CN102120976B - Sphingobium yanoikuyae and application thereof in degrading polycyclic aromatic hydrocarbon - Google Patents
Sphingobium yanoikuyae and application thereof in degrading polycyclic aromatic hydrocarbon Download PDFInfo
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Abstract
The invention discloses a Sphingobium yanoikuyae and an application thereof in degrading polycyclic aromatic hydrocarbon. The Sphingobium yanoikuyae provided by the invention is named as LD29 with the strain collection number of CGMCC No.4400. The Sphingobium yanoikuyae LD 29 can grow and breed by respectively taking phenanthrene, anthracene, fluoranthene and pyrene as the only carbon source and energy source under the aerobic condition. In addition, the Sphingobium yanoikuyae LD 29 has a degrading effect on mixed polycyclic aromatic hydrocarbon, especially on pentacyclic benzopyrene [alpha] in the mixed polycyclic aromatic hydrocarbon. The Sphingobium yanoikuyae can be used for repairing soil and the water body polluted by the polycyclic aromatic hydrocarbon, and is quick, convenient and environmentally-friendly, and can be used for reducing the content of the polycyclic aromatic hydrocarbon in the environment, thereby providing new microorganism resources for bioremediation for polycyclic aromatic hydrocarbon pollution, and having an important value for environmental governance as well as considerable economic benefit and good social benefit.
Description
Technical field
The invention belongs to environmental pollutant biologic treating technique field, relate to a strain and vow Noguchi Sphingobacterium and the application in degrading polycyclic aromatic hydrocarbons thereof.
Background technology
Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons is called for short PAHs) is the aromatic hydrocarbons that contains two or more phenyl ring.Under the normal temperature; Pure polycyclic aromatic hydrocarbons is solid-state, and its general characteristic is HMP, high boiling point, low water solubility, and the water-soluble of polycyclic aromatic hydrocarbons reduces along with the increase of phenyl ring quantity; Its bioavailability and degradability also decrease, and the time that in environment, stops is long.
Polycyclic aromatic hydrocarbons is from nature and mankind's activity.Polycyclic aromatic hydrocarbons is a type organic that just was present in occurring in nature originally; It is synthetic in the aerobic growth process to pass through some algae, plant and degradation bacteria; Also possibly be that mikrobe produces polycyclic aromatic hydrocarbons when under anaerobic conditions, degrading large amount of organic; Such as all containing a large amount of polycyclic aromatic hydrocarbonss in unquarried coal and the oil, on the other hand, organic incomplete combustion is to produce a polycyclic aromatic hydrocarbons contaminated important factor.Polycyclic aromatic hydrocarbons in the physical environment does not constitute too big threat to environment, and has been enough to influence the safety of whole ecological system from the content of the polycyclic aromatic hydrocarbons of mankind's activity.
Along with the aggravation of mankind's activity, the pollution of venomous injurant confrontation environment increases the weight of day by day, and wherein polycyclic aromatic hydrocarbons is exactly the objectionable impurities of a quasi-representative, gets more and more people's extensive concerning because of it has teratogenecity, carinogenicity and mutagenicity.Polycyclic aromatic hydrocarbons all has distribution in atmosphere, water, soil and plant, though the polycyclic aromatic hydrocarbons concentration in the atmosphere is not very high, it can damage the lung of animal through respiration; Polycyclic aromatic hydrocarbons in the water body is through polycyclic aromatic hydrocarbons sedimentation in the atmosphere, and the modes such as direct discharging that contain polycyclic aromatic hydrocarbons sewage get in the middle of the water body, and the content of the polycyclic aromatic hydrocarbons in the water body deposit is very high; Polycyclic aromatic hydrocarbons in the soil is mainly derived from atmospheric particles settling and precipitation snowfall, also has some directly toppling over and blowdown from oil field and depleted refinery; Polycyclic aromatic hydrocarbons major part in the plant derives from atmospheric precipitation, and some plants can absorb this type material.Polycyclic aromatic hydrocarbons is distributed in our living environment widely, and human body and environment are all had very big harm, therefore, is to have very much practical significance to polycyclic aromatic hydrocarbons contaminated biological prosthetic research.
Although polycyclic aromatic hydrocarbons can pass through methods such as absorption, volatilization, photodissociation, chemical degradation and remove, biological degradation is most important degradation pathway.At present, scholars have isolated many mikrobes that can degrading polycyclic aromatic hydrocarbons, and wherein bacterium in the highest flight.But the mikrobe of the aromatic hydrocarbons of in the past degrading all is to low-molecular-weight polycyclic aromatic hydrocarbons mostly, and is not obvious to the degradation effect of polycyclic aromatic hydrocarbon with high molecular weight.Therefore, seeking degraded macromolecular amount (epipodium) polycyclic aromatic hydrocarbon-degrading bacteria is present hot issue.
Summary of the invention
The purpose of this invention is to provide a strain and vow Noguchi Sphingobacterium and the application in degrading polycyclic aromatic hydrocarbons thereof.
Arrow Noguchi Sphingobacterium provided by the invention (Sphingobium yanoikuyae); Called after LD29; Separation is No. 1 well oil outlet of U.S. platform crude oil pollution top layer (＜2cm) sandy soil from oil field, Fushan, Haikou City, Hainan Province; (be called for short CGMCC, the address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), culture presevation number is CGMCC No.4400 to be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on December 03rd, 2010.Vow that Noguchi Sphingobacterium (Sphingobium yanoikuyae) LD29CGMCC No.4400 is called for short arrow Noguchi Sphingobacterium LD29.
Vow that Noguchi Sphingobacterium LD29 can be used for the organic aromatic compound of degrading.Said organic aromatic compound can be polycyclic aromatic hydrocarbon compounds (polycyclic arene compound).Said polycyclic aromatic hydrocarbon compounds (polycyclic arene compound) specifically can be at least a in phenanthrene, anthracene, fluoranthene, pyrene and benzo [a] pyrene.
The present invention also protects a kind of preparation (microbial inoculum) of the organic aromatic compound of degrading, and its activeconstituents is for vowing Noguchi Sphingobacterium LD29.Said organic aromatic compound can be polycyclic aromatic hydrocarbon compounds (polycyclic arene compound).Said polycyclic aromatic hydrocarbon compounds (polycyclic arene compound) specifically can be at least a in phenanthrene, anthracene, fluoranthene, pyrene and benzo [a] pyrene.
Vow that Noguchi Sphingobacterium LD29 can be used for the environmental pollution reparation.Said environmental pollution can be organic aromatic compound and pollutes.Said organic aromatic compound can be polycyclic aromatic hydrocarbon compounds (polycyclic arene compound).Said polycyclic aromatic hydrocarbon compounds (polycyclic arene compound) specifically can be at least a in phenanthrene, anthracene, fluoranthene, pyrene and benzo [a] pyrene.Said environment is soil or water body.
Vow Noguchi Sphingobacterium LD29 can be under aerobic condition respectively with phenanthrene, anthracene, fluoranthene and pyrene as sole carbon source and energy growth and breeding, simultaneously with its degraded.In addition, vow that Noguchi Sphingobacterium LD29 also has degradation effect to mixing polycyclic aromatic hydrocarbons, has especially also produced degradation effect to benzo [a] pyrene that mixes five rings in the polycyclic aromatic hydrocarbons.The present invention can be used for the reparation of polycyclic aromatic hydrocarbon pollution and the reparation of polycyclic aromatic hydrocarbons contaminated water body; Reduce polycyclic aromatic hydrocarbon content in the environment; Has advantage quick, convenient, environmental protection; For polycyclic aromatic hydrocarbons contaminated biological prosthetic provides new Microbial resources, have great value for environmental improvement, have considerable economic and good social benefit.
Description of drawings
Fig. 1 is for vowing that Noguchi Sphingobacterium LD29 is to mixing PAHs degradation effect graphic representation.
Fig. 2 is that different glucose concn are to vowing the histogram that influences of Noguchi Sphingobacterium LD29 degraded fluoranthene; CK is not for adding the processing of glucose mother liquid.
Fig. 3 is that different nitrogen concentration is to vowing the histogram that influences of Noguchi Sphingobacterium LD29 degraded fluoranthene; CK is not for adding the processing of peptone mother liquor.
Fig. 4 is the typical curve of luxuriant and rich with fragrance standard substance.
Fig. 5 is the typical curve of anthracene standard substance.
Fig. 6 is the typical curve of fluoranthene standard substance.
Fig. 7 is the typical curve of pyrene standard substance.
Fig. 8 is the typical curve of benzo [a] pyrene standard substance.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique among the following embodiment like no specified otherwise, is ordinary method.Used test materials among the following embodiment like no specified otherwise, is to buy from routine biochemistry reagent shop and obtains.
The detection method of phenanthrene, anthracene, fluoranthene, pyrene and benzo [a] pyrene:
Experiment adds 10mL ETHYLE ACETATE, vortex 30s after finishing in each test tube.Leave standstill and treat that water layer separates with organic layer, with transfer pipet from the upper strata organic layer get 1mL ETHYLE ACETATE to clean Brown Glass Brown glass bottles and jars only.Blow ETHYLE ACETATE gently to volatilization fully with nitrogen gas stream under 55 ℃, add the dissolving crystallized PAHs of 2mL acetonitrile again., in another clean Brown Glass Brown glass bottles and jars only, close the lid with the organic phase membrane filtration acetonitrile solution in 0.22 μ m aperture.Measure polycyclic aromatic hydrocarbons concentration with Agilent LC1200 high performance liquid chromatograph, adopt Venusil MP-C18 post (particle diameter 5 μ m, diameter 4mm, length 250mm, Tianjin Beaune Ai Jieer science and technology), moving phase is acetonitrile and water; Adopt 60% acetonitrile wash-out in the preceding 5min, at ensuing 25min, carry out gradient elution (60% acetonitrile → 100% acetonitrile), 100% acetonitrile wash-out keeps 5min, amounts to 35min.
The typical curve of luxuriant and rich with fragrance standard substance is seen Fig. 4, and target peak is for to begin 24.157 ± 0.059min from wash-out.
The typical curve of anthracene standard substance is seen Fig. 5, and target peak is for to begin 24.579 ± 0.076min from wash-out.
The standard song of fluoranthene standard substance is seen Fig. 6, and target peak is for to begin 25.801 ± 0.055min from wash-out.
The typical curve of pyrene standard substance is seen Fig. 7, and target peak is for to begin 26.311 ± 0.056min from wash-out.
The typical curve of benzo [a] pyrene standard substance is seen Fig. 8, and target peak is for to begin 29.700 ± 0.060min from wash-out.
Separation and the evaluation of embodiment 1, arrow Noguchi Sphingobacterium LD29
One, sample collecting
No. 1 well oil outlet of the U.S. platform in oil field, Fushan, in October, 2008 collection in worksite Haikou City, Hainan Province crude oil pollution top layer (＜2cm) sandy soil.
Two, vow separation and the screening of Noguchi Sphingobacterium LD29
Adopt water-silicone oil diphasic system domestication polycyclic aromatic hydrocarbon-degrading bacteria, the initial concentration of anthracene is 100mg/L.Water minimal medium (pH 7.5): MgSO
47H
2O 0.2g, KH
2PO
41.0g, K
2HPO
41.0g, FeSO
40.05g, CaCl
20.02g, NH
4NO
31.0g, NaCl 0.5g, deionized water 1000mL.
Passing through some generations tames repeatedly; Streak culture in the NR solid medium, obtain the pure bacterium of many strains, pure bacterium is through being seeded to its degradation capability of checking in the liquid nutrient medium; Obtain can the degrade efficient degrading bacteria of anthracene, phenanthrene, pyrene, fluoranthene of a strain at last, with its called after LD29.NR solid medium (pH7.4～7.6): Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium-chlor 5g, agar 15g～20g, deionized water 1000mL.
Three, vow the evaluation of Noguchi Sphingobacterium LD29
1, morphology is identified
It is circular that bacterium colony is rule, yellow, and surface wettability, smooth, projection, neat in edge, opaque, the quality thickness.
2, Physiology and biochemistry is identified
Qualification result is seen table 1.
The physiological and biochemical property of table 1 bacterial strain LD29
| Test subject | The result | Test subject | The result | Test subject | The result | Test subject | The result |
| Cellular form | Shaft-like | Gramstaining | Negative | Oxydase | + | Catalase | + |
| Contrast | - | The D-melibiose | + | The p-phenylglycollic acid | - | The L-Histidine | - |
| The a-Schardinger dextrins | + | Methyl glucoside | + | Methylene-succinic acid | - | Ls-hydroxyproline | + |
| Dextrin | + | The D-psicose | + | The a-ketobutyric acid | - | The L-leucine | + |
| Glycogen | - | The D-raffinose | + | The a-keto-glutaric acid | - | The L-ornithine | - |
| Tween40 | + | The L-rhamnosyl | + | The a-oxopentanoic acid | - | The L-phenylalanine(Phe) | - |
| Tween80 | + | The D-sorbyl alcohol | - | D, L-lactic acid | B | The L-proline(Pro) | + |
| N-acetylgalactosamine | - | Sucrose | + | Propanedioic acid | - | The L-Pyrrolidonecarboxylic acid | - |
| The N-acetylglucosamine | + | The D-trehalose | + | Propionic acid | + | The D-Serine | - |
| Ribitol | - | Turanose | + | Quinic acid | + | The L-Serine | - |
| L-arabinose | + | Xylitol | - | The D-saccharinic acid | - | The L-Threonine | - |
| The D-arabitol | - | Pyruvic Acid Methyl ester | + | The certain herbaceous plants with big flowers diacid | - | D, the L-carnitine | - |
| The D-cellobiose | + | Succinic Acid one methyl esters | - | Succinic Acid | + | The g-propalanine | - |
| The i-tetrahydroxybutane | - | Acetate | + | Bromosuccinic acid | + | Urocanic acid | - |
| D-fructose | + | Suitable-aconic acid | - | Succinamic acid | - | Inosine | - |
| L-fructose | - | Hydrocerol A | - | Glucuronamide | - | Uridine | - |
| The D-semi-lactosi | + | Formic acid | - | The L alanimamides | + | Thymidine | - |
| Gentiobiose | + | The D-galactonolactone | - | The D-L-Ala | - | Phenyl-ethyl amine | - |
| A-D-glucose | + | The D-galacturonic acid | - | The L-L-Ala | - | Putrescine | - |
| The m-inositol | - | The D-glyconic acid | + | The L-alanyl-glycine | + | The 2-monoethanolamine | - |
| The a-D-lactose | + | The b-methyl glucoside | - | Altheine | - | 2, the 3-butyleneglycol | - |
| Lactulose | + | The D-glucuronic acid | - | The L-aspartic acid | - | Glycerine | - |
| SANMALT-S | + | The a-hydroxybutyric acid | L-L-glutamic acid | + | D, the L-glycerophosphate | - | |
| D-N.F,USP MANNITOL | - | The b-hydroxybutyric acid | + | The glycyl aspartic acid | + | The D-glucose-1-phosphate | - |
| The D-seminose | + | The g-hydroxybutyric acid | - | Glycyl L-glutamic acid | + | The D-Robison ester | - |
3,16SrDNA identifies
The 16SrDNA sequence is seen the sequence 1 of sequence table.
Form, physiological and biochemical property and 16SrDNA sequence through to bacterial strain are carried out determination and analysis, LD29 is accredited as vows Noguchi Sphingobacterium (Sphingobium yanoikuyae).To vow that Noguchi Sphingobacterium (Sphingobiumyanoikuyae) LD29 is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is No.4400.Vow that Noguchi Sphingobacterium (Sphingobium yanoikuyae) LD29CGMCC No.4400 is called for short arrow Noguchi Sphingobacterium LD29.The suitable growth condition of vowing Noguchi Sphingobacterium LD29 is: pH=7.5,30 ℃ of temperature.
Content * 100% of polycyclic arene compound before the content ÷ inoculation of residual rate=cultivation back polycyclic arene compound.Content * 100% of polycyclic arene compound before degradation efficiency=(content of the content of polycyclic arene compound before the inoculation-cultivation back polycyclic arene compound) ÷ inoculation.Degradation efficiency+residual rate=1.
The water minimal medium is with embodiment 1.
Picking is vowed the single bacterium colony of Noguchi Sphingobacterium LD29, in the water minimal medium of pH=7.5, cultivates 2 days for 30 ℃, obtains vowing Noguchi Sphingobacterium LD29 bacterium liquid.
One, vows the degraded of Noguchi Sphingobacterium LD29 to the simplification compound
1mL vows that Noguchi Sphingobacterium LD29 bacterium liquid is seeded in the water minimal medium that 10mL contains the various polycyclic arene compounds of 50mg/L (phenanthrene, anthracene, fluoranthene or pyrene); At 180r/min; Lucifuge shaking culture in 30 ℃ of shaking tables; Measure the residual rate of polycyclic arene compound after 4 days, calculate degradation efficiency.
Carry out repeated experiments three times, results averaged.Luxuriant and rich with fragrance degradation efficiency is 92%, and the degradation efficiency of anthracene is 87%, and the degradation efficiency of fluoranthene is 28%, and the degradation efficiency of pyrene is 7%.
Two, vow that Noguchi Sphingobacterium LD29 is to mixing the degraded of polycyclic aromatic hydrocarbons
Get 6 groups of test tubes, 3 every group; In every test tube; 1mL is vowed that Noguchi Sphingobacterium LD29 bacterium liquid is seeded to 10mL and contains in the polycyclic arene compound water minimal medium of (containing each 100mg/L of phenanthrene, anthracene, fluoranthene and pyrene, benzo [a] pyrene 10mg/L), at 180r/min; Lucifuge shaking culture in 30 ℃ of shaking tables; Take a sample (get 1 group of test tube every day, the result is the MV of 3 test tubes) every day, measures the residual rate (see figure 1) of various polycyclic arene compounds.Calculate degradation efficiency after 6 days.
Vow that Noguchi Sphingobacterium LD29 has quick degradation capability to the phenanthrene and the anthracene of 3 rings, just had nearly 11% phenanthrene and 45% anthracene be degraded on the 1st day.After 6 days, the phenanthrene of 3 rings and the residual quantity of anthracene are respectively 0.5mg/L and 38mg/L.The fluoranthene of 4 rings and the residual quantity of pyrene are respectively 41mg/L and 52mg/L.In the time of the 6th day, luxuriant and rich with fragrance degradation efficiency is 99%, and the degradation efficiency of anthracene is 62%, and the degradation efficiency of fluoranthene is 59%, and the degradation efficiency of pyrene is 48%, and the degradation efficiency of benzo [a] pyrene is 17%.The mixing polycyclic aromatic hydrocarbons of vowing that Noguchi Sphingobacterium LD29 degradable phenanthrene, anthracene, fluoranthene, pyrene and benzo [a] pyrene are formed is described, and polycyclic aromatic hydrocarbon with high molecular weight is also had good degradation effect.
The water minimal medium is with embodiment 1, and Different Nitrogen Concentration is 350mg/L.
Degradation rate=(C
0-C
1)/C
0* 100%.Residual rate=C
1/ C
0* 100%.Degradation rate+residual rate=1.
Picking is vowed the single bacterium colony of Noguchi Sphingobacterium LD29, in the water minimal medium of pH=7.5, cultivates 2 days for 30 ℃, obtains vowing Noguchi Sphingobacterium LD29 bacterium liquid.
One, adds glucose to vowing the influence of Noguchi Sphingobacterium LD29 fluoranthene degradation rate
1, disposes the glucose mother liquid (glucose content is 1000mg/L) of 1000mg/L with deionized water, 115 ℃ of sterilization 20min.
2, prepare 5 test tubes; Wherein 4 test tubes add the glucose mother liquid after 0.5mL, 1.0mL, 2.0mL, the 5.0mL sterilization respectively; The water minimal medium that in 5 test tubes, adds fluoranthene and sterilization then respectively adds 1mL then and vows Noguchi Sphingobacterium LD29 bacterium liquid in each test tube.The final volume of 5 test tubes is 10mL, and the final concentration of fluoranthene is 100mg/L (C
0=100mg/L), the glucose final concentration is respectively 0mg/L, 50mg/L, 100mg/L, 200mg/L, 500mg/L.
3, test tube tilts to place 30 ℃ of shaking tables, lucifuge, 180r/min shaking culture 6d, detects the concentration (C of fluoranthene
1).
Carry out repeated experiments three times, results averaged.The detected result of residual rate is seen Fig. 2.Degradation rate is 34% when not adding glucose.Along with glucose concn is increased to 200mg/L from 0mg/L (CK); Vow that Noguchi Sphingobacterium LD29 also increases by 69% from 34% gradually to the degradation rate of fluoranthene; And be in the processing of 500mg/L at glucose concn, the degradation rate of fluoranthene on the contrary than 200mg/L handle low.An amount of glucose is added in this explanation in the degraded system, can promote the degraded to fluoranthene, but the promoter action of glucose can only increase progressively in the finite concentration scope, then restraining effect can occur when surpassing certain limit.
Two, add peptone to vowing the influence of Noguchi Sphingobacterium LD29 fluoranthene degradation rate
1, takes by weighing 0.138g peptone (nitrogen content is 14.5%), be dissolved in the 20mL deionized water, obtain peptone mother liquor (Different Nitrogen Concentration is 1000mg/L).
2, prepare 6 test tubes, wherein 5 test tubes add the peptone mother liquor respectively, in 6 test tubes, add the water minimal medium of fluoranthene and sterilization then respectively, in each test tube, add 1mL then and vow Noguchi Sphingobacterium LD29 bacterium liquid.The final volume of 5 test tubes is 10mL, the final concentration C of fluoranthene
0Be 100mg/L, the nitrogen final concentration is respectively 350mg/L, 360mg/L, 370mg/L, 400mg/L, 450mg/L, 500mg/L.
3, test tube tilts to place 30 ℃ of shaking tables, lucifuge, 180r/min shaking culture 6d, detects the concentration (C of fluoranthene
1).
Carry out repeated experiments three times, results averaged.The detected result of residual rate is seen Fig. 3.Degradation rate is 34% when not adding peptone.Add the nitrogen peptone and can promote to vow the degraded of Noguchi Sphingobacterium LD29 fluoranthene; Along with Different Nitrogen Concentration is increased to 400mg/L from 350mg/L; Vow that Noguchi Sphingobacterium LD29 is increased to 81% to the degradation rate of fluoranthene from 34% fast, the increase of nitrogen is then played restraining effect to the degraded of fluoranthene on the contrary.
Claims (4)
1. vow Noguchi Sphingobacterium (Sphingobium yanoikuyae) LD29, its deposit number is CGMCC No.4400.
2. vow the application of Noguchi Sphingobacterium (Sphingobium yanoikuyae) LD29 CGMCC No.4400 in the degraded organic aromatic compound;
Said organic aromatic compound is at least a in phenanthrene, anthracene, fluoranthene, pyrene and benzo [a] pyrene.
3. the preparation of the organic aromatic compound of degrading, its activeconstituents is for vowing Noguchi Sphingobacterium (Sphingobium yanoikuyae) LD29 CGMCC No.4400;
Said organic aromatic compound is at least a in phenanthrene, anthracene, fluoranthene, pyrene and benzo [a] pyrene.
4. vow the application of Noguchi Sphingobacterium (Sphingobium yanoikuyae) LD29 CGMCC No.4400 in environmental pollution is repaired;
Said pollution is that organic aromatic compound pollutes; Said organic aromatic compound is at least a in phenanthrene, anthracene, fluoranthene, pyrene and benzo [a] pyrene; Said environment is soil or water body.
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| CN103060419A (en) * | 2012-12-25 | 2013-04-24 | 天津大学 | Synergistic effect of multiple bacterial strains on degradation of polyphenol pollutants and research method thereof |
| CN104046580B (en) * | 2014-05-23 | 2017-06-20 | 华中农业大学 | Sphingobacterium bacterial strain and its application for degrading polycyclic aromatic hydrocarbons class organic pollution |
| CN104830942A (en) * | 2015-05-11 | 2015-08-12 | 汪周启 | Biological alcohol production technique of organic sewage |
| CN105199988B (en) * | 2015-09-29 | 2018-07-13 | 南京农业大学 | One plant of root telogenesis film bacterium RS2 with luxuriant and rich with fragrance degradation function and its application |
| CN105112345B (en) * | 2015-09-30 | 2018-09-28 | 广东省微生物研究所 | A kind of sphingolipid bacterium (Sphingobium sp.) IBY and its application in absorption degradation hydrophobic organic compound |
| CN109439573B (en) * | 2018-10-30 | 2021-06-01 | 南京农业大学 | Bacterial strain with specific conversion function on S-napropamide, amidohydrolase, coding gene and application thereof |
| CN110734882B (en) * | 2019-11-25 | 2022-07-05 | 上海交通大学 | Phenanthrene efficient degradation strain and application thereof in environmental remediation |
| CN112852660A (en) * | 2021-01-05 | 2021-05-28 | 西南科技大学 | Microbial agent for repairing coking soil and application thereof |
| CN113695370B (en) * | 2021-08-30 | 2022-06-07 | 河北科技大学 | Natural eutectic ionic liquid and preparation method and application thereof |
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