CN114804977A - Compound microbial fertilizer special for saline-alkali soil and preparation method thereof - Google Patents
Compound microbial fertilizer special for saline-alkali soil and preparation method thereof Download PDFInfo
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- CN114804977A CN114804977A CN202210377004.6A CN202210377004A CN114804977A CN 114804977 A CN114804977 A CN 114804977A CN 202210377004 A CN202210377004 A CN 202210377004A CN 114804977 A CN114804977 A CN 114804977A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2109/00—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation
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- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
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Abstract
The invention provides a compound microbial fertilizer special for saline-alkali soil and a preparation method thereof, wherein the compound microbial fertilizer comprises a carrier and compound microorganisms; the carrier comprises the following components in percentage by mass: 10-15% of humic acid, 8-12% of attapulgite powder and the balance of organic fertilizer; the complex microorganism comprises paenibacillus polymyxa and bacillus belgii. The invention utilizes the biological organic fertilizer to improve the saline-alkali soil, explores a practical, cheap and suitable method and material for effectively improving the saline-alkali soil, realizes the improvement and the effective utilization of the saline-alkali soil according to the principle of efficiently utilizing waste resources and reducing environmental pollution, enables the saline-alkali soil to become an agricultural available resource, promotes the sustainable development of society, agriculture and economy, and has important promotion significance for the benefit of the resources.
Description
Technical Field
The invention relates to the technical field of soil improvement, in particular to a microbial saline-alkali soil conditioner and a preparation method and application thereof.
Background
Soil is an important natural resource, is a material foundation on which human beings depend to live, and is also the most basic production data of agricultural production. The current global soil saline-alkali degradation problem is serious, soil salinization leads to soil quality reduction, land productivity reduction, crop normal growth limited yield reduction, and ecological environment and social economy healthy sustainable development are seriously influenced. The salinized soil (commonly called saline-alkali soil) of China has the area of about 15 hundred million 667m 2 Wherein the modern salinized soil is about 5.5 hundred million 667m 2 The land area of the land is 4.88 percent of the available land in China, the salinized soil area in the cultivated land is 6.6 percent of the cultivated land area in China, and the land area is mainly distributed in North China, inland northwest, plain at northeast and coastal areas. According to the statistics of the second soil general survey in Shandong province in the 80 th of the 20 th century, the salinized soil area of the whole province is 2100 million 667m 2 The area of the salinized farmland is 1223 ten thousand 667m 2 Respectively account for 12 percent and 10 percent of the total area of the soil and the cultivated land. In recent 40 years, along with the reduction of water, the reduction of precipitation and the increase of water consumption of the yellow river, the underground water level of the area, particularly the alluvial plains area of the yellow river, is obviously reduced, and through the treatment and transformation and artificial fine management of a plurality of years, the salinized soil area is obviously reduced, according to the investigation of the area in 2015, the salinized cultivated land area is completely saved, namely 576.01 ten thousand 667m 2 And the land area accounts for about 5.9 percent of the total land area of the whole province. The yield of the salinized soil is low and unstable, and agricultural production and farmer income increase are seriously influenced.
The saline-alkali soil improvement history is long in research at home and abroad, the measures mainly comprise engineering measures, agricultural measures, chemical measures and biological measures, all measures have certain improvement effect, the unit investment for improvement is high, and whether the improvement technology is accepted or not as a main agricultural production body is mainly considered, whether unit investment, simplicity and convenience are realized or not, the output ratio is mainly considered, and the like. The invention aims to utilize the saline-alkali soil remediation microbial inoculum to enrich and improve microbial communities and functional diversity of rhizosphere micro-domains of saline-alkali crops, promote root growth and development, improve salt tolerance, and achieve the purposes of promoting crop income increase and creating a sustainable agricultural healthy production mode.
Disclosure of Invention
The invention provides a compound microbial fertilizer special for saline-alkali soil and a preparation method thereof. The invention utilizes the biological organic fertilizer to improve the saline-alkali soil, explores a practical, cheap and suitable method and material for effectively improving the saline-alkali soil, realizes the improvement and treatment and the effective utilization of the saline-alkali soil based on the principle of efficiently utilizing waste resources and reducing environmental pollution, enables the saline-alkali soil to become agricultural available resources, promotes the sustainable development of society, agriculture and economy, and has important promotion significance for the benefit of resources
In order to achieve the purpose, the invention adopts the following technical scheme:
the compound microbial fertilizer special for the saline-alkali soil comprises a carrier and compound microorganisms;
the carrier comprises the following components in percentage by mass: 10-15% of humic acid, 8-12% of attapulgite powder and the balance of organic fertilizer;
the complex microorganism comprises paenibacillus polymyxa and bacillus belgii.
The beneficial effects are as follows: the humic acid and attapulgite powder are selected, so that the safety is high, various mineral substances can be effectively released, the soil alkalinity can be effectively improved, the physicochemical property of the saline-alkali soil is improved, the loss of soil moisture is reduced, and the sustainable development of the saline-alkali soil is ensured.
The addition of the paenibacillus polymyxa and the Bacillus belgii can influence the microbial community structure, the number and the diversity of bacteria in the saline-alkali soil, effectively improve the contents of soil organic matters, quick-acting P, quick-acting K and alkaline hydrolysis N, reduce the pH value of the soil, take away free Na + in the saline-alkali soil and fundamentally improve the saline-alkali soil.
Further, a microbial saline-alkali soil conditioner comprises a carrier and compound microorganisms;
the carrier comprises the following components in percentage by mass: 11.8% of attapulgite powder, 10% of humic acid and the balance of organic fertilizer;
the complex microorganism comprises paenibacillus polymyxa and bacillus belgii.
Furthermore, the number of effective viable bacteria of the microbial saline-alkali soil conditioner is not less than 3.0 hundred million/g, the organic matter is not less than 50%, the pH value is 7.5-9.0, and the water content is not less than 30%.
Further, the amount of the Bacillus beiLensis is not less than 1 × 1010/ml, and the bacteria content of the Bacillus beiLensis is not less than 1 × 109/ml;
wherein the addition amount of each microbial inoculum in the soil conditioner is 10-20 ml and 50-100 ml per 1000 g.
Furthermore, the main components of the organic fertilizer are sheep manure and crop straws, and the indexes meet the regulations of NY/T525-2021.
The preparation method of the compound microbial fertilizer special for the alkaline land comprises the following steps:
(1) preparation of the carrier: weighing attapulgite powder, humic acid and organic fertilizer according to the mass percentage, and uniformly mixing for later use;
(2) preparing a compound microorganism: respectively inoculating paenibacillus polymyxa and bacillus belgii to corresponding culture media to carry out stirring fermentation to obtain fermentation liquor, wherein the fermentation temperature is 32-35 ℃, the fermentation time is 48 +/-12 hours, and the stirring speed is 200 r/min; in the fermentation process, stirring is not required all the time, and the stirring can be stopped for 0.5-1 h every 4-6 h. In the fermentation process, the intermittent stirring process is relatively continuous, and the bacteria content can be effectively improved by 1-1.5%. Centrifuging the fermentation liquor, taking the supernatant, and concentrating to a required concentration to obtain a concentrated solution;
(3) mixing the concentrated solution with the carrier.
Further, the centrifugation condition in the step (2) is 4000r/min for 5 min.
Further, in the step (2), the paenibacillus polymyxa fermentation liquor is concentrated to the bacteria content of ≧ 1 × 1010/ml, and the bacillus belgii fermentation liquor is concentrated to the bacteria content of ≧ 1 × 109/ml.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:
(1) the humic acid and the attapulgite powder can effectively improve the alkalinity of the soil, improve the physical and chemical properties of the soil and reduce the loss of soil moisture; the paenibacillus polymyxa and the bacillus belgii can promote the improvement of saline alkali of soil, promote the decomposition of related antibiotic substances and have no inhibiting effect on other bacteria.
(2) The prepared modifier is compounded with the specification of NY884-2012, and the quality guarantee period is not less than 6 months.
(3) The conditioner is applied to the saline-alkali soil to be improved, the pH value of the soil can be effectively reduced by 0.1-0.5 unit by applying the conditioner in the growth period of one crop (about 1 year), the volume weight of the soil is reduced by 10%, the microbial diversity in the soil is improved, and the number of microbial flora in the soil is enriched.
Detailed Description
Example 1
The compound microbial fertilizer special for saline-alkali soil: the carrier comprises the following components in percentage by mass: humic acid 10%, attapulgite powder 8% and organic fertilizer 82%; the complex microorganism includes Paenibacillus polymyxa and Bacillus belgii. The effective viable count of the microbial saline-alkali soil conditioner is not less than 3 multiplied by 109/g, the organic matter is not less than 50%, the pH is 7.5, and the water content is not less than 30%. The content of Bacillus belgii is 1 × 1010/ml, and the content of Bacillus belgii is 1 × 109/ml. The indexes of the organic fertilizer meet the regulations of NY/T525-2021.
The preparation method comprises the following steps:
(1) preparation of the carrier: weighing attapulgite powder, humic acid and organic fertilizer according to the mass percentage, and uniformly mixing for later use;
(2) preparing a compound microorganism: respectively inoculating paenibacillus polymyxa and bacillus belgii to corresponding culture media to carry out stirring fermentation to obtain fermentation liquor, wherein the fermentation temperature is 32 ℃, the fermentation time is 36h, and the stirring speed is 200 r/min;
paenibacillus polymyxa culture medium: 30kg of glucose, 20kg of corn meal and soybean meal, MgSO40.05kg, K2HPO40.5kg and 800kg of water; the paenibacillus polymyxa seed liquid accounts for 1% of the paenibacillus polymyxa culture medium by volume;
b, B-Bacillus beiLeisi culture medium: dipotassium phosphate 0.4kg, magnesium sulfate 0.5kg, sodium chloride 0.5kg, potassium nitrate 1.0kg, soluble starch 10kg and water 600 kg; the volume ratio of the Bacillus belgii seed liquid to the Bacillus belgii culture medium is 2%.
Centrifuging the fermentation liquor, taking the supernatant, and concentrating to a required concentration to obtain a concentrated solution;
centrifuging for 5min under the centrifugal condition of 4000 r/min;
concentrating the fermentation liquid of the Paenibacillus polymyxa to the bacteria content of not less than 1 × 1010/ml, and concentrating the fermentation liquid of the Bacillus belgii to the bacteria content of not less than 1 × 109/ml.
(3) Mixing the concentrated solution with the carrier.
Example 2
The compound microbial fertilizer special for the saline-alkali soil comprises a carrier and compound microorganisms;
the carrier comprises the following components in percentage by mass: humic acid 15%, attapulgite powder 12% and organic fertilizer 73%;
the complex microorganism comprises Paenibacillus polymyxa and Bacillus belgii.
The number of effective viable bacteria of the microbial saline-alkali soil conditioner is not less than 3.0 hundred million/g, the organic matter is not less than 50%, the pH is 9.0, and the water content is not less than 30%.
The content of Bacillus belgii is 1 × 1010/ml, and the content of Bacillus belgii is 1 × 109/ml.
The indexes of the organic fertilizer meet the regulations of NY/T525-2021.
The preparation method comprises the following steps:
(1) preparation of the carrier: weighing attapulgite powder, humic acid and organic fertilizer according to the mass percentage, and uniformly mixing for later use;
(2) preparing a compound microorganism: respectively inoculating paenibacillus polymyxa and bacillus belgii to corresponding culture media to carry out stirring fermentation to obtain fermentation liquor, wherein the fermentation temperature is 35 ℃, the fermentation time is 60 hours, and the stirring speed is 200 r/min; paenibacillus polymyxa culture medium: 30kg of corn meal, 25kg of glucose, MgSO40.08kg, K2HPO41.0kg and 1200kg of water; the paenibacillus polymyxa seed liquid accounts for 1.5 percent of the paenibacillus polymyxa culture medium by volume; b, B-Bacillus beiLeisi culture medium: 1.2kg of potassium nitrate, 0.8kg of dipotassium hydrogen phosphate, 0.9kg of magnesium sulfate, 1.0kg of sodium chloride, 20kg of soluble starch and 1200kg of water; the volume ratio of the Bacillus belgii seed liquid to the Bacillus belgii culture medium is 3%. Centrifuging the fermentation liquor, taking the supernatant, and concentrating to a required concentration to obtain a concentrated solution;
centrifuging for 5min at 4000 r/min; concentrating the fermentation liquid of the Paenibacillus polymyxa to the bacteria content of not less than 1 × 1010/ml, and concentrating the fermentation liquid of the Bacillus belgii to the bacteria content of not less than 1 × 109/ml.
(3) Mixing the concentrated solution with the carrier uniformly (wherein the concentrated solution is added in a manner of air drying for 48h under aseptic condition and then adding according to the proportion).
The effect of the saline-alkali soil remediation microbial inoculum is verified:
in order to verify the salt reduction and yield increase effects of the invention, the inventor successively carries out the verification of the improvement effects of the crops such as wheat in many places such as Shandong Huibai agriculture development Co., Ltd, Lijin county spring and favorite agricultural machinery farmer professional cooperative society, Lijin county salt nest town northern village, Dongyngcity reclamation district industrial crop planting professional cooperative society and the like for two years.
1. Wheat verification results
(1) A verification test is arranged on a wheat test of agriculture development Limited company Bohai Hu Virginian, Huidonghui, 2.26.2020, and a soil sample is collected (before spring top dressing). The soil texture of the test field is medium soil, the salt content of 0-20 cm is 1.85g/kg through test determination, and the salt content of 20-40 cm is 2.28g/kg, and belongs to medium salinized moisture soil. 21.8g/kg of organic matters in 0-20 cm soil, 1.20g/kg of total nitrogen, 115mg/kg of alkaline hydrolysis nitrogen, 44.9mg/kg of available phosphorus, 528mg/kg of quick-acting potassium and 8.24 of pH. The verification results are as follows.
Soil salinity of different varieties of biological product treatment
Note: using SPSS analysis, different letters within the same column indicate significant difference (P <0.05) and the same letters indicate insignificant difference (P > 0.05).
Wheat yield of different varieties of microbial fertilizer
The soil restoration microbial inoculum of different embodiments has obvious effects of reducing salt and increasing yield, the salt reduction amplitude of the restoration microbial inoculum is 18.4-33.89%, the index of 10% specified by a project task book is reached, and the effects of reducing salt and increasing yield of the restoration microbial inoculum are better than those of a commercial organic fertilizer and no added strain auxiliary material.
Claims (8)
1. The compound microbial fertilizer special for the saline-alkali soil is characterized by comprising a carrier and compound microorganisms; the carrier comprises the following components in percentage by mass: 10-15% of humic acid, 8-12% of attapulgite powder and the balance of organic fertilizer; the compound microorganism comprises Paenibacillus polymyxa Yuan-du-017 and Bacillus velezensis Yuan-du-015.
2. The compound microbial fertilizer special for saline-alkali soil as claimed in claim 1, which comprises a carrier and compound microorganisms;
the carrier comprises the following components in percentage by mass: 12% of humic acid, 10.8% of attapulgite powder and the balance of organic fertilizer;
the complex microorganism comprises paenibacillus polymyxa and bacillus belgii.
3. The compound microbial fertilizer special for saline-alkali soil as claimed in any one of claims 1 or 2, wherein the effective viable count of the microbial saline-alkali soil conditioner is not less than 2.0 hundred million/g, the organic matter is not less than 50%, the pH is 7.5-9.0, and the water content is not more than 30%.
4. The salt base as claimed in claim 1 or 2The composite microbial fertilizer special for the land is characterized in that the amount of the Bacillus belgii is not less than 1 x 10 10 /ml；
Wherein the addition amount of each microbial inoculum in the soil conditioner is 10-20 ml and 50-100 ml per 1000 g.
5. The compound microbial fertilizer special for saline-alkali soil as claimed in any one of claims 1 or 2, wherein the indexes of the organic fertilizer meet the regulation of NY/T525-2021.
6. The compound microbial fertilizer special for saline-alkali soil as claimed in any one of claims 1 or 2, and the preparation method thereof, characterized by comprising the following steps:
(1) preparation of the carrier: weighing humic acid, attapulgite powder and organic fertilizer according to mass percent, and uniformly mixing for later use;
(2) preparing a compound microorganism: respectively inoculating paenibacillus polymyxa and bacillus belgii to corresponding culture media to carry out stirring fermentation to obtain fermentation liquor, wherein the fermentation temperature is 32-35 ℃, the fermentation time is 48 +/-12 hours, and the stirring speed is 200 r/min; centrifuging the fermentation liquor, taking the supernatant, and concentrating to a required concentration to obtain a concentrated solution;
(3) mixing the concentrated solution with the carrier.
7. The method according to claim 6, wherein the centrifugation conditions in step 6.(2) are 4000r/min for 5 min.
8. The method according to claim 6, wherein in step 6.(2), the fermentation broth of Paenibacillus polymyxa is concentrated to a cell content of 1X 1010/ml or more, and the fermentation broth of Bacillus belgii is concentrated to a cell content of 1X 10 or more 9 /ml。
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130288341A1 (en) * | 2010-10-04 | 2013-10-31 | Sitaram Prasad Paikray | Microbial reclamation of saline and sodic soil |
| CN106367074A (en) * | 2016-08-25 | 2017-02-01 | 北京康缘益生生物科技有限公司 | Compound inoculant for improvement of saline-alkali soil and preparation and application of same |
| CN109055267A (en) * | 2018-08-21 | 2018-12-21 | 山东佐田氏生物科技有限公司 | One plant of saline-alkali tolerant Paenibacillus polymyxa and its application |
| CN111235069A (en) * | 2020-03-17 | 2020-06-05 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Microbial agent M2 for preventing and treating stem basal rot of corn and preparation method thereof |
| CN112500229A (en) * | 2020-12-21 | 2021-03-16 | 东北农业大学 | Microbial water flush fertilizer for improving saline-alkali soil and application thereof |
| CN112500865A (en) * | 2020-12-17 | 2021-03-16 | 江苏植丰生物科技有限公司 | Alkaline soil remediation microbial inoculum and preparation method thereof |
| CN113637483A (en) * | 2021-07-28 | 2021-11-12 | 微生物肥料技术研究推广中心 | Microbial saline-alkali soil conditioner and preparation method and application thereof |
-
2022
- 2022-04-09 CN CN202210377004.6A patent/CN114804977A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130288341A1 (en) * | 2010-10-04 | 2013-10-31 | Sitaram Prasad Paikray | Microbial reclamation of saline and sodic soil |
| CN106367074A (en) * | 2016-08-25 | 2017-02-01 | 北京康缘益生生物科技有限公司 | Compound inoculant for improvement of saline-alkali soil and preparation and application of same |
| CN109055267A (en) * | 2018-08-21 | 2018-12-21 | 山东佐田氏生物科技有限公司 | One plant of saline-alkali tolerant Paenibacillus polymyxa and its application |
| CN111235069A (en) * | 2020-03-17 | 2020-06-05 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Microbial agent M2 for preventing and treating stem basal rot of corn and preparation method thereof |
| CN112500865A (en) * | 2020-12-17 | 2021-03-16 | 江苏植丰生物科技有限公司 | Alkaline soil remediation microbial inoculum and preparation method thereof |
| CN112500229A (en) * | 2020-12-21 | 2021-03-16 | 东北农业大学 | Microbial water flush fertilizer for improving saline-alkali soil and application thereof |
| CN113637483A (en) * | 2021-07-28 | 2021-11-12 | 微生物肥料技术研究推广中心 | Microbial saline-alkali soil conditioner and preparation method and application thereof |
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Application publication date: 20220729 |