CN114921328A - Liquid high-purification separation device for preparing biological enzyme and preparation method thereof - Google Patents
Liquid high-purification separation device for preparing biological enzyme and preparation method thereof Download PDFInfo
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- CN114921328A CN114921328A CN202210775837.8A CN202210775837A CN114921328A CN 114921328 A CN114921328 A CN 114921328A CN 202210775837 A CN202210775837 A CN 202210775837A CN 114921328 A CN114921328 A CN 114921328A
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Abstract
The invention discloses a high-purification and separation device for liquid for preparing biological enzyme and a preparation method thereof, relating to the technical field of biological enzyme, and comprising an outer cylinder and a centrifugal mechanism arranged in the outer cylinder, wherein the bottom of the outer cylinder is provided with a purified liquid outlet pipe; this centrifugal mechanism includes: and the centrifugal cylinder is assembled inside the outer cylinder body and can rotate in the circumferential direction under the driving of the first driving part. When the first electromagnetic valve is opened, liquid to be filtered is led into the annular centrifugal cavity for filtering under the centrifugal action of the centrifugal cylinder and the positive pressure action of the separation cavity, and when the first electromagnetic valve is closed, external gas reversely passes through the filter holes of the microfiltration membrane component under the negative pressure action generated inside the separation cavity by the movable part, so that impurities blocked on the microfiltration membrane component are removed, the adhesion of impurities to the filter holes is avoided, the continuous working time of the microfiltration membrane is prolonged, the replacement frequency is reduced, and the long-term stable and effective work of equipment is ensured.
Description
Technical Field
The invention relates to the technical field of biological enzyme, in particular to a high-purification separation device for liquid for preparing biological enzyme and a preparation method thereof.
Background
When the biological enzyme is prepared, the biological enzyme extracted from the culture solution needs to be subjected to microfiltration purification so as to obtain the biological enzyme with higher purity, the biological enzyme is mainly filtered through a microfiltration membrane, the microfiltration membrane generally refers to a filtration membrane with the filtration pore size of 0.1-1 micron, because the filtration pore of the microfiltration membrane is very small, the micro-filtration membrane is easy to be blocked in the continuous micro-filtration purification process, the flow is reduced, the traditional solution is to replace the micro-filtration membrane, for example, Chinese patent (No. CN 206872822U) discloses a device for microfiltration, purification and separation of biological enzyme liquid, which designs a disassembly structure of a microfiltration membrane, so that the device is convenient to replace, saves the downtime, is a cumbersome method for replacing blockage, needs cleaning equipment for reuse after each replacement, still consumes a great deal of time, how to reduce replacement operation and reduce the blockage of the micro-filtration membrane and ensure the long-term stable operation of the equipment is a problem which needs to be solved urgently.
Disclosure of Invention
The invention aims to: the liquid high-purification separation device for preparing the biological enzyme and the preparation method thereof are provided to solve the problems that the micro-filtration membrane provided in the background technology generally refers to a filtration membrane with the filtration pore diameter of 0.1-1 micron, and the micro-filtration membrane is easy to block and the flow rate is reduced in the continuous micro-filtration purification process because the filtration pore of the micro-filtration membrane is very small.
In order to realize the purpose, the invention adopts the following technical scheme:
the invention provides a high-purification and separation device for liquid for preparing biological enzyme, which is characterized by comprising an outer cylinder and a centrifugal mechanism arranged in the outer cylinder, wherein the bottom of the outer cylinder is provided with a purified liquid outlet pipe; this centrifugal mechanism includes: the centrifugal cylinder is assembled inside the outer cylinder body and can rotate in the circumferential direction under the driving of the first driving part, and a pressure cavity and a separation cavity which are communicated with each other are formed inside the centrifugal cylinder from top to bottom; the moving part is movably and hermetically matched with the pressure cavity and can bidirectionally and reciprocally slide along the axial direction of the pressure cavity under the driving of the second driving part; the clamping part comprises an upper clamping ring and a lower clamping ring which are respectively arranged on the outer side wall of the centrifugal cylinder, and a microfiltration membrane assembly, the clamping part can be detachably arranged between the upper clamping ring and the lower clamping ring, and an annular centrifugal cavity is formed by the upper clamping ring, the lower clamping ring, the microfiltration membrane assembly and the centrifugal cylinder in a surrounding way; liquid discharge pipes are uniformly arranged at the bottom end between the annular centrifugal cavity and the separation cavity along the circumferential direction, and air inlet pipes are uniformly arranged at the top end between the annular centrifugal cavity and the separation cavity along the circumferential direction; the liquid discharge pipe is provided with a first electromagnetic valve, and the air inlet pipe is provided with a one-way valve; the centrifugal mechanism further comprises an upper switch and a lower switch which are respectively arranged at the upper end and the lower end of the pressure cavity, the movable piece is provided with an upper terminal position for opening the upper switch and a lower terminal position for opening the lower switch, the first electromagnetic valve is opened when the movable piece is located at the upper terminal position, and the first electromagnetic valve is closed when the movable piece is located at the lower terminal position.
Furthermore, a liquid inlet pipe is coaxially arranged at the top of the centrifugal cylinder, penetrates through the pressure cavity, extends to a liquid outlet end at the top end inside the separation cavity and is provided with a top wall liquid inlet end penetrating through the outer cylinder body, the liquid inlet end is connected with a first external connecting pipe through a rotary joint, and the external liquid inlet pipe is in sealed rotary connection with the top wall of the outer cylinder body; a liquid outlet pipe communicated with the inside of the separation cavity is coaxially arranged at the bottom of the centrifugal cylinder, one end of the liquid outlet pipe, far away from the centrifugal cylinder, is connected with a second external connecting pipe through a rotary joint, a fixed ring seat is coaxially arranged on the outer side of the liquid outlet pipe, supporting columns are uniformly arranged at the bottom of the fixed ring seat, one ends, far away from the fixed ring shape, of the supporting columns extend to be fixedly connected with the bottom end inside the outer cylinder body, and the liquid outlet pipe is rotatably connected with the fixed ring seat; and the liquid inlet pipe and the liquid outlet pipe are both provided with second electromagnetic valves.
Further, the first driving part comprises a first gear assembled on the outer side wall of the centrifugal cylinder and a driving motor arranged on one side of the first gear; the output end of the driving motor is fixedly connected with a second gear, and the second gear is meshed with the first gear.
Further, the second driving part includes: the annular track is coaxial with the centrifugal cylinder and is assembled at the top end of the inner part of the outer cylinder body, and ramps which are raised towards one side of the centrifugal cylinder are symmetrically arranged at two sides of the annular track; the centrifugal cylinder comprises a centrifugal cylinder top wall, fixing columns and a ring-shaped track, wherein the centrifugal cylinder top wall is provided with a centrifugal cylinder top wall, the centrifugal cylinder top wall is provided with a circular track, and the centrifugal cylinder top wall is in contact with the circular track; and the return spring is assembled between the movable part outside the fixed column and the top wall of the centrifugal cylinder, and provides damping when the movable part moves upwards.
Furthermore, the centrifugal cylinder comprises a cylindrical upper shell corresponding to the pressure cavity and a truncated cone-shaped lower shell corresponding to the separation cavity, and the cross section of the truncated cone-shaped lower shell is continuously reduced along the downward direction of the axial direction; go up the joint ring with lower joint ring is fixed respectively at the lateral wall of the upper and lower extreme of round platform shape inferior valve, the microfiltration membrane subassembly includes the last ring body with last joint ring looks adaptation, with the lower ring body of joint ring looks adaptation down to and set up the round platform shape microfiltration membrane between last ring body and lower ring body.
Furthermore, a partition plate is arranged inside the separation cavity, the partition plate comprises an annular plate fixed on the side wall of the separation cavity below the air inlet pipe and an isolation cylinder which axially extends downwards along the periphery of the inner side of the annular plate and is fixedly connected with the bottom of the separation cavity, and the cross section of the isolation cylinder is continuously increased along the axial downward direction; the baffle plate enables the separation cavity to form a circular table cavity and an isolation cavity, the liquid discharge pipe is located in the isolation cavity, and two ends of the liquid discharge pipe are respectively connected with the circular table cavity and the annular centrifugal cavity.
Furthermore, the inside of the annular centrifugal cavity is uniformly provided with fixing strips fixed on the side wall of the truncated cone-shaped lower shell along the circumferential direction.
The invention also provides a preparation method of the liquid high-purification separation device for preparing the biological enzyme, which comprises the following steps of: and introducing the crude enzyme solution into a separation cavity of a liquid high-purification separation device for preparing the biological enzyme, starting a second driving part to enable a moving part to reciprocate up and down in the separation cavity, intermittently generating positive pressure and negative pressure in the separation cavity, synchronously starting a first driving part to enable a centrifugal cylinder to rotate, and intermittently enabling the crude enzyme solution to enter an annular centrifugal cavity from a liquid discharge pipe for centrifugal separation by utilizing centrifugal force and positive pressure to obtain a refined enzyme solution.
Compared with the prior art, the above one or more technical schemes have the following beneficial effects:
when the centrifugal cylinder of the invention rotates in the circumferential direction, the moving part positioned in the pressure cavity also reciprocates up and down synchronously, when the moving part moves downwards, the first electromagnetic valve of the liquid discharge pipe is opened, the liquid to be filtered in the separation cavity can be guided into the annular centrifugal cavity for filtering under the action of the centrifugal force generated by the rotation of the centrifugal cylinder, meanwhile, the moving part can make the separation cavity generate positive pressure to act on the liquid to be measured, thereby accelerating the filtration efficiency of the liquid to be measured, when the moving part moves upwards, the first electromagnetic valve of the liquid discharge pipe is closed, the moving part can enable the separation cavity to generate negative pressure so as to enable the outside air to reversely pass through the microfiltration membrane component, enter the annular centrifugal cavity and then enter the separation cavity, the impurities blocked on the microfiltration membrane component are removed, so that the impurities are prevented from being attached to the filter holes, the continuous working time of the microfiltration membrane is prolonged, the replacement frequency is reduced, and the long-term stable and effective work of the equipment is ensured.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
In the drawings:
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic structural view of the present invention with a portion of the outer cylinder removed;
FIG. 3 is a schematic cross-sectional view of the present invention;
FIG. 4 is a schematic view of the internal structure of the centrifugal cylinder according to the present invention;
FIG. 5 is a schematic view of a portion of the structure of FIG. 4;
FIG. 6 is a schematic view of the centrifugal cylinder of the present invention after removing the microfiltration membrane module.
In the figure:
1. an outer cylinder; 2. a centrifugal cylinder; 3. a pressure chamber; 4. a separation chamber; 5. a movable member; 6. an upper clamping ring; 7. an upper clamping ring; 8. a microfiltration membrane component; 9. an annular centrifugal chamber; 10. a liquid discharge pipe; 11. an air inlet pipe; 12. a first solenoid valve; 13. a one-way valve; 14. an upper switch; 15. a lower switch; 16. a liquid inlet pipe; 17. a first external pipe; 18. a liquid outlet pipe; 19. a stationary ring seat; 20. a support pillar; 21. a second solenoid valve; 22. a first gear; 23. a drive motor; 24. a second gear; 25. an annular track; 26. a ramp; 27. fixing the column; 28. rolling a ball; 29. a return spring; 30. a cylindrical upper shell; 31. a truncated cone-shaped lower shell; 32. a partition plate; 33. a circular table cavity; 34. an isolation chamber; 161. a liquid outlet end; 162. a liquid inlet end; 81. an upper ring body; 82. a lower ring body; 83. a truncated cone-shaped microfiltration membrane; 321. an annular plate; 322. an isolation cylinder.
Detailed Description
In order to make the technical solutions of the present application better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only some embodiments of the present application, and not all embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making any creative effort shall fall within the protection scope of the present application.
It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It should be understood that the data so used may be interchanged under appropriate circumstances in order to facilitate the description of the embodiments of the application herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
In this application, the terms "upper", "lower", "left", "right", "front", "rear", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings. These terms are used primarily to better describe the present application and its embodiments, and are not used to limit the indicated devices, elements or components to a particular orientation or to be constructed and operated in a particular orientation.
Moreover, some of the above terms may be used to indicate other meanings besides the orientation or positional relationship, for example, the term "on" may also be used to indicate some kind of attachment or connection relationship in some cases. The specific meaning of these terms in this application will be understood by those of ordinary skill in the art as the case may be.
Furthermore, the terms "mounted," "disposed," "provided," "connected," and "coupled" are to be construed broadly. For example, it may be a fixed connection, a removable connection, or a unitary construction; can be a mechanical connection, or an electrical connection; may be directly connected, or indirectly connected through intervening media, or may be in internal communication between two devices, elements or components. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.
Referring to fig. 1 to 6, the present invention provides a high purification and separation apparatus for liquid used in preparation of biological enzyme, including an outer cylinder 1 and a centrifugal mechanism disposed inside the outer cylinder 1, wherein a purified liquid outlet pipe is disposed at the bottom of the outer cylinder 1; this centrifugal mechanism includes: the centrifugal cylinder 2 is assembled inside the outer cylinder body 1 and can rotate in the circumferential direction under the driving of the first driving part, and a pressure cavity 3 and a separation cavity 4 which are communicated with each other are formed inside the centrifugal cylinder 2 from top to bottom respectively; the movable piece 5 is movably matched with the pressure cavity 3 in a sealing way and can bidirectionally slide in a reciprocating way along the axial direction of the pressure cavity 3 under the driving of the second driving part; the clamping part comprises an upper clamping ring 6 and an upper clamping ring 7 which are respectively arranged on the outer side wall of the centrifugal cylinder 2, and a microfiltration membrane component 8, and is detachably arranged between the upper clamping ring 6 and the upper clamping ring 7, and an annular centrifugal cavity 9 is formed by encircling the upper clamping ring 6, the upper clamping ring 7, the microfiltration membrane component 8 and the centrifugal cylinder 2; liquid discharge pipes 10 are uniformly arranged at the bottom end between the annular centrifugal cavity 9 and the separation cavity 4 along the circumferential direction, and air inlet pipes 11 are uniformly arranged at the top end between the annular centrifugal cavity 9 and the separation cavity 4 along the circumferential direction; a first electromagnetic valve 12 is arranged on the liquid discharge pipe 10, and a one-way valve 13 is arranged on the air inlet pipe 11;
the centrifugal mechanism further comprises an upper switch 14 and a lower switch 15 respectively arranged at the upper end and the lower end of the pressure chamber 3, the movable member 5 has an upper terminal position for opening the upper switch 14 and a lower terminal position for opening the lower switch 15, the first electromagnetic valve 12 is opened when the movable member 5 is located at the upper terminal position, and the first electromagnetic valve 12 is closed when the movable member 5 is located at the lower terminal position.
When the centrifugal cylinder works, the centrifugal cylinder 2 is driven by the first driving part to rotate in the circumferential direction, and the moving part 5 is driven by the second driving part to bidirectionally slide in a reciprocating manner along the axial direction of the pressure cavity 3; when the moving element 5 moves from the upper terminal position to the lower terminal position, firstly, the moving element 5 can trigger the upper switch 14 at the upper terminal position, so that the first electromagnetic valve 12 on the liquid discharge pipe 10 is opened, and at this time, under the action of centrifugal force generated by circumferential rotation of the centrifugal cylinder 2, the liquid to be filtered in the separation chamber 4 enters the annular centrifugal chamber 9 through the inside of the liquid discharge pipe 10, and under the action of the centrifugal force, the liquid to be filtered enters the inside of the outer cylinder 1 after being filtered by the microfiltration membrane component, meanwhile, in the process of moving the moving element 5 from the upper terminal position to the lower terminal position, positive pressure is generated inside the separation chamber 4 (the reason is that the liquid to be filtered is located at the bottom of the separation chamber 4, which is equivalent to sealing inside the separation chamber 4), and the generated positive pressure can accelerate the liquid to be filtered to move to the annular centrifugal chamber 9 and act on the microfiltration membrane component, the filtering efficiency of the liquid to be detected is accelerated; when the moving member 5 moves from the lower terminal position to the upper terminal position, the moving member 5 can trigger the lower switch 15 at the lower terminal position, so that the first electromagnetic valve 12 on the liquid discharge pipe 10 is closed, at this time, the inside of the separation chamber 4 is blocked, the liquid to be measured cannot enter the annular centrifugal chamber 9, and at the same time, during the moving process of the moving member 5 from the lower terminal position to the upper terminal position, negative pressure is generated inside the separation chamber 4, the generated negative pressure can open the one-way valve 13, because the centrifugal cylinder 2 rotates at this time, the liquid inside the annular centrifugal chamber 9 is still separated, and the annular centrifugal chamber 9 has no liquid supplement, therefore, the inside of the annular centrifugal chamber 9 is in an empty state, the outside gas can enter the annular centrifugal chamber 9 through the microfiltration membrane assembly, and then enters the separation chamber 4 through the air inlet pipe 11, and the gas can reversely pass through the filtration pores of the microfiltration membrane assembly, and then will block up the impurity of microfiltration membrane module and get rid of, avoid debris to adhere to at the filtration pore, prolong the continuous operation time of microfiltration membrane, reduce the change frequency, ensure the long-term stable effectual work of equipment.
In this embodiment, the top of the centrifuge bowl 2 is coaxially provided with a liquid inlet pipe 16, and the liquid inlet pipe 16 has a liquid outlet end 161 penetrating through the pressure chamber 3 and extending to the top end inside the separation chamber 4 and a liquid inlet end 162 penetrating through the top wall of the outer bowl 1, the liquid inlet end 162 is connected with a first external pipe 17 through a rotary joint, and the liquid inlet pipe 16 is connected with the top wall of the outer bowl 1 in a sealing and rotating manner; a liquid outlet pipe 18 communicated with the inside of the separation cavity 4 is coaxially arranged at the bottom of the centrifugal cylinder 2, one end, far away from the centrifugal cylinder 2, of the liquid outlet pipe 18 is connected with a second external connecting pipe 171 through a rotary joint, a fixed ring seat 19 is coaxially arranged on the outer side of the liquid outlet pipe 18, supporting columns 20 are uniformly arranged at the bottom of the fixed ring seat 19, one end, far away from the fixed ring shape, of each supporting column 20 extends to be fixedly connected with the bottom end inside the outer cylinder body 1, and the liquid outlet pipe 18 is rotatably connected with the fixed ring seat 19; the liquid inlet pipe 16 and the liquid outlet pipe 18 are both provided with a second electromagnetic valve 21. Through the structure, the stationarity of centrifuge bowl 2 in the rotation process has been ensured, and the accessible is first external to take over 17 can be with waiting to filter in the liquid guide-in separation chamber 4 (need open the second solenoid valve 21 on the feed liquor pipe 16 when leading-in, when waiting to filter the leading-in completion of liquid, close the second solenoid valve 21 on the feed liquor pipe 16), and can be with remaining residual liquid discharge (need open the second solenoid valve 21 on the drain pipe 18 when discharging) in separation chamber 4 inside through the external pipe of second (the residual liquid discharges the completion after, close the second solenoid valve 21 on the drain pipe 18).
In this embodiment, the first driving part comprises a first gear 22 assembled on the outer side wall of the centrifugal cylinder 2, and a driving motor 23 arranged on one side of the first gear 22; the output end of the driving motor 23 is fixedly connected with a second gear 24, and the second gear 24 is meshed with the first gear 22. Through the structure, the output end of the driving motor 23 drives the second gear 24 to rotate, and then the first gear 22 meshed with the second gear 24 rotates, so that the centrifugal cylinder 2 rotates axially.
In this embodiment, the second driving part includes: an annular rail 25 which is coaxial with the centrifugal cylinder 2 and is assembled at the top end of the inner part of the outer cylinder body 1, wherein ramps 26 which are raised towards one side of the centrifugal cylinder 2 are symmetrically arranged at two sides of the annular rail 25; the fixed posts 27 are symmetrically fixed on two sides of the top of the moving part 5, one end of each fixed post 27, which is far away from the moving part 5, penetrates through the top wall of the centrifugal cylinder 2 and is provided with a ball groove at the end part, a rolling ball 28 is clamped inside the ball groove, and the rolling ball 28 is abutted against the annular track 25; and a return spring 29, which is arranged between the movable member 5 outside the fixed column 27 and the top wall of the centrifugal cylinder 2 and provides damping when the movable member 5 moves upwards. Through the structure, the centrifugal cylinder 2 can synchronously drive the fixed column 27 to rotate when rotating in the circumferential direction, the arranged return spring 29 can enable the rolling ball 28 arranged at the end part of the fixed column 27 to always abut against the annular track, when the rolling ball 28 of the fixed column 27 passes through the lug of the annular track, the fixed column 27 can slide downwards after sliding upwards along the ramp 26, so that the moving part 5 finishes one-time up-and-down fluctuation inside the pressure cavity 3, and positive pressure and negative pressure are formed inside the centrifugal cylinder 2.
In this embodiment, the centrifugal cylinder 2 comprises a cylindrical upper shell 30 corresponding to the pressure chamber 3 and a truncated cone-shaped lower shell 31 corresponding to the separation chamber 4, and the cross section of the truncated cone-shaped lower shell 31 along the axial downward direction is continuously reduced; the upper clamping ring 6 and the upper clamping ring 7 are respectively fixed on the outer side walls of the upper end and the lower end of the truncated cone-shaped lower shell 31, and the microfiltration membrane component 8 comprises an upper ring body 81 matched with the upper clamping ring 6, a lower ring body 82 matched with the upper clamping ring 7, and a truncated cone-shaped microfiltration membrane 83 arranged between the upper ring body 81 and the lower ring body 82. Through the structure, wherein go up ring 81 and last joint ring 6 threaded connection, lower ring 82 and last joint ring 7 threaded connection, after waiting to filter liquid and getting into cyclic annular centrifugal chamber 9, can slide up until being full of cyclic annular centrifugal chamber 9 along circular truncated cone shape micro-filtration membrane 83 under the effect of centrifugal force, wait to filter liquid and the contact of whole circular truncated cone shape micro-filtration membrane 83 inner wall, accomplish and filter, (no matter how much of the height of the inside liquid level of separation chamber 4, liquid in the cyclic annular centrifugal chamber 9 all can contact with whole circular truncated cone shape micro-filtration membrane 83 inner wall, accomplish the filtration of big flux), purification separation efficiency has been improved.
In this embodiment, a partition plate 32 is disposed inside the separation chamber 4, the partition plate 32 includes an annular plate 321 fixed on the sidewall of the separation chamber 4 below the air inlet pipe 11, and an isolation cylinder 322 extending axially downward along the inner periphery of the annular plate 321 and fixedly connected to the bottom of the separation chamber 4, and the cross section of the isolation cylinder 322 increases continuously along the axial downward direction; partition 32 is used to form a circular table cavity 33 and an isolation cavity 34 in separation cavity 4, drain pipe 10 is located in isolation cavity 34, and two ends of drain pipe 10 are respectively connected with circular table cavity 33 and annular centrifugal cavity 9. Through the structure, the arrangement of the partition plate 32 enables the liquid to be filtered to be positioned in the circular table cavity 33, so that most of the centrifugal force generated when the centrifugal cylinder 2 rotates acts on the bottom of the circular table cavity 33, the speed of the liquid to be filtered passing through the liquid discharge pipe 10 is accelerated, and the liquid in the circular table cavity 33 is prevented from moving upwards due to the centrifugal force.
In this embodiment, the annular centrifugal cavity 9 is uniformly provided with fixing strips fixed on the sidewall of the truncated cone-shaped lower shell 31 along the circumferential direction. Through the structure, the centrifugal force is improved, and the filtering efficiency of liquid in the annular centrifugal cavity 9 is facilitated.
Example one
The preparation method of the biological enzyme applied to the liquid high-purification separation device for preparing the biological enzyme comprises the following steps:
s1 pretreatment: cleaning fruits and pulping;
s2 enzymolysis: adding lignin degrading enzyme into the fruit pulp obtained in the step S1, adjusting the pH value to 5, stirring, carrying out solid-liquid separation, and collecting supernatant;
s3 purification and separation: introducing the collected supernatant into a separation cavity 4 of a high-purification separation device for biological enzyme preparation liquid, starting a second driving part to enable a moving part 5 to reciprocate up and down in the separation cavity 4, intermittently generating positive pressure and negative pressure in the separation cavity 4, synchronously starting a first driving part to enable a centrifugal cylinder 2 to rotate, and intermittently allowing a crude enzyme solution to enter an annular centrifugal cavity 4 from a liquid discharge pipe 10 for centrifugal separation by utilizing centrifugal force and positive pressure to obtain trapped liquid;
s4 spray drying: and (4) carrying out spray drying on the trapped fluid obtained in the step S3, wherein a freeze-dried product after drying is the biological enzyme.
Example two
The preparation method of the biological enzyme applied to the liquid high-purification separation device for preparing the biological enzyme comprises the following steps:
s1 pretreatment: cleaning with flowing water by spraying to remove impurities and dirt on the surface of a fresh egg, soaking in a chlorine dioxide solution with the chlorine dioxide content of 30mg/L for 20 minutes, blowing off water on the surface of the egg with hot air after soaking, breaking the shell with a egg breaker, separating egg white and egg yolk, sterilizing the egg white, and feeding the egg white into a raw material storage tank;
s2 purification and separation: introducing the collected supernatant into a separation cavity 4 of a liquid high-purification separation device for preparing the biological enzyme, starting a second driving part to enable a moving part 5 to reciprocate up and down in the separation cavity 4, intermittently generating positive pressure and negative pressure in the separation cavity 4, synchronously starting a first driving part to enable a centrifugal cylinder 2 to rotate, and intermittently enabling a crude enzyme solution to enter an annular centrifugal cavity 4 from a liquid discharge pipe 10 for centrifugal separation by utilizing centrifugal force and positive pressure to obtain a filtrate;
s3 concentration: sending the filtered solution into an ion exchange resin chromatographic column for chromatography, wherein during chromatography, a balance solution is an improved phosphate buffer solution PSB with the pH value of 5.0, eluting after chromatography, an elution solution is the improved phosphate buffer solution PSB with the pH value of 5, and the NaCl content in the improved phosphate buffer solution PSB is linearly increased from 0 to 0.5mol/L within 20 minutes by adopting a gradient elution mode; detecting the eluate, collecting qualified eluate with wavelength of 260nm, and filtering with ultrafiltration membrane with cut-off molecular weight of 5000D to obtain cut-off concentrated solution, and removing the permeate;
s3 drying: and (3) carrying out spray drying on the trapped concentrated solution, wherein the air inlet temperature is 110 ℃, the air outlet temperature is 70 ℃, and drying to obtain the finished product of lysozyme.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art.
Claims (8)
1. The high-purification separation device for liquid for preparing the biological enzyme is characterized by comprising an outer cylinder and a centrifugal mechanism arranged in the outer cylinder, wherein a purified liquid outlet pipe is arranged at the bottom of the outer cylinder; this centrifugal mechanism includes: the centrifugal cylinder is assembled inside the outer cylinder body and can rotate in the circumferential direction under the driving of the first driving part, and a pressure cavity and a separation cavity which are communicated with each other are formed inside the centrifugal cylinder from top to bottom; the moving part is movably and hermetically matched with the pressure cavity and can bidirectionally slide in a reciprocating manner along the axial direction of the pressure cavity under the driving of the second driving part; the clamping part comprises an upper clamping ring, a lower clamping ring and a microfiltration membrane assembly, wherein the upper clamping ring and the lower clamping ring are respectively arranged on the outer side wall of the centrifugal cylinder, the microfiltration membrane assembly is detachably arranged between the upper clamping ring and the lower clamping ring, and an annular centrifugal cavity is formed by the upper clamping ring, the lower clamping ring, the microfiltration membrane assembly and the centrifugal cylinder in a surrounding manner; liquid discharge pipes are uniformly arranged at the bottom end between the annular centrifugal cavity and the separation cavity along the circumferential direction, and air inlet pipes are uniformly arranged at the top end between the annular centrifugal cavity and the separation cavity along the circumferential direction; the liquid discharge pipe is provided with a first electromagnetic valve, and the air inlet pipe is provided with a one-way valve; the centrifugal mechanism further comprises an upper switch and a lower switch which are respectively arranged at the upper end and the lower end of the pressure cavity, the movable piece is provided with an upper terminal position for opening the upper switch and a lower terminal position for opening the lower switch, the first electromagnetic valve is opened when the movable piece is located at the upper terminal position, and the first electromagnetic valve is closed when the movable piece is located at the lower terminal position.
2. The device for separating and purifying liquid for preparing biological enzyme according to claim 1, wherein a liquid inlet pipe is coaxially arranged at the top of the centrifugal cylinder, passes through the pressure chamber, extends to a liquid outlet end at the top end inside the separation chamber, and has a liquid inlet end penetrating through the top wall of the outer cylinder body, the liquid inlet end is connected with a first external connecting pipe through a rotary joint, and the external liquid inlet pipe is in sealed and rotary connection with the top wall of the outer cylinder body; a liquid outlet pipe communicated with the inside of the separation cavity is coaxially arranged at the bottom of the centrifugal cylinder, one end of the liquid outlet pipe, far away from the centrifugal cylinder, is connected with a second external connecting pipe through a rotary joint, a fixed ring seat is coaxially arranged on the outer side of the liquid outlet pipe, supporting columns are uniformly arranged at the bottom of the fixed ring seat, one ends, far away from the fixed ring shape, of the supporting columns extend to be fixedly connected with the bottom end inside the outer cylinder body, and the liquid outlet pipe is rotatably connected with the fixed ring seat;
and the liquid inlet pipe and the liquid outlet pipe are both provided with second electromagnetic valves.
3. The apparatus for separating and purifying a liquid for bio-enzyme preparation according to claim 1, wherein said first driving means comprises a first gear installed on the outer sidewall of said centrifuge tube, and a driving motor installed on one side of said first gear; the output end of the driving motor is fixedly connected with a second gear, and the second gear is meshed with the first gear.
4. The apparatus for highly purifying and separating a liquid for use in the preparation of a biological enzyme according to claim 1, wherein the second driving means comprises: the annular track is coaxial with the centrifugal cylinder and is assembled at the top end of the inner part of the outer cylinder body, and ramps which are raised towards one side of the centrifugal cylinder are symmetrically arranged at two sides of the annular track; the centrifugal cylinder comprises a centrifugal cylinder top wall, fixed columns symmetrically fixed on two sides of the top of a movable part, and a ball groove, wherein one end of each fixed column, which is far away from the movable part, penetrates through the top wall of the centrifugal cylinder, and the end part of each fixed column is provided with a ball groove; and the return spring is assembled between the movable part outside the fixed column and the top wall of the centrifugal cylinder, and provides damping when the movable part moves upwards.
5. The apparatus for highly purifying and separating a liquid for use in the preparation of a biological enzyme according to claim 1, wherein the centrifuge bowl comprises a cylindrical upper shell corresponding to the pressure chamber and a truncated cone-shaped lower shell corresponding to the separation chamber, and the truncated cone-shaped lower shell decreases in cross section in a downward direction of the axial direction; the upper clamping ring and the lower clamping ring are respectively fixed on the outer side wall of the upper end and the outer side wall of the lower end of the circular truncated cone-shaped lower shell, and the microfiltration membrane component comprises an upper ring body matched with the upper clamping ring, a lower ring body matched with the lower clamping ring, and a circular truncated cone-shaped microfiltration membrane arranged between the upper ring body and the lower ring body.
6. The high-purification and separation device for liquid used for preparing the biological enzyme according to claim 5, wherein a partition is arranged inside the separation chamber, the partition comprises an annular plate fixed on the side wall of the separation chamber below the air inlet pipe, and a separation cylinder extending axially and downwardly along the inner periphery of the annular plate and fixedly connected with the bottom of the separation chamber, and the cross section of the separation cylinder is increased continuously along the axial downward direction; the baffle plate enables the separation cavity to form a circular table cavity and an isolation cavity, the liquid discharge pipe is located in the isolation cavity, and two ends of the liquid discharge pipe are respectively connected with the circular table cavity and the annular centrifugal cavity.
7. The apparatus for separating and purifying a liquid for use in the preparation of a biological enzyme according to claim 6, wherein the annular centrifugal chamber is uniformly provided with fixing strips fixed on the side wall of the truncated cone-shaped lower casing along the circumferential direction.
8. The method for preparing a liquid high-purification and separation device for preparing a biological enzyme according to claim 1, comprising a purification and separation step, wherein the purification and separation step comprises the following steps: and introducing the crude enzyme solution into a separation cavity of a liquid high-purification separation device for preparing the biological enzyme, starting a second driving part to enable a moving part to reciprocate up and down in the separation cavity, intermittently generating positive pressure and negative pressure in the separation cavity, synchronously starting a first driving part to enable a centrifugal cylinder to rotate, and intermittently enabling the crude enzyme solution to enter an annular centrifugal cavity from a liquid discharge pipe for centrifugal separation by utilizing centrifugal force and positive pressure to obtain a refined enzyme solution.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210775837.8A CN114921328A (en) | 2022-07-03 | 2022-07-03 | Liquid high-purification separation device for preparing biological enzyme and preparation method thereof |
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| CN202210775837.8A CN114921328A (en) | 2022-07-03 | 2022-07-03 | Liquid high-purification separation device for preparing biological enzyme and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117645922A (en) * | 2024-01-30 | 2024-03-05 | 山东省农业科学院畜牧兽医研究所 | Phage lyase separation and purification device of enterobacter source |
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2022
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117645922A (en) * | 2024-01-30 | 2024-03-05 | 山东省农业科学院畜牧兽医研究所 | Phage lyase separation and purification device of enterobacter source |
| CN117645922B (en) * | 2024-01-30 | 2024-04-16 | 山东省农业科学院畜牧兽医研究所 | Phage lyase separation and purification device of enterobacter source |
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Application publication date: 20220819 |