CN115003810A - 用于con-稀有密码子的trem组合物及相关用途 - Google Patents

用于con-稀有密码子的trem组合物及相关用途 Download PDF

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Publication number
CN115003810A
CN115003810A CN202080077056.0A CN202080077056A CN115003810A CN 115003810 A CN115003810 A CN 115003810A CN 202080077056 A CN202080077056 A CN 202080077056A CN 115003810 A CN115003810 A CN 115003810A
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trem
con
fragment
seq
absent
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Chinese (zh)
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C.E.哈杰丁
D.A.贝里
T.阿纳斯塔西亚迪斯
N.B.阿费扬
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Flagship Pioneering Inc
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Flagship Pioneering Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Cosmetics (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202080077056.0A 2019-11-04 2020-11-04 用于con-稀有密码子的trem组合物及相关用途 Pending CN115003810A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962930361P 2019-11-04 2019-11-04
US62/930,361 2019-11-04
PCT/US2020/058948 WO2021092064A1 (en) 2019-11-04 2020-11-04 Trem compositions for con-rare codons and related uses

Publications (1)

Publication Number Publication Date
CN115003810A true CN115003810A (zh) 2022-09-02

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ID=73654885

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080077056.0A Pending CN115003810A (zh) 2019-11-04 2020-11-04 用于con-稀有密码子的trem组合物及相关用途

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Country Link
US (1) US20220364092A1 (https=)
EP (1) EP4055163A1 (https=)
JP (1) JP2023500116A (https=)
KR (1) KR20220128611A (https=)
CN (1) CN115003810A (https=)
AU (1) AU2020379762A1 (https=)
CA (1) CA3160097A1 (https=)
MX (1) MX2022005362A (https=)
WO (1) WO2021092064A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102930465B1 (ko) 2017-11-02 2026-02-25 더 위스타 인스티튜트 오브 아나토미 앤드 바이올로지 ACE-tRNA를 이용한 유전 재할당을 통한 정지 코돈의 구조 방법
WO2021243301A2 (en) * 2020-05-29 2021-12-02 Flagship Pioneering Innovations Vi, Llc. Trem compositions and methods relating thereto
KR20230029685A (ko) * 2020-05-29 2023-03-03 플래그쉽 파이어니어링 이노베이션스 브이아이, 엘엘씨 Trem 조성물 및 이에 관련된 방법

Citations (5)

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WO2007083094A1 (en) * 2006-01-18 2007-07-26 University Court Of The University Of Dundee Prevention/treatment of ichthyosis vulgaris, atopy and other disorders
US20080318884A1 (en) * 2005-03-25 2008-12-25 The Uab Research Foundation Methods for Altering Gene Expression and Methods of Treatment Utilizing Same
WO2010141851A1 (en) * 2009-06-05 2010-12-09 Salk Institute For Biological Studies Improving unnatural amino acid incorporation in eukaryotic cells
WO2012006551A2 (en) * 2010-07-08 2012-01-12 The Brigham And Women's Hospital, Inc. Neuroprotective molecules and methods of treating neurological disorders and inducing stress granules
CN104837506A (zh) * 2012-10-02 2015-08-12 瓦克星治疗有限责任公司 免疫调节性小细胞及使用方法

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US5801030A (en) 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
US6693086B1 (en) 1998-06-25 2004-02-17 National Jewish Medical And Research Center Systemic immune activation method using nucleic acid-lipid complexes
CA2930665A1 (en) 2013-11-18 2015-05-21 Rubius Therapeutics, Inc. Synthetic membrane-receiver complexes
MA39819A (fr) 2014-04-01 2017-02-08 Rubius Therapeutics Inc Méthodes et compositions d'immunomodulation
US20180135012A1 (en) 2015-05-13 2018-05-17 Rubius Therapeutics, Inc. Membrane-receiver complex therapeutics
SI3402491T1 (sl) 2016-01-11 2022-05-31 Rubius Therapeutics, Inc. Sestavki in postopki v zvezi z multimodalnimi terapevtskimi celičnimi sistemi za indikacije raka
MX2019000205A (es) 2016-07-07 2019-09-23 Rubius Therapeutics Inc Composiciones y metodos relacionados con sistemas celulares terapeuticos que expresan arn exogeno.
US20180153989A1 (en) 2016-12-02 2018-06-07 Rubius Therapeutics, Inc. Compositions and methods related to cell systems for penetrating solid tumors
CN110520522A (zh) 2017-02-17 2019-11-29 鲁比厄斯治疗法股份有限公司 功能化红系细胞
CA3062426A1 (en) 2017-05-08 2018-11-15 Flagship Pioneering Innovations V, Inc. Compositions for facilitating membrane fusion and uses thereof
WO2020069199A1 (en) * 2018-09-26 2020-04-02 Case Western Reserve University Methods and compositions for increasing protein expression and/or treating a haploinsufficiency disorder
CA3125567A1 (en) * 2019-01-18 2020-07-23 Flagship Pioneering, Inc. Trem compositions and uses thereof
WO2020243560A1 (en) * 2019-05-31 2020-12-03 Flagship Pioneering, Inc. Uses of trem compositions to modulate trna pools

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080318884A1 (en) * 2005-03-25 2008-12-25 The Uab Research Foundation Methods for Altering Gene Expression and Methods of Treatment Utilizing Same
WO2007083094A1 (en) * 2006-01-18 2007-07-26 University Court Of The University Of Dundee Prevention/treatment of ichthyosis vulgaris, atopy and other disorders
WO2010141851A1 (en) * 2009-06-05 2010-12-09 Salk Institute For Biological Studies Improving unnatural amino acid incorporation in eukaryotic cells
WO2012006551A2 (en) * 2010-07-08 2012-01-12 The Brigham And Women's Hospital, Inc. Neuroprotective molecules and methods of treating neurological disorders and inducing stress granules
CN104837506A (zh) * 2012-10-02 2015-08-12 瓦克星治疗有限责任公司 免疫调节性小细胞及使用方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
PARMLEY JOANNA等: "Clustering of Codons with Rare Cognate tRNAs in Human Genes Suggests an Extra Level of Expression Regulation", PLOS GENETICS, vol. 5, no. 7, 1 January 2009 (2009-01-01), XP055778002, DOI: 10.1371/journal.pgen.1000548 *
ZAHN等: "Overexpression of an mRNA dependent on rare codons inhibits protein synthesis and cell growth", JOURNAL OF BACTERIOLOGY, vol. 178, no. 10, 1 May 1996 (1996-05-01), pages 2926 - 2933, XP055059438, DOI: 10.1128/jb.178.10.2926-2933.1996 *
黄明;蒋寅;周飞燕;付灿;雷文瑾;: "改造稀有密码子对rhFGF8a原核表达的影响", 细胞与分子免疫学杂志, no. 06, 18 June 2008 (2008-06-18) *

Also Published As

Publication number Publication date
JP2023500116A (ja) 2023-01-04
KR20220128611A (ko) 2022-09-21
WO2021092064A1 (en) 2021-05-14
AU2020379762A1 (en) 2022-05-26
MX2022005362A (es) 2022-09-23
US20220364092A1 (en) 2022-11-17
CA3160097A1 (en) 2021-05-14
EP4055163A1 (en) 2022-09-14

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