CN114929862A - 用于制备表达嵌合抗原受体的t细胞的生产方法 - Google Patents

用于制备表达嵌合抗原受体的t细胞的生产方法 Download PDF

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CN114929862A
CN114929862A CN202080087663.5A CN202080087663A CN114929862A CN 114929862 A CN114929862 A CN 114929862A CN 202080087663 A CN202080087663 A CN 202080087663A CN 114929862 A CN114929862 A CN 114929862A
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cells
cell
car
population
leu
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于慧
D.卡莱奇迪斯
S.谭
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CRISPR Therapeutics AG
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CN202080087663.5A 2019-11-13 2020-11-13 用于制备表达嵌合抗原受体的t细胞的生产方法 Pending CN114929862A (zh)

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US201962934991P 2019-11-13 2019-11-13
US62/934,991 2019-11-13
PCT/IB2020/060723 WO2021095013A1 (en) 2019-11-13 2020-11-13 Manufacturing process for making t cells expressing chimeric antigen receptors

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US (1) US20210139850A1 (https=)
EP (1) EP4058560A1 (https=)
JP (1) JP2023501580A (https=)
KR (1) KR20220097984A (https=)
CN (1) CN114929862A (https=)
AR (1) AR120469A1 (https=)
AU (1) AU2020382219A1 (https=)
BR (1) BR112022009152A2 (https=)
CA (1) CA3158122A1 (https=)
CO (1) CO2022006008A2 (https=)
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Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116322716A (zh) 2020-09-23 2023-06-23 克里斯珀医疗股份公司 Regnase-1和/或TGFBRII被破坏的基因工程化T细胞具有改善的功能性和持久性
TW202328444A (zh) * 2021-07-26 2023-07-16 瑞士商Ｃｒｉｓｐｒ治療公司 用於製造基因工程化ｃａｒ－ｔ細胞之方法
WO2023084399A1 (en) * 2021-11-09 2023-05-19 Crispr Therapeutics Ag Genetically engineered immune cells expressing masked chimeric antigen receptors specific to protein tyrosine kinase 7
US20230303713A1 (en) * 2022-03-23 2023-09-28 Crispr Therapeutics Ag Anti-cd19 car-t cells with multiple gene edits and therapeutic uses thereof
WO2025196582A1 (en) * 2024-03-18 2025-09-25 Immuneel Therapeutics Private Limited A method for preparing chimeric antigen receptor (car) expressing cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016069283A1 (en) * 2014-10-31 2016-05-06 The Trustees Of The University Of Pennsylvania Altering gene expression in cart cells and uses thereof
WO2018115887A1 (en) * 2016-12-21 2018-06-28 Ucl Business Plc Therapeutic cells
CN109790517A (zh) * 2016-04-15 2019-05-21 纪念斯隆-凯特林癌症中心 转基因t细胞和嵌合抗原受体t细胞组合物和相关方法
WO2019097305A2 (en) * 2017-05-12 2019-05-23 Crispr Therapeutics Ag Materials and methods for engineering cells and uses thereof in immuno-oncology

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2013266968B2 (en) * 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
CN113355357B (zh) * 2012-12-12 2024-12-03 布罗德研究所有限公司 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
ES2836743T3 (es) * 2014-06-02 2021-06-28 Us Health Receptores de antígeno quiméricos que seleccionan como diana CD-19
GB201418965D0 (https=) * 2014-10-24 2014-12-10 Ospedale San Raffaele And Fond Telethon
CN107250373A (zh) * 2015-01-12 2017-10-13 麻省理工学院 通过微流体递送实现的基因编辑
US20190136230A1 (en) * 2016-05-06 2019-05-09 Juno Therapeutics, Inc. Genetically engineered cells and methods of making the same
CN109790519A (zh) * 2016-06-22 2019-05-21 塞尔丽思股份公司 细胞靶向的药物活性物质或标记物递送体系
DE102016211884A1 (de) 2016-06-30 2018-01-04 Zf Friedrichshafen Ag Getriebe für ein Kraftfahrzeug, sowie Antriebsstrang für ein Kraftfahrzeug
WO2018157072A1 (en) * 2017-02-27 2018-08-30 Life Technologies Corporation Expansion of populations of t cells by the use of modified serum free media
CN107392652A (zh) 2017-06-30 2017-11-24 广州市中崎商业机器股份有限公司 基于收银纸背面进行针对性广告发布的系统及方法
AU2018313167A1 (en) * 2017-08-08 2020-02-27 Sangamo Therapeutics, Inc. Chimeric antigen receptor mediated cell targeting
CN108192927A (zh) * 2017-12-29 2018-06-22 上海海洋大学 一种瓯江彩鲤的基因编辑与过表达操作方法
BR112020016176A2 (pt) * 2018-02-09 2022-02-22 Immatics Us Inc Métodos de transdução de uma célula t, célula t transduzida geneticamente, composição farmacêutica, métodos de preparação de uma população de células t e uso da célula t
MX2020012028A (es) 2018-05-11 2021-03-29 Crispr Therapeutics Ag Metodos y composiciones para tratar el cancer.

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016069283A1 (en) * 2014-10-31 2016-05-06 The Trustees Of The University Of Pennsylvania Altering gene expression in cart cells and uses thereof
CN109790517A (zh) * 2016-04-15 2019-05-21 纪念斯隆-凯特林癌症中心 转基因t细胞和嵌合抗原受体t细胞组合物和相关方法
WO2018115887A1 (en) * 2016-12-21 2018-06-28 Ucl Business Plc Therapeutic cells
WO2019097305A2 (en) * 2017-05-12 2019-05-23 Crispr Therapeutics Ag Materials and methods for engineering cells and uses thereof in immuno-oncology

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JIANGTAO REN ET AL.: "Multiplex Genome Editing to Generate Universal CAR T Cells Resistant to PD1 Inhibition", CLIN CANCER RES., vol. 23, no. 9, pages 2255 - 2266, XP055565027, DOI: 10.1158/1078-0432.CCR-16-1300 *

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