CN114854634A - Culture medium formula of bacillus - Google Patents
Culture medium formula of bacillus Download PDFInfo
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- CN114854634A CN114854634A CN202210538828.7A CN202210538828A CN114854634A CN 114854634 A CN114854634 A CN 114854634A CN 202210538828 A CN202210538828 A CN 202210538828A CN 114854634 A CN114854634 A CN 114854634A
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- 239000001963 growth medium Substances 0.000 title claims abstract description 112
- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 61
- 239000002994 raw material Substances 0.000 claims abstract description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 16
- 229920001817 Agar Polymers 0.000 claims abstract description 15
- 239000004254 Ammonium phosphate Substances 0.000 claims abstract description 15
- 239000001888 Peptone Substances 0.000 claims abstract description 15
- 108010080698 Peptones Proteins 0.000 claims abstract description 15
- 239000008272 agar Substances 0.000 claims abstract description 15
- 229910000148 ammonium phosphate Inorganic materials 0.000 claims abstract description 15
- 235000019289 ammonium phosphates Nutrition 0.000 claims abstract description 15
- 239000001506 calcium phosphate Substances 0.000 claims abstract description 15
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 15
- 235000011010 calcium phosphates Nutrition 0.000 claims abstract description 15
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000012153 distilled water Substances 0.000 claims abstract description 15
- GVALZJMUIHGIMD-UHFFFAOYSA-H magnesium phosphate Chemical compound [Mg+2].[Mg+2].[Mg+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O GVALZJMUIHGIMD-UHFFFAOYSA-H 0.000 claims abstract description 15
- 239000004137 magnesium phosphate Substances 0.000 claims abstract description 15
- 229910000157 magnesium phosphate Inorganic materials 0.000 claims abstract description 15
- 229960002261 magnesium phosphate Drugs 0.000 claims abstract description 15
- 235000010994 magnesium phosphates Nutrition 0.000 claims abstract description 15
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims abstract description 15
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims abstract description 15
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 15
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 15
- 235000019319 peptone Nutrition 0.000 claims abstract description 15
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims abstract description 15
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 14
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 14
- 241000196324 Embryophyta Species 0.000 claims description 36
- 229920002472 Starch Polymers 0.000 claims description 31
- 235000019698 starch Nutrition 0.000 claims description 31
- 239000008107 starch Substances 0.000 claims description 31
- 230000001954 sterilising effect Effects 0.000 claims description 18
- 238000010438 heat treatment Methods 0.000 claims description 17
- 244000017020 Ipomoea batatas Species 0.000 claims description 10
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 10
- 238000009472 formulation Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 9
- 230000001376 precipitating effect Effects 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 238000005303 weighing Methods 0.000 claims description 9
- 239000002609 medium Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 2
- 108010013639 Peptidoglycan Proteins 0.000 description 2
- WJJMNDUMQPNECX-UHFFFAOYSA-N dipicolinic acid Chemical compound OC(=O)C1=CC=CC(C(O)=O)=N1 WJJMNDUMQPNECX-UHFFFAOYSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 241001112741 Bacillaceae Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 108010062877 Bacteriocins Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 229940010556 ammonium phosphate Drugs 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 229960001714 calcium phosphate Drugs 0.000 description 1
- DLHXTEGKGWRZCW-UHFFFAOYSA-L calcium;pyridine-2-carboxylate Chemical compound [Ca+2].[O-]C(=O)C1=CC=CC=N1.[O-]C(=O)C1=CC=CC=N1 DLHXTEGKGWRZCW-UHFFFAOYSA-L 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 150000002772 monosaccharides Chemical group 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 241001446247 uncultured actinomycete Species 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a culture medium formula of bacillus, which comprises the following raw materials in parts by weight: 5-20 parts of peptone, 120-300 parts of yeast, 30-70 parts of agar, 30-70 parts of monopotassium phosphate, 35-65 parts of calcium phosphate, 3-7 parts of magnesium phosphate, 0.5-1.5 parts of ammonium phosphate, 0.4-2 parts of magnesium sulfate heptahydrate, 30-70 parts of sugar and 20000-30000 parts of distilled water. The bacillus cultured by the culture medium is excellent in cold resistance.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a culture medium formula of bacillus.
Background
The bacillus belongs to the family of bacillaceae and the genus bacillus, and is a gram-positive bacterium capable of producing resistant endospore, the cell is rod-shaped, and the outer layer is covered with a large amount of calcium dipicolinate. The cortex is positioned between the core and the spore shell, contains rich peptidoglycan, and the core is a highly concentrated and inert chromosome; the outer wall of the outermost layer is a peptidoglycan wall, and one or more layers of spore coat containing protein. The spores have a thick multi-layer structure with low water content, so the spores have strong refractivity, are not easy to color dyes, have good stability, are resistant to oxidation, extrusion and high temperature, can endure the high temperature of 60 ℃ for a long time, can survive for 20min at the temperature of 120 ℃, are acid-base resistant, and can keep activity in a gastric acid environment, which is probably related to the unique high-content dipicolinic acid of the spores. Meanwhile, the bacillus subtilis also has broad-spectrum bacillus activity, can produce bacteriocin and inhibit pathogenic bacteria. The antibacterial substance generated by the bacillus can prevent and treat various plant diseases, and certain bacillus biocontrol strains are commercialized or have limited commercial production application permission.
The culture medium is a nutrient medium prepared from different nutrient substances for the growth and reproduction of microorganisms, plants or animals. Generally, the food contains carbohydrate, nitrogen-containing substances, inorganic salt, vitamins, water and other substances. The culture medium is not only a basic substance for providing nutrition for the cells and promoting the proliferation of the cells, but also a living environment for the growth and the propagation of the cells.
The culture medium has many kinds, and can be divided into natural culture medium, synthetic culture medium and semi-synthetic culture medium according to the source of the preparation raw material; can be divided into solid culture medium, liquid culture medium and semisolid culture medium according to physical state; according to the culture function, the culture medium can be divided into a basic culture medium, a selective culture medium, an enriched culture medium, an identification culture medium and the like; according to the application range, the culture medium can be divided into a bacteria culture medium, an actinomycete culture medium, a yeast culture medium, a fungus culture medium and the like.
In the prior art, the cold resistance of bacillus cultured by a common culture medium is poor, the preservation time of the bacillus is shortened when the temperature in the culture medium is reduced, and the activity of the bacillus is reduced after the bacillus is produced under the same storage time.
Disclosure of Invention
The invention aims to provide a culture medium formula of bacillus, which is used for solving the technical problem of poor cold resistance of bacillus cultured by a common culture medium in the prior art.
In order to achieve the above purpose, an embodiment of the present invention provides a culture medium formula of bacillus, which comprises the following raw materials by weight:
5 to 20 parts of peptone, 120 to 300 parts of yeast, 30 to 70 parts of agar,
30 to 70 parts of monopotassium phosphate, 35 to 65 parts of calcium phosphate, 3 to 7 parts of magnesium phosphate,
0.5 to 1.5 parts of ammonium phosphate, 0.4 to 2 parts of magnesium sulfate heptahydrate, 30 to 70 parts of sugar,
20000 to 30000 parts of distilled water.
In one preferable scheme of the invention, the bacillus culture medium formula comprises the following raw materials in parts by weight:
7-16 parts of peptone, 150-260 parts of yeast, 40-63 parts of agar,
38 to 60 portions of monopotassium phosphate, 43 to 59 portions of calcium phosphate, 4 to 7 portions of magnesium phosphate,
0.7 to 1.4 portions of ammonium phosphate, 0.6 to 1.5 portions of magnesium sulfate heptahydrate, 36 to 65 portions of sugar,
23000-28000 parts of distilled water.
In a preferred scheme of the invention, the bacillus culture medium formula comprises the following raw materials in parts by weight:
8-13 parts of peptone, 180-230 parts of yeast, 45-56 parts of agar,
43-55 parts of monopotassium phosphate, 47-54 parts of calcium phosphate, 3-6 parts of magnesium phosphate,
0.8 to 1.2 parts of ammonium phosphate, 0.6 to 1.3 parts of magnesium sulfate heptahydrate, 46 to 56 parts of sugar,
2450-26300 parts of distilled water.
In a preferred embodiment of the present invention, the sugar is a plant sugar, the plant sugar is specifically starch obtained by crushing, filtering and precipitating sweet potatoes, and the starch is mixed with water to form the plant sugar.
In one preferable embodiment of the present invention, the content of starch is 20 to 40 parts.
In a preferred embodiment of the present invention, the method for preparing the culture medium formulation of bacillus comprises the following steps:
step (1): preparing raw materials: weighing the raw materials in parts by weight respectively;
step (2): preparing a culture medium: adding the raw materials into a container and uniformly stirring;
and (3): adjusting the pH value: adjusting the pH of the culture medium prepared in the step (2);
and (4): and (3) sterilization: sterilizing the culture medium obtained in the step (3).
In one preferred embodiment of the present invention, in the step (2), the heating temperature is 100 ℃ to 120 ℃.
In a preferred embodiment of the present invention, in the step (3), the pH of the medium is 7.0 to 7.4.
In a preferred embodiment of the present invention, in step (4), the medium is sterilized by high temperature and high pressure.
In conclusion, the beneficial effects of the invention are as follows:
1. the bacillus cultured by the culture medium formula has the characteristic of excellent cold resistance. Compared with the bacillus cultured by a common culture medium, the bacillus cultured by the culture medium has obviously higher activity after being produced and stored for the same time.
2. The bacillus cultured by the culture medium of the invention has prolonged preservation time when the temperature of the culture medium is reduced.
3. The culture medium is added with starch to replace glucose, and the used starch is extracted from sweet potatoes, so that the production cost of the culture medium is reduced, and the economic benefit of enterprises is improved; meanwhile, because the glucose is monosaccharide and the starch is polysaccharide, the starch provides more nutrient substances for the bacillus in the culture medium, thereby prolonging the survival time of the bacillus.
Detailed Description
Example 1
A culture medium formula of bacillus comprises the following raw materials in parts by weight: 12 parts of peptone, 201 parts of yeast, 50 parts of agar, 52 parts of monopotassium phosphate, 51 parts of calcium phosphate, 5 parts of magnesium phosphate, 1 part of ammonium phosphate, 1.1 parts of magnesium sulfate heptahydrate, 50 parts of sugar and 25000 parts of distilled water;
wherein the sugar is plant sugar, the plant sugar is specifically starch obtained by crushing, filtering and precipitating sweet potatoes, and the obtained starch is mixed with water to form the plant sugar, and the adding amount of the starch in the plant sugar is 30 parts.
The preparation method of the culture medium formula of the bacillus comprises the following steps:
step (1): preparing raw materials: weighing the raw materials in parts by weight respectively;
step (2): preparing a culture medium: adding the raw materials into a container, heating and uniformly stirring, wherein the heating temperature is 100-120 ℃;
and (3): adjusting the pH value: adjusting the pH value of the culture medium prepared in the step (2), wherein the pH value of the adjusted culture medium is 7.0-7.4;
and (4): and (3) sterilization: and (4) sterilizing the culture medium obtained in the step (3) at high temperature and high pressure for 20min, wherein the temperature is 120-130 ℃.
Adding bacillus into the prepared culture medium for culturing, wherein the culture temperature is controlled to be 20-40 ℃.
Example 2
A culture medium formula of bacillus comprises the following raw materials in parts by weight: 5 parts of peptone, 300 parts of yeast, 30 parts of agar, 70 parts of monopotassium phosphate, 35 parts of calcium phosphate, 7 parts of magnesium phosphate, 1.5 parts of ammonium phosphate, 0.4 part of magnesium sulfate heptahydrate, 30 parts of sugar and 20000 parts of distilled water;
wherein the sugar is plant sugar, the plant sugar is specifically starch obtained by crushing, filtering and precipitating sweet potatoes, and the obtained starch is mixed with water to form the plant sugar, and the adding amount of the starch in the plant sugar is 20 parts.
The preparation method of the culture medium formula of the bacillus comprises the following steps:
step (1): preparing raw materials: weighing the raw materials in parts by weight respectively;
step (2): preparing a culture medium: adding the raw materials into a container, heating and uniformly stirring, wherein the heating temperature is 100-120 ℃;
and (3): adjusting the pH value: adjusting the pH value of the culture medium prepared in the step (2), wherein the pH value of the adjusted culture medium is 7.0-7.4;
and (4): and (3) sterilization: and (4) sterilizing the culture medium obtained in the step (3) at high temperature and high pressure for 20min, wherein the temperature is 120-130 ℃.
Adding bacillus into the prepared culture medium for culturing, wherein the culture temperature is controlled to be 20-40 ℃.
Example 3
A culture medium formula of bacillus comprises the following raw materials in parts by weight: 20 parts of peptone, 120 parts of yeast, 70 parts of agar, 30 parts of monopotassium phosphate, 65 parts of calcium phosphate, 3 parts of magnesium phosphate, 0.5 part of ammonium phosphate, 2 parts of magnesium sulfate heptahydrate, 70 parts of sugar and 30000 parts of distilled water;
wherein the sugar is plant sugar, the plant sugar is specifically starch obtained by crushing, filtering and precipitating sweet potatoes, and the obtained starch is mixed with water to form the plant sugar, and the adding amount of the starch in the plant sugar is 40 parts.
The preparation method of the culture medium formula of the bacillus comprises the following steps:
step (1): preparing raw materials: weighing the raw materials in parts by weight respectively;
step (2): preparing a culture medium: adding the raw materials into a container, heating and uniformly stirring, wherein the heating temperature is 100-120 ℃;
and (3): adjusting the pH value: adjusting the pH value of the culture medium prepared in the step (2), wherein the pH value of the adjusted culture medium is 7.0-7.4;
and (4): and (3) sterilization: and (4) sterilizing the culture medium obtained in the step (3) at high temperature and high pressure for 20min, wherein the temperature is 120-130 ℃.
Adding bacillus into the prepared culture medium for culturing, wherein the culture temperature is controlled to be 20-40 ℃.
Example 4
A culture medium formula of bacillus comprises the following raw materials in parts by weight: 7 parts of peptone, 150 parts of yeast, 40 parts of agar, 60 parts of monopotassium phosphate, 43 parts of calcium phosphate, 4 parts of magnesium phosphate, 0.7 part of ammonium phosphate, 1.5 parts of magnesium sulfate heptahydrate, 36 parts of sugar and 23000 parts of distilled water;
wherein the sugar is plant sugar, the plant sugar is specifically starch obtained by crushing, filtering and precipitating sweet potatoes, and the obtained starch is mixed with water to form the plant sugar, and the adding amount of the starch in the plant sugar is 26 parts.
The preparation method of the culture medium formula of the bacillus comprises the following steps:
step (1): preparing raw materials: weighing the raw materials in parts by weight respectively;
step (2): preparing a culture medium: adding the raw materials into a container, heating and uniformly stirring, wherein the heating temperature is 100-120 ℃;
and (3): adjusting the pH value: adjusting the pH value of the culture medium prepared in the step (2), wherein the pH value of the adjusted culture medium is 7.0-7.4;
and (4): and (3) sterilization: and (4) sterilizing the culture medium obtained in the step (3) at high temperature and high pressure for 20min, wherein the temperature is 120-130 ℃.
Adding bacillus into the prepared culture medium for culturing, wherein the culture temperature is controlled to be 20-40 ℃.
Example 5
A culture medium formula of bacillus comprises the following raw materials in parts by weight: 16 parts of peptone, 260 parts of yeast, 63 parts of agar, 38 parts of monopotassium phosphate, 59 parts of calcium phosphate, 6 parts of magnesium phosphate, 1.4 parts of ammonium phosphate, 0.6 part of magnesium sulfate heptahydrate, 65 parts of sugar and 28000 parts of distilled water;
wherein the sugar is plant sugar, the plant sugar is specifically starch obtained by crushing, filtering and precipitating sweet potatoes, and the obtained starch is mixed with water to form the plant sugar, and the adding amount of the starch in the plant sugar is 37 parts.
The preparation method of the culture medium formula of the bacillus comprises the following steps:
step (1): preparing raw materials: weighing the raw materials in parts by weight respectively;
step (2): preparing a culture medium: adding the raw materials into a container, heating and uniformly stirring, wherein the heating temperature is 100-120 ℃;
and (3): adjusting the pH value: adjusting the pH value of the culture medium prepared in the step (2), wherein the pH value of the adjusted culture medium is 7.0-7.4;
and (4): and (3) sterilization: and (4) sterilizing the culture medium obtained in the step (3) at high temperature and high pressure for 20min, wherein the temperature is 120-130 ℃.
Adding bacillus into the prepared culture medium for culturing, wherein the culture temperature is controlled to be 20-40 ℃.
Example 6
A culture medium formula of bacillus comprises the following raw materials in parts by weight: 13 parts of peptone, 230 parts of yeast, 45 parts of agar, 55 parts of monopotassium phosphate, 54 parts of calcium phosphate, 3 parts of magnesium phosphate, 0.8 part of ammonium phosphate, 1.3 parts of magnesium sulfate heptahydrate, 46 parts of sugar and 2450 parts of distilled water;
wherein the sugar is plant sugar, the plant sugar is specifically starch obtained by crushing, filtering and precipitating sweet potatoes, and the obtained starch is mixed with water to form the plant sugar, and the adding amount of the starch in the plant sugar is 23 parts.
The preparation method of the culture medium formula of the bacillus comprises the following steps:
step (1): preparing raw materials: weighing the raw materials in parts by weight respectively;
step (2): preparing a culture medium: adding the raw materials into a container, heating and uniformly stirring, wherein the heating temperature is 100-120 ℃;
and (3): adjusting the pH value: adjusting the pH value of the culture medium prepared in the step (2), wherein the pH value of the adjusted culture medium is 7.0-7.4;
and (4): and (3) sterilization: and (4) sterilizing the culture medium obtained in the step (3) at high temperature and high pressure for 20min, wherein the temperature is 120-130 ℃.
Adding bacillus into the prepared culture medium for culturing, wherein the culture temperature is controlled to be 20-40 ℃.
Example 7
A culture medium formula of bacillus comprises the following raw materials in parts by weight: 8 parts of peptone, 180 parts of yeast, 56 parts of agar, 543 parts of monopotassium phosphate, 47 parts of calcium phosphate, 6 parts of magnesium phosphate, 1.2 parts of ammonium phosphate, 0.6 part of magnesium sulfate heptahydrate, 56 parts of sugar and 26300 parts of distilled water;
wherein the sugar is plant sugar, the plant sugar is specifically starch obtained by crushing, filtering and precipitating sweet potatoes, and the obtained starch is mixed with water to form the plant sugar, and the adding amount of the starch in the plant sugar is 32 parts.
The preparation method of the culture medium formula of the bacillus comprises the following steps:
step (1): preparing raw materials: weighing the raw materials in parts by weight respectively;
step (2): preparing a culture medium: adding the raw materials into a container, heating and uniformly stirring, wherein the heating temperature is 100-120 ℃;
and (3): adjusting the pH value: adjusting the pH value of the culture medium prepared in the step (2), wherein the pH value of the adjusted culture medium is 7.0-7.4;
and (4): and (3) sterilization: and (4) sterilizing the culture medium obtained in the step (3) at high temperature and high pressure for 20min, wherein the temperature is 120-130 ℃.
Adding bacillus into the prepared culture medium for culturing, wherein the culture temperature is controlled to be 20-40 ℃.
In conclusion, the culture medium prepared from peptone, yeast, agar, potassium dihydrogen phosphate, calcium phosphate, magnesium phosphate, ammonium phosphate, magnesium sulfate heptahydrate, sugar and distilled water has excellent cold resistance, and compared with the bacillus cultured by a common culture medium, the activity of the bacillus cultured by the culture medium is obviously higher than that of the bacillus cultured by the common culture medium after the same storage time.
Claims (9)
1. The culture medium formula of bacillus is characterized in that: the material comprises the following raw materials in parts by weight:
5 to 20 parts of peptone, 120 to 300 parts of yeast, 30 to 70 parts of agar,
30 to 70 parts of monopotassium phosphate, 35 to 65 parts of calcium phosphate, 3 to 7 parts of magnesium phosphate,
0.5 to 1.5 parts of ammonium phosphate, 0.4 to 2 parts of magnesium sulfate heptahydrate, 30 to 70 parts of sugar,
20000 to 30000 parts of distilled water.
2. The culture medium formulation of bacillus according to claim 1, wherein: the bacillus culture medium formula comprises the following raw materials in parts by weight:
7-16 parts of peptone, 150-260 parts of yeast, 40-63 parts of agar,
38 to 60 portions of monopotassium phosphate, 43 to 59 portions of calcium phosphate, 4 to 7 portions of magnesium phosphate,
0.7 to 1.4 portions of ammonium phosphate, 0.6 to 1.5 portions of magnesium sulfate heptahydrate, 36 to 65 portions of sugar,
23000-28000 parts of distilled water.
3. The medium formulation of claim 1, wherein the medium formulation comprises: the bacillus culture medium formula comprises the following raw materials in parts by weight:
8-13 parts of peptone, 180-230 parts of yeast, 45-56 parts of agar,
43-55 parts of monopotassium phosphate, 47-54 parts of calcium phosphate, 3-6 parts of magnesium phosphate,
0.8 to 1.2 parts of ammonium phosphate, 0.6 to 1.3 parts of magnesium sulfate heptahydrate, 46 to 56 parts of sugar,
2450-26300 parts of distilled water.
4. The culture medium formulation of bacillus according to claim 1, wherein: the sugar is plant sugar, the plant sugar is starch obtained by crushing, filtering and precipitating sweet potatoes, and the starch is mixed with water to form the plant sugar.
5. The culture medium formulation of bacillus according to claim 4, wherein: the content of the starch is 20-40 parts.
6. The culture medium formulation of bacillus according to claim 1, wherein: the preparation method of the bacillus culture medium formula comprises the following steps:
step (1): preparing raw materials: weighing the raw materials in parts by weight respectively;
step (2): preparing a culture medium: adding the raw materials into a container, heating and uniformly stirring;
and (3): adjusting the pH value: adjusting the pH of the culture medium prepared in the step (2);
and (4): and (3) sterilization: sterilizing the culture medium obtained in the step (3).
7. The culture medium formulation of bacillus according to claim 6, wherein: in the step (2), the heating temperature is 100-120 ℃.
8. The culture medium formulation of bacillus according to claim 6, wherein: in the step (3), the pH value of the culture medium is 7.0-7.4.
9. The culture medium formulation of bacillus according to claim 6, wherein: in the step (4), the culture medium is sterilized by high temperature and high pressure.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004275048A (en) * | 2003-03-14 | 2004-10-07 | Rikogaku Shinkokai | Dna and protein controlling number or size of plastid |
| CN104560783A (en) * | 2014-12-05 | 2015-04-29 | 广西大学 | Bacillus subtilis enrichment culture medium and application thereof |
| CN105018397A (en) * | 2015-08-28 | 2015-11-04 | 济南舜昊生物科技有限公司 | Bacillus subtilis culture medium and preparation method thereof |
| CN107119005A (en) * | 2017-05-11 | 2017-09-01 | 张维秀 | A kind of bafillus natto enhancer of proliferation and preparation method thereof |
| CN110462021A (en) * | 2017-01-26 | 2019-11-15 | 拜耳作物科学有限合伙公司 | Promote the method for bacillus spore germination |
-
2022
- 2022-05-18 CN CN202210538828.7A patent/CN114854634A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004275048A (en) * | 2003-03-14 | 2004-10-07 | Rikogaku Shinkokai | Dna and protein controlling number or size of plastid |
| CN104560783A (en) * | 2014-12-05 | 2015-04-29 | 广西大学 | Bacillus subtilis enrichment culture medium and application thereof |
| CN105018397A (en) * | 2015-08-28 | 2015-11-04 | 济南舜昊生物科技有限公司 | Bacillus subtilis culture medium and preparation method thereof |
| CN110462021A (en) * | 2017-01-26 | 2019-11-15 | 拜耳作物科学有限合伙公司 | Promote the method for bacillus spore germination |
| US20200024207A1 (en) * | 2017-01-26 | 2020-01-23 | Bayer Cropscience Lp | Method of promoting bacillus spore germination |
| CN107119005A (en) * | 2017-05-11 | 2017-09-01 | 张维秀 | A kind of bafillus natto enhancer of proliferation and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王继华 等: "一株拮抗链格孢(Alter...spp.)抗生素产生菌研究", 《哈尔滨工业大学学报》, vol. 38, no. 9, pages 1594 - 1596 * |
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