CN114888920B - Method for producing fiber board by hydrophobin - Google Patents

Method for producing fiber board by hydrophobin Download PDF

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CN114888920B
CN114888920B CN202210521471.1A CN202210521471A CN114888920B CN 114888920 B CN114888920 B CN 114888920B CN 202210521471 A CN202210521471 A CN 202210521471A CN 114888920 B CN114888920 B CN 114888920B
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hydrophobin
fiber board
preparing
emulsion
fermentation
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CN114888920A (en
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邬建国
梁小华
金慈
鄢贵龙
王嘉培
王敏
朱敏敏
朱雨萱
朱洁
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Huaiyin Normal University
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
    • B27N—MANUFACTURE BY DRY PROCESSES OF ARTICLES, WITH OR WITHOUT ORGANIC BINDING AGENTS, MADE FROM PARTICLES OR FIBRES CONSISTING OF WOOD OR OTHER LIGNOCELLULOSIC OR LIKE ORGANIC MATERIAL
    • B27N3/00—Manufacture of substantially flat articles, e.g. boards, from particles or fibres
    • B27N3/04—Manufacture of substantially flat articles, e.g. boards, from particles or fibres from fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
    • B27N—MANUFACTURE BY DRY PROCESSES OF ARTICLES, WITH OR WITHOUT ORGANIC BINDING AGENTS, MADE FROM PARTICLES OR FIBRES CONSISTING OF WOOD OR OTHER LIGNOCELLULOSIC OR LIKE ORGANIC MATERIAL
    • B27N1/00—Pretreatment of moulding material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
    • B27N—MANUFACTURE BY DRY PROCESSES OF ARTICLES, WITH OR WITHOUT ORGANIC BINDING AGENTS, MADE FROM PARTICLES OR FIBRES CONSISTING OF WOOD OR OTHER LIGNOCELLULOSIC OR LIKE ORGANIC MATERIAL
    • B27N3/00—Manufacture of substantially flat articles, e.g. boards, from particles or fibres
    • B27N3/002—Manufacture of substantially flat articles, e.g. boards, from particles or fibres characterised by the type of binder
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
    • B27N—MANUFACTURE BY DRY PROCESSES OF ARTICLES, WITH OR WITHOUT ORGANIC BINDING AGENTS, MADE FROM PARTICLES OR FIBRES CONSISTING OF WOOD OR OTHER LIGNOCELLULOSIC OR LIKE ORGANIC MATERIAL
    • B27N3/00—Manufacture of substantially flat articles, e.g. boards, from particles or fibres
    • B27N3/08—Moulding or pressing
    • B27N3/10—Moulding of mats
    • B27N3/12—Moulding of mats from fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/91—Use of waste materials as fillers for mortars or concrete

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Abstract

The invention discloses a method for preparing a fiber board by utilizing hydrophobin, which comprises the following steps: preparation of hydrophobin: inoculating coriolus versicolor seed liquid into the liquid culture medium, and performing submerged liquid fermentation to obtain fermentation liquor rich in hydrophobin; preparing an emulsion: fully stirring the fermentation liquor until the fermentation liquor is emulsified to obtain emulsion; preparing a fiber board: and adding the emulsion into wood processing residues according to a certain proportion, uniformly mixing, and preparing the fiberboard by wet hot pressing. The invention mainly utilizes the coriolus versicolor strain to ferment in a specific culture medium to generate hydrophobin, lignocellulose and bean pulp gum, and the fermentation liquid is completely used for preparing the fiber board after emulsification; the fermentation liquor containing hydrophobin, lignocellulose and bean pulp gum prepared by the method can be specially used for preparing the fiber board, and the prepared fiber board has high strength, low water absorption expansion rate, green and environment-friendly whole production process, no pollution and remarkable economic benefit.

Description

Method for producing fiber board by hydrophobin
Technical Field
The invention relates to the field of fermentation engineering and forestry engineering, in particular to a method for preparing a fiberboard by utilizing hydrophobin.
Background
Hydrophobins are generally composed of about 100 amino acid residues, contain a large number of hydrophobic amino acids and 4 disulfide bonds, and are the most surface-active protein species known to date. The most outstanding characteristics of the novel porous membrane are that the molecular structure has both hydrophobic and hydrophilic parts, and almost any surface can be self-assembled to form a firm and highly ordered amphiphilic membrane, so that fungi can be adsorbed on various surfaces and the novel porous membrane is suitable for various environments.
Hydrophobins are neither cytotoxic nor strongly immunogenic and are therefore considered to be safe in food and medical use. Since hydrophobins can change surface properties, assembling hydrophobins on the surface of medical implants can improve the biocompatibility of these implants or can prevent microbial cells from adhering to the surface of, for example, a catheter, thereby achieving the goal of protecting the implant surface; in the aspect of fruit and vegetable fresh-keeping, the hydrophobin can form a layer of anti-corrosion fresh-keeping film on the surfaces of the fruits and vegetables; hydrophobin can also be used as a component of a cleaning product to form a stable foam; can be used for promoting the degradation of pollutants in soil and being applied to the process of recovering petroleum after petroleum leakage.
The existing strains for producing hydrophobin mainly comprise fungi such as Saccharomyces cerevisiae, trichoderma reesei, flammulina velutipes, grifola frondosa and bacillus subtilis, and the production method mainly comprises a liquid submerged fermentation method and a solid culture method. It was found that the yield of hydrophobin by solid state fermentation was 3.8mg/g, which is 2 times (1.86 mg/g) the yield by submerged liquid fermentation, probably because the environment near the natural growth state was more favorable for the production and secretion of proteins and secondary metabolites in filamentous fungi. Hydrophobin is synthesized by microorganism for convenient use, and can be applied only by complicated and expensive separation and purification processes, and hydrophobin with higher purity is required for application in the fields of medicine and monitoring. The main purification methods of hydrophobin at present comprise a foam fractionation method, a double water phase purification system, a reverse high performance liquid chromatography method and the like, and the higher the purity is, the higher the purification cost is, and the higher load wastewater is produced, so that high production and wastewater treatment costs are brought to enterprises. Therefore, a simple, easy-to-operate and pollution-free hydrophobin production technology is an urgent need for water transport proteins at present.
The fiber board is an artificial board made of wood fiber or other plant fiber as raw material and adhesive applied, and is an effective way for comprehensive utilization of wood resources. However, the existing fiberboard is deformed by warping due to expansion force difference after moisture absorption, and has poor water resistance, and a waterproofing agent with high proportion needs to be additionally added. Through incomplete statistics, 30-50 kg of waterproof agent is required to be added to produce the 1-cubic meter fiberboard, and according to the current market price of the waterproof agent, the 1-cubic meter fiberboard production requires more than 400 yuan of waterproof agent addition cost, and the cost is very important in fiberboard enterprises with low profit margin.
The molecular structure of the hydrophobin has both hydrophobic and hydrophilic parts, whether the hydrophilic parts are favorable for chemical adhesion of the fiber board, whether the hydrophobic parts are favorable for water resistance of the fiber board, and no related research on the aspects of the hydrophobin and no report on the application of the hydrophobin to the production of the fiber board exist at present.
Disclosure of Invention
The invention aims to: aiming at the problems in the prior art, the invention provides a method for preparing a fiberboard by using hydrophobin, and the fermentation liquor prepared by the method and containing hydrophobin, lignocellulose and bean pulp gum can be specially used for preparing the fiberboard, and the prepared fiberboard has high strength, low water absorption expansion rate, green and environment-friendly whole production process, no pollution and obvious economic benefit and can effectively solve the problems in the background art.
The technical scheme is as follows: the invention provides a method for preparing a fiber board by utilizing hydrophobin, which comprises the following steps: preparation of hydrophobin: inoculating coriolus versicolor seed liquid into the sterilized liquid culture medium, and performing submerged liquid fermentation to obtain fermentation liquor rich in hydrophobin; preparing an emulsion: fully stirring the fermentation liquor until the fermentation liquor is emulsified to obtain emulsion; preparing a fiber board: and adding the emulsion into wood processing residues according to a certain proportion, uniformly mixing, and preparing the fiberboard by wet hot pressing.
Preferably, the preparation method of the coriolus versicolor seed liquid comprises the following steps: inoculating a coriolus versicolor inclined plane strain onto a potato culture medium inclined plane for activation, culturing until hypha grows to be full of the inclined plane, inoculating a hypha inclined plane block into a potato liquid culture medium for culturing for 4-6 d at 25-30 ℃ at 120-180 r/min, and amplifying the strain to a proper volume step by step according to an inoculation volume percentage of 8-12%, wherein the fermentation condition is 180-220 r/min, and the ventilation rate is 1: and (3) culturing for 3-5 days at the temperature of 25-30 ℃ and 0.6-0.9 to obtain the coriolus versicolor seed liquid.
Preferably, the deep liquid fermentation condition of hydrophobin preparation is 25-30 ℃ and 200-300 r/min, and the ventilation rate is 1: 0.7-1, and fermenting for 5-8 d.
Preferably, the liquid medium contains 0.1-0.5% by mass of lignocellulose powder. The coriolus versicolor can produce lignocellulose under the induction of lignocellulose.
Preferably, the liquid culture medium also comprises 2-8% of soybean meal powder by mass fraction. The coriolus versicolor can utilize secreted lignocellulose to carry out enzymatic modification on the unused soybean meal while utilizing the soybean meal to synthesize hydrophobin, so as to produce the soybean meal glue with stronger gluing property.
Preferably, the liquid culture medium further comprises 2-3% of glucose by mass and 0.2-0.8% of ammonium sulfate by mass.
Preferably, in the step of preparing a fiberboard, the emulsion is added in an amount of 10 to 30% by mass of the wood processing remainder.
Preferably, in the step of preparing the fiberboard, the wood processing remainder is wetted with water, and then the emulsion is added and mixed uniformly.
Preferably, in the step of preparing the emulsion, the fermentation broth is stirred until no granular feel exists, and the emulsion is obtained. The stirring method is not limited.
Preferably, the wood processing residues comprise wood shavings, sawdust and/or shoots. The wood species are not limited.
The beneficial effects are that:
in the step of preparing the fiberboard, the lignocellulose (mainly lignin degrading enzyme system) in the emulsion can activate wood processing residues to generate active free radical ions; hydrophilic groups of hydrophobin and bean pulp glue in bean pulp powder can generate an adhesive reaction with wood processing residues in a hot pressing process, and free radical ions can strengthen the adhesive reaction; the hydrophobic groups of the hydrophobin can make the fiber board have hydrophobicity from inside to outside, so that the water absorption expansion rate of the fiber board is reduced.
1. The fermentation liquor prepared by the invention is specially used for preparing the fiber board. According to the invention, through the unique design of the additive of the coriolus versicolor culture medium, the natural coordination and collocation of lignocellulose, hydrophobin and bean pulp glue in the fermentation liquor are realized, and the prepared fermentation liquor is specially used for preparing the fiber board;
2. the invention is green and environment-friendly, and has no pollution. The production of traditional lignocellulose, hydrophobin and soy protein glue requires additional extraction and treatment, thus producing a large amount of waste and causing environmental pollution; the fermentation liquor obtained by the invention is completely used for preparing the emulsion, and is further completely used for preparing the fiber board, and the whole process has no waste and pollutant, and is environment-friendly.
3. The invention has low production cost for preparing the fiber board. Taking a waterproof agent as an example, the use cost of the waterproof agent is more than 400 yuan per cubic meter of fiberboard in the traditional fiberboard preparation; the invention adopts the coriolus versicolor biological method to prepare the fermentation broth rich in hydrophobin, lignocellulose and bean pulp gum, the production cost per ton is lower than 1000 yuan (the larger the scale is, the lower the production cost is), and the use cost per cubic meter of fiber board is lower than 200-300 yuan. The economic benefit is obvious.
In summary, the invention prepares the fiberboard by fermenting coriolus versicolor in a culture medium containing bean pulp powder and lignocellulose powder to obtain a fermentation liquid, emulsifying the fermentation liquid into an emulsion, and adding the emulsion into wood processing residues. The strength and water resistance of the prepared fiber board are obviously improved, and compared with the traditional fiber board, the preparation method has obvious economic benefit.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Seed liquid for cultivating coriolus versicolor
Inoculating the coriolus versicolor inclined plane strain preserved in a refrigerator onto a potato culture medium inclined plane for activation, culturing until mycelia grow to be full of the inclined plane, inoculating 3 mycelia inclined plane blocks into 100mL potato liquid culture medium for culturing, culturing for 5d at 28 ℃ at 150r/min, and gradually amplifying to a proper volume according to 10% of inoculation volume percentage, wherein the fermentation condition is 200r/min, and the ventilation rate is 1: culturing at 28deg.C for 4d at 0.8 to obtain coriolus versicolor seed liquid.
Example 1 preparation of fiberboard Using hydrophobin
The formula of the liquid culture medium comprises: glucose 2%, soybean meal 5%, ammonium sulfate 0.5% and lignocellulose powder 0.3%. Sterilizing the liquid culture medium at 121deg.C for 20min, cooling, inoculating cultured Coriolus versicolor seed liquid, fermenting at 28deg.C in a 5 ton fermenter with air volume of 1:0.8, and fermenting for 6d to obtain fermentation liquid. Through detection, the content of hydrophobin in the fermentation liquid is 15.2mg/L, and laccase, cellulase and xylose with higher activity can be detected. Stirring and emulsifying the fermentation liquid by a high-speed wall breaking machine until no granular sensation exists in the fermentation liquid, and obtaining emulsion. The poplar wood shavings are firstly wetted by clear water, then added with emulsion according to the mass ratio of 20 percent, stirred uniformly and pressed into fiber boards by adopting the traditional hot pressing plate making technology. The bending strength of the fiber board reaches 27.6MPa, and the water absorption expansion rate is 13.2%.
Example 2 preparation of fiberboard Using hydrophobin
The formula of the liquid culture medium comprises: glucose 3%, soybean meal 2%, ammonium sulfate 0.8% and lignocellulose powder 0.5%. Sterilizing the liquid culture medium at 121deg.C for 20min, cooling, inoculating cultured Coriolus versicolor seed liquid, fermenting at 25deg.C in a 5 ton fermenter with air volume of 1:0.7, and fermenting for 5d to obtain fermentation liquid. Through detection, the content of hydrophobin in the fermentation liquid is 12.6mg/L, and laccase, cellulase and xylose with higher activity can be detected. Stirring and emulsifying the fermentation liquid by a soymilk machine until no granular feel exists in the fermentation liquid, and obtaining emulsion. The poplar wood shavings are firstly wetted by clear water, then added with emulsion according to the mass ratio of 30 percent, stirred uniformly and pressed into fiber boards by adopting the traditional hot pressing board making technology. The bending strength of the fiber board reaches 29.2MPa, and the water absorption expansion rate is 12.4%.
Example 3 preparation of fiberboard Using hydrophobin
The formula of the liquid culture medium comprises: glucose 2%, soybean meal 8%, ammonium sulfate 0.2% and lignocellulose powder 0.1%. Sterilizing the liquid culture medium at 121deg.C for 20min, cooling, inoculating cultured Coriolus versicolor seed liquid, fermenting at 30deg.C in a 5 ton fermenter with air volume of 300r/min maintained at 1:1, and fermenting for 8d to obtain fermentation broth. Through detection, the content of hydrophobin in the fermentation liquid is 18.7mg/L, and laccase, cellulase and xylose with higher activity can be detected. Stirring and emulsifying the fermentation liquid by a high-speed wall breaking machine until no granular sensation exists in the fermentation liquid, and obtaining emulsion. The poplar wood shavings are firstly wetted by clear water, then emulsion is added according to the mass ratio of 10 percent, the mixture is stirred uniformly, and a traditional hot pressing plate making process is adopted to press the fiberboard. The bending strength of the fiber board reaches 26.3MPa, and the water absorption expansion rate is 15.1%.
Comparative example 1 preparation of fiberboard directly using poplar wood shavings
Pulverizing poplar wood shavings to 60 mesh with pulverizer, adding tap water with certain volume to control water content to 60%, and hot-pressing to obtain fiber board by conventional wet pressing method. The bending strength and the water-absorbing expansion rate of the prepared fiber board are respectively 12.5MPa and 35.3 percent.
Comparative example 2 preparation of fiberboard Using only coriolus versicolor seed liquid
Breaking the wall of the coriolus versicolor seed liquid by adopting a high-speed wall breaking machine, and stirring until emulsification is carried out until the seed liquid has no granular feel, thus obtaining the coriolus versicolor emulsion. The poplar wood shavings are firstly wetted by clear water, then mixed with coriolus versicolor emulsion according to the mass ratio of 10%, stirred uniformly and pressed into fiber boards by adopting the traditional hot pressing plate making technology. The bending strength of the fiber board reaches 17.7MPa, and the water absorption expansion rate is 31.5%.
The foregoing embodiments are merely illustrative of the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the present invention and to implement the same, not to limit the scope of the present invention. All equivalent changes or modifications made according to the spirit of the present invention should be included in the scope of the present invention.

Claims (7)

1. A method for preparing a fiber board by using hydrophobin, which is characterized in that: the method comprises the following steps:
preparation of hydrophobin: inoculating coriolus versicolor seed liquid into the sterilized liquid culture medium, and performing submerged liquid fermentation to obtain fermentation liquor rich in hydrophobin, lignocellulose and bean pulp gum; the conditions of the submerged liquid fermentation are as follows: 25-30 ℃ and 200-300 r/min, and the ventilation rate is 1: 0.7-1, fermenting for 5-8 d; the liquid culture medium comprises 0.1-0.5% of lignocellulose powder and 2-8% of soybean meal powder by mass fraction; the method comprises the steps that lignocellulose is produced by the coriolus versicolor under the induction of lignocellulose, the coriolus versicolor utilizes bean pulp powder to synthesize hydrophobin, and meanwhile, the secreted lignocellulose is utilized to carry out enzymatic modification on the unused bean pulp powder, so that the bean pulp glue with strong gluing property is produced;
preparing an emulsion: fully stirring the fermentation liquor until the fermentation liquor is emulsified to obtain emulsion;
preparing a fiber board: adding the emulsion into wood processing residues according to a certain proportion, uniformly mixing, and preparing a fiberboard by wet hot pressing; the lignocellulose in the emulsion activates wood processing residues to generate active free radical ions, hydrophilic groups of hydrophobin and bean pulp glue in bean pulp powder can generate gluing reaction with the wood processing residues in the hot pressing process, and the free radical ions can strengthen the gluing reaction.
2. The method for producing a fiber board using hydrophobin according to claim 1, wherein: the preparation method of the coriolus versicolor seed liquid comprises the following steps:
inoculating a coriolus versicolor inclined plane strain onto a potato culture medium inclined plane for activation, culturing until hypha grows to be full of the inclined plane, inoculating a hypha inclined plane block into a potato liquid culture medium for culturing, shake culturing for 4-6 d at 25-30 ℃ at 120-180 r/min, and amplifying to a proper volume step by step according to an inoculation volume percentage of 8-12%, wherein the fermentation condition is 180-220 r/min, and the ventilation rate is 1: and (3) culturing for 3-5 days at the temperature of 25-30 ℃ and 0.6-0.9 to obtain the coriolus versicolor seed liquid.
3. The method for producing a fiber board using hydrophobin according to claim 1, wherein: the liquid culture medium also comprises 2-3% of glucose by mass and 0.2-0.8% of ammonium sulfate by mass.
4. The method for producing a fiber board using hydrophobin according to claim 1, wherein: in the step of preparing the fiberboard, the added emulsion accounts for 10-30% of the mass of the wood processing residues.
5. The method for producing a fiber board using hydrophobin according to claim 1, wherein: in the step of preparing the fiberboard, the wood processing residues are firstly wetted by water, then the emulsion is added, and the mixture is uniformly mixed.
6. The method for producing a fiber board using hydrophobin according to any one of claims 1 to 5, wherein: in the step of preparing the emulsion, the fermentation broth is stirred until the hand feeling is free from granular feel, and the emulsion is obtained.
7. The method for producing a fiber board using hydrophobin according to any one of claims 1 to 5, wherein: the wood processing residues include wood shavings, sawdust and/or shoots.
CN202210521471.1A 2022-05-13 2022-05-13 Method for producing fiber board by hydrophobin Active CN114888920B (en)

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CN102650108B (en) * 2012-04-19 2014-06-04 华中科技大学 Method for producing fiberboards by lignocelluloses as raw materials
CN105624133A (en) * 2016-01-20 2016-06-01 徐向群 Technique for producing Inonotus obliquus lignocellulose catabolic enzyme by liquid submerged fermentation
CN106634818A (en) * 2016-12-27 2017-05-10 常州大学 Preparation method of biological protein-based water-tolerant wood adhesive
CN107457880A (en) * 2017-09-15 2017-12-12 淮阴师范学院 Utilize the method for white rot mycelium production non-glue fibre board
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