CA3159357A1 - Methods of preparing an enriched sample for polypeptide sequencing - Google Patents

Methods of preparing an enriched sample for polypeptide sequencing

Info

Publication number
CA3159357A1
CA3159357A1 CA3159357A CA3159357A CA3159357A1 CA 3159357 A1 CA3159357 A1 CA 3159357A1 CA 3159357 A CA3159357 A CA 3159357A CA 3159357 A CA3159357 A CA 3159357A CA 3159357 A1 CA3159357 A1 CA 3159357A1
Authority
CA
Canada
Prior art keywords
polypeptides
polypeptide
amino acid
oxa
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3159357A
Other languages
English (en)
French (fr)
Inventor
Brian Reed
Matthew Dyer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantum Si Inc
Original Assignee
Quantum Si Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum Si Inc filed Critical Quantum Si Inc
Publication of CA3159357A1 publication Critical patent/CA3159357A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3159357A 2019-10-28 2020-10-28 Methods of preparing an enriched sample for polypeptide sequencing Pending CA3159357A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962926897P 2019-10-28 2019-10-28
US62/926,897 2019-10-28
PCT/US2020/057638 WO2021086901A1 (en) 2019-10-28 2020-10-28 Methods of preparing an enriched sample for polypeptide sequencing

Publications (1)

Publication Number Publication Date
CA3159357A1 true CA3159357A1 (en) 2021-05-06

Family

ID=73544294

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3159357A Pending CA3159357A1 (en) 2019-10-28 2020-10-28 Methods of preparing an enriched sample for polypeptide sequencing

Country Status (10)

Country Link
US (1) US20210148921A1 (enExample)
EP (1) EP4045684A1 (enExample)
JP (1) JP2023500477A (enExample)
KR (1) KR20220108053A (enExample)
CN (1) CN114929897A (enExample)
AU (1) AU2020374879A1 (enExample)
BR (1) BR112022008092A2 (enExample)
CA (1) CA3159357A1 (enExample)
MX (1) MX2022005091A (enExample)
WO (1) WO2021086901A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI863941B (zh) 2018-11-15 2024-12-01 美商寬騰矽公司 用於蛋白質定序之方法及組合物
EP4051903A1 (en) 2019-10-29 2022-09-07 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices
AU2021276522A1 (en) 2020-05-20 2023-01-05 Quantum-Si Incorporated Methods and compositions for protein sequencing
CN113512598B (zh) * 2020-12-07 2024-03-22 上海仁度生物科技股份有限公司 百日咳杆菌的实时荧光核酸恒温扩增检测试剂盒及其专用引物和探针
CN114437993B (zh) * 2022-03-10 2023-07-18 中国农业科学院农产品加工研究所 罗伊氏乳杆菌及其在制备预防猫腹泻制剂中的应用
WO2024086832A1 (en) * 2022-10-21 2024-04-25 Quantum-Si Incorporated Polypeptide cleaving reagents and uses thereof
CN115798576B (zh) * 2023-02-06 2023-06-02 中国医学科学院北京协和医院 一种预测克雷伯氏菌属对亚胺培南敏感性的系统及方法
CN116726156A (zh) * 2023-03-20 2023-09-12 西北农林科技大学 一种口服酵母介导的产气荚膜梭菌α毒素重组DNA疫苗
CN118173169B (zh) * 2024-03-28 2024-11-26 杭州洛兮医学检验实验室有限公司 一种检测肺炎支原体及其23S rRNA基因耐药突变的生物信息分析方法
CN118109435B (zh) * 2024-04-12 2025-07-15 天津法莫西生物医药科技有限公司 一种o-甲基转移酶突变体及其制备方法与应用
CN118600027B (zh) * 2024-06-17 2025-04-18 广东生态工程职业学院 一种与鹦鹉精子活力和受精率相关的miRNA及其应用
CN119185551B (zh) * 2024-11-06 2025-05-27 浙江安吉吉检医疗科技有限公司 一种基于细菌dna旋转酶凝聚体的细菌药物靶点及药物

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2496294A1 (en) 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
WO2007123708A2 (en) * 2006-03-31 2007-11-01 Epitome Biosystems, Inc. Post translational modification pattern analysis
WO2010044892A1 (en) * 2008-10-17 2010-04-22 President And Fellows Of Harvard College Diagnostic method based on large scale identification of post-translational modification of proteins
US9566335B1 (en) * 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
DK3011051T3 (en) * 2013-06-21 2019-04-23 Sequenom Inc Method for non-invasive evaluation of genetic variations
WO2016193980A1 (en) * 2015-06-03 2016-12-08 Bar Ilan University Methods and kits for detection and quantification of large-scale post translational modifications of proteins
MX2020004559A (es) * 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
WO2020023488A1 (en) * 2018-07-23 2020-01-30 Board Of Regents, The University Of Texas System Single molecule sequencing identification of post-translational modifications on proteins
EP3966824A1 (en) * 2019-06-12 2022-03-16 Quantum-Si Incorporated Techniques for protein identification using machine learning and related systems and methods
US20210354134A1 (en) * 2020-04-22 2021-11-18 Quantum-Si Incorporated Sample preparation for sequencing

Also Published As

Publication number Publication date
EP4045684A1 (en) 2022-08-24
CN114929897A (zh) 2022-08-19
JP2023500477A (ja) 2023-01-06
KR20220108053A (ko) 2022-08-02
US20210148921A1 (en) 2021-05-20
BR112022008092A2 (pt) 2022-07-12
WO2021086901A1 (en) 2021-05-06
AU2020374879A1 (en) 2022-06-02
MX2022005091A (es) 2022-08-11

Similar Documents

Publication Publication Date Title
US20210148921A1 (en) Methods of preparing an enriched sample for polypeptide sequencing
US20240344122A1 (en) Methods of single-cell sequencing
US20210148922A1 (en) Methods of single-polypeptide sequencing and reconstruction
US20210147474A1 (en) Methods of preparing samples for multiplex polypeptide sequencing
US20230250437A1 (en) Oligonucleotide probes and uses thereof
US20240102084A1 (en) Compositions and methods for detection of a nucleic acid
AU2017271579B2 (en) Oligonucleotide probes and uses thereof
US20150011397A1 (en) Methods for quantitative determination of multiple proteins in complex mixtures
CN119716077A (zh) 采用核酸编码和/或标签进行分析的试剂盒
CA3038502A1 (en) Oligonucleotide probes and uses thereof

Legal Events

Date Code Title Description
W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-1-1-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20250825

U13 Renewal or maintenance fee not paid

Free format text: ST27 STATUS EVENT CODE: N-1-6-U10-U13-U300 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: DEEMED ABANDONED - FAILURE TO RESPOND TO MAINTENANCE FEE NOTICE

Effective date: 20251027

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: N-6-6-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20251224

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: N-6-6-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20251231

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: N-6-6-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20260202

B12 Application deemed to be withdrawn, abandoned or lapsed

Free format text: ST27 STATUS EVENT CODE: N-6-6-B10-B12-B301 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: DEEMED ABANDONED - FAILURE TO RESPOND TO A REQUEST FOR EXAMINATION NOTICE

Effective date: 20260402

U13 Renewal or maintenance fee not paid

Free format text: ST27 STATUS EVENT CODE: N-1-6-U10-U13-U300 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: DEEMED ABANDONED - FAILURE TO RESPOND TO MAINTENANCE FEE NOTICE

Effective date: 20260428

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: N-6-6-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20260703