CN114831197A

CN114831197A - Composite dark tea and preparation method and application thereof

Info

Publication number: CN114831197A
Application number: CN202210559559.2A
Authority: CN
Inventors: 文驰; 沈程文; 叶孟柯; 程显淯; 王铭涵; 谢芳楠; 徐超富; 刘仲华
Original assignee: Hunan Chuming Tea Industry Co ltd
Current assignee: Hunan Chuming Tea Industry Co ltd
Priority date: 2022-05-23
Filing date: 2022-05-23
Publication date: 2022-08-02
Anticipated expiration: 2042-05-23
Also published as: CN114831197B

Abstract

The invention provides a composite dark tea and a preparation method and application thereof, belonging to the technical field of food and medicine, wherein the composite dark tea comprises the following components in parts by weight: 50-75 parts of golden flower poria brick dark tea, 9-18 parts of broadleaf holly leaf, 5-15 parts of momordica grosvenori and 0-36 parts of black tea and/or green tea. The compound black tea has small dosage, can remarkably reduce the weight, blood sugar, body fat rate, blood fat and liver fat of a high-fat diet mouse, improves insulin sensitivity, and has remarkable effects on improving the health level under the high-fat diet condition and preventing obesity.

Description

Composite dark tea and preparation method and application thereof

Technical Field

The invention belongs to the technical field of food and medicine, and particularly relates to a composite dark tea and a preparation method and application thereof.

Background

As is known to all, obesity is the basis of the onset of various chronic diseases such as diabetes, cardiovascular diseases and the like, and one of the most important reasons of obesity is high-fat diet, 25-30 g of cooking oil recommended by the world health organization and Chinese nutritional dietary guidelines per person every day, and 25% -30% of fat energy supply, but the current actual cooking oil in China reaches 42.1 g per person every day, and the dietary fat energy supply ratio of urban groups and some of the more developed regions and rural residents in villages and towns reaches 35%.

The Fuzhuan black tea has the characteristics of pure fragrance, mellow taste, red yellow and bright soup color and the like due to the growth of eurotium cristatum (golden flower) of the Fuzhuan black tea, but has hunger sensation in the stomach and intestine after drinking; the broadleaf holly leaf tea is a tea-like beverage prepared from medicinal and edible broadleaf holly root, is bitter and sweet in taste, has the effects of diminishing inflammation, relieving summer-heat, promoting fluid production, quenching thirst, helping digestion, reducing phlegm and the like, and has the defects that the traditional broadleaf holly leaf tea is extremely bitter in taste and is unwilling to drink by most people in life practice. The Fuzhuan dark tea and the broadleaf holly leaf have lipid-lowering effects, but the Fuzhuan dark tea and the broadleaf holly leaf can achieve remarkable lipid-lowering effects after being drunk at high dose or extracted with effective components and concentrated, and researches show that people with weight of 60KG can achieve remarkable lipid-lowering effects only by drinking 180-360 g of the Fuzhuan dark tea or 20-60 g of the broadleaf holly leaf every day, and the Fuzhuan dark tea and the broadleaf holly leaf are difficult to apply for a long time in life. The momordica grosvenori has high nutritional value, has the effects of clearing away summer-heat, eliminating phlegm and stopping cough, clearing lung and moistening intestines and the like, contains momordica grosvenori sweet glycosides which are 300 times sweeter than cane sugar, and does not generate heat.

At present, under the condition of supplying energy to 35% of fat of residents in China, the tea drink prepared by the combined application of the Fuzhuan dark tea, the broadleaf holly leaf, the grosvenor momordica fruit and other tea is not reported in relevant researches on preventing obesity, reducing liver fat deposition and improving blood sugar homeostasis.

Disclosure of Invention

The compound black tea provided by the invention is small in dosage, can obviously reduce the weight, blood sugar, body fat rate, blood fat and liver fat of high-fat mice, improves insulin sensitivity, and has obvious effects of improving health level under high-fat diet conditions and preventing obesity.

In order to solve the technical problems, the invention provides the following technical scheme:

the invention provides a composite dark tea which comprises the following components in parts by weight: 50-75 parts of golden flower poria brick dark tea, 9-18 parts of broadleaf holly leaf, 5-15 parts of momordica grosvenori and 0-36 parts of black tea and/or green tea.

Preferably, the composite dark tea comprises the following components in parts by weight: 60-65 parts of golden flower poria brick dark tea, 10-15 parts of broadleaf holly leaf, 7.5-10 parts of momordica grosvenori and 10-22.5 parts of black tea and/or green tea.

Preferably, the black tea comprises any one or more of Yinghong No. 9, Dian red, Qimen black tea and Jinjunmei.

Preferably, the green tea comprises one or more of gold green tea, ancient navy tippy, dragon well and Biluochun tea.

The invention provides a preparation method of the composite dark tea, which comprises the following steps: respectively pulverizing the golden flower poria brick dark tea, the broadleaf holly leaf, the momordica grosvenori, the black tea and/or the green tea, and mixing to obtain the composite dark tea.

Preferably, the size of the crushed powder is 10-40 meshes.

Preferably, the subpackage amount of the composite dark tea is 1.5-3 g per bag.

The invention provides application of the compound dark tea in preparing foods or medicines for improving high fat diet.

The invention also provides application of the composite dark tea in preparing anti-obesity food or drugs.

Preferably, the application mode is as follows: 1-4 bags are taken every day, and each bag is brewed with 250-650 ml of boiling water and is freely drunk after standing for 2-5 minutes.

Compared with the prior art, the invention has the following beneficial effects:

(1) under the condition of high fat (35% of energy is supplied to fat), the composite dark tea can obviously reduce the weight, the body fat rate, the blood fat and the liver fat, improve the insulin sensitivity and reduce the blood sugar after being drunk for a long time, and has obvious effects on improving the health level under the condition of high fat diet and preventing obesity.

(2) The composite black tea has good taste, is slightly bitter before sweet after entering the mouth, moistens the throat and quenches thirst, and overcomes the defects of hunger sensation, extremely bitter flavor of broadleaf holly leaves and the like of the traditional Fu brick black tea after being drunk.

(3) The compound dark tea has small dosage, and solves the technical problems that the traditional dark tea and broadleaf holly leaf can achieve the effects of reducing blood fat, reducing blood sugar and resisting obesity only by drinking high dosage.

(4) The composite dark tea is simple and convenient in preparation process, convenient to drink, and only needs to be brewed, and is suitable for long-term use in places such as families, offices and the like.

Drawings

Figure 1 effect of composite dark tea on body weight of mice on high fat diet.

FIG. 2 shows the effect of black tea on the final body weight, body fat percentage and food consumption of mice with high-fat diet.

FIG. 3 shows the effect of black tea on perirenal, epididymal, subcutaneous and mesenteric fat coefficients in mice with high-fat diet.

FIG. 4H & E staining pattern (200) of subcutaneous adipose tissue of mice with high fat diet by composite dark tea.

FIG. 5H & E staining pattern of complex dark tea on brown adipose tissue of high-fat diet mice (X200).

FIG. 6 shows the effect of black tea on fasting plasma glucose and glucose tolerance in mice with high-fat diet.

FIG. 7H & E staining pattern (200) of complex dark tea on liver lipid deposition in mice with high fat diet.

FIG. 8 is a graph of oil red O staining (200) of liver lipid deposition of mice with high fat diet by composite dark tea.

FIG. 9 percentage of the area of oil red O staining of liver tissue of mice with high fat diet by composite dark tea.

Note: # indicates that the HFD group was significantly different from the ND group (P <0.05), # indicates that the HFD group was significantly different from the ND group (P <0.01), # indicates that the drinking complex black tea group was significantly different from the HFD group (P <0.05), and # indicates that the drinking complex black tea group was significantly different from the HFD group (P < 0.01).

Figure 10 effect of different concentrations of multiple aqueous ilex latifolia interventions on mouse body weight.

FIG. 11 influence of Compound folium Ilicis on body fat percentage of mice with high fat diet

FIG. 12 shows the effect of Folum Ilicis on perirenal, epididymal, subcutaneous and mesenteric fat coefficients in mice with high fat diet.

FIG. 13H & E staining pattern (200) of composite Folum Ilicis on subcutaneous fat of high fat diet mice.

FIG. 14H & E staining pattern (200) of composite Folum Ilicis on brown fat of mice with high fat diet.

FIG. 15 shows the effect of Kuding tea on fasting blood glucose and glucose tolerance of mice.

FIG. 16H & E staining pattern (200) of liver lipid deposition of high fat diet mice with composite Folum Ilicis.

FIG. 17 oil red O staining pattern (200) of composite Folum Ilicis on liver lipid deposition in high fat diet mice.

Note: compared with the high-fat control group, the difference is significant (P <0.05), and the difference is very significant (P < 0.01).

Detailed Description

The invention provides a composite dark tea which comprises the following components in parts by weight: 50-75 parts of golden flower poria brick dark tea, 9-18 parts of broadleaf holly leaf, 5-15 parts of momordica grosvenori and 0-36 parts of black tea and/or green tea; preferably, the golden flower brick dark tea comprises 60-65 parts of golden flower brick dark tea, 10-15 parts of broadleaf holly leaf, 7.5-10 parts of momordica grosvenori and 10-22.5 parts of black tea and/or green tea. In the present invention, all the raw material components are commercially available products well known to those skilled in the art unless otherwise specified. According to the invention, through the synergistic effect of specific components, the composite dark tea can still remarkably reduce the weight, blood sugar, body fat rate, blood fat and liver fat of a high-fat diet mouse on the basis of less dosage than that of golden flower fu brick dark tea or broadleaf holly leaf, improve insulin sensitivity, and has remarkable effects on improving the health level under the high-fat diet condition and resisting obesity.

In the present invention, the black tea preferably includes any one or more of enghong No. 9, Yunnan red, Qimen black tea, Jinjunmei, more preferably enghong No. 9. The green tea preferably comprises one or more of gold green tea, ancient husuo tippy, dragon well and Biluochun tea, and more preferably gold green tea. The source of the compound tea is not particularly limited in the present invention, and commercially available products known to those skilled in the art may be used.

The invention provides a preparation method of the composite dark tea, which comprises the following steps: respectively pulverizing the golden flower poria brick dark tea, the broadleaf holly leaf, the momordica grosvenori, the black tea and/or the green tea, mixing and subpackaging to obtain the composite dark tea.

In the present invention, the size of the pulverization is preferably 10 to 40 mesh. In the invention, the golden flower dark tea is preferably finished dark tea which is entrusted, customized and processed into 1 kg of each piece, and is preferably crushed to 18-36 meshes, more preferably 24 meshes for later use; preferably crushing the broadleaf holly leaves to 18-36 meshes, more preferably to 24 meshes for later use; the fructus momordicae is preferably crushed to 12-24 meshes, and more preferably 18 meshes for later use; the black tea and/or the green tea is preferably ground to 18-36 meshes, and more preferably to 24 meshes.

In the invention, the subpackage amount of the composite dark tea is preferably 1.5-3 g/bag, and more preferably 1.8-2 g/bag.

The invention provides application of the composite dark tea in preparing food or medicine for improving high fat diet.

In the present invention, the application mode is preferably: 1-4 bags are taken every day, and each bag is brewed with 250-650 ml of boiling water, kept stand for 2-5 minutes and then drunk freely. In the invention, the population with normal weight (the body mass index BMI female <24, male <25) is 1-2 bags per day, each bag is brewed with 400-600 ml of boiling water, and the drinking water is freely drunk after standing for 2-5 minutes; 2-3 bags of overweight people (with a body mass index BMI of 24-28) are brewed with 400-500 ml of boiling water every day, and the overweight people can drink freely after standing for 5 minutes; the weight index BMI of obese people (BMI >28) is 3-4 bags per day, each bag is brewed with 300-500 ml of boiling water, and the obese people can drink freely after standing for 5 minutes.

The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The composite dark tea comprises the following components in parts by weight: 50 parts of golden flower poria brick dark tea, 9 parts of broadleaf holly leaf, 5 parts of momordica grosvenori and 36 parts of English red No. 9.

The preparation method of the composite dark tea comprises the following steps: pulverizing the golden flower fu brick dark tea into 24 meshes, pulverizing the broadleaf holly leaf into 24 meshes, pulverizing the grosvenor momordica into 18 meshes, and pulverizing the English red No. 9 into 24 meshes; mixing the crushed golden flower fu brick dark tea, the broadleaf holly leaf, the momordica grosvenori and the Yinghong No. 9 according to the weight parts, uniformly stirring, and subpackaging into 3g per bag.

Example 2

The composite dark tea comprises the following components in parts by weight: 70 parts of golden flower poria brick dark tea, 15 parts of broadleaf holly leaf and 15 parts of momordica grosvenori.

The preparation method of the composite dark tea comprises the following steps: pulverizing the golden flower fu brick dark tea into 40 meshes, pulverizing the broadleaf holly leaf into 10 meshes, and pulverizing the grosvener siraitia into 12 meshes; mixing the crushed golden flower fu brick dark tea, the broadleaf holly leaf and the momordica grosvenori according to the weight parts, uniformly stirring, and subpackaging to 1.5g per bag.

Example 3

The composite dark tea comprises the following components in parts by weight: 60 parts of golden flower poria brick dark tea, 10 parts of broadleaf holly leaf, 7.5 parts of momordica grosvenori and 20 parts of golden tea.

The preparation method of the composite dark tea comprises the following steps: pulverizing the golden flower fu brick dark tea into 30 meshes, pulverizing the broadleaf holly leaf into 24 meshes, pulverizing the grosvener siraitia into 12 meshes, and pulverizing the golden tea into 30 meshes; mixing the crushed golden flower poria brick dark tea, broadleaf holly leaf, momordica grosvenori and golden tea according to the weight parts, uniformly stirring, and subpackaging into 2g per bag.

Example 4

The composite dark tea comprises the following components in parts by weight: 65 parts of golden flower fu brick dark tea, 12.5 parts of broadleaf holly leaf, 10 parts of grosvenor momordica fruit and 22.5 parts of English red No. 9.

The preparation method of the composite dark tea comprises the following steps: pulverizing the golden flower fu brick dark tea into 24 meshes, pulverizing the broadleaf holly leaf into 24 meshes, pulverizing the grosvenor momordica into 18 meshes, and pulverizing the English red No. 9 into 24 meshes; mixing the crushed golden flower fu brick dark tea, the broadleaf holly leaf, the momordica grosvenori and the Yinghong No. 9 according to the weight parts, uniformly stirring, and subpackaging into 2g per bag.

Comparative example 1

Compared with the components in the embodiment 1, the components in parts by weight do not contain the golden flower fu brick dark tea, and the rest are the same.

Compared with the preparation method in the example 1, the preparation method does not contain the treatment of the golden flower Fu brick dark tea, and the other preparation methods are the same.

Comparative example 2

A composite broadleaf holly leaf comprises the following components in parts by weight: 65 parts of golden green tea, 12.5 parts of broadleaf holly leaf, 10 parts of momordica grosvenori and 22.5 parts of Yinghong No. 9.

Compared with the preparation method of the embodiment 4, the method for crushing the golden flower fu brick dark tea into 24 meshes is changed into the method for crushing the golden green tea into 24 meshes (or directly applying the crushed tea to pass through a 24-mesh sieve), and the rest is the same.

Example 5

The actual oil level (35% of fat energy supply) and the oil intake habit (50% of compound oil fat in each of lard and soybean oil) of Chinese residents are simulated, male C57BL/6J mice of 6 weeks are used as animal models, and a low-fat blank group (ND, 15% of fat energy supply, free drinking water), a high-fat feed control group (HFD, 35% of fat energy supply, free drinking water), a high-fat + compound black tea normal dose group (HL, 35% of fat energy supply, 6mg/ml of compound black tea prepared in example 4, free drinking tea), a high-fat + compound black tea strong tea group (HH, 35% of fat energy supply, 12mg/ml of compound black tea prepared in example 4, free drinking tea) are set for 18 weeks.

The body weights of the mice in each group were measured weekly during feeding and the results are shown in FIG. 1.

The body fat percentage and food intake of each group of mice after 18 weeks of feeding were measured, the neck of the mice after 18 weeks of feeding was cut, perirenal, epididymal, subcutaneous, and mesenteric fats of the mice were isolated, and the fat coefficients were calculated from the weight of each fat/weight, respectively, and the results are shown in fig. 2 and fig. 3.

As shown in fig. 1 and 2, the final body weight of the HFD mice was increased by 25% compared to the low-fat blank group (ND) without affecting food consumption, which was significantly higher than that of the blank control group (P < 0.01); compared with the HFD group, the body weight of the mice in the HL and HH groups is reduced by 15 percent and 16 percent respectively, and the difference is very obvious (P < 0.01); the body fat rates of HL and HH groups were reduced by 44% and 38%, respectively, with significant difference (P < 0.05). The research proves that the drinking of the composite dark tea has the functions of remarkably reducing the body weight and the body fat of the obesity caused by high-fat diet, and particularly the body fat rate of the normal-dose tea group is lower.

As shown in fig. 3, the perirenal, epididymal, subcutaneous inguinal, and mesenteric fats of the HL and HH groups were significantly reduced in fat factors (fat/body weight ratio) compared to the HFD group, indicating that drinking dark tea can reduce abdominal obesity caused by visceral fat deposition.

Subcutaneous inguinal adipose tissues and scapular region brown adipose tissues of the above-mentioned neck-broken sacrificed mice were peeled off, and subcutaneous inguinal adipose tissues and scapular region brown adipose tissues of the respective groups of mice after feeding for 18 weeks were observed using conventional H & E staining, and the results were shown in fig. 4 and 5.

As shown in fig. 4, the effect of composite dark tea on the subcutaneous inguinal adipocyte morphology and adipocyte size of the high fat diet mice: the blank control group (ND) fat cells have regular shapes, clear cell outlines and relatively consistent sizes. The fat cells of the High Fat Diet (HFD) are remarkably increased, the cells are different in size and irregular in shape, and the intersection of the cells are fused, so that the cells tend to be fused into large cells. In the HL and HH groups, the size of the fat cells was reduced, the morphology was relatively regular, and the tendency of the fat cells to fuse into large cells was reduced compared with the HFD group.

As shown in fig. 5, the effect of composite dark tea on the morphology and size of brown adipocytes in mice with high-fat diet: the blank control group (ND) brown fat cells have regular shapes and relatively consistent sizes. The number of fat droplets of brown fat cells in a High Fat Diet (HFD) group is remarkably increased, the size of fat cells is remarkably increased, the cells are inconsistent in size and irregular in shape, and the intersection parts of the cells are fused, so that the cells tend to be fused into large cells. The results of the HL and HH groups comparing with the HFD group, the fat drop number is reduced obviously, the size of the fat cell is reduced, the shape is relatively regular, the tendency of fusing into the large cell is in a slowing state, and the results show that the excessive accumulation of the fat tissue and the abnormal enlargement of the fat cell of the mouse caused by the high fat diet can be obviously changed by drinking the composite black tea.

Mouse OGTT test: after feeding for 18 weeks, the mice in each group were fasted for 10h, and then glucose gavage dose was calculated according to the fasting body weight, fasting blood glucose (0min) was measured, glucose gavage was performed at a dose of 3g/kg body weight of glucose (i.e. 0.0075mL/g body weight of 40% glucose solution), and blood glucose was measured at 30, 60, 90, and 120min after gavage, and the area under the blood glucose curve (AUC) of each group of mice was calculated, and the results are shown in FIG. 6.

As shown in fig. 6, the fasting blood glucose was significantly increased in HFD mice compared to the low-fat blank (ND), and the results of the OGTT test: the AUC (area under the curve) of the HFD group was very significantly increased, indicating that high fat diet causes increased blood glucose and impaired glucose tolerance; compared with the HFD group, the fasting blood glucose HL and HH groups are remarkably reduced, and the difference with the ND group mice is not remarkable. OGTT experimental results: AUC (area under the curve) was very significantly reduced in the HL and HH groups compared to the HFD group, and was not significantly different from the ND group. The above proves that: high fat diet can increase fasting blood glucose of mice and damage glucose tolerance, and drinking the composite dark tea can reduce fasting blood glucose and improve body glucose tolerance by normal drinking and drinking concentrated tea.

Liver tissues of each group of mice after feeding for 18 weeks were observed by conventional H & E staining and conventional oil red O staining, and the results are shown in FIGS. 7-9 and Table 1.

As shown in fig. 7 to 9, in the liver pathology sections, compared with the ND group, liver lobular structures were unclear, hepatic sinuses were narrowed or disappeared under pressure, intracellular lipid vacuoles were significantly increased and enlarged, morphology of hepatocytes was changed, and structures were destroyed in the High Fat Diet (HFD) group mice. Compared with HFD, the HE staining of the liver of the HL group of mice can observe the arrangement rule of mouse hepatocyte cords without inflammatory cell infiltration. The results of oil red staining of liver pathology sections were the same as the results of HE staining, and high fat intake resulted in a large amount of lipid deposition in hepatocytes, and high dose of black tea taken together with high fat diet significantly reduced lipid deposition in hepatocytes, whereas the group of black tea (HL) taken at 6mg/ml was very significantly different in reducing lipid deposition in hepatocytes.

As shown in table 1, total cholesterol, triglycerides were significantly elevated in the liver in the HFD group compared to the low fat blank group (ND); compared with the HFD group, the total cholesterol and triglyceride of the HL and HH groups are obviously reduced, and the difference with the ND group is not obvious. Free fatty acids, although not statistically different, showed a marked reduction in the HL and HH groups, with a 50% or greater reduction in the HL group.

TABLE 1 determination of mouse liver tissue lipid deposition-related indices

The above proves that: high fat diet can induce liver deposition, and when the compound dark tea is drunk, the normal drinking of the dark tea and the drinking of a time of concentrated tea can reduce the liver fat deposition, and the effect is better especially when a normal amount (6mg/ml) of dark tea is drunk.

Example 6

Simulating the actual oil level (35% of fat energy supply) and fat intake habit (50% of compound oil fat of lard and soybean oil) of Chinese residents, male C57BL/6J mice of 6 weeks old were used as animal models, and a low-fat blank group (ND, 15% of fat energy supply, free drinking water), a high-fat feed control group (HFD, 35% of fat energy supply, free drinking water), a high-fat + compound ilex normal dose group (KL, 35% of fat energy supply, 6mg/ml of compound ilex prepared in comparative example 2, free drinking tea), a high-fat + compound ilex concentrated tea group (KH, 35% of fat energy supply, 12mg/ml of compound ilex prepared in comparative example 2, free drinking tea) were set for 18 weeks (the ND group and the HFD group in example 6 were the same group as example 5).

The body weights of the mice in each group were measured weekly during feeding, and the final results are shown in FIG. 10.

The body fat percentage, feed intake, perirenal, epididymal, subcutaneous, and mesenteric fat coefficients of the mice in each group after 18 weeks of feeding were measured by the same method as in example 5, and the results are shown in FIG. 11 and FIG. 12.

As shown in fig. 11-12, the low-fat blank group (ND) and HFD group were the same as example 5, and the final body weight of the mice was increased by 25%, which was significantly higher than that of the blank control group (P < 0.01); compared with the HFD group, the body weight of mice in the KL and KH groups is respectively reduced by 11.4 percent and 16 percent, and compared with the embodiment 5, the HL can achieve the high-level (dark tea, KH group) effect of the embodiment 6; the body fat ratios of the KL and KH groups were reduced by 26.7% and 49.8%, respectively, and compared with the HL group of the example 5, the KL was significantly higher than that of the example 6, and the KH effect was not significantly different from that of the example 6. The researches prove that the golden flower poria brick dark tea and the broadleaf holly leaf have synergistic effect, and the effect of the high-dose (2 times) composite broadleaf holly leaf can be achieved by drinking the composite dark tea in normal dose in the embodiment 5.

As shown in fig. 12, compared with HFD group mice, the difference between perirenal and subcutaneous inguinal regions of KL group and KH group mice was not significant, and the fat coefficients of epididymis and mesenteric fat of KH group were significantly and significantly reduced, respectively, and compared with HL and HH group of example 5, the fat coefficients of perirenal, epididymis, subcutaneous and mesenteric fat of HL group mice were less than those of KL group and KH group, indicating that the composite black tea has a better effect of reducing fat of mice than the composite broadleaf holly leaf, and further proving the synergistic effect of the camellia nitidissima brick black tea and the broadleaf holly leaf.

The subcutaneous inguinal adipose tissues and scapular region brown adipose tissues of the respective groups of mice after feeding for 18 weeks were observed by conventional H & E staining using the same method as in example 5, and the results are shown in fig. 13 and 14.

As shown in fig. 13, the KL group had no significant effect on the KH group mouse adipocytes compared to the HFD group; KH fat cells are reduced in size, relatively regular in shape and obvious in effect. Compared with HL and HH groups of the embodiment 5, the fat cells of the HL and HH groups are obviously smaller than the KL group and smaller than the KH group, and the synergistic effect of the golden flower brick dark tea and the broadleaf holly leaf is also proved.

As shown in fig. 14, the mouse adipocytes in KL and KH groups had significantly reduced numbers of lipid droplets, reduced size of adipocytes, relatively regular morphology, and a reduced tendency to fuse into large cells, compared to the HFD group, but the overall effect of HL and HH groups was superior to that of example 6, compared to the HL and HH groups of example 5, demonstrating that the combination of camellia nitidissima and broadleaf holly has a synergistic effect on eliminating lipid deposition in brown adipose tissue.

Fasting blood glucose and glucose tolerance of each group of mice after 18 weeks of feeding were measured by the same method as in example 5, and the results are shown in FIG. 15.

As shown in fig. 15, the fasting plasma glucose KL and KH fractions were significantly and very significantly reduced compared to the HFD group, respectively. The results of the OGTT experiment showed a significant decrease in AUC (area under the curve) for the KL and KH groups compared to the HFD group. The overall effect of HL and HH group was better than that of example 6, and the HL effect was better than that of KH, compared to the HL and HH group of example 5.

The liver tissues of each group of mice after 18 weeks of feeding were observed by conventional H & E staining and oil red O staining in the same manner as in example 5, and the results are shown in FIGS. 16 to 17.

As shown in fig. 16 to 17, the overall effect of liver pathology sections was not significantly different from that of HL and HH groups of example 5.

The combination of the above proves that: the golden flower poria brick dark tea and the broadleaf holly leaf are compounded, the overall effects of reducing blood fat and blood sugar of the normally drunk composite dark tea (6mg/ml) are superior to those of the composite broadleaf holly leaf (12mg/ml), and the compounding of the dark tea and the broadleaf holly leaf has a synergistic effect.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The composite dark tea is characterized by comprising the following components in parts by weight: 50-75 parts of golden flower poria brick dark tea, 9-18 parts of broadleaf holly leaf, 5-15 parts of momordica grosvenori and 0-36 parts of black tea and/or green tea.

2. The composite dark tea as claimed in claim 1, which comprises the following components in parts by weight: 60-65 parts of golden flower poria brick dark tea, 10-15 parts of broadleaf holly leaf, 7.5-10 parts of momordica grosvenori and 10-22.5 parts of black tea and/or green tea.

3. The composite dark tea of claim 1, wherein the black tea comprises any one or more of englery 9, Yunnan black tea, Qimen black tea, and Jinjunmei.

4. The composite dark tea as claimed in claim 1, wherein the green tea comprises any one or more of gold green tea, old husband tippy, dragon well, Biluochun tea.

5. The preparation method of the composite dark tea as claimed in any one of claims 1 to 4, characterized by comprising the following steps: respectively pulverizing the golden flower poria brick dark tea, the broadleaf holly leaf, the momordica grosvenori, the black tea and/or the green tea, and mixing to obtain the composite dark tea.

6. The method according to claim 5, wherein the size of the pulverized particles is 10 to 40 mesh.

7. The preparation method of claim 5, wherein the amount of the compound dark tea is 1.5-3 g/bag.

8. Use of the composite dark tea as claimed in any one of claims 1 to 4 in preparation of food or medicine for improving high fat diet.

9. The use of the composite dark tea as claimed in any one of claims 1 to 4 in the preparation of anti-obesity food or medicament.

10. The use according to any one of claims 8 to 9, wherein the application is performed by: 1-4 bags are taken every day, and each bag is brewed with 250-650 ml of boiling water, kept stand for 2-5 minutes and then drunk freely.