CN114929887A - 单个多肽测序和重建的方法 - Google Patents

单个多肽测序和重建的方法 Download PDF

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Publication number
CN114929887A
CN114929887A CN202080090914.5A CN202080090914A CN114929887A CN 114929887 A CN114929887 A CN 114929887A CN 202080090914 A CN202080090914 A CN 202080090914A CN 114929887 A CN114929887 A CN 114929887A
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China
Prior art keywords
polypeptide
amino acid
molecules
sample
polypeptides
Prior art date
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Pending
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CN202080090914.5A
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English (en)
Chinese (zh)
Inventor
马修·戴尔
布莱恩·瑞德
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Quantum Si Inc
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Quantum Si Inc
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Publication of CN114929887A publication Critical patent/CN114929887A/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202080090914.5A 2019-10-28 2020-10-28 单个多肽测序和重建的方法 Pending CN114929887A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962927005P 2019-10-28 2019-10-28
US62/927,005 2019-10-28
US201962940968P 2019-11-27 2019-11-27
US62/940,968 2019-11-27
PCT/US2020/057669 WO2021086918A1 (en) 2019-10-28 2020-10-28 Methods of single-polypeptide sequencing and reconstruction

Publications (1)

Publication Number Publication Date
CN114929887A true CN114929887A (zh) 2022-08-19

Family

ID=73476267

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080090914.5A Pending CN114929887A (zh) 2019-10-28 2020-10-28 单个多肽测序和重建的方法

Country Status (10)

Country Link
US (1) US20210148922A1 (https=)
EP (1) EP4051806A1 (https=)
JP (1) JP2023501932A (https=)
KR (1) KR20220108056A (https=)
CN (1) CN114929887A (https=)
AU (1) AU2020376814A1 (https=)
BR (1) BR112022007937A2 (https=)
CA (1) CA3159362A1 (https=)
MX (1) MX2022005094A (https=)
WO (1) WO2021086918A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201715684D0 (en) 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
KR20250152680A (ko) 2017-12-29 2025-10-23 노틸러스 서브시디어리, 인크. 단백질 식별을 위한 디코딩 접근법
AU2019380606B2 (en) 2018-11-15 2026-04-23 Quantum-Si Incorporated Methods and compositions for protein sequencing
US12011716B2 (en) 2019-10-29 2024-06-18 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices
EP4143579A2 (en) 2020-05-20 2023-03-08 Quantum-si Incorporated Methods and compositions for protein sequencing
CA3227872A1 (en) * 2021-09-09 2023-03-16 James Henry JOLY Characterization and localization of protein modifications
AU2022422112A1 (en) 2021-12-22 2024-08-01 Trustees Of Boston University Bodipy-based dyes for digital spatial proteomics
JP2025538856A (ja) * 2022-10-21 2025-12-02 クアンタム－エスアイ インコーポレイテッド ポリペプチド切断試薬及びその使用
WO2025169569A1 (ja) * 2024-02-08 2025-08-14 コニカミノルタ株式会社 核酸アプタマーの配列の決定方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010065322A1 (en) * 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
US20100311098A1 (en) * 2008-01-15 2010-12-09 Universiteit Utrecht Holding B.V. Method for determining the amino acid sequence of peptides

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2496294A1 (en) 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
WO2010065531A1 (en) * 2008-12-01 2010-06-10 Robi David Mitra Single molecule protein screening
US9435810B2 (en) * 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
CA3208970A1 (en) * 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
CA3081446A1 (en) * 2017-10-31 2019-05-09 Encodia, Inc. Methods and compositions for polypeptide analysis
JP7390027B2 (ja) * 2017-10-31 2023-12-01 エンコディア， インコーポレイテッド 核酸エンコーディングおよび／または標識を使用する解析のためのキット
CA3110800A1 (en) * 2018-07-12 2020-01-16 Board Of Regents, The University Of Texas System Molecular neighborhood detection by oligonucleotides
KR20210045449A (ko) * 2018-08-17 2021-04-26 리제너론 파아마슈티컬스, 인크. 새로운 단백질 서열분석 방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100311098A1 (en) * 2008-01-15 2010-12-09 Universiteit Utrecht Holding B.V. Method for determining the amino acid sequence of peptides
WO2010065322A1 (en) * 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides

Also Published As

Publication number Publication date
AU2020376814A1 (en) 2022-06-02
US20210148922A1 (en) 2021-05-20
MX2022005094A (es) 2022-08-15
KR20220108056A (ko) 2022-08-02
JP2023501932A (ja) 2023-01-20
BR112022007937A2 (pt) 2022-08-30
WO2021086918A1 (en) 2021-05-06
CA3159362A1 (en) 2021-05-06
EP4051806A1 (en) 2022-09-07

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