CN114831197B

CN114831197B - Composite black tea and preparation method and application thereof

Info

Publication number: CN114831197B
Application number: CN202210559559.2A
Authority: CN
Inventors: 文驰; 沈程文; 叶孟柯; 程显淯; 王铭涵; 谢芳楠; 徐超富; 刘仲华
Original assignee: Hunan Chuming Tea Industry Co ltd
Current assignee: Hunan Chuming Tea Industry Co ltd
Priority date: 2022-05-23
Filing date: 2022-05-23
Publication date: 2023-08-29
Anticipated expiration: 2042-05-23
Also published as: CN114831197A

Abstract

The invention provides composite black tea and a preparation method and application thereof, and belongs to the technical field of foods and medicines, wherein the composite black tea comprises the following components in parts by weight: 50-75 parts of golden flower Fuzhuan black tea, 9-18 parts of kuding tea, 5-15 parts of fructus momordicae and 0-36 parts of black tea and/or green tea. The compound black tea has small dosage, can obviously reduce the weight, blood sugar, body fat rate, blood fat and liver fat of a high-fat diet mouse, improves insulin sensitivity, and has obvious effects of improving the health level under the high-fat diet condition and preventing obesity.

Description

Composite black tea and preparation method and application thereof

Technical Field

The invention belongs to the technical field of foods and medicines, and particularly relates to composite black tea and a preparation method and application thereof.

Background

It is well known that obesity is the basis of the onset of various chronic diseases such as diabetes mellitus, cardiovascular diseases and the like, one of the most important reasons for obesity is high fat diet, the cooking oil recommended by the world health organization and the Chinese nutrition diet guidelines is 25-30 g/person per day, the fat energy supply ratio is 25% -30%, but the actual cooking oil in China is 42.1 g/person per day, and the diet fat energy supply ratio of urban population and partial village and town rural residents in developed areas is 35%.

The Fuzhuan black tea has the characteristics of pure fragrance, mellow taste, red, yellow and bright soup color and the like because of the growth of Eurotium cristatum (golden flower), but has hunger sensation after drinking; the kuding tea is a tea-like drink taking ilex latifolia with homology of medicine and food as a raw material, has bitter and sweet taste, has the effects of diminishing inflammation, relieving summer-heat, promoting the production of body fluid, quenching thirst, promoting digestion, resolving phlegm and the like, and has the defects of extremely bitter taste, unwilling to drink by most people and the like in life practice. The Fuzhuan black tea and the kuding tea have lipid-lowering effect, but the Fuzhuan black tea and the kuding tea both need to be drunk at high dosage or have obvious effect after effective components are extracted and concentrated, and researches show that people with the weight of 60KG can drink 180-360 g of Fuzhuan black tea or 20-60 g of kuding tea at least daily to achieve the obvious lipid-lowering effect, and the Fuzhuan black tea and the kuding tea are difficult to apply for a long time in life. Fructus Siraitiae Grosvenorii has high nutritive value, and has effects of clearing heat, relieving summer heat, eliminating phlegm, relieving cough, clearing lung-heat, and moistening intestine, and contains mogroside 300 times as sweet as sucrose without generating heat.

At present, under the condition of 35% of fat energy supply of residents in China, the Fuzhuan black tea, the kuding tea, the fructus momordicae and other tea are combined to prepare tea, and related researches on preventing obesity, reducing liver fat deposition and improving blood sugar homeostasis are not reported.

Disclosure of Invention

The invention aims to provide composite black tea and a preparation method and application thereof, and the composite black tea provided by the invention has the advantages that the usage dosage is small, the weight, blood sugar, body fat rate, blood fat and liver fat of a high-fat mouse can be obviously reduced, the insulin sensitivity is improved, and the composite black tea has obvious effects of improving the health level under the high-fat diet condition and preventing obesity.

In order to solve the technical problems, the invention provides the following technical scheme:

the invention provides composite black tea which comprises the following components in parts by weight: 50-75 parts of golden flower Fuzhuan black tea, 9-18 parts of kuding tea, 5-15 parts of fructus momordicae and 0-36 parts of black tea and/or green tea.

Preferably, the composite black tea comprises the following components in parts by weight: 60-65 parts of golden flower Fuzhuan black tea, 10-15 parts of kuding tea, 7.5-10 parts of fructus momordicae and 10-22.5 parts of black tea and/or green tea.

Preferably, the black tea comprises any one or more of Inulae 9, yunnan red tea, qimen black tea and Jinjunmei.

Preferably, the green tea comprises any one or more of golden green tea, ancient hair tips, longjing tea and Biluochun tea.

The invention provides a preparation method of the composite black tea, which comprises the following steps: pulverizing black tea, folum Ilicis, fructus Siraitiae Grosvenorii, black tea and/or green tea respectively, and mixing to obtain compound black tea.

Preferably, the size of the pulverization is 10 to 40 mesh.

Preferably, the split charging amount of the composite black tea is 1.5-3 g/bag.

The invention provides application of the compound black tea in preparing food or medicine for improving high-fat diet.

The invention also provides application of the composite black tea in preparing anti-obesity food or medicine.

Preferably, the application mode is as follows: 1-4 bags per day, each bag is soaked in 250-650 ml of boiling water, and is free to drink after standing for 2-5 minutes.

Compared with the prior art, the invention has the following beneficial effects:

(1) Under the condition of high fat (35% of energy supply of fat), the compound black tea can obviously reduce weight, body fat rate, blood fat and liver fat after long-term drinking, improve insulin sensitivity and reduce blood sugar, and has obvious effects of improving the health level under the condition of high fat diet and preventing obesity.

(2) The composite black tea disclosed by the invention has good taste, is slightly bitter after being sweet after being eaten, and is capable of wetting the throat and quenching thirst, and the defects that the traditional Fuzhuan black tea has hunger sensation, and the taste of kuding tea is extremely bitter are overcome.

(3) The compound black tea disclosed by the invention is small in dosage, and solves the technical problems that the traditional black tea and kuding tea can be used for reducing blood fat, reducing blood sugar and resisting obesity only when being drunk at a high dosage.

(4) The composite black tea provided by the invention is simple and convenient in preparation process, convenient to drink, and suitable for long-term use in places such as families and offices.

Drawings

Figure 1 effect of composite black tea on body weight of high fat diet mice.

Figure 2 effect of composite black tea on final body weight, body fat rate and feed intake of high fat diet mice.

Figure 3 effect of composite black tea on perirenal, epididymal, subcutaneous, mesenteric fat coefficient in high fat diet mice.

FIG. 4 is a graph of H & E staining of subcutaneous adipose tissue of high-fat diet mice with composite black tea (x 200).

FIG. 5 Compound dark tea H & E staining pattern (x 200) of brown adipose tissue of high fat diet mice.

Figure 6 effect of composite black tea on fasting blood glucose and glucose tolerance in high fat diet mice.

FIG. 7H & E staining pattern of liver lipid deposition of composite dark tea on high fat diet mice (x 200).

FIG. 8 is a graph of oil red O staining of liver lipid deposition of high fat diet mice with composite black tea (x 200).

Figure 9 percentage of area stained with oil red O for liver tissue of high fat diet mice with composite black tea.

Note that: # indicates significant difference between HFD and ND groups (P < 0.05), # indicates very significant difference between HFD and ND groups (P < 0.01), x indicates significant difference between drinking composite black tea and HFD groups (P < 0.05), and x indicates very significant difference between drinking composite black tea and HFD groups (P < 0.01).

Figure 10 effect of different concentrations of complex bitter Ding Chashui intervention on mouse body weight.

FIG. 11 Effect of composite Folum Ilicis on body fat Rate of high fat diet mice

Figure 12 effect of composite kuding tea on the perirenal, epididymal, subcutaneous, mesenteric fat coefficient of high fat diet mice.

FIG. 13H & E staining pattern of high fat diet mice subcutaneous fat with composite Folum Ilicis (x 200).

FIG. 14H & E staining pattern of high fat diet mice brown fat with composite Folum Ilicis (x 200).

Figure 15 effect of composite kuding tea on fasting blood glucose and glucose tolerance in mice.

FIG. 16H & E staining pattern of liver lipid deposition of high fat diet mice with composite Folum Ilicis (x 200).

FIG. 17 is a graph of oil red O staining of liver lipid deposition of high fat diet mice with composite Folum Ilicis (x 200).

Note that: compared with the high fat control group, the difference is marked (P < 0.05), and the difference is marked (P < 0.01).

Detailed Description

The invention provides composite black tea which comprises the following components in parts by weight: 50-75 parts of golden flower Fuzhuan black tea, 9-18 parts of kuding tea, 5-15 parts of fructus momordicae and 0-36 parts of black tea and/or green tea; preferably comprises 60 to 65 parts of golden flower Fuzhuan black tea, 10 to 15 parts of kuding tea, 7.5 to 10 parts of fructus momordicae and 10 to 22.5 parts of black tea and/or green tea. In the present invention, all raw material components are commercially available products well known to those skilled in the art unless specified otherwise. According to the invention, the compound black tea can obviously reduce the weight, blood sugar, body fat rate, blood fat and liver fat of a high-fat diet mouse through the synergistic effect of specific amounts of the components on the basis of less dosage than the black tea of the Fuzhuan brick or the Folum Ilicis, and has obvious effects of improving the health level and resisting obesity under the high-fat diet condition.

In the present invention, the black tea preferably includes any one or more of english No. 9, yunnan red, qimen black tea, jinjun eyebrow, and more preferably english No. 9. The green tea preferably comprises one or more of golden green tea, ancient hair tip, longjing tea, and Biluochun tea, and more preferably golden green tea. The source of the compound tea is not particularly limited, and the compound tea can be commercially available products well known to those skilled in the art.

The invention provides a preparation method of the composite black tea, which comprises the following steps: pulverizing the black tea, the kuding tea, the fructus momordicae, the black tea and/or the green tea of the golden flower Fuzhuan brick respectively, mixing and subpackaging to obtain the composite black tea.

In the present invention, the size of the pulverization is preferably 10 to 40 mesh. In the invention, the golden flower dark tea is preferably manufactured into 1 kilogram of finished dark tea per piece by entrusting and customizing, and is preferably crushed to 18-36 meshes, more preferably 24 meshes for standby; the kuding tea is preferably crushed to 18-36 meshes, more preferably 24 meshes for standby; the momordica grosvenori is preferably crushed to 12-24 meshes, more preferably 18 meshes for standby; the black tea and/or green tea is preferably crushed to a mesh size of 18 to 36, more preferably 24.

In the invention, the split charging amount of the compound black tea is preferably 1.5-3 g/bag, more preferably 1.8-2 g/bag.

In the present invention, the application mode is preferably: 1-4 bags per day, each bag is soaked in 250-650 ml of boiling water, and is free to drink after standing for 2-5 minutes. In the invention, the crowd with normal weight (body mass index BMI <24, men < 25) has 1-2 bags per day, each bag is infused with 400-600 ml boiling water, and the bag can be drunk freely after standing for 2-5 minutes; the overweight people (the body mass index BMI is between 24 and 28), 2 to 3 bags are taken every day, each bag is infused with 400 to 500ml of boiling water, and the people can drink freely after standing for 5 minutes; the obese people (body mass index BMI > 28) have 3-4 bags per day, each bag is infused with 300-500 ml boiling water, and the bags are free to drink after standing for 5 minutes.

The technical solutions of the present invention will be clearly and completely described in the following in connection with the embodiments of the present invention. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Example 1

A composite black tea consists of the following components in parts by weight: 50 parts of golden flower Fuzhuan black tea, 9 parts of kuding tea, 5 parts of fructus momordicae and 36 parts of English Red No. 9.

The preparation method of the composite black tea comprises the following steps: pulverizing black tea of Poria brick of flos Lonicerae to 24 mesh, pulverizing Folum Ilicis to 24 mesh, pulverizing fructus Siraitiae Grosvenorii to 18 mesh, and pulverizing Inulae 9 to 24 mesh; mixing the crushed black tea, kuding tea, fructus momordicae and Yinghong No. 9 with the weight portions, and sub-packaging into 3g bags.

Example 2

A composite black tea consists of the following components in parts by weight: 70 parts of golden flower Fuzhuan black tea, 15 parts of kuding tea and 15 parts of fructus momordicae.

The preparation method of the composite black tea comprises the following steps: pulverizing black tea of Poria brick with flos Lonicerae to 40 mesh, pulverizing Folum Ilicis to 10 mesh, and pulverizing fructus Siraitiae Grosvenorii to 12 mesh; mixing the crushed black tea, kuding tea and fructus momordicae according to the weight parts, and sub-packaging into 1.5g per bag.

Example 3

A composite black tea consists of the following components in parts by weight: 60 parts of golden flower Fuzhuan black tea, 10 parts of kuding tea, 7.5 parts of fructus momordicae and 20 parts of golden tea.

The preparation method of the composite black tea comprises the following steps: pulverizing black tea of Poria brick of flos Lonicerae to 30 mesh, pulverizing Folum Ilicis to 24 mesh, pulverizing fructus Siraitiae Grosvenorii to 12 mesh, and pulverizing black tea to 30 mesh; mixing the crushed black tea, kuding tea, fructus momordicae and golden tea according to the weight parts, and sub-packaging into bags with 2g each.

Example 4

A composite black tea consists of the following components in parts by weight: 65 parts of golden flower Fuzhuan black tea, 12.5 parts of kuding tea, 10 parts of fructus momordicae and 22.5 parts of English Red No. 9.

The preparation method of the composite black tea comprises the following steps: pulverizing black tea of Poria brick of flos Lonicerae to 24 mesh, pulverizing Folum Ilicis to 24 mesh, pulverizing fructus Siraitiae Grosvenorii to 18 mesh, and pulverizing Inulae 9 to 24 mesh; mixing the crushed black tea, kuding tea, fructus momordicae and Yinghong No. 9 with the weight portions, and sub-packaging into bags with 2g each.

Comparative example 1

Compared with the example 1, the black tea does not contain the golden flower Fuzhuan black tea, and the rest components are the same.

Compared with the preparation method in the example 1, the preparation method does not contain the treatment of the black tea of the Fuzhuan brick of golden flower, and the other preparation methods are the same.

Comparative example 2

A composite kuding tea consists of the following components in parts by weight: 65 parts of golden green tea, 12.5 parts of kuding tea, 10 parts of fructus momordicae and 22.5 parts of English Red No. 9.

Compared with the preparation method in the example 4, the preparation method is that the black tea of the golden flower Fuzhuan brick is crushed to 24 meshes, the golden green tea is crushed to 24 meshes (or the crushed tea is directly applied and passes through a 24-mesh sieve), and the rest are the same.

Example 5

The actual oil and fat consumption habits (fat energy 35%) and fat intake habits of Chinese residents (50% of compound oil fat of lard and soybean oil respectively) were simulated, a low-fat blank group (ND, fat energy 15% free drinking water), a high-fat feed control group (HFD, fat energy 35% free drinking water), a normal dose group of high-fat+compound black tea (HL, fat energy 35%, 6mg/ml of compound black tea prepared in example 4, free drinking tea) and a high-fat+compound black tea concentrated tea group (HH, fat energy 35%, 12mg/ml of compound black tea prepared in example 4, free drinking tea) were set as animal models, and the animals were fed for 18 weeks.

The body weight of each group of mice was measured weekly during the feeding period and the results are shown in fig. 1.

Body fat rate and feed intake of each group of mice after 18 weeks of feeding were measured, the mice after 18 weeks of feeding were treated for cervical amputation, the kidney, epididymis, subcutaneous, mesenteric fat of the mice were isolated, and fat coefficients were calculated for each fat weight/body weight, respectively, and the results are shown in fig. 2 and 3.

As shown in fig. 1 and 2, on the premise of not affecting the feed intake, the final weight of mice in the HFD group is improved by 25% compared with that of mice in the low-fat blank group (ND), and the mice in the HFD group are extremely higher than that in the blank group (P < 0.01); the mice in HL and HH groups had a 15% and 16% decrease in body weight, respectively, compared to the HFD group, with a very significant difference (P < 0.01); HL and HH group lipid rates were reduced by 44% and 38%, respectively, with significant differences (P < 0.05). The study proves that the compound black tea has the functions of remarkably reducing the weight and remarkably reducing the body fat of obesity caused by high-fat diet, and particularly the body fat rate of a normal-dose tea drinking group is lower.

As shown in fig. 3, the fat coefficient (fat/body weight ratio) of the perirenal, epididymal, subcutaneous inguinal and mesenteric fat was significantly reduced in both HL and HH mice as compared to the HFD mice, indicating that consumption of black tea can reduce abdominal obesity caused by visceral fat deposition.

The subcutaneous inguinal adipose tissue and the brown adipose tissue in the scapular region of the above-mentioned neck-broken sacrificed mice were peeled off, and the subcutaneous inguinal adipose tissue and the brown adipose tissue in the scapular region of each group of mice after 18 weeks of feeding were observed by conventional H & E staining, and the results are shown in fig. 4 and 5.

As shown in fig. 4, effect of composite black tea on subcutaneous inguinal adipocyte morphology and adipocyte size in high fat diet mice: the fat cells of the blank control group (ND) are regular in morphology, clear in cell outline and consistent in size. High Fat Diet (HFD) fat cells are obviously increased, the cell sizes are inconsistent, the morphology is irregular, and the cell intersections are fused, so that the fat diet has a tendency to be fused into large cells. Compared with the HFD group, the HL and HH group mice fat cells have the advantages of reduced fat cell size, relatively regular morphology and slow tendency to fuse into large cells.

As shown in fig. 5, effect of composite black tea on brown adipocyte morphology and adipocyte size of high fat diet mice: the blank (ND) brown adipocytes were regular in morphology and relatively uniform in size. The number of fat drops of brown fat cells in a high fat diet group (HFD) is obviously increased, the size of fat cells is obviously increased, the cell sizes are inconsistent, the morphology is irregular, and the cell intersections are fused, so that the cells tend to be fused into large cells. Compared with the HFD group, the HL and HH group mice fat cells have the advantages that the fat drop number is obviously reduced, the fat cell size is reduced, the morphology is relatively regular, the trend of fusing into large cells is in a slow down state, and the results show that the excessive accumulation of the fat tissues and abnormal enlargement of the fat cells of the mice caused by high-fat diet can be obviously changed by drinking the composite black tea.

Mouse OGTT test: the glucose gastric lavage dose was calculated from the fasting body weight after 10 hours of fasting for each group of mice after 18 weeks of feeding, fasting blood glucose was measured (0 min), glucose gastric lavage was performed at a dose of glucose 3g/kg body weight (i.e., at a dose of 40% glucose solution of 0.0075mL/g body weight), blood glucose was measured 30, 60, 90, 120min after gastric lavage, and the area under the blood glucose curve (AUC) was calculated for each group of mice, and the results are shown in fig. 6.

As shown in fig. 6, mice in the HFD group had significantly elevated fasting blood glucose compared to the low fat blank group (ND), OGTT experimental results: AUC (area under the curve) of HFD group was extremely significantly increased, indicating that high fat diet resulted in increased blood glucose and impaired glucose tolerance; compared with the HFD group, the fasting blood glucose HL and HH groups are extremely obviously reduced, and the difference between the fasting blood glucose HL and HH groups and the ND group mice is not obvious. OGTT test results: AUC (area under the curve) was significantly reduced in HL and HH groups compared to HFD groups, and the difference from ND group was insignificant. The above demonstrates that: the high-fat diet can raise fasting blood sugar and damage glucose tolerance of mice, and the compound dark tea is drunk, and the normal drinking and the drinking of the thick tea can reduce fasting blood sugar and improve organism glucose tolerance.

The liver tissue of each group of mice after 18 weeks of feeding was observed using conventional H & E staining and conventional oil red O staining, and the results are shown in fig. 7 to 9 and table 1.

As shown in fig. 7 to 9, in the liver pathology section, liver lobular structure was not clear in liver tissue of mice in the High Fat Diet (HFD) group, liver sinus was narrowed or disappeared under pressure, intracellular fat vacuoles were significantly increased and enlarged, morphology of hepatocytes was changed, and structure was destroyed, as compared with the ND group. Compared with HFD, the liver HE staining of the HL group mice can observe the arrangement rule of the liver cells of the mice, and the liver HE staining has no inflammatory cell infiltration. The liver pathology section oil red staining result is the same as the HE staining result, intake of high fat leads to a large amount of deposition of lipid in liver cells, intake of high-fat diet and high-dose black tea drinking at the same time can significantly reduce deposition of lipid in liver cells, and 6mg/ml black tea group (HL) drinking has a very significant difference in reducing deposition of lipid in liver cells.

As shown in table 1, total cholesterol, triglycerides were significantly elevated in the livers of the HFD group compared to the low fat blank group (ND); the HL and HH groups showed significantly lower total cholesterol, triglycerides, compared to the HFD group, and no significant differences from the ND group. Free fatty acids, although not statistically different, showed a clear trend in the decrease in HL and HH groups, with a decrease in HL group of more than 50%.

TABLE 1 determination of lipid deposition related indicators in liver tissue of mice

The above demonstrates that: the high-fat diet can induce liver deposition, and the compound black tea is drunk, and the liver fat deposition can be reduced by both normal tea drinking and double-amount concentrated tea drinking, and particularly, the effect of taking normal quantity (6 mg/ml) of black tea is better.

Example 6

The actual oil and fat intake habits (35% of fat energy) and fat intake habits (50% of compound oil fat of lard and soybean oil) of the Chinese residents were simulated, and a low-fat blank group (ND, 15% of fat energy, free drinking water), a high-fat feed control group (HFD, 35% of fat energy, free drinking water), a normal dose group of high-fat and compound kuding tea (KL, 35% of fat energy, 6mg/ml of compound kuding tea prepared in comparative example 2, free drinking tea), a high-fat and compound kuding tea concentrated tea group (KH, 35% of fat energy, 12mg/ml of compound kuding tea prepared in comparative example 2, free drinking tea) were set as animal models, and fed for 18 weeks (ND group, HFD group in example 6 were the same group as in example 5).

The body weight of each group of mice was measured weekly during the feeding period, and the final results are shown in fig. 10.

Body fat percentage, feed intake, and perirenal, epididymal, subcutaneous, and mesenteric fat coefficients of mice were measured for each group of mice after 18 weeks of feeding in the same manner as in example 5, and the results are shown in fig. 11 and 12.

As shown in fig. 11 to 12, the low fat blank (ND) and HFD groups were identical to those of example 5, and the final weight of mice was increased by 25%, which was significantly higher than that of the blank (P < 0.01); KL and KH mice reduced in body weight by 11.4% and 16%, respectively, compared to HFD, HL achieved the high level (strong tea, KH) effect of example 6 compared to example 5; KL and KH group body fat rates were reduced by 26.7% and 49.8%, respectively, significantly higher than KL of example 6 compared to HL group of example 5, with no significant difference in KH effect from example 6. The above researches prove that the black tea of the golden flower Fuzhuan brick and the kuding tea have a synergistic effect, and the effect of high-dose (2 times) composite kuding tea can be achieved by drinking the composite black tea in example 5 at normal dose.

As shown in fig. 12, the difference between the perirenal and subcutaneous inguinal regions of the KL group and KH group mice was not significant, the fat coefficients of epididymis and mesenteric fat of KH group were extremely significant and significantly reduced, respectively, compared with those of HFD group mice, and the HL group mice had smaller perirenal, epididymis, subcutaneous and mesenteric fat coefficients than those of KL group and KH group compared with those of embodiment 5, indicating that the compound black tea achieved a more effect of reducing the fat of mice than that of the compound kuding tea, thereby demonstrating the synergistic effect of the Jinhua Fu brick black tea and kuding tea.

Following the same procedure as in example 5, the mice of each group were observed for subcutaneous inguinal adipose tissue, scapular brown adipose tissue after 18 weeks of feeding by conventional H & E staining, and the results are shown in fig. 13 and 14.

As shown in fig. 13, KL and KH group mouse adipocytes had no significant effect compared to HFD group; KH fat cell size is reduced, morphology is relatively regular, and obvious effect is presented. Compared with the HL and HH groups in the embodiment 5, the sizes of the fat cells in the HL group and the HH group are obviously smaller than those in the KL group and smaller than those in the KH group, and the synergistic effect of the black tea of the Jinhua Fuzhuan brick and the kuding tea is also proved.

As shown in fig. 14, the KL and KH group mice fat cells have significantly reduced fat drop number, reduced fat cell size, relatively regular morphology and a reduced tendency to fuse into large cells compared with the HFD group, but the overall effect of HL and HH group is superior to that of example 6 compared with the HL and HH group of example 5, demonstrating that the golden flower Fuzhuan black tea and kudingcha complex has a synergistic effect on eliminating fat deposition of brown adipose tissue.

The fasting blood glucose and glucose tolerance of each group of mice after 18 weeks of feeding were measured in the same manner as in example 5, and the results are shown in fig. 15.

As shown in fig. 15, fasting blood glucose KL, KH groups were significantly and very significantly reduced, respectively, compared to the HFD group. Results of OGTT experiments showed a significant decrease in AUC (area under the curve) for KL and KH groups compared to HFD groups. The overall effect of the HL and HH groups was superior to example 6, and HL effect was superior to KH, compared to the HL and HH groups of example 5.

In the same manner as in example 5, liver tissues of each group of mice after 18 weeks of feeding were observed using conventional H & E staining and oil red O staining, and the results are shown in FIGS. 16 to 17.

As shown in fig. 16 to 17, the liver pathology sections showed no significant difference in the overall effect compared to the HL and HH groups of example 5.

Comprehensive demonstration: the combined effect of the golden flower Fuzhuan black tea and the kuding tea on reducing blood fat and reducing blood sugar of the combined black tea (6 mg/ml) is superior to that of the combined kuding tea (12 mg/ml), which indicates that the combined black tea and kuding tea has a synergistic effect.

The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims

1. The composite black tea is characterized by comprising the following components in parts by weight: 50-75 parts of golden flower Fuzhuan black tea, 9-18 parts of kuding tea, 5-15 parts of fructus momordicae and 0-36 parts of black tea;

the black tea is Yinghong No. 9.

2. The composite black tea according to claim 1, which comprises the following components in parts by weight: 60-65 parts of golden flower Fuzhuan black tea, 10-15 parts of kuding tea, 7.5-10 parts of fructus momordicae and 10-22.5 parts of black tea;

the black tea is Yinghong No. 9.

3. A composite black tea according to claim 1 or claim 2, wherein the black tea is replaced by green tea;

the green tea is golden green tea.

4. A method for preparing composite black tea according to claim 1 or 2, comprising the steps of: respectively pulverizing the black tea, the kuding tea, the fructus momordicae and the black tea of the golden flower Fuzhuan brick, and mixing to obtain the composite black tea;

the black tea is Yinghong No. 9.

5. A process according to claim 4 wherein the black tea is replaced by green tea;

the green tea is golden green tea.

6. The process according to claim 4, wherein the size of the pulverization is 10 to 40 mesh.

7. The preparation method of claim 4, wherein the split charging amount of the composite black tea is 1.5-3 g/bag.

8. The use of a composite black tea according to any one of claims 1 to 3 in the preparation of a food or medicament for improving a high fat diet.

9. The use of a composite black tea according to any one of claims 1 to 3 in the preparation of an anti-obesity food or drug.

10. The use according to claim 8 or 9, characterized in that the application means are: 1-4 bags per day, each bag is soaked in 250-650 ml of boiling water, and is free to drink after standing for 2-5 minutes.