CN114867852A - V型crispr-cas碱基编辑器及其使用方法 - Google Patents

V型crispr-cas碱基编辑器及其使用方法 Download PDF

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CN114867852A
CN114867852A CN202080090503.6A CN202080090503A CN114867852A CN 114867852 A CN114867852 A CN 114867852A CN 202080090503 A CN202080090503 A CN 202080090503A CN 114867852 A CN114867852 A CN 114867852A
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Y·金
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Pairing Plant Service Co ltd
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04002—Adenine deaminase (3.5.4.2)

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  • General Health & Medical Sciences (AREA)
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  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202080090503.6A 2019-10-30 2020-10-30 V型crispr-cas碱基编辑器及其使用方法 Pending CN114867852A (zh)

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US201962927914P 2019-10-30 2019-10-30
US62/927,914 2019-10-30
PCT/US2020/058082 WO2021087182A1 (en) 2019-10-30 2020-10-30 Type v crispr-cas base editors and methods of use thereof

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EP (2) EP4051788A4 (https=)
JP (2) JP2023501223A (https=)
KR (1) KR20220110739A (https=)
CN (1) CN114867852A (https=)
AU (1) AU2020372931A1 (https=)
CA (1) CA3158897A1 (https=)
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CN115725533A (zh) * 2022-11-18 2023-03-03 中国农业科学院作物科学研究所 一种获得抗草甘膦水稻的方法及其所用双碱基融合编辑系统
WO2024125221A1 (zh) * 2022-12-15 2024-06-20 上海科技大学 一种碱基编辑系统及碱基编辑方法

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WO2023164722A1 (en) * 2022-02-28 2023-08-31 Pairwise Plants Services, Inc. Engineered crispr-cas effector proteins and methods of use thereof
WO2023250384A2 (en) * 2022-06-22 2023-12-28 The Regents Of The University Of California Crispr-cas effector polypeptides and methods of use thereof
WO2024158777A1 (en) * 2023-01-23 2024-08-02 The General Hospital Corporation Methods and compositions for inhibiting suppression of anti-tumor immunity by targeting ligand-receptor interactions present in the placenta
WO2025019273A1 (en) * 2023-07-14 2025-01-23 Epicrispr Biotechnologies, Inc. Engineered nucleases and guide rna spacer sequences therefor, and compositions, and methods of use thereof
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CN115725533A (zh) * 2022-11-18 2023-03-03 中国农业科学院作物科学研究所 一种获得抗草甘膦水稻的方法及其所用双碱基融合编辑系统
CN115725533B (zh) * 2022-11-18 2023-08-25 中国农业科学院作物科学研究所 一种获得抗草甘膦水稻的方法及其所用双碱基融合编辑系统
WO2024125221A1 (zh) * 2022-12-15 2024-06-20 上海科技大学 一种碱基编辑系统及碱基编辑方法

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EP4051788A4 (en) 2023-12-06
EP4051788A1 (en) 2022-09-07
EP4471149A2 (en) 2024-12-04
KR20220110739A (ko) 2022-08-09
AU2020372931A1 (en) 2022-05-12
US20210130827A1 (en) 2021-05-06
WO2021087182A1 (en) 2021-05-06
EP4471149A3 (en) 2025-03-05
JP2023501223A (ja) 2023-01-18
JP2025157268A (ja) 2025-10-15
NZ787352A (en) 2025-12-19

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