CN114958843B - 一种可用于灰霉病防治的抑菌RNAhex及其灰霉病菌抑制剂 - Google Patents
一种可用于灰霉病防治的抑菌RNAhex及其灰霉病菌抑制剂 Download PDFInfo
- Publication number
- CN114958843B CN114958843B CN202210376296.1A CN202210376296A CN114958843B CN 114958843 B CN114958843 B CN 114958843B CN 202210376296 A CN202210376296 A CN 202210376296A CN 114958843 B CN114958843 B CN 114958843B
- Authority
- CN
- China
- Prior art keywords
- rnahex
- gray mold
- antibacterial
- botrytis cinerea
- control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003112 inhibitor Substances 0.000 title claims abstract description 11
- 230000000844 anti-bacterial effect Effects 0.000 title abstract description 14
- 241000123650 Botrytis cinerea Species 0.000 claims abstract description 24
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract description 14
- 208000015181 infectious disease Diseases 0.000 abstract description 4
- 230000004763 spore germination Effects 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 8
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- 241000219194 Arabidopsis Species 0.000 description 3
- 241001465180 Botrytis Species 0.000 description 3
- 241000227653 Lycopersicon Species 0.000 description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
- 241000208125 Nicotiana Species 0.000 description 3
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 3
- 239000003899 bactericide agent Substances 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 101150042048 DCL gene Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- 108091030071 RNAI Proteins 0.000 description 1
- 241000609666 Tuber aestivum Species 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 230000009368 gene silencing by RNA Effects 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 235000004280 healthy diet Nutrition 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/30—Special therapeutic applications
- C12N2320/32—Special delivery means, e.g. tissue-specific
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明提供一种可用于植物灰霉病防治的抑菌RNAhex分子及灰霉病菌抑制剂，其具有SEQ ID NO.1所示的RNA序列。本发明还提供一种灰霉病菌抑制剂，所述抑制剂中至少包含抑菌RNAhex分子。通过实验证明，添加本发明抑菌RNAhex分子的溶液能显著抑制灰霉病菌的孢子萌发、菌丝生长及其对植物的感染毒力，可用于植物灰霉病的防治。
Description
技术领域
本发明涉及作物灰霉病的保护和治疗，具体涉及一种可用于灰霉病防治的抑菌RNAhex及其灰霉病菌抑制剂。
背景技术
引起植物灰霉病的病原菌是灰葡萄孢（Botrytis cinerea），属于葡萄孢属（Botrytis）半知菌类。灰霉病是蔬菜和水果上为害较为严重的常见病害，主要侵害果实和茎叶，对温室大棚中作物危害尤其严重。持续高温，光照不足，通风不及时，湿度大均会引起灰霉病的严重发生，引起大量落叶落果甚至绝收，给农民造成严重损失。目前，针对灰霉病的防治方法主要使用化学农药，辅以物理防治和生物防治两种方法。由于灰霉病菌繁殖快、传播迅速和易变异等特性，伴随着大量杀菌剂的重复多年使用，灰霉病菌逐渐对各种杀菌剂产生了抗药性，使得化学杀菌剂的防治效果大大降低。并且，随着人们生活水平的逐年提高，人类追求健康饮食的诉求也越来越高。水果和蔬菜在生产过程中大量化学杀菌剂的使用，农药生产和使用过程中对环境和水资源的污染等越来越受到人们的关注。因此迫切的需要一些替代性的防治措施去防治灰霉病，所以开发新的无公害、环保型的新型农药成为科学家们首要的研究目标。
发明内容
本发明的一个目的在于提供一种可用于灰霉病防治的抑菌RNA分子，命名为RNAhex，其具有SEQ ID NO.1所示的RNA序列:
SEQ ID NO.1：
GGCUAGAGAUAAUCAACAAUCACCGGUAGAUGCCUUUUGCCUUGAGCAUCAAUUUCUACCCUACUCUCCGGAGUAGGGUAGAAAUUGAUGCUCAAGGCUUUUGGAUUCGAGUUUGUUUGCUCAACAACAACAAUCACCGGUAGAUGCCUUUUGCCUGUCUUAUUCUCAUCGUCGUGUCCCUCCGGGGACACGACGAUGAGAAUAAGACAGGCUUUUGCCAUCCGGCAUACUUGUUCAUCUACAACAAUCACCGGUAGAUGCCUUUUGCCUAUUUGACGAUAUCGCACCAUCAACGCAAGUUGAUGGUGCGAUAUCGUCAAAUAGGCUUUUGGCGCCGCAUCUCGAUGGUCAGAAUCAACAAUCACCGGUAGAUGCCUUUUGCCAACAGAAGCAACAACAUCCAAGCUCUUCGGAGCUUGGAUGUUGUUGCUUCUGUUGGCUUUUGGCCCAAGGAGAAGGUGCUGAUUGACAACAAUCACCGGUAGAUGCCUUUUGCCUCGAACAUUCUUAAGAUCCUUCGACGCAAGUCGAAGGAUCUUAAGAAUGUUCGAGGCUUUUGGUGACGGUGCAUGCUAAUCCGCUUCAAAACAGGUGAUUGAAGCGGAUUAGCAUGCACCGUCACCUUUUGGCAUCUACCGGUGAUUGUUGUCAAUCAGCACCUUCUCCUUGGGCCUUUUGGCAUCUACCGGUGAUUGUUGAUUCUGACCAUCGAGAUGCGGCGCCUUUUGGCAUCUACCGGUGAUUGUUGUAGAUGAACAAGUAUGCCGGAUGGCUUUUGGCAUCUACCGGUGAUUGUUGUUGUUGAGCAAACAAACUCGAAUCCUUUUGGCAUCUACCGGUGAUUGUUGAUUAUCUCUAGCAUCUACCGGUGACUUUUGGCAUCUACCGGAACACCUGACCGGUAGAUGCCUUUUGCCUUGAACAUCAGAACGAGGGCGACCCUCCGGGGUCGCCCUCGUUCUGAUGUUCAAGGCUUUUGUCACCGGUAGAUG。
本发明的另一个目的在于提供一种作物灰霉病菌抑制剂，所述抑制剂中至少包含RNAhex分子。
本发明所述RNA分子可抑制的病菌至少包括灰霉病菌，及其同属病菌。
在某些实施例中，所述抑制剂为包含所述RNAhex的喷雾剂，通过喷雾来实现对灰霉病菌的有效预防和治疗。
本发明的有益效果主要体现在：
1．添加RNAhex的溶液能显著抑制灰霉病菌的孢子萌发、菌丝生长及其对植物的感染毒力，可用于灰霉病的防治。
2．灰霉病菌抑制剂是基于SIGS技术防治作物病害，其主要优势在于RNA作为抑菌因子，具有环境友好、专一性强、抑菌效果良好等特性，是未来农药发展的主要方向之一。
附图说明
图1：RNAhex对灰霉病菌孢子萌发的影响；
图2：RNAhex对灰霉病菌菌丝生长的抑制效果；
图3：RNAhex在番茄、烟草和拟南芥叶片上对灰霉病菌感染的防治效果。
具体实施方式
为了实现上述目的，本发明采用如下技术方案：
一种可用于灰霉病菌防治的抑菌RNAhex及其灰霉病菌抑制剂的制备，包括以下步骤：
1．编码RNAhex的DNA模板的合成：根据本发明提供的RNAhex的序列，推导出其编码的DNA模板序列，在模板序列上游插入T7启动子序列，将这条编码RNAhex的模板DNA序列送生物公司进行化学合成获得。
2. RNAhex的体外转录合成：利用T7 RNA 体外转录试剂盒，按照操作说明获得RNAhex，将其稀释成100 ng/μL，用于灰霉病菌的防治实验。
对比例1：基于灰霉病菌的DCL基因序列设计上下游引物（F1/R1），然后分别在引物两端加入T7启动子序列，得到T7_F1/T7_R1。最后以灰霉病菌cDNA为模板，分别以T7_F1/R1和F1/T7_R1为引物扩增目的片段，得到的片段分别用于体外转录模板，得到的两条RNA混合后退火，即可得到靶向DCL的dsRNA。
对比例2：将一种不能靶向灰霉病菌的RNA作为阴性对照（NC-RNA）。
验证试验：
1、RNAhex对灰霉病菌孢子萌发的抑制作用
选取长势良好的灰霉病菌，收集分生孢子，用PD培养基配成浓度为104个/mL。取10μL浓度为4μg/μL的RNA溶液，加入到含有500μL孢子溶液的离心管中， 使RNA终浓度达到100ng/μL，26℃培养黑暗条件下培养6 h，含有相同量的dsRNA和NC-RNA作为对照，结果如图1所示。
由图1可知，与对比例相比，使用本发明的实施例，对灰霉病菌孢子的萌发抑制效率极高，所有孢子几乎没有萌发；而对比例的dsRNA对灰霉病菌孢子具有一定的抑制萌发作用，但效果远远低于本发明的抑菌RNAhex分子；NC-RNA 分子对灰霉病菌孢子的萌发没有任何抑制效果，几乎所有的孢子全部萌发。
、RNAhex对灰霉病菌菌丝生长的抑制效果
采用生长速率法，测定RNAhex对供试病原真菌的抑菌活性。将RNAhex溶液过滤除菌后，在超净工作台中溶于已冷却至50℃的PDA培养基中，配置RNAhex的终浓度为100 ng/μL的PDA培养基，倒入直径9 cm的培养皿中冷却凝固，以加入等量的dsRNA和NC-RNA的PDA培养基作为对照。
取在PDA培养基上预先培养24 h的病原菌孢子菌落，移植到培养皿的中心，放置于28℃室内倒置培养6 d，结果如图2所示。
由图2可知，与对比例相比，使用本发明的实施例RNAhex对灰霉病菌菌丝的生长抑制效率远远高于dsRNA。
、RNAhex在不同植物叶片上对灰霉病菌的防治效果
选取生长整齐一致的番茄、烟草和拟南芥三种植物，向每颗植物喷洒100 ng/μL的RNAhex溶液后培养3 d，以浓度相同的dsRNA和NC-RNA溶液作为对照。剪取大小一致的番茄、烟草和拟南芥三种植物叶片，在叶柄包裹带水棉团。在长势良好的灰霉病菌平板边缘打取菌饼，接种在叶片的背面。每片叶接1块菌饼。接种后继续在26℃、85%相对湿度的条件下培养3 d，结果如图3所示。
由图3可知，与对比例相比，使用本发明的实施例RNAhex在三种植物叶片上对灰霉病菌的防治效果远远好于对比例。
综上，表明本发明提供的抑菌RNAhex分子具有诱导触发RNAi效率更高，对灰霉病菌的感染毒力具有极为显著的抑制效果。
上述实例只是为说明本发明的应用效果，并不能以此限制本发明的保护范围。凡根据本发明的实质所做的等效变换或修饰，或利用本发明在其它植物上实施对灰霉病的防治，都应该涵盖在本发明的保护范围之内。
序列表
<110> 浙江理工大学
<120> 一种可用于灰霉病防治的抑菌RNAhex及其灰霉病菌抑制剂
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 990
<212> RNA
<213> 未知(Unknown)
<400> 1
cuagagauaa ucaacaauca ccgguagaug ccuuuugccu auauuguugu aacucucgca 60
gaucuccgga ucugcgagag uuacaacaau auaggcuuuu ggauucgagu uuguuugcuc 120
aacaacaaca aucaccggua gaugccuuuu gccugucuua uucucaucgu cgugucccuc 180
cggggacacg acgaugagaa uaagacaggc uuuuguuauu ccaagaugug uucaguaaac 240
aacaaucacc gguagaugcc uuuugccuau uugacgauau cgcaccauca acgcaaguug 300
auggugcgau aucgucaaau aggcuuuugg cgccgcaucu cgauggucag aaucaacaau 360
caccgguaga ugccuuuugc cuucuuguug caagacaacc auucucuccg gagaaugguu 420
gucuugcaac aagaaggcuu uuggcccaag gagaaggugc ugauugacaa caaucaccgg 480
uagaugccuu uugccaacag aagcaacaac auccaagcuc uucggagcuu ggauguuguu 540
gcuucuguug gcuuuuggug acggugcaug cuaauccgcu ucaaaacagg ugauugaagc 600
ggauuagcau gcaccgucac cuuuuggcau cuaccgguga uuguugucaa ucagcaccuu 660
cuccuugggc cuuuuggcau cuaccgguga uuguugauuc ugaccaucga gaugcggcgc 720
cuuuuggcau cuaccgguga uuguuguuua cugaacacau cuuggaauaa cuuuuggcau 780
cuaccgguga uuguuguugu ugagcaaaca aacucgaauc cuuuuggcau cuaccgguga 840
uuguugauua ucucuagcau cuaccgguga cuuuuggcau cuaccggaac accugaccgg 900
uagaugccuu uugccuugaa caucagaacg agggcgaccc gcaagggucg cccucguucu 960
gauguucaag gcuuuuguca ccgguagaug 990
Claims (1)
1.一种RNAhex分子在制备防治灰霉病菌抑制剂中的应用，其特征在于，RNAhex分子的RNA序列如SEQ ID NO.1所示。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210376296.1A CN114958843B (zh) | 2022-04-11 | 2022-04-11 | 一种可用于灰霉病防治的抑菌RNAhex及其灰霉病菌抑制剂 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210376296.1A CN114958843B (zh) | 2022-04-11 | 2022-04-11 | 一种可用于灰霉病防治的抑菌RNAhex及其灰霉病菌抑制剂 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114958843A CN114958843A (zh) | 2022-08-30 |
| CN114958843B true CN114958843B (zh) | 2023-12-19 |
Family
ID=82977594
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210376296.1A Active CN114958843B (zh) | 2022-04-11 | 2022-04-11 | 一种可用于灰霉病防治的抑菌RNAhex及其灰霉病菌抑制剂 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114958843B (zh) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019167338A (ja) * | 2018-03-23 | 2019-10-03 | 国立大学法人山口大学 | 植物病害の抑制剤及び植物病害の抑制方法 |
| CN112662675A (zh) * | 2021-01-22 | 2021-04-16 | 浙江理工大学 | 一种RNA抑菌剂phasiRNA5及作物病菌抑制剂 |
| CN112695035A (zh) * | 2021-01-22 | 2021-04-23 | 浙江理工大学 | 一种RNA抑菌剂miRNA157d-3p及作物病菌抑制剂 |
| CN112899171A (zh) * | 2021-04-02 | 2021-06-04 | 河南科技大学 | 白囊耙齿菌ll210及其应用、生防菌剂 |
-
2022
- 2022-04-11 CN CN202210376296.1A patent/CN114958843B/zh active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019167338A (ja) * | 2018-03-23 | 2019-10-03 | 国立大学法人山口大学 | 植物病害の抑制剤及び植物病害の抑制方法 |
| CN112662675A (zh) * | 2021-01-22 | 2021-04-16 | 浙江理工大学 | 一种RNA抑菌剂phasiRNA5及作物病菌抑制剂 |
| CN112695035A (zh) * | 2021-01-22 | 2021-04-23 | 浙江理工大学 | 一种RNA抑菌剂miRNA157d-3p及作物病菌抑制剂 |
| CN112899171A (zh) * | 2021-04-02 | 2021-06-04 | 河南科技大学 | 白囊耙齿菌ll210及其应用、生防菌剂 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114958843A (zh) | 2022-08-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101643705B (zh) | 一种抑制番茄灰霉病的粘帚霉菌菌株 | |
| CN108342330B (zh) | 一株具有广谱抗菌性能的长枝木霉及其应用 | |
| CN110669677B (zh) | 一株布雷正青霉菌菌株及其应用 | |
| CN114921347A (zh) | 一种防治大豆拟茎点种腐病的生防菌及其应用 | |
| CN110438017B (zh) | 枝孢瓶霉菌株lj1及其应用 | |
| CN114276957B (zh) | 一种具有防治多种植物病原真菌效果的生防菌株及其应用 | |
| CN111363691B (zh) | 一株多粘类芽孢杆菌及其应用 | |
| CN114958843B (zh) | 一种可用于灰霉病防治的抑菌RNAhex及其灰霉病菌抑制剂 | |
| CN112662675A (zh) | 一种RNA抑菌剂phasiRNA5及作物病菌抑制剂 | |
| CN109055265B (zh) | 一种生防菌及其在农作物蔓枯病防治方面的应用 | |
| CN114292759B (zh) | 一株具有防治烟草连作障碍作用的尖孢镰刀菌 | |
| CN114807133B (zh) | 一种可用于灰霉病防治的RNAsqu纳米分子及一种作物病菌抑制剂 | |
| CN107142211B (zh) | 一种杀根结线虫的桔绿木霉Snef1910及代谢产物及应用 | |
| CN114190404B (zh) | 一种烟草靶斑病的生物防治方法及应用 | |
| CN116686834A (zh) | 槲皮素在制备用于防治植物枯萎病的杀菌剂中的应用 | |
| CN116083297A (zh) | 一种防治草莓根腐病的微生物菌剂及其制备方法 | |
| CN114854742B (zh) | 一种用于防治灰霉病的RNA纳米分子RNAtet及其灰霉病抑制剂 | |
| CN114854741B (zh) | 一种可用于灰霉病防治的RNA纳米分子RNAtri及其制备方法和应用 | |
| CN114854743B (zh) | 一种可用于灰霉病防治的RNApen纳米分子及其应用 | |
| CN114644986A (zh) | 一种深绿木霉及其在防治三七病害发生中的应用 | |
| CN114885945A (zh) | 一种诱导烟草防治黑胫病的方法 | |
| CN114634878A (zh) | 一种复合微生物制剂及其在防治根结线虫中的应用 | |
| CN111454848A (zh) | 一种雅致放射毛霉gd48及其发酵产物、菌剂与应用 | |
| CN116836813B (zh) | 一种生防菌横梗霉及其应用 | |
| CN113512502B (zh) | 一种内生真菌及其在防治腐烂病中的应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |