CN114929897A - 制备用于多肽测序的富集样品的方法 - Google Patents

制备用于多肽测序的富集样品的方法 Download PDF

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Publication number
CN114929897A
CN114929897A CN202080091893.9A CN202080091893A CN114929897A CN 114929897 A CN114929897 A CN 114929897A CN 202080091893 A CN202080091893 A CN 202080091893A CN 114929897 A CN114929897 A CN 114929897A
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polypeptide
polypeptides
molecules
amino acid
oxa
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Chinese (zh)
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布莱恩·瑞德
马修·戴尔
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Quantum Si Inc
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Quantum Si Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202080091893.9A 2019-10-28 2020-10-28 制备用于多肽测序的富集样品的方法 Pending CN114929897A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962926897P 2019-10-28 2019-10-28
US62/926,897 2019-10-28
PCT/US2020/057638 WO2021086901A1 (en) 2019-10-28 2020-10-28 Methods of preparing an enriched sample for polypeptide sequencing

Publications (1)

Publication Number Publication Date
CN114929897A true CN114929897A (zh) 2022-08-19

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US (1) US20210148921A1 (enExample)
EP (1) EP4045684A1 (enExample)
JP (1) JP2023500477A (enExample)
KR (1) KR20220108053A (enExample)
CN (1) CN114929897A (enExample)
AU (1) AU2020374879A1 (enExample)
BR (1) BR112022008092A2 (enExample)
CA (1) CA3159357A1 (enExample)
MX (1) MX2022005091A (enExample)
WO (1) WO2021086901A1 (enExample)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113512598A (zh) * 2020-12-07 2021-10-19 上海仁度生物科技股份有限公司 百日咳杆菌的实时荧光核酸恒温扩增检测试剂盒及其专用引物和探针
CN115798576A (zh) * 2023-02-06 2023-03-14 中国医学科学院北京协和医院 一种预测克雷伯氏菌属对亚胺培南敏感性的系统及方法
CN118109435A (zh) * 2024-04-12 2024-05-31 天津法莫西生物医药科技有限公司 一种o-甲基转移酶突变体及其制备方法与应用
CN118173169A (zh) * 2024-03-28 2024-06-11 杭州洛兮医学检验实验室有限公司 一种检测肺炎支原体及其23S rRNA基因耐药突变的生物信息分析方法
CN118600027A (zh) * 2024-06-17 2024-09-06 广东生态工程职业学院 一种与鹦鹉精子活力和受精率相关的miRNA及其应用
CN119185551A (zh) * 2024-11-06 2024-12-27 浙江安吉吉检医疗科技有限公司 一种基于细菌dna旋转酶凝聚体的细菌药物靶点及药物

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI863941B (zh) 2018-11-15 2024-12-01 美商寬騰矽公司 用於蛋白質定序之方法及組合物
EP4051903A1 (en) 2019-10-29 2022-09-07 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices
AU2021276522A1 (en) 2020-05-20 2023-01-05 Quantum-Si Incorporated Methods and compositions for protein sequencing
CN114437993B (zh) * 2022-03-10 2023-07-18 中国农业科学院农产品加工研究所 罗伊氏乳杆菌及其在制备预防猫腹泻制剂中的应用
WO2024086832A1 (en) * 2022-10-21 2024-04-25 Quantum-Si Incorporated Polypeptide cleaving reagents and uses thereof
CN116726156A (zh) * 2023-03-20 2023-09-12 西北农林科技大学 一种口服酵母介导的产气荚膜梭菌α毒素重组DNA疫苗

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US9566335B1 (en) * 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents

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WO2010044892A1 (en) * 2008-10-17 2010-04-22 President And Fellows Of Harvard College Diagnostic method based on large scale identification of post-translational modification of proteins
DK3011051T3 (en) * 2013-06-21 2019-04-23 Sequenom Inc Method for non-invasive evaluation of genetic variations
WO2016193980A1 (en) * 2015-06-03 2016-12-08 Bar Ilan University Methods and kits for detection and quantification of large-scale post translational modifications of proteins
MX2020004559A (es) * 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
WO2020023488A1 (en) * 2018-07-23 2020-01-30 Board Of Regents, The University Of Texas System Single molecule sequencing identification of post-translational modifications on proteins
EP3966824A1 (en) * 2019-06-12 2022-03-16 Quantum-Si Incorporated Techniques for protein identification using machine learning and related systems and methods
US20210354134A1 (en) * 2020-04-22 2021-11-18 Quantum-Si Incorporated Sample preparation for sequencing

Patent Citations (2)

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WO2007123708A2 (en) * 2006-03-31 2007-11-01 Epitome Biosystems, Inc. Post translational modification pattern analysis
US9566335B1 (en) * 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents

Non-Patent Citations (2)

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PETER A. JEKEL ET AL.: "Use of endoproteinase Lys-C from lysobacter enzymogenes in protein sequence analysis", 《ANALYTICAL BIOCHEMISTRY》, vol. 134, no. 2, 15 October 1983 (1983-10-15), pages 347 - 354, XP024818588, DOI: 10.1016/0003-2697(83)90308-1 *
TOVE BOSTRÖM: "High-throughput protein analysis using mass spectrometry-based methods", KTH ROYAL INSTITUTE OF TECHNOLOGY SCHOOL OF BIOTECHNOLOGY, STOCKHOLM 2014, 22 October 2014 (2014-10-22), pages 135 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113512598A (zh) * 2020-12-07 2021-10-19 上海仁度生物科技股份有限公司 百日咳杆菌的实时荧光核酸恒温扩增检测试剂盒及其专用引物和探针
CN113512598B (zh) * 2020-12-07 2024-03-22 上海仁度生物科技股份有限公司 百日咳杆菌的实时荧光核酸恒温扩增检测试剂盒及其专用引物和探针
CN115798576A (zh) * 2023-02-06 2023-03-14 中国医学科学院北京协和医院 一种预测克雷伯氏菌属对亚胺培南敏感性的系统及方法
CN118173169A (zh) * 2024-03-28 2024-06-11 杭州洛兮医学检验实验室有限公司 一种检测肺炎支原体及其23S rRNA基因耐药突变的生物信息分析方法
CN118109435A (zh) * 2024-04-12 2024-05-31 天津法莫西生物医药科技有限公司 一种o-甲基转移酶突变体及其制备方法与应用
CN118600027A (zh) * 2024-06-17 2024-09-06 广东生态工程职业学院 一种与鹦鹉精子活力和受精率相关的miRNA及其应用
CN119185551A (zh) * 2024-11-06 2024-12-27 浙江安吉吉检医疗科技有限公司 一种基于细菌dna旋转酶凝聚体的细菌药物靶点及药物

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EP4045684A1 (en) 2022-08-24
CA3159357A1 (en) 2021-05-06
JP2023500477A (ja) 2023-01-06
KR20220108053A (ko) 2022-08-02
US20210148921A1 (en) 2021-05-20
BR112022008092A2 (pt) 2022-07-12
WO2021086901A1 (en) 2021-05-06
AU2020374879A1 (en) 2022-06-02
MX2022005091A (es) 2022-08-11

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