CN115011499B - Potato starch wastewater treatment microbial inoculum - Google Patents

Potato starch wastewater treatment microbial inoculum Download PDF

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CN115011499B
CN115011499B CN202210010239.1A CN202210010239A CN115011499B CN 115011499 B CN115011499 B CN 115011499B CN 202210010239 A CN202210010239 A CN 202210010239A CN 115011499 B CN115011499 B CN 115011499B
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potato starch
biochar
strain
starch wastewater
potato
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CN115011499A (en
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陈正军
霍荣帆
何文文
张瀚文
刘垒
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Gansu Agricultural University
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Gansu Agricultural University
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10B—DESTRUCTIVE DISTILLATION OF CARBONACEOUS MATERIALS FOR PRODUCTION OF GAS, COKE, TAR, OR SIMILAR MATERIALS
    • C10B53/00—Destructive distillation, specially adapted for particular solid raw materials or solid raw materials in special form
    • C10B53/02—Destructive distillation, specially adapted for particular solid raw materials or solid raw materials in special form of cellulose-containing material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F2003/001—Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms
    • C02F2003/003—Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms using activated carbon or the like
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/26—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Abstract

The invention provides a potato starch wastewater treatment microbial inoculum, which comprises a bacillus belicus strain and is characterized in that the strain is preserved in the China collection culture Collection, address: in the eight-channel 299-grade university of Wuhan in Wuhan district of Hubei province, the preservation number is CCTCC M20211501. The invention also provides a method for preparing the wastewater treatment microbial inoculum by using the strain, and the strain and the microbial inoculum can effectively degrade potato starch wastewater.

Description

Potato starch wastewater treatment microbial inoculum
Technical Field
The invention relates to the technical field of microorganisms, in particular to a preparation method and application of a potato starch wastewater treatment microbial inoculum.
Background
Along with increasingly prominent ecological civilization construction status and effect in China, the problem of reasonably and economically degrading high-concentration organic wastewater generated in the potato starch processing process and utilizing potato residues becomes very prominent in the potato production industry. Research shows that 1t of potato processing starch can produce 0.7-1.0 t of high-concentration organic wastewater, the wastewater is mainly potato cell sap and starch washing water, and serious environmental pollution can be caused by improper discharge, so that the production and living environments of people are damaged. At present, biological treatment methods are commonly adopted as potato starch wastewater treatment methods by enterprises at home and abroad. The biological treatment method mainly comprises three main types of anaerobic biological treatment method, aerobic biological treatment method and combined biological treatment method. Common biological treatment devices are upflow anaerobic sludge beds, anaerobic expanded granular sludge beds, anaerobic baffled reactors, microbial fuel cells, membrane bioreactors, and the like. The core of the biological treatment devices is to inoculate anaerobic sludge or activated sludge and then form a core functional microorganism group with the potato starch wastewater treatment capability through domestication. However, the growth of microorganisms has high temperature requirements, most microorganisms grow at a suitable temperature of about 25 ℃, and the growth of microorganisms in low temperature environments is widely limited. Therefore, a need exists for a method for efficiently restoring high-concentration potato starch wastewater in a low-temperature environment.
The immobilized microorganism technology is to fix the selected microorganism on a carrier without toxic action, limit the space area of the activity, and has high density and longer biological activity. Can proliferate rapidly and in large quantity under proper conditions, and can maintain the self-activity for a long time under relatively poor conditions. The immobilized microbial agent has the characteristics of easy control of biological concentration, strong poison resistance, low external environment requirement and the like. In recent years, the research of immobilized microorganism technology is very active, and the immobilized microorganism technology is widely applied to various fields such as environmental protection, food industry, medicine and pharmacy. The biochar is used as one of excellent carriers of immobilized microorganisms, and has the characteristics of large specific surface area and rich porosity. The biochar can provide a good growth environment for the growth and propagation of microorganisms, and a stable surrounding environment for the microorganisms attached to the biochar, so that the strain has wider adaptability to temperature and pH value. The degradation effect is improved through the immobilization of the biochar, and meanwhile, the solid microbial inoculum is convenient to store and transport and has important significance for reducing the transportation and use cost of the microbial inoculum.
Therefore, the microbial agent is prepared by adopting the potato starch wastewater treatment bacterial strain separated and screened from the potato starch wastewater sediment, so that the microbial agent can be rapidly proliferated in a natural environment and can be used as a potato starch wastewater treatment bacterial agent for instant use, so as to solve the problem of high-efficiency treatment of potato starch wastewater.
Problems of the prior art:
(1) The potato starch processing production has seasonality, the potato starch processing time in China is mainly concentrated in the middle ten days of August to the next month of Japan, most of the processing time is in winter, and the environment temperature is low. At present, the biological treatment method has higher temperature requirement, and some biological treatment methods even need to control the temperature to 35 ℃, so that a great amount of energy is definitely required to be consumed, and the burden of treating potato starch wastewater of potato starch processing enterprises is increased. Under the low-temperature condition, the existing biological treatment method is low in treatment efficiency, and the death phenomenon of a large amount of core functional strains for degrading potato starch wastewater often occurs, so that the degradation performance of the strains on the potato starch wastewater is seriously affected.
(2) The potato starch wastewater has the characteristics of high COD concentration, intermittent discharge and large single discharge amount. At present, biological treatment devices such as up-flow anaerobic sludge beds, expanded granular sludge beds, anaerobic internal circulation reactors and the like have poor shock resistance. After multiple uses, the biological treatment devices have gradually reduced degradation capacity to potato starch wastewater because of reduced loss of internal strains. Therefore, these biological treatment devices often require replacement of their internal bacterial species to maintain the effectiveness of degradation of potato starch wastewater.
Disclosure of Invention
In view of the defects of the prior art, the application aims to solve the problems that the degradation efficiency of potato starch wastewater in the actual environment is low and the impact resistance of a biological treatment device is poor, and bacterial strains capable of degrading potato starch wastewater are separated and screened from potato starch wastewater precipitates, so that waste (potato residues) generated in a potato starch processing plant is further utilized to fire biochar to serve as a microbial carrier to prepare a microbial agent, and the microbial agent can be quickly adapted to the environment and can serve as a potato starch wastewater treatment microbial agent for use as soon as the environment is suitable for use.
The invention adopts the following technical scheme:
1. bacillus bailii Bacillus velezensis GAU, deposited at the China collection culture Collection, address: in the eight-channel 299-grade university of Wuhan in Wuhan district of Hubei province, the preservation number is CCTCC M20211501.
2. An application of bacillus bailii Bacillus velezensis GAU35 in potato starch wastewater treatment.
3. A method of screening for isolation of bacillus beljalis Bacillus velezensis according to claim 1, comprising: (1) sample collection; (2) Separating microorganisms in activated sludge from a potato starch waste processing plant; (3) strain culture and growth capacity detection; (4) determining the capability of bacteria to degrade potato starch waste water; identification of the strain (5).
4. A method for preparing a wastewater treatment microbial inoculum is characterized by comprising the following steps:
(1) Firing biochar immobilization carrier
Collecting potato residue generated in the potato starch production process, drying at 80 ℃ in an oven, crushing, and sieving with a 0.20mm standard sieve. And (3) placing a certain amount of weighed samples into a ceramic boat, placing the ceramic boat into a tube furnace, carrying out constant-temperature pyrolysis on the burned biochar at the temperature of 550 ℃, 350 ℃, 450 ℃,550 ℃, 650 ℃, 750 ℃ and 850 ℃ respectively at the heating rate of 10 ℃/min under the protection of nitrogen, naturally cooling to room temperature, obtaining the product, namely the biochar, and weighing and calculating the yield. The temperature for firing the biochar is 550 ℃, 350 ℃, 450 ℃,550 ℃, 650 ℃, 750 ℃ and 850 ℃ respectively. The figure shows that the charcoal fired at 250 ℃ has the highest yield, but is not available because the charcoal is reddish brown and is not completely fired, and the charcoal fired at 550 ℃ has stable yield, so that the charcoal fired at 550 ℃ is adopted in the experiment from the aspects of energy and time saving. Grinding biochar in a mortar, sieving with a 0.20mm standard sieve to obtain biochar with micrometer particle size, soaking in 5% hydrochloric acid for 8 hr, soaking in distilled water for 8 hr, washing with distilled water to neutrality, and oven drying at 80deg.C to obtain potato residue biochar.
(2) Adding the strain according to claim 1 and culturing
1g of biochar is weighed and placed in a 250mL conical flask for autoclaving at 121 ℃ for 20min. Then 40mL of the prepared bacterial suspension is added into a 250mL conical flask, the mixture is gently shaken until biochar is dispersed in the suspension, the mixture is placed into a constant temperature shaking table (25 ℃ C., 180 rpm) for culturing for 12 hours, and the bacterial content in the suspension is measured every two hours. As shown in FIG. 6, the charcoal immobilization time of the strain was measured. Pouring the immobilized microorganism bacterial suspension into a 50mL centrifuge tube, centrifuging at 5000r/min for 10min, removing supernatant after centrifugation, and freeze-drying. The frozen and dried biochar immobilized microorganism powder is stored in a refrigerator at 4 ℃.
(3) Performance analysis of microbial agent for degrading potato starch wastewater
Preparation of strains: strain GAU35 was prepared as a bacterial suspension. And adsorbing and fixing the bacterial suspension and the biochar for about 8 hours. The immobilized microorganism is prepared into a solid microbial inoculum by a freeze drying method. The immobilized microbial agent is applied to starch wastewater with the COD concentration of about 2500mg/L, and compared with the non-immobilized GAU35 and the non-bacteria added biochar, the immobilized microbial agent prepared by the method has a good effect in the aspect of removing the COD of potato starch wastewater. The charcoal prepared by the method is used for immobilizing the bacterial strain GAU35 isolated by the method, so that the bacterial strain can be quickly adapted to the environment and can be used for efficiently treating potato starch wastewater.
The beneficial effects are that: under pure culture conditions, the strain GAU35 has obvious degradation effect on COD and TOC of potato starch wastewater, and according to FIG. 2, after the potato starch wastewater with the initial COD concentration of 2000mg/L is degraded by GAU35 for 12 hours, the COD and the TOC are obviously reduced, the COD is reduced from 2200mg/L to 1400mg/L, and the TOC is reduced from 2000mg/L to 1200mg/L. Compared with pure culture, the microbial agent has more obvious potato starch wastewater treatment effect. According to FIG. 7, the COD removal efficiency of the microbial agent (GAU35+Biochar) in the same potato starch wastewater can be 50% or more in a short time, and the treatment effect can be 70% or more with the increase of the treatment time. The carrier material used in the preparation process of the microbial inoculum is potato residue, is a cheap and environment-friendly material in terms of social benefit and environmental benefit, and has a certain adsorption effect on wastewater by biochar. The invention combines the double treatment effects of the biochar and the microorganism on the potato starch wastewater, and is more environment-friendly and efficient than the traditional starch wastewater treatment method.
Drawings
FIG. 1 is a graph showing the growth of strain GAU 35.
The growth of the strain GAU35 in the LB basal medium with the culture temperature of 25 ℃ and the rotation speed of 180rpm can be seen from the graph, and the strain can grow logarithmically when cultured for 12 hours, which indicates that the strain can grow rapidly under the culture conditions.
FIG. 2 shows the degradation capacity of the strain GAU35 for potato starch wastewater.
Wherein, FIG. 2 shows that the COD and TOC content in potato starch wastewater are continuously reduced along with the backward movement of the culture time by measuring the change of COD and TOC in potato starch wastewater before and after GAU35 treatment, and the COD and TOC content in potato starch wastewater is obviously reduced after 12 hours of culture, which indicates that the strain GAU35 has the capability of treating potato starch wastewater
FIG. 3 is a diagram showing the exploration of the conditions for the preparation of biochar.
Wherein, fig. 3A is a search for the charcoal-firing temperature, and according to the research result, as the charcoal-cracking temperature is continuously increased, the charcoal yield approaches to be stable, and at the temperature of 550 ℃, the charcoal yield is basically stable, which means that the ash content of the charcoal fired at the temperature is completely removed, and the production standard of the charcoal is reached. FIG. 3B shows that the microorganism is immobilized by using the biochar prepared at different temperatures, and the result shows that the adsorption efficiency of the microorganism is highest after the temperature of preparing the biochar reaches 550 ℃. Therefore, in summary, 550 ℃ is the optimal temperature for preparing the potato residue biochar.
FIG. 4 is a flow chart of the baking of potato residue biochar.
The preparation and firing process of the biochar mainly comprises three parts, namely pretreatment of potato residues, wherein potato residues generated after starch production contain a large amount of water, and the particles are large and cannot be directly used for the firing of the biochar, and the baking, crushing and sieving are needed. In order to ensure that the biological charcoal of potato residue has a certain aperture and micropore number after firing, the potato residue is further pretreated, and zinc chloride with a certain concentration is used for pretreatment, so that the micropore number is kept in the biological charcoal forming process. The final step is to clean the cracked biochar to remove some impurities and obtain pure biochar.
FIG. 5 shows the morphology of organisms in different fields of view.
Wherein, fig. 5a shows that the biochar under the visual field of eyes is pure black, without brown and grey-white substances, and the cracking process of the biochar is completed under the completely anaerobic condition. Fig. 5b shows that the biochar is in an irregular, loose state, not a compact, packed particle, as can be seen under an optical microscope, demonstrating the potential of the biochar prepared in this state to adsorb microorganisms and other organics. Fig. 5c shows that the biochar has many mesopores through scanning electron microscopy, so that the adsorption potential of the biochar is more fully demonstrated.
FIG. 6 shows the immobilization time of strain GUA 35.
The prepared biochar is used for adsorbing and fixing strains GAU35 with different initial concentrations, and experimental results show that the immobilization time of the biochar on the strains with different concentrations is basically consistent. From FIG. 6 we can see that the immobilization time of the potato residue biochar to the strain GAU35 was substantially stabilized around 8h, regardless of the initial concentration. After 8 hours, the adsorption of the biochar to the microorganisms is saturated and cannot be changed, and from the fact that the immobilization time of the prepared potato residue biochar to the strain GAU35 is about 8 hours can be confirmed.
FIG. 7 shows the degradation capacity of the immobilized microbial agent on potato starch wastewater.
The immobilized microbial agent has certain potential and efficiency in the aspect of removing the COD of the potato starch wastewater, and the prepared immobilized microbial agent has stronger degradation capability on the potato starch wastewater at 24 hours.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the following detailed description of the embodiments of the present invention will be given with reference to the accompanying drawings. Examples of these preferred embodiments are illustrated in the accompanying drawings. The embodiments of the invention shown in the drawings and described in accordance with the drawings are merely exemplary and the invention is not limited to these embodiments. It should be noted here that, in order to avoid obscuring the technical solution of the present invention due to unnecessary details, only the structures and/or processing steps closely related to the solution according to the present invention are shown in the drawings, while other details having little relation are omitted.
Example 1
The embodiment provides a strain capable of efficiently degrading potato starch wastewater, wherein the strain is Bacillus velezensis GAU (GAU is English abbreviation of Gansu agricultural university, 35 is serial number), and the strain is preserved in China Center for Type Culture Collection (CCTCC) at 11 months and 29 days 2021, addresses: in the eight-path 299-grade university of Wuhan in Wuhan, hubei province, the preservation number is CCTCC M20211501, and the preservation proves that the time is 2021, 12 months and 6 days.
Example 2
The embodiment provides a method for separating potato starch wastewater treatment strains, which comprises the following steps:
1. sludge for collecting sewage outlet of potato starch processing plant
Soil samples were taken from potato starch processing plants located in the city of the firm western city of Gansu province. The plant elevation was 1889.7m. Immediately after sample collection, the sample is put into a sterile sealing bag and placed in a vehicle-mounted refrigerator, and is stored at-20 ℃ for standby after being transported to a laboratory, and sediment samples in the sample are experimentally selected for separation research.
2. Culturing and separating purified strain
(1) 30g of soil sample is weighed and quickly poured into a 500mL triangular flask filled with 100mL of sterile water, and the mixture is oscillated for 5-10 min to fully break up the soil sample, so as to prepare soil suspension. 200mL of LB liquid medium sucked by a sterile pipette is added into the soil fungus suspension, and enrichment culture is carried out for 36h at 15 ℃.
(2) Configuration 10 -2 、10 -4 、10 -6 、10 -8 Diluting the bacterial liquid, sucking 100 mu l of bacterial liquid and dripping the bacterial liquid onto the surface of a culture medium. Respectively coating bacterial solutions with different dilution concentrations on separation culture mediums with different nitrogen sources, respectively placingCulturing at 5deg.C, 10deg.C, 15deg.C and 20deg.C in incubator upside down, and picking single strain.
(3) Preliminary identification of isolated strains
Mu.l of bacterial liquid is sucked up, the supernatant is removed by centrifugation in a sterilized 1.5ml centrifuge tube prepared in advance, genomic DNA is extracted by using a bacterial DNA extraction kit, and the sample is sent for sequencing. And submitting the sequencing result to a GenBank database through a Blast program for similarity comparison search, and determining the strains screened by the related genus and species kindred strain types.
3. Testing degradation performance of functional strain on potato starch wastewater
According to the taxonomic status of the isolated strains, 1-2 strains are selected from each genus to verify the degradation efficiency of potato starch wastewater.
Example 3
The embodiment example provides a method for manufacturing a microbial agent, which comprises the following steps:
1. firing biochar
Collecting potato residue generated in the potato starch production process, drying at 80 ℃ in an oven, crushing, and sieving with a 0.20mm standard sieve. And (3) placing a certain amount of weighed samples into a ceramic boat, placing the ceramic boat into a tube furnace, carrying out constant-temperature pyrolysis on the burned biochar at the temperature of 550 ℃, 350 ℃, 450 ℃,550 ℃, 650 ℃, 750 ℃ and 850 ℃ respectively at the heating rate of 10 ℃/min under the protection of nitrogen, naturally cooling to room temperature, obtaining the product, namely the biochar, and weighing and calculating the yield. As shown in FIG. 3A, the temperature of the calcined charcoal was 550 ℃, 350 ℃, 450 ℃,550 ℃, 650 ℃, 750 ℃ and 850 ℃, respectively. The figure shows that the charcoal fired at 250 ℃ has the highest yield, but is not available because the charcoal is reddish brown and is not completely fired, and the charcoal fired at 550 ℃ has stable yield, so that the charcoal fired at 550 ℃ is adopted in the experiment from the aspects of energy and time saving. Grinding biochar in mortar, sieving with 0.20mm standard sieve to obtain biochar with micrometer particle diameter, soaking in 5% hydrochloric acid for 8 hr, soaking in distilled water for 8 hr, washing with distilled water to neutrality, and oven drying at 80deg.C to obtain potato biochar, the flow chart is shown in figure 4.
2. Microbial agent for preparing and treating potato starch wastewater
1g of biochar is weighed and placed in a 250mL conical flask for autoclaving at 121 ℃ for 20min. Then 40mL of the prepared bacterial suspension is added into a 250mL conical flask, the mixture is gently shaken until biochar is dispersed in the suspension, the mixture is placed into a constant temperature shaking table (25 ℃ C., 180 rpm) for culturing for 12 hours, and the bacterial content in the suspension is measured every two hours. As shown in FIG. 6, the charcoal immobilization time of the strain was measured. Pouring the immobilized microorganism bacterial suspension into a 50mL centrifuge tube, centrifuging at 5000r/min for 10min, removing supernatant after centrifugation, and freeze-drying. The frozen and dried biochar immobilized microorganism powder is stored in a refrigerator at 4 ℃.
Example 4
The embodiment provides an application of a potato starch wastewater treatment microbial inoculum, which comprises the following steps:
the biological carbon immobilized strain GAU35 is prepared from the potato residues, and the degradation efficiency of the COD of the potato starch wastewater is improved. Preparation of the strain (1), the strain GAU35 was prepared as a bacterial suspension. And (2) adsorbing and fixing the bacterial suspension and the biochar for about 8 hours. (3) The immobilized microorganism is prepared into a solid microbial inoculum by a freeze drying method. (4) The immobilized microbial agent is applied to starch wastewater with the COD concentration of 2500mg/L, and compared with the non-immobilized GAU35 and the non-bacteria added biochar, the immobilized microbial agent used by us has certain potential and efficiency in the aspect of removing the COD of the potato starch wastewater, and the bacterial strain GAU35 separated by us is immobilized by the prepared biochar, so that a biological method is fast suitable for the environment and high-efficiency potato starch wastewater treatment is performed.
The foregoing is merely exemplary of the application and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the application and are intended to be comprehended within the scope of the application.

Claims (2)

1. Bacillus beleiensis strain 35, characterized in that the strain was deposited in the chinese collection culture collection at 2021, 11/29, address: in the eight-channel 299-grade university of Wuhan in Wuhan district of Hubei province, the preservation number is CCTCCM20211501.
2. The use of bacillus beleiensis bacillus dysonsgau 35 according to claim 1 in potato starch wastewater treatment.
CN202210010239.1A 2022-01-06 2022-01-06 Potato starch wastewater treatment microbial inoculum Active CN115011499B (en)

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Publication number Priority date Publication date Assignee Title
CN103627654B (en) * 2013-10-17 2015-08-26 北京华利嘉环境工程技术有限公司 A kind of starch degradation bacterium and the application in water treatment thereof
CN112094775B (en) * 2020-09-22 2022-07-26 中国石油天然气集团有限公司 Bacillus belgii and screening culture method and application thereof
CN113249278A (en) * 2021-07-05 2021-08-13 天津北洋百川生物技术有限公司 Microbial agent for treating starch wastewater

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