CN114958628A - 一种蔗泥专用厌氧活性生物发酵剂的制备方法 - Google Patents
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Abstract
一种蔗泥专用厌氧活性生物发酵剂的制备方法,该方法是以发酵基材、BP正烷酵母粉、BP石油酵母粉、ESSO细菌干粉、纸浆酵母粉、酿酒酵母、小麦粉和黄豆粉为原料,先经过发酵制成接种母菌液、然后再加入活化剂活化、从而制成厌氧活性生物发酵剂的方法,其制备方法包括下述步骤:A配制发酵基材、B配制接种母菌液、C制备厌氧活性生物发酵剂。本方法二次污染小、安全环保,经济效益明显,可为蔗泥的处理提供一条新的途径,用该法制得的蔗泥专用厌氧活性生物发酵剂对蔗泥进行厌氧发酵,可克服常规好氧堆肥工艺开放型堆肥工艺产生大量臭气散发导致环境的污染;所得到的蔗泥发酵组合物,可代替部分玉米、麦糠用作配制各种畜禽、水产鱼类生物饲料。
Description
技术领域
本发明涉及一种生物菌剂的制备方法,尤其涉及一种蔗泥专用厌氧活性生物发酵剂的制备方法。
背景技术
目前我国甘蔗制糖大部分采用亚硫酸法制糖,制糖过程中产生的蔗泥残留的有机质、蛋白质和发热量都比较高,具有较高的利用价值,长期来制糖产生的蔗泥大多随意丢弃,一是得不到充分利用,二是在自然界中腐烂发酵,散发恶臭对环境造成极大危害;目前虽有部分企业对其作为生物质有机肥进行资源化综合利用,但采用的是好氧工艺堆肥工艺,在好氧堆肥工艺过程中会散发恶臭气体,仍然对环境造成严重危害。
发明内容
本发明旨在解决现有技术的上述缺陷,提供一种蔗泥专用厌氧活性生物发酵剂的制备方法,用其制得的蔗泥专用厌氧活性生物发酵剂对蔗泥进行厌氧发酵,可极大程度缓解这种恶臭气体的散发,从而克服常规好氧堆肥工艺开放型堆肥工艺的缺点,为生物法处理蔗泥工艺提供一种环保型生产工艺提供保障。
本发明采取的技术方案是:一种蔗泥专用厌氧活性生物发酵剂的制备方法, 它是以发酵基材、BP正烷酵母粉、BP石油酵母粉、ESSO细菌干粉、纸浆酵母粉、酿酒酵母、小麦粉和黄豆粉为原料经过发酵制成接种母菌液后再加入活化剂活化制成厌氧活性生物发酵剂的方法,其制备方法包括下述步骤:
A. 配制发酵基材:
取蔗渣与洁净水,按照蔗渣:洁净水=1:5的质量比混合后,蒸煮2小时后静置将温度降至28~38℃,形成发酵基材;
B.配制接种母菌液:
B1.配制混合物Ⅰ
按照质量份比取发酵基材20份、BP正烷酵母粉1份、BP石油酵母粉1份、ESSO细菌干粉1份、纸浆酵母粉1份、酿酒酵母1份、小麦粉3份和黄豆粉2份,将其均匀混合得到混合物Ⅰ;
B2.通过厌氧发酵制备混合物Ⅱ
将步骤B1配制的混合物Ⅰ置于酸碱度4.0~6.0PH、温度28~38℃的缺氧环境下进行厌氧发酵5~7天,在发酵过程中使各种微生物进行无性繁殖杂交,最终形成具有专门分解蔗渣和蔗糖的高效微生物种群的混合物Ⅱ;
B3.过滤提取接种母菌液
将上述经厌氧发酵的混合物Ⅱ过滤后得到含有高效微生物种群的接种母菌液,滤渣返回进行二次发酵循环套用;
C.制备厌氧活性生物发酵剂:
C1.配制活化剂浆
取纤维素酶干粉、半纤维素酶干粉、葡萄糖苷酶干粉、米曲霉干粉,按干粉与水1:5的比例分别兑水稀释至浆液状,制成活化剂浆、分别为:纤维素酶粉浆、半纤维素酶粉浆、葡萄糖苷酶粉浆、米曲霉粉浆;
C2.配制接种母菌液与活化剂浆的混合物Ⅲ
按质量比取步骤B3制成的接种母菌液30份、步骤C1制成的纤维素酶粉浆3份、半纤维素酶粉浆2份、葡萄糖苷酶粉浆1份、米曲霉粉浆1份均匀混合成混合物Ⅲ;
C3. 混合物Ⅲ活化
将C2步骤制得的混合物Ⅲ静态遮光密封48小时进行活化,得到厌氧活性生物发酵菌剂,该厌氧活性生物发酵菌剂中的菌落总数≥200亿/克。
其进一步的技术方案是:在进行步骤B2时:当发酵温度高于20℃时,发酵时间为5天,当发酵温度低于20℃时,发酵时间为6天,当发酵温度低于10℃时,发酵时间为7天。
更进一步:在进行步骤C3. 混合物Ⅲ活化时,按混合物Ⅲ:营养剂=1:0.2的比例加入营养剂,所述营养剂是按废糖蜜:酒饼=1:0.5的比例混合而成的混合物。
由于采取上述技术方案,本发明之一种蔗泥专用厌氧活性生物发酵剂的制备方法
具有下述有益效果:
(1)本发明之一种蔗泥专用厌氧型活性生物菌剂的制备方法,以利用蔗渣为基础,将蔗渣与水配成浆液,对其进行高温蒸煮,进一步软化纤维和释放蔗渣中的糖分,所得到发酵基材更利于微生物的消化,然后在厌氧条件下筛选出对糖液和木质素具有特定分解功能的接种母菌液,通过活化剂与接种母菌液进行活化处理,活化剂中的纤维素酶干粉、半纤维素酶干粉、葡萄糖苷酶干粉是纤维素和糖分的分解酶,具有相互协同作用,用本蔗泥专用厌氧型活性生物菌剂与蔗泥进行厌氧发酵所得到的蔗泥发酵组合物,能够有效将滤泥中残留的糖分和木质素分解为单胃动物够能吸收消化的蛋白和其他营养物质,提高蔗泥的附加值,最终产物
中含有大量益生菌衍生的菌体蛋白、活性肽、生物酶、有机酸等,促进动物生长,增强动物免疫功能,改善肉产品品质,可代替部分玉米、麦糠用作配制各种畜禽、水产鱼类生物饲料;
(2)纤维素酶干粉、半纤维素酶干粉、葡萄糖苷酶干粉属厌氧型发酵微生物,与好氧型微生物不同,它是在厌氧或缺氧条件下对蔗泥中的糖分和木质素进行转化,用该法制得的蔗泥专用厌氧活性生物发酵剂对蔗泥进行厌氧发酵,可克服常规好氧堆肥工艺开放型堆肥工艺产生大量臭气散发的缺点,极大程度缓解恶臭气体的散发,因而可避免好氧堆肥导致环境的污染;
(3)本发明二次污染小、安全环保,经济效益明显,可为蔗泥的处理提供一条新的途径。
下面结合附图和实施例对本发明之一种蔗泥专用厌氧型活性生物菌剂的制备方法的技术特征做进一步的说明。
附图说明
图1为本发明之一种蔗泥专用厌氧活性生物发酵剂的制备方法工艺流程图。
具体实施方式
一种蔗泥专用厌氧活性生物发酵剂的制备方法,该方法是以发酵基材、BP正烷酵母粉、BP石油酵母粉、ESSO细菌干粉、纸浆酵母粉、酿酒酵母、小麦粉和黄豆粉为原料,先经过发酵制成接种母菌液、然后再加入活化剂活化、从而制成厌氧活性生物发酵剂的方法,其制备方法包括下述步骤:
A. 配制发酵基材:
取蔗渣与洁净水,按照蔗渣:洁净水=1:5的质量比混合后,蒸煮2小时后静置将温度降至28~38℃,形成发酵基材;
B.配制接种母菌液:
B1.配制混合物Ⅰ
按照质量份比取步骤A制得的发酵基材20份、BP正烷酵母粉1份、BP石油酵母粉1份、ESSO细菌干粉1份、纸浆酵母粉1份、酿酒酵母1份、小麦粉3份和黄豆粉2份,将其均匀混合得到混合物Ⅰ;
B2.通过厌氧发酵制备混合物Ⅱ
将步骤B1配制的混合物Ⅰ置于酸碱度4.0~6.0PH、温度28~38℃的缺氧环境下进行厌氧发酵5~7天,在发酵过程中使各种微生物进行无性繁殖杂交,最终形成具有专门分解蔗渣和蔗糖的高效微生物种群的混合物Ⅱ;
B3.过滤混合物Ⅱ提取接种母菌液
将上述经厌氧发酵的混合物Ⅱ过滤后得到含有高效微生物种群的接种母菌液,滤渣返回进行二次发酵循环套用;
C.制备厌氧活性生物发酵剂:
C1.配制活化剂浆
取纤维素酶干粉、半纤维素酶干粉、葡萄糖苷酶干粉、米曲霉干粉,按干粉与水1:5的比例分别兑水稀释至浆液状,制成活化剂浆、分别为:纤维素酶粉浆、半纤维素酶粉浆、葡萄糖苷酶粉浆、米曲霉粉浆;
C2.配制接种母菌液与活化剂浆的混合物Ⅲ
按质量比取步骤B3制成的接种母菌液30份、步骤C1制成的纤维素酶粉浆3份、半纤维素酶粉浆2份、葡萄糖苷酶粉浆1份、米曲霉粉浆1份均匀混合成混合物Ⅲ;
C3.混合物Ⅲ活化
将C2步骤制得的混合物Ⅲ静态遮光密封48小时进行活化,得到厌氧活性生物发酵菌剂,该厌氧活性生物发酵菌剂中的菌落总数≥200亿/克。
在进行步骤B2时:当发酵温度高于20℃时,发酵时间为5天,当发酵温度低于20℃时,发酵时间为6天,当发酵温度低于10℃时,发酵时间为7天。
在进行步骤C3混合物Ⅲ活化时,按混合物Ⅲ:营养剂=1:0.2的比例加入营养剂,所述营养剂是按废糖蜜:酒饼=1:0.5的比例混合而成的混合物。
Claims (3)
1.一种蔗泥专用厌氧活性生物发酵剂的制备方法,其特征在于:它是以发酵基材、BP正烷酵母粉、BP石油酵母粉、ESSO细菌干粉、纸浆酵母粉、酿酒酵母、小麦粉和黄豆粉为原料经过发酵制成接种母菌液,然后再加入活化剂活化制成厌氧活性生物发酵剂的方法,其制备方法包括下述步骤:
A.配制发酵基材:
取蔗渣与洁净水,按照蔗渣:洁净水=1:5的质量比混合后,蒸煮2小时后静置将温度降至28~38℃,形成发酵基材;
B.配制接种母菌液:
B1.配制混合物Ⅰ
按照质量份比取发酵基材20份、BP正烷酵母粉1份、BP石油酵母粉1份、ESSO细菌干粉1份、纸浆酵母粉1份、酿酒酵母1份、小麦粉3份和黄豆粉2份,将其均匀混合得到混合物Ⅰ;
B2.厌氧发酵制备混合物Ⅱ
将步骤B1配制的混合物Ⅰ置于酸碱度4.0~6.0PH、环境温度28~38℃的缺氧环境下进行厌氧发酵5~7天,在发酵过程中使各种微生物进行无性繁殖杂交,最终形成具有专门分解蔗渣和蔗糖的高效微生物种群的混合物Ⅱ;
B3.过滤提取接种母菌液
将上述经厌氧发酵的混合物Ⅱ过滤后得到含有高效微生物种群的接种母菌液,滤渣返回进行二次发酵循环套用;
C.制备厌氧活性生物发酵剂:
C1.配制活化剂浆
取纤维素酶干粉、半纤维素酶干粉、葡萄糖苷酶干粉、米曲霉干粉,按干粉与水1:5的比例分别兑水稀释至浆液状,制成活化剂浆、分别为:纤维素酶粉浆、半纤维素酶粉浆、葡萄糖苷酶粉浆、米曲霉粉浆;
C2.配制接种母菌液与活化剂浆的混合物Ⅲ
按质量比取步骤B3制成的接种母菌液30份、步骤C1制成的纤维素酶粉浆3份、半纤维素酶粉浆2份、葡萄糖苷酶粉浆1份、米曲霉粉浆1份均匀混合成混合物Ⅲ;
C3.混合物Ⅲ活化
将C2步骤制得的混合物Ⅲ静态遮光密封48小时进行活化,得到厌氧活性生物发酵菌剂,该厌氧活性生物发酵菌剂中的菌落总数≥200亿/克。
2.如权利要求1所述的一种蔗泥专用厌氧活性生物发酵剂的制备方法,其特征在于:在进行步骤B2时:当发酵温度高于20℃时,发酵时间为5天,当发酵温度低于20℃时,发酵时间为6天,当发酵温度低于10℃时,发酵时间为7天。
3.如权利要求1所述的一种蔗泥专用厌氧活性生物发酵剂的制备方法,其特征在于:在进行步骤C3. 混合物Ⅲ活化时,按混合物Ⅲ:营养剂=1:0.2的比例加入营养剂,所述营养剂是按废糖蜜:酒饼=1:0.5的比例混合而成的混合物。
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