CN114798018B - Multifunctional microfluidic chip - Google Patents
Multifunctional microfluidic chip Download PDFInfo
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- CN114798018B CN114798018B CN202210221239.6A CN202210221239A CN114798018B CN 114798018 B CN114798018 B CN 114798018B CN 202210221239 A CN202210221239 A CN 202210221239A CN 114798018 B CN114798018 B CN 114798018B
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- 239000007788 liquid Substances 0.000 claims abstract description 651
- 238000001514 detection method Methods 0.000 claims abstract description 113
- 238000003860 storage Methods 0.000 claims abstract description 84
- 238000002156 mixing Methods 0.000 claims abstract description 61
- 238000006243 chemical reaction Methods 0.000 claims abstract description 47
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 27
- 238000003825 pressing Methods 0.000 claims description 40
- 239000002699 waste material Substances 0.000 claims description 26
- 238000011084 recovery Methods 0.000 claims description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 26
- 239000012530 fluid Substances 0.000 description 22
- 239000000243 solution Substances 0.000 description 18
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention discloses a multifunctional microfluidic chip, which comprises a detection assembly, wherein the detection assembly comprises a rotary valve body and an auxiliary liquid inlet chip body, a liquid mixing chip body is fixed at the lower side of one end of the auxiliary liquid inlet chip body in the left-right direction, a first liquid storage module and at least one group of second liquid storage modules are arranged on the auxiliary liquid inlet chip body, the rotary valve body is rotatably connected to the auxiliary liquid inlet chip body and the liquid mixing chip body, a liquid mixing flow channel is arranged in the liquid mixing chip body, a reaction liquid outlet groove is arranged at the downward end of the liquid mixing chip body, a first liquid outlet control module is arranged between the first liquid storage module and the rotary valve body, and the first liquid outlet control module and the rotary valve body jointly control the on-off of liquid flowing out from the first liquid storage module and flowing into the liquid mixing flow channel; the reaction chip body is provided with a reaction module corresponding to the reaction liquid outlet groove at one upward end; the invention can realize the detection of different markers according to the actual detection requirement.
Description
Technical Field
The invention relates to the technical field of nucleic acid detection, in particular to a multifunctional microfluidic chip.
Background
The microfluidic technology can integrate basic operation units such as sample preparation, reaction, separation and the like in the biochemical analysis process on a chip, automatically complete the analysis process, has the advantages of low sample consumption, high detection speed, simplicity and convenience in operation and the like, and is often used in marker detection work in the medical field so as to realize diagnosis of related diseases. The prior micro-fluidic chip comprises an upper chip body, wherein the lower side of the upper chip body is fixedly provided with a middle chip body, the lower side of the middle chip body is fixedly provided with a lower chip body, the middle chip body is often used for solution reaction, the lower chip body is often used for recycling waste liquid, the upper chip body is used for realizing storage of liquid and feeding liquid according to the sequence of liquid feeding, the micro-fluidic chip is only provided with one detection system, multiple detection systems cannot be established through the structure of one chip, one disease corresponds to one chip structure, and the application range is small.
Disclosure of Invention
This section is intended to outline some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description summary and in the title of the application, to avoid obscuring the purpose of this section, the description summary and the title of the invention, which should not be used to limit the scope of the invention.
The present invention has been made in view of the above and/or problems occurring in the prior art of microfluidics.
Therefore, the invention aims to provide a multifunctional microfluidic chip which can realize various detection systems and has wider application range.
In order to solve the technical problems, the invention provides the following technical scheme: a multifunctional microfluidic chip comprises,
the detection assembly comprises a rotary valve body and an auxiliary liquid inlet chip body, wherein the lower side of one end of the auxiliary liquid inlet chip body in the left-right direction is fixedly provided with a liquid mixing chip body, a first liquid storage module and at least one group of second liquid storage modules are arranged on the auxiliary liquid inlet chip body, a rotating hole is formed between the auxiliary liquid inlet chip body and the liquid mixing chip body, the rotary valve body is rotatably connected to the auxiliary liquid inlet chip body and the liquid mixing chip body through the rotating hole, a liquid mixing flow channel is formed in the liquid mixing chip body, a reaction liquid outlet groove is formed in one downward end of the liquid mixing chip body, a first liquid outlet control module and a second liquid outlet control module are respectively arranged in the auxiliary liquid inlet chip body and the liquid mixing chip body between the first liquid storage module and the rotary valve body, the first liquid outlet control module and the rotary valve body jointly control the on-off of liquid flowing out of the first liquid storage module and the liquid mixing flow channel, and the second liquid outlet control module and the rotary valve body jointly control the on-off of liquid flowing out of the second liquid storage module and the liquid inlet end of the reaction liquid outlet channel;
The reaction chip comprises a reaction chip body, wherein one upward end of the reaction chip body is provided with a reaction module corresponding to a reaction liquid outlet groove, the reaction module comprises a plurality of reaction tanks which are arranged on the reaction chip body, the reaction liquid outlet groove covers the corresponding reaction module, and the reaction chip body is arranged on the lower side of the liquid mixing chip body;
the waste liquid recovery chip body, waste liquid recovery chip body one end up is equipped with the waste liquid pond, the waste liquid pond sets up the play liquid end at the reaction play cistern, waste liquid recovery chip body is in the downside of reaction chip body.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the first liquid storage module comprises a plurality of first liquid storage tanks which are arranged in the auxiliary liquid inlet chip body, first liquid inlet channels which are the same as the first liquid storage tanks in number are arranged in the liquid mixing chip body close to one end of the rotary valve body, the liquid mixing chip body is rotatably connected with a first motor valve body, a liquid outlet which can cover one end of the liquid mixing channel is formed in the first motor valve body, a plurality of liquid inlet holes which are in one-to-one correspondence with the first liquid inlet channels are also arranged in the first motor valve body, and when the liquid inlet holes cover the corresponding first liquid inlet channels, the liquid outlet just covers one end of the liquid mixing channel.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the other end of the liquid mixing flow passage can be communicated with the liquid inlet end of the reaction liquid outlet groove.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the liquid mixing chip body close to one end of the rotary valve body is internally provided with a plurality of second liquid inlet channels corresponding to the second liquid storage modules, the number of the second liquid inlet channels is the same as that of the second liquid storage tanks in the second liquid storage modules, the liquid mixing chip body is rotatably connected with a second motor valve body corresponding to the second liquid storage modules, the second motor valve body is also provided with a plurality of liquid inlet holes corresponding to the second liquid inlet channels one by one, the liquid mixing chip body close to the rotary valve body is internally provided with a plurality of second liquid inlet channels corresponding to the second liquid storage tanks in number, and when the liquid inlet holes on the second motor valve body cover the corresponding second liquid inlet channels, the liquid outlet on the second motor valve body covers the liquid inlet end of the reaction liquid outlet tank.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the auxiliary liquid inlet chip body is provided with first control holes corresponding to the first liquid storage modules, the auxiliary liquid inlet chip body is connected with a first pressing valve body capable of lifting through the first control holes, and different first liquid storage tanks are respectively communicated with the first liquid outlet control modules through lifting the first pressing valve body.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the auxiliary liquid inlet chip body between the three first liquid storage tanks and the first control holes is respectively provided with a first flow channel, a second flow channel and a third flow channel, the first pressing valve body is sequentially provided with a first through hole which can be communicated with the first flow channel, a second through hole which can be communicated with the second flow channel and a third through hole which can be communicated with the third flow channel from bottom to top, the first liquid outlet control module comprises a first upper control liquid flow channel, a second upper control liquid flow channel and a third upper control liquid flow channel which are arranged in the auxiliary liquid inlet chip body and between the first control holes and the rotary valve body, one end of the first through hole, which is far away from the first flow channel, can be communicated with one end of the first upper control liquid flow channel, one end of the second through hole, which is far away from the third flow channel, can be communicated with one end of the third upper control liquid flow channel, and the second upper control liquid flow channel can be respectively communicated with the first liquid inlet flow channel through the rotary valve.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the first liquid outlet control module further comprises a first lower control liquid outlet channel arranged in the auxiliary liquid inlet chip body, the upper end of the first lower control liquid outlet channel is communicated with the first upper control liquid outlet channel, and a first upper liquid outlet control valve and a first lower liquid outlet control valve are respectively arranged in the auxiliary liquid inlet chip body at the first upper control liquid outlet channel and the first lower control liquid outlet channel.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the first liquid control module is characterized in that the first liquid control module further comprises a second middle control liquid outlet channel and a third middle control liquid outlet channel which are arranged in the auxiliary liquid inlet chip body, the upper end of the second middle control liquid outlet channel is communicated with the second upper control liquid outlet channel, the upper end of the third middle control liquid outlet channel is communicated with the third upper control liquid outlet channel, the second lower control liquid outlet channel, the third middle control liquid outlet channel and the third lower control liquid outlet channel are arranged in the liquid mixing chip body, the upper end of the second lower control liquid outlet channel is communicated with the second middle control liquid outlet channel, the other ends of the second middle control liquid outlet channel and the second lower control liquid outlet channel are respectively communicated with the corresponding first liquid inlet channel through a rotary valve body, the upper end of the third lower control liquid outlet channel is communicated with the third middle control liquid outlet channel, the other ends of the third middle control liquid outlet channel and the third lower control liquid outlet channel are respectively communicated with the corresponding first liquid inlet channel through the rotary valve body, the second upper control liquid outlet channel and the second middle control liquid outlet channel are respectively arranged in the auxiliary liquid inlet chip body at the second upper control liquid outlet channel and the second middle control liquid outlet channel, the second middle control liquid outlet channel is respectively communicated with the third middle control liquid outlet channel is respectively arranged in the second middle liquid outlet chip body, the second middle control liquid outlet channel is respectively provided with the second middle liquid outlet channel is respectively.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the auxiliary liquid inlet chip body is provided with a second control hole corresponding to the second liquid storage module, the auxiliary liquid inlet chip body is connected with a second pressing valve body capable of lifting through the second control hole, and different second liquid storage tanks are communicated with the second liquid outlet control module through lifting the second pressing valve body.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the second pressing valve body and the first pressing valve body are identical in structure, and the first liquid outlet control module and the second liquid outlet control module are identical in structure.
The invention has the beneficial effects that: according to the invention, through the arrangement of the second pressing valve body, the reagents in the first liquid storage tank and the second liquid storage tank are controlled to sequentially discharge according to the requirement; through the joint setting of first play liquid control module, second play liquid control module and rotatory valve body, can realize the detection of different markers according to actual detection needs, application scope is wider.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:
Fig. 1 is a perspective view of the present invention.
Fig. 2 is a three-dimensional structure diagram of the present invention when two ends of a first upper detection liquid outlet channel are respectively communicated with a first upper control liquid outlet channel and a liquid inlet channel, two ends of a second upper detection liquid outlet channel are respectively communicated with a second upper control liquid outlet channel and a liquid inlet channel, and two ends of a third upper detection liquid outlet channel are respectively communicated with a third upper control liquid outlet channel and a liquid inlet channel.
Fig. 3 is a partial enlarged view at a in fig. 2.
Fig. 4 is a partial enlarged view at B in fig. 2.
Fig. 5 is a partial enlarged view at C in fig. 2.
Fig. 6 is a perspective view of the present invention when two ends of a first lower detection liquid outlet channel are respectively connected to a first lower control liquid outlet channel and a liquid inlet channel, and two ends of a third middle detection liquid outlet channel are respectively connected to a second middle control liquid outlet channel and a liquid inlet channel.
Fig. 7 is a partial enlarged view at D in fig. 6.
Fig. 8 is a perspective view of the present invention when two ends of a second middle detection liquid outlet channel are respectively connected to a second middle control liquid outlet channel and a liquid inlet channel, and two ends of a third lower detection liquid outlet channel are respectively connected to a third lower control liquid outlet channel and a liquid inlet channel.
Fig. 9 is a partial enlarged view at E in fig. 8.
Fig. 10 is a perspective view of the structure of the present invention when two ends of the second lower detection liquid outlet channel are respectively connected to the second lower control liquid outlet channel and the liquid inlet channel.
Fig. 11 is a partial enlarged view of F in fig. 10.
Fig. 12 is a partial enlarged view of G in fig. 10.
Fig. 13 is a perspective view of a reaction chip body according to the present invention.
FIG. 14 is a perspective view of the waste liquid recovery chip body according to the present invention.
In the figure, a 100 waste liquid recovery chip body, a 101 waste liquid pond, a 200 reaction chip body, a 201 reaction pond, a 202 waste liquid hole, a 300 driving motor, a 400 first pressing valve body, a 500 second pressing valve body, a 600 auxiliary liquid inlet chip body, a 601 first injection port, a 602 second injection port, a 603 first liquid outlet control module, a 603a first upper control liquid outlet channel, a 603b first upper liquid outlet control valve, a 603c second intermediate control liquid outlet channel, a 603d second upper control liquid outlet channel, a 603e second upper liquid outlet control valve, a 603f third upper control liquid outlet channel, a 603g third upper liquid outlet control valve, a 603h first lower liquid outlet control valve, a 603i first lower control liquid outlet channel, a 603j second lower control liquid outlet channel, a 604 first liquid storage tank, a 605 second liquid storage module, a 605 second liquid storage tank, a 606 first liquid channel, a 607 second liquid channel, a 608 third liquid outlet channel, 609 second fluid control module, 700 fluid mixing chip body, 701 fluid mixing channel, 702 first fluid inlet channel, 703 second connecting channel, 704 second fluid inlet channel, 705 reaction fluid outlet channel, 706 first connecting channel, 800 rotary valve body, 801 first detection fluid outlet module, 801a first fluid passing component, 801a-1 first upper detection fluid outlet channel, 801a-2 first lower detection fluid outlet channel, 801b third fluid passing component, 801b-1 third lower detection fluid outlet channel, 801b-2 third intermediate detection fluid outlet channel, 801b-3 third upper detection fluid outlet channel, 801c second fluid passing component, 801 c-1 second upper detection fluid outlet channel, 801 c-2 second intermediate detection fluid outlet channel, 801 c-3 second lower detection fluid outlet channel, 802 second detection fluid outlet module, 900 second motor valve body, 901 fluid outlet, 902 control fluid orifice, 903 fluid inlet orifice, 904 negative pressure channel, 905, 906 turn sink, 1000 first motor valve body.
Detailed Description
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present invention is not limited to the specific embodiments disclosed below.
Further, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic can be included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1
Referring to fig. 1, fig. 4, fig. 5, fig. 13 and fig. 14, in order to provide a first embodiment of the present invention, the present embodiment provides a multifunctional microfluidic chip, which has a simple and reliable structure and can improve detection efficiency.
The multifunctional microfluidic chip comprises a detection assembly, wherein the detection assembly comprises a rotary valve body 800 and an auxiliary liquid inlet chip body 600, a mixed liquid chip body 700 is fixed at the lower side of one end of the auxiliary liquid inlet chip body 600 in the left-right direction, a first liquid storage module 604 and at least one group of second liquid storage modules 605 are arranged on the auxiliary liquid inlet chip body 600, a rotary hole is formed between the auxiliary liquid inlet chip body 600 and the mixed liquid chip body 700, the rotary valve body 800 is rotatably connected to the auxiliary liquid inlet chip body 600 and the mixed liquid chip body 700 through the rotary hole, a mixed liquid flow channel 701 is arranged in the mixed liquid chip body 700, a reaction liquid outlet groove 705 is formed at the downward end of the mixed liquid chip body 700, a first liquid outlet control module 603 and a second liquid outlet control module 609 are respectively arranged between the first liquid storage module 604 and the rotary valve body 800, the auxiliary liquid inlet chip body 600 and the mixed chip body between the second liquid storage module 605 and the rotary valve body 800, the first liquid outlet control module 603 and the rotary valve body 800 jointly control the on-off of liquid flowing out of the first liquid storage module 604 into the mixed liquid flow channel 701, and the on-off of the liquid flowing out of the liquid storage channel 705 are jointly controlled by the second liquid control module 609 and the rotary valve body 800;
The reaction chip body 200, one end of the reaction chip body 200 facing upwards is provided with a reaction module corresponding to the reaction liquid outlet tank 705, the reaction module comprises a plurality of reaction tanks 201 arranged on the reaction chip body 200, the reaction liquid outlet tank 705 covers the corresponding reaction module, the reaction chip body 200 at one end of the reaction module far away from the second motor valve body 900 is provided with a waste liquid hole 202, and the reaction chip body 200 is arranged at the lower side of the liquid mixing chip body 700;
the waste liquid recovery chip body 100, the one end that waste liquid recovery chip body 100 upwards is equipped with waste liquid pond 101, and waste liquid pond 101 covers all waste liquid hole 202 positions, and waste liquid pond 101 sets up the liquid end at reaction play cistern 705, and waste liquid recovery chip body 100 is in the downside of reaction chip body 200.
Further, the first liquid storage module 604 includes a plurality of first liquid storage tanks 604a arranged in the auxiliary liquid inlet chip body 600, a first liquid inlet flow channel 702 with the same number as the first liquid storage tanks 604a is arranged in the liquid mixing chip body 700 close to one end of the rotary valve body 800, the liquid mixing chip body 700 is rotatably connected with a first motor valve body 1000, a liquid outlet 901 capable of covering one end of the liquid mixing flow channel 701 is arranged on the first motor valve body 1000, a plurality of liquid inlet holes 903 in one-to-one correspondence with the first liquid inlet flow channel 702 are also arranged on the first motor valve body 1000, when the liquid inlet holes 903 cover the corresponding first liquid inlet flow channels 702, the liquid outlet 901 just covers one end of the liquid mixing flow channel 701, and the other end of the liquid mixing flow channel 701 can be communicated with the liquid inlet end of the reaction liquid outlet tank 705.
Further, a plurality of second liquid inlet channels 704 corresponding to the second liquid storage modules 605 are further arranged in the liquid mixing chip body 700 close to one end of the rotary valve body 800, the number of the second liquid inlet channels 704 is the same as that of the second liquid storage tanks 605a in the second liquid storage modules 605, the second motor valve body 900 corresponding to the second liquid storage modules 605 is rotatably connected to the liquid mixing chip body 700, a plurality of liquid inlet holes 903 corresponding to the second liquid inlet channels 704 one by one are also arranged on the second motor valve body 900, a plurality of second liquid inlet channels 704 corresponding to the second liquid storage tanks 605a in number are arranged in the liquid mixing chip body 700 close to the rotary valve body 800, and when the liquid inlet holes 903 on the second motor valve body 900 cover the corresponding second liquid inlet channels 704, the liquid outlet 901 on the second motor valve body 900 covers the liquid inlet ends of the liquid reaction outlet tanks 705.
Further, a first control hole corresponding to the first liquid storage module 604 is arranged on the auxiliary liquid inlet chip body 600, the auxiliary liquid inlet chip body 600 is connected with a first pressing valve body 400 capable of lifting through the first control hole, different first liquid storage tanks 604a are respectively communicated with the first liquid outlet control module 603 by lifting the first pressing valve body 400, the first liquid storage tanks 604a are provided with three upper control liquid channels 603d and 603f, a first flow channel 606, a second flow channel 607 and a third flow channel 608 are respectively arranged in the auxiliary liquid inlet chip body 600 between the first liquid storage tanks 604a and the first control hole, a first through hole capable of being communicated with the first flow channel 606, a second through hole capable of being communicated with the second flow channel 607 and a third through hole capable of being communicated with the third flow channel 608 are sequentially arranged on the first pressing valve body 400 from bottom to top, the first upper control channel 603 is further provided with a first end capable of being communicated with the first end 603d, which is far from the first end 603d capable of being communicated with the first end 603, and the third end 603f which is far from the first end 603, and the upper control liquid flow channel 603 is far from the first end 603 is communicated with the third end 603 f.
Further, the first liquid outlet control module 603 further includes a first lower control liquid outlet channel 603i disposed in the auxiliary liquid inlet chip body 600, the upper end of the first lower control liquid outlet channel 603i is communicated with the first upper control liquid outlet channel 603a, and the auxiliary liquid inlet chip body 600 at the first upper control liquid outlet channel 603a and the first lower control liquid outlet channel 603i is respectively provided with a first upper liquid outlet control valve 603b and a first lower liquid outlet control valve 603h.
Further, the first liquid control module 603 further includes a second intermediate control liquid outlet channel 603c and a third intermediate control liquid outlet channel which are disposed in the auxiliary liquid inlet chip body 600, the upper end of the second intermediate control liquid outlet channel 603c is communicated with the second upper control liquid outlet channel 603d, the upper end of the third intermediate control liquid outlet channel is communicated with the third upper control liquid outlet channel 603f, a second lower control liquid outlet channel 603j, a third intermediate control liquid outlet channel and a third lower control liquid outlet channel are disposed in the liquid mixing chip body 700, the upper end of the second lower control liquid outlet channel 603j is communicated with the second intermediate control liquid outlet channel 603c, the other ends of the second intermediate control liquid outlet channel 603c and the second lower control liquid outlet channel 603j are respectively communicated with the second intermediate control liquid outlet channel 702 through the rotary valve 800, the upper end of the third intermediate control liquid outlet channel is communicated with the third intermediate control liquid outlet channel 603f, the other ends of the third intermediate control liquid outlet channel and the third intermediate control liquid outlet channel are respectively communicated with the first liquid outlet channel 603f through the rotary valve 800 and the corresponding first liquid outlet channel 603f, the second upper end of the second intermediate control liquid outlet channel 603d and the second intermediate control liquid outlet channel 603c is respectively communicated with the second intermediate control liquid outlet channel 603c, an auxiliary liquid outlet valve is disposed in the second intermediate control liquid outlet channel body 600 g and the third intermediate control liquid outlet channel 603c is respectively communicated with the third intermediate control liquid outlet channel 700.
Further, a second control hole corresponding to the second liquid storage module 605 is formed in the auxiliary liquid inlet chip body 600, the auxiliary liquid inlet chip body 600 is connected with a second pressing valve body 500 capable of lifting through the second control hole, the second pressing valve body 500 has the same structure as the first pressing valve body 400, the first liquid outlet control module 603 has the same structure as the second liquid outlet control module 609, and different second liquid storage tanks 605a are communicated with the second liquid outlet control module 609 by lifting the second pressing valve body 500.
Further, the other ends of the first upper control liquid outlet channel 603a, the first middle control liquid outlet channel, the second upper control liquid outlet channel 603d, the second middle control liquid outlet channel 603c, the second lower control liquid outlet channel 603j, the third upper control liquid outlet channel 603f, the third middle control liquid outlet channel and the third lower control liquid outlet channel are all attached to the outer side of the rotary valve body 800.
The upward ends of the first motor valve body 1000 and the second motor valve body 900 are respectively provided with a rotary sinking groove 906, a first injection port 601 for injecting solution into the first liquid storage tank 604a is arranged on the auxiliary liquid inlet chip body 600 at the first liquid storage tank 604a, and a second injection port 602 for injecting solution into the second liquid storage tank 605a is arranged on the auxiliary liquid inlet chip body 600 at the second liquid storage tank 605 a; according to the liquid inlet sequence, the first pressing valve body 400 is pressed downwards, when the first through hole of the first pressing valve body 400 is communicated with the first flow channel 606, the reagent in the corresponding first liquid storage tank 604a flows into the first upper control liquid flow channel 603a and the first middle control liquid flow channel before the first upper liquid outlet control valve 603b and the first middle control liquid flow channel before the first lower liquid outlet control valve 603h and the first lower control liquid flow channel 603i sequentially through the first flow channel 606 and the first through hole, by controlling each liquid outlet control valve, the reagent flows into the rotary valve body 800 according to the required sequence and flows into the corresponding first liquid inlet flow channel 702 from the rotary valve body 800, the reagent flowing into the first liquid inlet flow channel 702 flows into the mixed liquid flow channel 701 sequentially through the liquid inlet 903 and the liquid outlet 901 of the first motor valve body 1000, when a proper amount of detection reagent enters the mixed liquid flow channel 701, the first pressing valve body 400 is pressed, the first reservoir 604a is isolated from the first upper control liquid outlet channel, if other detection reagent is needed to be mixed, the first pressing valve body 400 is continuously pressed, when the second through hole of the first pressing valve body 400 is communicated with the second flow channel 607, the reagent in the corresponding first reservoir 604a flows into the second upper control liquid outlet channel 603d and the second middle control liquid outlet channel 603c in front of the second upper liquid outlet control valve 603e and the second lower middle control liquid outlet channel 603j in front of the second lower liquid outlet control valve sequentially through the second flow channel 607 and the second through hole, when a proper amount of detection reagent enters the mixed liquid flow channel 701, the first pressing valve body 400 is pressed, so that the first reservoir 604a and the second upper control liquid outlet channel are isolated, if other detection reagent is needed to be mixed, the first pressing valve body 400 is pressed again, when the third through hole of the first pressing valve body 400 is communicated with the third flow channel 608, the reagent in the corresponding first reservoir 604a flows into the third upper control liquid outlet channel 603f, the third upper middle control liquid outlet channel 603f, the third lower middle control liquid outlet channel and the third lower control liquid outlet channel in front of the third upper liquid outlet control valve 603g through the third flow channel 608 and the third through hole in sequence, when a proper amount of detection reagent enters the mixed liquid channel 701, the first pressing valve body 400 is pressed down to separate the third flow channel 608 from the third upper control liquid outlet channel, so that each detection reagent is mixed in the mixed liquid channel 701, and the reagent after being uniformly mixed through the mixed liquid channel 701 enters the reaction liquid outlet channel 705; storing detection samples, flushing solutions or other detection solutions in different second liquid reservoirs 605a, and controlling each liquid outlet control valve in the second liquid outlet control module 609 according to the liquid outlet sequence, so that the solutions enter the reaction liquid outlet tank 705 according to the set liquid outlet sequence and react with the uniformly mixed detection reagents; according to the utility model, according to the actual requirement, the required detection reagent is injected into the first liquid storage tank 604a, the required reagent flows into the first upper control liquid outlet flow channel 603a, the second upper control liquid outlet flow channel 603d or the third upper control liquid outlet flow channel 603f by pressing the first pressing valve body 400, the mixing of three detection reagents or two detection reagents is realized by controlling each liquid outlet control valve, and the detection of different markers is realized by setting the second pressing valve body 500 and the corresponding liquid outlet control valves.
Example 2
Referring to fig. 12, in a second embodiment of the present invention, a multi-functional microfluidic chip is provided, which can implement the pumping of a reagent in a mixed-liquid channel 701 or a solution in a second liquid outlet control module 609.
The multifunctional microfluidic chip is characterized in that a control liquid hole 902 communicated with a liquid outlet 901 is further formed in the second motor valve body 900, two groups of second liquid storage modules 605 and two groups of second liquid outlet control modules 609 corresponding to the second liquid storage modules 605 one by one are arranged on the auxiliary liquid inlet chip body 600, a first connecting flow channel 706 and a second connecting flow channel 703 are arranged in the liquid mixing chip body 700 between the two second motor valve bodies 900, one end of the first connecting flow channel 706 can be communicated with the control liquid hole 902 on one second motor valve body 900, one end of the second connecting flow channel 703 can be communicated with the control liquid hole 902 of the other second motor valve body 900, and the other end of the first connecting flow channel 706, the other end of the second connecting flow channel 703 and the other end of the liquid mixing flow channel 701 are connected.
Further, a negative pressure connecting pipe 905 is arranged on the outer side of the second motor valve body 900 above the liquid mixing chip body 700, a negative pressure channel 904 is arranged on the second motor valve body 900, one end of the negative pressure channel 904 is communicated with an inner cavity of the negative pressure connecting pipe 905, and the other end of the negative pressure channel 904 is communicated with the liquid outlet 901.
When the device works, a negative pressure pump is connected to a negative pressure connecting pipe 905, the negative pressure pump pumps liquid in the mixed liquid channel 701 to the direction of a reaction liquid outlet groove 705 through the negative pressure connecting pipe 905 and a negative pressure channel 904, and after a proper amount of detection reagent mixed uniformly in the mixed liquid channel 701 enters the reaction liquid outlet groove 705, the first motor valve body 1000 is rotated, so that a liquid inlet hole 903 on the first motor valve body 1000 is staggered with the first liquid inlet channel 702; in this embodiment, by the arrangement of the negative pressure connection pipe 905 and the negative pressure channel 904 on the second motor valve body 900, the reagent is pumped in the direction of the reaction liquid outlet tank 705, and the liquid entering the reaction liquid outlet tank 705 can flow in the direction of the waste liquid tank 101 by the reverse action of the negative pressure pump.
Example 3
Referring to fig. 1 to 14, in a third embodiment of the present invention, a multifunctional microfluidic chip is provided, which has multiple detection systems to implement multiple functional detection.
The multifunctional microfluidic chip further comprises a driving motor 300, the driving motor 300 is in transmission connection with the rotary valve body 800, a first detection liquid outlet module 801 and two groups of second detection liquid outlet modules 802 corresponding to the second liquid outlet control modules 609 one by one are arranged in the rotary valve body 800, the first detection liquid outlet module 801 and the second detection liquid outlet modules 802 are identical in structure, the first detection liquid outlet module 801 comprises a first flow passage assembly 801a, a second flow passage assembly 801c and a third flow passage assembly 801b which are arranged in the rotary valve body 800 and are sequentially arranged at axial positions from front to back, the first flow passage assembly 801a comprises a first upper detection liquid outlet flow passage 801a-1 and a second lower detection liquid outlet flow passage 801c-3 which are arranged at the same axial positions, the second flow passage assembly 801c comprises a second upper detection liquid outlet flow passage 801c-1, a second middle detection liquid outlet flow passage 801c-2 and a second lower detection liquid outlet flow passage 801c-3 which are arranged at the same axial positions, the third flow passage assembly 801b comprises a third upper detection liquid outlet flow passage 801b-3, a third middle detection liquid outlet flow passage 801b-2 and a third first liquid outlet flow passage 702 b-a third liquid outlet flow passage 702 which is sequentially arranged at the same axial positions, and the third middle detection liquid outlet flow passage 801b-2 is a first liquid outlet flow passage 702 b-a third liquid inlet flow passage is sequentially arranged from front.
Further, when one end of the first upper detection liquid outlet channel 801a-1 covers the other end of the first upper control liquid outlet channel 603a, the other end of the first upper detection liquid outlet channel 801a-1 is communicated with one end of the first liquid inlet channel 702 away from the first motor valve body 1000, one end of the second upper detection liquid outlet channel 801c-1 covers the other end of the second upper control liquid outlet channel 603d, the other end of the second upper detection liquid outlet channel 801c-1 is communicated with one end of the second first liquid inlet channel 702 away from the first motor valve body 1000, one end of the third upper detection liquid outlet channel 801b-3 covers the other end of the third upper control liquid outlet channel 603f, and the other end of the third upper detection liquid outlet channel 801b-3 is communicated with one end of the third first liquid inlet channel 702 away from the first motor valve body 1000.
Further, when one end of the first lower detection liquid outlet channel 801a-2 covers the first intermediate control liquid outlet channel, the other end of the first lower detection liquid outlet channel 801a-2 is communicated with one end of the first liquid inlet channel 702 far away from the first motor valve body 1000, one end of the third intermediate detection liquid outlet channel 801b-2 covers the third upper intermediate control liquid outlet channel, and the other end of the third intermediate detection liquid outlet channel 801b-2 is communicated with one end of the third first liquid inlet channel 702 far away from the first motor valve body 1000.
Further, when one end of the second intermediate detection liquid outlet channel 801c-2 covers the other end of the second lower intermediate control liquid outlet channel, the other end of the second intermediate detection liquid outlet channel 801c-2 is communicated with one end of the second first liquid inlet channel 702 far away from the first motor valve body 1000, one end of the third lower detection liquid outlet channel 801b-1 covers the other end of the third lower intermediate control liquid outlet channel, and the other end of the third lower detection liquid outlet channel 801b-1 is communicated with one end of the third first liquid inlet channel 702 far away from the first motor valve body 1000.
Further, when one end of the second lower detection liquid outlet channel 801c-3 covers the other end of the second lower control liquid outlet channel 603j, the other end of the second lower detection liquid outlet channel 801c-3 is communicated with one end of the second first liquid inlet channel 702 away from the first motor valve body 1000.
The first lower detection liquid outlet channel 801a-2, the third lower detection liquid outlet channel 801b-1 or the second lower detection liquid outlet channel 801c-3 of the second detection liquid outlet module 802 are internally coated with detection molecules, such as fluorescent molecules, enzymes and the like; in the initial state, the first upper detection liquid flow channel 801a-1, the second upper detection liquid flow channel 801c-1 and the third upper detection liquid flow channel 801b-3 on the rotary valve body 800 are respectively aligned with one ends of the first upper control liquid flow channel 603a, the second upper control liquid flow channel 603d and the third upper control liquid flow channel 603f, if the nucleic acid is detected, different detection reagents are respectively stored in the three first liquid storage tanks 604a, the detection sample, the cleaning solution and the magnetic beads are respectively injected in the three second liquid storage tanks 605a, the negative pressure pump is connected with the second motor valve body 900 through the negative pressure connecting pipe 905, the first upper liquid control valve 603b, the second upper liquid control valve 603e and the third upper liquid control valve 603g, the first pressing valve body 400 is pressed to enable the first through hole to be communicated with the first flow channel 606, the first upper liquid control valve 603b on the first upper control liquid flow channel 603a is opened, after a proper amount of detection reagent flows into the first upper control liquid outlet channel 603a, the first pressing valve body 400 is continuously pressed down to enable the second through hole to be communicated with the second flow channel 607, the detection reagent in the corresponding first liquid storage tank 604a flows into the second upper control liquid outlet channel 603d, then the first pressing valve body 400 is pressed down to enable the third through hole to be communicated with the third flow channel 608, the detection reagent in the corresponding first liquid storage tank 604a flows into the third upper control liquid outlet channel 603f, the negative pressure pump acts, three detection reagents respectively enter the liquid mixing channel 701 through the first upper detection liquid outlet channel 801a-1, the second upper detection liquid outlet channel 801c-1 and the third upper detection liquid outlet channel 801b-3 to be mixed and then pumped into the reaction liquid outlet channel 705, the second pressing valve body 500 and the corresponding second upper liquid outlet control valve 603e are controlled to act, the solution in the second storage tank is discharged according to the detection sequence, and the negative pressure pump is combined, pumping the corresponding solution into the reaction liquid outlet tank 705, enabling the solution entering the reaction liquid outlet tank 705 to enter the reaction tank 201, and enabling the redundant solution to enter the waste liquid tank 101 through the waste liquid hole 202; if other markers are detected, when the detection molecules coated in the second lower detection liquid flow channel 801c-3 in the second detection liquid flow module 802 need to be used, the driving motor 300 acts, the rotary valve body 800 rotates, one end of the second lower detection liquid flow channel 801c-3 in the second detection liquid flow module 802 just aims at the second lower control liquid flow channel 603j, the driving motor 300 stops acting, at the moment, the other end of the second lower detection liquid flow channel 801c-3 in the second detection liquid flow module 802 is communicated with the second liquid inlet flow channel 704, the second pressing valve body 500 is pressed to the height corresponding to the second through hole and the second flow channel 607, the second lower liquid outlet control valve in the second control liquid flow module is opened, the liquid inlet hole 903 of the second motor valve body 900 is staggered with the second liquid inlet flow channel 704, a proper amount of reaction solution in the second liquid storage tank 605a reacts with detection molecules coated in the second lower detection liquid flow channel 801c-3, when the second lower detection liquid flow channel 801c-3 in the second detection liquid storage tank 605a flows out from the second liquid storage tank 605a to enter the second lower detection liquid flow channel 801c-3, the second pump is controlled to enable the second liquid outlet valve body to be staggered, the second pump body to rotate, and the second pump is stopped when the second pump is pressed to reach the negative pressure corresponding to the second liquid inlet valve body 900, and the second pump is rotated, the second pump is stopped, and the valve body is rotated to the valve body is positioned to rotate; in the invention, the rotary valve body 800 is rotated to a required position by controlling the action of the driving motor 300, so that a required detection liquid outlet flow channel is aligned with a corresponding control liquid outlet flow channel, and then a reaction solution flows into the reaction liquid outlet tank 705 according to the liquid outlet sequence by controlling the action of the corresponding liquid outlet control valve, and reacts in the reaction tank 201, thereby realizing the detection of different markers, having a plurality of detection systems and having wider application range.
It should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted without departing from the spirit and scope of the technical solution of the present invention, which is intended to be covered in the scope of the claims of the present invention.
Claims (4)
1. The utility model provides a multi-functional micro-fluidic chip which characterized in that: it comprises
The detection assembly comprises a rotary valve body (800) and an auxiliary liquid inlet chip body (600), wherein a liquid mixing channel (701) is arranged in the lower side of one end of the auxiliary liquid inlet chip body (600) in the left-right direction, a first liquid storage module (604) and at least one group of second liquid storage modules (605) are arranged on the auxiliary liquid inlet chip body (600), a rotary hole is arranged between the auxiliary liquid inlet chip body (600) and the liquid mixing chip body (700), the rotary valve body (800) is rotatably connected on the auxiliary liquid inlet chip body (600) and the liquid mixing chip body (700) through the rotary hole, a liquid mixing flow channel (701) is arranged in the liquid mixing chip body (700), a reaction liquid outlet groove (705) is arranged at one end of the liquid mixing chip body (700) facing downwards, a first liquid storage module (604) and a second liquid storage module (605) are arranged between the rotary valve body (800) and the auxiliary liquid inlet chip body (600) and the liquid mixing chip body (700), a first liquid outlet control module (603) and a second liquid outlet control module (609) are respectively arranged in the liquid mixing chip body (700), the first liquid outlet control module (603) and the first liquid outlet control module (603) are connected with the first liquid outlet control module (604) through the rotary valve body (800), the second liquid outlet control module (609) and the rotary valve body (800) jointly control the on-off of the liquid flowing out of the second liquid storage module (605) in the liquid inlet end of the reaction liquid outlet groove (705); the first liquid storage module (604) comprises a plurality of first liquid storage tanks (604 a) which are arranged in the auxiliary liquid inlet chip body (600), first liquid inlet flow passages (702) which are the same as the first liquid storage tanks (604 a) in number are arranged in the liquid mixing chip body (700) which is close to one end of the rotary valve body (800), the first motor valve body (1000) is rotatably connected to the liquid mixing chip body (700), a liquid outlet (901) which can cover one end of the liquid mixing flow passages (701) is arranged on the first motor valve body (1000), a plurality of liquid inlet holes (903) which are in one-to-one correspondence with the first liquid inlet flow passages (702) are also arranged on the first motor valve body (1000), when the liquid inlet holes (903) cover the corresponding first liquid inlet flow passages (702), the liquid outlet (901) just covers one end of the liquid mixing flow passages (701), the other end of the liquid mixing flow passages (701) can be communicated with the liquid inlet ends of the reaction liquid outlet grooves (705), the auxiliary liquid inlet chip body (600) is provided with the first liquid storage chip body (604) which is arranged on the first liquid storage chip body (600) and the first liquid inlet control module (400) which is not communicated with the first liquid storage module (400) through the first liquid inlet control valve body (400) in a pressing mode; the first liquid storage pool (604 a) is provided with three auxiliary liquid inlet chip bodies (600) between the three first liquid storage pools (604 a) and the first control holes, a first flow channel (606), a second flow channel (607) and a third flow channel (603 f) are respectively arranged in the auxiliary liquid inlet chip bodies (600), a first through hole which can be communicated with the first flow channel (606), a second through hole which can be communicated with the second flow channel (607) and a third through hole which can be communicated with the third flow channel (608) are sequentially arranged on the first pressing valve body (400) from bottom to top, the first liquid outlet control module (603) comprises a first upper control liquid outlet flow channel (603 a), a second upper control liquid outlet flow channel (603 d) and a third upper control liquid outlet flow channel (603 f) which are arranged in the auxiliary liquid inlet chip bodies (600) and are arranged between the first control holes and the rotating valve body (800), the first liquid outlet control flow channel (603 c) which can be communicated with the first end (603 a) of the first liquid outlet control flow channel (603 d) which can be far away from the first end of the first end (603 a) which can be communicated with the first end (603) of the first liquid inlet chip bodies (607), one end of the third through hole far away from the third flow channel (608) can be communicated with one end of the third upper control liquid outlet flow channel (603 f), and the first upper control liquid outlet flow channel (603 a), the second upper control liquid outlet flow channel (603 d) and the third upper control liquid outlet flow channel (603 f) can be respectively communicated with the corresponding first liquid inlet flow channel (702) through the rotary valve body (800); the upper end of the first lower control liquid outlet channel (603 i) is communicated with the first upper control liquid outlet channel (603 a), the first upper control liquid outlet channel (603 a) is communicated with an auxiliary liquid inlet channel (600) at the first lower control liquid outlet channel (603 i), a first upper liquid outlet control valve (603 b) and a first lower liquid outlet control valve (603 h) are respectively arranged in the first upper control liquid outlet channel (603 a) and the auxiliary liquid inlet channel (600) at the first lower control liquid outlet channel, the upper end of the second middle control liquid outlet channel (603 c) is communicated with a second upper control liquid outlet channel (603 d), the upper end of the third middle control liquid outlet channel is communicated with a third upper control liquid outlet channel (603 f), the second lower control liquid outlet channel (603 j), a third middle control liquid outlet channel (603 j) and a third middle control liquid outlet channel (603 c) are arranged in the liquid mixing chip body (700), the upper end of the second middle control liquid outlet channel (603 j) is communicated with the second middle control liquid outlet channel (603 c), the other end of the second middle control liquid outlet channel (603 c) can be respectively communicated with the third middle control liquid outlet channel (702) through a rotary valve body (800) and the second middle control liquid outlet channel (702) respectively, a second upper liquid outlet control valve (603 e) is arranged in the auxiliary liquid inlet chip body (600) at the second upper control liquid outlet channel (603 d) and the second middle control liquid outlet channel (603 c), a second middle liquid outlet control valve and a second lower liquid outlet control valve are respectively arranged in the liquid mixing chip body (700) at the second middle control liquid outlet channel (603 c) and the second lower control liquid outlet channel (603 j), a third upper liquid outlet control valve (603 g) and a third middle liquid outlet control valve are respectively arranged in the auxiliary liquid inlet chip body (600) at the third upper control liquid outlet channel (603 f) and the third middle control liquid outlet channel, and a third lower liquid outlet control valve is arranged in the liquid mixing chip body (700) at the third lower control liquid outlet channel;
The reaction chip comprises a reaction chip body (200), wherein one upward end of the reaction chip body (200) is provided with a reaction module corresponding to a reaction liquid outlet groove (705), the reaction module comprises a plurality of reaction tanks (201) arranged on the reaction chip body (200), the reaction liquid outlet groove (705) covers the corresponding reaction module, and the reaction chip body (200) is arranged at the lower side of a liquid mixing chip body (700);
the waste liquid recovery chip body (100), waste liquid recovery chip body (100) one end up is equipped with waste liquid pond (101), waste liquid pond (101) set up the play liquid end in reaction play cistern (705), waste liquid recovery chip body (100) are in the downside of reaction chip body (200).
2. The multi-functional microfluidic chip according to claim 1, wherein: the liquid mixing chip body (700) close to one end of the rotary valve body (800) is internally provided with a plurality of second liquid inlet flow channels (704) corresponding to the second liquid storage modules (605), the number of the second liquid inlet flow channels (704) is the same as that of the second liquid storage tanks (605 a) in the group of second liquid storage modules (605), the liquid mixing chip body (700) is rotatably connected with a second motor valve body (900) corresponding to the second liquid storage modules (605), the second motor valve body (900) is also provided with a plurality of liquid inlet holes (903) corresponding to the second liquid inlet flow channels (704) one by one, the liquid mixing chip body (700) close to the rotary valve body (800) is internally provided with a plurality of second liquid inlet flow channels (704) corresponding to the second liquid storage tanks (605 a), and when the liquid inlet holes (903) on the second motor valve body (900) cover the corresponding second liquid inlet flow channels (704), the liquid outlet (901) on the second motor valve body (900) cover the liquid inlet ends of the liquid outlet tank (705).
3. The multifunctional microfluidic chip according to claim 1 or 2, wherein: the auxiliary liquid inlet chip body (600) is provided with a second control hole corresponding to the second liquid storage module (605), the auxiliary liquid inlet chip body (600) is connected with a second pressing valve body (500) capable of lifting through the second control hole, and different second liquid storage tanks (605 a) and second liquid outlet control modules (609) are communicated through lifting the second pressing valve body (500).
4. The multi-functional microfluidic chip according to claim 3, wherein: the second pressing valve body (500) and the first pressing valve body (400) have the same structure, and the first liquid outlet control module (603) and the second liquid outlet control module (609) have the same structure.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4036590A (en) * | 1975-06-03 | 1977-07-19 | Siemens Aktiengesellschaft | Method and apparatus for the automatic analysis of the concentration of an individual component of a fluid in a metal-depositing bath having several components |
| CN113025478A (en) * | 2021-02-25 | 2021-06-25 | 王奔 | Micro-fluidic device, detection system and detection method for POCT quantitative nucleic acid detection |
| CN113122613A (en) * | 2021-04-09 | 2021-07-16 | 四川微康朴澜医疗科技有限责任公司 | Totally enclosed fluorescence quantitative PCR micro-fluidic detection chip |
| CN113751090A (en) * | 2021-09-27 | 2021-12-07 | 扬州大学 | Portable full-automatic microfluidic device and method for detecting fresh water quality by using same |
| CN113769803A (en) * | 2021-09-30 | 2021-12-10 | 扬州大学 | Micro-fluidic chip for military detection of infection marker and detection method thereof |
-
2022
- 2022-03-09 CN CN202210221239.6A patent/CN114798018B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4036590A (en) * | 1975-06-03 | 1977-07-19 | Siemens Aktiengesellschaft | Method and apparatus for the automatic analysis of the concentration of an individual component of a fluid in a metal-depositing bath having several components |
| CN113025478A (en) * | 2021-02-25 | 2021-06-25 | 王奔 | Micro-fluidic device, detection system and detection method for POCT quantitative nucleic acid detection |
| CN113122613A (en) * | 2021-04-09 | 2021-07-16 | 四川微康朴澜医疗科技有限责任公司 | Totally enclosed fluorescence quantitative PCR micro-fluidic detection chip |
| CN113751090A (en) * | 2021-09-27 | 2021-12-07 | 扬州大学 | Portable full-automatic microfluidic device and method for detecting fresh water quality by using same |
| CN113769803A (en) * | 2021-09-30 | 2021-12-10 | 扬州大学 | Micro-fluidic chip for military detection of infection marker and detection method thereof |
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