CN114984300A - 一种强韧抗菌水凝胶敷料及其制备方法 - Google Patents
一种强韧抗菌水凝胶敷料及其制备方法 Download PDFInfo
- Publication number
- CN114984300A CN114984300A CN202210600570.9A CN202210600570A CN114984300A CN 114984300 A CN114984300 A CN 114984300A CN 202210600570 A CN202210600570 A CN 202210600570A CN 114984300 A CN114984300 A CN 114984300A
- Authority
- CN
- China
- Prior art keywords
- tough
- hydrogel dressing
- antibacterial
- hydrogel
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0014—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0019—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
- A61L26/0033—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
- A61L26/0038—Gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/21—Acids
- A61L2300/214—Amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2477/00—Characterised by the use of polyamides obtained by reactions forming a carboxylic amide link in the main chain; Derivatives of such polymers
- C08J2477/04—Polyamides derived from alpha-amino carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/04—Oxygen-containing compounds
- C08K5/05—Alcohols; Metal alcoholates
- C08K5/053—Polyhydroxylic alcohols
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Materials For Medical Uses (AREA)
Abstract
本发明涉及一种强韧抗菌水凝胶敷料及其制备方法。该水凝胶敷料是基于聚乙烯醇、明胶、胶原蛋白等成胶骨架的氢键作用成胶,成胶前混入超支化聚赖氨酸,成胶后通过在离子溶液中浸泡增强力学性能。经由氢键作用物理成胶可避免化学交联方法中交联剂残留导致的毒性问题,并且通过霍夫迈斯特效应盐析增强克服了物理交联方法得到的水凝胶力学性能差的缺点。本发明制备的水凝胶敷料具有良好的拉伸、压缩性能和抗菌性能。本发明提供的水凝胶敷料适用于各类皮肤伤口,且制备方法简便、可重复性高、成本低,有望在抗菌皮肤敷料领域获得广泛应用。
Description
技术领域
本发明涉及生物医用高分子材料技术领域,特别涉及一种强韧抗菌水凝胶敷料及其制备方法。
背景技术
皮肤损伤是人体健康的严重威胁,会导致体内水分、热量流失和降低抵御外界病原体侵入的能力。伤口愈合包括止血、炎症、增殖和重塑四个过程。根据患者不同的损伤程度、年龄和健康状况以及异物、感染等外界因素,整个愈合过程将持续数天至数年不等。皮肤损伤多发,皮肤伤口的愈合是临床上的常见问题,每年用于治疗伤口,特别是慢性伤口的花费巨大,这为患者以及医疗系统带来了巨大的经济压力。为降低伤口护理的成本,并解决伤口愈合过程中存在的渗出液大量分泌、易感染、易产生疤痕等问题,继续开发功能性伤口敷料。随着伤口愈合理论的逐渐清晰,伤口敷料的形式也经历了很大的变迁。传统的伤口敷料,如纱布、绷带、棉垫等,为干性敷料,只能为伤口提供物理保护,对伤口愈合和预防感染的作用有限,且取下敷料时对伤口的粘附会造成继发性损伤;而现代敷料,如泡沫、水胶体、水凝胶等,基于潮湿环境的愈合理论,与传统敷料相比具有清创、保湿、防感染、抑制瘢痕化等优点,这其中水凝胶由于具有良好的吸渗性、保湿性、非粘连性、生物相容性以及可作载体等特点而受到广泛关注。
然而水凝胶敷料也具有其局限性。水凝胶是亲水的三维大分子网络,通过聚合物链之间的交联得到,交联方式可以分为物理交联和化学交联。化学交联需要引入交联剂,部分情况下还需要有机溶剂,而这些交联剂、有机溶剂无法从体系中彻底除去,残留物容易导致毒性问题。物理交联手段主要有氢键、范德华力、主客体作用、静电作用和循环冻融等。虽然物理交联避免了交联剂和有机溶剂的残留,但得到的水凝胶通常力学性能较差,不能满足皮肤伤口的日常活动需要,又或者制备过程复杂、能耗高,例如循环冻融。如何在避免交联剂和有机溶剂的残留的同时,保证水凝胶具有良好的力学性能,且制备方法简便、低廉,是水凝胶敷料领域面临的一大难题。
另一方面,水凝胶敷料的保湿性固然可以为伤口提供一个湿润的、有利于愈合的环境,却也为细菌的滋生提供了一个绝佳的场所。目前市面上大部分水凝胶敷料对于细菌感染无能为力,针对这一问题通用的改进方法是加入抗菌剂赋予水凝胶敷料抗菌能力。抗菌剂根据来源不同可分为天然抗菌剂、无机抗菌剂和有机抗菌剂,典型代表分别为壳聚糖、银离子和抗生素。壳聚糖来源广泛、绿色安全,但有效抗菌基团氨基数量较少导致其抗菌能力较弱;银离子有效作用时间长、无耐药性,但银离子多以纳米形式添加到水凝胶中,稳定性差,容易团聚,且对革兰氏阳性菌杀灭效果较差;抗生素是一类常见且有效的抗菌剂,然而其与细菌表面蛋白的单靶点结合作用机理容易导致细菌产生耐药性。因此研究人员迫切希望能够寻找到一种安全、高效、广谱抗菌、性质稳定、易获得、成本低、无毒、不易诱导耐药性的新型抗菌水凝胶敷料,一种思路是寻找富含氨基的抗菌剂,且该抗菌剂应具备能便捷混入水凝胶中并在应用时快速释放的能力。
发明内容
针对上述问题,本发明的目的是提供一种强韧抗菌水凝胶敷料及其制备方法。该水凝胶敷料在物理交联的基础上通过盐析作用增强力学性能,制备过程简单易重复;通过添加新型高效抗菌剂超支化聚赖氨酸而具备优异的抗菌性能,以用于皮肤伤口的抗菌、促愈合。
为实现上述目的,本发明提供以下的技术方案,一种强韧抗菌水凝胶敷料的制备方法,具体包括以下组分:
按重量计,每100份材料中含有5-20份主体材料、20-50份甘油、0.0025-0.3份超支化聚赖氨酸,其余为水。
一种强韧抗菌水凝胶敷料的制备方法,其制备工艺步骤如下:
将超支化聚赖氨酸与主体材料加入水与甘油的混合溶液中,搅拌加热使其充分溶解;溶解后的溶液静置成胶;将固体水凝胶切成所需形状,置于适当体积的重量分数为10-35%的离子溶液中浸泡8-48小时后取出,用水冲洗并擦干。
进一步地,所述工艺步骤中超支化聚赖氨酸分子量为3-7kDa。
进一步地,所述工艺步骤中主体材料为壳聚糖、胶原蛋白、海藻酸盐、透明质酸、聚乙二醇、明胶、聚氨酯、聚乳酸、聚乙烯吡咯烷酮、聚乙烯醇、聚丙烯酸、卡波姆中的一种或多种的组合。
进一步地,所述工艺步骤中加热条件为40-80℃下10-60分钟,静置条件为室温下30-60分钟或4℃下5-15分钟。
进一步地,所述工艺步骤中离子溶液的组成为:阴离子为酒石酸根、醋酸跟、铬酸根、柠檬酸根、硫酸根、硫酸氢根、碳酸根、碳酸氢根、磷酸二氢根、硫代硫酸根、氯离子中的一种,阳离子为铵根离子、锂离子、钾离子、钠离子、锰离子、钙离子、钡离子中的一种。
本发明相对于现有技术具有以下有益效果:
1.本发明提供的一种强韧抗菌水凝胶敷料,通过主体材料的氢键作用成胶,避免了因交联剂的加入而导致的毒性问题,并且仍能保持块状形态。
2.本发明提供的一种强韧抗菌水凝胶敷料,通过霍夫迈斯特效应盐析增强,使拉伸、压缩性能得到大幅提高,超越了已有敷料数十kPa的强度级别。
3.本发明提供的一种强韧抗菌水凝胶敷料,所含抗菌剂的浓度低于现有技术;超支化聚赖氨酸可以通过破坏细菌细胞膜、DNA以及提升细菌细胞内活性氧水平等机理起到高效广谱抑菌、杀菌作用。
4.本发明提供的一种强韧抗菌水凝胶敷料制备方法,工艺简便、操作简单、成本低、可重复性好,得到的水凝胶敷料适用于机械损伤性创面、热损伤性创面、溃疡性创面等各类皮肤创面。
附图说明
图1为本发明制备的水凝胶敷料的展示图;
图2为实施例2及对比例1中该水凝胶敷料的力学性能,其中,(a)、(b)分别为对比例1、实施例2中水凝胶敷料的压缩测试曲线,(c)、(d)分别为对比例1、实施例2中水凝胶敷料的拉伸测试曲线;
图3为实施例3中该水凝胶敷料的抑菌圈表观图;
图4为实施例4及对比例2中该水凝胶敷料的体外抗菌性能。
具体实施方式
以下结合实施例进一步说明本发明的技术方案,但这些实施例并不用于限制本发明。
实施例1:
第一步:称取25mg超支化聚赖氨酸(3kDa)和5g胶原蛋白加入至24.995mL超纯水和20mL甘油的混合溶液中,60℃下搅拌1小时使其溶解。
第二步:溶解后的溶液于室温下静置60分钟成胶。
第三步:将水凝胶切成片状,浸没于重量分数为15%的磷酸二氢钠溶液中24h,随后取出,用去离子水冲洗并擦干。
本实施例制得的水凝胶的展示图如图1所示。
实施例2:
第一步:称取50mg超支化聚赖氨酸(3kDa)和5g明胶加入至24.95mL超纯水和20mL甘油的混合溶液中,50℃下搅拌30分钟使其溶解。
第二步:溶解后的溶液于4℃下静置20分钟成胶。
第三步:将水凝胶制成矩形长条状、圆柱状,浸没于重量分数为20%的硫酸铵溶液中12小时,随后取出,用去离子水冲洗并擦干。
以下是本实施例制得的水凝胶的力学性能测试。在2mm/min的压缩速度下,测试水凝胶样品的压缩性能,如图2(b)所示;在10mm/min的拉伸速度下,测试水凝胶样品的拉伸性能,如图2(d)所示。可知该实施例制得的水凝胶敷料具有优异的力学性能,其在被压缩到85%应变时不发生破坏,最大应力达到2.8MPa,撤去载荷形变即可恢复;而拉伸强度可达到0.37MPa,断裂伸长率为397%,弹性模量为0.17MPa。
实施例3:
第一步:称取25mg超支化聚赖氨酸(5kDa)和5g胶原蛋白加入至34.95mL超纯水和10mL甘油的混合溶液中,60℃下搅拌1小时使其溶解。
第二步:溶解后的溶液于室温下静置60分钟成胶。
第三步:将水凝胶切成片状,浸没于重量分数为15%的柠檬酸钠溶液中12小时,随后取出,用去离子水冲洗并擦干。
以下是本实施例制得的水凝胶的抑菌圈测试。将直径为6mm的水凝胶圆片置于涂布有200μL浓度为108CFU/mL的金黄色葡萄球菌的琼脂培养基上,于37℃下培养12小时,所得抑菌圈表观图如图3所示,可以看到水凝胶周围出现了明显的抑菌圈。
实施例4:
第一步:称取50mg超支化聚赖氨酸(5kDa)和5g明胶加入至34.995mL超纯水和10mL甘油的混合溶液中,50℃下搅拌30分种使其溶解。
第二步:溶解后的溶液于4℃下静置20分钟成胶。
第三步:将水凝胶切成片状,浸没于重量分数为20%的柠檬酸钠溶液中12h,随后取出,用去离子水冲洗并擦干。
以下是本实施例制得的水凝胶的体外抗菌性能测试。将不同质量(25mg、50mg、100mg)的水凝胶与500μL浓度为106CFU/mL的金黄色葡萄球菌和大肠杆菌在37℃下共培养12小时,利用涂板法在琼脂板上对菌落数进行定量,所得抑菌率如图4所示。由图4可知,归因于所负载的超支化聚赖氨酸,本实施例制得的水凝胶对于金黄色葡萄球菌在三种用量下均达到了百分百杀灭;而对于大肠杆菌,在25mg、50mg两种用量下即有显著的杀灭效果,在100mg的用量下可以达到百分百杀灭。
对比例1:
第一步:称取50mg超支化聚赖氨酸(3kDa)和5g明胶加入至24.95mL超纯水和20mL甘油的混合溶液中,50℃下搅拌30分钟使其溶解。
第二步:溶解后的溶液于4℃下静置20分钟成胶。
第三步:将水凝胶制成矩形长条状、圆柱状。
以下是本对比例制得的水凝胶的力学性能测试。在2mm/min的压缩速度下,测试水凝胶样品的压缩性能,如图2(a)所示;在10mm/min的拉伸速度下,测试水凝胶样品的拉伸性能,如图2(c)所示。可知该水凝胶敷料力学性能差,其在被压缩到76%应变时即发生破坏,最大应力只有13.7kPa;而拉伸强度只有4.0kPa,断裂伸长率为44%,弹性模量为0.03MPa。
本发明公开的制备工艺中引入了通过霍夫迈斯特效应盐析增强这一步骤,由附图2展示的力学性能对比可见,通过本发明公开的制备方法获得的水凝胶敷料具有优异的力学性能。
对比例2:
第一步:称取5g明胶加入至35mL超纯水和10mL甘油的混合溶液中,50℃下搅拌30分种使其溶解。
第二步:溶解后的溶液于4℃下静置20分钟成胶。
第三步:将水凝胶切成片状,浸没于重量分数为20%的柠檬酸钠溶液中12h,随后取出,用去离子水冲洗并擦干。
以下是本对比例制得的水凝胶的体外抗菌性能测试。将不同质量(25mg、50mg、100mg)的水凝胶与500μL浓度为106CFU/mL的金黄色葡萄球菌和大肠杆菌在37℃下共培养12小时,利用涂板法在琼脂板上对菌落数进行定量,所得抑菌率如图4所示。由图4可知,本对比例制得的水凝胶对于金黄色葡萄球菌和大肠杆菌在三种用量下均没有抑制效果,甚至由于添加的明胶可以为细菌提供营养而导致菌落数量增加,且水凝胶的添加量越多则菌落数量增加越多。
由附图3和附图4展示的抗菌性能对比可见,本发明公开的含有HBPL的水凝胶敷料具有优异的抗菌性能。
Claims (8)
1.一种强韧抗菌水凝胶敷料,其特征在于,按重量计,每100份材料中含有5-20份主体材料、20-50份甘油、0.0025-0.3份超支化聚赖氨酸,其余为水。
2.制备如权利要求1所述的一种强韧抗菌水凝胶敷料的方法,其特征在于,制备方法如下:将主体材料和超支化聚赖氨酸加入水和甘油的混合溶液中,搅拌加热使其充分溶解;溶解后的溶液静置成胶;将固体水凝胶切成所需形状,置于重量分数为10-35%的离子溶液中浸泡8-48小时后取出,用水冲洗并擦干;所述的主体材料为以氢键作用成胶的成胶骨架材料。
3.根据权利要求2所述的一种强韧抗菌水凝胶敷料制备方法,其特征在于,所述的主体材料为壳聚糖、胶原蛋白、海藻酸盐、透明质酸、聚乙二醇、明胶、聚氨酯、聚乳酸、聚乙烯吡咯烷酮、聚乙烯醇、聚丙烯酸、卡波姆中的至少一种。
4.根据权利要求2所述的一种强韧抗菌水凝胶敷料制备方法,其特征在于,所述的超支化聚赖氨酸分子量为3-7kDa。
5.根据权利要求2所述的一种强韧抗菌水凝胶敷料制备方法,其特征在于,所述的加热条件为40-80℃下10-60分钟,静置条件为室温下30-60分钟或4℃下5-15分钟。
6.根据权利要求2所述的一种强韧抗菌水凝胶敷料制备方法,其特征在于,所述的离子溶液组成为:阴离子为酒石酸根、醋酸跟、铬酸根、柠檬酸根、硫酸根、硫酸氢根、碳酸根、碳酸氢根、磷酸二氢根、硫代硫酸根、氯离子中的一种,阳离子为铵根离子、锂离子、钾离子、钠离子、锰离子、钙离子、钡离子中的一种。
7.根据权利要求1所述的一种强韧抗菌水凝胶敷料,其特征在于,所述的超支化聚赖氨酸通过物理共混方式负载于水凝胶敷料中。
8.一种强韧抗菌水凝胶敷料,其特征在于,含有如权利要求1的敷料或2-7任一项所述方法制得的强韧抗菌水凝胶敷料,可应用于机械损伤性创面、热损伤性创面、溃疡性创面等各类创面。
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210600570.9A CN114984300B (zh) | 2022-05-30 | 2022-05-30 | 一种强韧抗菌水凝胶敷料及其制备方法 |
PCT/CN2022/097164 WO2023231050A1 (zh) | 2022-05-30 | 2022-06-06 | 一种强韧抗菌水凝胶敷料及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210600570.9A CN114984300B (zh) | 2022-05-30 | 2022-05-30 | 一种强韧抗菌水凝胶敷料及其制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN114984300A true CN114984300A (zh) | 2022-09-02 |
CN114984300B CN114984300B (zh) | 2023-08-01 |
Family
ID=83031637
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202210600570.9A Active CN114984300B (zh) | 2022-05-30 | 2022-05-30 | 一种强韧抗菌水凝胶敷料及其制备方法 |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN114984300B (zh) |
WO (1) | WO2023231050A1 (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116726241A (zh) * | 2023-08-11 | 2023-09-12 | 江苏亨瑞生物医药科技有限公司 | 一种胶原蛋白止血抗菌敷料及其制备方法 |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8946194B2 (en) | 2010-10-08 | 2015-02-03 | Board Of Regents, University Of Texas System | One-step processing of hydrogels for mechanically robust and chemically desired features |
EP2624874A4 (en) | 2010-10-08 | 2014-04-02 | Univ Texas | ADHESIVE MEMBRANE WITH ALGINATE AND HYALURONIC ACID FOR BIOMEDICAL APPLICATIONS |
Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007003028A1 (en) * | 2005-07-01 | 2007-01-11 | Kane Biotech Inc. | Antimicrobial compositions for inhibiting growth and proliferation of a microbial biofilm on medical devices |
CN103656729A (zh) * | 2013-12-11 | 2014-03-26 | 南京工业大学 | 一种基于γ-聚谷氨酸与ε-聚赖氨酸交联聚合物的水凝胶及其制备方法 |
US20160045404A1 (en) * | 2001-05-01 | 2016-02-18 | A.V. Topchiev Institute Of Petrochemical Synthesis, Russian Academy Of Sciences | Hydrogel compositions with an erodible backing member |
CN105920659A (zh) * | 2016-05-11 | 2016-09-07 | 温州医科大学 | 一种创面修复用的抑菌水凝胶敷料及制备方法 |
CN108066805A (zh) * | 2016-11-17 | 2018-05-25 | 中国科学院大连化学物理研究所 | 一种ε-聚赖氨酸仿生抑菌膜及其制备和应用 |
CN110507845A (zh) * | 2019-09-25 | 2019-11-29 | 广州沁瀚生物科技有限公司 | 生物复合透气敷料及其制备方法 |
CN110680929A (zh) * | 2019-09-18 | 2020-01-14 | 浙江大学 | 一种具有广谱活性氧清除功能的微球及其制备方法 |
CN111035803A (zh) * | 2019-11-07 | 2020-04-21 | 浙江大学 | 一种兼具抗感染及促进骨结合功能的钛植入体材料及其制备方法 |
CN111363171A (zh) * | 2020-04-02 | 2020-07-03 | 南昌大学第二附属医院 | 抗菌水凝胶及其制备方法和应用 |
US20200263203A1 (en) * | 2019-02-11 | 2020-08-20 | Florian M. Wurm | Novel eukaryotic cell transfection systems and related methods |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101982202B (zh) * | 2010-11-02 | 2013-09-18 | 华东理工大学 | 一种医用水凝胶敷料及其制备方法 |
CN113621135A (zh) * | 2018-01-31 | 2021-11-09 | 中国科学院长春应用化学研究所 | 一种支化聚氨基酸抑菌剂及应用 |
KR102083002B1 (ko) * | 2018-03-30 | 2020-02-28 | 한양대학교 에리카산학협력단 | 프로바이오틱스 함유 이중층 상처치유 드레싱제 및 그 제조방법 |
CN112190763B (zh) * | 2020-06-15 | 2022-06-10 | 南京工业大学 | 透明质酸/ε-聚赖氨酸抗菌水凝胶及其制备方法和应用 |
CN113577377B (zh) * | 2021-08-17 | 2022-03-25 | 浙江大学 | 一种活性氧消除抗菌消炎水凝胶皮肤敷料及其制备方法 |
-
2022
- 2022-05-30 CN CN202210600570.9A patent/CN114984300B/zh active Active
- 2022-06-06 WO PCT/CN2022/097164 patent/WO2023231050A1/zh unknown
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20160045404A1 (en) * | 2001-05-01 | 2016-02-18 | A.V. Topchiev Institute Of Petrochemical Synthesis, Russian Academy Of Sciences | Hydrogel compositions with an erodible backing member |
WO2007003028A1 (en) * | 2005-07-01 | 2007-01-11 | Kane Biotech Inc. | Antimicrobial compositions for inhibiting growth and proliferation of a microbial biofilm on medical devices |
CN103656729A (zh) * | 2013-12-11 | 2014-03-26 | 南京工业大学 | 一种基于γ-聚谷氨酸与ε-聚赖氨酸交联聚合物的水凝胶及其制备方法 |
CN105920659A (zh) * | 2016-05-11 | 2016-09-07 | 温州医科大学 | 一种创面修复用的抑菌水凝胶敷料及制备方法 |
CN108066805A (zh) * | 2016-11-17 | 2018-05-25 | 中国科学院大连化学物理研究所 | 一种ε-聚赖氨酸仿生抑菌膜及其制备和应用 |
US20200263203A1 (en) * | 2019-02-11 | 2020-08-20 | Florian M. Wurm | Novel eukaryotic cell transfection systems and related methods |
CN110680929A (zh) * | 2019-09-18 | 2020-01-14 | 浙江大学 | 一种具有广谱活性氧清除功能的微球及其制备方法 |
CN110507845A (zh) * | 2019-09-25 | 2019-11-29 | 广州沁瀚生物科技有限公司 | 生物复合透气敷料及其制备方法 |
CN111035803A (zh) * | 2019-11-07 | 2020-04-21 | 浙江大学 | 一种兼具抗感染及促进骨结合功能的钛植入体材料及其制备方法 |
CN111363171A (zh) * | 2020-04-02 | 2020-07-03 | 南昌大学第二附属医院 | 抗菌水凝胶及其制备方法和应用 |
Non-Patent Citations (4)
Title |
---|
ZHIJIAN YANG, ET AL: "Covalent grafting of hyperbranched poly-L-lysine on Ti-based implants achieves dual functions of antibacteria and promoted osteointegration in vivo", 《BIOMATERIALS》 * |
ZHIJIAN YANG, ET AL: "Covalent grafting of hyperbranched poly-L-lysine on Ti-based implants achieves dual functions of antibacteria and promoted osteointegration in vivo", 《BIOMATERIALS》, vol. 269, no. 2021, 16 November 2020 (2020-11-16), pages 2 - 5, XP086485513, DOI: 10.1016/j.biomaterials.2020.120534 * |
黄玉等: "生物可降解超支化聚合物的研究进展", 《高分子学报》 * |
黄玉等: "生物可降解超支化聚合物的研究进展", 《高分子学报》, no. 02, 28 February 2017 (2017-02-28) * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116726241A (zh) * | 2023-08-11 | 2023-09-12 | 江苏亨瑞生物医药科技有限公司 | 一种胶原蛋白止血抗菌敷料及其制备方法 |
CN116726241B (zh) * | 2023-08-11 | 2023-10-20 | 江苏亨瑞生物医药科技有限公司 | 一种胶原蛋白止血抗菌敷料及其制备方法 |
Also Published As
Publication number | Publication date |
---|---|
WO2023231050A1 (zh) | 2023-12-07 |
CN114984300B (zh) | 2023-08-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN114984300B (zh) | 一种强韧抗菌水凝胶敷料及其制备方法 | |
Noori et al. | Poly (vinyl alcohol)/chitosan/honey/clay responsive nanocomposite hydrogel wound dressing | |
Tsao et al. | Evaluation of chitosan/γ-poly (glutamic acid) polyelectrolyte complex for wound dressing materials | |
Archana et al. | Evaluation of chitosan nano dressing for wound healing: Characterization, in vitro and in vivo studies | |
RU2748184C2 (ru) | Композиция для раневых повязок | |
CN112370567A (zh) | 一种具有抗菌消炎功能的水凝胶活性敷料 | |
KR20170107493A (ko) | 상처 처치제용 조성물 | |
CN112480434B (zh) | 一种铜离子抗菌水凝胶及制备方法和应用 | |
CN110876815A (zh) | 一种负载富血小板血浆和抗菌肽的水凝胶及其制备方法和应用 | |
Xue et al. | Self-healing/pH-responsive/inherently antibacterial polysaccharide-based hydrogel for a photothermal strengthened wound dressing | |
Wei et al. | Facile preparation of polysaccharides-based adhesive hydrogel with antibacterial and antioxidant properties for promoting wound healing | |
CN111073001A (zh) | 一种两性葡聚糖水凝胶及应用 | |
Yi et al. | A polyphenol and ε-polylysine functionalized bacterial cellulose/PVA multifunctional hydrogel for wound healing | |
Cheng et al. | Biomass-derived ultrafast cross-linked hydrogels with double dynamic bonds for hemostasis and wound healing | |
Du et al. | Dual‐Cross‐Linked Chitosan‐Based Antibacterial Hydrogels with Tough and Adhesive Properties for Wound Dressing | |
CN113750285A (zh) | 一种复合气凝胶敷料及其制备方法 | |
Zuo et al. | Preparation and characterization of tannin-maltodextrin-polyvinyl alcohol hydrogel based on hydrogen bonding for wound healing | |
Zhang et al. | Tunicate-mimetic antibacterial hydrogel based on metal ion crosslinking and chitosan functionalization for wound healing | |
CN104497345A (zh) | 一种透明质酸-壳聚糖可降解敷料的制备方法 | |
KR20160038120A (ko) | 알지네이트 하이드로젤 및 이의 제조방법 | |
CN114404646B (zh) | CM-β-CD负载鞣酸聚丙烯酰胺型双网络抗菌水凝胶 | |
CN107185026B (zh) | 一种魔芋葡甘聚糖医用抗菌敷料的制备方法 | |
Hong et al. | Self-healing supramolecular hydrogels with antibacterial abilities for wound healing | |
Zhang et al. | A guanosine/konjac glucomannan supramolecular hydrogel with antioxidant, antibacterial and immunoregulatory properties for cutaneous wound treatment | |
CN109384943B (zh) | 抗菌性高强度壳聚糖/肝素钠离子复合物自组装水凝胶膜的制备方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |