CN114920436A - Method for chemically strengthening bioleaching treatment of anaerobic digestion sludge - Google Patents
Method for chemically strengthening bioleaching treatment of anaerobic digestion sludge Download PDFInfo
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/12—Treatment of sludge; Devices therefor by de-watering, drying or thickening
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/12—Treatment of sludge; Devices therefor by de-watering, drying or thickening
- C02F11/121—Treatment of sludge; Devices therefor by de-watering, drying or thickening by mechanical de-watering
- C02F11/122—Treatment of sludge; Devices therefor by de-watering, drying or thickening by mechanical de-watering using filter presses
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Abstract
The invention discloses a method for bioleaching sludge by chemically strengthening anaerobic digestion, which comprises the following steps: s1 bioleaching to condition anaerobic sludge: inoculating the penicillium simplicissimum NAU-12 bacterial liquid into an anaerobic digested sludge system, and conditioning anaerobic sludge by adopting a bioleaching method; the classification name of the Penicillium notatum NAU-12 is Penicillium simplicissimum with the preservation number of CGMCC NO. 10990; s2 chemical enhanced bioleaching conditioning: adding Fe 2+ And H 2 O 2 Culturing for a specific time; s3, pressure filtration dehydration: and (5) directly performing filter pressing on the sludge obtained in the step (S2) under a plate-and-frame filter press for solid-liquid separation to obtain sludge cakes with the water content of less than 60%. The method adopts penicillium simplicissimum NAU-12 bacterial liquid to carry out bioleaching on anaerobic digested sludge, combines chemical strengthening, can generate an acid environment and is beneficial to Fenton reactionSimultaneously consuming sludge organic substances to promote the release of combined water, thereby improving the deep dehydration performance of the sludge.
Description
Technical Field
The invention belongs to the technical field of bioleaching, and particularly relates to a method for treating sludge by chemically-enhanced anaerobic digestion bioleaching.
Background
With the acceleration of the urbanization process in China and the improvement of the standard of living of the people's economy, the number of urban sewage treatment plants is increased sharply, the sewage treatment capacity is also increased greatly, and finally, a large amount of byproducts (namely sludge) are generated in the sewage treatment. The high water content of the sludge leads to the huge volume of the sludge to be treated, and the subsequent treatment and recycling efficiency of the sludge are greatly restricted. Sludge dewatering is the most effective way to realize the volume reduction of sludge in the sludge treatment link.
The bioleaching technology is a new sludge deep dehydration technology which is newly emerged in recent years, sludge treatment process completely utilizes special bioleaching microorganisms to carry out dehydration performance modification on sludge under aeration conditions, and the sludge is directly subjected to pressure filtration by a plate-and-frame filter press until the water content of a sludge cake is lower than 60 percent, so that the bioleaching technology has the advantages of low operation cost, sludge organic matter and heat value preservation, no increase of the dry matter amount of the dehydrated sludge, complete adoption of the microorganisms, no addition of any flocculating agent or coagulant aid, and complete removal of sludge stink and pathogenic bacteria, and is an environment-friendly sludge treatment technology. Through retrieval, Chinese patent application No. 201410119833.X, the application publication date of 2014 7 and 23, discloses a novel method for quickly conditioning deep dehydration by a sludge bioleaching method, which comprises the steps of firstly carrying out bioleaching conditioning on sludge, changing bound water in the sludge into free water which is easy to remove through the microbial substitution effect, biological oxidation and biological acidification, and simultaneously dissolving heavy metals in the sludge into a water phase and killing pathogens in the sludge. And then collecting the sludge after bioleaching in a homogenizing pool, and directly conveying the sludge to a diaphragm box type filter press with common pressure through a pump for dehydration, wherein the water content of a dehydrated sludge cake is lower than 60%. Although bioleaching technology has obvious advantages in deep dewatering of sludge, it requires a long reaction time, limiting its large-scale engineering application.
Therefore, the invention adopts a new method for chemically strengthening bioleaching treatment of anaerobic digestion sludge to shorten bioleaching conditioning time and realize the aim of deep dehydration of the anaerobic digestion sludge.
Disclosure of Invention
1. Problems to be solved
The invention aims to overcome the defect of long bioleaching reaction period in the prior art, and provides a method for chemically strengthening bioleaching treatment of anaerobic digestion sludge, which shortens the bioleaching reaction period and realizes deep dehydration of the anaerobic digestion sludge.
2. Technical scheme
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a method for chemically strengthening bioleaching treatment of anaerobic digestion sludge comprises the following steps:
s1 bioleaching to condition anaerobic sludge: inoculating the penicillium simplicissimum NAU-12 bacterial liquid into an anaerobic digested sludge system, and conditioning anaerobic sludge by adopting a bioleaching method; the Penicillium notatum NAU-12 is classified and named Penicillium simplicissimum with the preservation number of CGMCC NO. 10990; is preserved in the China general microbiological culture Collection center;
s2 chemical enhanced bioleaching conditioning: adding Fe 2+ And H 2 O 2 Culturing for a specific time;
s3, filter pressing dehydration: and (5) directly performing filter pressing on the sludge obtained in the step (S2) under a plate-and-frame filter press for solid-liquid separation to obtain sludge cakes with the water content of less than 60%.
Preferably, the bacterial cell number of the bacterial strain of Penicillium simplicissimum NAU-12 used in the step S1 isAn amount of 10 7 ～10 8 The inoculation volume is 1 to 20 percent of the anaerobic digestion sludge per mL.
Preferably, the pH of the system after the culture in step S2 is reduced to 3.0 or less.
Preferably, the method for culturing the penicillium simplicissimum NAU-12 bacterial liquid in the step S1 comprises the following steps: inoculating the penicillium simplicissimum NAU-12 separated from the sludge into a martin solid culture medium, and then placing the solid culture medium into a reciprocating shaking table at 28 ℃ and 180-200 rpm for vibration propagation culture until the number of the bacterial cells reaches 10 7 ～10 8 One cell per mL, stored at 4 ℃ for use.
Preferably, the preparation method of the martin solid medium comprises the following steps: dissolving 1.0g of monopotassium phosphate, 0.5g of magnesium sulfate heptahydrate, 5.0g of peptone and 10.0g of glucose in sequence, adding distilled water to supplement the volume to 1000mL, then adding 3.3mL of 1% Bengal solution, adding 15-20 g of agar, and sterilizing for 30min by high-pressure steam at 115 ℃; before use, 0.3mL of streptomycin solution at 1% was added to 100mL of the medium.
Preferably, the bioleaching of the anaerobic sludge in the step S1 is carried out for 1-2 days.
Preferably, the step (Fe in S2) 2+ And H 2 O 2 The mass ratio is 2: 1.
preferably, the time for the chemically enhanced bioleaching conditioning in the step S2 is 1-2 h.
Preferably, step S1 is specifically: inoculating the penicillium simplicissimum NAU-12 bacterial liquid into an anaerobic digestion sludge system, culturing in a constant-temperature shaking table at 28 ℃ and 180r/min for 1d, supplementing water lost every day by a weighing and water supplementing method, taking a sludge sample after culturing, and measuring the pH value of the sludge.
Preferably, the step S2 is specifically: after the sludge is treated by bioleaching for 1d, Fe is added 2+ And H 2 O 2 In which Fe 2+ And H 2 O 2 The mass ratio is 2: 1, placing the mixture in a shaking table with constant temperature of 28 ℃ and 180r/min for culturing for 1-2 h, and taking a sludge sample to measure the pH of the sludge.
3. Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
(1) compared with the prior art, the method has the advantages that on one hand, the introduction of the penicillium simplicissimum NAU-12 bacterial liquid can generate small molecular organic acid, and an acidic environment is formed to facilitate Fenton reaction; on the other hand, the penicillium simplicissimum NAU-12 can consume organic substances (such as decomposed EPS) in the sludge and is beneficial to the release of bound water in the sludge;
(2) aiming at anaerobic digestion sludge, the method adopts the penicillium simplicissimum NAU-12 bacterial liquid for bioleaching, compared with the common thiobacillus ferrooxidans bioleaching, the penicillium simplicissimum NAU-12 bacterial liquid bioleaching does not need to be additionally added with iron ions, and the reaction system avoids the introduction of iron salt to cause secondary pollution (for example, a large amount of iron-containing sludge is caused, and the subsequent treatment cost is increased).
Drawings
FIG. 1 is a process flow diagram of a novel method for chemically enhanced anaerobic digestion sludge bioleaching treatment in the present invention.
Detailed Description
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are conventional products which are not indicated by manufacturers and are commercially available.
As used herein, the term "about" is used to provide the flexibility and inaccuracy associated with a given term, measure or value. The degree of flexibility for a particular variable can be readily determined by one skilled in the art.
Concentrations, amounts, and other numerical data may be presented herein in a range format. It is to be understood that such a range format is used merely for convenience and brevity and should be interpreted flexibly to include not only the numerical values explicitly recited as the limits of the range, but also to include all the individual numerical values or sub-ranges encompassed within that range as if each numerical value and sub-range is explicitly recited. For example, a numerical range of about 1 to about 4.5 should be interpreted to include not only the explicitly recited limit values of 1 to about 4.5, but also include individual numbers (such as 2, 3, 4) and sub-ranges (such as 1 to 3, 2 to 4, etc.). The same principle applies to ranges reciting only one numerical value, such as "less than about 4.5," which should be construed to include all of the aforementioned values and ranges. Further, such interpretation should apply regardless of the breadth of the range or the characteristics being described.
The Penicillium simplicissimum NAU-12 adopted in the invention is classified and named as Penicillium simplicissimum Penicillium Simplicissimum, is preserved in the China general microbiological culture Collection center in 2015 at 6-25 months, and has the preservation number of CGMCC NO. 10990.
The invention is further described with reference to specific examples.
Example 1
Anaerobic digestion sludge bioleaching treatment test of a sewage treatment plant in Taohu New City:
the anaerobic digestion sludge of the sewage treatment plant is treated according to the flow (see attached figure 1). 25L of anaerobic digested sludge of the plant is taken, and the basic properties of the sludge are determined as follows: the pH value is 7.28, the solid content is 2.7 percent, the organic matter is 49.2 percent, and the sludge specific resistance is 1.97 multiplied by 10 13 m/kg。
(1) Culturing penicillium simplicissimum NAU-12 bacterial liquid
Inoculating Penicillium simplicissimum NAU-12(Penicillium simplicisum, preservation number of CGMCC NO.10990) separated from sludge into Martin solid culture medium (Martin solid culture medium is prepared by dissolving potassium dihydrogen phosphate 1.0g, magnesium sulfate heptahydrate 0.5g, peptone 5.0g, and glucose 10.0g sequentially, adding distilled water to make up volume to 1000mL, adding 1% Menglan solution 3.3mL, adding 15g agar, sterilizing with 115 deg.C high pressure steam for 30min, adding 1% streptomycin solution 0.3mL into 100mL culture medium before use, placing in 28 deg.C 180rpm reciprocating shaking table, and performing propagation culture until thallus cell number reaches 10 7 One cell per mL, stored at 4 ℃ for use.
(2) Bioleaching process
Inoculating the penicillium simplicissimum NAU-12 bacterial liquid (10%, v/v) into an anaerobic digestion sludge system, setting 3 groups of parallel experiments for each treatment, respectively culturing in a constant-temperature shaking table at 28 ℃ and 180r/min for 1d, supplementing lost water by a weighing water supplementing method, keeping the water content at about 95%, obtaining sludge after bioleaching modification, and measuring that the pH value of the sludge system is reduced to 3.0.
(3) Chemically enhanced bioleaching conditioning
After the sludge is treated by bioleaching for 1d, 60mg/g of sludge Dry Solids (DS) Fe is added into each system 2+ And 30mg/g DS H 2 O 2 Respectively culturing in shaking table at constant temperature of 28 deg.C and 180r/min for 1 hr, and measuring pH of sludge to 2.8.
(4) Filter pressing dewatering
Conveying the sludge subjected to the chemical strengthening bioleaching conditioning to a plate-and-frame filter press by a pump for direct filter pressing, wherein the feeding time is 30min, the high-pressure water pressing and pressure maintaining are carried out for 30min, the feeding pressure is 0.7MPa, the diaphragm pressing pressure is 1.5MPa, the filter pressing water is colorless and clear at the moment, the pressed mud cake is earthy yellow and odorless, and the water content of the mud cake is determined to be about 56%.
Comparative example 1
Anaerobic digestion sludge bioleaching treatment test of a sewage treatment plant in Taohu New City:
and treating the anaerobic digestion sludge of the sewage treatment plant. 25L of anaerobic digested sludge of the plant is taken, and the basic properties of the sludge are determined as follows: the pH value is 7.28, the solid content is 2.7 percent, the organic matter is 49.2 percent, and the sludge specific resistance is 1.97 multiplied by 10 13 m/kg。
(1) Culturing penicillium notatum NAU-12 bacterial liquid
Inoculating Penicillium simplicissimum NAU-12(Penicillium simplicisum, preservation number of CGMCC NO.10990) separated from sludge into Martin solid culture medium (Martin solid culture medium is prepared by dissolving potassium dihydrogen phosphate 1.0g, magnesium sulfate heptahydrate 0.5g, peptone 5.0g, and glucose 10.0g sequentially, adding distilled water to make up volume to 1000mL, adding 1% Menglan solution 3.3mL, adding 15g agar, sterilizing with 115 deg.C high pressure steam for 30min, adding 1% streptomycin solution 0.3mL into 100mL culture medium before use, placing in 28 deg.C 180rpm reciprocating shaking table, and performing propagation culture until thallus cells are culturedUp to 10 in number 7 One cell per mL, stored at 4 ℃ for use.
(2) Bioleaching process
Inoculating the penicillium simplicissimum NAU-12 bacterial liquid (10%, v/v) into an anaerobic digestion sludge system, setting 3 groups of parallel experiments for each treatment, respectively placing the parallel experiments in a shaking table with constant temperature of 28 ℃ and 180r/min for culturing for 8d, supplementing water lost every day by a weighing and water supplementing method, keeping the water content at about 95%, obtaining sludge after bioleaching modification, and measuring that the pH value of the sludge system is reduced to 2.6.
(3) Filter pressing dewatering
Conveying the sludge conditioned by bioleaching to a plate-and-frame filter press by a pump for direct filter pressing, wherein the feeding time is 30min, the high-pressure water pressing and pressure maintaining are carried out for 30min, the feeding pressure is 0.7MPa, the diaphragm pressing pressure is 1.5MPa, the filter pressing water is colorless and clear, the pressed mud cake is earthy yellow and odorless, and the water content of the mud cake is determined to be about 59%.
Comparative example 2
The anaerobic digestion sludge is treated by bioleaching, the treatment steps are the same as the comparative example 1, and the difference is that in the step (2), only 1d bioleaching conditioning is carried out, the pH value of a sludge system is 5.0, the sludge is conveyed to a plate-and-frame filter press by a pump to be directly pressed and filtered, the feeding time is 30min, the high-pressure water pressing pressure maintaining is 30min, the feeding pressure is 0.7MPa, the diaphragm pressing pressure is 1.5MPa, the pressed mud cake is not shaped, black and odorous, the water content of the pressed mud cake is about 83 percent by measurement, and the pressed and filtered water is muddy water. The biological leaching treatment for 1 day without chemical strengthening is not enough to effectively dehydrate the sludge and takes longer time to deeply dehydrate the sludge.
Comparative example 3
In the same way as in example 1, the thiobacillus ferrooxidans bacterial liquid (10%, v/v) is inoculated into an anaerobic digestion sludge system, 3 groups of parallel experiments are set for each treatment, the parallel experiments are respectively placed in a shaking table with constant temperature of 28 ℃ and 180r/min for culturing for 1d, and the pH value of the sludge system is measured to be reduced to 4.6; then 60mg/g DS Fe is added 2+ And 30mg/g DS H 2 O 2 Respectively culturing in shaking table at constant temperature of 28 deg.C and 180r/min for 1 hr, and measuring pH of sludge to be 4.1. Conveying the sludge after the chemical strengthening bioleaching conditioning to a plate frame by a pump for filter pressingThe machine is used for direct filter pressing, the feeding time is 30min, the pressure is maintained for 30min by high-pressure water pressing, the feeding pressure is 0.7MPa, the diaphragm pressing pressure is 1.5MPa, the water content of the pressed mud cake is about 75%, and the filter pressing water is still turbid. The results show that after the thiobacillus ferrooxidans is subjected to bioleaching for 1d, chemical strengthening is performed, and because the pH is still high, oxidative degradation reaction is difficult to occur, and the sludge cannot be effectively and deeply dewatered.
Comparative example 4
In the same example 1, thiobacillus ferrooxidans liquid (10%, v/v) is inoculated into an anaerobic digestion sludge system, each treatment is provided with 3 groups of parallel experiments, the parallel experiments are respectively placed in constant-temperature shaking tables at 28 ℃ and 180r/min for culturing for 3d, the pH value of the sludge system is measured to be reduced to 3.0, the sludge is conveyed to a plate-and-frame filter press by a pump for direct filter pressing, the feeding time is 30min, the pressure is maintained for 30min by high-pressure water pressing, the feeding pressure is 0.7MPa, the diaphragm pressing pressure is 1.5MPa, the water content of a pressed mud cake is 80%, and the filter-pressed water is turbid. The results show that the thiobacillus ferrooxidans bioleaching treatment hardly effectively dehydrates sludge in 3 days of conventional reaction without adding ferrous ions.
Example 2
In the same example 1, Penicillium simplicissimum NAU-12 bacterial liquid (20%, v/v) is inoculated into an anaerobic digestion sludge system, 3 groups of parallel experiments are set for each treatment, the parallel experiments are respectively placed in a constant temperature shaking table at 28 ℃ and 180r/min for culturing for 1d, lost water is supplemented through a weighing and water supplementing method, the water content is kept about 95%, bioleaching modified sludge is obtained, the pH value of the sludge system is measured to be reduced to 2.8, and 60mg/g DS Fe is added 2+ And 30mg/g DS H 2 O 2 Respectively culturing in shaking table at constant temperature of 28 deg.C and 180r/min for 1 hr, and measuring pH of sludge to 2.7. Conveying the sludge subjected to the chemical enhanced bioleaching conditioning to a plate-and-frame filter press by using a pump for direct filter pressing, wherein the feeding time is 30min, the high-pressure water pressing and pressure maintaining are performed for 30min, the feeding pressure is 0.7MPa, the diaphragm pressing pressure is 1.5MPa, the filter pressing water is colorless and clear, the pressed mud cake is earthy yellow and odorless, and the water content of the mud cake is determined to be 55%.
Example 3
As in example 1, the bacterial solution (20%, v/v) of Penicillium simplicissimum NAU-12 was inoculated into the anaerobic digested sludge system at each siteArranging 3 groups of parallel experiments, respectively culturing in constant-temperature shaking tables at 28 ℃ and 180r/min for 1d, supplementing lost water by a weighing and water supplementing method, keeping the water content at about 95 percent to obtain bioleaching modified sludge, measuring the pH value of a sludge system to be reduced to 2.8, and then adding 60mg/g DS Fe 2+ And 30mg/g DS H 2 O 2 Respectively culturing in a shaking table with constant temperature of 28 ℃ and 180r/min for 2h, and measuring the pH value of the sludge to be 2.5. Conveying the sludge subjected to the chemical strengthening bioleaching conditioning to a plate-and-frame filter press by using a pump for direct filter pressing, wherein the feeding time is 30min, the high-pressure water pressing and pressure maintaining are carried out for 30min, the feeding pressure is 0.7MPa, the diaphragm pressing pressure is 1.5MPa, the filter pressing water is colorless and clear at the moment, the pressed mud cake is earthy yellow and odorless, and the water content of the mud cake is measured to be 54%.
The above description is illustrative of the present invention and its embodiments, and is not to be construed as limiting, and the embodiments shown in the examples are only one embodiment of the present invention, and the actual embodiments are not limited thereto. Therefore, if a person skilled in the art should appreciate that they can design embodiments and examples similar to the above-mentioned technical solutions without departing from the spirit of the present invention, and they should fall into the protection scope of the present invention.
Claims (10)
1. A method for chemically strengthening bioleaching treatment of anaerobic digestion sludge is characterized by comprising the following steps:
s1 bioleaching conditioned anaerobic sludge: inoculating the penicillium simplicissimum NAU-12 bacterial liquid into an anaerobic digested sludge system, and conditioning anaerobic sludge by adopting a bioleaching method; the Penicillium notatum NAU-12 is classified and named as Penicillium simplicissimum with the preservation number of CGMCC NO. 10990;
s2 chemical enhanced bioleaching conditioning: adding Fe 2+ And H 2 O 2 Culturing for a specific time;
s3, filter pressing dehydration: and (5) directly performing filter pressing on the sludge obtained in the step (S2) under a plate-and-frame filter press for solid-liquid separation to obtain sludge cakes with the water content of less than 60%.
2. Root of herbaceous plantThe method for bioleaching sludge with chemically enhanced anaerobic digestion according to claim 1, wherein the bacterial cell number of the bacterial solution of Penicillium simplicissimum NAU-12 used in the step S1 is 10 7 ～10 8 The inoculation volume is 1 to 20 percent of the anaerobic digestion sludge per mL.
3. The method for the bioleaching treatment of chemically enhanced anaerobic digestion sludge according to claim 1, wherein the pH of the system is reduced to below 3.0 after the cultivation in the step S2.
4. The method for bioleaching sludge with chemically enhanced anaerobic digestion according to claim 2, wherein the culture method of the penicillium simplicissimum NAU-12 bacterial liquid in the step S1 is as follows: inoculating the penicillium simplicissimum NAU-12 separated from the sludge into a martin solid culture medium, and then placing the solid culture medium into a reciprocating shaking table at 28 ℃ and 180-200 rpm for vibration propagation culture until the number of the bacterial cells reaches 10 7 ～10 8 One cell per mL, stored at 4 ℃ for use.
5. The method for bioleaching sludge with chemically enhanced anaerobic digestion according to claim 4, wherein the Martin's solid medium is prepared by the following method: sequentially dissolving 1.0g of monopotassium phosphate, 0.5g of magnesium sulfate heptahydrate, 5.0g of peptone and 10.0g of glucose, adding distilled water to supplement the volume to 1000mL, then adding 3.3mL of 1% Bengal red solution, adding 15-20 g of agar, and sterilizing for 30min by high-pressure steam at 115 ℃; before use, 0.3mL of streptomycin solution at 1% was added to 100mL of the medium.
6. The method for bioleaching anaerobic sludge with chemically enhanced digestion according to claim 2, wherein the bioleaching anaerobic sludge in the step S1 is carried out for 1-2 days.
7. The method for chemically enhanced bioleaching anaerobic digestion sludge as claimed in claim 2, wherein Fe in step S2 2+ And H 2 O 2 The mass ratio is 2: 1.
8. the method for bioleaching sludge with chemically enhanced anaerobic digestion according to claim 7, wherein the time for the chemically enhanced bioleaching conditioning in the step S2 is 1-2 hours.
9. The method for bioleaching sludge with chemically enhanced anaerobic digestion according to claim 7, wherein the step S1 is specifically as follows: inoculating the penicillium simplicissimum NAU-12 bacterial liquid into an anaerobic digestion sludge system, culturing in a constant-temperature shaking table at 28 ℃ and 180r/min for 1d, supplementing water lost every day by a weighing and water supplementing method, taking a sludge sample after culturing, and measuring the pH value of the sludge.
10. The method for bioleaching sludge with chemically enhanced anaerobic digestion according to any one of claims 1 to 9, wherein the step S2 is specifically: after the sludge is treated by bioleaching for 1d, Fe is added 2+ And H 2 O 2 In which Fe 2+ And H 2 O 2 The mass ratio is 2: 1, placing the mixture into a constant-temperature shaking table with the temperature of 28 ℃ and the speed of 180r/min for culturing for 1-2 h, and taking a sludge sample to measure the pH of the sludge.
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