CN114800746B - Method for preparing fiberboard by using coriolus versicolor - Google Patents
Method for preparing fiberboard by using coriolus versicolor Download PDFInfo
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- CN114800746B CN114800746B CN202210521180.2A CN202210521180A CN114800746B CN 114800746 B CN114800746 B CN 114800746B CN 202210521180 A CN202210521180 A CN 202210521180A CN 114800746 B CN114800746 B CN 114800746B
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- coriolus versicolor
- fiberboard
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- wood processing
- solid fermentation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27L—REMOVING BARK OR VESTIGES OF BRANCHES; SPLITTING WOOD; MANUFACTURE OF VENEER, WOODEN STICKS, WOOD SHAVINGS, WOOD FIBRES OR WOOD POWDER
- B27L11/00—Manufacture of wood shavings, chips, powder, or the like; Tools therefor
- B27L11/08—Manufacture of wood shavings, chips, powder, or the like; Tools therefor of wood fibres, e.g. produced by tearing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27N—MANUFACTURE BY DRY PROCESSES OF ARTICLES, WITH OR WITHOUT ORGANIC BINDING AGENTS, MADE FROM PARTICLES OR FIBRES CONSISTING OF WOOD OR OTHER LIGNOCELLULOSIC OR LIKE ORGANIC MATERIAL
- B27N3/00—Manufacture of substantially flat articles, e.g. boards, from particles or fibres
- B27N3/04—Manufacture of substantially flat articles, e.g. boards, from particles or fibres from fibres
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
Abstract
The invention discloses a method for preparing a fiberboard by utilizing coriolus versicolor, which comprises the following steps: medium configuration: mixing soybean meal accounting for 5-30% of the weight of the wood processing residues, and then adding a proper amount of tap water to uniformly mix to prepare a culture medium; and (3) solid fermentation: inoculating coriolus versicolor seed liquid into the culture medium according to the mass ratio of 5-10%, and fermenting at 28-30deg.C for 10-20 days to obtain solid fermentation product; preparing a fiber board: the solid fermentation is pressed into a fiberboard. The invention mainly carries out biological strengthening treatment on the wood processing residues added with the bean pulp powder through the coriolus versicolor, thereby realizing the generation of porous structures of the wood processing residues, the generation of lignocellulose and active free radical ions, the purification of bean pulp glue, the secretion of hydrophobin and the like, and further preparing the fiber board with high strength and good water resistance without adding any chemical adhesive and waterproof agent, and being environment-friendly and pollution-free.
Description
Technical Field
The invention relates to the field of fermentation engineering and forestry engineering, in particular to a method for preparing a fiberboard by using coriolus versicolor.
Background
The fiber board is an artificial board made of wood fiber or other plant fiber as raw material and adhesive applied, and is an effective way for comprehensive utilization of wood resources. However, the existing fiberboard is deformed by warping due to expansion force difference after moisture absorption, and has poor water resistance, and a waterproofing agent with high proportion needs to be additionally added. Through incomplete statistics, more than 100 kg of adhesive and 30-50 kg of waterproofing agent are required to be added to produce a fiberboard of 1 cubic meter. The adhesive causes serious environmental pollution in the production process of the fiber board, and endangers the health of production workers; toxic and harmful gases such as formaldehyde remain in the fiberboard prepared by the adhesive, and the prepared fiberboard meets the national standards such as E1 and E2 for releasing the formaldehyde of the fiberboard, but the fiberboard can harm human health for a long time due to long release period. In addition, the addition of the adhesive and the waterproof agent makes the production cost of the fiberboard high, and is one of the main factors of low profit margin of a fiberboard enterprise.
In order to solve the problem of aldehyde-based adhesives, researchers have developed formaldehyde-free adhesives such as melamine, soy protein adhesives, etc. in various ways, these adhesives still have various problems during use. The inventor conducts biodegradation and modification research for a long time, and discovers that the wood fiber raw material is treated by using specific white rot fungi to generate the function similar to an adhesive, so that the wood fiber raw material is prepared into a glue-free fiberboard, and applies for series of patents (201710833075.1, 201710832437.5, 201710832440.7, 201710833090.6 and 201710833104.4), but the problems of insufficient strength, slightly poor water resistance and the like of the fiberboard still exist.
The soybean meal powder is a byproduct of soybean oil processing, contains high content of soybean fibers and crude protein, and also generates micromolecular protein in the process of pressing soybean oil. Because the soybean fiber is mainly hemicellulose, both hemicellulose and micromolecular protein are unfavorable for the production of fiber boards, and the soybean crude protein is difficult to disperse, the soybean meal powder is directly used as an adhesive, and the application problem exists.
Hydrophobins, which are generally composed of about 100 amino acid residues, contain a large number of hydrophobic amino acids and 4 disulfide bonds, are the most surface-active protein species known to date and are considered to be safe for food and medical use. The assembly of hydrophobins on the surface of medical implants can improve the biocompatibility of the implants or can prevent microbial cells from adhering to the surface of a catheter, thereby achieving the aim of protecting the implant surface; in the aspect of fruit and vegetable fresh-keeping, the hydrophobin can form a layer of anti-corrosion fresh-keeping film on the surfaces of the fruits and vegetables; hydrophobin can also be used as a component of a cleaning product to form a stable foam; can be used for promoting the degradation of pollutants in soil and being applied to the process of recovering petroleum after petroleum leakage. At present, no report has been found on the use of hydrophobins in fiberboard to increase the water resistance of the fiberboard.
A large medicinal fungus of Coriolus versicolor can secrete various lignocellulose enzymes, protease, etc., and can grow on sapropel. Whether the bean pulp powder is used as an adhesive or not can be solved by utilizing the growth of the coriolus versicolor in the wood processing residues containing the bean pulp powder, and the problem that the strength and the water resistance of the prepared fiber board are insufficient can also be solved, and no related report is yet seen at present.
Disclosure of Invention
The invention aims to: aiming at the problems in the prior art, the invention provides a reinforcing technology for preparing a fiberboard by using coriolus versicolor, and the reinforcing technology is applied to the preparation of the fiberboard, so that the problems in the background technology can be effectively solved.
The technical scheme is as follows: the invention provides a method for preparing a fiberboard by utilizing coriolus versicolor, which comprises the following steps: medium configuration: mixing soybean meal accounting for 5-30% of the weight of the wood processing residues, and then adding proper tap water to uniformly mix to prepare a culture medium; and (3) solid fermentation: inoculating coriolus versicolor seed liquid into the culture medium according to the proportion of 5-10% after sterilization, and fermenting at 28-30deg.C for 10-20 days to obtain solid fermentation product; preparing a fiber board: pressing the solid fermentation product into a fiberboard.
Preferably, inoculating a coriolus versicolor inclined plane strain to an inclined plane of a potato culture medium for activation, culturing until mycelia grow to be full of the inclined plane, inoculating a mycelia inclined plane block into the potato liquid culture medium for culturing for 4-6 d at the temperature of 25-30 ℃ for 130-180 r/min, and then gradually amplifying to a proper volume according to the inoculation volume percentage of 8-12%, wherein the fermentation condition is 180-220 r/min, and the ventilation rate is 1: and (3) culturing for 3-5 days at the temperature of 25-30 ℃ and 0.6-0.9 to obtain the coriolus versicolor seed liquid.
Further, before solid fermentation, sterilizing the culture medium, and then inoculating the coriolus versicolor seed liquid into the culture medium.
Preferably, in the step of preparing the culture medium, a proper amount of tap water is added, and then the water content of the mixture is adjusted to 50-70%.
Preferably, in the solid fermentation step, the humidity of the system is kept at 85-95%.
Preferably, the wood processing residues comprise wood shavings, sawdust and/or shoots. The wood species are not limited.
Preferably, the wood processing residues are subjected to a pulverizing treatment before use. The pulverizing method is not limited.
Preferably, the soybean meal is also subjected to a pulverization treatment before use, so that it can be uniformly mixed with the wood processing residues.
Preferably, both the wood processing remainder and the bean poise powder are crushed to above 40 mesh.
Preferably, in the fiberboard preparation step: the solid fermentation is pressed into fiberboard using conventional wet processes.
The beneficial effects are that:
in the preparation step of the culture medium, bean pulp powder is added into wood processing residues, dietary fibers and small molecular proteins in the bean pulp powder promote the growth of coriolus versicolor in solid fermentation, and the secretion of hydrophobin and lignocellulose is enhanced; the utilization of non-protein components in the soybean meal powder realizes the biological purification of soybean meal protein, and the biological modification of coriolus versicolor strengthens the performance of the soybean meal glue.
In the solid fermentation step, the coriolus versicolor can secrete high-content lignocellulose, and the enzyme can further carry out enzymatic modification on wood processing residues to generate active sites with porous structures and the like which are beneficial to gluing reaction; the coriolus versicolor rapidly utilizes dietary fibers and small molecular proteins in the soybean meal powder, secretes corresponding protease, and further modifies residual macromolecular soybean meal proteins to generate active groups beneficial to an adhesive reaction; coriolus versicolor undergoes penetration growth in wood processing residues and secretes large amounts of hydrophobins.
In the preparation step of the fiber board, when the fiber board is hot-pressed by a wet method, a large amount of active free radical ions are generated by the lignocellulose, and a strong gluing reaction is generated by combining a porous structure of wood processing organisms, multiple active groups of bean pulp glue and the like, so that no chemical gluing agent is required to be added. In addition, the hydrophobic groups of the hydrophobin can make the fiber board hydrophobic from inside to outside, so that the water absorption expansion rate of the fiber board is reduced.
In contrast to the prior art, the method has the advantages that,
1. the fiber board prepared by the invention has high strength and strong water resistance. The biological treatment effect of the coriolus versicolor is enhanced by utilizing the bean pulp powder, so that the generation of a porous structure of wood processing residues, the generation of lignocellulose and active free radical ions, the purification of bean pulp glue, the secretion of hydrophobin and the like are realized, and the adhesive effect is enhanced and the hydrophobicity is higher when the board is manufactured by hot pressing;
2. the invention is green and environment-friendly, and has no pollution. The fiber board is produced without any aldehyde-based adhesive, and the wood processing residues treated by the coriolus versicolor are all used for producing the fiber board, so that no waste is generated.
In conclusion, the invention uses the coriolus versicolor to carry out biological strengthening treatment in the wood processing residues added with the bean pulp powder, and is totally used for producing the fiber board, no chemical adhesive or waterproof agent is added in the process, and the prepared fiber board has high strength, good water resistance, environmental protection and no pollution.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Preparation of coriolus versicolor seed liquid
Inoculating the coriolus versicolor inclined plane strain preserved in a refrigerator onto a potato culture medium inclined plane for activation, culturing until mycelia grow to be full of the inclined plane, inoculating 3 mycelia inclined plane blocks into 100mL potato liquid culture medium for culturing, culturing for 5d at 28 ℃ at 150r/min, and gradually amplifying to a proper volume according to 10% of inoculation volume percentage, wherein the fermentation condition is 200r/min, and the ventilation rate is 1: culturing at 28deg.C for 4d at 0.8 to obtain coriolus versicolor seed liquid.
Example 1 preparation of fiberboard Using Cooperation of Sophora Alternifolia powder and Coriolus versicolor
Pulverizing poplar wood shavings to 60 meshes by using a pulverizer, pulverizing soybean meal to 60 meshes, adding the soybean meal powder into the poplar wood shavings powder according to the mass ratio of 20%, and adding tap water with a certain volume to control the water content to be about 60%. And (3) inoculating coriolus versicolor seed liquid according to the mass ratio of 10% after sterilization, controlling the temperature to 30 ℃ and the humidity to 90%, and performing solid fermentation for 15 days to obtain a solid fermentation product. The solid ferment is used for hot pressing fiber board, and conventional wet pressing method is adopted. The bending strength and the water absorption expansion rate of the prepared fiber board reach 28.9MPa and 15.1 percent respectively.
Example 2 preparation of fiberboard Using Cooperation of Sophora Alternifolia powder and Coriolus versicolor
Cutting poplar branches, crushing the poplar branches to 40 meshes, crushing soybean meal to 60 meshes, adding the crushed soybean meal into the poplar branch powder according to a weight ratio of 5%, and adding tap water with a certain volume to control the water content of the poplar branch powder to be about 60%. And (3) inoculating coriolus versicolor seed liquid according to the mass ratio of 5% after sterilization, controlling the temperature to 28 ℃ and the humidity to 90%, and performing solid fermentation for 20 days to obtain a solid fermentation product. The solid ferment is used for hot pressing fiber board, and conventional wet pressing method is adopted. The bending strength and the water absorption expansion rate of the prepared fiber board respectively reach 26.3MPa and 17.4 percent.
EXAMPLE 3 preparation of fiberboard Using Cooperation of Sophora Alternifolia powder and Coriolus versicolor
Pulverizing pine sawdust to 60 meshes, pulverizing soybean meal to 60 meshes, adding into pine sawdust powder according to a weight ratio of 30%, and adding tap water with a certain volume to control the water content to be about 60%. Inoculating coriolus versicolor seed liquid according to mass ratio of 8% after sterilization, controlling temperature at 29 deg.C and humidity at 90%, and performing solid fermentation for 10 days to obtain solid fermentation product. The solid ferment is used for hot pressing fiber board, and conventional wet pressing method is adopted. The bending strength and the water absorption expansion rate of the prepared fiber board reach 29.5MPa and 16.7 percent respectively.
Comparative example 1 preparation of fiberboard directly using poplar wood shavings
Pulverizing poplar wood shavings to 60 mesh with pulverizer, adding tap water with certain volume to control water content to 60%, and hot-pressing to obtain fiber board by conventional wet pressing method. The bending strength and the water-absorbing expansion rate of the prepared fiber board are respectively 12.47MPa and 35.3 percent.
Comparative example 2 preparation of fiberboard Using only coriolus versicolor
The poplar wood shavings are crushed to 60 meshes by a crusher, and tap water with a certain volume is added to control the water content of the poplar shavings to be about 60 percent. And (3) inoculating coriolus versicolor seed liquid according to the proportion of 10% after sterilization, controlling the temperature to 30 ℃ and the humidity to 90%, and performing solid fermentation for 15 days to obtain a solid fermentation product. The solid ferment is used for hot pressing fiber board, and conventional wet pressing method is adopted. The bending strength and the water absorption expansion rate of the prepared fiber board respectively reach 21.92MPa and 27.1 percent.
The foregoing embodiments are merely illustrative of the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the present invention and to implement the same, not to limit the scope of the present invention. All equivalent changes or modifications made according to the spirit of the present invention should be included in the scope of the present invention.
Claims (10)
1. A method for preparing a fiberboard by using coriolus versicolor is characterized in that: the method comprises the following steps:
medium configuration: mixing soybean meal accounting for 5-30% of the weight of the wood processing residues, and then adding a proper amount of tap water to uniformly mix to prepare a culture medium;
and (3) solid fermentation: inoculating coriolus versicolor seed liquid into the culture medium according to the mass ratio of 5-10%, and fermenting at 28-30deg.C for 10-20 days to obtain solid fermentation product; in the solid fermentation process, the coriolus versicolor secretes lignocellulose to carry out enzymatic modification on wood processing residues to generate active sites which are beneficial to gluing reaction; the coriolus versicolor utilizes dietary fibers and small molecular proteins in the bean pulp powder, and secretes corresponding protease to further modify residual macromolecular bean pulp proteins to generate active groups beneficial to an adhesive reaction; the coriolus versicolor also performs penetration growth in wood processing residues and secretes a large amount of hydrophobins;
preparing a fiber board: pressing the solid fermentation product into a fiberboard through wet hot pressing; when the fiber board is hot-pressed by a wet method, the lignocellulose enzyme generates a large amount of active free radical ions, and combines the porous structure of wood processing residues and the multi-active groups of proteins in the soybean meal powder to generate strong gluing reaction.
2. The method for preparing a fiberboard by using coriolus versicolor according to claim 1, wherein: the preparation method of the coriolus versicolor seed liquid comprises the following steps:
inoculating a coriolus versicolor inclined plane strain onto a potato culture medium inclined plane for activation, culturing until hypha grows to be full of the inclined plane, inoculating a hypha inclined plane block into a potato liquid culture medium for culturing for 4-6 d at the temperature of 25-30 ℃ at the speed of 130-180 r/min, and amplifying the strain to a proper volume step by step according to the inoculation volume percentage of 8-12%, wherein the fermentation condition is 180-220 r/min, and the ventilation rate is 1: and (3) culturing for 3-5 days at the temperature of 25-30 ℃ and 0.6-0.9 to obtain the coriolus versicolor seed liquid.
3. The method for preparing a fiberboard by using coriolus versicolor according to claim 1, wherein: before solid fermentation, sterilizing the culture medium, and then inoculating the coriolus versicolor seed liquid into the culture medium.
4. The method for preparing a fiberboard by using coriolus versicolor according to claim 1, wherein: in the step of preparing the culture medium, a proper amount of tap water is added, and then the water content of the mixture is 50-70%.
5. The method for preparing a fiberboard by using coriolus versicolor according to claim 1, wherein: in the solid fermentation step, the humidity of the system is kept at 85-95% in the fermentation process.
6. The method for preparing a fiberboard by using coriolus versicolor according to claim 1, wherein: the wood processing residues include wood shavings, sawdust and/or shoots.
7. The method for preparing a fiberboard by using coriolus versicolor according to claim 1, wherein: the wood processing residues are subjected to a pulverizing treatment before use.
8. The method for preparing a fiberboard using coriolus versicolor according to claim 7, wherein: the bean pulp powder is also crushed before use, so that the bean pulp powder can be uniformly mixed with the wood processing residues.
9. The method for preparing a fiberboard using coriolus versicolor according to claim 8, wherein: and crushing the wood processing residues and the bean pulp powder to above 40 meshes.
10. The method for producing a fiberboard using coriolus versicolor according to any one of claims 1 to 9, characterized in that: in the fiberboard preparation step: the solid fermentation is pressed into fiberboard using conventional wet processes.
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| DE102005014843A1 (en) * | 2005-03-30 | 2006-10-19 | Basf Ag | Use of hydrophobin for treating surfaces such as hardened mineral building materials, natural stones, artificial stones or ceramics |
| CN102650108B (en) * | 2012-04-19 | 2014-06-04 | 华中科技大学 | Method for producing fiberboards by lignocelluloses as raw materials |
| CN106634818A (en) * | 2016-12-27 | 2017-05-10 | 常州大学 | Preparation method of biological protein-based water-tolerant wood adhesive |
| CN109849147A (en) * | 2018-11-09 | 2019-06-07 | 中国林业科学研究院林产化学工业研究所 | One kind is based on tannin based cross-linker modified bean pulp glue medium density fibre board (MDF) and preparation method thereof |
| CN109624017A (en) * | 2018-12-13 | 2019-04-16 | 北京服装学院 | A kind of fiberboard and preparation method thereof based on fungi |
| CN111732928A (en) * | 2020-07-10 | 2020-10-02 | 宁波中科朝露新材料有限公司 | Method for preparing soybean-based adhesive by adopting soybean meal for feed and application of soybean-based adhesive |
| CN111793464B (en) * | 2020-07-14 | 2021-12-07 | 南宁雄晋生物科技有限公司 | Method for producing soybean-based protein glue by enzyme method modified soybean meal and application thereof |
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