CN114807321A - 核酸扩增 - Google Patents

核酸扩增 Download PDF

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Publication number
CN114807321A
CN114807321A CN202210640563.1A CN202210640563A CN114807321A CN 114807321 A CN114807321 A CN 114807321A CN 202210640563 A CN202210640563 A CN 202210640563A CN 114807321 A CN114807321 A CN 114807321A
Authority
CN
China
Prior art keywords
primer
region
nucleotide
nucleic acid
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210640563.1A
Other languages
English (en)
Chinese (zh)
Inventor
K·贝尔霍钦
J·李
P·帕特尔
A·理查德森
S·塔巴克曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Theranos Inc
Original Assignee
Theranos Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2014/030034 external-priority patent/WO2014145296A2/en
Application filed by Theranos Inc filed Critical Theranos Inc
Publication of CN114807321A publication Critical patent/CN114807321A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202210640563.1A 2013-11-22 2014-09-17 核酸扩增 Pending CN114807321A (zh)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US201361908027P 2013-11-22 2013-11-22
US61/908,027 2013-11-22
USPCT/US2014/030034 2014-03-15
PCT/US2014/030034 WO2014145296A2 (en) 2013-03-15 2014-03-15 Nuclei acid amplification
US14/214,850 US9725760B2 (en) 2013-03-15 2014-03-15 Nucleic acid amplification
US14/214,850 2014-03-15
US201462001050P 2014-05-20 2014-05-20
US62/001,050 2014-05-20
CN201480073710.5A CN106255763B (zh) 2013-11-22 2014-09-17 核酸扩增
PCT/US2014/056151 WO2015076919A1 (en) 2013-11-22 2014-09-17 Nucleic Acid Amplification

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201480073710.5A Division CN106255763B (zh) 2013-11-22 2014-09-17 核酸扩增

Publications (1)

Publication Number Publication Date
CN114807321A true CN114807321A (zh) 2022-07-29

Family

ID=53179994

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202210640563.1A Pending CN114807321A (zh) 2013-11-22 2014-09-17 核酸扩增
CN201480073710.5A Active CN106255763B (zh) 2013-11-22 2014-09-17 核酸扩增

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201480073710.5A Active CN106255763B (zh) 2013-11-22 2014-09-17 核酸扩增

Country Status (12)

Country Link
EP (2) EP3957741A1 (cg-RX-API-DMAC7.html)
JP (3) JP2017500020A (cg-RX-API-DMAC7.html)
KR (1) KR20160088316A (cg-RX-API-DMAC7.html)
CN (2) CN114807321A (cg-RX-API-DMAC7.html)
AU (1) AU2014353462A1 (cg-RX-API-DMAC7.html)
BR (1) BR112016011692A2 (cg-RX-API-DMAC7.html)
CA (1) CA2927315A1 (cg-RX-API-DMAC7.html)
ES (1) ES2879823T3 (cg-RX-API-DMAC7.html)
HK (1) HK1232259A1 (cg-RX-API-DMAC7.html)
IL (1) IL245687A0 (cg-RX-API-DMAC7.html)
MX (1) MX370616B (cg-RX-API-DMAC7.html)
WO (1) WO2015076919A1 (cg-RX-API-DMAC7.html)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2904847T3 (es) * 2013-03-15 2022-04-06 Labrador Diagnostics Llc Ligasa de extremos romos termostable y métodos de uso
WO2016044673A1 (en) * 2014-09-17 2016-03-24 Theranos, Inc. Hybrid multi-step nucleic acid amplification
US10822648B1 (en) 2016-07-29 2020-11-03 Labrador Diagnostics Llc Hybrid multi-step nucleic acid amplification
CN110819738A (zh) * 2019-11-20 2020-02-21 拱北海关技术中心 高致病性h7n9禽流感病毒的检测方法及其引物和探针
EP4232600A2 (en) * 2020-10-21 2023-08-30 Illumina, Inc. Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1373812A (zh) * 1999-09-13 2002-10-09 纽亘技术公司 用于多核苷酸序列线性等温扩增的方法及组合物
CN1498274A (zh) * 2000-06-26 2004-05-19 Ŧب������˾ 基于转录的核酸扩增方法和组合物
CN101935697A (zh) * 2010-04-16 2011-01-05 北京原平皓生物技术有限公司 用于核酸序列检测的方法和试剂盒

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5928905A (en) * 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
JP4280519B2 (ja) * 2003-03-07 2009-06-17 アークレイ株式会社 Ｄｎａ増幅法およびそのためのキット
GB0504774D0 (en) * 2005-03-08 2005-04-13 Lingvitae As Method
GB0618046D0 (en) * 2006-09-13 2006-10-25 Lingvitae As Method
WO2008070352A2 (en) * 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
GB0910302D0 (en) * 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
EP2746395B1 (en) * 2011-09-08 2017-11-29 Kabushiki Kaisha DNAFORM Primer set, method for amplifying target nucleic acid sequence using same, and method for detecting mutated nucleic acid using same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1373812A (zh) * 1999-09-13 2002-10-09 纽亘技术公司 用于多核苷酸序列线性等温扩增的方法及组合物
CN102586228A (zh) * 1999-09-13 2012-07-18 纽亘技术公司 用于多核苷酸序列线性等温扩增的方法及组合物
CN1498274A (zh) * 2000-06-26 2004-05-19 Ŧب������˾ 基于转录的核酸扩增方法和组合物
CN101935697A (zh) * 2010-04-16 2011-01-05 北京原平皓生物技术有限公司 用于核酸序列检测的方法和试剂盒

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PRITHIVIRAJ, J.ET AL.: "Rapid detection of microbial DNA by a novel isothermal genome exponential amplification reaction (GEAR) assay", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 17 March 2012 (2012-03-17), pages 1 - 2 *

Also Published As

Publication number Publication date
MX370616B (es) 2019-12-17
EP3071713A4 (en) 2017-08-02
BR112016011692A2 (pt) 2017-09-26
CN106255763B (zh) 2022-06-24
HK1232259A1 (zh) 2018-01-05
JP2019201670A (ja) 2019-11-28
EP3071713B1 (en) 2021-05-12
EP3071713A1 (en) 2016-09-28
EP3957741A1 (en) 2022-02-23
IL245687A0 (en) 2016-06-30
CA2927315A1 (en) 2015-05-28
AU2014353462A1 (en) 2016-05-05
JP2022172384A (ja) 2022-11-15
WO2015076919A1 (en) 2015-05-28
ES2879823T3 (es) 2021-11-23
JP2017500020A (ja) 2017-01-05
MX2016006602A (es) 2016-07-22
CN106255763A (zh) 2016-12-21
KR20160088316A (ko) 2016-07-25

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Application publication date: 20220729