storage tube 204 containing the cells to be detected is inserted into the circular hole, then the rotary disc 203d is rotated, so that the threaded rod 203a rotates to drive the moving block 203b to move upwards, the sliding rod 202e moves inwards, the moving plate 202f is driven to move inwards, the linkage rod 202g is driven to rotate, the clamping rod 202c moves inwards, the clamping plate 202d is driven to move inwards, and the storage tube 204 is clamped; the upper cover plate 104 is covered, the variable speed motor 207 is started to enable the worm 208 to rotate at a high speed, the transmission gear 209a is driven to rotate, the annular toothed plate 209b is driven to rotate, the material placing disc 201 is driven to rotate, and centrifugal separation is carried out on cells in the material storing pipe 204; after separation is completed, the variable speed motor 207 rotates at a low speed, the worm 208 rotates to drive the worm wheel 307C to rotate, so that the round rod 307b rotates to drive the driving transmission wheel 308a to rotate, the driven transmission wheel 308b rotates under the action of the transmission belt 308C to drive the rotating shaft 303 to rotate, the roller 304 rotates to drive the material conveying belt 305 to move, a worker places a slide on the C-shaped plate 306, when the slide moves to a certain position, the variable speed motor 207 stops rotating, the material storage pipe 204 is positioned above the slide at the moment, the electromagnetic valve 206 is opened to enable separated liquid to drip on the slide through the biological filter membrane, and then the variable speed motor 207 is started to transport the slide out; the worker takes the slide off and places it on the microscope apparatus body 106 to authenticate it.
It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (9)
1. The utility model provides a non-hematogenous nucleated cell experiment identification device which characterized in that: the method comprises the following steps:
the microscope equipment comprises a main body unit (100) and a control unit, wherein the main body unit comprises an operation table (101), a plurality of supporting legs (102) are fixedly connected to the operation table (101), a separation box (103) is arranged on the operation table (101), and a microscope equipment body (106) is arranged on the operation table (101);
the separation unit (200) comprises a material placing disc (201), the material placing disc (201) is arranged in the separation box (103), the bottom of the material placing plate (201) is provided with a plurality of clamping components (202), the material placing plate (201) is provided with a power component (203) for driving the clamping components (202), a plurality of round holes are arranged on the material placing plate (201), a material storage pipe (204) is arranged in the round holes, a discharge pipe (205) is arranged at the bottom of the storage pipe (204), a biological filtering membrane is arranged in the discharge pipe (205), an electromagnetic valve (206) is arranged on the discharge pipe (205), a variable speed motor (207) is arranged on the right side wall of the separation box (103), the output end of the variable speed motor (207) is fixedly connected with a worm (208), the worm (208) is rotatably inserted on the separation box (103), a first transmission component (209) is arranged between the worm (208) and the material placing disc (201);
defeated material unit (300), including a plurality of pivots (303), first opening (301) have been seted up to separator box (103) bottom, second opening (302) have been seted up on operation panel (101), and are a plurality of pivot (303) symmetry is rotated and is connected on second opening (302) inside wall, symmetry fixedly connected with gyro wheel (304) are gone up in pivot (303), be equipped with on gyro wheel (304) and defeated material area (305), be equipped with a plurality of C shaped plates (306) on defeated material area (305), separator box (103) one side is equipped with second drive assembly (307), the symmetry is equipped with third drive assembly (308) between second drive assembly (307) and right side pivot (303).
2. The experimental identification device for non-blood-derived nucleated cells according to claim 1, wherein: the separating box (103) is hinged with a cover plate (104), and a handle (105) is arranged on the cover plate (104).
3. The experimental identification device for non-blood-derived nucleated cells according to claim 2, wherein: the clamping assembly (202) comprises a fixing rod (202a), a connecting block (202b) is fixedly connected to the bottom end of the fixing rod (202a), a clamping rod (202c) is symmetrically and rotatably connected to the connecting block (202b), a clamping plate (202d) is fixedly connected to the clamping rod (202c), a sliding rod (202e) is slidably inserted into the connecting block (202b), a moving plate (202f) is fixedly connected to the sliding rod (202e), a linkage rod (202g) is rotatably connected between the moving plate (202f) and the clamping rod (202c), an arc-shaped notch is formed in the clamping assembly (202), an elastic pad (202h) is arranged in the arc-shaped notch and the round hole, and the elastic pad (202h) is made of rubber materials.
4. The device for experimental identification of non-blood-derived nucleated cells according to claim 3, wherein: the power assembly (203) comprises a threaded rod (203a), the threaded rod (203a) is rotatably inserted into the material placing disc (201), a moving block (203b) is connected to the threaded rod (203a) in a threaded mode, a connecting rod (203c) is rotatably connected between the moving block (203b) and the sliding rod (202e), and a rotating disc (203d) is fixedly connected to the top end of the threaded rod (203 a).
5. The device for experimental identification of non-blood-derived nucleated cells according to claim 4, wherein: the first transmission assembly (209) comprises a transmission gear (209a), an annular toothed plate (209b) is fixedly connected to the upper side wall of the material placing plate (201), and the transmission gear (209a) is matched with the annular toothed plate (209 b).
6. The device for experimental identification of non-blood-derived nucleated cells according to claim 5, wherein: the annular toothed plate (209b) is connected with an annular plate (210) in a rotating mode on the side wall, and a fixing plate (211) is fixedly connected between the annular plate (210) and the inner side wall of the separation box (103).
7. The device for experimental identification of non-hematogenous nucleated cells according to claim 6, wherein: the second transmission assembly (307) comprises two mounting plates (307a), the mounting plates (307a) are symmetrically and fixedly connected to the separating box (103), a round rod (307b) is rotatably connected between the mounting plates (307a), a worm wheel (307c) is fixedly connected to the round rod (307b), and the worm wheel (307c) is meshed with the worm (208).
8. The experimental identification device of non-hematogenous nucleated cells according to claim 7, wherein: the third transmission assembly (308) comprises a driving transmission wheel (308a), the driving transmission wheel (308a) is fixedly connected to a round rod (307b), a driven transmission wheel (308b) is fixedly connected to the rotating shaft (303), and a transmission belt (308c) is arranged between the driving transmission wheel (308a) and a bracket of the driven transmission wheel (308 b).
9. The use method of the experimental identification device for the non-blood-derived nucleated cells is characterized in that: the method comprises the following steps:
1) when the cell clamping device is used, a storage tube (204) containing cells to be detected is inserted into the round hole, then the rotary disc (203d) is rotated, the threaded rod (203a) is rotated to drive the moving block (203b) to move upwards, the sliding rod (202e) moves inwards, the moving plate (202f) is driven to move inwards, the linkage rod (202g) is driven to rotate, the clamping rod (202c) moves inwards, the clamping plate (202d) is driven to move inwards, and the storage tube (204) is clamped;
2) the upper cover plate (104) is covered, the variable speed motor (207) is started to enable the worm (208) to rotate at a high speed, the transmission gear (209a) is driven to rotate, the annular toothed plate (209b) is driven to rotate, the material placing disc (201) is driven to rotate, and cells in the material storage pipe (204) are subjected to centrifugal separation;
3) after separation is finished, the variable-speed motor (207) rotates at a low speed, the worm (208) rotates to drive the worm wheel (307C) to rotate, the round rod (307b) rotates to drive the driving transmission wheel (308a) to rotate, the driven transmission wheel (308b) rotates through the action of the transmission belt (308C), the rotating shaft (303) is driven to rotate, the roller (304) rotates to drive the material conveying belt (305) to move, a worker places a glass slide on the C-shaped plate (306), when the glass slide moves to a certain position, the variable-speed motor (207) stops rotating, the material storage pipe (204) is positioned above the glass slide at the moment, the electromagnetic valve (206) is opened, so that separated liquid is dripped on the glass slide through the biological filter membrane, and then the variable-speed motor (207) is started to convey the glass slide out;
4) the worker takes the slide and places it on the microscope apparatus body (106) to authenticate it.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210842355.XA CN115011557B (en) | 2022-07-18 | Non-blood-source nucleated cell experimental identification device and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210842355.XA CN115011557B (en) | 2022-07-18 | Non-blood-source nucleated cell experimental identification device and using method thereof |
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| CN115011557A true CN115011557A (en) | 2022-09-06 |
| CN115011557B CN115011557B (en) | 2024-04-26 |
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