CN114949988A - Extracellular protein purification device of lactic acid bacteria - Google Patents

Extracellular protein purification device of lactic acid bacteria Download PDF

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Publication number
CN114949988A
CN114949988A CN202210673896.4A CN202210673896A CN114949988A CN 114949988 A CN114949988 A CN 114949988A CN 202210673896 A CN202210673896 A CN 202210673896A CN 114949988 A CN114949988 A CN 114949988A
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CN
China
Prior art keywords
thick bamboo
filter screen
purification
section
lactic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210673896.4A
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Chinese (zh)
Inventor
裴金金
张洪霞
张永贵
王金泽
刘哲
王宇临
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shaanxi Mobo Information Technology Co ltd
Shaanxi University of Technology
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Shaanxi Mobo Information Technology Co ltd
Shaanxi University of Technology
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Publication date
Application filed by Shaanxi Mobo Information Technology Co ltd, Shaanxi University of Technology filed Critical Shaanxi Mobo Information Technology Co ltd
Priority to CN202210673896.4A priority Critical patent/CN114949988A/en
Publication of CN114949988A publication Critical patent/CN114949988A/en
Pending legal-status Critical Current

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D35/00Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
    • B01D35/06Filters making use of electricity or magnetism
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D35/00Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
    • B01D35/16Cleaning-out devices, e.g. for removing the cake from the filter casing or for evacuating the last remnants of liquid
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05Stirrers
    • B01F27/11Stirrers characterised by the configuration of the stirrers
    • B01F27/19Stirrers with two or more mixing elements mounted in sequence on the same axis
    • B01F27/191Stirrers with two or more mixing elements mounted in sequence on the same axis with similar elements
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/90Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with paddles or arms 

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a lactobacillus extracellular protein purification device which comprises a purification cylinder, wherein a sieve hopper is arranged on the inner wall of the purification cylinder, a filter screen is embedded in the side wall of the sieve hopper, a mixing rod is rotatably arranged in the purification cylinder, a mixing blade is fixedly connected to the mixing rod, an impurity collecting cylinder is arranged in the purification cylinder and is positioned below the sieve hopper, and the top of the purification cylinder is connected with a feeding pipe. This extracellular protein purification device of lactic acid bacteria, the material is sent into in the purification section of thick bamboo through the pan feeding pipe, driving motor drive mixing rod is rotatory, the messenger mixes the leaf and carries out hybrid processing to the material, impurity in this in-process filter screen is to the material carries out the separation, with the purity that promotes the material, and because the magnetism ball is inhaled mutually with the magnetism of filter screen, make the magnetism ball rotate near the filter screen when, the filter screen drives the swing of axle that resets, then make the filter screen can take place the shake when the miscellaneous function of performance sieve, make the impurity of separation fall to in the miscellaneous section of thick bamboo of collection, not only make the filter effect better, it is showing to receive miscellaneous effect moreover.

Description

Extracellular protein purification device of lactic acid bacteria
Technical Field
The invention relates to the technical field of extraction of extracellular protease of lactic acid bacteria, in particular to a purification device of extracellular protein of lactic acid bacteria.
Background
When extracting the extracellular protease of the lactobacillus, the steps of crushing, medicament precipitation, separation and filtration and the like are needed, and the separation and filtration step is to achieve the purpose of purifying the extracellular protease of the lactobacillus. The publication number is CN212925016U and the name is a protease purification device, which comprises a support frame, a shell fixed on the support frame and a first cylinder fixed on the shell; a centrifugal filtering mechanism for performing centrifugal filtration on the solution containing the protease is arranged in the first cylinder; a centrifugal power mechanism connected with the centrifugal filtering mechanism is arranged in the shell; a fine filtering mechanism is also arranged in the shell; a liquid power mechanism is further arranged in the shell and used for sending liquid centrifugally filtered in the first cylinder into the fine filtering mechanism; the top of first barrel is provided with the top cap, and is equipped with the inside feed liquor pipe of intercommunication first barrel on the top cap, and is equipped with first valve on the feed liquor pipe. This protease purification device can provide good filter effect for protease purification process, and the structure is ingenious, and the filter screen is removable, and the filter screen is changed portably. The aforesaid prior art scheme still has the defect, if when clearing up accumulation impurity, needs the filter screen of repeatability to dismantle, and the operation is inconvenient, and easily causes the wearing and tearing of filter screen installation department, can not see impurity off fast, and then reduces purification effect and purification efficiency. Therefore, it is necessary to design a purification device for extracellular proteins of lactic acid bacteria so as to solve the problems mentioned above.
Disclosure of Invention
The invention aims to provide a lactobacillus extracellular protein purification device to solve the problems in the prior art.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides an extracellular protein purification device of lactic acid bacteria, includes a purification section of thick bamboo, the sieve fill is installed to the inner wall of a purification section of thick bamboo, and the lateral wall that the sieve was fought inlays and establishes the filter screen, the mixing rod is installed to purification section of thick bamboo internal rotation, and fixedly connected with mixes the leaf on the mixing rod, be equipped with the miscellaneous section of thick bamboo of collection in the purification section of thick bamboo, and the miscellaneous section of thick bamboo of collection is located the below that the sieve was fought.
Preferably, the top of the purification cylinder is connected with a feeding pipe, the bottom of the purification cylinder is connected with a discharging pipe, and the discharging pipe is provided with a control valve.
Preferably, the top fixed mounting of purification section of thick bamboo has driving motor, and driving motor's output fixed connection mixing rod, and one of them tip fixedly connected with magnetism ball of mixing the leaf, the filter screen is the magnetism filter screen, and magnetism filter screen and magnetism ball magnetism attract mutually.
Preferably, the movable groove has been seted up to the lateral wall of sieve fill, and the inner wall in movable groove rotates and installs the axle that resets, filter screen fixed mounting is on the axle that resets, the epaxial cover that resets has the torsional spring, and the both ends of torsional spring fixed connection respectively on movable groove inner wall and the axle that resets.
Preferably, a cleaning opening is formed in the side face of the purifying cylinder, a cleaning cover is detachably mounted in the cleaning opening through a screw, a moving strip is movably arranged in the purifying cylinder, an electric telescopic rod is fixedly mounted on the moving strip, and an output end of the electric telescopic rod is fixedly connected with a impurity collecting cylinder.
Preferably, the inner wall of a purification section of thick bamboo rotates and installs the lead screw, and the one end of lead screw extends the purification section of thick bamboo outside and fixedly connected with carousel, the inner wall of a purification section of thick bamboo still fixedly connected with and the parallel guide arm of lead screw, and remove a thread and install on the lead screw, remove a slidable mounting on the guide arm, the size of the miscellaneous section of thick bamboo of collection is less than the size of clearance mouth.
Preferably, the bottom end of the mixing rod extends into the impurity collecting barrel and is fixedly connected with a cutting piece, and a liquid outlet grid mesh is embedded in the side wall of the impurity collecting barrel.
Compared with the prior art, the invention has the beneficial effects that:
(1) this extracellular protein purification device of lactic acid bacteria, the material is sent into in the purification section of thick bamboo through the pan feeding pipe, driving motor drive mixing rod is rotatory, the messenger mixes the leaf and carries out hybrid processing to the material, impurity in this in-process filter screen is to the material carries out the separation, with the purity that promotes the material, and because the magnetism ball is inhaled mutually with the magnetism of filter screen, make the magnetism ball rotate near the filter screen when, the filter screen drives the swing of axle that resets, then make the filter screen can take place the shake when the miscellaneous function of performance sieve, make the impurity of separation fall to in the miscellaneous section of thick bamboo of collection, not only make the filter effect better, it is showing to receive miscellaneous effect moreover.
(2) This extracellular protein purification device of lactic acid bacteria, the miscellaneous in-process of collection, because the bottom of hybrid lever extends in the miscellaneous section of thick bamboo of collection, the messenger cuts the piece and breaks up impurity, with the filtrating effect of the miscellaneous section of thick bamboo of enhancement collection, start electric telescopic handle after the miscellaneous completion of collection, make electric telescopic handle drive the miscellaneous section of thick bamboo of collection and move down, cut the piece and break away from the miscellaneous section of thick bamboo of collection, rotate the carousel afterwards and make the lead screw rotatory, thereby make the removal strip drive the miscellaneous section of thick bamboo of collection and move towards clearance mouth, open the clearance lid, move to clearance mouth until the miscellaneous section of thick bamboo of collection, so that the impurity in the miscellaneous section of thick bamboo of collection is fallen in the clearance, keep clean, so that next work, it is very convenient to collect miscellaneous row of miscellaneous.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of another state of FIG. 1 according to the present invention;
FIG. 3 is a perspective view of a structured collection vessel of the present invention;
FIG. 4 is a perspective view of a sieve hopper of the present invention;
FIG. 5 is a half-sectional view of a sieve hopper of the present construction;
FIG. 6 is an enlarged view of portion A of the block diagram of the present invention shown in FIG. 5;
FIG. 7 is a top view of a structural lead screw and guide bar of the present invention;
FIG. 8 is a cross-sectional view of a structured collection vessel of the present invention.
In the figure: 1. a purification cartridge; 2. a screen bucket; 3. a filter screen; 4. a sundry collecting vessel; 5. a mixing rod; 6. mixing leaves; 7. a feeding pipe; 8. a discharge pipe; 9. a control valve; 10. a drive motor; 11. a magnetic ball; 12. cleaning the cover; 13. a moving bar; 14. an electric telescopic rod; 15. a screw rod; 16. a guide bar; 17. cutting the sheet; 18. discharging the liquid grid; 19. a movable groove; 20. a reset shaft; 21. a torsion spring.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-8, the present invention provides the following embodiments:
example one
The utility model provides an extracellular protein purification device of lactic acid bacteria, includes a purification section of thick bamboo 1, the sieve is installed to the inner wall of a purification section of thick bamboo 1 and is fought 2, and the lateral wall that the sieve was fought 2 inlays and establishes filter screen 3, and mixing rod 5 is installed to 1 internal rotation of a purification section of thick bamboo, and fixedly connected with mixes leaf 6 on the mixing rod 5, is equipped with an album miscellaneous section of thick bamboo 4 in the purification section of thick bamboo 1, and an album miscellaneous section of thick bamboo 4 is located the below that the sieve was fought 2.
Further, the top of the purification cylinder 1 is connected with a feeding pipe 7, the bottom of the purification cylinder 1 is connected with a discharging pipe 8, and the discharging pipe 8 is provided with a control valve 9.
Further, a driving motor 10 is fixedly installed at the top of the purification cylinder 1, the output end of the driving motor 10 is fixedly connected with the mixing rod 5, the end part of one of the mixing blades 6 is fixedly connected with a magnetic ball 11, the filter screen 3 is a magnetic filter screen, and the magnetic filter screen and the magnetic ball 11 are magnetically attracted.
Further, movable groove 19 has been seted up to the lateral wall of sieve fill 2, and the inner wall of movable groove 19 rotates and installs reset shaft 20, and filter screen 3 fixed mounting has torsional spring 21 on reset shaft 20, the cover has on reset shaft 20, and the both ends of torsional spring 21 are fixed connection respectively on movable groove 19 inner wall and reset shaft 20.
The specific implementation manner of this embodiment is: the material is sent into in the purification section of thick bamboo through the pan feeding pipe, driving motor drive mixing rod is rotatory, the messenger mixes the leaf and carries out hybrid processing to the material, impurity in this in-process filter screen is to the material carries out the separation, in order to promote the purity of material, and because the magnetism ball is inhaled with the magnetism of filter screen mutually, make the magnetism ball rotate near the filter screen when, the filter screen drives the swing of axle that resets, then make the filter screen can take place to shake when the miscellaneous function of performance sieve, make the impurity of separation fall to in the miscellaneous section of thick bamboo of collection, not only make the filter effect better, it is showing to receive miscellaneous effect moreover.
Example two
The difference from the first embodiment is that the following contents are also included:
a clearance mouth has been seted up to the side of a purification section of thick bamboo 1, and has removable clearance lid 12 of installing through the screw in the clearance mouth, and the activity is equipped with the removal strip 13 in a purification section of thick bamboo 1, and removes strip 13 and go up fixed mounting have electric telescopic handle 14, electric telescopic handle 14's the miscellaneous section of thick bamboo 4 of output fixed connection collection.
Further, the inner wall of the purification cylinder 1 is rotatably provided with a screw rod 15, one end of the screw rod 15 extends to the outside of the purification cylinder 1 and is fixedly connected with a turntable, the inner wall of the purification cylinder 1 is also fixedly connected with a guide rod 16 parallel to the screw rod 15, a moving strip 13 is arranged on the screw rod 15 in a threaded manner, the moving strip 13 is arranged on the guide rod 16 in a sliding manner, and the size of the impurity collecting cylinder 4 is smaller than that of the cleaning port.
Further, the bottom end of the mixing rod 5 extends into the impurity collecting barrel 4 and is fixedly connected with a cutting sheet 17, and a liquid outlet grid 18 is embedded in the side wall of the impurity collecting barrel 4.
In this embodiment: the miscellaneous in-process of collection, because the bottom of hybrid pole extends in the miscellaneous section of thick bamboo of collection, make cut the piece and break up impurity, with the filtrating effect of strengthening the miscellaneous section of thick bamboo of collection, start electric telescopic handle after the miscellaneous completion of collection, make electric telescopic handle drive the miscellaneous section of thick bamboo of collection and move down, cut the piece and break away from the miscellaneous section of thick bamboo of collection, rotate the carousel afterwards and make the lead screw rotatory, thereby make the removal strip drive the miscellaneous section of thick bamboo of collection and move towards clearance mouth, open the clearance lid, move to clearance mouth until the miscellaneous section of thick bamboo of collection, so that the impurity in the miscellaneous section of thick bamboo of collection of clearance, keep clean, so that the work of next time, it is very convenient to collect miscellaneous row of miscellaneous.
Those not described in detail in this specification are within the skill of the art.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof.

Claims (7)

1. The utility model provides a lactic acid bacteria extracellular protein purification device, includes purification section of thick bamboo (1), its characterized in that: the inner wall of a purification section of thick bamboo (1) is installed the sieve and is fought (2), and the lateral wall of sieve fill (2) inlays and establishes filter screen (3), mixing rod (5) are installed to purification section of thick bamboo (1) internal rotation, and fixedly connected with mixes leaf (6) on mixing rod (5), be equipped with in purification section of thick bamboo (1) and collect miscellaneous section of thick bamboo (4), and collect miscellaneous section of thick bamboo (4) and be located the below that the sieve was fought (2).
2. The apparatus for purifying extracellular protein of lactic acid bacteria according to claim 1, wherein: the top of purification section of thick bamboo (1) is connected with pan feeding pipe (7), and the bottom of purification section of thick bamboo (1) is connected with discharging pipe (8), install control valve (9) on discharging pipe (8).
3. The apparatus for purifying extracellular protein of lactic acid bacteria according to claim 1, wherein: the top fixed mounting of purification section of thick bamboo (1) has driving motor (10), and output fixed connection mixing rod (5) of driving motor (10), tip fixedly connected with magnetism ball (11) that one of them mixes leaf (6), filter screen (3) are the magnetism filter screen, and magnetism filter screen and magnetism ball (11) magnetism attract mutually.
4. The apparatus for purifying extracellular protein of lactic acid bacteria according to claim 3, wherein: activity groove (19) have been seted up to the lateral wall of sieve fill (2), and the inner wall of activity groove (19) rotates and installs reset shaft (20), filter screen (3) fixed mounting is on reset shaft (20), the cover has torsional spring (21) on reset shaft (20), and the both ends difference fixed connection of torsional spring (21) is on activity groove (19) inner wall and reset shaft (20).
5. The apparatus for purifying extracellular protein of lactic acid bacteria according to claim 1, wherein: the side of a purification section of thick bamboo (1) has been seted up the clearance mouth, and has been installed clearance lid (12) through the screw is removable in the clearance mouth, purification section of thick bamboo (1) internalization is equipped with removes strip (13), and removes strip (13) and go up fixed mounting and have electric telescopic handle (14), the miscellaneous section of thick bamboo (4) of output fixed connection collection of electric telescopic handle (14).
6. An extracellular protein purification device of lactic acid bacteria according to claim 5, characterized in that: the inner wall of a purification section of thick bamboo (1) rotates and installs lead screw (15), and the one end of lead screw (15) extends purification section of thick bamboo (1) outer and fixedly connected with carousel, the inner wall of a purification section of thick bamboo (1) is fixedly connected with guide arm (16) parallel with lead screw (15) still, and removes strip (13) screw thread and install on lead screw (15), remove strip (13) slidable mounting on guide arm (16), the size of the miscellaneous section of thick bamboo of collection (4) is little than the size of clearance mouth.
7. The apparatus for purifying extracellular protein of lactic acid bacteria according to claim 1, wherein: the bottom end of the mixing rod (5) extends into the impurity collecting barrel (4) and is fixedly connected with a cutting piece (17), and a liquid outlet grid net (18) is embedded in the side wall of the impurity collecting barrel (4).
CN202210673896.4A 2022-06-15 2022-06-15 Extracellular protein purification device of lactic acid bacteria Pending CN114949988A (en)

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CN202210673896.4A CN114949988A (en) 2022-06-15 2022-06-15 Extracellular protein purification device of lactic acid bacteria

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Application Number Priority Date Filing Date Title
CN202210673896.4A CN114949988A (en) 2022-06-15 2022-06-15 Extracellular protein purification device of lactic acid bacteria

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1190123A (en) * 1997-09-18 1999-04-06 Matsushita Electric Ind Co Ltd Filter
CN111777235A (en) * 2020-08-10 2020-10-16 南京九胜揽天科技有限公司 Landfill leachate membrane filtration concentrated solution treatment facility
CN112962760A (en) * 2021-02-22 2021-06-15 乐清市纳琦环保科技有限公司 Municipal drainage ditch with vegetation planting layer
CN214781040U (en) * 2021-04-27 2021-11-19 安钢集团信阳钢铁有限责任公司 Water collecting device for sintering waste heat softener
CN215560261U (en) * 2021-06-24 2022-01-18 东北农业大学 Extracellular protease purification device of lactic acid bacteria
CN215842284U (en) * 2021-07-28 2022-02-18 东营市利民无害化处理有限公司 Purification treatment device for filtered tail gas
CN216470983U (en) * 2021-05-07 2022-05-10 河北昂星建材科技有限公司 Production waste recovery device for external wall insulation field

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1190123A (en) * 1997-09-18 1999-04-06 Matsushita Electric Ind Co Ltd Filter
CN111777235A (en) * 2020-08-10 2020-10-16 南京九胜揽天科技有限公司 Landfill leachate membrane filtration concentrated solution treatment facility
CN112962760A (en) * 2021-02-22 2021-06-15 乐清市纳琦环保科技有限公司 Municipal drainage ditch with vegetation planting layer
CN214781040U (en) * 2021-04-27 2021-11-19 安钢集团信阳钢铁有限责任公司 Water collecting device for sintering waste heat softener
CN216470983U (en) * 2021-05-07 2022-05-10 河北昂星建材科技有限公司 Production waste recovery device for external wall insulation field
CN215560261U (en) * 2021-06-24 2022-01-18 东北农业大学 Extracellular protease purification device of lactic acid bacteria
CN215842284U (en) * 2021-07-28 2022-02-18 东营市利民无害化处理有限公司 Purification treatment device for filtered tail gas

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