CN114804980A - Urease inhibitor composition and fertilizer composition containing same - Google Patents
Urease inhibitor composition and fertilizer composition containing same Download PDFInfo
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- CN114804980A CN114804980A CN202210742383.4A CN202210742383A CN114804980A CN 114804980 A CN114804980 A CN 114804980A CN 202210742383 A CN202210742383 A CN 202210742383A CN 114804980 A CN114804980 A CN 114804980A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/90—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting the nitrification of ammonium compounds or urea in the soil
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The application discloses a urease inhibitor composition, which is characterized by comprising the following components: a yucca extract; and chemical urease inhibitors. The urease inhibitor composition can reduce the dosage of urease inhibitor, improve the efficacy of urease inhibitor and reduce environmental pollution, and provides a urease inhibitor composition and a fertilizer composition which are suitable for being used on land for a long time without residue.
Description
Technical Field
The invention relates to the field of fertilizer technology synergism, in particular to a urease inhibitor composition, a fertilizer composition containing yucca extract and a preparation method thereof.
Background
Urea is the most widely used nitrogen fertilizer in agricultural production at present, and has obvious effect on the aspects of quality improvement and yield increase of crops. Since urea is applied to the soil, it is first hydrolyzed into NH by urease 4 + Partially with NH 3 The form of which escapes from the soil. Then, NH 4 + Under the action of soil nitrifying bacteria, NO is formed 3 - The whole process only needs 7-10 days. UreaConversion to NH 3 And NO 3 - The faster the rate, the more severe the nitrogen loss. Wherein the urea hydrolysis rate by urease is no-enzyme-catalyzed 10 14 Twice as much as 30-35% of direct utilization of urea by plants, with a annual national loss of nitrogen of about 1.0 x 10 7 And t is about. Moreover, the rapid decomposition of urea not only causes serious waste of nitrogen resources, but also brings about a series of problems of crop nitrite accumulation, water eutrophication and the like. Urease enzymes are widely distributed in nature, for example, biologically and non-biologically. Therefore, how to delay the hydrolysis of urea and improve the utilization rate of urea becomes a problem to be solved urgently in the current agricultural production.
Urease activity is a key factor affecting urea hydrolysis. If the activity of urease in the first step of urea hydrolysis can be controlled, the hydrolysis speed of urea can be delayed, and the loss of nitrogen can be reduced. At present, it is common practice to add a proper amount of urease inhibitor to urea. There are hundreds of urease inhibitors that have been found so far, mainly heavy metals, quinones, polyphenols, phosphoramides, pentachloronitrobenzene, plant extracts, and the like. However, heavy metal urease inhibitors have high toxicity and are easy to pollute the environment, and organic matters have short effective inhibition time, high residue and are difficult to degrade. In addition, the use of these two classes of urease inhibitors tends to increase the cost of input to the farmers and cause secondary damage to the crops and the soil. Therefore, the development of the plant-derived urease inhibitor which is high in efficiency, low in toxicity, free of residues and sufficient in source has important value.
Yucca (Yucca schidigera) belongs to the family of Agavaceae, is a perennial evergreen shrub, mainly grows in dry desert areas in the southwest of the United states and the northern part of Mexico, and is also planted in provinces and cities such as Zhejiang of China, commonly known as pineapple. The yucca extract mainly contains steroid saponin, polysaccharide, polyphenol and other active ingredients, is widely applied in animal husbandry production, can effectively inhibit urease activity of large intestine and small intestine of animals, improve intestinal environment, reduce emission of harmful gases such as ammonia gas in feces, and improve animal house environment. However, the application of the yucca extract in the field of fertilizer urease inhibitors is mentioned at present, and the yucca extract has high development and utilization potential.
Disclosure of Invention
The present application provides a urease inhibitor composition and a fertilizer composition using the same, which is low in cost, high in efficiency, less in environmental pollution, and suitable for long-term use on the land. The present application includes the following embodiments:
Embodiment 2. the urease inhibitor composition according to embodiment 1, wherein the ratio of yucca extract to the chemical urease inhibitor is 10 to 30: 0.1 to 0.3.
Embodiment 4. the urease inhibitor composition according to embodiment 1, wherein the yucca extract is in a powder form; the chemical urease inhibitor is NBPT.
Embodiment 5. the urease inhibitor composition according to embodiment 1, wherein the yucca extract is prepared by the following method:
and (3) extraction: extracting Yucca powder in solvent, filtering to obtain filtrate and obtain extractive liquid;
concentration: concentrating the extract under reduced pressure until the relative density is 1.05-1.25 to obtain an extract;
and (3) drying: and drying the extract to obtain the yucca extract.
decocting: decocting Yucca powder in water, filtering to obtain filtrate, and making into decoction;
primary concentration: concentrating the decoction under reduced pressure until the relative density is 1.05-1.25 to obtain a primary concentrated solution;
and (3) extraction: extracting the primary concentrated solution by using an extraction solvent to prepare an extract liquid;
washing: washing the extract liquor with washing liquid, and removing the washing liquid to obtain washed extract liquor;
and (3) secondary concentration: concentrating the washed extract under reduced pressure to obtain a secondary concentrated solution;
and (3) purification: separating and purifying the secondary concentrated solution by using a macroporous adsorption resin column to obtain an eluent;
and (3) concentrating for the third time: concentrating the eluent under reduced pressure to obtain thick paste;
and (3) drying: and drying the thick paste to obtain the yucca extract.
Embodiment 7. the urease inhibitor composition according to embodiment 6 wherein the extraction solvent in the extraction step is a water saturated n-butanol solution and the wash solution in the washing step is an n-butanol saturated aqueous solution.
Embodiment 8. a fertilizer composition, comprising: nitrogen fertilizer; and a urease inhibitor composition according to any one of embodiments 1-7.
According to the technical scheme, the combination of the chemical urease inhibitor and the yucca extract can reduce the dosage of the urease inhibitor, improve the efficacy of the urease inhibitor and reduce the environmental pollution, and provides the urease inhibitor composition and the fertilizer composition which are suitable for being used on land for a long time without residues.
Drawings
To more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings of the embodiments will be briefly introduced below, and it is apparent that the drawings in the following description relate only to some embodiments of the present disclosure and are not limiting to the present disclosure.
FIG. 1 is a graph showing the dynamic change of the N content of the soil in the urea state under different treatments in example 2;
FIG. 2 shows the dynamic variation of N content in urea state of soil under different treatments in example 3.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present disclosure more apparent, the technical solutions of the embodiments of the present disclosure will be described clearly and completely with reference to the drawings of the embodiments of the present disclosure. It is to be understood that the described embodiments are only a few embodiments of the present disclosure, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the disclosure without any inventive step, are within the scope of protection of the disclosure.
In the present application, each term has a meaning generally understood in the art, unless otherwise indicated or a different meaning can be derived from the context.
The invention discloses a urease inhibitor composition which is characterized by comprising the following components in parts by weight: a yucca extract; and chemical urease inhibitors. The degradation period of the chemical urease inhibitor is long, chemical residues exist and poison human bodies after long-term application, the inhibition effect of the chemical urease inhibitor is easily affected by factors such as moisture, pH, temperature and the like, and the inhibition effect is not mild and stable enough. The yucca extract has good urease inhibiting effect, wide source and mild effect, and the combined application of the chemical urease inhibitor and the yucca extract can greatly reduce the dosage of the chemical urease inhibitor and maintain or even improve the urease inhibiting effect, thereby reducing the residue of the chemical urease inhibitor. In addition, the yucca extract contains rich saponin, and can reduce the effect of continuous cropping of crops, improve the resistance of crops to biotic and abiotic stress, exploit the self-stress resistance of crops and help the quality improvement and synergism of crops besides playing the role of urease inhibitor.
Chemical urease inhibitor in this application refers to urease inhibitors other than plant extracts, which are generally chemically synthesized and have a molecular weight of no more than 500.
In some embodiments, the ratio of yucca extract to the chemical urease inhibitor is 10 to 30: 0.1 to 0.3. Application tests show that if a chemical urease inhibitor is applied alone or a yucca extract is applied alone, the input amount of the chemical urease inhibitor is much less than that of the yucca extract, for example, 1 part by weight of the chemical urease inhibitor has an inhibitory effect equivalent to that of 100 parts by weight of the yucca extract, while the cost of the chemical urease inhibitor is much higher than that of the yucca extract, and if the two are applied alone, the inhibitory effect and the input amount and the input cost cannot be considered, the applicant's inventors have unexpectedly found that when the ratio of the yucca extract to the chemical urease inhibitor is 10 to 30: 0.1 to 0.3, the dosage of the composition can be greatly reduced compared with the dosage of the composition when the composition is used alone, the dosage cost is kept not to be increased or slightly reduced, but the urease inhibiting efficiency is not reduced, and the composition have synergistic effect within the range of the ratio.
The "urease inhibiting efficacy" or "inhibiting efficacy" in the present application refers to the amount of ability to inhibit urease activity, characterized by the amount of urea state content in the soil after a certain period of inhibitor action.
In some embodiments, the ratio of yucca extract to the chemical urease inhibitor is such that: x is more than or equal to 10% and less than or equal to 30%, Y is more than or equal to 0.1% and less than or equal to 0.3%, and X +100Y is more than or equal to 40% and less than or equal to 50%, wherein X is the mass percentage of the yucca extract in the pure nitrogen content of urea, and Y is the mass percentage of the chemical urease inhibitor in the pure nitrogen content of urea. Application tests show that the inhibition efficiency of 1 part by weight of the chemical urease inhibitor is equivalent to that of 100 parts by weight of yucca extract, when the yucca extract and the chemical urease inhibitor are combined for application, the ratio of the yucca extract to the chemical urease inhibitor is within the range satisfying the relationship, compared with the single addition of an equal amount of any one urease inhibitor, the urease inhibition efficiency is remarkably improved, namely the urease inhibition efficiency after the combination of the yucca extract and the chemical urease inhibitor is higher than the simple addition of the inhibition efficiency after the combination of the yucca extract and the chemical urease inhibitor, and the synergistic effect of the two is remarkable in the ratio range. When the method is applied to agricultural planting, the optimal urease inhibiting efficiency can be obtained with lower input amount and input cost.
In some embodiments, the yucca extract is in a powder form; the chemical urease inhibitor is NBPT. N-butyl thiophosphoric triamide (NBPT) is one of the most effective soil urease inhibitors. The yucca extract and NBPT combination can exert respective advantages and obtain better urease inhibiting effect.
In some embodiments, the yucca extract is prepared by a method comprising:
and (3) extraction: extracting Yucca powder in solvent, filtering to obtain filtrate and obtain extractive liquid;
concentration: concentrating the extract under reduced pressure until the relative density is 1.05-1.25 to obtain an extract;
and (3) drying: and drying the extract to obtain the yucca extract.
The preparation method of the yucca extract has the advantages of simple process and convenient operation.
In some embodiments, the yucca extract is prepared by the following method:
decocting: decocting Yucca powder in water, filtering to obtain filtrate, and making into decoction;
primary concentration: concentrating the decoction under reduced pressure until the relative density is 1.05-1.25 to obtain a primary concentrated solution;
and (3) extraction: extracting the primary concentrated solution by using an extraction solvent to prepare an extract liquid;
washing: washing the extract liquor with washing liquid, and removing the washing liquid to obtain washed extract liquor;
and (3) secondary concentration: concentrating the washed extract under reduced pressure to obtain a secondary concentrated solution;
and (3) purification: separating and purifying the secondary concentrated solution by using a macroporous adsorption resin column to obtain an eluent;
and (3) concentrating for the third time: concentrating the eluent under reduced pressure to obtain thick paste;
and (3) drying: and drying the thick paste to obtain the yucca extract.
In some embodiments, the extraction solvent in the extraction step is a water-saturated n-butanol solution and the wash solution in the washing step is an aqueous n-butanol-saturated solution.
Thus prepared
The invention also discloses a fertilizer composition, which comprises: nitrogen fertilizer; and the urease inhibitor composition.
In some embodiments, the nitrogen fertilizer is urea.
In some embodiments, the ratio of yucca extract to the chemical urease inhibitor in the urease inhibitor composition is 10 to 30: 0.1 to 0.3, said urease inhibitor composition being present in said fertilizer composition in an amount of 20wt% to 30wt% of the pure N amount of said nitrogen fertilizer, wherein said chemical urease inhibitor is present in an amount not exceeding 0.3wt% of the pure N amount of said nitrogen fertilizer.
Examples
Example 1
This example discloses the preparation of Yucca extract, leaves of Yucca plant (produced area: Jiangsu Shuyang) were dried at a constant temperature of 50 deg.C, pulverized to powder, and sieved through a 40-mesh sieve to obtain Yucca powder for use. The preparation method of the yucca extract comprises the following steps:
decocting: taking 2kg of sieved yucca powder, adding 12kg of water, decocting for 2 hours, filtering, repeating for 2 times, and mixing filtrates to obtain decoction;
primary concentration: concentrating the decoction under reduced pressure until the relative density is 1.10, and cooling to room temperature to obtain a primary concentrated solution;
and (3) extraction: extracting the primary concentrated solution by using an extraction solvent to prepare an extract liquid; the extraction solvent is a water-saturated n-butanol solution, the volume ratio of the primary concentrated solution to n-butanol is 1:3, until the color of the n-butanol layer becomes light, and n-butanol extraction liquid is combined to prepare extraction liquid;
washing: washing the extract with n-butanol saturated water solution for 2 times, and removing water to obtain washed extract;
and (3) secondary concentration: concentrating the washed extract under reduced pressure, and evaporating organic solvent n-butanol to obtain secondary concentrated solution;
and (3) purification: and (3) adding the secondary concentrated solution to a D101 type macroporous adsorption resin column, eluting with distilled water with the volume 4 times that of the column, and discarding a washing solution. Then eluting with 70% ethanol with 5 times of column volume, and collecting eluate;
and (3) concentrating for the third time: concentrating the eluent under reduced pressure to obtain thick paste;
and (3) drying: and drying the thick paste for 10 hours by using a vacuum drier, crushing and sieving to obtain 34.6g of yucca extract.
Example 2
This example discloses urease inhibiting potency when Yucca Extract (YE) and N-butyl thiophosphoric triamide (NBPT) were administered alone as urease inhibitors.
Test materials: the soil to be tested is 0-20cm plough layer soil of peanut test field of Beijing Arguella International agriculture Limited, and the texture is sandy loam. And removing impurities and residual root systems after the fresh soil is recovered, and sieving the fresh soil for later use by a 2mm sieve after air drying. The test fertilizer urea was an analytically pure reagent, the test yucca plant extract was prepared as in example 1, and the test urease inhibitor N-butyl thiophosphoric triamide (NBPT) was produced by the scientific biotechnology limited of boehanic department.
Test design and method: the experiment was set up with 4 treatments, each repeated 3 times, each treatment in turn being as follows:
treatment 1: blank Control (CK): no material was applied;
and (3) treatment 2: urea treatment (U) alone: applying urea in an amount of 350mg/kg dry soil calculated as pure N;
and (3) treatment: NBPT + urea treatment (0.5% NBPT + U): simultaneously applying urea and urease inhibitor NBPT, wherein the application amount of the urea is 350mg/kg of dry soil according to the amount of pure N, and the addition amount of the urease inhibitor NBPT is 0.5 percent of the amount of the pure N of the urea;
and (4) treatment: yucca extract + urea treatment (50% YE + U): simultaneously applying urea and yucca extract, wherein the amount of urea applied is 350mg/kg dry soil calculated by pure N amount, and the amount of yucca extract added is 50% of the pure N amount of urea.
The bottled soil is cultured by adopting polyethylene, 300g of dry soil is filled in each bottle, and distilled water is added to adjust the water content of the soil to be about 60 percent of the maximum water capacity in the field. Pre-culturing for 1 week, mixing NBPT and Yucca extract with urea, and culturing at 25 deg.C in incubator at constant temperature and humidity under dark condition. Samples (5 g) were taken every 3 days from day 1 to day 15 of culture to determine the content of N in the urea state in the soil. The urea state N is leached by KCl-phenylmercuric acetate and is measured by diacetyl monoxime colorimetry. The results are shown in the following table:
TABLE 1 dynamic variation of N content in urea state (mg/kg) of soil under different treatments
Note: different letters in the same column indicate multiple comparison with significant differences (p < 0.01).
Referring to fig. 1, the results show that there is a certain difference in urea decomposition time among the 4 treatments. During the incubation time, the content of N in the urea state was consistently higher in the treatments with NBPT and Yucca extract than in the urea treatment alone, and the difference between NBPT and Yucca extract was not significant. After 15 days of culture, the content of urea state N in soil is 106.08mg/kg and 116.80mg/kg respectively after the two treatments of adding 0.5% NBPT and 50% YE, which shows that the inhibition efficiency of 100 parts by weight of yucca extract is equivalent to that of 1 part by weight of NBPT, and the yucca extract can be used as a botanical urease inhibitor and has the same effect of inhibiting urea hydrolysis in soil as that of NBPT.
Example 3
This example discloses urease inhibiting efficacy of a combination of Yucca Extract (YE) and N-butyl thiophosphoric triamide (NBPT) as a urease inhibitor composition.
The test materials were the same as in example 2.
Test design and method: the experiment was set up with 7 treatments, each repeated 3 times, each treatment in turn being as follows:
treatment 1: urea treatment (U) is applied alone;
and (3) treatment 2: treating with 0.1% NBPT +10% YE + U;
and (3) treatment: treating with 0.1% NBPT +20% YE + U;
and (4) treatment: treating with 0.1% NBPT +30% YE + U;
and (4) treatment 5: treating with 0.3% NBPT +10% YE + U;
and (6) treatment: treating with 0.3% NBPT +20% YE + U;
and (7) treatment: 0.3% NBPT +30% YE + U treatment.
Wherein the application amount of the urea for each treatment is consistent and is 350mg/kg dry soil according to the amount of pure N; the amounts of NBPT and yucca extract were measured as a percentage of the amount of urea N.
The bottled soil is cultured by adopting polyethylene, 300g of dry soil is filled in each bottle, and distilled water is added to adjust the water content of the soil to be about 60 percent of the maximum water capacity in the field. Pre-culturing for 1 week, mixing urease inhibitor composition with urea and soil, and culturing at 25 deg.C in incubator at constant temperature and humidity in dark condition. Samples (5 g) were taken every 3 days from day 1 to day 27 of culture to determine the content of N in the urea state in the soil. The urea state N is leached by KCl-phenylmercuric acetate and is measured by diacetyl monoxime colorimetry. The results are shown in the following table:
TABLE 2
Dynamic change of N content (mg/kg) of soil urea state under treatment of urease inhibiting compositions with different proportions
Note: different letters in the same column indicate multiple comparison with significant differences (p < 0.01).
Referring to fig. 2, the results show that different ratios of urease inhibiting compositions can retard urea hydrolysis in soil, compared to urea (U) treatment alone. When the ratio of the yucca extract to the chemical urease inhibitor is 10 to 30: 0.1 to 0.3, the urease inhibiting composition can inhibit urease to various degrees and delay urea hydrolysis, and the input amount can be reduced relative to that of YE or NBPT singly applied, but the urease inhibiting effect is not reduced. Wherein the delay time of the treatment with 0.1% NBPT +10% YE + U (treatment 2) and 0.1% NBPT +20% YE + U (treatment 3) is the shortest, and is 3 days; treatment with 0.3% NBPT +30% YE + U (treatment 7) delayed the longest duration, up to 12 days. The urea hydrolysis speed in the soil is gradually slowed down along with the increase of the adding proportion of the yucca extract and the NBPT, and the yucca extract and the NBPT have synergistic effect.
From the data of example 2, it can be seen that the soil urea nitrogen content 15 days after application of either 0.5% NBPT (treatment 3) or 50% YE (treatment 4) alone was slightly greater than 100mg/kg, with essentially the same inhibitory effect.
Comparing the data of example 3, it can be seen that, when the two are applied in combination, the content of soil urea nitrogen is slightly greater than 100mg/kg 15 days after application according to the combination ratio of treatment 4 (0.1% NBPT +30% YE) and treatment 5 (0.3% NBPT +10% YE), which is equal to that of example 2 in which 0.5% NBPT (treatment 3) or 50% YE (treatment 4) is applied alone. As shown in example 2, the inhibitory potency of 100 parts by weight yucca extract was comparable to that of 1 part by weight NBPT, calculated as 0.1% NBPT instead of 10% YE, and treatments 4 and 5 of example 3 were comparable to either 40% YE or 0.4% NBPT, but the combined administration of YE and NBPT resulted in an inhibitory potency that was consistent with 50% YE or 0.5% NBPT as in example 2, i.e., urease inhibiting potency was not reduced by the combined administration of 80% of the urease inhibitor composition administered alone. According to the blending ratio of treatment 6 (0.3% NBPT +20% YE) in example 3, the content of soil urea nitrogen reaches 143.81 mg/kg 15 days after application, is increased by 35% compared with 106.08mg/kg of urea nitrogen obtained by singly applying 0.5% NBPT and acting for 15 days in example 2, and is increased by 24% compared with 116.80mg/kg of urea nitrogen obtained by singly applying 50% YE and acting for 15 days in example 2, and the urease inhibition effect of the combination of the two is higher than that of the simple superposition of the inhibition effects of the two when the two are singly used, and the two have obvious synergistic effect in the blending ratio range.
When a fertilizer composition having urease inhibiting effect is prepared from the urease inhibitor composition disclosed in the present application and urea, and added at the ratio of each treatment in examples 2 and 3, the cost of adding the urease inhibitor per ton of the fertilizer composition is shown in table 3, wherein the cost of NBPT is about 320 yuan/kg and the cost of yucca extract is about 5 yuan/kg.
TABLE 3 cost of different urease inhibitor addition rates
As can be seen from the above table, the addition cost per ton of fertilizer calculated according to the addition ratio of example 2 is 736 yuan/ton for 0.5% NBPT and 1150 yuan/ton for 50% YE, the latter being slightly higher. The calculation of treatments 4 and 5 in example 3, which had the same inhibitory effect as that of example 2, showed that the addition costs of 837.2 yuan/ton (treatment 4) and 671.6 yuan/ton (treatment 5), respectively, were approximately equal to the addition cost of 0.5% NBPT in example 2, and were significantly lower than the addition cost of 50% YE in example 2, indicating that the reasonable combined use of NBPT and yucca extract could reduce the amount of urease inhibitor to obtain the same inhibitory effect as that obtained by the use of the urease inhibitor alone, without increasing the input cost or reducing the input cost.
Generally, the stable urea with the addition of 0.5 percent of NBPT is applied to field crops at a dosage of 50 kg/mu, so that the hydrolysis time of the urea can be prolonged from the original 2-7 days to 14-28 days. Then, the urease inhibitor composition of 0.30% NBPT +10% yucca extract selected in the above examples can achieve the same urease inhibiting effect. By means of the synergistic effect of NBPT and yucca extracts, the optimum urease inhibiting effect can be obtained with lower input amount and input cost when the composition is applied to agricultural planting.
Claims (10)
1. A urease inhibitor composition comprising:
yucca extract; and
chemical urease inhibitors.
2. The urease inhibitor composition according to claim 1 wherein,
the ratio of yucca extract to the chemical urease inhibitor is 10 to 30: 0.1 to 0.3.
3. The urease inhibitor composition according to claim 1 wherein,
the ratio of the yucca extract to the chemical urease inhibitor satisfies the following requirements:
10%≤X≤30%，
y is between 0.1% and 0.3%, and
40%≤X+100Y≤50%，
wherein X is the mass percentage of the yucca extract in the pure nitrogen content of urea,
y is the mass percentage of the chemical urease inhibitor in the pure nitrogen of the urea.
4. The urease inhibitor composition according to claim 1 wherein,
the yucca extract is in powder form;
the chemical urease inhibitor is NBPT.
5. The urease inhibitor composition according to claim 1 wherein the yucca extract is prepared by the process of:
and (3) extraction: extracting Yucca powder in solvent, filtering to obtain filtrate and obtain extractive liquid;
concentration: concentrating the extract under reduced pressure until the relative density is 1.05-1.25 to obtain an extract;
and (3) drying: and drying the extract to obtain the yucca extract.
6. The urease inhibitor composition according to claim 1 wherein the yucca extract is prepared by the process of:
decocting: decocting Yucca powder in water, filtering to obtain filtrate, and making into decoction;
primary concentration: concentrating the decoction under reduced pressure until the relative density is 1.05-1.25 to obtain a primary concentrated solution;
and (3) extraction: extracting the primary concentrated solution by using an extraction solvent to prepare an extract liquid;
washing: washing the extract liquor with washing liquid, and removing the washing liquid to obtain washed extract liquor;
and (3) secondary concentration: concentrating the washed extract under reduced pressure to obtain a secondary concentrated solution;
and (3) purification: separating and purifying the secondary concentrated solution by using a macroporous adsorption resin column to obtain an eluent;
and (3) concentrating for the third time: concentrating the eluent under reduced pressure to obtain thick paste;
and (3) drying: and drying the thick paste to obtain the yucca extract.
7. The urease inhibitor composition according to claim 6 wherein,
the extraction solvent in the extraction step is a water saturated n-butanol solution,
the washing solution in the washing step is an aqueous solution saturated with n-butanol.
8. A fertilizer composition, comprising:
nitrogen fertilizer; and
the urease inhibitor composition according to any one of claims 1-7.
9. The fertilizer composition of claim 8, wherein said nitrogen fertilizer is urea.
10. Fertilizer composition of claim 8,
in the urease inhibitor composition, the ratio of yucca extract to the chemical urease inhibitor is 10 to 30: 0.1 to 0.3 of a fatty acid,
the amount of the urease inhibitor composition is 20wt% to 30wt% of the amount of pure N of the nitrogen fertilizer, wherein the amount of the chemical urease inhibitor is no more than 0.3wt% of the amount of pure N of the nitrogen fertilizer.
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| CN115700239A (en) * | 2022-11-25 | 2023-02-07 | 北京地乐福生物技术开发中心 | Fertilizer synergist and preparation method thereof |
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| CN108837775A (en) * | 2018-09-11 | 2018-11-20 | 安徽万士生物制药有限公司 | One kind extracting steroids saponin and its purification process from yucca |
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| WO2003053486A1 (en) * | 2001-12-20 | 2003-07-03 | Kimberly-Clark Worldwide, Inc. | Absorbent article comprising a yucca extract |
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| CN107162755A (en) * | 2017-06-15 | 2017-09-15 | 盖艳春 | A kind of extract of green grass or young crops containing jelly, moderate-element siliceous fertilizer of yucca extract and preparation method thereof |
| CN108837775A (en) * | 2018-09-11 | 2018-11-20 | 安徽万士生物制药有限公司 | One kind extracting steroids saponin and its purification process from yucca |
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| CN115700239A (en) * | 2022-11-25 | 2023-02-07 | 北京地乐福生物技术开发中心 | Fertilizer synergist and preparation method thereof |
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