CN115005197A

CN115005197A - Method for manufacturing fish bone anatomy specimen for integral two-side display

Info

Publication number: CN115005197A
Application number: CN202210794721.9A
Authority: CN
Inventors: 亢世华; 张程凯
Original assignee: Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Current assignee: Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Priority date: 2022-07-07
Filing date: 2022-07-07
Publication date: 2022-09-06
Anticipated expiration: 2042-07-07
Also published as: CN115005197B

Abstract

The invention provides a method for manufacturing an integral fish bone anatomy specimen for two-side display, which belongs to the field of specimen manufacturing. The present invention combines fish's anatomical skeleton specimen with conventional fish soaking specimen, and makes it possible to use one specimen in fish anatomy research, fish morphology research, scientific research, teaching use and viewing use.

Description

Method for manufacturing fish bone anatomy specimen for integral two-side display

Technical Field

The invention belongs to the field of specimen preparation, and particularly relates to a preparation method of an integral fish skeletal anatomy specimen for two-side display.

Background

In conventional science popularization and teaching activities, the types of common fish specimens include dried specimens, soaked specimens, bone specimens and the like. The fish soaking specimen refers to a specimen which is not dissected after the death of a fish body, keeps the external character characteristics (except the body color) before the death of the fish body from being damaged and is soaked and preserved in a specific preserving fluid; the skeleton specimen is prepared by removing all tissues outside the skeleton, degreasing, bleaching and adhering and fixing by using glue. The two specimens respectively carry different biological information, in the course teaching, the common immersed specimen provides real and intuitive external morphological materials, and the bone specimen has important significance for the evolution of vertebrate bones and the evolution research of life science. The fish skeleton anatomical specimen is mostly used for exhibition of science popularization in museums and specimen halls or education and teaching of related specialty of fish science in all universities and colleges. However, due to the high difficulty of the manufacturing technology and the long time consumption, only a few fish museums have corresponding collections until now, and most of the fish museums are simple bone specimens. Many bone specimens lose the properties of joints and cartilages in the process of removing soft tissues, and the spatial positions of some parts are wrong due to improper operation when the bone specimens are attached and connected, so that the science popularization teaching value of the bone specimens is influenced. The skeleton specimen is usually dried, and the outer layer is brushed with resin or varnish for corrosion prevention, but the skeleton specimen is easy to shrink and deform and dry and crack after being contacted with air for a long time, and the skeleton specimen of small and medium-sized fishes is difficult to store for a long time. Moreover, the skeleton specimen only keeps the skeleton, so that the external morphological characteristics of the fish are completely lost, the exhibition of professional fish teaching is not facilitated, and for the popular science, the visitors are difficult to visually feel the association between the fish body and the skeleton of the fish body. Therefore, in most teaching scenes and scientific research processes, research can be performed only on one fish specimen, and the obtained data and conclusion are limited.

Disclosure of Invention

The invention aims to provide a method for manufacturing fish bone anatomy specimens for integral two-side display, which has the characteristics and advantages of both immersion specimens and bone specimens and can respectively display the external morphological characteristics and the internal bone structure of a fish body on the left side and the right side of the fish body.

The invention is realized by adopting the following technical scheme:

a method for manufacturing fish bone anatomy specimens for two-side display comprises the steps of reserving the original appearance of a treated fish immersed specimen on one side, carrying out bone dyeing and anatomy treatment on the other side to obtain a dyed bone specimen, embedding the dyed bone specimen on a middle partition plate by using high-strength transparent liquid silica gel along the longitudinal section of the central axis of the specimen, and respectively storing two sides of the specimen in different storage solutions, so that the two sides of the middle partition plate can respectively display the external morphological characteristics and the internal bone structure of a fish body.

Further, the used preserving fluid for the immersed specimen is 70-75% alcohol; the bone specimen preserving fluid comprises (by mass) liquid paraffin containing ginger oil 0.05-0.1%, coriander seed oil 0.05-0.1%, zedoary turmeric oil 0.1-0.2% and cedar oil 0.1-0.2%, and has neutral pH value.

Further, the method comprises the steps of preparing a fish body soaking specimen, dyeing bones, dissecting one side, preparing a middle partition plate, inlaying the specimen, preparing a preserving fluid and preserving;

the preparation of the fish body infusion specimen is to treat the whole fish body according to the preparation method of the infusion specimen;

the bone staining is to stain the bone on one side of the specimen;

the unilateral dissection is to dissect one side of the dyed bone to make a bone specimen;

the middle partition board is manufactured by manufacturing the middle partition board with a fish-shaped hole in the middle, wherein the fish-shaped hole is larger than the outline of the specimen to be embedded;

the specimen embedding is to embed the specimen on the middle partition plate, and two sides of the middle partition plate are liquid-tight after embedding;

preparing and storing the preservation solution, wherein the preservation solution on the impregnated specimen side is 70% alcohol; the bone specimen preserving fluid comprises, by mass, liquid paraffin containing ginger oil 0.05-0.1%, coriander seed oil 0.05-0.1%, zedoary turmeric oil 0.1-0.2%, and cedar oil 0.1-0.2%, and has neutral pH value;

adding a soaked specimen preservation solution and a bone specimen preservation solution into two sides of the specimen display box respectively to serve as preservation solutions of a soaked specimen side and a bone specimen side; sealing the two sides of the specimen display box, and storing at 15-25 deg.C in a place without sunlight irradiation.

Further, the method also comprises the step of converting the prepared specimen into a preservation type specimen, cutting off the inlaid specimen along the outer contour, removing the silica gel without damaging the fish body, removing the silica gel remained on the specimen after soaking in petroleum ether, and converting into a soaking preservation type specimen for preservation after being washed clean by clear water.

Further, the high-strength transparent liquid silica gel is 10-degree transparent liquid silica gel.

Further, for fishes above 1KG, fumed silica is added into 10-degree transparent liquid silica gel, colorless silica powder and alumina powder are added into the 10-degree transparent liquid silica gel along with the increase of the weight of the fish body, the adding amounts of the colorless silica powder and the alumina powder are gradually increased along with the increase of the weight of the fish body so as to increase the hardness of the silica gel, and the adding mass ratios of the colorless silica powder and the alumina powder are 0.5% -2% and 1% -5%, respectively.

Further, the bone dyeing uses the inside dyeing apparatus of unilateral, the device includes outer box body and interior box body, outer box body is a box body, interior box body comprises two lateral walls and the bottom plate of first baffle, second baffle, outer box body, and first baffle and second baffle are fixed in the inside of outer box body, and the space between first baffle and second baffle and the outer box body forms the dye liquor pond, and interior box body bottom has the space with outer box body bottom, and the inside dye liquor pond of constituteing of interior box body outside and outer box body communicates each other, the bottom plate of interior box body is equipped with a rubber material sample quiet platform, and the sample is stood and is run through metal capillary from top to bottom, and metal capillary can be according to the shape transform of waiting to dye the sample and become the sample shape, and metal capillary can reciprocate, and metal capillary and dye pond intercommunication.

Further, the method also comprises the step of taking a bone picture by using X-ray or CT, and restoring the original position of the bone by referring to the bone picture when the bone specimen is dissected or cemented.

Compared with the prior art, the invention has the beneficial effects that:

1) the invention combines the fish skeletal anatomy specimen with the conventional fish immersed specimen, so that one side of the same specimen shows the morphological characteristics of the conventional immersed specimen, and the other side shows the skeletal anatomy characteristic, and the invention can be simultaneously applied to fish anatomy research and fish morphology research, and can also be simultaneously applied to scientific research, teaching and ornamental purposes.

2) The invention dyes the skeleton on one side of the prepared specimen into red, and compared with the common milky white skeleton specimen, the dyeing ensures that the shape and the position of the bone and the connection between the bone and the bone are easier to observe, the characteristics are more obvious, and the appearance is more beautiful visually.

3) The specimen prepared by the method can be used for long-time display and preservation, the left side and the right side adopt different preservation solutions for respective preservation, the soaked specimen preservation solution can well preserve the morphological characteristics of the soaked specimen, but the bone surface is easy to dye and pigment out, the bone preservation solution can well preserve the bone shape and the dyed color, but the long-time soaking can cause certain damage or influence on soft tissues, the two sides of the middle clapboard of the middle fish-shaped hole are isolated, the preservation of the two sides can not be communicated, and therefore the specimen on the two sides of the whole body can be completely preserved.

4) The formula of the bone preservation solution for the specimen prepared by the method has strong stability, no volatility, no toxicity, no flammability and no chemical reaction with a contact substance, and compared with the specimen preservation solution prepared by conventional solvents such as water, alcohol, glycerol and the like, alizarin red s is insoluble in the bone preservation solution, can not cause the phenomenon that bones are decolorized or pigments are separated out into the preservation solution, has good color preservation effect, and the adopted preservative system is safe and effective, and can effectively kill and inhibit fungi and bacteria, thereby preserving the bone specimen for a long time.

5) The invention considers various aspects when screening the preservation solution at two sides, ensures that the preservation solution at two sides can not cause volume change (shrinkage or expansion) of the specimen while meeting different preservation requirements at two sides, and ensures that the density and the hydraulic pressure of the liquid at two sides are generally the same (the density of the preservation solution for soaking the specimen is about 0.86 g/cm) ³ The density of the bone preservation solution is 0.85-0.87g/cm ³ ) And deformation and damage of the sample mosaic part caused by hydraulic pressure difference can not be caused.

6) The high-strength transparent liquid silica gel for embedding the specimen meets the following conditions: (1) the sealing performance is good, and the different specimen preservation solutions on two sides are prevented from communicating; (2) the solidification speed is high (the specimen can not be exposed in the air for a long time, and the oxidation of the specimen is prevented); (3) the glue has certain strength, one side of the specimen is a soaked specimen, and the other side of the specimen is a skeleton specimen, so that the gravity of the two sides is unequal, and therefore, the glue for embedding has certain strength and can keep the specimen stable for a long time; (4) does not react with the preservation solution; (5) can be disassembled without damaging the fish body.

7) When the method is used for unilateral bone anatomy, a soft X-ray radiography system or an animal CT imaging system is adopted to take a picture of the bone as a reference, and a professional fish anatomy technical skill is adopted, so that the accuracy of the space structure of each part of the bone specimen is greatly ensured.

8) The reagent and the material adopted by the method of the invention have no toxicity, no bad smell, low price and lasting specimen preservation effect, and are suitable for exhibition and display.

9) The display type specimen prepared by the invention can be detached from the display box and converted into a preservation type specimen for other deep scientific researches.

Drawings

Fig. 1 is a diagram of an integral two-sided specimen of sebastes schlegeli; a is the side of the impregnated specimen, and b is the side of the bone specimen.

FIG. 2 is a schematic view of a single-side dyeing apparatus, in which: 1. the device comprises an outer box body, 2, a first partition plate, 3, a second partition plate, 4, a bottom plate, 5, a dye liquor pool, 6, a specimen standing table, 7 and a metal capillary tube.

Detailed Description

The technical solution of the present invention is further explained by the following examples, but the scope of the present invention is not limited in any way by the examples.

EXAMPLE 1 preparation of specimen preservation solution

The common specimen preserving fluid is 70% alcohol, which is prepared by mixing absolute ethyl alcohol and pure water according to the weight ratio of 7: 3 weight ratio. The immersed specimen preservation solution can well preserve morphological characteristics of the immersed specimen, but is easy to separate out bone surface dyeing pigment, so the immersed specimen preservation solution can only be used for preserving the immersed specimen side and cannot be used for preserving the bone specimen side. The preservation solution for the stained bone specimen side needs to meet the following requirements: (1) the coating is colorless and transparent, has strong stability, is not easy to burn and volatilize, and is not easy to generate physical and chemical property change; (2) the bone shape and the dyed color can be effectively preserved, the bone specimen can not be damaged, and the condition of pigment precipitation can not be caused; (3) the liquid density is approximately equal to 70% alcohol, and hydraulic pressure difference on the left side and the right side caused by overlarge density difference can not cause deformation of the inlaid specimen and the middle partition plate; (4) has better antiseptic effect, and has long-term aging (5) no toxicity, no volatility and no pungent smell. According to requirements, reagents such as water, formalin, alcohol, glycerol, diethylene glycol, benzene, chloroform, cyclohexane, liquid paraffin, acetic acid tincture, phenol, acetone and the like and various mixed preservation solutions are screened (see table 1 for details), and finally refined liquid paraffin is selected as a main component of the preservation solution, so that the preservation requirements of the 1 st, 2 nd, 3 th and 5 th items are met. The refined liquid paraffin can be used for manufacturing washing powder, synthetic detergent, synthetic petroleum protein, pesticide emulsifier, synergist and the like, is commonly used as emollient oil, oily excipient and solvent, is also used as a lubricant or an infiltration solution for blood gas analysis and pathological analysis in the medical field, but is fresh for manufacturing biological specimens. In order to achieve the antiseptic effect, oily substances are selected as the antiseptic according to the physicochemical properties of the liquid paraffin. According to the inspection of the literature and experiments, the formula of 0.05 percent of ginger oil, 0.05 percent of coriander seed oil, 0.1 percent of zedoary turmeric oil and 0.1 percent of cedar oil is finally determined to have good killing and inhibiting effects on fungi and bacteria, the integral physicochemical characteristics are not influenced, and the preservation solution has no obvious color change and no unpleasant odor.

The bone preservation solution can well preserve the bone shape and the dyed color, but the long-time infiltration can cause certain damage or influence on soft tissues, so the bone preservation solution cannot be used for impregnating a specimen side. Different preserving fluids must be used on two sides, and the density of the preserving fluids on the two sides has no big difference.

TABLE 1 Condition table of whether different reagents and solutions meet 5 requirements

	Requirement 1	Requirement 2	Requirement 3	Requirement 4	Requirement 5
						Water (W)	√	×	×	×	√
Formalin	√	×	×	√	×
						Alcohol	√	×	√	√	×
Glycerol	√	×	×	Can be added with antiseptic at later stage	√
						Diethylene glycol	√	×	×	Can be added with antiseptic at later stage	√
Benzene and its derivatives	×	√	√	Can be added with antiseptic at later stage	×
						Chloroform	×	√	×	√	×
Cyclohexane	×	×	×	Can be added with antiseptic at later stage	×
						Liquid paraffin	√	√	√	Can be added with antiseptic at later stage	√
Acetic acid tincture	×	×	×	Can be added with antiseptic at later stage	×
						Phenol and its preparation	×	×	×	√	×
Acetone (II)	×	×	×	Can be added with antiseptic at later stage	×

EXAMPLE 2 formulation of high-Strength clear liquid silica gel composition

When glue for inlaying the specimen is selected, more than thirty types of glue such as 705 silicone rubber, epoxy resin glue, 10-degree transparent liquid silicone rubber, silicone glue, transparent glass glue and the like are selected from the market for inlaying experiments. The glue selected needs to meet the following requirements: (1) the transparency is high, and the two preservation solutions do not react to generate the change of physicochemical properties; (2) the specimen is enough in mechanical strength and flexible, can be supported to be embedded on the middle partition plate, and cannot be easily damaged; (3) the adhesive can be well attached to the surface of a specimen, the specimen is not damaged, and the adhesive can be removed by using a certain mild organic solvent to disassemble the specimen; (4) has no toxicity and persistent volatile pungent odor. Experiments show that the epoxy resin adhesive has too strong adhesiveness and damages a specimen to a certain extent, 705 silicon rubber, silica gel adhesive and transparent glass adhesive can react with a preservation solution to cause dissolution, softening or color change turbidity, and only 10-degree transparent liquid silica gel meets the requirements. However, in the setting process, the mechanical strength was slightly insufficient when handling specimens of 1KG or more, and therefore, a control test was conducted by selecting additives which are added with ultrafine epoxy resin powder, titanium dioxide, alumina and colorless silica powder as additives for improving the mechanical strength, depending on the characteristics of the 10-degree transparent liquid silica gel. The first comparison component is a blank group, a group added with 0.5% of colorless silica powder, a group added with 1% of titanium dioxide, a group added with 2% of titanium dioxide, a group added with 1% of aluminum oxide, a group added with 2% of aluminum oxide, a group added with 0.5% of superfine epoxy resin powder and a group added with 1% of superfine epoxy resin powder. By comparison, the mechanical strength of several groups of liquid silica gels added with colorless silica micropowder and alumina is improved to different degrees, and the transparency and other characteristics are not influenced. Further experiments are carried out, the second control component comprises a group of adding 0.5% of colorless silica powder, a group of adding 1% of colorless silica powder, a group of adding 2% of colorless silica powder, a group of adding 1% of alumina, a group of adding 2% of alumina, a group of adding 5% of alumina, a group of adding 0.5% of colorless silica powder and 1% of alumina, a group of adding 1% of colorless silica powder and 2% of alumina, and a group of adding 2% of colorless silica powder and 5% of alumina, and the obtained results show that the mechanical strength of the liquid silica gel simultaneously added with the two substances is better than that of the liquid silica gel singly added with one substance, and the addition of 2% of colorless silica powder and 5% of alumina greatly improves the hardness of the liquid silica gel but obviously reduces the toughness, and the addition amount is the upper limit of the addition amount. Therefore, the sample is finally determined to be inlaid by using 10-degree transparent liquid silica gel, and the adding amounts of the colorless silica powder and the alumina powder are gradually increased according to requirements, wherein the adding mass ratios of the colorless silica powder and the alumina powder are respectively 0.5% -2% and 1% -5%.

Example 3

A method for manufacturing fish bone anatomy specimens for two-side display comprises the steps of reserving the original appearance of a treated fish immersed specimen on one side, carrying out bone dyeing and anatomy treatment on the other side to obtain a dyed bone specimen, embedding the dyed bone specimen on a middle partition plate by using high-strength transparent liquid silica gel along the longitudinal section of the central axis of the specimen, and respectively storing two sides of the specimen in different storage solutions, so that the two sides of the middle partition plate can respectively display the external morphological characteristics and the internal bone structure of a fish body. The impregnated specimen may be prepared from absolute ethyl alcohol or formaldehyde, and the specimen preservation solution may be the preservation solution selected in example 1, or other preservation solutions capable of achieving the same technical effects in the prior art.

Example 4

1) Preparing a fish body soaking specimen:

selecting fresh and well-tasted sebastes schlegeli hilgendorf of 35cm, cleaning, shaping, and stretching the fins, and then placing into absolute ethyl alcohol fixing liquid for fixing for 3-5 days. After the fixation is finished, the soaked specimen is soaked in 75% alcohol, and the liquid is changed for 2 to 3 times, each time for 5 to 7 days, until the soaked specimen is stable, no pigment precipitation or obvious change of soft tissues is generated, and the soaked specimen is stored in 70% alcohol preservation liquid.

2) Photographing bones:

and (3) placing the prepared soaked specimen in a soft X-ray photography system, and taking a picture of the bone, wherein the picture of the bone is required to clearly display the level, the position and the form of each part of the bone.

3) Bone staining:

alizarin red S powder 1g was dissolved in 5L 70% ethanol to prepare a bone staining solution, and the solution was allowed to stand for 24 hours. One side of the skin of the fish specimen having the bone photograph taken was removed with a scalpel, and the side was subjected to bone staining treatment for 4 days using a one-side internal staining apparatus.

The single-side internal dyeing device comprises an outer box body and an inner box body, as shown in figure 2, the device comprises an outer box body 1 and an inner box body, the outer box body 1 is a box-shaped body, the inner box body consists of a first clapboard 2, a second clapboard 3, two side walls of the outer box body 1 and a bottom plate 4, the first clapboard 2 and the second clapboard are fixed in the outer box body 1, a dye bath 5 is formed by the space between the first clapboard 2 and the second clapboard 3 and the outer box body 1, the bottom of the inner box body and the bottom of the outer box body 1 have a space of 3cm, the outer part of the inner box body is communicated with the dye bath 5 formed in the outer box body 1, the bottom plate 4 of the inner box body consists of a specimen static placing table 6, the specimen static placing table 6 is a rubber material area, a metal capillary tube 7 is penetrated up and down in the rubber material area, the metal capillary tube 7 can be transformed into a specimen shape according to-be dyed, and the metal capillary 7 can move up and down, and the metal capillary 7 is communicated with an external dyeing tank.

The metal capillary tubes 7 are inserted into the rubber material area, and the specific positions and the number of the metal capillary tubes 7 can be arranged according to the size and the shape of the specific fish specimen and the position of the required dyeing area. One side of a fish specimen to be dyed is placed on the capillary tube and slightly pressed down, so that the capillary tube is pricked into the tissue of the fish body, the inserting position and depth of the capillary tube are adjusted according to the required dyeing area, then the fish specimen dyeing liquid is poured into the dye liquid pool 5, and under the dual functions of the capillary action and the siphon action, the internal structure of the fish body can be dyed more uniformly and thoroughly.

When single-sided staining was performed: the fish is placed on the dyeing device, the capillary is inserted into the lower half part, and the opening (top end) of the capillary is close to but not more than the central axis of the fish body, so that the fish reaches the vicinity of the planes of the vertebra, the spinal cord spine and the vein spine. The upper surface of the fish is covered with the wet tissue, the moisture of the upper half part is kept, when bone staining solution is poured into the staining solution pool 5, the height difference between the liquid level of the staining solution and the opening of the capillary tube is adjusted according to the thickness of the muscle of the fish body, the thicker the muscle layer is, the larger the liquid level difference is, the hydraulic pressure formed by the generated liquid level difference enables the staining solution to upwards enter the fish body through the capillary tube and diffuse out, because the lower surface of the fish body is exposed in the air, the evaporation of the preservation solution and the moisture can drive the liquid in the fish body to move towards the lower surface of the fish body, and under the action of gravity, the staining solution can be diffused out from the opening of the capillary tube under the dual action, and the bone in the muscle of the lower half part of the fish body is stained by downward permeation. The outside of the fish body can not be dyed, and particularly the upper side surface of the fish body is not influenced by dyeing.

4) Unilateral dissection:

adopting a fish anatomy professional dissection means, and carrying out fish anatomy treatment on the side where the skin is removed under a stereoscopic dissection microscope by referring to the skeleton picture. The dissection process is formulated for different fishes, and the dissected parts are periorbital bones, jaw bones, a suspension-gill cap bones, tongue arches, gill arches, brains, shoulder belts, a waist belt, a medial axis skeleton-odd fin branch bones and a tail skeleton in sequence. The soft tissues of all the bones are removed, only the joints or the ligament connection between necessary bones is reserved, the disassembled parts are bonded and assembled back to the fish body by using universal glue according to the requirements, the bonding position is mainly the joint, and the position is corrected according to the bone picture, so that the accuracy of space conformation is ensured.

5) Manufacturing a middle partition plate:

placing the anatomical specimen on a laboratory table, bending a stainless steel piece with the thickness of 0.3mm and the width of 3cm along the edge of the fish body contour by a circle a little bit larger to form a general contour, and paying attention to the fact that the tip of the fin line does not abut against the stainless steel piece. The contour of the iron sheet is fixed in the center of a silica gel mold matched with the size of the fish anatomy specimen storage box, and oily vaseline is sprayed on the inner side of the mold and the surface of the iron sheet. Glue A and glue B of the epoxy resin quick-drying glue (both glues are purchased from the market) are mixed according to the ratio of 3: pouring the mixture into a beaker according to the weight ratio of 1, uniformly mixing, stirring until the mixture is colorless and transparent, defoaming at the temperature of 2-8 ℃, uniformly pouring the mixture into a mould after defoaming, setting the thickness according to the size of a fish body to be about 3-6mm, standing for one day, and demolding after thorough solidification to obtain the epoxy resin plate with a fish-shaped hole in the middle.

6) Specimen inlaying:

the specimen is embedded into a fish-shaped hole of the epoxy resin plate by using 10-degree transparent liquid silica gel, and is fixed along the longitudinal section of the central axis, and the gap between bones on the central axis is filled to prevent the intercommunication of the preservation solutions on the right two sides. And after the silica gel is dried, placing the epoxy resin plate inlaid with the fish anatomy specimen in a clamping groove of the fish anatomy specimen display box, and fixing and sealing the epoxy resin plate by using glass cement.

7) Preparing and storing a preservation solution:

the used preservation solution for the immersed specimen is 70% alcohol, and absolute ethyl alcohol and pure water are adopted according to the proportion of 7: 3 weight ratio. The bone preservation solution is prepared by adding ginger oil 0.5g, coriander seed oil 0.5g, zedoary turmeric oil 1g and cedar oil 1g into 997g of refined liquid paraffin, mixing, and dissolving completely, wherein the pH value is neutral.

The two sides of the display box are respectively added with the immersed specimen preservation solution and the bone preservation solution as the preservation solutions of the whole surface and the bone surface. Sealing the display box, and storing at 15-25 deg.C without sunlight. After 3 months, the liquid on both sides is still clear and transparent without change, and the color and the shape of the specimen are preserved as new. The middle embedded glue is stable and has no cracking deformation. As shown in fig. 1.

8) And (3) disassembling and restoring the specimen:

when the fish bone anatomy specimen needs to be converted into a preservation type specimen, the epoxy resin plate embedded with the fish anatomy specimen can be detached from the display box, the specimen is cut off along the outer contour by a scalpel, although the hardness and toughness of the high-strength transparent liquid silica gel are higher, the cleaning by the scalpel is easier, and most of the silica gel can be removed without damaging the fish body. The silica gel remaining on the specimen can be soaked in petroleum ether for 15 minutes and removed with forceps. The cleaned anatomical specimen can be converted into a soaking preservation type specimen for preservation after being washed clean by clear water.

Claims

1. The method is characterized in that one side of the treated fish immersed specimen is kept in the original appearance of the immersed specimen, the other side of the immersed specimen is subjected to bone dyeing and dissection treatment to prepare a dyed bone specimen, then high-strength transparent liquid silica gel is embedded on a middle partition plate along the longitudinal section of the central axis of the specimen, and two sides of the immersed specimen are respectively stored in different preservation solutions, so that the immersed specimen can respectively show the external morphological characteristics and the internal bone structure of a fish body on the left side and the right side of the middle partition plate.

2. The method for preparing the fish skeletal anatomy specimen for two-sided display according to claim 1, wherein the specimen-immersing preservation solution is 70-75% alcohol; the bone specimen preserving fluid comprises liquid paraffin containing ginger oil 0.05-0.1 wt%, coriander seed oil 0.05-0.1 wt%, zedoary turmeric oil 0.1-0.2 wt% and cedar oil 0.1-0.2 wt%, and has neutral pH value.

3. The method for preparing a fish skeletal anatomy specimen for both-side display according to claim 1, wherein the high-strength transparent liquid silica gel is a 10-degree transparent liquid silica gel.

4. The method for preparing a fish skeletal anatomy specimen for both-side display according to claim 1, wherein colorless fine silica powder and alumina powder are added to 10-degree transparent liquid silica gel for 1KG or more of fish, and the amounts of the colorless fine silica powder and the alumina powder are gradually increased with the increase in the weight of the fish body, and the mass ratios of the amounts of the colorless fine silica powder and the alumina powder added are 0.5% to 2% and 1% to 5%, respectively.

5. The method for preparing the fish skeletal anatomy specimen for integral two-sided display according to claim 1, wherein the method comprises the steps of preparing a fish immersed specimen, dyeing bones, dissecting one side, preparing a middle partition plate, inlaying the specimen, preparing and storing a preservation solution;

the fish body soaking specimen is prepared by processing the whole fish body according to the preparation method of the soaking specimen;

the bone staining is to stain the bone on one side of the specimen;

6. The method for preparing a fish skeletal anatomy specimen for both-side display according to claim 5, wherein the method further comprises a step of changing the prepared specimen to a preserved specimen, cutting off the embedded specimen along the outer contour, removing the silica gel without destroying the fish body, removing the silica gel remaining on the specimen after immersing in petroleum ether, washing clean, and then changing to an immersion preserved specimen for preservation.

7. The method for manufacturing a fish skeletal anatomy specimen for displaying on both sides according to claim 5, wherein the skeletal dyeing is performed by using a single-side internal dyeing device, the device comprises an outer box and an inner box, the outer box is a box, the inner box comprises a first separator, a second separator, two side walls of the outer box and a bottom plate, the first separator and the second separator are fixed inside the outer box, a dye bath is formed by the space between the first separator and the second separator, the outer box and the gap between the first separator and the second separator, the bottom of the inner box and the bottom of the outer box are provided with a space, the outside of the inner box and the dye bath formed inside the outer box are communicated with each other, the bottom plate of the inner box is provided with a rubber specimen standing table, a metal capillary tube penetrates through the specimen standing table, the metal capillary tube can be transformed into a specimen shape according to the shape of the specimen to be dyed, and the metal capillary tube can move up and down, the metal capillary is communicated with the dyeing tank.

8. The method for producing a fish skeletal anatomy specimen for both-side display according to claim 5, further comprising a step of taking a skeletal photograph using X-ray or CT.