CN114868599A - Method for accelerating induction of formation of pleurotus ostreatus primordium by using maltose - Google Patents
Method for accelerating induction of formation of pleurotus ostreatus primordium by using maltose Download PDFInfo
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- 240000001462 Pleurotus ostreatus Species 0.000 title claims abstract description 36
- 235000001603 Pleurotus ostreatus Nutrition 0.000 title claims abstract description 36
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 title claims abstract description 34
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 title claims abstract description 34
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 22
- 230000006698 induction Effects 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 15
- 235000007685 Pleurotus columbinus Nutrition 0.000 title claims description 11
- 239000001963 growth medium Substances 0.000 claims abstract description 30
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 235000012015 potatoes Nutrition 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 230000003213 activating effect Effects 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000004080 punching Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 241000233866 Fungi Species 0.000 abstract description 2
- 230000001939 inductive effect Effects 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 description 1
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 239000007640 basal medium Substances 0.000 description 1
- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000003228 hemolysin Substances 0.000 description 1
- 230000028644 hyphal growth Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 229940016667 resveratrol Drugs 0.000 description 1
- 235000021283 resveratrol Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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Abstract
The invention discloses a method for accelerating induction of formation of an oyster mushroom primordium by using maltose, and belongs to the technical field of edible fungi. The invention discloses a method for accelerating induction of oyster mushroom primordium formation by using maltose, which is characterized in that 1.0-2.5 g/L of maltose is added into a PDA culture medium. The method for accelerating induction of the formation of the primordium of the oyster mushroom by using maltose, disclosed by the invention, can accelerate induction of the primordium in an oyster mushroom plate culture medium and improve the proportion of the primordium of an oyster mushroom plate.
Description
Technical Field
The invention relates to the technical field of edible fungi, in particular to a method for accelerating induction of formation of an oyster mushroom primordium by utilizing maltose.
Background
The primordium formation of oyster mushroom (also called pleurotus ostreatus) is an important mark for the transition from vegetative growth (hyphal growth) to reproductive growth (fruiting body growth and development), and is also a key step for finally forming yield and economic benefit. The external conditions for forming the primordium mainly comprise nutritional conditions and environmental conditions, wherein the nutritional conditions mainly comprise carbon, a nitrogen source, a carbon-nitrogen ratio, inorganic salt, growth hormone and the like, and the environmental conditions mainly comprise temperature, illumination, humidity, pH value, oxygen, carbon dioxide and the like. It has been found that many chemicals in Pleurotus ostreatus, such as resveratrol, beta-adenylate, cellular haemolysin, auxin, etc., can induce primordial formation. The carbon source is an important energy source for growth and development of the oyster mushrooms and is a raw material for synthesizing carbohydrates and amino acids. In many studies, carbohydrate metabolic pathways are abnormally active during primordial formation, maltose being one of the most common disaccharides and reducing sugars, and being very soluble in water. Maltose has not been reported to promote the formation of Pleurotus ostreatus primordium.
Therefore, it is an urgent problem to be solved by those skilled in the art to provide a method for accelerating induction of primordium formation of Pleurotus ostreatus using maltose.
Disclosure of Invention
In view of the above, the present invention provides a method for accelerating the induction of the formation of primordia of Pleurotus Ostreatus by using maltose.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for accelerating and inducing formation of oyster mushroom primordium by utilizing maltose is characterized in that 1.0-2.5 g/L of maltose is added into a PDA culture medium.
Further, the method for accelerating and inducing formation of the primordium of the oyster mushroom by using maltose comprises the following steps:
(1) activated culture of strain
Taking out the oyster mushroom strain P99 preserved at low temperature of 4 ℃ or preserved by liquid nitrogen, inoculating the oyster mushroom strain P99 on a flat plate containing a PDA culture medium, and performing activated culture at 25 ℃ for 5-7 days; activating for 1 time;
(2) preparation of culture Medium
Peeling 200g of potatoes, cutting the potatoes into small blocks of 0.5-1 cm, putting the small blocks into a pot filled with 800ml of boiled deionized water, and boiling for 10-15 min; then filtering with 4 layers of gauze, adding 20g of glucose and 15g of agar powder into the filtrate, and heating to completely dissolve the glucose and the agar powder; adding deionized water to a constant volume of 1000mL, and stirring and uniformly mixing to obtain a PDA culture medium; then adding 100ml of PDA culture medium into a triangular flask with the volume of 250ml, adding 1.0-2.5 g/L of maltose into the triangular flask, and sealing a breathable film to obtain the PDA culture medium containing maltose; sterilizing at 121 deg.C for 30 min; placing the sterilized triangular flask in a super clean bench, pouring 20ml of sterilized PDA culture medium containing maltose into a sterile flat plate, and cooling for later use;
(3) hypha culture
Punching a strain block on the edge of the hypha flat plate activated in the step (1) by using a puncher with the diameter of 5mm, then inoculating the strain block into the center of the flat plate culture medium cooled in the step (2), and placing the flat plate culture medium in an incubator at 25 ℃ in the dark for culturing for 5-7 days until the hypha has the diameter of 4-5 cm;
(4) primordial induction
And (4) moving the flat plate cultured in the step (3) until the hypha diameter is 4-5 cm to an incubator at 15 ℃ to induce the primordium, illuminating for 10 hours and darkness for 14 hours, and observing the formation of the primordium.
Further, the oyster mushroom is oyster mushroom strain P99.
According to the technical scheme, compared with the prior art, the method for accelerating induction of primordium formation of oyster mushrooms by utilizing maltose can accelerate induction of primordium in oyster mushroom plate culture medium (for example, the primordium can appear in 8-day oyster mushroom culture medium by adding 1.5g/L maltose into the basal medium after M2 treatment), and can improve the proportion of primordium appearing on oyster mushroom plates (for example, the proportion of the primordium appearing in M1-M4 treatment is between 20-60% after 18 days of induction, and the proportion of the primordium appearing in the contrast is 0 without adding maltose).
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a graph showing 18 days of primordia induction by the addition of maltose (M2) at a concentration of 1.5g/L in accordance with the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for accelerating and inducing formation of oyster mushroom primordium by using maltose comprises the following steps:
(1) activated culture of strain
Taking out the oyster mushroom strain P99 preserved at low temperature of 4 ℃ or preserved by liquid nitrogen, inoculating the oyster mushroom strain P99 on a flat plate containing a PDA culture medium, and performing activated culture at 25 ℃ for 5-7 days; activating for 1 time;
(2) preparation of culture Medium
Peeling 200g of potatoes, cutting the potatoes into small blocks of 0.5-1 cm, putting the small blocks into a pot filled with 800ml of boiled deionized water, and boiling for 10-15 min; then filtering with 4 layers of gauze, adding 20g of glucose and 15g of agar powder into the filtrate, and heating to completely dissolve the glucose and the agar powder; adding deionized water to a constant volume of 1000mL, and stirring and uniformly mixing to obtain a PDA culture medium; then adding 100ml of PDA culture medium into a triangular flask with the volume of 250ml, respectively adding maltose with the concentration of 0, 1.0, 1.5, 2.0 and 2.5g/L into different triangular flasks, and sealing the breathable films to obtain PDA culture medium containing maltose with different concentrations; sterilizing at 121 deg.C for 30 min; placing the sterilized triangular flask in a super clean bench, pouring 20ml of sterilized PDA culture medium containing maltose with different concentrations into a sterile flat plate, and cooling for later use;
(3) hypha culture
Punching a strain block on the edge of the hypha flat plate activated in the step (1) by using a puncher with the diameter of 5mm, then inoculating the strain block into the center of the flat plate culture medium cooled in the step (2), and placing the flat plate culture medium in an incubator at 25 ℃ in the dark for culturing for 5-7 days until the hypha has the diameter of 4-5 cm;
(4) primordial induction
And (4) moving the flat plate cultured to the hypha diameter of 4-5 cm in the step (3) to an incubator at 15 ℃ to induce the primordium, illuminating for 10 hours and darkness for 14 hours, and observing the formation of the primordium, wherein the results are shown in Table 1.
TABLE 1 proportion of primordia appearing on the oyster Mushroom plates under different treatment conditions
| Treatment of | Inducing for 8 days | Inducing for 13 days | Inducing for 18 days | Inducing for 25 days |
| CK(0g/L) | 0 | 0 | 0 | 10％ |
| M1(1.0g/L) | 0 | 0 | 20％ | 40％ |
| M2(1.5g/L) | 40％ | 50％ | 60％ | 80％ |
| M3(2.0g/L) | 0 | 20％ | 40％ | 80％ |
| M4(2.5g/L) | 0 | 0 | 40％ | 60％ |
The results in Table 1 show that 40% of the plates were induced to develop primordia after 8 days with maltose (M2); 60% of the plates developed primordia after 18 days of induction (FIG. 1). After 18 days of induction, all plates added with maltose showed an ortho ratio of 20-60%, and after 25 days of induction, only 10% of the plates showed ortho in the Control (CK).
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (3)
1. A method for accelerating induction of formation of an oyster mushroom primordium by using maltose is characterized in that 1.0-2.5 g/L of maltose is added into a PDA culture medium.
2. The method for accelerating the induction of the formation of primordia of Pleurotus ostreatus using maltose as claimed in claim 1 comprising the steps of:
(1) activated culture of strain
Taking out the oyster mushroom strain P99 preserved at low temperature of 4 ℃ or preserved by liquid nitrogen, inoculating the oyster mushroom strain P99 on a flat plate containing a PDA culture medium, and performing activated culture at 25 ℃ for 5-7 days; activating for 1 time;
(2) preparation of culture Medium
Peeling 200g of potatoes, cutting the potatoes into small blocks of 0.5-1 cm, putting the small blocks into a pot filled with 800ml of boiled deionized water, and boiling for 10-15 min; then filtering with 4 layers of gauze, adding 20g of glucose and 15g of agar powder into the filtrate, and heating to completely dissolve the glucose and the agar powder; adding deionized water to a constant volume of 1000mL, and stirring and uniformly mixing to obtain a PDA culture medium; then adding 100ml of the PDA culture medium into a triangular flask with the volume of 250ml, adding 1.0-2.5 g/L of maltose into the triangular flask, and sealing a breathable film to obtain the PDA culture medium containing maltose; sterilizing at 121 deg.C for 30 min; placing the sterilized triangular flask in a super clean bench, pouring 20ml of sterilized PDA culture medium containing maltose into a sterile flat plate, and cooling for later use;
(3) hypha culture
Punching a strain block on the edge of the hypha flat plate activated in the step (1) by using a puncher with the diameter of 5mm, then inoculating the strain block into the center of the flat plate culture medium cooled in the step (2), and placing the flat plate culture medium in an incubator at 25 ℃ in the dark for culturing for 5-7 days until the hypha has the diameter of 4-5 cm;
(4) primordial induction
And (4) moving the flat plate cultured in the step (3) until the hypha diameter is 4-5 cm to an incubator at 15 ℃ to induce the primordium, illuminating for 10 hours and darkness for 14 hours, and observing the formation of the primordium.
3. The method for accelerating induction of primordium formation of Pleurotus ostreatus using maltose according to claim 1 or 2, wherein the Pleurotus ostreatus is Pleurotus ostreatus strain P99.
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|---|---|---|---|---|
| CN101659578A (en) * | 2009-09-18 | 2010-03-03 | 鲁东大学 | Artificial culture method of Kyushu cordyceps sinensis and culture medium thereof |
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- 2022-06-09 CN CN202210653315.0A patent/CN114868599A/en active Pending
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