CN114886932A - Ultralow-temperature wall breaking method for ganoderma lucidum spore powder - Google Patents
Ultralow-temperature wall breaking method for ganoderma lucidum spore powder Download PDFInfo
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Abstract
The invention relates to the technical field of low-temperature wall breaking, and particularly discloses an ultralow-temperature wall breaking method for ganoderma lucidum spore powder, which comprises the following steps: and (3) putting the frozen product into an ultrasonic wall breaking machine for wall breaking, obtaining a wall breaking material after wall breaking, and then washing and drying the wall breaking material. The ganoderma lucidum spore powder is subjected to ultrasonic dispersion treatment by adopting the protective wall soaking solution, and then is radiated by high-energy electron beams, so that the effective wall breaking treatment is carried out on the outer wall of the ganoderma lucidum spore powder, the wall breaking rate is improved, the protective wall soaking solution is matched with the wall soaking main agent solution and the modified montmorillonite, the content of the ganoderma lucidum spore powder can be effectively coated, the nutritional wall breaking components are effectively protected while the wall is broken, and the wall breaking efficiency is improved by further combining ball milling treatment and low-temperature wall breaking; during wall breaking, tremella extract is also adopted to be stirred and mixed, wall breaking components are further protected, and therefore the nutritional ingredients of wall breaking products are protected under the condition of improving the wall breaking efficiency.
Description
Technical Field
The invention relates to the technical field of wall breaking of ganoderma lucidum spore powder, and particularly relates to an ultralow-temperature wall breaking method of ganoderma lucidum spore powder.
Background
Ganoderma spore is the seeds of Ganoderma lucidum which is the tiny egg-shaped germ cells ejected from Ganoderma lucidum fold during the growth and maturation period. Each ganoderma spore is only 4-6 microns, is a living organism, has a double-wall structure, is surrounded by hard chitin cellulose, and is difficult to be fully absorbed by a human body. After the wall is broken, the medicine is more suitable for direct absorption of intestines and stomach of human body. The Ganoderma spore powder has all genetic materials of Ganoderma and health promotion effect. The medicinal value of the ganoderma lucidum spore is increasingly valued, and researches show that the ganoderma lucidum spore has the effects of enhancing the immunity of organisms, inhibiting tumors, protecting liver injury and protecting radiation.
The existing ganoderma lucidum spore powder is subjected to wall breaking treatment, so that the wall breaking rate is improved, the nutrient components are easily damaged, the nutrient value of the ganoderma lucidum spore powder subjected to wall breaking is reduced, and the use of the product is limited.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide an ultralow-temperature wall breaking method for ganoderma lucidum spore powder, so as to solve the problems in the background art.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the invention provides an ultralow-temperature wall breaking method for ganoderma lucidum spore powder, which comprises the following steps:
the method comprises the following steps: adding Ganoderma spore powder into 3-5 times of the wall soaking solution, performing ultrasonic dispersion treatment at 45-65 deg.C, washing with water, and drying;
step two: then sending the product obtained in the step one into a high-energy electron beam for radiation treatment, and obtaining a material to be subjected to wall breaking after the treatment is finished;
step three: sending the wall-broken material into a ball mill for ball milling treatment, and obtaining ball grinding materials after ball milling is finished;
step four: fully stirring and mixing the ball grinding material and the tremella extract with the volume of 3-5 times of the tremella extract, and then freezing for 2-3h at-35 ℃;
step five: and then putting the frozen product into an ultrasonic wall breaking machine for wall breaking, obtaining a wall breaking material after wall breaking, and then washing and drying the wall breaking material.
Preferably, the power of the ultrasonic dispersion treatment in the first step is 300-.
The inventor finds that the free radical clearance rate of the product is improved without adopting high-energy electron beam treatment, and finds that the white fungus extract treatment has synergistic effect and can be matched with the protective wall soaking liquid to enhance the free radical clearance rate effect.
Preferably, the preparation method of the coating wall-soaking solution comprises the following steps:
s01: adding 5-10 parts of chitosan into 20-25 parts of water, then adding acetic acid, adjusting the pH value to 5.0-6.0, then adding 2-5 parts of sodium alginate, and stirring and fully dispersing to obtain a wall-soaking main agent solution;
s02: the montmorillonite is sent into a thermal reaction furnace for reaction for 20-30min, the reaction is finished, then ball milling is carried out for 10-20min at the speed of 800-;
s03: adding the modified montmorillonite into 2-4 times of the wall-soaking main agent solution, stirring at 55-65 ℃ for 20-40min, stirring at 450-550r/min, and obtaining the protective wall-soaking solution after the stirring is finished.
Preferably, the rotation speed of the S01 for sufficient stirring and dispersion is 1000-1500r/min, and the stirring time is 20-30 min.
The inventor of the invention finds that the DPPH free radical clearance and ABTS free radical clearance of the product are obviously reduced without adding the protective wall-soaking liquid, meanwhile, the preparation methods of the protective wall-soaking liquid are different without adding modified montmorillonite, and the DPPH free radical clearance and ABTS free radical clearance are different, so that the protective wall-soaking liquid has the effects of obviously improving the DPPH free radical clearance and ABTS free radical clearance;
although the high-energy electron beam treatment can destroy the free radical clearance, the wall breaking rate and the ganoderan content of the product can be improved, the protective wall soaking liquid and the tremella extract are cooperatively used for compensating the damage of the free radical clearance, so that the free radical clearance of the product is improved, the high-energy electron beam treatment is matched with the protective wall soaking liquid and the tremella extract, the wall breaking rate of the product and the protection effect of the product components are coordinately improved, and the free radical clearance of the product is enhanced.
Preferably, the temperature of the thermal reaction furnace is 500-600 ℃.
Preferably, the high-energy electron beam radiation is Co gamma rays, and the radiation dose is 10-20 kGy.
Preferably, the ball milling rotation speed of the ball mill is 500-700r/min, the ball milling time is 15-25min, and the ball milling temperature is 5-10 ℃.
Preferably, the preparation method of the tremella extract comprises the following steps:
cleaning Tremella in water, draining, treating in 85-95 deg.C steam for 10-20min, extracting in 3-5 times of water, and collecting Tremella extractive solution.
Preferably, the temperature of the extraction treatment is 100 ℃, and the extraction time is 1-2 h.
Preferably, the temperature of the ultrasonic wall breaking machine is 45-55 deg.C, and the treatment time is 25-35 min.
Compared with the prior art, the invention has the following beneficial effects:
the ganoderma lucidum spore powder is subjected to ultrasonic dispersion treatment by adopting the protective wall soaking solution, and then is radiated by high-energy electron beams, so that the effective wall breaking treatment is carried out on the outer wall of the ganoderma lucidum spore powder, the wall breaking rate is improved, the protective wall soaking solution is matched with the wall soaking main agent solution and the modified montmorillonite, the content of the ganoderma lucidum spore powder can be effectively coated, the nutritional wall breaking components are effectively protected while the wall is broken, and the wall breaking efficiency is improved by further combining ball milling treatment and low-temperature wall breaking; still adopt white fungus extract stirring to mix in the broken wall, further protection broken wall composition to under the efficiency that improves the broken wall rate, the nutrient composition of protection broken wall product, the nutrient composition that obtains can carry out the free radical with the high efficiency and clear away.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides an ultralow-temperature wall breaking method for ganoderma lucidum spore powder, which comprises the following steps:
the method comprises the following steps: adding Ganoderma spore powder into 3-5 times of the wall soaking solution, performing ultrasonic dispersion treatment at 45-65 deg.C, washing with water, and drying;
step two: then sending the product obtained in the step one into a high-energy electron beam for radiation treatment, and obtaining a material to be subjected to wall breaking after the treatment is finished;
step three: sending the wall-broken material into a ball mill for ball milling treatment, and obtaining ball grinding materials after ball milling is finished;
step four: fully stirring and mixing the ball grinding material and the tremella extract with the volume of 3-5 times of the tremella extract, and then freezing for 2-3h at-35 ℃;
step five: and then putting the frozen product into an ultrasonic wall breaking machine for wall breaking, obtaining a wall breaking material after wall breaking, and then washing and drying the wall breaking material.
In the first step of this embodiment, the power of the ultrasonic dispersion treatment is 300-500W, and the ultrasonic time is 10-20 min.
The preparation method of the coating wall dipping solution of the embodiment comprises the following steps:
s01: adding 5-10 parts of chitosan into 20-25 parts of water, then adding acetic acid, adjusting the pH value to 5.0-6.0, then adding 2-5 parts of sodium alginate, and stirring and fully dispersing to obtain a wall-soaking main agent solution;
s02: the montmorillonite is sent into a thermal reaction furnace for reaction for 20-30min, the reaction is finished, then ball milling is carried out for 10-20min at the speed of 800-;
s03: adding the modified montmorillonite into 2-4 times of the wall-soaking main agent solution, stirring at 55-65 ℃ for 20-40min, stirring at 450-550r/min, and obtaining the protective wall-soaking solution after the stirring is finished.
In the embodiment, the rotation speed of the S01 for sufficient stirring and dispersion is 1000-1500r/min, and the stirring time is 20-30 min.
The temperature of the thermal reaction furnace in this embodiment is 500-600 ℃.
The high-energy electron beam radiation of the embodiment is Co gamma rays, and the radiation dose is 10-20 kGy.
The ball milling rotation speed of the ball mill of the embodiment is 500-.
The preparation method of the tremella extract in this embodiment includes:
cleaning Tremella in water, draining, treating in 85-95 deg.C steam for 10-20min, extracting in 3-5 times of water, and collecting Tremella extractive solution.
The temperature of the extraction treatment in this example was 100 deg.C, and the extraction time was 1-2 h.
Example 1.
The invention provides an ultralow-temperature wall breaking method for ganoderma lucidum spore powder, which comprises the following steps:
the method comprises the following steps: adding Ganoderma spore powder into 3 times of the wall soaking solution, performing ultrasonic dispersion treatment at 45 deg.C, washing with water, and drying;
step two: then sending the product obtained in the step one into a high-energy electron beam for radiation treatment, and obtaining a material to be subjected to wall breaking after the treatment is finished;
step three: sending the wall-broken material into a ball mill for ball milling treatment, and obtaining ball grinding materials after ball milling is finished;
step four: fully stirring and mixing the ball grinding material and the tremella extract with the volume of 3 times of the tremella extract, and then freezing for 2 hours at the temperature of minus 35 ℃;
step five: and then putting the frozen product into an ultrasonic wall breaking machine for wall breaking, obtaining a wall breaking material after wall breaking, and then washing and drying the wall breaking material.
In the first step of this embodiment, the power of the ultrasonic dispersion treatment is 300W, and the ultrasonic time is 10 min.
The preparation method of the coating wall-soaking solution in the embodiment comprises the following steps:
s01: adding 5 parts of chitosan into 20 parts of water, then adding acetic acid, adjusting the pH value to 5.0, then adding 2 parts of sodium alginate, and stirring and fully dispersing to obtain a wall-soaking main agent solution;
s02: feeding montmorillonite into a thermal reaction furnace for reaction for 20min, finishing the reaction, then performing ball milling at 800r/min for 10min, and finishing the ball milling to obtain modified montmorillonite;
s03: adding the modified montmorillonite into 2 times of the wall-soaking main agent solution, stirring at 55 ℃ for 20min, stirring at 450r/min, and obtaining the protective wall-soaking solution after the stirring is finished.
In the present embodiment, the rotation speed of the S01 for sufficient stirring and dispersion is 1000r/min, and the stirring time is 20 min.
The temperature of the thermal reaction furnace of this example was 500 ℃.
The high-energy electron beam radiation of this example was Co gamma rays with a radiation dose of 10 kGy.
The ball milling speed of the ball mill of the embodiment is 500r/min, the ball milling time is 15min, and the ball milling temperature is 5 ℃.
The preparation method of the tremella extract in this embodiment includes:
putting the tremella into water for cleaning, draining, putting into water vapor at 85 ℃ for processing for 10min, putting into 3 times of water for extraction, and obtaining the tremella extract after extraction.
The temperature of the extraction treatment in this example was 100 ℃ and the extraction time was 1 hour.
Example 2.
The invention provides an ultralow-temperature wall breaking method for ganoderma lucidum spore powder, which comprises the following steps:
the method comprises the following steps: adding Ganoderma spore powder into 5 times of the wall soaking solution, performing ultrasonic dispersion treatment at 65 deg.C, washing with water, and drying;
step two: then sending the product obtained in the step one into a high-energy electron beam for radiation treatment, and obtaining a material to be subjected to wall breaking after the treatment is finished;
step three: sending the wall-broken material into a ball mill for ball milling treatment, and obtaining ball grinding materials after ball milling is finished;
step four: fully stirring and mixing the ball grinding material and the tremella extract of which the amount is 5 times that of the tremella extract, and then freezing for 3 hours at the temperature of minus 35 ℃;
step five: and then putting the frozen product into an ultrasonic wall breaking machine for wall breaking, obtaining a wall breaking material after wall breaking, and then washing and drying the wall breaking material.
In the first step of this embodiment, the power of the ultrasonic dispersion treatment is 500W, and the ultrasonic time is 20 min.
The preparation method of the coating wall-soaking solution in the embodiment comprises the following steps:
s01: adding 10 parts of chitosan into 25 parts of water, then adding acetic acid, adjusting the pH value to 6.0, then adding 5 parts of sodium alginate, and stirring and fully dispersing to obtain a wall-soaking main agent solution;
s02: the montmorillonite is sent into a thermal reaction furnace for reaction for 30min, the reaction is finished, then ball milling is carried out for 20min at the speed of 1200r/min, and the modified montmorillonite is obtained after the ball milling is finished;
s03: adding the modified montmorillonite into 4 times of the wall-soaking main agent solution, stirring at 65 ℃ for 40min, stirring for 550r/min, and finishing stirring to obtain the protective wall-soaking solution.
In the present embodiment, the rotation speed of the S01 stirring and dispersing fully is 1500r/min, and the stirring time is 30 min.
The temperature of the thermal reaction furnace of this example was 600 ℃.
The high-energy electron beam radiation of this example was Co gamma rays, and the radiation dose was 20 kGy.
The ball milling speed of the ball mill of the embodiment is 700r/min, the ball milling time is 25min, and the ball milling temperature is 10 ℃.
The preparation method of the tremella extract in this embodiment includes:
putting the tremella into water for cleaning, draining, putting into water vapor at 95 ℃ for processing for 20min, putting into 5 times of water for extraction, and obtaining the tremella extract after extraction.
The temperature of the extraction treatment in this example was 100 ℃ and the extraction time was 2 hours.
Example 3.
The invention provides an ultralow-temperature wall breaking method for ganoderma lucidum spore powder, which comprises the following steps:
the method comprises the following steps: adding Ganoderma spore powder into 4 times of the wall soaking solution, performing ultrasonic dispersion treatment at 50 deg.C, washing with water, and drying;
step two: then sending the product obtained in the step one into a high-energy electron beam for radiation treatment, and obtaining a material to be subjected to wall breaking after the treatment is finished;
step three: sending the wall-broken material into a ball mill for ball milling treatment, and obtaining ball grinding materials after ball milling is finished;
step four: fully stirring and mixing the ball grinding material and the tremella extract with the weight of 4 times, and then freezing for 2.5 hours at the temperature of minus 35 ℃;
step five: and then putting the frozen product into an ultrasonic wall breaking machine for wall breaking, obtaining a wall breaking material after wall breaking, and then washing and drying the wall breaking material.
In the first step of this embodiment, the power of the ultrasonic dispersion treatment is 400W, and the ultrasonic time is 15 min.
The preparation method of the coating wall-soaking solution in the embodiment comprises the following steps:
s01: adding 7.5 parts of chitosan into 22.5 parts of water, then adding acetic acid, adjusting the pH value to 5.5, then adding 3.5 parts of sodium alginate, and fully stirring and dispersing to obtain a wall soaking main agent solution;
s02: feeding montmorillonite into a thermal reaction furnace for reaction for 25min, finishing the reaction, then carrying out ball milling for 15min at the speed of 1000r/min, and finishing the ball milling to obtain modified montmorillonite;
s03: adding the modified montmorillonite into 3 times of the wall-soaking main agent solution, stirring at 60 ℃ for 30min, stirring for 500r/min, and stirring to obtain the protective wall-soaking solution.
In the present embodiment, the rotation speed of the S01 for sufficient stirring and dispersion is 1250r/min, and the stirring time is 25 min.
The temperature of the thermal reaction furnace of this example was 550 ℃.
The high-energy electron beam radiation of this example was Co gamma rays with a radiation dose of 15 kGy.
The ball milling speed of the ball mill of the embodiment is 600r/min, the ball milling time is 20min, and the ball milling temperature is 7.5 ℃.
The preparation method of the tremella extract in this embodiment includes:
putting the tremella into water for cleaning, draining, putting into water vapor at 90 ℃ for processing for 15min, putting into 4 times of water for extraction, and obtaining the tremella extract after extraction.
The temperature of the extraction treatment in this example was 100 ℃ and the extraction time was 1.5 hours.
The product of the invention was further tested by comparative example for properties:
comparative example 1.
Unlike example 3, no coating wall-soaking solution was added.
Comparative example 2.
The difference from the example 3 is that the modified montmorillonite is not added into the wall-soaking solution.
Comparative example 3.
Different from the embodiment 3, the preparation method of the protective wall soaking solution is different; 10 parts of chitosan is added into 25 parts of ethanol solvent, then phosphoric acid is added, the pH is adjusted to 5.0, and then 3 parts of silicon dioxide is added, so as to obtain the wall-soaking solution.
Comparative example 4.
Unlike example 3, no high energy electron beam treatment was used.
Comparative example 5.
The difference from example 3 is that no white fungus extract was used.
Detecting the wall breaking rate of the treated ganoderma lucidum spore powder by adopting' determination of NY/T1677-;
the products of examples 1-3 and comparative examples 1-5 were subjected to a performance test;
as can be seen from comparative examples 1 to 5 and examples 1 to 3;
the wall breaking rate of the product and the contents of ganoderan and triterpene are slightly improved without adding the wall protecting wall soaking solution, the wall breaking rate of the product and the contents of ganoderan and triterpene are not greatly influenced by adding the wall protecting wall soaking solution, and the wall breaking rate of the product and the contents of ganoderan and triterpene are slightly influenced by treating the wall protecting wall soaking solution and the tremella extract.
The high-energy electron beam treatment is not adopted, so that the wall breaking rate, the ganoderan content and the triterpene content of the product are obviously reduced, and the wall breaking rate and the ganoderan dissolution effect can be improved by the high-energy electron beam treatment;
the ganoderma lucidum polysaccharide of the products of examples 1-3 and comparative examples 1-5 is adopted to carry out free radical scavenging capacity test, and the content of the ganoderma lucidum polysaccharide is 2.83g/100 g;
as can be seen from comparative examples 1 to 5 and examples 1 to 3;
the method is characterized in that the protective wall-soaking liquid is not added, the DPPH free radical clearance rate and the ABTS free radical clearance rate of the product are obviously reduced, meanwhile, the modified montmorillonite is not added, the preparation methods of the protective wall-soaking liquid are different, the DPPH free radical clearance rate and the ABTS free radical clearance rate are different, and the protective wall-soaking liquid has the effects of obviously improving the DPPH free radical clearance rate and the ABTS free radical clearance rate;
the high-energy electron beam treatment is not adopted, the clearance rate of the free radical clearance of the product is improved, and meanwhile, the tremella extract is adopted for treatment, so that the tremella extract has a synergistic effect, can be matched with the protective wall soaking liquid together, and the clearance rate of the free radical is enhanced;
the comparative examples 1 to 5 show that the high-energy electron beam treatment can destroy the free radical clearance rate, but can improve the wall breaking rate and the ganoderan content of the product, and the protective wall soaking solution and the tremella extract of the invention are adopted for synergistic action, so that the destroyed free radical clearance rate is compensated, and the free radical clearance rate of the product is improved.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. An ultralow-temperature wall breaking method for ganoderma lucidum spore powder is characterized by comprising the following steps:
the method comprises the following steps: adding Ganoderma spore powder into 3-5 times of the wall soaking solution, performing ultrasonic dispersion treatment at 45-65 deg.C, washing with water, and drying;
step two: then sending the product obtained in the step one into a high-energy electron beam for radiation treatment, and obtaining a material to be subjected to wall breaking after the treatment is finished;
step three: sending the wall-broken material into a ball mill for ball milling treatment, and obtaining ball grinding materials after ball milling is finished;
step four: fully stirring and mixing the ball grinding material and the tremella extract with the volume of 3-5 times of the tremella extract, and then freezing for 2-3h at-35 ℃;
step five: and then putting the frozen product into an ultrasonic wall breaking machine for wall breaking, obtaining a wall breaking material after wall breaking, and then washing and drying the wall breaking material.
2. The ultra-low temperature wall breaking method of ganoderma lucidum spore powder as claimed in claim 1, wherein the power of the ultrasonic dispersion treatment in the first step is 300- "500W", and the ultrasonic time is 10-20 min.
3. The ultra-low temperature wall breaking method of ganoderma lucidum spore powder as claimed in claim 1, wherein the preparation method of the coating wall soaking solution comprises the following steps:
s01: adding 5-10 parts of chitosan into 20-25 parts of water, then adding acetic acid, adjusting the pH value to 5.0-6.0, then adding 2-5 parts of sodium alginate, and stirring and fully dispersing to obtain a wall-soaking main agent solution;
s02: the montmorillonite is sent into a thermal reaction furnace for reaction for 20-30min, the reaction is finished, then ball milling is carried out for 10-20min at the speed of 800-;
s03: adding the modified montmorillonite into 2-4 times of the wall-soaking main agent solution, stirring at 55-65 ℃ for 20-40min, stirring at 450-550r/min, and obtaining the protective wall-soaking solution after the stirring is finished.
4. The ultra-low temperature wall-breaking method of ganoderma lucidum spore powder as claimed in claim 3, wherein the rotation speed of the S01 for sufficient stirring and dispersion is 1000-1500r/min, and the stirring time is 20-30 min.
5. The ultra-low temperature wall-breaking method of ganoderma lucidum spore powder as claimed in claim 3, wherein the temperature of the thermal reaction furnace is 500-600 ℃.
6. The ultra-low temperature wall breaking method of ganoderma lucidum spore powder as claimed in claim 1, wherein the high energy electron beam radiation is Co gamma ray, and the radiation dose is 10-20 kGy.
7. The ultra-low temperature wall breaking method of ganoderma lucidum spore powder as claimed in claim 1, wherein the ball milling rotation speed of the ball mill is 500-700r/min, the ball milling time is 15-25min, and the ball milling temperature is 5-10 ℃.
8. The ultra-low temperature wall breaking method for ganoderma lucidum spore powder according to claim 1, wherein the preparation method for the tremella extract comprises the following steps:
cleaning Tremella in water, draining, treating in 85-95 deg.C steam for 10-20min, extracting in 3-5 times of water, and collecting Tremella extractive solution.
9. The ultra-low temperature wall breaking method of ganoderma lucidum spore powder as claimed in claim 8, wherein the temperature of the extraction treatment is 100 ℃, and the extraction time is 1-2 h.
10. The ultra-low temperature wall-breaking method of ganoderma lucidum spore powder as claimed in claim 1, wherein the temperature of the ultrasonic wall-breaking machine is 45-55 ℃ and the processing time is 25-35 min.
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