CN114885769B - Planting method for improving crop yield under heavy metal pollution stress - Google Patents

Planting method for improving crop yield under heavy metal pollution stress Download PDF

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CN114885769B
CN114885769B CN202210399720.4A CN202210399720A CN114885769B CN 114885769 B CN114885769 B CN 114885769B CN 202210399720 A CN202210399720 A CN 202210399720A CN 114885769 B CN114885769 B CN 114885769B
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peptide receptor
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CN114885769A (en
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都韶婷
李佳欣
田佳赢
张思宇
李贝尔
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Zhejiang Shuren University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/15—Leaf crops, e.g. lettuce or spinach 
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C2101/00—In situ
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Forests & Forestry (AREA)
  • Pest Control & Pesticides (AREA)
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  • Biodiversity & Conservation Biology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Soil Sciences (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The application discloses a planting method for improving crop yield under heavy metal pollution stress, which comprises the following steps: planting crops in heavy metal polluted farmlands, and periodically applying azoospiram brasiliensis inoculant or plant hormone abscisic acid in the growth cycle of the crops; the crop is a crop with low expression of the carboxyl terminal coded small peptide receptor. A great number of researches show that the method can reasonably prepare exogenous ABA by screening plants with low expression level of the carboxyl terminal coding small peptide receptor, strengthen and improve the yield of crops in heavy metal polluted farmlands, and is hopeful to be an application effect of the advanced ABA technology in heavy metal polluted soil.

Description

Planting method for improving crop yield under heavy metal pollution stress
Technical Field
The invention relates to the technical field of safe production of heavy metal contaminated soil, in particular to a planting method for improving crop yield under heavy metal contaminated stress.
Background
Heavy metals are commonly used in many industrial sectors, such as in the fields of batteries, metal plating, pigments, cast iron and metal finishing, and considerable amounts of heavy metals are discharged into the environment, resulting in heavy metal pollution of the soil. The latest edition of national soil pollution Condition investigation publication issued by the combination of the environmental protection department and the national resource department shows that the exceeding rate of the heavy metal point of the soil in China reaches 13.3 percent. Wherein the point location superscalar rates of cadmium, lead, zinc and nickel are 7.0%, 1.5%, 0.9% and 4.8% respectively. Among the heavy metals, the Cd pollution of the soil is particularly remarkable, the point position exceeding rate is highest, and the method relates to 25 areas (Li Yanji and the like, cadmium pollution phytoremediation technology, biological process, 2014,4 (4): 61-66) in 11 provinces.
Large areas of Cd contaminated land are inevitably used in agricultural production. Cd has concealment, higher biological mobility and biological toxicity, can be rapidly absorbed and accumulated in a large amount by crop root systems in farmlands, and influences the growth and development of crops, so that the yield of the crops is reduced. For example, 60mg kg -1 The dry weight yield of spinach in Cd-contaminated sandy and silty soil was reduced by 67% and 34%, respectively (Dheri et al Influence of phosphorus application on growth and cadmium uptake of spinach in two cadmium-contained soil. Journal of Plant Nutr)ition and Soil Science,2007,170(4):495-499)；50mg kg -1 The dry weight of mustard in Cd contaminated soil was reduced by 37% (Anjum et al Sulphur protects mustard (Brassica campestris L.)) from cadmium toxicity by improving leaf ascorbate and glutethione, plant Growth Regulation,2008,54 (3): 271-279); 1. 3 and 5mg kg -1 The fresh weight of the leaf of the green Chinese cabbage in the Cd polluted soil is reduced by 42%, 60% and 80% (Sun Kaixiang, etc.. Heavy metal ion cadmium (Cd) in the soil has an influence on the growth and the nutrition quality of the crop green Chinese cabbage, geological work boosting ecological civilization construction, the academic society of the geological society of Zhejiang province, 2018:21-28). Therefore, how to relieve the growth stress of Cd in soil on crops, especially to improve the yield of Cd-polluted farmland crops is of great concern.
At present, the method and the path for relieving the inhibition of the growth of crops in Cd polluted soil at home and abroad mainly comprise the following steps:
(1) Physical repair methods such as earth-moving method, electric repair method, vitrification technique, etc. The method is rapid and efficient, but has large engineering quantity and high investment cost, and can change the original soil property to cause the soil fertility to be reduced.
(2) Chemical restoration, adding modifier (such as lime, zeolite and calcium carbonate) into soil, and reducing mobility and bioavailability of Cd by adsorption, precipitation, complexation and other reactions of Cd and chemical reagent in the soil, wherein Cd still remains in the soil, so that the plant is easily activated again to cause secondary pollution.
(3) Bioremediation, utilizing organisms to cut, purify Cd in soil or reduce Cd toxicity. A relatively common method is to plant some super-accumulated plants in a Cd-polluted farmland, and treat them effectively after they are mature, so as to remove or attenuate Cd in the soil. Although the method can avoid secondary pollution, the method has long period and high requirements on natural conditions and artificial conditions.
Therefore, the method for in-situ crop yield improvement, which has low cost and high benefit and can realize safe production of Cd-polluted farmland, has important practical significance.
Disclosure of Invention
The invention solves the technical problem of providing a planting method for improving crop yield under heavy metal pollution stress, which applies exogenous ABA in heavy metal polluted farmland, combines screening of crops with low expression level of carboxyl terminal coded small peptide receptor, and improves crop yield.
In recent years, the use of plant hormone abscisic acid (ABA) to promote plant growth and development under heavy metal stress and to improve the capability of plants to resist various adversity factor stresses has become a potential technical direction. Considering that the increase of the ABA level in the plant body is an important defense mechanism of the plant for coping with Cd stress, strengthening the alleviation of the plant growth stress under Cd pollution by the ABA is likely to be an effective way for improving the crop yield in Cd polluted farmlands.
The invention discovers that compared with wild arabidopsis thaliana, the carboxy terminal coding small peptide receptor deletion type arabidopsis thaliana has the amino acid sequence of 5 mu mol L -1 Under Cd stress conditions, 0.4. Mu. Mol L was applied -1 The biomass increase caused by exogenous ABA is greatly promoted, and the alleviation of photosynthetic stress caused by Cd by ABA is also greatly promoted. Meanwhile, the growth stress relieving effect of the carboxyl terminal coding small peptide receptor over-expression type mutant under Cd stress by applying exogenous ABA is obviously reduced. Furthermore, the effect of the carboxy-terminal encoded small peptide receptor to strengthen ABA also exists under Ni-contaminated conditions. The results show that different expression levels of the carboxyl terminal coded small peptide receptor under heavy metal stress play a very important role in relieving growth stress of heavy metal on plants by exogenous ABA.
Based on this finding, the present application provides a planting method for improving crop yield under heavy metal pollution stress, comprising:
planting crops in heavy metal polluted farmlands or soil, and periodically applying azoospiram brasiliensis inoculant or plant hormone abscisic acid in the growth cycle of the crops; the crop is a crop with low expression of the carboxyl terminal coded small peptide receptor.
The low expression of the carboxyl terminal coded small peptide receptor means that the expression quantity of the carboxyl terminal coded small peptide receptor is 10-15 times lower than the normal expression quantity.
Alternatively, the carboxy-terminal encoded small peptide receptor low expression crop can be obtained by direct purchase of low expression seed or by means of genetic engineering conventional in the art.
Optionally, the crop is a vegetable.
Optionally, the crop is chinese cabbage.
Further, the cabbage is black head.
Optionally, the azoospiram brasiliensis microbial inoculum is prepared from azoospiram brasiliensis with a collection number of CGMCC 1.10379.
Optionally, the concentration of the viable bacteria in the microbial inoculum is 1-10 multiplied by 10 7 CFU/mL。
Optionally, the microbial inoculum is sprayed on the roots of crops; the first spraying time is when the crop grows to 3-4 true leaves; 3-8 mL/plant is sprayed each time; spraying for 1-2 times per week; the total spraying times are 4-8 times.
Optionally, the heavy metal is Cd or/and Ni.
Optionally, the content of heavy metals in the polluted farmland or soil is 1-5 mg Cd/kg soil or/and 300-700 mg Ni/kg soil.
Further, the heavy metal is Ni; the heavy metal content in the polluted farmland or soil is 300-700 mg Ni/kg soil. Further, the heavy metal content in the polluted farmland or soil is 450-550 mg Ni/kg soil.
Optionally, the plant hormone abscisic acid is applied in the form of a water culture solution, and the concentration of the plant hormone abscisic acid in the plant hormone abscisic acid culture solution is 0.2-0.4 mu mol L -1 。
Optionally, transplanting the crops into the water culture solution when the crops grow to 3-4 true leaves, wherein the water culture solution replacement period is 1-2 times/week; the total number of replacement times is 4-8.
Compared with the prior art, the invention has at least one of the following beneficial effects:
1) When the method is used, compared with wild type arabidopsis, the SPAD value of the carboxy terminal coding small peptide receptor deletion type arabidopsis after ABA is applied is further increased by 59-67%, and the qN parameter of the stress plant is increased by 53-55%. This shows that the method can strengthen exogenous ABA to improve photosynthetic stress of heavy metal pollution to plants.
2) When the method is used, compared with wild type arabidopsis thaliana, the fresh weight of the aerial parts and root systems of the arabidopsis thaliana with the deletion of the small peptide receptor encoded at the carboxyl terminal after ABA is applied is further increased by 10-21% and 28-49% respectively. This demonstrates that the effect of exogenous ABA on promoting plant yield under heavy metal stress can be improved by the method.
3) When the method is used, compared with the variety of the high-expression cabbage in the carboxyl terminal coding small peptide receptor, the yield improvement amplitude of the variety of the low-expression cabbage inoculated with the ABA-producing bacteria under Cd or Ni stress is respectively higher than 79-80% and 126-143%. The method can strengthen the effect of ABA-producing bacteria in promoting the yield of heavy metal stress crops and improve the economic benefit of production.
In conclusion, the inventor finds that the method can reasonably match exogenous ABA by screening plants with low expression level of the carboxyl terminal coded small peptide receptor through a large number of researches, so that the crop yield in heavy metal polluted farmlands is enhanced and improved, and the application effect of the ABA technology in heavy metal polluted soil is expected to be promoted.
Detailed Description
The following description of the embodiments will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all, of the embodiments of the present application. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the present disclosure, are within the scope of the present disclosure.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.
The test plants in examples 1 to 3 were Colombia-0 wild-type and C-terminal encoded small peptide receptor deleted Arabidopsis thaliana (T-DNA insertion construction), specifically C-terminal encoded small peptide receptor deleted Arabidopsis thaliana was purchased from the Arabidopsis Biological Resource Center (ABRC, http:// www.arabidopsis.org) mutant pool.
Example 1
The implementation method comprises the following steps:
(1) A piece of 80-mesh nylon gauze was laid on a sponge having a thickness of about 2cm before the start of the test, and was immersed in a Hoagland culture solution, and then placed in an incubator (outer diameter: 175X 115X 65 mm), and Hoagland culture solution was added to two thirds of the sponge. The Hoagland culture solution comprises the following components: 2.25mmol L -1 KNO 3 、0.375mmol L -1 K 2 SO 4 、1mmol L -1 CaCl 2 、1mmol L - 1 NaH 2 PO 4 、0.25mmol L -1 MgSO 4 、0.375mmol L -1 (NH 4 ) 2 SO 4 、0.5μmol L -1 ZnSO 4 、25μmol L -1 Fe-EDTA、10μmol L -1 H 3 BO 3 、0.1μmol L -1 CuSO 4 、0.1μmol L -1 (NH 4 ) 6 Mo 7 O 24 And 0.5. Mu. Mol L -1 MnSO 4 The pH was 5.5. The Arabidopsis seeds are placed in a refrigerator at 4 ℃ for vernalization for 48 hours and then spread on the nylon gauze for germination. Plants were all cultivated in a climatic chamber under the following conditions: the illumination intensity is 50-60 mu mol photons m -2 s -1 The relative humidity is 70-80%, the photoperiod is 12h/25 ℃/day, and the photoperiod is 12h/22 ℃/night.
(2) After Arabidopsis thaliana germinated and grown for 5 weeks, the seedlings were transferred to a Cd-containing nutrient solution with a final concentration of Cd of 5. Mu. Mol L -1 (CdCl 2 Formulated), 0 or 0.4. Mu. Mol L was added during the culture -1 ABA reagent. To maintain the stability of Cd and ABA in the nutrient solution, the solution was changed every 2-3d in this example.
(3) Arabidopsis thaliana was harvested after two weeks of planting in a greenhouse culture rack. Chlorophyll concentrations were measured with a chlorophyll meter (SPAD-502, japan) and recorded as SPAD readings. Chlorophyll fluorescence measurement (Imag-Max/L, walz, germany) was performed on the aerial parts of arabidopsis thaliana, and the upper, middle and lower three parts of each sample leaf were measured once, and the average value was recorded. Three replicates were set for the test. The seedlings were dark-treated with black cloth for 20min before measurement. Leaf is used for measuringAfter cutting, the sheets are arranged in order and the detection light is started to measure the non-photochemical quenching coefficient qN (light intensity 611mmol m) -2 s -1 ). In addition, the plant root system and the overground part are separated, and the overground part and the fresh weight of the root system are respectively weighed.
Colombia-0 wild-type and carboxy-terminal encoded small peptide receptor deleted Arabidopsis thaliana gave the following table:
wherein 5. Mu. Mol L -1 The SPAD values of wild-type and carboxy-terminal encoded small peptide receptor deleted arabidopsis leaves under Cd contamination treatment are shown in table 1.
TABLE 1
5μmol L -1 The parameters of wild type and carboxy terminal encoded small peptide receptor deleted Arabidopsis qN under Cd contamination treatment are shown in Table 2.
TABLE 2
5μmol L -1 The fresh weights of the wild type and carboxy terminal encoded small peptide receptor deleted parts of Arabidopsis thaliana under Cd contamination treatment are shown in Table 3.
TABLE 3 Table 3
5μmol L -1 The fresh weights of wild-type and carboxy-terminal encoded small peptide receptor deleted Arabidopsis roots under Cd contamination treatment are shown in Table 4.
TABLE 4 Table 4
Example 2
The procedure of example 1 was repeated except that the "carboxy-terminal encoded small peptide receptor-deleted Arabidopsis thaliana" of example 1 was changed to "carboxy-terminal encoded small peptide receptor-overexpressed mutant" (constructed by Agrobacterium infiltration). The results of the resulting Colombia-0 wild-type and carboxy-terminal encoded small peptide receptor over-expressed mutant Arabidopsis are shown in the following Table:
5μmol L -1 the SPAD values of wild-type and carboxy-terminal encoded small peptide receptor over-expressed mutant arabidopsis leaves under Cd contamination treatment are shown in table 5.
TABLE 5
5μmol L -1 The parameters of wild-type and carboxy-terminal encoded small peptide receptor over-expressed mutant Arabidopsis qN under Cd contamination are shown in Table 6.
TABLE 6
5μmol L -1 The fresh weights of the wild-type and carboxy-terminal encoded small peptide receptor over-expressed mutant Arabidopsis thaliana aerial parts under Cd contamination treatment are shown in Table 7.
TABLE 7
5μmol L -1 The fresh root weights of wild-type and carboxy-terminal encoded small peptide receptor-sensitive mutant Arabidopsis thaliana under Cd contamination treatment are shown in Table 8.
TABLE 8
Example 3
"5. Mu. Mol L" in examples 1 and 2 -1 The final concentration of Cd is "changed to" 12.5. Mu. Mol L -1 Ni final concentration ", the remainder was the same as in examples 1 and 2. The results of the obtained wild-type, carboxy-terminal encoded small peptide receptor deleted mutant and carboxy-terminal encoded small peptide receptor overexpressed mutant arabidopsis are shown in the following table:
12.5μmol L -1 the carboxy-terminal encoded small peptide receptor deleted, wild-type and carboxy-terminal encoded small peptide receptor over-expressed mutant Arabidopsis leaf SPAD values under Ni contamination treatment are shown in Table 9.
TABLE 9
12.5μmol L -1 The parameters of carboxy-terminal encoded small peptide receptor deleted, wild-type and carboxy-terminal encoded small peptide receptor over-expressed mutant Arabidopsis qN under Ni-contamination treatment are shown in Table 10.
Table 10
12.5μmol L -1 The fresh weights of the aerial parts of the carboxy-terminal encoded small peptide receptor deleted mutants, wild-type and carboxy-terminal encoded small peptide receptor overexpressed mutants of Arabidopsis under Ni contamination treatment are shown in Table 11.
TABLE 11
12.5μmol L -1 Carboxy-terminal encoded small peptide receptor deletion, wild-type and carboxy-terminal under Ni contamination treatmentThe fresh root weights of the encoded small peptide receptor over-expressed mutant Arabidopsis thaliana are shown in Table 12.
Table 12
From the results of examples 1 to 3, it was found that the carboxy-terminal encoded small peptide receptor-deleted Arabidopsis thaliana was present at 5. Mu. Mol L as compared with the wild-type Arabidopsis thaliana -1 Under Cd stress conditions, 0.4. Mu. Mol L was applied -1 The biomass increase caused by exogenous ABA is greatly promoted, and the alleviation of photosynthetic stress caused by Cd by ABA is also greatly promoted. Meanwhile, the growth stress relieving effect of the carboxyl terminal coding small peptide receptor over-expression type mutant under Cd stress by applying exogenous ABA is obviously reduced. Furthermore, the effect of the carboxy-terminal encoded small peptide receptor to strengthen ABA also exists under Ni-contaminated conditions. The results show that different expression levels of the carboxyl terminal coded small peptide receptor under heavy metal stress play a very important role in relieving growth stress of heavy metal on plants by exogenous ABA.
Example 4
The method for changing the plant in the embodiment 1 into the vegetable crop Chinese cabbage, wherein the varieties are black head, yangtze river fast and amber, and the Chinese cabbage respectively represents the Chinese cabbage with low, medium and high expression quantity of the carboxyl terminal coded small peptide receptor, and the implementation method comprises the following steps:
the study object of this example was 3mg/kg Cd soil.
(1) Potting experiments. Sterilizing the same size of Chinese cabbage seeds with 10% trisodium phosphate for 20min, and repeatedly cleaning with distilled water for several times until cleaning. Subsequently, the seeds were transferred to a seedling pot (outer diameter 330X 260X 50 mm) containing Hoagland culture solution, and when the cultivation was carried out for 14d to 3-4 true leaves, the seeds were transferred to a test pot, and the mass of each pot soil was 350g.
(2) And (5) inoculating an ABA-producing microbial inoculum. The azoospermia barcina can be purchased from a strain library such as China general microbiological culture collection center, and the like, and the collection number is CGMCC 1.10379. The culturing and treating method of the microbial inoculum comprises the following steps: (1) the purchased strains are processed into beef extract egg according to the conventional methodBai the culture medium formula (beef extract 5g, peptone 10g, sodium chloride 5g, glucose 5g, distilled water to volume of 1L, pH7.0, 121 deg.C sterilization for 20 min) is used for activating and shaking for one week (the process condition of activating and shaking is that the inoculated strain bottle is placed in a shaking box for shaking at 150-200 rpm and 30 deg.C); (2) taking 1000mL of bacterial liquid, centrifuging for 10min at 4000r/min, discarding supernatant, suspending bacterial cells in phosphate buffer solution, and centrifuging. Washing twice, adding distilled water, shaking, and concentrating to about 5×10 7 CFU/mL. Spraying 5mL of the microbial inoculum on the root of each plant of the cabbage. Is administered once a week.
(3) The fresh weight of the cabbages is measured after the cabbages are planted in a greenhouse at 25 ℃ for 1-2 months and harvested.
5μmol L -1 The fresh weight results of the overground parts of the cabbages with low, medium and high expression levels of the carboxyl end coded small peptide receptors under the Cd pollution treatment are shown in the table 13, and the results in the table 13 show that the ABA-producing bacteria and the cabbages with low expression levels of the carboxyl end coded small peptide receptors are combined for use, so that the method has good synergistic effect, the cabbages with low expression levels of the carboxyl end coded small peptide receptors are planted in Cd polluted farmland, and the ABA-producing bacteria are applied at the same time, so that the yield of crops can be remarkably improved.
TABLE 13
Example 5
The procedure of example 4 was repeated except that "3mg/kg Cd" in example 4 was changed to "500mg/kg Ni". The results of the obtained wild type, carboxyl terminal encoded small peptide receptor deletion type and carboxyl terminal encoded small peptide receptor over-expression type mutant small cabbage are shown in the following table:
TABLE 14
From the results of tables 13 and 14, the planting method of the present application significantly improved the yield of cadmium-contaminated or nickel-contaminated soil, especially chinese cabbage in nickel-contaminated soil.
The above examples merely represent a few embodiments of the present application, which are described in more detail and are not to be construed as limiting the scope of the invention. It should be noted that it would be apparent to those skilled in the art that various modifications and improvements could be made without departing from the spirit of the present application, which would be within the scope of the present application. Accordingly, the scope of protection of the present application is to be determined by the claims appended hereto.

Claims (7)

1. A planting method for increasing crop yield under heavy metal pollution stress, comprising:
planting crops in heavy metal polluted farmlands, and periodically applying Brazilian nitrogen fixation spiral bacterial agent or plant hormone abscisic acid in the growth cycle of the crops, wherein the Bazilian nitrogen fixation spiral bacterial agent is bacterial agent prepared from Bazilian nitrogen fixation spiral bacterial with the preservation number of CGMCC 1.10379; the crop is a crop with low expression of a carboxyl terminal coded small peptide receptor; the expression quantity of the small peptide receptor coded by the carboxyl terminal in the crop is 10-15 times lower than the normal expression quantity; compared with the variety of crops with medium and high expression of the carboxyl terminal coded small peptide receptor, the yield of crops with low expression of the carboxyl terminal coded small peptide receptor is obviously improved.
2. The planting method of claim 1, wherein the crop is a vegetable.
3. The method of claim 1, wherein the crop is black-headed cabbage.
4. The planting method according to claim 1, wherein the concentration of viable bacteria in the microbial inoculum is 1-10×10 7 CFU/mL; the microbial inoculum is sprayed on the root of crops; the first spraying time is when the crop grows to 3-4 true leaves; spraying 3-8 mL/plant each time; spraying for 1-2 times per week; the total spraying times are 4-8 times.
5. The planting method according to claim 1, wherein the heavy metal is Cd or/and Ni.
6. The planting method according to claim 1, wherein the content of heavy metals in the polluted farmland is 1-5 mg Cd/kg soil or/and 300-700 mg Ni/kg soil.
7. The planting method according to claim 1, wherein the plant hormone abscisic acid is applied in the form of a water culture solution, and the concentration of the plant hormone abscisic acid in the water culture solution is 0.2-0.4 mu mol L -1 The method comprises the steps of carrying out a first treatment on the surface of the Transplanting the crops into the water culture liquid when the crops grow to 3-4 true leaves, wherein the water culture liquid replacement period is 1-2 times per week; the total replacement times are 4-8 times.
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