CN114885769A - Planting method for increasing crop yield under stress of heavy metal pollution - Google Patents
Planting method for increasing crop yield under stress of heavy metal pollution Download PDFInfo
- Publication number
- CN114885769A CN114885769A CN202210399720.4A CN202210399720A CN114885769A CN 114885769 A CN114885769 A CN 114885769A CN 202210399720 A CN202210399720 A CN 202210399720A CN 114885769 A CN114885769 A CN 114885769A
- Authority
- CN
- China
- Prior art keywords
- heavy metal
- crops
- small peptide
- soil
- planting method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 39
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 claims abstract description 66
- 102000014187 peptide receptors Human genes 0.000 claims abstract description 48
- 108010011903 peptide receptors Proteins 0.000 claims abstract description 48
- 239000002689 soil Substances 0.000 claims abstract description 37
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims abstract description 33
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 claims abstract description 32
- 230000014509 gene expression Effects 0.000 claims abstract description 19
- 230000012010 growth Effects 0.000 claims abstract description 12
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 11
- 229930195732 phytohormone Natural products 0.000 claims abstract description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 6
- 241000605008 Spirillum Species 0.000 claims abstract description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 22
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims description 14
- 244000221633 Brassica rapa subsp chinensis Species 0.000 claims description 14
- 238000005507 spraying Methods 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 241000589938 Azospirillum brasilense Species 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 3
- 235000013311 vegetables Nutrition 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 2
- 230000000855 fungicidal effect Effects 0.000 claims 1
- 239000000417 fungicide Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 9
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000012216 screening Methods 0.000 abstract description 3
- 238000011160 research Methods 0.000 abstract description 2
- 241000219195 Arabidopsis thaliana Species 0.000 description 21
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 15
- 241000219194 Arabidopsis Species 0.000 description 11
- 230000002018 overexpression Effects 0.000 description 11
- 238000012217 deletion Methods 0.000 description 10
- 230000037430 deletion Effects 0.000 description 10
- 238000011109 contamination Methods 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 4
- 210000004899 c-terminal region Anatomy 0.000 description 4
- 229910052793 cadmium Inorganic materials 0.000 description 4
- 230000002950 deficient Effects 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 241000219198 Brassica Species 0.000 description 3
- 240000007124 Brassica oleracea Species 0.000 description 3
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 3
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 3
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 3
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 3
- 229930002875 chlorophyll Natural products 0.000 description 3
- 235000019804 chlorophyll Nutrition 0.000 description 3
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000000243 photosynthetic effect Effects 0.000 description 3
- 238000005067 remediation Methods 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 235000003351 Brassica cretica Nutrition 0.000 description 2
- 235000003343 Brassica rupestris Nutrition 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000219315 Spinacia Species 0.000 description 2
- 235000009337 Spinacia oleracea Nutrition 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 235000010460 mustard Nutrition 0.000 description 2
- 229910052759 nickel Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- LMSDCGXQALIMLM-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;iron Chemical compound [Fe].OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O LMSDCGXQALIMLM-UHFFFAOYSA-N 0.000 description 1
- JLIDBLDQVAYHNE-LXGGSRJLSA-N 2-cis-abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\C1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-LXGGSRJLSA-N 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 241000589941 Azospirillum Species 0.000 description 1
- 235000011331 Brassica Nutrition 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229910001018 Cast iron Inorganic materials 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 230000003185 calcium uptake Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010668 complexation reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000008260 defense mechanism Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000004941 influx Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000011133 lead Substances 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- 230000019516 nonphotochemical quenching Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000011378 penetrating method Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 229910000406 trisodium phosphate Inorganic materials 0.000 description 1
- 235000019801 trisodium phosphate Nutrition 0.000 description 1
- 238000004017 vitrification Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Forests & Forestry (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Biotechnology (AREA)
- Ecology (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental & Geological Engineering (AREA)
- Soil Sciences (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The application discloses a planting method for improving crop yield under heavy metal pollution stress, which comprises the following steps: planting crops in a heavy metal polluted farmland, and periodically applying a nitrogen fixing spirillum brasilense microbial inoculum or phytohormone abscisic acid in the growth period of the crops; the crops have low expression of carboxyl terminal coding small peptide receptors. Through a large number of researches, the application discovers that the method can reasonably apply the exogenous ABA by screening the plants with the low expression level of the carboxyl terminal coding small peptide receptor, enhance and improve the crop yield in the heavy metal polluted farmland, and is expected to promote the application effect of the ABA technology in the heavy metal polluted soil.
Description
Technical Field
The invention relates to the technical field of safe production of heavy metal contaminated soil, in particular to a planting method for increasing crop yield under the stress of heavy metal contamination.
Background
Heavy metals are commonly used in many industrial sectors, such as in the fields of batteries, metal plating, pigments, cast iron and metal finishes, where considerable amounts of heavy metals are discharged into the environment, resulting in heavy metal contamination of the soil. The latest edition national soil pollution condition survey bulletin jointly issued by the environmental protection department and the national soil resources department shows that the exceeding rate of the heavy metal point position of the soil in China reaches 13.3 percent. Wherein the point position overproof rates of cadmium, lead, zinc and nickel are respectively 7.0%, 1.5%, 0.9% and 4.8%. Among the heavy metals, the pollution by Cd in soil is particularly prominent, the point position exceeding rate is highest, and the heavy metals relate to 11 areas (plum-fig, etc. cadmium polluted plant restoration technology, biological process 2014,4(4):61-66) in 25 provinces.
Large areas of Cd-contaminated land are inevitably used for agricultural production. Cd has concealment, high biological mobility and biological toxicity, and can be rapidly absorbed and accumulated by crop roots in a large amount in a farmland to influence the growth and development of crops and cause crop yield reduction. For example, 60mg kg -1 The dry weight yield of spinach in Cd-contaminated sandy and silty soils was reduced by 67% and 34%, respectively (Dheri et al. influx of phosphorus application on growth and calcium uptake of spinach in two calcium-associated soils. journal of Plant Nutrition and Soil Science,2007,170(4): 495-499); 50mg kg -1 Reduction of mustard dry weight in Cd-contaminated soil by 37% (Anju et al. Sulphur protects mustard (Brassica ca. campestris L.) from cadmii. toxin by improving leaf ascorate and glutamaterone. plant Growth Regulation,2008,54(3): 271-279); 1. 3 and 5mg kg -1 The fresh weight of the leaf of the pakchoi in the Cd-polluted soil is respectively reduced by 42 percent, 60 percent and 80 percent (Sun Kaixiang, etc. heavy metal ions cadmium (Cd) in the soil have influence on growth and nutritional quality of the pakchoi of crops, geological work boosting ecological civilization construction-2018 academic annual meeting collection of geological society in Zhejiang province, 2018: 21-28). Therefore, the attention is paid to how to relieve the growth stress of Cd in soil on crops, and especially to the improvement of the yield of the crops in Cd-polluted farmlands.
At present, the method and the approach for relieving the growth inhibition of the crops in the Cd-polluted soil at home and abroad are mainly as follows:
(1) physical remediation, such as a soil-penetrating method, an electric remediation method, a vitrification technology and the like. Although the method is rapid and efficient, the method has large project amount and high investment cost, and can change the original soil property to cause the soil fertility to be reduced.
(2) Chemical remediation, namely adding modifiers (such as lime, zeolite and calcium carbonate) into soil, and reducing the mobility and the bioavailability of Cd through the reactions of adsorption, precipitation, complexation and the like of Cd in the soil and chemical reagents, but the Cd still remains in the soil and is easy to reactivate to harm plants, so that secondary pollution is caused.
(3) Bioremediation, which utilizes organisms to cut and purify Cd in soil or reduce the toxicity of Cd. A common method is to plant some super-accumulation plants in a Cd-polluted farmland, and effectively treat the plants after the plants are mature so as to remove or attenuate Cd in soil. Although the method can avoid secondary pollution, the method is often long in period and has higher requirements on natural conditions and artificial conditions.
Therefore, the method for improving the crop yield in situ, which has low cost and high benefit and can realize safe production of Cd-polluted farmland, has important practical significance.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a planting method for improving crop yield under heavy metal pollution stress, wherein exogenous ABA is applied to a heavy metal pollution farmland, and the crop yield is improved by screening the crop with the low expression level of the carboxyl terminal coding small peptide receptor.
In recent years, the growth and development of plants under heavy metal stress are promoted by using abscisic acid (ABA), and the capability of improving the stress resistance of plants against various adversity factors is a potential technical direction. Considering that the increase of the ABA level in the plant body is an important defense mechanism of the plant against Cd stress, the strengthening of the ABA to the relief of the plant growth stress under Cd pollution is probably an effective way to improve the crop yield in the Cd pollution farmland.
According to the invention, compared with wild type Arabidopsis thaliana, the arabidopsis thaliana with the deletion type of the small peptide receptor coded at the carboxyl terminal is 5 mu mol L -1 Applying 0.4 mu mol L under Cd stress condition -1 The biomass increase caused by the exogenous ABA is remarkably promoted, and the ABA also remarkably promotes the alleviation of the photosynthetic stress caused by the Cd. Meanwhile, the growth stress relieving effect of applying exogenous ABA on the carboxyl terminal coding small peptide receptor overexpression type mutant under Cd stress is obviously reduced. Furthermore, the carboxyl-terminal encoding small peptide receptor potentiates the effects of ABA also in Ni contaminated conditions. The results show that the expression levels of different carboxyl terminal coding small peptide receptors are slowed down at exogenous ABA under heavy metal stressThe heavy metal removal has very important effect on the growth stress of plants.
Based on the discovery, the application provides a planting method for improving crop yield under heavy metal pollution stress, which comprises the following steps:
planting crops in a heavy metal polluted farmland or soil, and periodically applying a nitrogen fixing spirillum brasilense or phytohormone abscisic acid in the growth period of the crops; the crops have low expression of carboxyl terminal coding small peptide receptors.
The low expression of the carboxyl terminal coding small peptide receptor means that the expression level of the carboxyl terminal coding small peptide receptor is 10-15 times lower than the normal expression level.
Alternatively, the carboxy-terminal encoded small peptide receptor under-expressed crops can be obtained by directly purchasing under-expressed seeds or by means of genetic engineering routine in the art.
Optionally, the crop is a vegetable.
Optionally, the crop is pakchoi.
Further, the pakchoi is black and big head.
Optionally, the azospirillum brasilense microbial inoculum is prepared from azospirillum brasilense with the preservation number of CGMCC 1.10379.
Optionally, the concentration of viable bacteria in the microbial inoculum is 1-10 multiplied by 10 7 CFU/mL。
Optionally, the microbial inoculum is sprayed on the roots of crops; the first spraying time is when the crops grow to 3-4 true leaves; spraying 3-8 mL of the fertilizer per plant each time; spraying for 1-2 times per week; the total spraying times are 4-8.
Optionally, the heavy metal is Cd or/and Ni.
Optionally, the content of heavy metals in the polluted farmland or soil is 1-5 mg Cd/kg soil or/and 300-700 mg Ni/kg soil.
Further, the heavy metal is Ni; the content of heavy metals in the polluted farmland or soil is 300-700 mg Ni/kg soil. Furthermore, the content of heavy metals in the polluted farmland or soil is 450-550 mg Ni/kg soil.
Optionally, the phytohormone is exfoliatedThe acid is applied in the form of a water culture solution, and the concentration of the phytohormone abscisic acid in the phytohormone abscisic acid water culture solution is 0.2-0.4 mu mol L -1 。
Optionally, transplanting the crops into the hydroponic liquid when the crops grow to 3-4 true leaves, wherein the replacement period of the hydroponic liquid is 1-2 times per week; the total replacement times are 4-8 times.
Compared with the prior art, the invention has at least one of the following beneficial effects:
1) when the method is used, compared with wild arabidopsis, the SPAD value of the arabidopsis with the deletion type of the small peptide receptor coded at the carboxyl terminal after ABA is applied is further increased by 59-67%, and the qN parameter amplification of a stressed plant is improved by 53-55%. The method can strengthen the exogenous ABA to improve the photosynthetic stress of heavy metal pollution on plants.
2) When the method is used, compared with wild arabidopsis, the fresh weights of the overground part and the root system of the arabidopsis with the ABA-matched carboxyl terminal coding small peptide receptor deletion type arabidopsis are further increased by 10-21% and 28-49% respectively. The method can improve the effect of promoting the yield of the plants under the heavy metal stress by using the exogenous ABA.
3) When the method is used, compared with the medium and high expression pakchoi varieties with the small peptide receptor coded at the carboxyl terminal, the yield improvement range of the low expression pakchoi varieties with the small peptide receptor coded at the carboxyl terminal under the Cd or Ni stress by the inoculated ABA producing bacteria is respectively 79-80 percent and 126-143 percent higher. The method can enhance the effect of ABA producing bacteria on promoting the yield of heavy metal stress crops and improve the production economic benefit.
In conclusion, through a large number of researches, the inventor discovers that the method can reasonably apply exogenous ABA by screening plants with low expression level of small peptide receptors coded at the carboxyl terminals, enhance and improve the yield of crops in heavy metal polluted farmlands, and is expected to promote the application effect of the ABA technology in heavy metal polluted soil.
Detailed Description
The technical solutions will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.
Examples 1-3 the plants tested were Colombia-0 wild type and carboxy-terminal encoding small peptide receptor-deleted Arabidopsis thaliana (constructed by T-DNA insertion), specifically, carboxy-terminal encoding small peptide receptor-deleted Arabidopsis thaliana purchased from Arabidopsis Biological Resource Center (ABRC, http:// www.arabidopsis.org) mutant library.
Example 1
The implementation method comprises the following steps:
(1) before the test, a piece of 80-mesh nylon gauze was spread on a sponge with a thickness of about 2cm, soaked with Hoagland's medium, and placed in an incubator (external diameter 175X 115X 65mm), and the Hoagland's medium was added to two thirds of the sponge. The Hoagland culture solution comprises the following components: 2.25mmol L -1 KNO 3 、0.375mmol L -1 K 2 SO 4 、1mmol L -1 CaCl 2 、1mmol L - 1 NaH 2 PO 4 、0.25mmol L -1 MgSO 4 、0.375mmol L -1 (NH 4 ) 2 SO 4 、0.5μmol L -1 ZnSO 4 、25μmol L -1 Fe-EDTA、10μmol L -1 H 3 BO 3 、0.1μmol L -1 CuSO 4 、0.1μmol L -1 (NH 4 ) 6 Mo 7 O 24 And 0.5. mu. mol L -1 MnSO 4 The pH was 5.5. The arabidopsis seeds are placed in a refrigerator at 4 ℃ for vernalization for 48 hours and then spread on the nylon gauze for germination. Plants were all grown in a phytotron under the following conditions: illumination intensity of 50-60 mu mol phosns m -2 s -1 The relative humidity is 70-80%, the photoperiod is 12h/25 ℃/day, and 12h/22 ℃/night.
(2) After Arabidopsis thaliana germinates and grows for 5 weeks, seedlings are transferred to a Cd-containing nutrient solution, and the final concentration of Cd is 5 mu mol L -1 (CdCl 2 Preparation), 0 or 0.4. mu. mol L was added during the culture -1 ABA reagents. In order to maintain the stability of Cd and ABA in the nutrient solution, the solution is replaced every 2-3 days in the embodiment.
(3) Arabidopsis thaliana was harvested after two weeks in greenhouse culture racks. Chlorophyll concentrations were measured with a chlorophyll meter (SPAD-502, japan) and recorded as SPAD readings. Chlorophyll fluorescence measurement (Imag-Max/L, Walz, Germany) is carried out on the overground part of Arabidopsis, the upper, middle and lower parts of each sample leaf are measured once, and the average value is recorded. Three replicates were set up for the experiment. The seedlings were covered with black cloth and dark for 20min before measurement. When in measurement, the leaves are cut off and then are arranged in order, and detection light is started to measure the non-photochemical quenching coefficient qN (light intensity 611mmol m) -2 s -1 ). In addition, the root system and the overground part of the plant are separated, and the fresh weight of the overground part and the fresh weight of the root system are respectively weighed.
Results obtained for Colombia-0 wild-type and carboxy-terminal encoding small peptide receptor-deficient Arabidopsis thaliana are shown in the following table:
wherein, 5 mu mol L -1 SPAD values of wild type and carboxyl terminal encoding small peptide receptor deletion type Arabidopsis thaliana leaves under Cd pollution treatment are shown in Table 1.
TABLE 1
5μmol L -1 Parameters of wild-type and carboxy-terminal encoded small peptide receptor-deleted arabidopsis qN under Cd contamination treatment are shown in table 2.
TABLE 2
5μmol L -1 The fresh weights of wild type and carboxyl terminal encoding small peptide receptor-deficient Arabidopsis thaliana aerial parts under Cd contamination treatment are shown in Table 3.
TABLE 3
5μmol L -1 The fresh root weights of wild type and carboxyl terminal encoding small peptide receptor-deficient arabidopsis thaliana under Cd pollution treatment are shown in Table 4.
TABLE 4
Example 2
The procedure of example 1 was repeated except that "Arabidopsis thaliana lacking a small peptide receptor at the carboxy terminus" in example 1 was changed to "a mutant overexpressing a small peptide receptor at the carboxy terminus" (constructed by the Agrobacterium infiltration method). The results of the obtained Colombia-0 wild-type and carboxyl-terminal-encoded small peptide receptor overexpression mutant Arabidopsis thaliana are shown in the following table:
5μmol L -1 SPAD values of wild type and carboxyl terminal coding small peptide receptor overexpression type mutant Arabidopsis thaliana leaves under Cd pollution treatment are shown in Table 5.
TABLE 5
5μmol L -1 Parameters of wild type and carboxy-terminal encoded small peptide receptor overexpression mutant arabidopsis qN under Cd contamination treatment are shown in table 6.
TABLE 6
5μmol L -1 Cd contamination treatmentThe fresh weights of the lower wild type and the carboxy-terminal encoded small peptide receptor overexpression mutant Arabidopsis thaliana aerial parts are shown in Table 7.
TABLE 7
5μmol L -1 The fresh root weights of wild type and carboxyl terminal encoding small peptide receptor sensitive mutant Arabidopsis thaliana under Cd contamination treatment are shown in Table 8.
TABLE 8
Example 3
"5. mu. mol L" in examples 1 and 2 -1 The final concentration of Cd was "changed to" 12.5. mu. mol L -1 Final Ni concentration ", the rest of the examples 1 and 2. The results of the obtained wild type, carboxyl terminal coding small peptide receptor deletion type and carboxyl terminal coding small peptide receptor overexpression type mutant arabidopsis are shown in the following table:
12.5μmol L -1 SPAD values of the C-terminal encoded small peptide receptor deletion type, the wild type and the C-terminal encoded small peptide receptor overexpression type mutant Arabidopsis thaliana leaves under Ni contamination treatment are shown in Table 9.
TABLE 9
12.5μmol L -1 Parameters of the carboxy-terminal encoded small peptide receptor deletion, wild type and carboxy-terminal encoded small peptide receptor overexpression mutant arabidopsis thaliana qN under Ni contamination treatment are shown in table 10.
Watch 10
12.5μmol L -1 The fresh weights of the carboxy-terminal encoded small peptide receptor-deficient, wild-type and carboxy-terminal encoded small peptide receptor-overexpressing mutant Arabidopsis thaliana aerial parts under Ni-contaminated treatment are shown in Table 11.
TABLE 11
12.5μmol L -1 The fresh root weights of the carboxyl terminal coding small peptide receptor deletion type, the wild type and the carboxyl terminal coding small peptide receptor overexpression type mutant arabidopsis thaliana under the Ni pollution treatment are shown in table 12.
TABLE 12
From the results of examples 1 to 3, it was found that the carboxyl terminal of Arabidopsis thaliana encoding a small peptide receptor deletion type Arabidopsis thaliana was 5. mu. mol L -1 Applying 0.4 mu mol L under Cd stress condition -1 The biomass increase caused by the exogenous ABA is remarkably promoted, and the ABA also remarkably promotes the alleviation of the photosynthetic stress caused by the Cd. Meanwhile, the growth stress relieving effect of applying exogenous ABA on the carboxyl terminal coding small peptide receptor overexpression type mutant under Cd stress is obviously reduced. Furthermore, the carboxyl-terminal encoding small peptide receptor potentiates the effects of ABA also in Ni contaminated conditions. The results show that the expression levels of the small peptide receptors coded by different carboxyl terminals under heavy metal stress play a very important role in relieving the growth stress of heavy metals on plants by exogenous ABA.
Example 4
The plant in the embodiment 1 is changed into a vegetable crop of Chinese cabbage, the variety is black big head, Yangtze river fast and amber, which respectively represents the Chinese cabbage with low, medium and high expression of the carboxyl terminal coding small peptide receptor, and the implementation method is as follows:
the study object of this example was 3mg/kg Cd in soil.
(1) And (5) pot culture experiment. Sterilizing pakchoi seeds with the same size with 10% trisodium phosphate for 20min, and repeatedly washing with distilled water for several times until the seeds are completely washed. Subsequently, the seeds were transferred to seedling pots (outer diameter 330X 260X 50mm) containing Hoagland medium, and when 14d to 3-4 true leaves were cultured, the seeds were transferred to test pots, each pot soil having a mass of 350 g.
(2) And inoculating ABA producing microbial inoculum. The azospirillum brazilian can be purchased from a strain stock such as China general microbiological culture Collection center, and the preservation number is CGMCC 1.10379. The method for culturing and treating the microbial inoculum comprises the following steps: activating and shaking the purchased strains for one week according to a conventional beef extract peptone medium formula (5 g of beef extract, 10g of peptone, 5g of sodium chloride, 5g of glucose, distilled water constant volume to 1L, adjusting pH to 7.0, sterilizing at 121 ℃ for 20min) (the process conditions of activating and shaking the strains are that the inoculated strain bottles are placed in a shaking box to shake the strains at the speed of 150-; and secondly, taking 1000mL of bacterial liquid, centrifuging for 10min at 4000r/min, discarding supernatant, suspending the thalli in phosphate buffer, and centrifuging. The cells are washed twice, and the cell concentration is about 5 multiplied by 10 after being evenly shaken by adding distilled water 7 CFU/mL. 5mL of the microbial inoculum is sprayed on the root of each Chinese cabbage. Administered once per week.
(3) The pakchoi is harvested after being planted in a greenhouse at 25 ℃ for 1-2 months, and the fresh weight is measured.
5μmol L -1 The fresh weight results of the overground parts of the cabbages with low, medium and high expression quantity of the carboxyl terminal coding small peptide receptors under Cd pollution treatment are shown in Table 13, the results in Table 13 show that ABA producing bacteria and the cabbages with low expression quantity of the carboxyl terminal coding small peptide receptors are combined for use, the good synergistic effect is achieved, the cabbages with low expression quantity of the carboxyl terminal coding small peptide receptors are planted in Cd polluted farmlands, and the yield of crops can be obviously improved by applying the ABA producing bacteria.
Watch 13
Example 5
Example 4 was followed except that "3 mg/kg Cd" in example 4 was changed to "500 mg/kg Ni". The results of the obtained wild type, carboxyl terminal coding small peptide receptor deletion type and carboxyl terminal coding small peptide receptor overexpression type mutant Chinese cabbage are shown in the following table:
TABLE 14
As can be seen from the results in tables 13 and 14, the planting method of the present application significantly increases the yield of pakchoi in cadmium-contaminated or nickel-contaminated soils, particularly nickel-contaminated soils.
The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is specific and detailed, but not to be understood as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, and these are all within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A planting method for increasing crop yield under heavy metal pollution stress is characterized by comprising the following steps:
planting crops in a heavy metal polluted farmland, and periodically applying a nitrogen fixing spirillum brazilian fungicide or phytohormone abscisic acid in the growth period of the crops; the crops have low expression of carboxyl terminal coding small peptide receptors.
2. The growing method according to claim 1, wherein the expression level of the carboxy-terminal encoded small peptide receptor in the crop is 10-15 times lower than the normal expression level.
3. The growing method of claim 1, wherein the crop is a vegetable.
4. The growing method according to claim 1, wherein the crop is black head pakchoi.
5. The planting method according to claim 1, wherein the azospirillum brasilense microbial inoculum is a microbial inoculum prepared from azospirillum brasilense with the preservation number of CGMCC 1.10379.
6. The planting method of claim 1, wherein the concentration of viable bacteria in the microbial inoculum is 1-10 x 10 7 CFU/mL; the microbial inoculum is sprayed on the roots of crops; the first spraying time is when the crops grow to 3-4 true leaves; spraying 3-8 mL of the fertilizer per plant each time; spraying for 1-2 times every week; the total spraying times are 4-8.
7. The planting method as claimed in claim 1, wherein the heavy metal is Cd or/and Ni.
8. The planting method of claim 1, wherein the content of heavy metals in the contaminated farmland is 1-5 mg Cd/kg soil or/and 300-700 mg Ni/kg soil.
9. The growing method of claim 1, wherein the heavy metal is Ni; the content of heavy metals in the polluted farmland is 300-700 mg Ni/kg soil.
10. The planting method of claim 1, wherein the phytohormone abscisic acid is applied as a hydroponic solution having a concentration of 0.2-0.4 μmol L of phytohormone abscisic acid -1 (ii) a Transplanting the crops into the hydroponic liquid when the crops grow to 3-4 true leaves, wherein the replacement period of the hydroponic liquid is 1-2 times per week; the total replacement times are 4-8 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210399720.4A CN114885769B (en) | 2022-04-15 | 2022-04-15 | Planting method for improving crop yield under heavy metal pollution stress |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210399720.4A CN114885769B (en) | 2022-04-15 | 2022-04-15 | Planting method for improving crop yield under heavy metal pollution stress |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114885769A true CN114885769A (en) | 2022-08-12 |
| CN114885769B CN114885769B (en) | 2024-02-27 |
Family
ID=82717299
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210399720.4A Active CN114885769B (en) | 2022-04-15 | 2022-04-15 | Planting method for improving crop yield under heavy metal pollution stress |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114885769B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050044594A1 (en) * | 2000-06-16 | 2005-02-24 | Thomas Schmulling | Method for modifying plant morphology, biochemistry and physiology |
| CN1710076A (en) * | 2005-06-01 | 2005-12-21 | 林忠平 | Use of boea crassifolia BcBCP1 gene for breeding drought-salt-tolerant plants |
| US20060230468A1 (en) * | 2005-04-12 | 2006-10-12 | Posco | Novel Pcr family genes which confer tolerance to heavy metals |
| CN105123240A (en) * | 2015-09-16 | 2015-12-09 | 浙江工商大学 | Method for reducing cadmium content of vegetables planted in cadmium contaminated soil |
| CN106397571A (en) * | 2015-07-31 | 2017-02-15 | 李金松 | Novel carboxyl-terminated peptide and long-acting interleukin 7 |
| CN108704928A (en) * | 2018-05-21 | 2018-10-26 | 中南民族大学 | It is a kind of to improve composite drug and its preparation method and application of the plant to Metal uptake turn-over capacity |
| CN109644723A (en) * | 2019-01-25 | 2019-04-19 | 四川大学 | A method of arabidopsis is improved to heavy metal cadmium tolerance |
-
2022
- 2022-04-15 CN CN202210399720.4A patent/CN114885769B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050044594A1 (en) * | 2000-06-16 | 2005-02-24 | Thomas Schmulling | Method for modifying plant morphology, biochemistry and physiology |
| US20060230468A1 (en) * | 2005-04-12 | 2006-10-12 | Posco | Novel Pcr family genes which confer tolerance to heavy metals |
| CN1710076A (en) * | 2005-06-01 | 2005-12-21 | 林忠平 | Use of boea crassifolia BcBCP1 gene for breeding drought-salt-tolerant plants |
| CN106397571A (en) * | 2015-07-31 | 2017-02-15 | 李金松 | Novel carboxyl-terminated peptide and long-acting interleukin 7 |
| CN105123240A (en) * | 2015-09-16 | 2015-12-09 | 浙江工商大学 | Method for reducing cadmium content of vegetables planted in cadmium contaminated soil |
| CN108704928A (en) * | 2018-05-21 | 2018-10-26 | 中南民族大学 | It is a kind of to improve composite drug and its preparation method and application of the plant to Metal uptake turn-over capacity |
| CN109644723A (en) * | 2019-01-25 | 2019-04-19 | 四川大学 | A method of arabidopsis is improved to heavy metal cadmium tolerance |
Non-Patent Citations (1)
| Title |
|---|
| 张然然;张鹏;都韶婷;: "镉毒害下植物氧化胁迫发生及其信号调控机制的研究进展", 应用生态学报, no. 03, 31 March 2016 (2016-03-31) * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114885769B (en) | 2024-02-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Xiaohui et al. | Effects of plant growth-promoting rhizobacteria and N source on plant growth and N and P uptake by tomato grown on calcareous soils | |
| Pishchik et al. | Experimental and mathematical simulation of plant growth promoting rhizobacteria and plant interaction under cadmium stress | |
| Khaitov et al. | Effect of chickpea in association with Rhizobium to crop productivity and soil fertility | |
| Suman et al. | Improving sugarcane growth and nutrient uptake by inoculating Gluconacetobacter diazotrophicus | |
| CN102046778A (en) | Bacterium capable of reducing heavy metal content in plant | |
| CN110624949B (en) | Method for repairing excessive phosphorus pollution of phosphorite waste land by combining indigenous microorganisms and plants | |
| CN112501061A (en) | Microbial agent for wheat planting | |
| CN112094771B (en) | Bacillus cereus B-28 and application thereof | |
| CN107094557A (en) | A kind of method for reducing rice grain heavy metal cadmium content | |
| Salehi et al. | A high internal phosphorus use efficiency in tea (Camellia sinensis L.) plants | |
| CN113373180A (en) | Nano-selenium synthetic active bacterial liquid of bacillus amyloliquefaciens, preparation method and application thereof | |
| Wei et al. | Ericoid mycorrhizal fungi as biostimulants for improving propagation and production of ericaceous plants | |
| CN108124566A (en) | Subtract the method for applying synergy in arrow Kuo peas in cigarette the crop rotation of Rhizobium Inoculation | |
| Fages et al. | Effects of inoculation with Bacillus circulans and Azospirillum lipoferum on crop-yield in field grown maize | |
| CN110252803B (en) | Cadmium-contaminated soil composite passivator and application thereof | |
| CN116121147B (en) | Chenopodium ambrosioides seed endophytic Larimol agrobacterium and application thereof | |
| US11674118B2 (en) | PGPR compositions and methods for improved cultivation of tomato and potato species | |
| CN110157643B (en) | Bacterium H4C8 for reducing cadmium content in wheat and application thereof | |
| CN114437986B (en) | Bacillus seawater and screening method and application thereof | |
| CN102911900A (en) | Ardisia japonica phyllobacterium RC6b and application of same in soil remediation | |
| CN114885769A (en) | Planting method for increasing crop yield under stress of heavy metal pollution | |
| CN115820477A (en) | Plant growth promoting agrobacterium and application | |
| CN114405989A (en) | Method for promoting cadmium absorption of cotton and application thereof | |
| CN113583917A (en) | Compound microbial agent for ecological restoration and preparation method thereof | |
| EP4006173A1 (en) | Microbial compositions based on native rhizobia strains and method of improving legume pastures in acid soils with manganese toxicity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |