CN215560261U - Extracellular protease purification device of lactic acid bacteria - Google Patents

Extracellular protease purification device of lactic acid bacteria Download PDF

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Publication number
CN215560261U
CN215560261U CN202121414526.6U CN202121414526U CN215560261U CN 215560261 U CN215560261 U CN 215560261U CN 202121414526 U CN202121414526 U CN 202121414526U CN 215560261 U CN215560261 U CN 215560261U
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China
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outer shell
filter
extracellular protease
lactic acid
acid bacteria
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CN202121414526.6U
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孙方达
孔保华
刘骞
夏秀芳
陈倩
王浩
王辉
崔元永菊
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Northeast Agricultural University
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Northeast Agricultural University
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Abstract

The utility model discloses a lactobacillus extracellular protease purification device which is stably placed on the ground of a working area; the feeding device comprises an outer shell, wherein a feeding hole is connected to the upper left of the outer shell, and a servo motor is fixed to the upper right of the outer shell through a bolt; the screw rod is rotatably connected to the outer shell by a bearing, and the upper end of the screw rod which is distributed in an inverted T-shaped structure is connected with the output end of the servo motor; the waste material groove is formed in the middle of the lower part of the outer shell; the method comprises the following steps: and the mounting frame is in threaded connection with the outer side of the screw rod. This extracellular protease purification device of lactic acid bacteria, the drive is carried the seat and is rotated, utilizes the annular transport seat of circle to carry the material, and utilizes the bottom of carrying the seat slope form to increase the transport time of material, and the rotation of seat is carried in the cooperation, carries the even comprehensive transport of material on the filter to the upper end from the filter begins to filter, increases filter time and avoids the material single face to accumulate and carry out the purification on the filter, improves purification efficiency.

Description

Extracellular protease purification device of lactic acid bacteria
Technical Field
The utility model relates to the technical field of extraction of extracellular protease of lactic acid bacteria, in particular to a purification device of extracellular protease of lactic acid bacteria.
Background
When extracting the extracellular protease of the lactobacillus, the steps of crushing, medicament precipitation, separation and filtration and the like are needed, and the separation and filtration step is to achieve the purpose of purifying the extracellular protease of the lactobacillus.
The publication number is CN212925016U and the name is a protease purification device, which comprises a support frame, a shell fixed on the support frame and a first cylinder fixed on the shell; a centrifugal filtering mechanism for performing centrifugal filtration on the solution containing the protease is arranged in the first cylinder; a centrifugal power mechanism connected with the centrifugal filtering mechanism is arranged in the shell; a fine filtering mechanism is also arranged in the shell; a liquid power mechanism is further arranged in the shell and used for sending liquid centrifugally filtered in the first cylinder into the fine filtering mechanism; the top of first barrel is provided with the top cap, and is equipped with the inside feed liquor pipe of intercommunication first barrel on the top cap, and is equipped with first valve on the feed liquor pipe. This protease purification device can provide good filter effect for protease purification process, and the structure is ingenious, and the filter screen is removable, and the filter screen is changed portably.
The above prior art solutions have the following drawbacks: when clearing up accumulation impurity, need the filter screen of repeatability to dismantle, the operation is inconvenient, and easily causes the wearing and tearing of filter screen installation department, can not the omnidirectional carry the material filtering mechanism in, the purification is filtered in the single face accumulation, reduces purification effect and purification efficiency.
Therefore, we propose a purification apparatus for extracellular protease of lactic acid bacteria so as to solve the above-mentioned problems.
Disclosure of Invention
The utility model aims to provide a lactobacillus extracellular protease purification device, which solves the problems that in the prior market, when accumulated impurities are cleaned, a filter screen needs to be repeatedly disassembled, the operation is inconvenient, the abrasion of a filter screen mounting part is easy to cause, materials cannot be conveyed into a filter mechanism in an all-around manner, the materials are accumulated on a single surface to be filtered and purified, and the purification effect and the purification efficiency are reduced.
In order to achieve the purpose, the utility model provides the following technical scheme: a lactobacillus extracellular protease purification device which is stably placed on the ground of a working area;
the feeding device comprises an outer shell, wherein a feeding hole is connected to the upper left of the outer shell, and a servo motor is fixed to the upper right of the outer shell through a bolt;
the screw rod is rotatably connected to the outer shell by a bearing, and the upper end of the screw rod which is distributed in an inverted T-shaped structure is connected with the output end of the servo motor;
the waste material groove is formed in the middle of the lower part of the outer shell;
the method comprises the following steps:
the mounting frame is in threaded connection with the outer side of the screw rod, a driving motor is fixed to an inner bolt of the mounting frame, and the output end of the driving motor is connected with a rotating rod;
the sliding rod is arranged above the left side of the mounting rack;
the supporting frame is connected to the outer side above the rotating rod, a conveying seat is installed at one end, away from the rotating rod, of the supporting frame, and a discharging port is formed in one end, away from the supporting frame, of the conveying seat;
the collecting tank is arranged in the lower portion of the outer shell, the bottom side surface of the collecting tank is distributed in an inclined mode, and a filter plate is arranged on the inner side wall of the outer shell.
Preferably, the rotating rod is provided with a connecting rod and a sealing plate:
the connecting rods are symmetrically arranged on the outer side below the rotating rod, and one end of each connecting rod, which is far away from the rotating rod, is provided with a cleaning plate;
and the sealing plate is rotatably connected to the lower end of the rotating rod.
Preferably, the main section of mounting bracket is for falling "protruding" shape structure, and the biggest external diameter of mounting bracket top is less than carry the internal diameter of seat, just carry the section of bowing of seat to the ring shape structure, and carry the inside bottom side of seat and personally submit the slope form and distribute, this design can guarantee that the material is carried to the avris position of carrying the seat along with carrying the seat, prolongs the travel time of material, follows the rotation of carrying the seat and carries the material comprehensively.
Preferably, the slide bar with the lead screw is parallel to each other, and be "T" shape structure the slide bar runs through the upside of shell body and with sliding connection about the shell body, the removal position of mounting bracket is injectd in the removal of this usable slide bar of design, guarantees the vertical removal of mounting bracket, avoids the mounting bracket to take place to rotate the phenomenon.
Preferably, the clearance board with the mutual parallel distribution of filter, and the main section of filter is the slope form and distributes, just the minimum of filter with the peak of dirty tank coincides each other, and the rotation of usable clearance board of this design clears up the filter, avoids impurity accumulation on the filter to block up.
Preferably, the maximum outer diameter of the sealing plate is larger than the inner diameter of the waste trough, the upper side surface of the sealing plate is distributed in an inclined manner, the sealing plate can be used for blocking the waste trough, and the material purification time is prolonged.
Compared with the prior art, the utility model has the beneficial effects that: the lactobacillus extracellular protease purification device comprises a purification device,
(1) the conveying seat is driven to rotate, materials are conveyed by the annular conveying seat, the conveying time of the materials is prolonged by the inclined bottom of the conveying seat, the materials are uniformly and comprehensively conveyed onto the filter plate by the aid of rotation of the conveying seat, the materials are filtered from the upper end of the filter plate, the filtering time is prolonged, single sides of the materials are prevented from being accumulated on the filter plate to be purified, and the purifying efficiency is improved;
(2) the control closing plate reciprocates, the control closing plate encloses stifled to the dirty tank, can be long when the purification of accuse material, avoid material purification time too short and cause the waste, and the rotation of cooperation cleaning plate, sustainable to clearing up the filter, avoid the filter to block up, and when the closing plate removed the enclosure to the dirty tank, impurity on the filter can enter into the dirty tank and concentrate the collection, need not to dismantle filter mechanism, easy operation is convenient.
Drawings
FIG. 1 is a schematic view of the main sectional structure of the present invention;
FIG. 2 is an enlarged view of a portion a of FIG. 1 according to the present invention;
FIG. 3 is a schematic view of a top-down structure of the delivery seat of the present invention;
FIG. 4 is a schematic top view of the swivel lever of the present invention;
FIG. 5 is a schematic view of a cross-sectional view of a collection trough according to the present invention.
In the figure: 1. an outer housing; 2. a feed inlet; 3. a servo motor; 4. a screw rod; 5. a mounting frame; 6. a slide bar; 7. a drive motor; 8. a rotating rod; 9. a support frame; 10. a conveying seat; 11. a feeding port; 12. a connecting rod; 13. cleaning the plate; 14. a filter plate; 15. a sealing plate; 16. a waste chute; 17. and (4) collecting the tank.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides a technical solution: a lactobacillus extracellular protease purification device which is stably placed on the ground of a working area;
a feed inlet 2 is connected to the left upper part of the outer shell 1, and a servo motor 3 is fixed to the right upper bolt of the outer shell 1;
the screw rod 4 is rotatably connected to the outer shell 1 by a bearing, and the upper end of the screw rod 4 which is distributed in an inverted T-shaped structure is connected with the output end of the servo motor 3;
a waste chute 16 arranged in the middle of the lower part of the outer shell 1;
the method comprises the following steps:
the mounting frame 5 is in threaded connection with the outer side of the screw rod 4, a driving motor 7 is fixed to the inner bolt of the mounting frame 5, and the output end of the driving motor 7 is connected with a rotating rod 8;
the sliding rod 6 is arranged above the left side of the mounting frame 5;
the supporting frame 9 is connected to the outer side above the rotating rod 8, a conveying seat 10 is installed at one end, far away from the rotating rod 8, of the supporting frame 9, and a discharging opening 11 is formed in one end, far away from the supporting frame 9, of the conveying seat 10;
the collecting tank 17 is arranged in the lower portion of the outer shell 1, the bottom side surface of the collecting tank 17 is distributed in an inclined manner, and the filtering plate 14 is arranged on the inner side wall of the outer shell 1.
The main section of the mounting frame 5 is in an inverted convex structure, the maximum outer diameter above the mounting frame 5 is smaller than the inner diameter of the conveying seat 10, the depression section of the conveying seat 10 is in a circular ring structure, and the bottom side surface inside the conveying seat 10 is in inclined distribution.
The material is carried in the shell body 1 from feed inlet 2, fall into earlier and carry seat 10 in, carry seat 10 along the slope form and enter into feed opening 11, fall on filter 14 after that, driving motor 7 external power supply, control driving motor 7 and drive the 8 rotations of bull stick 8 that the output is connected, 8 control support frames of bull stick 9 rotate, support frame 9 drives the transport seat 10 rotation of end installation, carry the filter 14 with the material is even comprehensive, and filter from the upper end of filter 14 begins the whereabouts.
The rotating rod 8 is provided with a connecting rod 12 and a sealing plate 15: the connecting rods 12 are symmetrically arranged on the outer side below the rotating rod 8, and one ends of the connecting rods 12 far away from the rotating rod 8 are provided with cleaning plates 13; and a sealing plate 15 rotatably connected to the lower end of the rotary rod 8. The slide bar 6 and the screw rod 4 are arranged in parallel, and the slide bar 6 with a T-shaped structure penetrates through the upper side of the outer shell 1 and is connected with the outer shell 1 in a vertical sliding manner. The cleaning plate 13 and the filter plate 14 are arranged in parallel, the main section of the filter plate 14 is distributed in an inclined shape, and the lowest point of the filter plate 14 and the highest point of the waste material tank 16 are overlapped. The maximum outer diameter of the sealing plate 15 is larger than the inner diameter of the waste chute 16, and the upper side surface of the sealing plate 15 is distributed in an inclined shape.
The material is filtered and purified by using the filter plate 14, the purified liquid can enter the collecting groove 17 to be collected, meanwhile, the rotating rod 8 controls the connecting rod 12 to rotate, the cleaning plate 13 arranged at the control end part of the connecting rod 12 rotates, the cleaning plate 13 scrapes impurities on the filter plate 14 to avoid the blockage of the filter plate 14, after a period of purification, the servo motor 3 can be powered on, the servo motor 3 is controlled to rotate positively, the filter plate 14 connected with the control output end of the servo motor 3 rotates, the filter plate 14 controls the mounting frame 5 in threaded connection with the outer side to move upwards, at the moment, the slide rod 6 on the mounting frame 5 slides on the outer shell 1 to ensure the stable vertical movement of the mounting frame 5, thereby the rotating rod 8 is controlled to drive the sealing plate 15 to move upwards, the blockage of the sealing plate 15 to the waste material groove 16 is gradually removed, the impurities on the filter plate 14 can fall into the waste material groove 16 to be collected in a concentrated manner, the servo motor 3 is controlled to rotate reversely, so that the screw rod 4 can be driven to rotate reversely, the screw rod 4 controls the mounting rack 5 to move downwards, and the sealing plate 15 is controlled to move downwards to block the waste trough 16.
The working principle is as follows: when using this extracellular protease purification device of lactic acid bacteria, firstly, the user is earlier in the steady work area of placing of whole device shown in fig. 1, carry the material in outer shell body 1 from feed inlet 2, driving motor 7 external power supply, carry seat 10 to rotate on carrying filter 14 with the even comprehensive transport of material, clearance plate 13 rotates and scrapes the impurity on the filter 14, servo motor 3 corotation, closing plate 15 moves up and removes the encirclement to waste chute 16 gradually, servo motor 3 reversal, closing plate 15 moves down and encloses and blocks waste chute 16, the fluid-discharge outlet in the later stage can be opened on the left of collecting vat 17, collect the purification liquid, open the sealing door below waste chute 16, clear up impurity, the content that does not make detailed description in this specification belongs to the prior art that professional technical personnel know in this field.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the utility model can be made, and equivalents and modifications of some features of the utility model can be made without departing from the spirit and scope of the utility model.

Claims (6)

1. A lactobacillus extracellular protease purification device comprises an outer shell, wherein a feed inlet is connected to the upper left of the outer shell, and a servo motor is fixed to the upper right of the outer shell through a bolt;
the screw rod is rotatably connected to the outer shell by a bearing, and the upper end of the screw rod which is distributed in an inverted T-shaped structure is connected with the output end of the servo motor;
the waste material groove is formed in the middle of the lower part of the outer shell;
the method is characterized in that: the method comprises the following steps:
the mounting frame is in threaded connection with the outer side of the screw rod, a driving motor is fixed to an inner bolt of the mounting frame, and the output end of the driving motor is connected with a rotating rod;
the sliding rod is arranged above the left side of the mounting rack;
the supporting frame is connected to the outer side above the rotating rod, a conveying seat is installed at one end, away from the rotating rod, of the supporting frame, and a discharging port is formed in one end, away from the supporting frame, of the conveying seat;
the collecting tank is arranged in the lower portion of the outer shell, the bottom side surface of the collecting tank is distributed in an inclined mode, and a filter plate is arranged on the inner side wall of the outer shell.
2. The apparatus for purifying extracellular protease of lactic acid bacteria according to claim 1, wherein: be provided with connecting rod and closing plate on the bull stick:
the connecting rods are symmetrically arranged on the outer side below the rotating rod, and one end of each connecting rod, which is far away from the rotating rod, is provided with a cleaning plate;
and the sealing plate is rotatably connected to the lower end of the rotating rod.
3. The apparatus for purifying extracellular protease of lactic acid bacteria according to claim 1, wherein: the main section of the mounting frame is of an inverted convex structure, the maximum outer diameter above the mounting frame is smaller than the inner diameter of the conveying seat, the depression section of the conveying seat is of a circular ring structure, and the bottom side surface inside the conveying seat is in inclined distribution.
4. The apparatus for purifying extracellular protease of lactic acid bacteria according to claim 1, wherein: the slide bar with the lead screw is parallel to each other, and is "T" shape structure the slide bar runs through the upside of shell body and with shell body sliding connection from top to bottom.
5. The apparatus for purifying extracellular protease of lactic acid bacteria according to claim 2, wherein: the cleaning plate and the filter plate are distributed in parallel, the main section of the filter plate is distributed in an inclined shape, and the lowest point of the filter plate and the highest point of the waste tank coincide with each other.
6. The apparatus for purifying extracellular protease of lactic acid bacteria according to claim 2, wherein: the maximum outer diameter of the sealing plate is larger than the inner diameter of the waste material groove, and the upper side surface of the sealing plate is distributed in an inclined shape.
CN202121414526.6U 2021-06-24 2021-06-24 Extracellular protease purification device of lactic acid bacteria Active CN215560261U (en)

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Application Number Priority Date Filing Date Title
CN202121414526.6U CN215560261U (en) 2021-06-24 2021-06-24 Extracellular protease purification device of lactic acid bacteria

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Application Number Priority Date Filing Date Title
CN202121414526.6U CN215560261U (en) 2021-06-24 2021-06-24 Extracellular protease purification device of lactic acid bacteria

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CN215560261U true CN215560261U (en) 2022-01-18

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114949988A (en) * 2022-06-15 2022-08-30 陕西理工大学 Extracellular protein purification device of lactic acid bacteria

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114949988A (en) * 2022-06-15 2022-08-30 陕西理工大学 Extracellular protein purification device of lactic acid bacteria

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