CN115006309B

CN115006309B - Microcirculation-improving whitening composition, microcirculation-improving whitening essence, and preparation methods and applications thereof

Info

Publication number: CN115006309B
Application number: CN202210759742.7A
Authority: CN
Inventors: 姜姗姗; 李燕; 韩婷婷; 杨素珍; 袁春颖; 刘三岭; 白天明; 陈玉荣; 徐佩佩
Original assignee: Shandong Furida Biological Co ltd
Current assignee: Shandong Furida Biological Co ltd
Priority date: 2022-06-30
Filing date: 2022-06-30
Publication date: 2023-09-22
Anticipated expiration: 2042-06-30
Also published as: CN115006309A

Abstract

The application belongs to the technical field of cosmetics, and particularly relates to a microcirculation-improving whitening composition, a microcirculation-improving whitening essence, a preparation method and application thereof. The microcirculation-improving whitening composition comprises the following components in parts by mass: 1 to 10 parts of herba lycopi extract, 0.5 to 8 parts of pummelo peel extract and 0.1 to 12 parts of comfrey extract. The application utilizes the synergistic effect among the three extracts to ensure that the microcirculation improving whitening composition and the essence containing the microcirculation improving whitening composition obviously inhibit the over-expression of AQP-1, thereby inhibiting the increase of vascular permeability, improving the microcirculation and realizing the effects of improving red blood streak, edema, eye puffiness and black eye; meanwhile, the skin whitening cream can inhibit the activity of tyrosinase, has an inhibiting effect on melanin generation, maintains the physiological activity of epidermal cells and promotes the metabolism of cells, has the effect of whitening skin, and has good practical application value.

Description

Microcirculation-improving whitening composition, microcirculation-improving whitening essence, and preparation methods and applications thereof

Technical Field

The application belongs to the technical field of cosmetics, and particularly relates to a microcirculation-improving whitening composition, a microcirculation-improving whitening essence, a preparation method and application thereof.

Background

The disclosure of this background section is only intended to increase the understanding of the general background of the application and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art.

The theory of Su Wen and Mai Yao essence proposes: the five colors of the feverish sensation in the chest and the heart are also known as the morbid state of qi. The red skin is wrapped with vermilion, not want to be like ocher; white is like goose feather, and salt is not; the green color is like the gloss of the pale wall, and the blue color is not desired; yellow is like Luo Guo Realgar, but not yellow; black is like heavy paint color, and is not like pale. The desire refers to any of five colors, and indicates that the five zang organs are smooth in essence and qi and blood, and the illness is mild and the prognosis is poor when the five colors are bright and moist and have no obvious effect; no matter what the five colors are, if the colors are dry and dark, the symptoms are bad and reverse. The complexion is divided into normal color and disease color, and the normal color can be divided into main color and guest color. The dominant color is basic skin color and complexion which the human life does not change. On this basis, some people can have a slight white, darker, reddish difference. Besides the main color, the complexion is also a guest color, namely the complexion changes caused by climate, diet, emotion changes, sports, working environment, age and the like, such as reddening of the face after sports, pale complexion in cold winter, darkening of the complexion after long-term outdoor work, aging, and the like. The common characteristic of the healthy faces of Chinese is that the red and yellow are hidden, clear and moist.

In modern medicine, the complexion is found to be closely related to blood microcirculation. The blood microcirculation is simply the blood circulation of capillary network, and can promote blood and tissue cells to carry out nutrition metabolism and discharge toxins. Such symptoms as eye blushing, skin color greying, darkening of stains, etc., all indicate problems with microcirculation. The lymphatic circulation may be referred to as a waste disposal plant for the body, and if it is slow, it is prone to build up of bodily waste and toxins. When the lymphatic circulation becomes slow, the most obvious change is manifested in that the eye bags become obvious, the skin color becomes blue, and even a series of problems such as acne, edema, constipation and the like can occur.

The inventor finds that the action effect of the whitening products on the market is single and the action time is not durable, so that the problem that the skin is dark and irrelevant cannot be fundamentally solved, and the reason is that the effect is not improved. Therefore, for the improvement of the complexion gas color, the skin care product capable of fundamentally solving the problem is required to be simultaneously compatible with natural and non-irritating, but the components in the natural and non-irritating skin care product can not all show the effects of the components, and the effects of the components can not be improved after being compounded and added into the product, so that the expected effect of improving the complexion gas color can not be achieved, and the effects can be inhibited mutually, so that the effect is poor.

Disclosure of Invention

Aiming at the defects existing in the prior art, the application aims to provide a microcirculation-improving whitening composition, a microcirculation-improving whitening essence, a preparation method and application thereof. Experiments prove that the composition and the microcirculation improving and whitening essence provided by the application inhibit the vascular permeability from increasing by obviously inhibiting the over-expression of AQP-1, so that the microcirculation is improved, the synergistic effect among components is improved, and the red blood streak, the edema, the eye bags and the black eye circles are effectively improved; meanwhile, the skin whitening cream can inhibit the activity of tyrosinase, inhibit melanin generation, maintain the physiological activity of epidermal cells and promote the metabolism of cells, and has the effect of whitening skin. Based on the above results, the present application has been completed.

In order to achieve the technical purpose, the technical scheme provided by the application is as follows:

in a first aspect of the application, there is provided a composition for improving microcirculation and whitening, comprising the following components in parts by mass: 1 to 10 parts of herba lycopi extract, 0.5 to 8 parts of pummelo peel extract and 0.1 to 12 parts of comfrey extract.

In a second aspect, the application provides an application of the microcirculation-improving whitening composition in preparing microcirculation-improving whitening essence. Proved by researches, the herba lycopi extract, the pummelo peel extract and the comfrey extract have obvious synergistic effect, so that the herba lycopi extract, the pummelo peel extract and the comfrey extract can be used for whitening and protecting skin; furthermore, the essence can be prepared by adding other effective active ingredients, so that the whitening and skin care effects of the essence are further improved, and the use is more convenient.

Accordingly, in a third aspect of the present application, there is provided an improved microcirculatory whitening essence comprising the above-described improved microcirculatory whitening composition.

The microcirculation-improving whitening essence comprises the following components in percentage by mass: 0.1 to 1 percent of thickening agent, 0.01 to 0.05 percent of EDTA-2Na, 0.1 to 1 percent of preservative, 4 to 8 percent of polyalcohol, 0.5 to 5 percent of humectant, 0.1 to 1 percent of natural grease, 0.01 to 1 percent of lecithin, 0.5 to 3 percent of panthenol, 1.5 to 8 percent of herba lycopi extract, 1 to 7 percent of pummelo peel extract, 0.5 to 10 percent of comfrey extract and the balance of water.

In a fourth aspect of the present application, a method for preparing the improved microcirculation whitening essence is provided, comprising the following steps:

s1, stirring and heating water, dipropylene glycol, sphingosine fungus glue, EDTA-2Na, 1, 2-hexanediol and sodium hyaluronate, and then keeping warm and continuing stirring until the materials are completely and uniformly dissolved to obtain liquid I;

s2, stirring and heating the white pond flower seed oil to obtain liquid II;

s3, maintaining the temperature of the water phase at a high temperature state, starting stirring, pouring the oil phase into the water phase solution, homogenizing after mixing, and stirring and cooling to obtain liquid III;

s4, continuing stirring, and adding the p-hydroxyacetophenone into the liquid III when the temperature is reduced to obtain a liquid IV;

and S5, maintaining the stirring rotation speed, and adding lecithin, panthenol, herba lycopi extract, exocarpium citri rubrum extract and comfrey extract into the liquid IV when the temperature is further reduced, so as to obtain the microcirculation improving and whitening essence.

The preparation process of the microcirculation improving whitening essence is optimized, so that the activity of the essence is ensured.

In a fifth aspect of the application, there is provided the use of the above composition and/or concentrate for the preparation of a whitening product for improving microcirculation.

The beneficial technical effects of one or more of the technical schemes are as follows:

(1) According to the technical scheme, the synergistic effect among the herba lycopi extract, the pummelo peel extract and the comfrey extract is utilized, so that the microcirculation improving and whitening composition and the cosmetic containing the microcirculation improving and whitening composition inhibit the over-expression of AQP-1, thereby inhibiting the increase of vascular permeability, improving microcirculation, realizing the effects of improving red blood streaks, removing edema, eye bags and black eyes, inhibiting the activity of tyrosinase, inhibiting the generation of melanin, maintaining the physiological activity of epidermal cells and promoting the metabolism of cells, and achieving the effect of whitening skin;

(2) The essence obtained by the technical scheme has the advantages of simple and feasible preparation method and low preparation cost, and therefore has good practical application value.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present application, and that other drawings may be obtained according to the provided drawings without inventive effort for a person skilled in the art.

FIG. 1 shows the expression level of AQP-1 after EC treatment with different substances by Western blot analysis in experimental example 1 of the present application;

FIG. 2 is a photograph showing blood flow of zebra fish blood vessels of different products in experimental example 2 of the present application;

FIG. 3 shows the improvement of blood flow velocity of internode blood vessels for different products in Experimental example 2 of the present application;

FIG. 4 shows the improved microcirculation efficacy of the different products of Experimental example 2;

FIG. 5 shows the inhibition of tyrosinase activity by different products of example 3 of the present application;

fig. 6 shows the change of uv color change of the subject in experimental example 5 of the present application after 28 days of using the improved microcirculation whitening essence.

Detailed Description

It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.

As described above, the whitening products on the market at present have single action effect and long action time, and cannot fundamentally solve the problem of skin darkness and independence, because the effect is not improved. Therefore, for the improvement of the complexion gas color, the skin care product capable of fundamentally solving the problem is required to be simultaneously compatible with natural and non-irritating, but the components in the natural and non-irritating skin care product can not all show the effects of the components, and the effects of the components can not be improved after being compounded and added into the product, so that the expected effect of improving the complexion gas color can not be achieved, and the effects can be inhibited mutually, so that the effect is poor.

In view of the above, in an exemplary embodiment of the present application, there is provided a composition for improving microcirculation and whitening, comprising the following components in parts by mass: 1 to 10 parts of herba lycopi extract, 0.5 to 8 parts of pummelo peel extract and 0.1 to 12 parts of comfrey extract.

In a further specific embodiment of the application, the composition comprises the following components in parts by mass: 1.5 to 8 parts of herba lycopi extract, 1 to 7 parts of pummelo peel extract and 0.5 to 10 parts of comfrey extract.

Wherein the herba Lycopi extract is obtained by concentrating and drying an extract prepared by heating and refluxing pretreated herba Lycopi with ethanol;

the pretreatment comprises the steps of drying, crushing and sieving (such as sieving with a 100-mesh sieve) the herba lycopi, and the pretreatment is performed, so that the effective components of the herba lycopi can be more effectively extracted by subsequent reflux extraction;

wherein the ethanol is high-concentration ethanol, preferably 90% ethanol; the mass ratio of the pretreated herba lycopi to the ethanol is 1:4-8, preferably 1:6;

the pummelo peel extract is obtained by concentrating and drying an extracting solution prepared by heating and refluxing pretreated pummelo peel with ethanol;

the pretreatment comprises the steps of drying, crushing and sieving (such as sieving with a 100-mesh sieve) the exocarpium Citri Grandis, and the effective components of the exocarpium Citri Grandis are more effectively extracted by carrying out pretreatment, thereby being beneficial to subsequent reflux extraction;

wherein the ethanol is high-concentration ethanol, preferably 70% ethanol; the mass ratio of the pretreated pummelo peel to the ethanol is 1:3-7, preferably 1:5;

the extract of the polymeric grass is obtained by concentrating and drying an extracting solution prepared by heating and refluxing the pretreated polymeric grass whole grass by ethanol;

the pretreatment comprises the steps of drying, crushing and sieving (such as sieving with a 100-mesh sieve) the comfrey, and the pretreatment is performed, so that the effective components of the comfrey can be more effectively extracted by subsequent reflux extraction;

wherein the ethanol is high-concentration ethanol, preferably 95% ethanol; the mass ratio of the pretreated comfrey to the ethanol is 1:6-10, preferably 1:8.

In still another embodiment of the present application, there is provided the use of the above-mentioned microcirculation-improving whitening composition for preparing a microcirculation-improving whitening essence. Proved by researches, the herba lycopi extract, the pummelo peel extract and the comfrey extract have obvious synergistic effect, so that the herba lycopi extract, the pummelo peel extract and the comfrey extract can be used for whitening and protecting skin; furthermore, the essence can be prepared by adding other effective active ingredients, so that the whitening and skin care effects of the essence are further improved, and the use is more convenient.

Accordingly, in still another embodiment of the present application, there is provided an improved microcirculatory whitening essence comprising the above improved microcirculatory whitening composition.

In another specific embodiment of the application, the microcirculation-improving whitening essence comprises the following components in percentage by mass: 0.1 to 1 percent of thickening agent, 0.01 to 0.05 percent of EDTA-2Na, 0.1 to 1 percent of preservative, 4 to 8 percent of polyalcohol, 0.5 to 5 percent of humectant, 0.1 to 1 percent of natural grease, 0.01 to 1 percent of lecithin, 0.5 to 3 percent of panthenol, 1.5 to 8 percent of herba lycopi extract, 1 to 7 percent of pummelo peel extract, 0.5 to 10 percent of comfrey extract and the balance of water.

In yet another embodiment of the present application, the thickener comprises sphingosine gel.

In yet another embodiment of the present application, the preservative comprises p-hydroxyacetophenone.

In still another embodiment of the present application, the polyhydric alcohol is any one or more of dipropylene glycol, glycerin, butylene glycol, 1, 2-hexanediol, and further preferably dipropylene glycol and 1, 2-hexanediol.

In still another embodiment of the present application, the humectant includes any one or more of sodium hyaluronate, urea, and betaine, and sodium hyaluronate and urea are further preferred.

In still another embodiment of the present application, the natural oil includes one or more of linseed oil and white pool seed oil, and more preferably white pool seed oil.

In another specific embodiment of the application, the microcirculation-improving whitening essence comprises the following components in percentage by mass: 0.2 to 0.8 percent of sphingosine bacterial gel, 0.03 to 0.07 percent of EDTA-2Na, 0.3 to 0.8 percent of p-hydroxyacetophenone, 3.5 to 6.5 percent of dipropylene glycol, 0.2 to 0.8 percent of 1, 2-hexanediol, 0.5 to 2.5 percent of urea, 0.08 to 0.3 percent of sodium hyaluronate, 0.25 to 0.8 percent of white pond flower seed oil, 0.02 to 0.8 percent of lecithin, 0.5 to 1.5 percent of panthenol, 3 to 6 percent of herba lycopi extract, 1.5 to 5.5 percent of exocarpium citri grandis extract, 1 to 7 percent of polymeric grass extract and the balance of water.

In still another embodiment of the present application, a preparation method of the above-mentioned improved microcirculation whitening essence is provided, comprising the following steps:

s1, stirring and heating water, dipropylene glycol, sphingosine fungus glue, EDTA-2Na, 1, 2-hexanediol, urea and sodium hyaluronate, and then keeping warm and continuously stirring until the components are completely and uniformly dissolved to obtain liquid I;

s2, stirring and heating the white pond flower seed oil to obtain liquid II;

s3, maintaining the temperature of the water phase (namely liquid I) at a high temperature state, starting stirring, pouring the oil phase (namely liquid II) into the water phase solution, homogenizing after mixing, and stirring and cooling to obtain liquid III;

It should be noted that, steps S1 and S2 are not sequentially divided.

In the step S1, the stirring and heating temperature is controlled to be 80-85 ℃;

in the step S2, the stirring and heating temperature is controlled to be 75-80 ℃;

in the step S3, the temperature of the water phase is kept at 8080-85 ℃, the stirring rotation speed is started and kept at 200-400 r/min, the oil phase is poured into the water phase solution, and after the mixing is finished, the homogenization is carried out for 5-10 min at 5000-8000 r/min, and then the stirring and the cooling are carried out;

in the step S4, stirring is continued, the stirring rotation speed is kept at 100-250 r/min, and p-hydroxyacetophenone is added into the liquid III when the temperature is reduced to 65-70 ℃;

in the step S5, the stirring rotation speed is kept at 100-250 r/min, and when the temperature is reduced to 40-48 ℃, lecithin, panthenol, herba lycopi extract, exocarpium citri rubrum extract and comfrey extract are added into the liquid IV.

In yet another embodiment of the present application, there is provided the use of the above-described composition and/or concentrate in the preparation of a whitening product for improving microcirculation.

In yet another embodiment of the present application, the product may be a cosmetic product, including in particular but not limited to facial washes, facial masks, creams, sunscreens, and the like.

Raw materials and functions:

herba Lycopi extract: herba Lycopi is firstly carried in Shennong BenCao Jing (Shennong's herbal book), and is recorded in the book of the past materia Medica, and mainly grows in wetlands such as swamp lands, watersides and ditches, and also has cultivated products distributed in places such as Heilongjiang, hebei, shangxi, henan, jiangsu, jiangxi and Sichuan. Has effects of promoting blood circulation, regulating menstruation, removing blood stasis, resolving carbuncle, inducing diuresis, and relieving swelling, and can be used for treating menoxenia, amenorrhea, dysmenorrhea, puerperal abdominal pain due to blood stasis, edema, etc. Modern researches have shown that the chemical components of herba Lycopi include structural compounds such as phenolic acids, flavonoids, terpenes and steroids, and especially contain effective components such as oleanolic acid, ursolic acid, caffeic acid, etc.

Pummelo peel extract: the medicinal phase of pummelo peel is found in south dynasty Song Shi, and has been known from ancient times as "southern ginseng" and "one-piece-value-one-gold". The Ming dynasty plum time delicacies (materia medica schema) are recorded in: "orange good product, its pulp has red and white components, and its actions of promoting qi circulation, resolving phlegm and relieving cough are more than those of its medicine …, its effect is Chen Yuliang". "Fuzhou orange red, treating phlegm like god," Zhengzhen Zhen Yuan "by Zhengzheng Sunzhen for fourteen years. Modern medicine proves that the main component of pummelo peel, namely "flavanone" and "naringin" (C ₂₇ H ₃₂ O ₁₄ ) The health care tea has extremely high content, contains effective components such as' peptide, and the like, has the effects of relieving cough and reducing sputum, is more noble, has remarkable functions of health care, beauty treatment and detoxification on cardiovascular and cerebrovascular of human body, and has unique health care and medical effects.

A comfrey extract: the comfrey is also called as Aiguo, feverfew, friendship, purple root, convalescence, exotic comfrey, siemens, radix Arnebiae, etc., and is also called as Russian fodder vegetable in production, called as Russian vegetable for short, which is grass of comfrey genus of Boraginaceae. The leaf and root of the vegetable are also vegetables with higher nutritive value and medicinal property, and can be used for treating incised wound, traumatic injury, ulcer, stomach ache, toothache, sore throat, diarrhea, etc. The comfrey contains allantoin, and can stimulate new cell growth, treat wound by external application, and promote wound healing. The medicinal rhizome has the functions of promoting blood circulation, cooling blood, clearing heat and detoxicating. In addition, the feed additive contains a large amount of allantoin and vitamin B12, so that enteritis of livestock and poultry can be prevented and treated.

Sodium hyaluronate: sodium hyaluronate, also known as sodium hyaluronate, is an acidic mucopolysaccharide naturally present in the cornea skin and capable of absorbing 1000 times the weight of the water to achieve the effects of keeping the skin moisture, preventing the moisture from losing through the epidermis, and repairing the barrier when the skin is damaged, so that the skin does not feel dry after being used, the luster is increased, the nutrition metabolism of the skin can be improved, the skin is soft, smooth, wrinkle-removing, elasticity-increasing, aging-preventing, and the skin is a good percutaneous absorption promoter while moisture is preserved.

Sphingosine fungus glue: the sphingosine fungus glue is polysaccharide from microbial fermentation sources, has smooth skin feel, is not astringent and sticky, and has thickening and suspending properties.

White pond flower seed oil: the white pond flower seed oil is extracted from white pond flower, is a powerful natural antioxidant, and comprises the following components: 97% long carbon chain fatty acids, 20 or more carbon chains, 3 of which are not found in other vegetable oils. Has good affinity with skin, is easy to be absorbed by skin, is used for skin care products, can promote skin to be tender and elastic, and has good skin moistening and caring effects.

Urea: the urea contains nitrogen which is beneficial to human body, can promote protein synthesis, is a natural humectant in healthy skin, can reduce skin moisture loss, enhance skin moisturizing ability, enhance skin barrier and improve the penetration ability of other components.

Panthenol: the panthenol has small molecules and good permeability, has good hygroscopicity as many water-based moisturizers such as hyaluronic acid, can help to grasp moisture in the air, and can promote the permeation of water molecules to the skin, so that the skin can achieve the purposes of lasting and deep moisturizing. In addition to moisturizing effects, panthenol also stimulates the epithelial cells of the skin, promoting wound healing and relieving skin inflammation.

Lecithin: lecithin is a basic component of Natural Moisturizing Factor (NMF) of skin, and can bind moisture in skin, prevent skin from dehydration and dryness, and thus ensure skin softness, smoothness and health. Meanwhile, the phospholipid can effectively repair the damaged sebum membrane on the surface of the skin and promote the absorption of the skin.

The application is further illustrated below with reference to examples. The application is further illustrated by means of the following examples, which are not intended to limit the application thereto. Based on the embodiments of the present application, any changes to the present application without making any creative changes to the present application fall within the protection scope of the present application. Meanwhile, in the examples of the present application, all the preparation materials are commercially available products well known to those skilled in the art unless otherwise specified. Wherein in each embodiment, the herba Lycopi extract is prepared by drying herba Lycopi, pulverizing, sieving with 100 mesh sieve, heating and refluxing with 90% ethanol of 6 times of herba Lycopi mass, concentrating, and drying; the herb extract is prepared by drying herb, crushing, sieving with 100 mesh sieve, heating and refluxing with 95% ethanol with the mass of 8 times of herb, concentrating and drying; the pummelo peel extract is prepared by drying pummelo peel, crushing, sieving with a 100-mesh sieve, heating and refluxing an extracting solution with 70% ethanol with the mass of 5 times of the pummelo peel, concentrating and drying.

Example 1

The microcirculation-improving whitening composition comprises the following components in parts by mass: 5 parts of herba lycopi extract, 4 parts of pummelo peel extract and 2 parts of comfrey extract.

The microcirculation-improving whitening essence comprises the following components in percentage by mass: 0.25% of sphingosine bacterial gum, 0.05% of EDTA-2Na, 0.6% of p-hydroxyacetophenone, 5% of dipropylene glycol, 0.4% of 1, 2-hexanediol, 2% of urea, 0.1% of sodium hyaluronate, 0.4% of white pond flower seed oil, 0.1% of lecithin, 1.2% of panthenol, 5% of herba lycopi extract, 4% of exocarpium citri rubrum extract, 2% of comfrey extract and the balance of water.

The preparation method of the essence for improving microcirculation and whitening comprises the following steps:

(1) Stirring and heating water, dipropylene glycol, sphingosine fungus glue, EDTA-2Na, 1, 2-hexanediol, urea and sodium hyaluronate to 85 ℃, and keeping the temperature and continuing stirring until the components are completely and uniformly dissolved to obtain liquid 1;

(2) Stirring and heating the white pond flower seed oil to 75 ℃ to obtain liquid 2;

(3) Maintaining the temperature of the water phase at 885 ℃, starting stirring at 400r/min, pouring the oil phase into the water phase solution, homogenizing for 8min at 7000r/min after mixing, and stirring and cooling to obtain liquid 3.

(4) Maintaining the stirring speed at 220r/min, and adding p-hydroxyacetophenone into liquid 3 to obtain liquid 4 when the temperature is reduced to 65deg.C.

(5) Maintaining stirring speed at 220r/min, and adding lecithin, panthenol, herba Lycopi extract, exocarpium Citri Grandis extract and herba Agrimoniae extract into the liquid 4 when the temperature is reduced to 45deg.C to obtain the microcirculation improving whitening essence.

Example 2

The microcirculation-improving whitening composition comprises the following components in parts by mass: 3 parts of herba lycopi extract, 2 parts of pummelo peel extract and 6 parts of comfrey extract.

The microcirculation-improving whitening essence comprises the following components in percentage by mass: 0.3% of sphingosine bacterial gum, 0.03% of EDTA-2Na, 0.5% of p-hydroxyacetophenone, 4% of dipropylene glycol, 0.5% of 1, 2-hexanediol, 0.5% of urea, 0.15% of sodium hyaluronate, 0.6% of white pond flower seed oil, 0.3% of lecithin, 0.5% of panthenol, 3% of herba lycopi extract, 2% of exocarpium Citri Grandis extract, 6% of comfrey extract and the balance of water.

(1) Stirring and heating water, dipropylene glycol, sphingosine fungus glue, EDTA-2Na, 1, 2-hexanediol, urea and sodium hyaluronate to 80 ℃, and keeping the temperature and continuing stirring until the components are completely and uniformly dissolved to obtain liquid 1;

(2) Stirring and heating the white pond flower seed oil to 78 ℃ to obtain liquid 2;

(3) Maintaining the temperature of the water phase at 80deg.C, starting stirring at 260r/min, pouring the oil phase into the water phase solution, homogenizing at 5000r/min for 10min after mixing, and stirring and cooling to obtain liquid 3.

(4) Maintaining the stirring speed at 150r/min, and adding p-hydroxyacetophenone into liquid 3 to obtain liquid 4 when the temperature is reduced to 70deg.C.

(5) Maintaining stirring speed at 150r/min, and adding lecithin, panthenol, herba Lycopi extract, exocarpium Citri Grandis extract and herba Agrimoniae extract into the liquid 4 when the temperature is reduced to 40deg.C to obtain the microcirculation improving whitening essence.

Example 3

The microcirculation-improving whitening composition comprises the following components in parts by mass: 2 parts of herba lycopi extract, 6 parts of pummelo peel extract and 8 parts of comfrey extract.

The microcirculation-improving whitening essence comprises the following components in percentage by mass: 0.2% of sphingosine bacterial gum, 0.07% of EDTA-2Na, 0.3% of p-hydroxyacetophenone, 6.5% of dipropylene glycol, 0.8% of 1, 2-hexanediol, 2.5% of urea, 0.11% of sodium hyaluronate, 0.55% of white pond flower seed oil, 0.16% of lecithin, 0.6% of panthenol, 2% of herba lycopi extract, 6% of exocarpium Citri Grandis extract, 8% of comfrey extract and the balance of water.

The preparation method of the essence for improving microcirculation of the embodiment comprises the following steps:

(1) Stirring and heating water, dipropylene glycol, sphingosine fungus glue, EDTA-2Na, 1, 2-hexanediol, urea and sodium hyaluronate to 82 ℃, and keeping the temperature and continuing stirring until the components are completely and uniformly dissolved to obtain liquid 1;

(2) Stirring and heating the white pond flower seed oil to 72 ℃ to obtain liquid 2;

(3) Maintaining the temperature of the water phase at 82 ℃, starting stirring at 260r/min, pouring the oil phase into the water phase solution, homogenizing at 8000r/min after mixing, stirring and cooling to obtain liquid 3.

(4) Maintaining the stirring speed at 160r/min, and adding p-hydroxyacetophenone into liquid 3 to obtain liquid 4 when the temperature is reduced to 68 ℃.

(5) Maintaining stirring rotation speed at 160r/min, and adding lecithin, panthenol, herba Lycopi extract, exocarpium Citri Grandis extract and herba Agrimoniae extract into the liquid 4 when the temperature is reduced to 46 deg.C to obtain the microcirculation improving whitening essence.

Comparative example 1

The microcirculation improving whitening composition or the microcirculation improving whitening essence of this comparative example is different from that of example 1 in that: the microcirculation improving whitening composition or the microcirculation improving whitening essence of the comparative example does not contain herba lycopi extract and exocarpium citri grandis extract.

Comparative example 2

The microcirculation improving whitening composition or the microcirculation improving whitening essence of this comparative example is different from that of example 1 in that: the microcirculation-improving whitening or microcirculation-improving whitening essence of the comparative example does not contain herba lycopi extract and comfrey extract.

Comparative example 3

The microcirculation improving whitening composition or the microcirculation improving whitening essence of this comparative example is different from that of example 1 in that: the microcirculation-improving whitening or microcirculation-improving whitening essence of the comparative example does not contain pummelo peel extract and comfrey extract.

Comparative example 4

The microcirculation improving whitening composition or the microcirculation improving whitening essence of this comparative example is different from that of example 1 in that: the microcirculation-improving whitening or microcirculation-improving whitening essence of the comparative example does not contain herba lycopi extract.

Comparative example 5

The microcirculation improving whitening composition or the microcirculation improving whitening essence of this comparative example is different from that of example 1 in that: the microcirculation-improving whitening or microcirculation-improving whitening essence of the comparative example does not contain pummelo peel extract.

Comparative example 6

The microcirculation improving whitening composition or the microcirculation improving whitening essence of this comparative example is different from that of example 1 in that: the micro-circulation whitening improving or micro-circulation whitening essence of the comparative example does not contain the comfrey extract.

Comparative example 7

The microcirculation improving whitening composition or the microcirculation improving whitening essence of this comparative example is different from that of example 1 in that:

the herba lycopi extract is prepared by drying herba lycopi, crushing, sieving with a 100-mesh sieve, heating and refluxing an extracting solution with 75% ethanol of which the mass is 6 times that of the herba lycopi, concentrating and drying;

the herb extract is prepared by drying herb, crushing, sieving with 100 mesh sieve, heating and refluxing with 90% ethanol with the mass of 8 times of herb, concentrating and drying;

the pummelo peel extract is prepared by drying pummelo peel, crushing, sieving with a 100-mesh sieve, heating and refluxing an extract with 80% ethanol with the mass of 5 times of pummelo peel, concentrating and drying.

Comparative example 8

the herba lycopi extract is prepared by drying herba lycopi, crushing, sieving with a 100-mesh sieve, heating and refluxing an extracting solution obtained by heating and refluxing 95% ethanol with the mass of 6 times of herba lycopi, and concentrating and drying;

the herb extract is prepared by drying herb, crushing, sieving with a 100-mesh sieve, heating and refluxing an extracting solution with 75% ethanol of which the mass is 8 times that of the herb, concentrating and drying;

Experimental example 1 Effect of different test samples on AQP-1 expression

Endothelial cells were cultured in 12-well plates, each containing a polylysine-treated slide, and treated with HMGB1 or HMGB1 (100 ng/ml) and example 1, comparative example 2, comparative example 3, comparative example 4, comparative example 5, comparative example 6, comparative example 7, comparative example 8 (30 μg/ml) or controls, followed by fixation with 4% paraformaldehyde for 15 minutes, removal of the growing endothelial cells, and rinsing with PBS for 20 minutes at room temperature. After washing 3 times with PBS, cells were blocked with 5% BSA in PBS for 30min at room temperature and then incubated overnight with primary anti-AQP 1 antibody (1:200) at4 ℃. Endothelial cells were then washed twice with PBS (pH 7.4) and then exposed to FITC-labeled IgG secondary antibody (1:200) for 30min at room temperature in the dark. Nuclei were stained with DAPI for 1 min.

Western blot analysis determines AQP-1 expression: endothelial cells were washed with ice-cold PBS, then lysed with PMSF-containing RIPA lysis buffer (1:100) for 30min on ice, and then centrifuged at 12000g for 15min. Protein concentration was then determined using the diminutive acid (BCA) protein assay kit. The lysate was then heated in protein sample loading buffer at 95 ℃ for 10 minutes, and then all samples were kept at-20 ℃. Proteins (10 μl) were separated on 12.5% SDS-PAGE and transferred to PVDF membrane (on. After vigorous washing, membrane was incubated with HRP-conjugated secondary antibody (1:500) at room temperature for 1 hour at room temperature after washing three times with TBST, the bands were visualized using Omni ECL Femto-Light chemiluminescent kit and quantified using GE Amersham Imager 600 (USA), expression levels normalized to β -actin. Each of the above experiments were repeated at least 3 times.

AQP-1 (aquaporin-1) promotes the penetration of endothelial cells into water, and its overexpression induces increased vascular permeability. Thus, inhibition of AQP-1 overexpression can inhibit increased vascular permeability. As shown in the above figure 1, when the three extracts of herba Lycopi, exocarpium Citri Grandis and herba Agrimoniae are compounded, the expression of AQP-1 is significantly inhibited, so that the increase of vascular permeability is inhibited, and the inhibition effect is obviously superior to that of single use or two-by-two compounded use, and the compounded use of the three has obvious synergistic effect.

Experimental example 2 Effect of different test samples on blood flow velocity of blood vessels between zebra fish nodes

30 zebra fish were randomly selected in 6-well plates with 15 tails per well. Example 1, comparative example 2, comparative example 3, comparative example 4, comparative example 5, comparative example 6, and each well was fixed with 3mL of volume, and a normal control group was set. Then, each experimental group was incubated at 28℃for 24 hours in the absence of light. Finally, 10 zebra fish are randomly selected from each experimental group, blood flow videos of blood vessels among zebra fish nodes are recorded under a heart beat blood flow analysis system, and blood flow speeds (V) of the blood vessels among the zebra fish nodes are analyzed.

The sample improving microcirculation efficacy is calculated according to the following formula:

as can be seen from fig. 2-4, the effect of improving microcirculation when the three extracts are compounded is significantly better than that of single use or two-by-two compounding.

Experimental example 3 tyrosinase inhibition assay

Under the action of tyrosinase, L-tyrosine generates dopa, dopa generates dopaquinone under the further action of enzyme, and finally melanin is generated through a series of reactions. Wherein the dopaquinone has a maximum absorption peak at 475nm wavelength, and the absorbance is measured in unit time, so that the enzyme activity in the reaction system is catalyzed by the indirect reaction enzyme. I.e., an increase of 0.001 in absorbance at 475nm wavelength per minute of dopa-wakeup is defined as an enzyme activity unit. Accurately sucking the reaction solutions of the L-tyrosine, PBS and the sample in the T1, T2, T3 and T4 respectively according to the surface volumes by using a micropipette, respectively placing the reaction solutions in 4 PE pipes, uniformly mixing, keeping the temperature AT 37 ℃ for L0min, then respectively adding 1mL of tyrosinase in the T2 and T4, reacting the tyrosinase with the lOmin, and rapidly measuring the absorbance ATl, AT2, AT3 and AT4 AT 475nm by using an ultraviolet spectrophotometer. The tyrosinase inhibitory activity of the samples was calculated according to the following formula:

tyrosinase activity inhibition ratio = [1- (AT 4-AT 3)/(AT 2-AT 1) ] ×100%

Wherein, the absorbance of the reaction solution without the addition of the sample and without the addition of tyrosinase is measured AT 475nm

AT2 absorbance AT 475nm of the reaction solution without sample and tyrosinase

AT3 absorbance AT 475nm of the reaction solution with and without tyrosinase added

AT4 absorbance AT 475nm of the reaction solution containing the sample and tyrosinase

The results of the inhibition of tyrosinase activity in the above products are shown in FIG. 5.

Experimental example 4 evaluation of skin melanin and skin gloss of improving microcirculation whitening essence

The testing method comprises the following steps: the sample (example 1 improved microcirculatory whitening essence) was used twice daily, in the morning and evening, and was tried for 4 weeks. A proper amount of test sample was smeared on one side of the random pattern, and the test sample was not used as a control on the other side of the random pattern. The sunscreen product is applied to the whole face daily in the morning. The results were tested in the following 4 time periods, respectively: before the sample (D0), after 14 days of the sample (D14), and after 28 days of the sample (D28).

Detecting items:

1) The skin melanin determinator MX18 is used for detecting skin melanin;

2) The skin color analyzer CL200 is used to detect skin glossiness.

Test results:

1) Skin melanin

The subject was tested for target site melanin 28 days after the sample application (the measured value was reduced, indicating that the skin melanin content was less), the skin melanin value was significantly increased by 1.46% in the control area, while the area melanin was reduced by only 0.17% in the sample application. The prepared essence for improving microcirculation and whitening skin can effectively reduce the generation of skin melanin.

Results of detection of skin melanin content after 28 days in sample group and control group

Time	Before use	After 14 days of use	After 28 days of use
				Sample of	173.16	171.14	172.86
Control	174.44	175.21	176.99

Detection results of skin melanin content change rate of sample group and control group after 28 days

Time	After 14 days of use	After 28 days of use
			Sample of	-1.17％	-0.17％
Control	0.44％	1.46％

2) Skin gloss

The skin glossiness of the target part of the subject is tested 28 days after the sample is used (the measured value is increased, which means that the skin glossiness is better), the skin glossiness value of the control area is reduced by 2.42%, the skin glossiness of the sample area is increased by 1.99%, and the prepared microcirculation improving whitening essence can improve the skin glossiness.

Results of skin gloss measurements after 28 days in sample and control groups

Time	Before use	After 14 days of use	After 28 days of use
				Sample of	9.02	9.11	9.20
Control	9.1	8.91	8.88

Detection results of skin glossiness change rate of sample group and control group after 28 days

Time	After 14 days of use	After 28 days of use
			Sample of	0.99％	1.99％
Control	-2.09％	-2.42％

Experimental example 5Visia-CR facial ultraviolet stain analysis

A healthy female subject in China is selected with an age ranging from 30 to 40 years old. The facial photographs of the subjects were taken with a visual-CR before the subjects used the microcirculation whitening essence (D0), and the products were continuously used for 4 weeks (D28). And the larger the ultraviolet color spot area is analyzed by the image analysis of the ultraviolet color spot picture acquired and derived by the VISIA-CR, the more the skin potential pigment spots are. The subjects who had the improved microcirculatory whitening essence of example 1 applied the whitening essence after 28 days had significantly reduced and reduced uv spots, and the results are shown in figure 6.

It should be noted that the above examples are only for illustrating the technical solution of the present application and are not limiting thereof. Although the present application has been described in detail with reference to the examples given, those skilled in the art can make modifications and equivalents to the technical solutions of the present application as required, without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. The microcirculation-improving whitening essence is characterized by comprising the following components in percentage by mass: 0.1 to 1 percent of thickening agent, 0.01 to 0.05 percent of EDTA-2Na, 0.1 to 1 percent of preservative, 4 to 8 percent of polyalcohol, 0.5 to 5 percent of humectant, 0.1 to 1 percent of natural grease, 0.01 to 1 percent of lecithin, 0.5 to 3 percent of panthenol, 1.5 to 8 percent of herba lycopi extract, 1 to 7 percent of pummelo peel extract, 0.5 to 10 percent of comfrey extract and the balance of water;

the herba lycopi extract is obtained by concentrating and drying an extracting solution prepared by heating and refluxing pretreated herba lycopi with ethanol; the pretreatment comprises the steps of drying, crushing and sieving the herba lycopi; the mass ratio of the pretreated herba lycopi to the ethanol is 1:6; the ethanol is 90% ethanol;

the pummelo peel extract is obtained by concentrating and drying an extracting solution prepared by heating and refluxing pretreated pummelo peel with ethanol; the pretreatment comprises the steps of drying, crushing and sieving exocarpium Citri Grandis; the mass ratio of the pretreated pummelo peel to the ethanol is 1:5; the ethanol is 70% ethanol;

the extract of the polymeric grass is obtained by concentrating and drying an extracting solution prepared by heating and refluxing the pretreated polymeric grass whole grass by ethanol; the pretreatment comprises the steps of drying, crushing and sieving the comfrey; the mass ratio of the pretreated comfrey to the ethanol is 1:8; the ethanol is 95% ethanol.

2. The improved microcirculatory whitening essence according to claim 1, wherein the mesh size of each of herba lycopi, exocarpium Citri Grandis and comfrey is 100 mesh.

3. The improved microcirculatory whitening essence according to claim 1, wherein the thickener comprises sphingosine gel;

the preservative comprises p-hydroxyacetophenone;

the polyalcohol is any one or more of dipropylene glycol, glycerol, butanediol and 1, 2-hexanediol;

the humectant comprises one or more of sodium hyaluronate, urea and betaine;

the natural oil comprises one or more of linseed oil and white pool flower seed oil.

4. The improved microcirculatory whitening essence according to claim 3, wherein the polyalcohol is dipropylene glycol and 1, 2-hexanediol;

the humectant is sodium hyaluronate and urea;

the natural oil is white pool flower seed oil.

5. The improved microcirculation whitening essence as claimed in claim 1, which is characterized by comprising the following components in percentage by mass: 0.2 to 0.8 percent of sphingosine bacterial gel, 0.03 to 0.07 percent of EDTA-2Na, 0.3 to 0.8 percent of p-hydroxyacetophenone, 3.5 to 6.5 percent of dipropylene glycol, 0.2 to 0.8 percent of 1, 2-hexanediol, 0.5 to 2.5 percent of urea, 0.08 to 0.3 percent of sodium hyaluronate, 0.25 to 0.8 percent of white pond flower seed oil, 0.02 to 0.8 percent of lecithin, 0.5 to 1.5 percent of panthenol, 3 to 6 percent of herba lycopi extract, 1.5 to 5.5 percent of exocarpium citri grandis extract, 1 to 7 percent of polymeric grass extract and the balance of water.

6. The method for preparing the microcirculation-improving whitening essence as set forth in claim 5, which is characterized by comprising the following steps:

s2, stirring and heating the white pond flower seed oil to obtain liquid II;

7. The method of claim 6, wherein steps S1 and S2 are not sequentially separated;

in the step S3, the temperature of the water phase is kept at 80-85 ℃, the stirring rotation speed is started to be kept at 200-400 r/min, the oil phase is poured into the water phase solution, after the mixing is finished, the homogenization is carried out for 5-10 min at 5000-8000 r/min, and then the stirring and the cooling are carried out;

8. Use of the concentrate according to any one of claims 1 to 5 for the preparation of a whitening product for improving microcirculation; the product is a cosmetic.

9. The use according to claim 8, wherein the cosmetic product comprises a facial cleanser, a facial mask, a face cream and a sun cream.