CN108083597B - Composite microbial liquid for treating anaerobic digestion sludge and novel bioleaching method - Google Patents

Composite microbial liquid for treating anaerobic digestion sludge and novel bioleaching method Download PDF

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CN108083597B
CN108083597B CN201711449769.1A CN201711449769A CN108083597B CN 108083597 B CN108083597 B CN 108083597B CN 201711449769 A CN201711449769 A CN 201711449769A CN 108083597 B CN108083597 B CN 108083597B
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周立祥
李浙英
颜成
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Nanjing Beikete Environmental Protection Technology Co ltd
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Abstract

The invention discloses a bioleaching method for anaerobic digestion sludge, belonging to the technical field of sludge treatment. According to the method, a new microbial strain is introduced to compound a bioleaching working flora, continuous domestication is carried out, an inoculum obtained by domestication is mixed with anaerobic digested sludge to carry out bioleaching treatment, the new microbial strain is used for degrading micromolecular organic acid and secreting surface active substances to rapidly strip EPS wrapped on the surfaces of sludge cells, the bioleaching reaction time is shortened to 12-24 hours, the sludge after bioleaching is directly dewatered by a plate-and-frame filter press, and mud cakes with the water content lower than 60% are obtained. The method has simple process, fully utilizes the action of microorganisms without adding any chemical agent, and has the advantages of high dehydration efficiency, low operation cost and small process operation difficulty.

Description

Composite microbial liquid for treating anaerobic digestion sludge and novel bioleaching method
Technical Field
The invention belongs to the technical field of sludge treatment, and particularly relates to a novel method for treating anaerobic digestion sludge composite microbial liquid and bioleaching.
Background
In recent years, with the acceleration of urbanization progress and the improvement of environmental quality standards in China, the amount of sewage to be treated is increased rapidly, so that various sewage treatment processes such as oxidation ditches, biological filters and oxidation ponds are widely applied at the present stage, and finally, a large amount of another pollutant (sludge) is generated in sewage treatment. The components of the sludge are very complex, the difference of the components of the sludge from different sources is huge, the sludge mainly comprises zoogloea formed by various microorganisms and a set formed by absorbed organic and inorganic substances, the operation and treatment effect of a sewage treatment plant can be ensured only by timely and effectively treating and disposing the sludge, and the sludge treatment becomes a potential risk and an environmental problem which are increasingly prominent and need to be solved urgently. Sludge dewatering can effectively reduce the volume of sludge to be treated, is the most economical method in sludge reduction, and is also an essential link for sludge final treatment such as incineration, composting and the like.
At present, the commonly used sludge deep dehydration method mainly adopts the method of adding organic or inorganic flocculating agents into a sludge system, wherein the inorganic flocculating agents mainly comprise ferric salt, aluminum salt and the like, and the organic flocculating agents mainly comprise cationic polymeric flocculating agents (PAM-DMC) and Polyacrylamide (PAM). The polyacrylamide is a flocculant widely used in sewage treatment plants at present, is mostly used together with lime and ferric trichloride, and is dehydrated to the water content of below 60 percent by adopting a chamber filter press. The method has many defects in practical application, namely, the operation cost is high, the using amount of the inorganic medicament is large, and 60 percent of the dry matter of the sludge needs to be reached; secondly, the heat value of the treated sludge is greatly reduced, and the subsequent resource utilization of the sludge is not facilitated; and thirdly, after limestone is added, the COD and ammonia nitrogen content are increased, the sewage treatment load is increased, and the pH value of the sludge is increased to 12-13.
The bioleaching technology is a new sludge deep dehydration technology which is newly emerged in recent years, sludge treatment process completely utilizes special bioleaching microorganisms to carry out dehydration performance modification on sludge under aeration conditions, and the sludge is directly subjected to pressure filtration by a plate-and-frame filter press until the water content of a sludge cake is lower than 60 percent, so that the bioleaching technology has the advantages of low operation cost, sludge organic matter and heat value preservation, no increase of the dry matter amount of the dehydrated sludge, complete adoption of the microorganisms, no addition of any flocculating agent or coagulant aid, and complete removal of sludge stink and pathogenic bacteria, and is an environment-friendly sludge treatment technology.
In view of the obvious advantages of the bioleaching technology in deep dehydration of sludge, the technology is widely applied to sludge treatment of urban sewage treatment plants, sludge treatment projects are formed on the aspects of stannlessness, Harbin, Tongxiang, Ningbo and the like, and the daily sludge treatment reaches 3000 million tons. Through retrieval, Chinese patent application No. 201410119833.X, patent application publication No. 2014 7/23 discloses a novel method for quickly conditioning deep dehydration by a sludge bioleaching method, which comprises the steps of firstly performing bioleaching conditioning on sludge, namely directly pumping concentrated liquid sludge of a sewage treatment plant into a bioleaching reactor containing a special composite microbial flora for treatment for 1.5-2d, changing bound water in the sludge into free water which is easy to remove through microbial substitution effect, biological oxidation and biological acidification, and simultaneously dissolving heavy metals in the sludge into water phase and killing pathogens in the sludge. Then collecting the sludge after bioleaching in a homogenizing pool, and directly conveying the sludge to a diaphragm box type filter press with common pressure through a pump for dehydration, wherein the water content of a dehydrated sludge cake is lower than 60%. The composite microbial flora used in the patent is thiobacillus thiooxidans TS6, thiobacillus ferrooxidans LX5, pichia pastoris Z3 and rhodotorula benthica R30, the applicable environment is undigested common municipal sludge, such as concentrated sludge, the pH of the sludge subjected to bioleaching treatment can be quickly reduced, the negative charges on the surfaces of sludge particles can be reduced, the effect of weakening the repulsion force among the sludge particles is achieved, the sludge dehydration property is enhanced finally, and the deep dehydration effect is achieved. Anaerobic digestion is a commonly used process for sludge stabilization in sewage treatment plants for the purpose of killing pathogenic bacteria, reducing volatile solids and removing malodors. Anaerobic digestion is divided into three stages, namely a hydrolysis fermentation stage, a hydrogen-producing and acetic-acid-producing stage and a methane-producing stage, the methane yield can be increased by hydrolyzing sludge before digestion, organic molecules and molecular compounds which are difficult to decompose are degraded into small molecular organic acids such as formic acid, acetic acid, propionic acid, butyric acid and the like, the sludge after anaerobic digestion contains suspended matters, ammonia nitrogen, phosphorus and a large amount of Extracellular Polymeric Substances (EPS), the pH value of the sludge is alkaline and the like. In practice, we find that the conventional bioleaching method for directly treating anaerobic digestion sludge cannot achieve the expected effect, the dehydration performance cannot be improved, the specific resistance increases along with the treatment time, and the pH value of a sludge system does not decrease but increases instead.
Because the traditional bioleaching process cannot improve the dehydration performance of anaerobic digestion sludge and cannot achieve the effect of deep dehydration, a new bioleaching method suitable for anaerobic digestion sludge is urgently needed to be found so as to improve the bioleaching dehydration efficiency and realize the aim of realizing reduction of the deep dehydration of the anaerobic digestion sludge.
Disclosure of Invention
1. Problems to be solved
Aiming at the phenomenon that the sludge specific resistance and the pH value are not reduced but are increased to cause the deterioration of the sludge dewatering effect when the anaerobic digested sludge is directly treated by the traditional bioleaching method, the invention provides a novel bioleaching method of the anaerobic digested sludge, which adopts a compound bacterial liquid consisting of thiobacillus ferrooxidans LX5, thiobacillus thiooxidans TS6, Bordetella ZW2 and Pichia pastoris D13 to carry out bioleaching on the anaerobic digested sludge.
2. Technical scheme
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a novel bioleaching method for anaerobic digestion sludge comprises the following steps:
(1) composite microbial liquid culture
Respectively inoculating thiobacillus ferrooxidans LX5(A. ferrooxidans LX5 with the preservation number of CGMCCNo.0727), thiobacillus thiooxidans TS6(A. thiooxidans TS6 with the preservation number of CGMCC NO.0759), Bordetella ZW2(Bordetella sp. preservation number of CGMCC NO.3033) and Pichia pastoris D13(P. sparatin D13 with the preservation number of CGMCCNO.3035) separated from sludge into respective culture media, and then placing the culture media into a reciprocating shaking table at 23-28 ℃ and 180-200 rpm for carrying out oscillatory propagation culture until the cell number of thalli reaches 108Per mL; then, mixing thiobacillus ferrooxidans LX5, thiobacillus thiooxidans TS6, bordetella ZW2 and pichia pastoris D13 culture solutions in a volume ratio of (1-3): (2-5): (1-3): (1-3) mixing to obtain a compound microbial liquid;
(2) domestication of compound microorganism bacterium liquid
Inoculating the composite microbial inoculum into an anaerobic digestion sludge system, adding a microbial nutrient inoculum, and culturing and domesticating at 23-28 ℃ under an aeration condition until the pH value of the sludge system is reduced to 3.0, and stopping culturing to obtain a first batch of acidified sludge; then inoculating the first batch of acidified sludge to fresh anaerobic digested sludge, adding a microbial nutrient agent, and culturing and domesticating at 23-28 ℃ under an aeration condition until the pH value of a sludge system is reduced to 3.0; repeating the above process to domesticate the compound microorganism bacteria liquid;
(3) bioleaching conditioning
Mixing the domesticated compound microorganism bacterium liquid and anaerobic digestion sludge according to the volume ratio of 1: (5-10) mixing the raw materials into a bioleaching reactor, adding a microbial nutrition agent, and bioleaching for 12-24 hours at the temperature of 23-28 ℃ under aeration and stirring conditions to obtain bioleaching sludge;
(4) filter pressing dehydration
And (3) directly performing filter pressing on the sludge subjected to bioleaching conditioning under a plate-and-frame filter press for solid-liquid separation to obtain a sludge cake with the water content of less than 60%.
Further, the culture medium g/L of Thiobacillus ferrooxidans LX 5: (NH)4)2SO43.5，KCl 0.119，K2HPO40.58，Ca(NO3)2·4H2O 0.168，MgSO4·7H2O4.42, use 10N H2SO4Adjusting the pH value to 2.5; thiobacillus thiooxidans TS6 medium g/L: (NH4)2SO40.4，KH2PO43.0，MgSO4·7H2O 0.5，CaCl2·2H20.25 of O, 10 of sulfur powder; bordetella ZW2 culture medium g/L: (NH)4)2SO40.3，CaCl2·2H2O 0.3，KCl 0.5，ZnSO40.3，MnSO40.23; pichia pastoris D13 medium g/L: potato powder 200, glucose 20, 10N H2SO4The pH was adjusted to 2.5.
Furthermore, the microbial nutrient inoculum is a solid substance obtained by taking high-nutrient substances as raw materials, performing microbial fermentation, filter pressing, dehydration, drying and crushing, wherein main nutrient elements comprise active thalli and nutrient elements containing N, P, K, Fe, Zn, Mn, S and the like.
Furthermore, the adding volume of the microbial nutrient inoculant is 2-5% of that of anaerobic digestion sludge.
Further, the composite microbial liquid in the step (2) is inoculated according to 1-10% of the anaerobic digestion sludge volume; repeating the above process for 3-5 times in step (2) to acclimate the complex microorganism liquid.
Further, the aeration condition means that the aeration amount is controlled to 3～6m3/(h·m2)。
Furthermore, the water content of the anaerobic digestion sludge is controlled to be more than 98%.
A composite microbial liquid for treating anaerobic digested sludge is prepared from Thiobacillus ferrooxidans LX5, Thiobacillus thiooxidans TS6, Bordetella bacteria ZW2, and Pichia pastoris D13.
Furthermore, the ratio of the number of the bacteria of the thiobacillus ferrooxidans LX5, the thiobacillus thiooxidans TS6, the bordetella ZW2 and the pichia pastoris D13 is (1-3): (2-5): (1-3): (1-3).
Use of Bordetella ZW2(Bordetella sp.) in the field of anaerobic digestion of sludge treatment.
The bioleaching reactor is a plug flow bioleaching reactor, preferably a plug flow bioleaching treatment device disclosed in the patent application with the application number of 200810242584.8.
3. Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention introduces a new microbial strain to compound a bioleaching working flora, carries out continuous domestication, utilizes the new microbial strain Bordetella sp ZW2 to degrade micromolecular organic acid and secrete surface active substances to rapidly peel off EPS wrapped on the surface of sludge cells, eliminates the toxic inhibition effect on bioleaching acidophilic thiobacillus, rapidly propagates in an anaerobic digestion sludge system, plays a role in biological acidification and ensures the bioleaching dehydration efficiency.
(2) The bioleaching conditioning for treating anaerobic digestion sludge only needs 12-24 hours, compared with the traditional bioleaching, the bioleaching conditioning time is shortened by 12-24 hours, and the investment and operation cost is directly reduced.
(3) The microbial nutrient agent is used as an energy substance of the complex flora, the utilization rate is high, the usage amount is small, compared with the traditional microbial nutrient agent, the microbial nutrient agent is reduced by 50-80%, and the bioleaching operation cost is effectively reduced.
(4) The domesticated compound microorganism flora is directly added into the sludge without separate culture, so that the process is simplified and the actual operation difficulty is reduced.
(5) The domesticated compound microorganism only needs to be added once, and the operation is long-term, so that the operation cost is saved.
(6) The method has simple process, completely adopts a microbial method, does not need to add any chemical agent, does not influence key indexes such as heat value, organic matters and the like of the sludge, can realize subsequent resource utilization diversity and generates certain economic value.
(7) Acidophilic thiobacillus cannot survive in an alkaline environment, has no biological acidification effect, and the bioleaching cannot improve the sludge dewatering effect; researches show that the Bordetella ZW2(Bordetella sp. preservation number CGMCC NO.3033) separated from the sludge can degrade small molecular organic substances and secrete surface active substances to rapidly strip EPS, including proteins, polysaccharides and nucleic acids possibly generated by cell lysis, and has an important effect on improving the sludge dewatering effect.
Drawings
FIG. 1 is a process flow diagram of a novel bioleaching process for anaerobically digested sludge in accordance with the present invention.
Proof of biological Material preservation
Thiobacillus ferrooxidans LX5, classified as thiobacillus ferrooxidans, is preserved in China general microbiological culture Collection center (CGMCC), the preservation address is China Zhongguancun, the preservation date is 3 months and 13 days in 2002, and the preservation number is CGMCC No. 0727.
Thiobacillus thiooxidans TS6, classified as Thiobacillus thiooxidans, is preserved in China general microbiological culture Collection center (CGMCC), the preservation address is China Zhongguancun, the preservation date is 7/4/2002, and the preservation number is CGMCC No. 0759.
Pichia pastoris D13, classified as Pichia spartinae, is deposited in China general microbiological culture Collection center, with the deposit address of Datun road in the Cynanchum Kogyuchi of Beijing, China academy of sciences, the deposit date of 2009, 4 months and 23 days, and the deposit number of CGMCC No. 3035.
Bordetella ZW2 with a classification name of Bordetella sp, is preserved in the China general microbiological culture Collection center, the preservation address is Shangyang district Tutun road in Beijing, China academy of sciences, the preservation date is 2009, 4 and 23 days, and the preservation number is CGMCC NO. 3033.
Detailed Description
The invention is further described with reference to specific examples.
Example 1
Test for bioleaching treatment of anaerobic digestion sludge of Nanjing sewage treatment plant
According to the process, the anaerobic digestion sludge of the sewage treatment plant is treated according to the flow (see attached figure 1), about 20L of the anaerobic digestion sludge of the plant is taken, and the basic properties of the sludge are determined as follows, namely the pH value is 7.10, the solid content is 4.06%, the organic matter is 48.9%, and the specific resistance of the sludge is 6.8 × 1013m/kg, total nitrogen 35.1%, total phosphorus 27%.
(1) Method for culturing compound microbial flora by utilizing anaerobic digestion sludge
Respectively inoculating Thiobacillus ferrooxidans LX5(A.ferrooxidans LX5 with the preservation number of CGMCC NO.0727), Thiobacillus thiooxidans TS6(A.thiooxidans TS6 with the preservation number of CGMCC NO.0759), Bordetella ZW2(Bordetella sp. with the preservation number of CGMCC NO.3033) and Pichia pastoris D13(P.spartinae D13 with the preservation number of CGMCC NO.3035) into respective culture medium (Thiobacillus ferrooxidans LX5 culture medium g/L (NH)4)2SO43.5，KCl 0.119，K2HPO40.58，Ca(NO3)2·4H2O 0.168，MgSO4·7H2O4.42, use 10N H2SO4Adjusting the pH value to 2.5; thiobacillus thiooxidans TS6 medium g/L: (NH4)2SO40.4，KH2PO43.0，MgSO4·7H2O 0.5，CaCl2·2H20.25 of O, 10 of sulfur powder; bordetella ZW2 culture medium g/L: (NH)4)2SO40.3，CaCl2·2H2O 0.3，KCl 0.5，ZnSO40.3，MnSO40.23; pichia pastoris D13 medium g/L: potato powder 200, glucose 20, 10N H2SO4Adjusting pH to 2.5), placing in a reciprocating shaker at 25 deg.C and 180rpmCarrying out oscillatory propagation culture until the cell number of the thallus reaches 108one/mL. Carrying out mixing on thiobacillus ferrooxidans LX5, thiobacillus thiooxidans TS6, bordetella ZW2 and pichia pastoris D13 culture solution according to the volume ratio of 2: 4: 3: 3, inoculating the composite microbial inoculum into an anaerobic digestion sludge system (the volume of the inoculum is 10mL and the volume of the anaerobic digestion sludge is 100mL), adding 2g of microbial nutrient inoculum, and aerating at 25 ℃ (aeration rate is 3 m)3/(h·m2) Culturing and domesticating are carried out until the pH value of the sludge system is reduced to 3.0, and the culture is stopped to obtain a first batch of acidified sludge. Then taking 100mL of the first acidified sludge to inoculate into 1000mL of fresh anaerobic digested sludge, adding 2g of microbial nutrient inoculum, and aerating at 25 ℃ (aeration amount is 3 m)3/(h·m2) Culturing and domesticating until the pH value of the sludge system is reduced to 3.0. Repeating the above process for 5 times to obtain acclimatized sludge as inoculum.
The preparation method of the microbial nutrient inoculum comprises the following steps:
(a) obtaining the compound microbial flora
Respectively inoculating Thiobacillus ferrooxidans LX5(A. ferrooxidans LX5 with the preservation number of CGMCC NO.0727) and Bordetella ZW2(Bordetella sp., the preservation number of CGMCC NO.3033) into respective culture medium, and then placing the culture medium at 25 ℃ under the aeration condition (3 m)3/(h·m2) Propagation culture until the cell number of the thallus reaches 107one/mL. The method comprises the following steps of (1) mixing thiobacillus ferrooxidans LX5 and bordetella ZW2 culture solution in a volume ratio of 3: 1, mixing to obtain the compound microbial liquid. The total volume of the compound microbial inoculum is 5L.
(b) Fermentation of nutrient complex flora
Weighing 50g of sucrose, 10g of peptone, 100g of potassium dihydrogen phosphate, 40g of ammonium chloride, 10g of ammonium hydrogen phosphate, 50g of ammonium sulfate, 30g of magnesium sulfate heptahydrate, 50g of sodium thiosulfate pentahydrate, 10g of calcium nitrate, 50g of ferric sulfate, 10g of manganese sulfate, 100g of zinc sulfate, 40g of potassium chloride, 200g of ferrous sulfate heptahydrate, 10g of ammonium sulfate and 50g of sulfur.
Dissolving the above nutrients in 10L water, adding compound microorganism solution, and aerating at 25 deg.C (3 m)3/(h·m2) Culturing is carried out until the pH of the culture system is reduced to 3.0.
(c) Solid-liquid separation
Separating the mixture fermented by the composite flora by solid-liquid separation equipment, and crushing the fermented nutritional mud cake to below 100 meshes by adopting crushing equipment.
(d) Mixing of auxiliary materials
And (3) putting the fermented nutrient and filler diatomite into a stirrer, fully mixing and uniformly stirring to obtain the nutrient microbial inoculum.
(2) Bioleaching process
Diluting anaerobic digestion sludge to be treated to reach the water content of 98%, and mixing the inoculum and the anaerobic digestion sludge according to the volume ratio of 1: 10 placing the inoculum and anaerobic digestion sludge into a bioleaching reactor, and aerating at 25 deg.C (aeration rate of 3 m)3/(h·m2) Bioleaching for 12 hours to obtain bioleaching modified sludge, and measuring that the pH value of a sludge system is reduced to 2.0.
(3) Filter pressing dehydration
Conveying the sludge conditioned by bioleaching to a plate-and-frame filter press by a pump for direct filter pressing, wherein the feeding time is 30min, the high-pressure water pressing and pressure maintaining are carried out for 45min, the feeding pressure is 0.6MPa, the diaphragm pressing pressure is 1.0MPa, the filter pressing water is colorless and clear, the pressed mud cake is earthy yellow and odorless, and the water content of the mud cake is determined to be 58.4%.
Comparative example 1
The anaerobic digestion sludge is treated by adopting the traditional bioleaching microbial flora (no bordetella ZW2 is added), the treatment steps are the same as the example 1, the bioleaching is carried out for 24 hours, the pH value of a sludge system is 8.6, the sludge is conveyed to a plate-and-frame filter press by a pump to be directly pressed and filtered, the sludge feeding amount is 20L after 1 hour, the pressed sludge cake is unshaped, black and odorous, the water content of the sludge cake is determined to be 85%, and the pressed and filtered water is muddy water.
Example 2
Anaerobic digestion sludge bioleaching treatment test of stannless sewage treatment plant
According to the process, anaerobic digestion sludge of the sewage treatment plant is treated according to a flow path (see attached figure 1). Taking about 100L ofThe basic properties of the anaerobic digested sludge of a factory are determined as follows, the pH value is 7.56, the solid content is 3.0%, the organic matter is 50.1%, and the sludge specific resistance is 8.37 × 1013m/kg, total nitrogen 35.1%, total phosphorus 27%.
(1) Method for culturing compound microbial flora by utilizing anaerobic digestion sludge
Respectively inoculating Thiobacillus ferrooxidans LX5, Thiobacillus thiooxidans TS6, Bordetella ZW2 and Pichia pastoris D13 into respective culture medium (Thiobacillus ferrooxidans LX5 culture medium g/L (NH)4)2SO43.5，KCl 0.119，K2HPO40.58，Ca(NO3)2·4H2O 0.168，MgSO4·7H2O4.42, use 10N H2SO4Adjusting the pH value to 2.5; thiobacillus thiooxidans TS6 medium g/L: (NH4)2SO40.4，KH2PO43.0，MgSO4·7H2O 0.5，CaCl2·2H20.25 of O, 10 of sulfur powder; bordetella ZW2 culture medium g/L: (NH)4)2SO40.3，CaCl2·2H2O 0.3，KCl 0.5，ZnSO40.3，MnSO40.23; pichia pastoris D13 medium g/L: potato powder 200, glucose 20, 10N H2SO4Adjusting pH to 2.5), placing in a reciprocating shaking table at 23 deg.C and 200rpm, and performing vibration propagation culture until the cell number reaches 108one/mL. Carrying out mixing on thiobacillus ferrooxidans LX5, thiobacillus thiooxidans TS6, bordetella ZW2 and pichia pastoris D13 culture solution according to the volume ratio of 1: 5: 2: 2 obtaining a compound microorganism bacterium liquid by mixing, inoculating the compound microorganism bacterium liquid into an anaerobic digestion sludge system (the volume of the bacterium liquid is 50mL, and the volume of the anaerobic digestion sludge is 500mL), adding 15g of microorganism nutrient bacteria agent, and aerating at 23 ℃ (aeration amount is 6 m)3/(h·m2) Culturing and domesticating are carried out until the pH value of the sludge system is reduced to 3.0, and the culture is stopped to obtain a first batch of acidified sludge. Then, 500mL of the first acidified sludge is taken and inoculated to 5000mL of fresh anaerobic digested sludge, 10g of microbial nutrient inoculum is added, and the mixture is aerated at the temperature of 25 ℃ (the aeration amount is 6 m)3/(h·m2) Culturing and domesticating until the pH value of the sludge system is reduced to 3.0. Repeating the above processAnd 3 times, wherein the obtained acidified sludge is an acclimatized inoculum.
(2) Bioleaching process
Diluting anaerobic digestion sludge to be treated to water content of 98%, placing inoculum and anaerobic digestion sludge into a bioleaching reactor (formed by welding 4mm steel plates, long rectangular structure with the size of 1000mm × 800mm × 600mm and the working volume of 400L, completely dividing the anaerobic digestion sludge into 2 galleries by the steel plates along the longitudinal direction, arranging 2 baffle plates in each gallery, staggering up and down, dividing the galleries into 3 areas, and arranging a small-hole tubular aerator in the galleries according to the volume ratio of the inoculum to the anaerobic digestion sludge of 1: 5, wherein the small-hole tubular aerator is arranged in the small-hole tubular aerator, and the concrete structure of the small-hole tubular aerator is disclosed in the application No. 200810242584.8 patent), and under the aeration condition at 23 ℃ (the aeration amount of 6m3/(h·m2) Bioleaching for 24 hours to obtain bioleaching modified sludge, and measuring that the pH value of a sludge system is reduced to 2.0.
(3) Filter pressing dehydration
Conveying the sludge conditioned by bioleaching to a plate-and-frame filter press by a pump for direct filter pressing, wherein the feeding time is 60min, the high-pressure water pressing and pressure maintaining are carried out for 90min, the feeding pressure is 0.6MPa, the diaphragm pressing pressure is 1.0MPa, the filter pressing water is colorless and clear, the pressed mud cake is earthy yellow and odorless, and the water content of the mud cake is determined to be 58.3%.
Example 3
Zhengzhou sewage treatment plant anaerobic digestion sludge bioleaching treatment test
According to the process, anaerobic digested sludge of the sewage treatment plant is treated according to the flow (see attached figure 1), about 500L of anaerobic digested sludge of the plant is measured, and the basic properties of the sludge are that the pH value is 7.32, the solid content is 3.6%, the organic matter is 48.6%, and the specific sludge resistance is 6.37 × 1013m/kg, total nitrogen 33.1%, total phosphorus 25%.
(1) Method for culturing compound microbial flora by utilizing anaerobic digestion sludge
Respectively inoculating Thiobacillus ferrooxidans LX5, Thiobacillus thiooxidans TS6, Bordetella ZW2 and Pichia pastoris D13 into respective culture medium (Thiobacillus ferrooxidans LX5 culture medium g/L (NH)4)2SO43.5，KCl 0.119，K2HPO40.58，Ca(NO3)2·4H2O 0.168，MgSO4·7H2O4.42, pH adjusted to 2.5 with 10N H2SO 4; thiobacillus thiooxidans TS6 medium g/L: (NH4)2SO40.4，KH2PO43.0，MgSO4·7H2O 0.5，CaCl2·2H20.25 of O, 10 of sulfur powder; bordetella ZW2 culture medium g/L: (NH)4)2SO40.3，CaCl2·2H2O 0.3，KCl 0.5，ZnSO40.3，MnSO40.23; pichia pastoris D13 medium g/L: potato powder 200, glucose 20, 10N H2SO4Adjusting pH to 2.5), placing in a reciprocating shaker at 28 deg.C and 200rpm, and performing vibration propagation culture until the cell number reaches 108one/mL. Mixing thiobacillus ferrooxidans LX5, thiobacillus thiooxidans TS6, bordetella ZW2 and pichia pastoris D13 culture solution according to the volume ratio of 3: 2: 1: 1, inoculating the composite microbial inoculum into an anaerobic digestion sludge system (the volume of the inoculum is 50mL and the volume of the anaerobic digestion sludge is 500mL), adding 25g of microbial nutrient inoculum, and aerating at 28 ℃ under aeration conditions (the aeration rate is 5 m)3/(h·m2) Culturing and domesticating are carried out until the pH value of the sludge system is reduced to 3.0, and the culture is stopped to obtain a first batch of acidified sludge. Then, 500mL of the first acidified sludge is taken and inoculated to 5000mL of fresh anaerobic digested sludge, 15g of microbial nutrient inoculum is added, and the mixture is aerated at the temperature of 28 ℃ (aeration rate is 5 m)3/(h·m2) Culturing and domesticating until the pH value of the sludge system is reduced to 3.0. Repeating the above process for 5 times to obtain acclimatized sludge as inoculum.
(2) Bioleaching process
Diluting anaerobic digestion sludge to be treated to reach the water content of 98%, and mixing the inoculum and the anaerobic digestion sludge according to the volume ratio of 1: 8 the inoculum and anaerobically digested sludge are placed into a bioleaching reactor. Under the condition of aeration at 28 ℃ (aeration rate is 5 m)3/(h·m2) Bioleaching for 18h to obtain bioleaching modified sludge, and measuring that the pH value of a sludge system is reduced to 2.5.
(3) Filter pressing dehydration
Conveying the sludge conditioned by bioleaching to a plate-and-frame filter press by a pump for direct filter pressing, wherein the feeding time is 90min, the high-pressure water pressing and pressure maintaining are carried out for 120min, the feeding pressure is 0.6MPa, the diaphragm pressing pressure is 1.0MPa, the filter pressing water is colorless and clear, the pressed mud cake is earthy yellow and odorless, and the water content of the mud cake is 57.9% by determination.

Claims (7)

1. A novel bioleaching method for anaerobic digestion sludge is characterized by comprising the following steps:
(1) composite microbial liquid culture
Respectively inoculating thiobacillus ferrooxidans LX5, thiobacillus thiooxidans TS6, bordetella ZW2 and pichia pastoris D13 separated from sludge into respective culture media, and then placing the culture media in a reciprocating type shaking table at 23-28 ℃ and 180-200 rpm for vibration propagation culture until the number of somatic cells reaches 108Per mL; then, mixing thiobacillus ferrooxidans LX5, thiobacillus thiooxidans TS6, bordetella ZW2 and pichia pastoris D13 culture solutions in a volume ratio of (1-3): (2-5): (1-3): (1-3) mixing to obtain a compound microbial liquid;
(2) domestication of compound microorganism bacterium liquid
Inoculating the composite microbial inoculum into an anaerobic digestion sludge system, adding a microbial nutrient inoculum, and culturing and domesticating at 23-28 ℃ under an aeration condition until the pH value of the sludge system is reduced to 3.0, and stopping culturing to obtain a first batch of acidified sludge; then inoculating the first batch of acidified sludge to fresh anaerobic digested sludge, adding a microbial nutrient agent, and culturing and domesticating at 23-28 ℃ under an aeration condition until the pH value of a sludge system is reduced to 3.0; repeating the above process to domesticate the compound microorganism bacteria liquid;
(3) bioleaching conditioning
Mixing the domesticated compound microorganism bacterium liquid and anaerobic digestion sludge according to the volume ratio of 1: (5-10) mixing the raw materials into a bioleaching reactor, adding a microbial nutrition agent, and bioleaching for 12-24 hours at the temperature of 23-28 ℃ under aeration and stirring conditions to obtain bioleaching sludge; the microbial nutrient inoculum is a solid substance obtained by taking high-nutrient substances as raw materials and performing filter pressing and dehydration after microbial fermentation, and the main nutrient elements comprise N, P, K and trace elements of Fe, Zn and Mn; the adding volume of the microbial nutrient inoculant is 2-5% of that of anaerobic digestion sludge;
(4) filter pressing dehydration
And (3) directly performing filter pressing on the sludge subjected to bioleaching conditioning under a plate-and-frame filter press for solid-liquid separation to obtain a sludge cake with the water content of less than 60%.
2. The novel bioleaching process for anaerobically digested sludge as claimed in claim 1, wherein the culture medium g/L of Thiobacillus ferrooxidans LX5 is: (NH)4)2SO43.5，KCl 0.119，K2HPO40.58，Ca(NO3)2·4H2O 0.168，MgSO4·7H2O4.42, use 10N H2SO4Adjusting the pH value to 2.5; thiobacillus thiooxidans TS6 medium g/L: (NH4)2SO40.4，KH2PO43.0，MgSO4·7H2O 0.5，CaCl2·2H20.25 of O, 10 of sulfur powder; bordetella ZW2 culture medium g/L: (NH)4)2SO40.3，CaCl2·2H2O 0.3，KCl 0.5，ZnSO40.3，MnSO40.23; pichia pastoris D13 medium g/L: potato powder 200, glucose 20, 10N H2SO4The pH was adjusted to 2.5.
3. The novel bioleaching method for anaerobic digestion sludge according to claim 1, characterized in that the composite microbial liquid in the step (2) is inoculated according to 1-10% of the volume of the anaerobic digestion sludge; repeating the above process for 3-5 times in step (2) to acclimate the complex microorganism liquid.
4. The novel bioleaching process for anaerobically digested sludge as claimed in claim 1, wherein: the aeration condition means that the aeration amount is controlled to be 3-6 m3/(h·m2)。
5. The novel bioleaching process for anaerobically digested sludge as claimed in claim 1, wherein: the water content of the anaerobic digestion sludge is controlled to be more than 98%.
6. The composite microbial liquid for treating anaerobic digested sludge is characterized by being prepared by compounding thiobacillus ferrooxidans LX5, thiobacillus thiooxidans TS6, bordetella ZW2 and pichia pastoris D13.
7. The composite microbial inoculum for treating anaerobic digested sludge according to claim 6, wherein the ratio of the number of bacteria of Thiobacillus ferrooxidans LX5, Thiobacillus thiooxidans TS6, Bordetella ZW2 and Pichia pastoris D13 is (1-3): (2-5): (1-3): (1-3).
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