CN114807639B - Light-driven microbial uranium ore leaching method - Google Patents

Light-driven microbial uranium ore leaching method Download PDF

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CN114807639B
CN114807639B CN202210463251.8A CN202210463251A CN114807639B CN 114807639 B CN114807639 B CN 114807639B CN 202210463251 A CN202210463251 A CN 202210463251A CN 114807639 B CN114807639 B CN 114807639B
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leaching
uranium
uranium ore
medium
ores
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CN114807639A (en
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张道勇
祝鹏烽
张世杰
赵雨薇
谢正国
潘响亮
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Zhejiang University of Technology ZJUT
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Zhejiang University of Technology ZJUT
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    • C—CHEMISTRY; METALLURGY
    • C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
    • C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
    • C22B60/00—Obtaining metals of atomic number 87 or higher, i.e. radioactive metals
    • C22B60/02—Obtaining thorium, uranium, or other actinides
    • C22B60/0204—Obtaining thorium, uranium, or other actinides obtaining uranium
    • C22B60/0217—Obtaining thorium, uranium, or other actinides obtaining uranium by wet processes
    • C22B60/0221—Obtaining thorium, uranium, or other actinides obtaining uranium by wet processes by leaching
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P10/00—Technologies related to metal processing
    • Y02P10/20—Recycling

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  • Life Sciences & Earth Sciences (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental & Geological Engineering (AREA)
  • Geology (AREA)
  • Manufacturing & Machinery (AREA)
  • Materials Engineering (AREA)
  • Mechanical Engineering (AREA)
  • Metallurgy (AREA)
  • Organic Chemistry (AREA)
  • Manufacture And Refinement Of Metals (AREA)

Abstract

The invention discloses a photo-driven microbial uranium ore leaching method. Firstly, blue algae with photosynthesis activity is inoculated into a leaching culture medium, uranium ore particles are added until the uranium concentration is not higher than 30mg/L, then leaching auxiliary agents are added, and supernatant obtained after shaking leaching for 8 days at room temperature and illumination intensity of 2500-3500lux and a rotating speed of 50-200rpm is adopted, thus obtaining uranium leaching liquid. The method can simply and effectively leach uranium ore resources on the premise of low energy consumption and no use of strong acid and alkali reagents, and solves the problems of complicated leaching steps, high cost and more leaching liquor impurities of the existing uranium ore.

Description

Light-driven microbial uranium ore leaching method
Technical Field
The invention relates to a uranium leaching technology, in particular to a method for leaching microbial uranium ores by utilizing light driving, and belongs to the field of mineral leaching.
Background
Uranium is an important strategic element, enriched uranium 235 U is mainly used for nuclear power generation and nuclear and military libraries. Depleted uranium is used as ship ballast and in some cases as aircraft weights, armor plates and armor piercing bullets. The uranium content in the crust of different rock types varies greatly, with an average content of about 2.7mg.kg -1 Making it a richer element than gold or silver reserves.
For many years, the exploitation of uranium ores has led to progressive depletion of high-grade uranium reserves. As the grade of uranium in ores continues to decline, the industry must search for alternative processes to recover uranium from low grade or complex ores that are difficult to process in the prior art. As ore grade decreases, concomitant or continuous leaching of uranium and base metals, rare earth elements, and phosphates is of interest.
Uranium is often distributed in trace amounts in minerals, which makes leaching the most common process for uranium recovery. Many bioleached uranium ores contain a significant amount of pyrite and other iron sulfides, which are oxidized to acidic ferric sulfate. Hydrolysis of ferric iron in the biological iron solution releases protons which, together with biological sulfuric acid, help to meet the acid demand. The alkaline accessory minerals have high acid consumption, and the alkaline accessory minerals need to be neutralized by a sulfuric acid titration method in a presoaking stage, so that a large amount of resources are consumed.
Containing carbonateUranium can in principle be treated by alkaline leaching, involving precipitation of sodium diuranate Na with sodium bicarbonate and sodium carbonate solution 2 U 2 O 7 . Alkaline leaching is generally not suitable for ores with pyrite or other sulphide phases as the main mineral, as they consume large amounts of carbonate. Alkaline bioleaching requires a large amount of resources in the implementation stage, so that the conventional process is extremely costly in treating low-grade and complex ores.
As mentioned above, a photo-driven microbial uranium leaching process would be a very practical method of transuranium leaching.
Disclosure of Invention
The invention provides a light-driven microbial uranium ore leaching method, which realizes a green environment-friendly low-cost uranium ore leaching technology in an illumination environment. The invention has positive significance in uranium ore leaching.
The invention provides a photo-driven microbial uranium ore leaching method, which comprises the following steps: adding uranium ore particles and leaching aids to a leaching medium inoculated with microorganisms; the microorganism is added in an amount of OD based on the volume of the leaching culture medium 680 The uranium ore addition amount is less than or equal to 30mg U/L based on the leaching culture medium area of the total uranium in the final solution, the salinity in the leaching environment is less than or equal to 30g/L, the pH is more than or equal to 7, and the leaching solution is obtained after leaching for 12 days under the conditions that the temperature is room temperature, the illumination intensity is 2500-3500lux and the shaking speed is 50-200 rpm.
The microorganism is a microorganism CO with optical drive 2 Concentrating the blue algae with the mechanism. The microorganism is added in a bacterial precipitate form obtained by fermentation culture, and the fermentation culture method comprises the following steps: inoculating microorganism into growth medium, culturing at room temperature under light/dark for 12h/12h to logarithmic phase to obtain fermentation culture solution, centrifuging at 5500rpm for 20min, removing supernatant, and collecting thallus precipitate.
Further, the leaching medium comprises the following components: 150mg/L NaNO 3 、2mg/L K 2 HPO 4 、7mg/L MgSO 4 ·7H 2 O, 0.5mg/L citric acid, 0.5mg/L ferric ammonium citrate and 2mg/L Na 2 CO 3 The solvent is deionized water, and the pH value is natural.
Further, the uranium ore particles are uranyl phosphate minerals which are sieved by a 100-200 mesh sieve.
Further, the uranium ore addition amount is less than or equal to 30mgU/L based on the leaching culture substrate area based on the total uranium in the final solution.
Further, microorganisms enrich a large amount of inorganic carbon from the atmosphere through photosynthesis, the enriched inorganic carbon can react with uranium ores to dissolve the uranium ores, and leaching of the uranium ores is achieved, wherein the leaching aid is introduced to accelerate dissolution of the uranium ores by forming a complex of calcium-uranium-carbonate groups.
The invention discloses a light-driven microbial uranium ore leaching method which comprises the following steps:
(1) Preparation of microorganisms: inoculating microorganism into growth medium, culturing at 25deg.C under light/dark for 12h/12h to logarithmic phase, centrifuging the culture solution, removing supernatant, and collecting thallus precipitate;
(2) Preparation of a microbial leaching suspension: suspending the bacterial precipitate in leaching culture medium to obtain microbial leaching suspension;
(3) Microbial leaching: adding uranium ore particles according to the addition requirement of the uranium ore into the microbial leaching suspension, adding a leaching aid, and leaching for 8 days under the conditions that the temperature is room temperature, the illumination intensity is 2500-3500lux and the shaking speed is 50-200rpm to obtain uranium leaching solution;
(4) Testing the content of leached uranium: and (3) detecting the leaching efficiency of uranium in the leaching process in the step (3). Specifically, a certain amount of extraction solution is taken, a filter membrane with the diameter of 0.22 mu m is used for filtering to obtain leaching solution, and an inductively coupled plasma emission spectrometer is used for testing the uranium content in the leaching solution, namely the leached uranium content.
(5) Calculation of leaching efficiency:wherein c represents the leached uranium described in step 3, c 0 Representing the total uranium content in the uranium ore particles of step 2。
Detailed Description
The invention will be further described with reference to specific examples, but the scope of the invention is not limited thereto. Taking into account K in BG11 medium 2 HPO 4 、EDTANa 2 The invention designs a leaching culture medium based on a BG11 culture medium, wherein the components and the concentration of the culture medium are as follows: 150mg/L NaNO 3 、2mg/L K 2 HPO 4 、7mg/L MgSO 4 ·7H 2 O, 0.5mg/L citric acid, 0.5mg/L ferric ammonium citrate and 2mg/L Na 2 CO 3 The solvent is deionized water, and the pH value is natural.
The strain used in the embodiment of the invention is the biological CO with optical drive 2 Microorganisms (synechocystis) of the concentration mechanism.
Example 1
(1) Preparation of blue algae: inoculating blue algae into growth medium, culturing at 25deg.C under light/dark for 12 hr/12 hr to logarithmic phase, centrifuging the culture solution at 5500rpm for 20min, removing supernatant, and collecting thallus precipitate;
(2) Preparation of blue algae leaching suspension: the bacterial pellet was suspended in the leaching medium (OD 680 =0.8), to obtain a cyanobacteria leaching suspension;
(3) Leaching blue algae: adding uranium ore particles according to the uranium ore addition requirement (30 mg U/L based on the leaching culture area) into cyanobacteria leaching suspension, and adding 400mg/L CaCl 2 Leaching for 8 days under the conditions of room temperature, 3500lux illumination intensity and 200rpm oscillation to obtain uranium leaching solution;
(4) Testing the content of leached uranium: and (3) carrying out uranium leaching liquid in the step (3), detecting the uranium concentration and calculating leaching efficiency. Specifically, a certain amount of extraction solution is taken, and filtered by a 0.22 mu m filter membrane to obtain leaching solution, the uranium content in the leaching solution is tested by an inductively coupled plasma emission spectrometer, and the formula is adoptedThe leaching efficiency was calculated to be 95.53%.
The result shows that the blue algae can effectively extract uranium ore particles with low content of 30mg U/L.
Comparative example 1
(1) Leaching media containing uranium ore particles: after uranium ore particles with a total uranium content of 30mg U/L are added to the leaching medium, 400mg/L CaCl is added 2 Preparing a leaching culture medium containing uranium ore particles; the leaching medium was not inoculated with blue algae and the other conditions were the same as in example 1.
(2) Testing the content of leached uranium: and (3) detecting the leaching efficiency of uranium in the leaching process in the step (2). Specifically, a certain amount of extraction solution is taken, a filter membrane with the thickness of 0.22 mu m is used for filtering to obtain leaching solution, an inductively coupled plasma emission spectrometer is used for testing the uranium content in the blue algae leaching solution, and the leaching efficiency eta is calculated to be 0%.
The result of comparative example 1 shows that in example 1, blue algae plays a role in effectively leaching uranium.
Example 2
The total uranium content of the uranium ore in example 1 was adjusted to 20mg U/L, caCl 2 The amount added was 200mg/L based on the extraction medium, and the bacterial pellet was suspended in the extraction medium (OD 680 =0.7) light intensity 3000lux, shaking conditions were 100rpm otherwise the same as in example 1, uranium ore leaching was performed, and leaching efficiency was calculated to be 98.89% as a result.
The results of example 2 demonstrate that cyanobacteria is effective in leaching uranium ore particles at low levels of 20mg U/L.
Comparative example 2
The total uranium content of the uranium ore in comparative example 1 was adjusted to 20mg U/L, and blue algae was not inoculated into the leaching medium, and the other conditions were the same as in example 2. Uranium ore leaching was performed and the leaching efficiency was calculated to be 0% at the end.
The comparison example shows that in the embodiment 2, the blue algae has the function of effectively leaching uranium.
Example 3
The total uranium content of the uranium ore in example 1 was adjusted to 10mg U/L, caCl 2 The addition amount is 100mg/L based on the extraction medium, and the bacterial precipitate is suspended in the extractionIn the extraction medium (OD) 680 =0.4), the illumination intensity was 2500lux, the shaking condition was 50rpm, the uranium ore leaching was performed as in example 1, and the leaching efficiency was calculated as 100%.
The results of example 3 demonstrate that cyanobacteria is effective in leaching uranium ore particles at low levels of 10mg U/L.
Comparative example 3
The total uranium content of the uranium ore in comparative example 1 was adjusted to 10mg U/L, and blue algae was not inoculated into the leaching medium, and the other conditions were the same as in example 3. Uranium ore leaching was performed and the leaching efficiency was calculated to be 0% at the end.
The result of comparative example 3 shows that in example 3, blue algae plays a role in effectively leaching uranium.
Example 4
On the basis of the solution of example 1, a uranium ore leaching was performed by adding a salinity of 30g/L of NaCl mass to leaching medium volume, the other way being that of example 1, and finally, leaching efficiency was calculated to be 78.32%.
The results of example 4 demonstrate that cyanobacteria is effective in leaching uranium ores at low levels of 30mg U/L in high salinity environments.
Comparative example 4
On the basis of the solution of the comparative example 1, the uranium ore leaching is carried out by adding the salinity of 30g/L of NaCl mass and leaching culture medium volume ratio, and the other is the comparative example 1, and finally, the leaching efficiency is calculated to be 0%.
The result of comparative example 4 shows that in example 4, blue algae plays a role in effectively leaching uranium.
Example 5
On the basis of the solution of example 2, a uranium ore leaching was performed by adding a salinity of 30g/L of NaCl mass to leaching medium volume, the other way being that of example 2, and finally, leaching efficiency was calculated to be 85.32%.
The results of example 5 show that blue algae are effective in leaching uranium ores at low levels of 20mg U/L in high salinity environments.
Comparative example 5
On the basis of the solution of the comparative example 2, the uranium ore leaching is carried out by adding the salinity of 30g/L of NaCl mass and leaching culture medium volume ratio, and the other is the comparative example 2, and finally, the leaching efficiency is calculated to be 0%.
The result of comparative example 5 shows that in example 5, blue algae plays a role in effectively leaching uranium.
Example 6
On the basis of the solution of example 3, a uranium ore leaching was performed by adding a salinity of 30g/L of NaCl mass to volume of leaching medium, the other way being that of example 3, and finally, leaching efficiency was calculated to be 92.65%.
The results of example 6 demonstrate that cyanobacteria is effective in leaching uranium ores at low levels of 10mg U/L in high salinity environments.
Comparative example 6
On the basis of the solution of the comparative example 3, the uranium ore leaching is carried out by adding the salinity of 30g/L of NaCl mass and leaching culture medium volume ratio, and the other is the comparative example 3, and finally, the leaching efficiency is calculated to be 0%.
The result of comparative example 6 shows that in example 6, blue algae plays a role in effectively leaching uranium.
Comparative example 7
CaCl was added in example 1 2 Otherwise as in example 1, uranium ore leaching was performed, with a final calculated leaching efficiency of 67.7%.
The results of the comparative examples show that the leaching aid has a significant effect of increasing the leaching effect.
The technical scope of the present invention is not limited to the embodiment scheme, the above embodiment is a preferred embodiment, and the professional in the art can make various changes on the embodiment without changing the gist of the embodiment, such as particle size of leached low-grade uranium ore, pH environment, inoculum size of blue algae, illumination intensity, etc. Similar modifications are intended to be within the spirit of the invention.

Claims (3)

1. An optically driven microbial uranium ore leaching method, characterized by comprising the steps of: adding uranium ore particles and leaching aid to a leaching medium inoculated with a microorganism which is a photosynthetically active cyanobacteria, the uranium ore particles containing leaching medium being in suspension prior to the addition of the uranium ore particles containing leaching mediumThe microbial fermentation is carried out, specifically: inoculating microorganism into growth medium, culturing at room temperature under light/dark for 12h/12h to logarithmic phase, centrifuging the culture solution at 5500rpm for 20min, removing supernatant, collecting bacterial precipitate, and adding the bacterial precipitate into suspension of leaching medium containing uranium ore particles; the formula of the leaching culture medium is 150mg/L NaNO 3 、2mg/L K 2 HPO 4 、7mg/L MgSO 4 •7H 2 O, 0.5mg/L citric acid, 0.5mg/L ferric ammonium citrate and 2mg/L Na 2 CO 3 The solvent is deionized water, and the pH value is natural; the leaching auxiliary agent is CaCl added in the leaching process 2 The concentration is 100-400 mg/L; the microorganism is added in an amount of OD based on the volume of the leaching culture medium 680 The uranium ore addition amount is less than or equal to 30mg U/L based on the leaching culture area of the total uranium in the final solution, the salinity in the leaching environment is less than or equal to 30g/L, the pH is more than or equal to 7, and leaching is carried out for 8 days under the shaking conditions of room temperature, illumination intensity of 2500-3500lux and illumination intensity of 50-200rpm, so as to obtain leaching liquor.
2. A method as claimed in claim 1 wherein the uranium ore particles are solid particles containing uranium after passing through a 100-200 mesh screen.
3. A method as claimed in claim 1 wherein the microorganisms enrich the water with inorganic carbon from the atmosphere by photosynthesis, the enriched inorganic carbon being reactive with uranium ores to dissolve the uranium ores to effect leaching of the uranium ores, wherein the leaching aid is introduced to accelerate dissolution of the uranium ores by formation of a calcium-uranium-carbonate complex.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102079580A (en) * 2009-11-27 2011-06-01 南华大学 Plant restoration method for uranium polluted water
CN105714115A (en) * 2016-05-09 2016-06-29 东华理工大学 Carbonate-siliceous-pelitic-type uranium ore bacterium uranium leaching method
CN110016561A (en) * 2019-03-07 2019-07-16 南华大学 The recovery method of uranium in a kind of microalgae cell
CN113936835A (en) * 2021-10-12 2022-01-14 浙江工业大学 Uranium pollution remediation method based on water-blooming cyanobacteria

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102079580A (en) * 2009-11-27 2011-06-01 南华大学 Plant restoration method for uranium polluted water
CN105714115A (en) * 2016-05-09 2016-06-29 东华理工大学 Carbonate-siliceous-pelitic-type uranium ore bacterium uranium leaching method
CN110016561A (en) * 2019-03-07 2019-07-16 南华大学 The recovery method of uranium in a kind of microalgae cell
CN113936835A (en) * 2021-10-12 2022-01-14 浙江工业大学 Uranium pollution remediation method based on water-blooming cyanobacteria

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