CN114916536B - Device for preserving umbilical cord mesenchymal stem cells - Google Patents

Abstract

The invention relates to the technical field of stem cell storage, in particular to a device for storing umbilical cord mesenchymal stem cells, which comprises a storage shell, a cryopreservation placing unit, an access transmission mechanism, a cryopreservation buffer rack, a shielding compaction sealing mechanism and a magnetic automatic access mechanism, wherein the storage shell is a hollow cavity with an upper opening, the cryopreservation placing unit is arranged in the storage shell, the access transmission mechanism is arranged outside the cryopreservation placing unit, the cryopreservation buffer rack is radially arranged on the cryopreservation placing unit, the shielding compaction sealing mechanism is arranged in the storage shell, and the shielding compaction sealing mechanism is arranged at the upper part of the cryopreservation placing unit. According to the invention, the access of the cryopreserved stem cell cryopreserving tube is automatically controlled, the convenience of the device operation is increased, the exposed caliber of liquid nitrogen contacting with the outside is reduced, and thus the loss of the liquid nitrogen is reduced.

Description

Device for preserving umbilical cord mesenchymal stem cells

Technical Field

The invention belongs to the technical field of stem cell storage, and particularly relates to a device for storing umbilical cord mesenchymal stem cells.

Background

Umbilical cord Mesenchymal Stem Cells (MSCs) refer to a multifunctional Stem cell existing in umbilical cord tissues of newborns, can be differentiated into a plurality of tissue Cells, and has wide clinical application prospect. The umbilical cord mesenchymal stem cells are clinically applied to the aspects of solving various blood system diseases, cardiovascular diseases, liver cirrhosis, nervous system diseases, repair of partial resection injury of knee joints, autoimmune diseases and the like, and make great breakthrough.

After the human umbilical cord mesenchymal stem cells are separated and cultured from the umbilical cord tissue of the newborn, the human umbilical cord mesenchymal stem cells are detected and identified, and then the human umbilical cord mesenchymal stem cells are frozen and stored in a set place so as to be directly and conveniently taken out for later use.

Present cryopreserved device adopts the liquid nitrogen to cryopreserve the pipe with stem cell, need open whole device when taking, the device is inside to be exposed outside, it is great to receive outside influence, the staff need wear frostproofing gloves and stretch into the device inside and take when taking, during the opening device, "smog" can influence the staff sight, if the stem cell cryopreserved pipe quantity of depositing in the device is more, it is relatively difficult to seek, it is extremely inconvenient to use, and stretch into the refrigerated environment with the hand for a long time in, there is the danger of being frostbitten.

Therefore, there is a need for an apparatus for preserving umbilical cord mesenchymal stem cells to solve the above-mentioned problems.

Disclosure of Invention

Aiming at the situation, in order to overcome the defects of the prior art, the invention provides the device for preserving the umbilical cord mesenchymal stem cells, the access of the cryopreserved stem cell cryopreserving tube is automatically controlled, the convenient operation of the device is greatly increased, the exposed caliber of liquid nitrogen contacting with the outside is reduced, the loss of the liquid nitrogen is reduced, and meanwhile, the danger of hand frostbite of workers is reduced by automatically controlling the process of contacting with the liquid nitrogen in the whole access process.

The technical scheme adopted by the invention is as follows: the invention provides a device for storing umbilical cord mesenchymal stem cells, which comprises a storage shell, a cryopreservation placing unit, a storing and taking transmission mechanism, a cryopreservation buffer placing rack, a shielding compaction sealing mechanism and a magnetic automatic storing and taking mechanism, wherein the storage shell is a hollow cavity with an opening on the upper side, the cryopreservation placing unit is arranged in the storage shell, the storing and taking transmission mechanism is arranged outside the cryopreservation placing unit, the cryopreservation buffer placing rack is radially arranged on the cryopreservation placing unit, the shielding compaction sealing mechanism is arranged in the storage shell, the shielding compaction sealing mechanism is arranged at the upper part of the cryopreservation placing unit, and the magnetic automatic storing and taking mechanism is arrayed on the outer wall of the storage shell.

Preferably, the unit is placed to the cryopreserving includes the cryopreserving jar body, cryopreserving places dish, middle part support column, swivel support shaft and cryopreserving and places the hole, the cryopreserving jar body is upper end open-ended cavity setting, the middle part support column is located cryopreserving jar internal diapire middle part, the cryopreserving is placed the dish and is located middle part support column upper end, cryopreserving places the dish and locates cryopreserving jar body upside opening part, and the liquid nitrogen is deposited in the cryopreserving jar internally, and is deposited the dish lower part in cryopreserving, the cryopreserving is placed the hole and is radially run through and locate cryopreserving and place a set upper wall, and the cryopreserving buffering rack is placed in the cryopreserving and places downtheholely one by one, makes the dry cell cryopreserving pipe be in the liquid nitrogen and cryopreserves, the swivel support shaft runs through middle part support column, cryopreserving and places a set upper wall and cryopreserving jar body diapire, the swivel support shaft lower extreme is located and is deposited shell internal diapire center department, the cryopreserving is placed a set upper wall and is equipped with liquid ammonia inlet, is convenient for detecting the liquid ammonia liquid level in the cryopreserving jar internally, is convenient for in time supply liquid nitrogen simultaneously.

In order to facilitate the regulation to freeze and deposit jar body and rotate, and be convenient for automatic control freezes to deposit the through-hole alignment of getting on buffering rack and the compaction apron, access drive mechanism includes access transmission worm wheel, access rotating electrical machines and access transmission worm, access transmission worm wheel is located freezes and deposits jar external wall, access transmission worm is located and is deposited between the casing inside wall, access transmission worm one end is run through and is deposited casing one side lateral wall, access transmission worm and access transmission worm wheel meshing, access rotating electrical machines locates and deposits the casing outer wall, access rotating electrical machines is connected with access transmission worm.

Preferably, the cryopreservation buffer rack comprises a connecting block, a sealing baffle, a connecting rod, a cryopreservation placing plate, a cryopreservation tube placing hole, a buffer spring, a magnetic attraction layer and a buffer block, wherein the sealing baffle is arranged at the cryopreservation placing hole on the cryopreservation placing plate, the connecting block is arranged at the lower wall of the cryopreservation placing plate, the connecting block is arranged in the cryopreservation placing hole, the connecting rod array is arranged at the lower wall of the connecting block, the cryopreservation placing plate array is arranged on the connecting rod, the cryopreservation tube placing hole array is arranged on the cryopreservation placing plate, the upper wall of the sealing baffle is provided with a buffer groove, the buffer spring is arranged at the inner bottom wall of the buffer groove, the buffer block is arranged in the buffer groove, the buffer block is arranged at the upper end of the buffer spring, the magnetic attraction layer is arranged at the upper wall of the sealing baffle, the magnetic attraction layer is upwards adsorbed on the magnetic attraction layer of the fetching electromagnet, the sealing baffle is driven to upwards move, and the buffer block plays a buffer protection role in freezing and preserving the dry cell on the cryopreservation tube under the effect of the buffer spring.

Preferably, the shielding compaction sealing mechanism comprises a compaction cover plate, a sliding column, a supporting spring, a compaction threaded column, a compaction sleeve and a compaction handle, the sliding column is arranged on the upper end face of the rotating supporting shaft, a threaded hole is formed in the center of the upper end face of the sliding column, the supporting spring is arranged on the upper end face of the rotating supporting shaft, the supporting spring is sleeved on the sliding column, the compaction cover plate is arranged in the storage shell and is arranged on the sliding column in a sliding mode, the compaction cover plate is arranged on the upper end of the supporting spring and is arranged in a threaded hole in the upper end face of the sliding column, the compaction sleeve is arranged in a hollow cavity with one open end, the inner bottom wall of the compaction sleeve is arranged on the upper end face of the compaction threaded column, the compaction sleeve is arranged on the upper wall of the compaction cover plate, the compaction handle array is arranged on the upper portion of the outer wall of the compaction sleeve, a fetching through hole is arranged in a penetrating mode through the array, the fetching through hole corresponds to the freezing storage placing hole, the compaction sleeve is rotated, the compaction sleeve drives the compaction threaded column to ascend or descend, so that the compaction sleeve can adjust the height of the compaction cover plate, and the sealing baffle plate can be placed on the freezing storage hole to achieve the sealing effect of sealing when freezing storage.

In order to avoid the staff to stretch into the internal stem cell cryopreserving pipe of taking of cryopreserving jar with the hand, spacing frame array is located and is deposited upper portion in the casing lateral wall, spacing frame upper wall is equipped with the standing groove, the access lifter is the setting of L type, the access lifter slides and locates in the spacing frame, the access lifter lateral wall is located to the lifting rack, the lifting rack runs through the standing groove, spacing frame upper wall is located to the mount frame, the lifting drive shaft is located between the mount frame inside wall, mounting bracket one side lateral wall is run through to lifting drive shaft one end, lifting gear locates on the lifting drive shaft, lifting gear locates in the mount frame, lifting drive motor locates the mounting bracket lateral wall, lifting drive motor is connected with the lifting drive shaft, lifting gear and lifting rack meshing, access lifter one end is located to the access electromagnet, receives infrared receiver's signal, control access electromagnet inhales with the layer mutually, realizes automatically that taking of stem cell cryopreserving pipe.

The side wall array of the freezing tank body is provided with infrared emitters, the infrared emitters are aligned with the freezing storage placing holes, and the infrared emitters at specific positions need to be taken transmit signals.

Further, deposit the casing inside wall middle part and be equipped with infrared receiver, infrared receiver and infrared transmitter are in same vertical plane, and after infrared transmitter transmission signal, when rotating to infrared receiver department, infrared receiver receives the signal that infrared transmitter sent, with signal transmission to the controller.

In order to facilitate the automatic usefulness of getting of realizing stem cell cryopreserving pipe, it is equipped with the controller to deposit the casing lateral wall, it is equipped with control panel to deposit the casing lateral wall, and control panel can input the specific position that looks over and take the stem cell cryopreserving pipe that needs, the controller is connected with control panel electricity, the controller is connected with the infrared receiver communication, the controller is connected with the infrared transmitter communication, the controller is connected with the access rotating electrical machines electricity, the controller is connected with the lift drive motor electricity, the controller is connected with the access electro-magnet electricity.

In the device, the lower part of the storage shell is symmetrically provided with two mobile supporting legs, and the lower ends of the mobile supporting legs are provided with universal wheels, so that the device can be conveniently and flexibly moved.

The invention with the structure has the following beneficial effects:

1. in the freezing storage placing unit, the freezing storage placing holes are radially arranged, the freezing storage placing holes on each radiation radius correspond to one freezing storage buffer placing frame, each radiation radius corresponds to one infrared transmitter, a transmitting signal of each infrared transmitter is received by each infrared receiver, the freezing storage buffer placing frames needing to be taken out and stored can be quickly judged, only one freezing storage placing hole is opened when the liquid nitrogen is taken out and stored every time, the caliber size of the liquid nitrogen contacting with the outside is reduced, and further the loss of the liquid nitrogen is reduced;

2. in the magnetic automatic storing and taking mechanism, the magnetic automatic storing and taking mechanism corresponds to the cryopreservation buffer rack one by one, the position of the cryopreservation buffer rack to be finally searched is determined by utilizing a control panel, the storing and taking electromagnet is automatically opened to adsorb the cryopreservation buffer rack, and the upper end of the cryopreservation buffer rack is provided with a buffer block;

3. in the shielding compaction sealing mechanism, each freezing buffer placing frame is independent, so that the freezing buffer placing frames are not tightly connected for quick fetching and storing, and a compaction threaded column is sleeved in the middle of a compaction sleeve, so that the sealing problem of all the freezing buffer placing frames is solved, the loss of liquid nitrogen is reduced, the stability is improved, and the protection effect is achieved;

4. in this equipment, all adopt automatic the completion with the process of the contact of liquid nitrogen in the access of tube is deposited to stem cell freezing, avoided the danger that the staff was frostbitten completely, reduced the loss of liquid nitrogen simultaneously.

Drawings

FIG. 1 is a schematic diagram of the internal three-dimensional structure of a device for preserving umbilical cord mesenchymal stem cells according to the present invention;

FIG. 2 is a schematic external perspective view of an apparatus for preserving umbilical cord mesenchymal stem cells according to the present invention;

FIG. 3 is a top view of the internal structure of an apparatus for preserving umbilical cord mesenchymal stem cells according to the present invention;

FIG. 4 is a schematic diagram of the internal three-dimensional structure of the cryopreservation unit of the device for preserving umbilical cord mesenchymal stem cells according to the present invention;

FIG. 5 is a schematic perspective view of a magnetic automatic accessing mechanism of a device for storing umbilical cord mesenchymal stem cells according to the present invention;

FIG. 6 is an enlarged view of portion A of FIG. 5;

FIG. 7 is an enlarged view of portion B of FIG. 4;

FIG. 8 is a schematic perspective view of a compaction sleeve and a compaction threaded column of the device for preserving umbilical cord mesenchymal stem cells according to the present invention;

FIG. 9 is a schematic perspective view of a compact cover plate of the device for preserving umbilical cord mesenchymal stem cells according to the present invention;

fig. 10 is an exploded perspective view of a cryopreservation buffer rack of a magnetic automatic storing and taking mechanism of a device for storing umbilical cord mesenchymal stem cells according to the present invention.

Wherein, 1, deposit the casing, 2, freeze and deposit and place the unit, 3, deposit and take out drive mechanism, 4, freeze and deposit the buffering rack, 5, shelter from compaction sealing mechanism, 6, magnetism inhale automatic deposit and take out mechanism, 7, freeze and deposit the jar body, 8, freeze and deposit and place the dish, 9, middle part support column, 10, swivel support shaft, 11, freeze and deposit and place the hole, 12, deposit and take out the transmission worm wheel, 13, deposit and take out the rotating electrical machines, 14, deposit and take out the transmission worm, 15, connecting block, 16, seal baffle, 17, connecting rod, 18, freeze and deposit the board, 19, freeze and deposit the pipe and place the hole, 20, buffer spring, 21, buffer block, 22, a buffer groove, 23, a compaction cover plate, 24, a sliding column, 25, a support spring, 26, a compaction threaded column, 27, a compaction sleeve, 28, a compaction handle, 29, a fetching through hole, 30, a limit frame, 31, a mounting frame, 32, a lifting gear, 33, a lifting transmission shaft, 34, a lifting transmission motor, 35, a fetching lifting rod, 36, a lifting rack, 37, a fetching electromagnet, 38, a placing groove, 39, an infrared emitter, 40, an infrared receiver, 41, a controller, 42, a movable supporting leg, 43, a universal wheel, 44 and a liquid ammonia inlet, 45, a control panel, 46 and a magnetic absorption layer.

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus should not be construed as limiting the present invention.

As shown in the figures 1 and 2, the invention provides a device for preserving umbilical cord mesenchymal stem cells, which comprises a storage shell 1, a cryopreservation placing unit 2, a storing and taking transmission mechanism 3, a cryopreservation buffer placing rack 4, a shielding compaction sealing mechanism 5 and a magnetic automatic storing and taking mechanism 6, wherein the storage shell 1 is provided with a hollow cavity with an open upper end, the cryopreservation placing unit 2 is arranged in the storage shell 1, the storing and taking transmission mechanism 3 is arranged outside the cryopreservation placing unit 2, the cryopreservation buffer placing rack 4 is radially arranged on the cryopreservation placing unit 2, the shielding compaction sealing mechanism 5 is arranged in the storage shell 1, the shielding compaction sealing mechanism 5 is arranged on the upper part of the cryopreservation placing unit 2, and the magnetic automatic storing and taking mechanism 6 is arranged on the outer wall of the storage shell 1 in an array manner.

As shown in fig. 1, fig. 3, fig. 4 shows, the unit 2 is placed including freezing the jar body 7 of depositing, freezing and depositing places dish 8, middle part support column 9, swivel support shaft 10 and freezing and deposit and place hole 11, freezing the jar body 7 of depositing is the cavity setting of upper side open-ended, middle part support column 9 locates freezing jar body 7 inner bottom wall middle part of depositing, freezing and depositing places dish 8 and locates middle part support column 9 upper end, freezing and depositing places dish 8 and locates freezing jar body 7 upside opening part, liquid nitrogen is deposited in freezing and deposits jar body 7, and deposit and place the dish 8 lower part in freezing, freezing and depositing hole 11 is radial liquid ammonia and runs through and locates freezing and places the dish 8 upper wall, freezing and depositing buffer rack 4 places in freezing and places hole 11, make dry cell freezing and deposit the pipe in well freezing and deposit, swivel support shaft 10 runs through liquid nitrogen middle part support column 9, freezing and depositing dish 8 upper wall and freezing and depositing jar body 7 diapire, swivel support shaft 10 lower extreme is located and deposits casing 1 inner bottom wall center department, freezing and depositing the dish 8 upper wall is equipped with liquid ammonia inlet 44, be convenient for detecting the liquid nitrogen, in time, the replenishment of liquid nitrogen of being convenient for simultaneously.

As shown in fig. 1, fig. 3 and fig. 4, in order to facilitate the adjustment of the rotation of the freezing storage tank body 7, and in order to facilitate the automatic control of the alignment of the freezing storage buffer rack 4 and the access through hole 29 on the compaction cover plate 23, the access transmission mechanism 3 comprises an access transmission worm wheel 12, an access rotating motor 13 and an access transmission worm 14, the access transmission worm wheel 12 is arranged on the outer wall of the freezing storage tank body 7, the access transmission worm 14 is arranged between the inner side walls of the storage shell 1, one end of the access transmission worm 14 penetrates through the side wall of one side of the storage shell 1, the access transmission worm 14 is meshed with the access transmission worm wheel 12, the access rotating motor 13 is arranged on the outer wall of the storage shell 1, and the access rotating motor 13 is connected with the access transmission worm 14.

As shown in fig. 1, fig. 3, and fig. 10, the cryopreservation buffer rack 4 includes a connection block 15, a sealing baffle 16, a connection rod 17, a cryopreservation placing plate 18, a cryopreservation tube placing hole 19, a buffer spring 20, a magnetic absorption layer 46 and a buffer block 21, the sealing baffle 16 is disposed at the cryopreservation placing hole 11 on the cryopreservation placing plate 8, the connection block 15 is disposed at the lower wall of the cryopreservation placing plate 8, the connection block 15 is disposed in the cryopreservation placing hole 11, the connection rod 17 is disposed at the lower wall of the connection block 15 in an array manner, the cryopreservation placing plate 18 is disposed on the connection rod 17 in an array manner, the cryopreservation tube placing hole 19 is disposed on the cryopreservation placing plate 18 in an array manner so as to store standard cryopreservation tubes on the market, the cryopreservation tube placing plate 18 and the cryopreservation tube placing hole 19 increase the storage number of the cryopreservation tubes, the buffer groove 22 is disposed on tubes on the upper wall of the sealing baffle 16, the buffer block 21 is disposed in the buffer groove 22, the buffer block 21 is disposed at the upper end of the buffer spring 20, the buffer layer 46 is disposed on the upper wall of the sealing baffle 16, the buffer baffle 16 absorbs the magnetic absorption layer 46, and drives the buffer baffle 16 to absorb the magnetic absorption buffer block 20 to protect the buffer block 20 and the buffer block 21 to protect the cryopreservation tube.

As shown in fig. 2, 4, 7, 8, the shielding compaction and sealing mechanism 5 includes a compaction cover plate 23, a sliding column 24, a supporting spring 25, a compaction threaded column 26, a compaction sleeve 27, and a compaction handle 28, the sliding column 24 is disposed on the upper end surface of the rotating support shaft 10, a threaded hole is disposed at the center of the upper end surface of the sliding column 24, the supporting spring 25 is disposed on the upper end surface of the rotating support shaft 10, the supporting spring 25 is sleeved on the sliding column 24, the compaction cover plate 23 is disposed in the storage housing 1, the compaction cover plate 23 is slidably disposed on the sliding column 24, the compaction cover plate 23 is disposed on the upper end of the supporting spring 25, the compaction threaded column 26 is disposed in a threaded hole on the upper end surface of the sliding column 24, the compaction sleeve 27 is disposed in a hollow cavity with an opening at one end, the inner bottom wall of the compaction sleeve 27 is disposed on the upper end surface of the compaction threaded column 26, the compaction sleeve 27 is disposed on the upper wall of the compaction cover plate 23, the compaction handle 28 is disposed on the upper wall of the compaction sleeve 27, a fetching and storing through hole 29 is disposed on the upper wall of the compaction cover plate 23, the fetching through hole 29 is disposed corresponding to the freezing storing hole, the freezing storage sleeve 27 is rotated, the compaction sleeve 27 is rotated to realize the lifting and the sealing of the compaction cover plate 27, thereby the sealing mechanism can be used for adjusting the height of the freezing storage plate 16, and sealing baffle plate 11.

As shown in fig. 1, 2, 3, 5, 6, in order to avoid the worker from extending his/her hand into the cryopreservation tank 7 to take the stem cell cryopreservation tube, the magnetic automatic storing and taking mechanism 6 includes a limiting frame 30, a mounting frame 31, a lifting gear 32, a lifting transmission shaft 33, a lifting transmission motor 34, a storing and taking lifting rod 35, a lifting rack 36 and a storing and taking electromagnet 37, the limiting frame 30 is arranged on the middle upper portion of the outer side wall of the storage shell 1, a placing groove 38 is arranged on the upper wall of the limiting frame 30, the storing and taking lifting rod 35 is arranged in an L shape, the storing and taking lifting rod 35 is slidably arranged in the limiting frame 30, the lifting rack 36 is arranged on the side wall of the storing and taking lifting rod 35, the lifting rack 36 penetrates through the placing groove 38, the mounting frame 31 is arranged on the upper wall of the limiting frame 30, the lifting transmission shaft 33 is arranged between the inner side wall of the mounting frame 31, one end of the lifting transmission shaft 33 penetrates through the side wall of the mounting frame 31, the lifting gear 32 is arranged on the lifting transmission shaft 33, the lifting gear 32 is arranged in the mounting frame 31, the outer side wall of the mounting frame 31, the lifting transmission motor 34 is arranged on the lifting transmission shaft 34, the lifting transmission shaft 34 is connected with the lifting transmission shaft 33, the lifting gear 32, one end of the lifting gear 32 is arranged to receive a magnetic automatic storing and taking electromagnet of the infrared receiver 40, and the infrared receiver for realizing the infrared receiver for controlling the infrared receiver 46 for controlling the automatic storing and taking of the freezing cell taking electromagnet for realizing the infrared receiver, and taking.

As shown in fig. 1, 3 and 4, the sidewall array of the cryopreservation tank 7 is provided with infrared emitters 39, the infrared emitters 39 are aligned with the cryopreservation placing holes 11, and the infrared emitters 39 at specific positions to be taken emit signals.

As shown in fig. 1, 3 and 4, an infrared receiver 40 is disposed in the middle of the inner side wall of the storage case 1, the infrared receiver 40 and the infrared transmitter 39 are in the same vertical plane, and when the infrared transmitter 39 transmits a signal and rotates to the infrared receiver 40, the infrared receiver 40 receives the signal transmitted by the infrared transmitter 39 and transmits the signal to the controller 41.

As shown in fig. 1, 3, 5 and 6, in order to facilitate the automatic taking of the stem cell cryopreservation tube, a controller 41 is arranged on the outer side wall of the storage shell 1, a control panel 45 is arranged on the outer side wall of the storage shell 1, the control panel 45 can input the specific position of the stem cell cryopreservation tube to be checked and taken as required, the controller 41 is electrically connected with the control panel 45, the controller 41 is in communication connection with an infrared receiver 40, the controller 41 is in communication connection with an infrared emitter 39, the controller 41 is electrically connected with the access rotating motor 13, the controller 41 is electrically connected with the lifting transmission motor 34, and the controller 41 is electrically connected with the access electromagnet 37.

As shown in figure 1, the lower part of the storage shell 1 is provided with two mobile supporting legs 42 which are symmetrical, and the lower ends of the mobile supporting legs 42 are provided with universal wheels 43, so that the device can move flexibly.

In the first embodiment, the device is moved to a corresponding storage place, when the frozen pipes are stored, firstly, the compaction handle 28 is rotated, the compaction sleeve 27 is rotated, the compaction threaded column 26 is driven by the compaction sleeve 27 to rotate, the compaction threaded column 26 moves upwards in the threaded hole, at the moment, the support spring 25 upwards bounces the compaction cover plate 23, the compaction cover plate 23 is separated from the buffer block 21, then the access rotary motor 13 is controlled to rotate, the access rotary motor 13 drives the frozen tank body 7 to rotate, the frozen buffer storage rack 4 is staggered with the access through hole 29, then the compaction handle 28 is rotated in a reverse rotation mode, the compaction sleeve 27 drives the compaction threaded column 26 to rotate, the compaction threaded shaft moves downwards, the compaction sleeve 27 presses the compaction cover plate 23 downwards until the compaction cover plate 23 compresses the buffer block 21 and the sealing baffle plate 16, and the sealing baffle plate 16 plays a sealing role;

in the second embodiment, when the frozen stem cells are required to be taken out, the freezing tube is firstly rotated to compact the handle 28, the compaction sleeve 27 is rotated, the compaction sleeve 27 drives the compaction threaded column 26 to rotate, the compaction threaded column 26 moves upwards in the threaded hole, at the moment, the support spring 25 upwards bounces the compaction cover plate 23, the compaction cover plate 23 is separated from the buffer block 21, then the infrared emitter 39 arranged on the corresponding radial cryopreservation buffer rack 4 on the control panel 45 is used for starting emission, at the moment, the controller 41 turns on the access rotating motor 13, the access rotating motor 13 drives the access transmission worm 14 to rotate, the access transmission worm 14 drives the access transmission worm wheel 12 to rotate, the access transmission worm wheel 12 drives the cryopreservation tank body 7 to rotate, when the infrared receiver 40 receives the signal sent by the infrared transmitter 39, the infrared receiver 40 transmits the signal to the controller 41, the controller 41 controls the access rotating motor 13 to stop, and at this moment, the controller 41 opens the corresponding access electromagnet 37, the access electromagnet 37 upwards adsorbs the magnetic adsorption layer 46, the sealing baffle 16 is upwards adsorbed, at this moment, the buffer block 21 firstly contacts the access electromagnet 37 under the action of the buffer spring 20, the buffer block 21 plays a role in buffering and protecting the freezing pipes on the freezing placing plate 18, so as to avoid damage, then the controller 41 opens the corresponding lifting transmission motor 34, the lifting transmission motor 34 drives the lifting transmission shaft 33 to rotate, the lifting transmission shaft 33 drives the lifting gear 32 to rotate, the lifting gear 32 drives the lifting rack 36 to move upwards, the lifting rack 36 drives the storing and taking lifting rod 35 to move upwards, the storing and taking electromagnet 37 adsorbs the sealing baffle 16 to drive the connecting block 15 and the cryopreservation tube on the cryopreservation placing plate 18 to ascend, and at the moment, a worker can conveniently take out the required stem cell cryopreservation tube;

third embodiment, when the stem cell cryopreserving pipe needs to be stored, the control panel 45 can be used for checking the corresponding position of the cryopreserving placing plate 18 where the cryopreserving pipe is not placed, the operation of the first embodiment is repeated, the worker places the stem cell cryopreserving pipe in the cryopreserving pipe placing hole 19, the control panel 45 is operated, the controller 41 controls the lifting transmission motor 34 to rotate reversely, the lifting transmission motor 34 drives the lifting gear 32 to rotate reversely, the lifting gear 32 drives the lifting rack 36 to move downwards, the access lifting rod 35 drives the access electromagnet 37 to adsorb the stem cell cryopreserving pipe on the cryopreserving plate 18 and descend to the cryopreserving placing hole 11, and then the controller 41 controls the access electromagnet 37 to be powered off, so that the storage of the stem cell cryopreserving pipe is completed.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

The present invention and its embodiments have been described above, and the description is not intended to be limiting, and the drawings are only one embodiment of the present invention, and the actual structure is not limited thereto. In summary, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (5)

1. An apparatus for preserving umbilical cord mesenchymal stem cells, characterized in that: comprises that

The storage device comprises a storage shell (1), wherein the storage shell (1) is a hollow cavity with an opening at the upper side;

the freezing storage unit (2), the freezing storage unit (2) is arranged in the storage shell (1);

the access transmission mechanism (3) is arranged in the storage shell (1), and the access transmission mechanism (3) is arranged outside the cryopreservation unit (2);

the freezing and storing buffer placing frame (4) is radially arranged on the freezing and storing unit (2);

the shielding compaction sealing mechanism (5) is arranged in the storage shell (1), and the shielding compaction sealing mechanism (5) is arranged at the upper part of the freezing storage unit (2);

the magnetic automatic storing and taking mechanism (6) is arranged on the outer wall of the storage shell (1) in an array manner;

the freezing storage placing unit (2) comprises a freezing storage tank body (7), a freezing storage placing disc (8), a middle supporting column (9), a rotating supporting shaft (10) and a freezing storage placing hole (11), wherein the freezing storage tank body (7) is provided with a hollow cavity with an open upper side, the middle supporting column (9) is arranged in the middle of the inner bottom wall of the freezing storage tank body (7), the freezing storage placing disc (8) is arranged at the upper end of the middle supporting column (9), the freezing storage placing disc (8) is arranged at an upper side opening of the freezing storage tank body (7), the freezing storage placing hole (11) radially penetrates through the upper wall of the freezing storage placing disc (8), the rotating supporting shaft (10) penetrates through the middle supporting column (9), the upper wall of the freezing storage placing disc (8) and the bottom wall of the freezing storage tank body (7), the lower end of the rotating supporting shaft (10) is arranged at the center of the inner bottom wall of the storage shell (1), and a liquid ammonia adding port (44) is arranged on the upper wall of the freezing storage placing disc (8);

the access transmission mechanism (3) comprises an access transmission worm wheel (12), an access rotating motor (13) and an access transmission worm (14), the access transmission worm wheel (12) is arranged on the outer wall of the cryopreservation tank body (7), the access transmission worm (14) is arranged between the inner side walls of the storage shell (1), one end of the access transmission worm (14) penetrates through the side wall of one side of the storage shell (1), the access transmission worm (14) is meshed with the access transmission worm wheel (12), the access rotating motor (13) is arranged on the outer wall of the storage shell (1), and the access rotating motor (13) is connected with the access transmission worm (14);

the cryopreservation buffer placing frame (4) comprises a connecting block (15), a sealing baffle (16), a connecting rod (17), a cryopreservation placing plate (18), a cryopreservation tube placing hole (19), a buffer spring (20), a magnetic attraction layer (46) and a buffer block (21), wherein the sealing baffle (16) is arranged at the cryopreservation placing hole (11) on the cryopreservation placing plate (8), the connecting block (15) is arranged at the lower wall of the cryopreservation placing plate (8), the connecting block (15) is arranged in the cryopreservation placing hole (11), the connecting rod (17) is arranged at the lower wall of the connecting block (15) in an array manner, the cryopreservation placing plate (18) is arranged at the lower wall of the connecting block (17), the cryopreservation tube placing hole (19) is arranged at the cryopreservation placing plate (18), the upper wall of the sealing baffle (16) is provided with the buffer groove (22), the buffer spring (20) is arranged at the inner bottom wall of the buffer groove (22), the buffer block (21) is arranged in the buffer groove (22), the upper end of the buffer spring (20) is arranged at the upper end of the magnetic attraction layer (46);

the shielding compaction sealing mechanism (5) comprises a compaction cover plate (23), a sliding column (24), a supporting spring (25), a compaction thread column (26), a compaction sleeve (27) and a compaction handle (28), the sliding column (24) is arranged on the upper end surface of the rotating support shaft (10), a threaded hole is arranged at the center of the upper end surface of the sliding column (24), the supporting spring (25) is arranged on the upper end surface of the rotary supporting shaft (10), the supporting spring (25) is sleeved on the sliding column (24), the compaction cover plate (23) is arranged in the storage shell (1), the compaction cover plate (23) is arranged on the sliding column (24) in a sliding way, the compaction cover plate (23) is arranged at the upper end of the supporting spring (25), the compaction threaded column (26) is arranged in a threaded hole on the upper end surface of the sliding column (24), the compaction sleeve (27) is arranged in a hollow cavity with one open end, the inner bottom wall of the compaction sleeve (27) is arranged on the upper end surface of the compaction threaded column (26), the compaction sleeve (27) is arranged on the upper wall of the compaction cover plate (23), the compaction handles (28) are arranged at the upper part of the outer wall of the compaction sleeve (27) in an array, the upper wall of the compaction cover plate (23) is provided with access through holes (29) in a penetrating array, the access through hole (29) is arranged corresponding to the freezing storage placing hole (11);

the magnetic attraction automatic access mechanism (6) comprises a limiting frame (30), an installation frame (31), a lifting gear (32), a lifting transmission shaft (33), a lifting transmission motor (34), an access lifting rod (35), a lifting rack (36) and an access electromagnet (37), wherein the limiting frame (30) is arranged on the middle upper part of the outer side wall of the storage shell (1) in an array manner, a placing groove (38) is formed in the upper wall of the limiting frame (30), the access lifting rod (35) is arranged in an L-shaped manner, the access lifting rod (35) is arranged in the limiting frame (30) in a sliding manner, the lifting rack (36) is arranged on the side wall of the access lifting rod (35), the lifting rack (36) penetrates through the placing groove (38), the installation frame (31) is arranged on the upper wall of the limiting frame (30), the lifting transmission shaft (33) is arranged between the inner side walls of the installation frame (31), one end of the lifting transmission shaft (33) penetrates through the side wall of the installation frame (31), the lifting gear (32) is arranged on the lifting transmission shaft (33), the lifting gear (32) is arranged in the lifting gear (32) arranged in the installation frame (31), the outer side wall of the lifting transmission motor (34), the outer side wall of the lifting transmission shaft (31) is connected with the lifting gear (34), the lifting gear (32) and the lifting gear (32) is meshed with the lifting gear (32), the access electromagnet (37) is arranged at one end of the access lifting rod (35).

2. The device for preserving umbilical cord mesenchymal stem cells according to claim 1, wherein: the side wall array of the freezing tank body (7) is provided with infrared emitters (39), and the infrared emitters (39) are aligned with the freezing storage placing holes (11).

3. An apparatus for preserving umbilical cord mesenchymal stem cells according to claim 2, wherein: deposit casing (1) inside wall middle part and be equipped with infrared receiver (40), infrared receiver (40) and infrared emitter (39) are in same vertical plane.

4. An apparatus for preserving umbilical cord mesenchymal stem cells according to claim 3, wherein: deposit casing (1) lateral wall and be equipped with controller (41), it is equipped with control panel (45) to deposit casing (1) lateral wall, controller (41) are connected with control panel (45) electricity, controller (41) are connected with infrared receiver (40) communication, controller (41) are connected with infrared emitter (39) communication, controller (41) are connected with access rotating electrical machines (13) electricity, controller (41) are connected with lift drive motor (34) electricity, controller (41) are connected with access electro-magnet (37) electricity.

5. An apparatus for preserving umbilical cord mesenchymal stem cells according to claim 4, wherein: the storage shell (1) is characterized in that the lower part of the storage shell (1) is provided with movable supporting legs (42) in pairwise symmetry, and the lower ends of the movable supporting legs (42) are provided with universal wheels (43).

Images