CN114367144A - In-situ multi-channel water enrichment, filtration and fixation device and method - Google Patents
In-situ multi-channel water enrichment, filtration and fixation device and method Download PDFInfo
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- CN114367144A CN114367144A CN202210083218.2A CN202210083218A CN114367144A CN 114367144 A CN114367144 A CN 114367144A CN 202210083218 A CN202210083218 A CN 202210083218A CN 114367144 A CN114367144 A CN 114367144A
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- 238000001914 filtration Methods 0.000 title claims abstract description 112
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 86
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 17
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 121
- 238000005070 sampling Methods 0.000 claims abstract description 12
- 239000012528 membrane Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 82
- 239000012535 impurity Substances 0.000 claims description 21
- 238000004140 cleaning Methods 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 238000004891 communication Methods 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 7
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 2
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 238000011160 research Methods 0.000 description 4
- 239000000834 fixative Substances 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D36/00—Filter circuits or combinations of filters with other separating devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D35/00—Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
- B01D35/02—Filters adapted for location in special places, e.g. pipe-lines, pumps, stop-cocks
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N2001/1031—Sampling from special places
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
Abstract
The invention belongs to the field of deep sea in-situ water enrichment, filtration and fixation, and particularly relates to an in-situ multi-channel water enrichment, filtration and fixation device and a method. The invention meets the enrichment and filtration requirements of microorganisms in the water body, and completes high-flux filtration and injection of various base solutions of the water body by using a peristaltic pump multi-pump head mode and a multi-channel rotating valve body; the invention has the advantages of multiple sampling channels, good fixing effect, flexible and stable work, capability of quickly and effectively obtaining the filtering membrane sample and carrying out in-situ fixing, and the connectivity and the sealing performance of the filtering membrane sample can be finished by using a single valve body, thereby reducing a large number of external structures and additional valve bodies and having great practicability.
Description
Technical Field
The invention belongs to the field of deep sea in-situ water body enrichment, filtration and fixation, and particularly relates to an in-situ multi-channel water body enrichment, filtration and fixation device and method.
Background
The high-throughput, rapid and accurate enrichment and filtration of water body filtration samples is the primary condition for developing the research on marine water body microorganisms, so that the research and development of related equipment are developed by domestic main marine research institutions. The existing underwater equipment is divided into direct taking type water body equipment, in-situ enrichment and filtration type sampling equipment, in-situ enrichment and fixation type equipment and the like, and the existing underwater equipment has various types, such as a fidelity pressure maintaining sampler, a deep sea microorganism uncovered culture cabin, a deep sea microorganism in-situ enrichment device, an eight-channel deep sea in-situ microorganism culture device, a grid plate type microorganism culture device, a large-volume water sample suction filtration and sampling system of American Mclean company, a deep sea hydrothermal nozzle microorganism filtration and sampling device, a multistage membrane filtration pressure maintaining sampler, an in-situ microorganism filtration and fixation device developed by the deep sea science and engineering research institute of Chinese academy of sciences, a water body suspended sand in-situ time-sharing sampling and filtration system (CN 113069807A) and the like.
In summary, the enrichment filter system is more, but the deep water area multi-channel enrichment filter and fixing system is less, the multi-channel valve and the fixing liquid are injected, the multi-channel electromagnetic valve is used for completing multi-channel fluid on-off, the fixing liquid is injected by using an additional electric pump, but the work is completed by using the same pump and a rotating valve, which is not found yet.
Disclosure of Invention
Aiming at the characteristics of a peristaltic pump and a rotary valve body, the invention aims to provide an in-situ multi-channel water body enrichment, filtration and fixing device and method.
The purpose of the invention is realized by the following technical scheme:
the structure of the enrichment, filtration and fixation device comprises a peristaltic pump, a multi-channel rotating valve and an enrichment and filtration assembly, wherein the peristaltic pump is provided with at least two peristaltic pump channels, the inlet of one peristaltic pump channel is connected with an impurity filter through an external input channel, the inlet of the other peristaltic pump channel is connected with a fixed liquid, an input channel is arranged between the multi-channel rotating valve and the peristaltic pump, one end of the input channel is communicated with the input port of the rotating valve of the multi-channel rotating valve, branch channels with the same number as the number of the peristaltic pump channels are divided from the other end of the input channel, and each branch channel is connected with the outlet of one peristaltic pump channel; the multichannel rotary valve is provided with a plurality of rotary valve output ports, each rotary valve output port rotates along with the multichannel rotary valve, one end of each rotary valve output port is respectively communicated with the rotary valve input port in the rotating process, the other end of each rotary valve output port is respectively connected with a filtering channel, and each filtering channel is provided with an enrichment filtering component; the enrichment filtering component comprises a filtering channel, a filter and a cache box, one end of the filtering channel is connected with a rotary valve output port on the multi-channel rotary valve, and the other end of the filtering channel is sequentially connected with the filter and the cache box.
Wherein: the peristaltic pump and the multi-channel rotating valve are respectively connected with a control and power supply system, and the control and power supply system respectively controls and supplies power to the peristaltic pump and the multi-channel rotating valve to complete forward and reverse conversion of the multi-channel rotating valve and forward and reverse movement of the peristaltic pump.
Each branch channel is a parallel channel, and each branch channel is provided with a one-way valve which can only feed liquid to the multi-channel rotating valve.
Every enrichment filtering component still has vertical little water gauge and check valve, the feed liquor end of buffer case links to each other with the play liquid end of filter, the play liquid end of buffer case connects gradually vertical little water gauge, check valve.
The other structure of the enrichment, filtration and fixation device comprises a peristaltic pump, a multi-channel rotary valve, a two-position three-way valve and an enrichment and filtration assembly, wherein an output channel is arranged between the multi-channel rotary valve and the peristaltic pump, one end of the output channel is communicated with a rotary valve output port of the multi-channel rotary valve, the other end of the output channel is communicated with a peristaltic pump inlet of the peristaltic pump, the multi-channel rotary valve is provided with a plurality of rotary valve input ports, each rotary valve input port rotates along with the multi-channel rotary valve, one end of each rotary valve input port is respectively communicated with the rotary valve output port in the rotation process, the other end of one rotary valve input port is connected with a fixed liquid, the other end of the other rotary valve input ports is respectively connected with a filtration channel, and each filtration channel is provided with the enrichment and filtration assembly; the enrichment filtering assembly comprises an impurity filter, a filter input channel, a buffer tank, a filter, a tee joint and a filter output channel, wherein one end of the filter output channel is connected with the other end of the input port of the rotary valve, the other end of the filter output channel is connected with one interface of the tee joint, the second interface of the tee joint is sequentially connected with the filter and the buffer tank, the impurity filter is connected with the buffer tank through the filter input channel, and the third interface of the tee joint is connected with a drainage pipeline; the peristaltic pump of peristaltic pump export and the two-position three-way valve entry linkage of two-position three-way valve, the two-position three-way valve normally open export of two-position three-way valve has connect the outlet through the pipeline, the two-position three-way valve normally closed export of two-position three-way valve links to each other with the feed liquor end that the multichannel connects, the multichannel connects have the joint the same with enrichment filter assembly quantity, and every connects all to the filter input channel in an enrichment filter assembly through the stationary liquid passageway.
The peristaltic pump, the multi-channel rotating valve and the two-position three-way valve are respectively connected with a control and power supply system, and the control and power supply system respectively controls and supplies power to the peristaltic pump, the multi-channel rotating valve and the two-position three-way valve to complete forward and reverse conversion of the multi-channel rotating valve, forward and reverse movement of the peristaltic pump and conversion of a normally open outlet and a normally closed outlet of the two-position three-way valve.
The filter output channel between an interface of the tee joint and the input port of the rotary valve is provided with a vertical micro water meter, the filter input channel between the impurity filter and the cache box is provided with a one-way valve which can only feed liquid into the cache box, and the fixed liquid channel is connected between the one-way valve and the cache box.
The multi-channel rotary valve is characterized in that a plurality of rotary valve output ports are uniformly arranged on a multi-channel valve core of the multi-channel rotary valve along the circumferential direction or a plurality of rotary valve input ports are uniformly arranged on the multi-channel valve core along the circumferential direction, the multi-channel rotary valve is provided with one rotary valve input port or one rotary valve output port, the multi-channel valve core is connected with the output end of an underwater motor through an underwater coupler, the underwater motor drives the multi-channel valve core to rotate and perform conversion of each channel on the multi-channel rotary valve, a multi-channel rotary valve communication port of the multi-channel rotary valve is connected with a control and power supply system through a multi-channel rotary valve control cable, and the multi-channel rotary valve communication port is a control and power supply port of the underwater motor.
The invention relates to an in-situ multi-channel water body enrichment, filtration and fixation method, which adopts the in-situ multi-channel water body enrichment, filtration and fixation device of any one of claims 1 to 4 and 8, and comprises the following steps:
firstly, integrally disassembling and cleaning a shore-based end; the whole device is disassembled and disassembled, and then is cleaned;
step two, cleaning and assembling, and filling a stationary liquid in an initial state;
thirdly, lowering the device to the required water depth by using a cable on the ship, and carrying out enrichment filtration and fixing work; the multi-channel rotating valve is used for communicating an input port of the rotating valve with an output port of the rotating valve, a peristaltic pump channel connected with the impurity filter on the peristaltic pump is opened, so that external water sequentially passes through the impurity filter, the external input channel, the peristaltic pump, a branch channel, the input channel and the multi-channel rotating valve to enter a filtering channel, then passes through the filter and the buffer tank on the filtering channel to be diffused, and when flowing through the filter, the external water is enriched and filtered onto the filter;
reversely opening another peristaltic pump channel connected with the stationary liquid on the peristaltic pump to enable the stationary liquid to sequentially pass through the peristaltic pump, another branch channel, the input channel and the multi-channel rotating valve to enter the same filtering channel, and then to diffuse after passing through the filter and the buffer tank on the filtering channel, wherein when the stationary liquid flows through the filter, the stationary liquid is filtered onto the filter, and the buffer tank realizes micro-storage of the stationary liquid;
step five, the multichannel rotating valve communicates the input port of the rotating valve with the output port of the next rotating valve to realize the filtration of other filtration channels, and the step three and the step four are repeated;
step six, sampling and film taking work at the shore base end; and recovering the filter to the shore base end, disassembling each filter, and taking out the filter membrane to complete the whole operation.
The invention also discloses an in-situ multi-channel water body enrichment, filtration and fixation method, which adopts the in-situ multi-channel water body enrichment, filtration and fixation device as claimed in any one of claims 5 to 8, and comprises the following steps:
firstly, integrally disassembling and cleaning a shore-based end; the whole device is disassembled and disassembled, and then is cleaned;
step two, cleaning and assembling, and filling a stationary liquid in an initial state;
thirdly, lowering the device to the required water depth by using a cable on the ship, and carrying out enrichment filtration and fixing work; the multi-channel rotating valve is used for communicating a rotating valve input port connected with an enrichment filtering component with a rotating valve output port, starting the peristaltic pump, opening a two-position three-way valve normally open outlet of the two-position three-way valve, enabling the enrichment filtering component connected with the rotating valve input port communicated with the rotating valve output port to work, enabling external water to be sequentially discharged through an impurity filter, a filter input channel, a buffer tank, a filter, a tee joint, a filter output channel, a multi-channel rotating valve, an output channel, the peristaltic pump, the two-position three-way valve normally open outlet and a water outlet, or/and the external water is discharged through a three-way connector and a water discharge pipeline after passing through the filter, and enabling the external water to be enriched and filtered onto the filter when flowing through the filter;
step four, the multichannel rotating valve communicates a rotating valve input port connected with the fixed liquid with a rotating valve output port, the peristaltic pump is started, a two-position three-way valve normally-closed outlet of the two-position three-way valve is opened, the fixed liquid is discharged through a three-way third interface and a drainage pipeline after sequentially passing through the multichannel rotating valve, the output channel, the peristaltic pump, the two-position three-way valve normally-closed outlet, a multichannel connector, the fixed liquid channel, a buffer tank and the filter for enriching the external water body, the fixed liquid is filtered onto the filter when flowing through the filter for enriching the external water body, and the buffer tank realizes trace storage of the fixed liquid;
step five, the multi-channel rotating valve communicates the output port of the rotating valve with the input port of the next rotating valve to realize the filtration of other filtration channels, and the step three and the step four are repeated;
step six, sampling and film taking work at the shore base end; and recovering the filter to the shore base end, disassembling each filter, and taking out the filter membrane to complete the whole operation.
The invention has the advantages and positive effects that:
1. the invention has the advantages of multiple sampling channels, good fixing effect, flexible and stable work, and can quickly and effectively obtain the filter membrane sample and carry out in-situ fixing.
2. The invention has small and compact structure and simple design, the connectivity of the invention is completed by using the one-way valve body, and a large number of external structures and additional valve bodies are reduced.
Drawings
FIG. 1 is a schematic structural diagram of a first embodiment of an enrichment filtration and fixation device of the present invention;
FIG. 2 is a schematic view of the multi-channel rotary valve of FIG. 1
FIG. 3 is a schematic structural view of a second embodiment of the enrichment filtration and fixation device of the present invention;
wherein: 1 is a one-way valve A, 2 is a vertical micro water meter, 3 is a buffer box, 4 is a filter, 5 is a filtering channel, 6 is a rotary valve output port, 7 is a rotary valve input port, 8 is an input channel, 9 is a one-way valve B, 10 is a one-way valve C, 11 is an external input channel, 12 is a peristaltic pump channel A, 13 is a peristaltic pump channel B, 14 is a peristaltic pump, 15 is a stationary liquid, 16 is a multi-channel rotary valve, 17 is a peristaltic pump control cable, 18 is a multi-channel rotary valve control cable, 19 is a control and power supply system, 20 is a peristaltic pump channel A outlet, 21 is a peristaltic pump channel A inlet, 22 is a peristaltic pump channel B inlet, 23 is a peristaltic pump channel B outlet, 24 is an impurity filter, 25 is a multi-channel rotary valve communication port, 26 is an underwater motor, 27 is an underwater coupler, 28 is a multi-channel valve core, 29 is a filter input channel, 30 is a one-way valve D, 31 is a three-way valve, 32 is a filter output channel, 33 is an output channel, 34 is an inlet of a peristaltic pump, 35 is an outlet of the peristaltic pump, 36 is a multi-channel joint, 37 is a fixed liquid channel, 38 is a two-position three-way valve control cable, 39 is an inlet of a two-position three-way valve, 40 is a normally closed outlet of the two-position three-way valve, 41 is a normally open outlet of the two-position three-way valve, 42 is a two-position three-way valve, and 43 is a water outlet.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example one
As shown in fig. 1 and fig. 2, the enrichment, filtration and fixation device of the present embodiment includes a peristaltic pump 14, a multi-channel rotary valve 16, an enrichment and filtration assembly and a control and power supply system 19, the peristaltic pump 14 has at least two peristaltic pump channels, wherein an inlet of one of the peristaltic pump channels is connected to an impurity filter 24 through an external input channel 11, an inlet of the other peristaltic pump channel is connected to a stationary liquid 15, if there are more peristaltic pump channels, the peristaltic pump channels can be used for communicating other injectable water bodies, an input channel 8 is disposed between the multi-channel rotary valve 16 and the peristaltic pump 14, one end of the input channel 8 is communicated with a rotary valve 7 of the multi-channel rotary valve 16, the other end of the input channel 8 is divided into branch channels having the same number as the number of the peristaltic pump channels, and each branch channel is connected to an outlet of one of the peristaltic pump channel; each branch channel is a parallel channel, each branch channel is provided with a one-way valve which can only feed liquid to the multi-channel rotating valve 16, and the flow-through conversion of each one-way valve is utilized. The embodiment is provided with two peristaltic pump channels, namely a peristaltic pump channel A12 and a peristaltic pump channel B13, wherein an inlet 21 of the peristaltic pump channel A12 of the peristaltic pump channel A is connected with an impurity filter 24 through an external input channel 11, an inlet 22 of the peristaltic pump channel B13 is connected with stationary liquid 15, and the other end of the input channel 8 is divided into two branch channels which are respectively connected with an outlet 20 of the peristaltic pump channel A12 and an outlet 23 of the peristaltic pump channel B13 of the peristaltic pump channel A12; a branch channel of the outlet 23 of the peristaltic pump channel B and the input channel 8 is provided with a check valve B9 which can only feed liquid to the multi-channel rotating valve 16, and a branch channel of the outlet 20 of the peristaltic pump channel A and the input channel 8 is provided with a check valve C10 which can only feed liquid to the multi-channel rotating valve 16.
The multichannel rotary valve 16 is provided with a plurality of rotary valve output ports 6, each rotary valve output port 6 rotates along with the multichannel rotary valve 16, one end of each rotary valve output port 6 is respectively communicated with the rotary valve input port 7 in the rotating process, the other end of each rotary valve output port 6 is respectively connected with a filtering channel 5, and each filtering channel 5 is provided with an enrichment filtering component.
The enrichment filter assembly comprises a filter channel 5, a filter 4, a buffer tank 3, a vertical micro water meter 2 and a one-way valve A1, wherein one end of the filter channel 5 is connected with a rotary valve output port 6 on a multi-channel rotary valve 16, the other end of the filter channel is connected with a liquid inlet end of the filter 4, a liquid outlet end of the filter 4 is connected with a liquid inlet end of the buffer tank 3, and a liquid outlet end of the buffer tank 3 is sequentially connected with the vertical micro water meter 2 and the one-way valve A1; the buffer tank is used for filtering and buffering the stationary liquid, then the stationary liquid is diffused through the one-way valve A1, the stationary liquid in the buffer tank 3 is diffused first, and the stationary liquid in the filter 4 lasts for a longer time. The vertical micro water meter 2 can measure the flow of the water filtered by the filter 4 in each filtering channel 5.
A plurality of rotary valve output ports 6 are uniformly arranged on a multi-channel valve core 28 of the multi-channel rotary valve 16 of the embodiment along the circumferential direction, the multi-channel rotary valve 16 is provided with a rotary valve input port 7, the multi-channel valve core 28 is connected with an output end of an underwater motor 26 through an underwater coupler 27, the underwater motor 26 drives the multi-channel valve core 28 to rotate to convert each channel on the multi-channel rotary valve 16, a multi-channel rotary valve communication port 25 of the multi-channel rotary valve 16 is connected with a control and power supply system 19 through a multi-channel rotary valve control cable 18, and the multi-channel rotary valve communication port 25 is a control and power supply port of the underwater motor 26. The peristaltic pump 14 is connected with a control and power supply system 19 through a peristaltic pump control cable 17, and the control and power supply system 19 controls and supplies power to the peristaltic pump 14 and the multi-channel rotating valve 16 respectively to complete forward and reverse conversion of the multi-channel rotating valve 16 and forward and reverse movement of the peristaltic pump 14.
In the embodiment, the opening pressure of each check valve is 0.1MPa, and the reverse pressure is 1 MPa; all the lines of the filter channel 5 are manufactured by saint-gobain, france; the filter 4 is a stainless steel flat filter (142mm or 90mm) of MILLIPORE; the fixative solution is RNAlater liquid. The control and power supply system 19 of the present embodiment is prior art and will not be described herein.
The in-situ multi-channel water body enrichment, filtration and fixation method comprises the following steps:
firstly, integrally disassembling and cleaning a shore-based end; the whole device is disassembled and disassembled, and then is cleaned;
step two, assembling after cleaning, and filling fixing liquid 15 into RNAlater liquid in an initial state;
thirdly, lowering the device to the required water depth by using a cable on the ship, and carrying out enrichment filtration and fixing work; the control and power supply system 19 controls the multi-channel rotary valve 16 to communicate the rotary valve input port 7 with one rotary valve output port 6, and opens a peristaltic pump channel A12 on the peristaltic pump 14, so that external water sequentially passes through the impurity filter 24, the external input channel 11, a peristaltic pump channel A inlet 21, a peristaltic pump channel A outlet 20, a one-way valve C10, the input channel 8, the rotary valve input port 7 and the rotary valve output port 6 and then enters one filtering channel 5, and then is diffused after passing through the filter 4, the buffer tank 3, the vertical type micro-water meter 2 and the one-way valve A1 on the filtering channel 5, and when flowing through the filter 4, the external water is enriched and filtered onto the filter 4;
step four, reversely opening a peristaltic pump channel B13 connected with the stationary liquid 15 on the peristaltic pump 14 to enable the stationary liquid 15 to enter the same filtering channel 5 after sequentially passing through an inlet 22 of the peristaltic pump channel B, an outlet 23 of the peristaltic pump channel B, a one-way valve B9, an input channel 8, an input port 7 of a rotary valve and an output port 6 of the rotary valve, and then to diffuse after passing through a filter 4, a buffer tank 3, a vertical type micro-water meter 2 and a one-way valve A1 on the filtering channel 5, when the stationary liquid flows through the filter 4, the stationary liquid 15 is filtered onto the filter 4, and the buffer tank 3 realizes micro-storage of the stationary liquid 15; that is, the stationary liquid passing through the filter 4 enters the buffer tank 3, and the stationary liquid in the buffer tank 3 is diffused first when being diffused outward, so that the stationary liquid in the filter 4 lasts for a longer time;
step five, the control and power supply system 19 controls the multi-channel rotary valve 16 to communicate the input port 7 of the rotary valve with the output port 6 of the next rotary valve, so that the other filtering channels 5 are filtered, and the step three and the step four are repeated;
step six, sampling and film taking work at the shore base end; and (4) recovering to the shore base end, detaching each filter 4, and taking out the filter membrane to complete the whole operation.
Example two
As shown in fig. 2 and fig. 3, the enrichment filtering and fixing device of the present embodiment includes a peristaltic pump 14, a multi-channel rotary valve 16, a two-position three-way valve 42, an enrichment filtering component and a control and power supply system 19, an output channel 33 is disposed between the multi-channel rotary valve 16 and the peristaltic pump 14, one end of the output channel 33 is communicated with a rotary valve output port 6 of the multi-channel rotary valve 16, the other end of the output channel 33 is communicated with a peristaltic pump inlet 34 of the peristaltic pump 14, the multi-channel rotary valve 16 has a plurality of rotary valve input ports 7, each rotary valve input port 7 rotates with the multi-channel rotary valve 16, one end of each rotary valve input port 7 is respectively communicated with the rotary valve output port 6 during the rotation process, the other end of one of the rotary valve input ports 7 is connected with the fixed liquid 15, the other ends of the other rotary valve input ports 7 are respectively connected with a filtering channel, and each filtering channel is provided with an enrichment filtering component.
The enrichment filtering assembly of the embodiment comprises an impurity filter 24, a one-way valve A1, a filter input channel 29, a buffer tank 3, a filter 4, a tee joint 31, a vertical micro-water meter 2, a filter output channel 32 and a one-way valve D30, wherein one end of the filter output channel 32 is connected with the other end of a rotary valve input port 7, the other end of the filter output channel 32 is connected with one interface of the tee joint 31, the second interface of the tee joint 31 is connected with a liquid outlet end of the filter 4, a liquid inlet end of the filter 4 is connected with a liquid outlet end of the buffer tank 3, the impurity filter 24 is connected with a liquid inlet end of the buffer tank 3 through the filter input channel 29, and the filter input channel 29 is provided with the one-way valve A1 which can only feed liquid into the buffer tank 3; the third interface of the tee joint 31 is connected with a drainage pipeline, and the drainage pipeline is provided with a one-way valve D30 which can only drain water to the outside.
The peristaltic pump outlet 35 of the peristaltic pump 14 of this embodiment is connected to the two-position three-way valve inlet 39 of the two-position three-way valve 42, the two-position three-way valve normally-open outlet 41 of the two-position three-way valve 42 is connected to the water outlet 43 through a pipeline, the two-position three-way valve normally-closed outlet 40 of the two-position three-way valve 42 is connected to the liquid inlet of the multi-channel joint 36, the multi-channel joint 36 has joints with the same number as the enrichment filter assemblies, and each joint is connected to the filter input channel 29 of one enrichment filter assembly through the fixed liquid channel 37 and is located between the one-way valve a1 and the buffer tank 3.
A plurality of rotary valve input ports 7 are uniformly arranged on a multi-channel valve core 28 of the multi-channel rotary valve 16 of the embodiment along the circumferential direction, the multi-channel rotary valve 16 is provided with a rotary valve output port 6, the multi-channel valve core 28 is connected with an output end of an underwater motor 26 through an underwater coupler 27, the underwater motor 26 drives the multi-channel valve core 28 to rotate to convert each channel on the multi-channel rotary valve 16, a multi-channel rotary valve communication port 25 of the multi-channel rotary valve 16 is connected with a control and power supply system 19 through a multi-channel rotary valve control cable 18, and the multi-channel rotary valve communication port 25 is a control and power supply port of the underwater motor 26. The peristaltic pump 14 is connected with the control and power supply system 19 through a peristaltic pump control cable 17, the two-position three-way valve 42 is connected with the control and power supply system 19 through a two-position three-way valve control cable 38, and the control and power supply system 19 respectively controls and supplies power to the peristaltic pump 14, the multi-channel rotating valve 16 and the two-position three-way valve 42 to complete forward and reverse conversion of the multi-channel rotating valve 16, forward and reverse movement of the peristaltic pump 14 and conversion of a normally open outlet and a normally closed outlet of the two-position three-way valve 42.
In the embodiment, the opening pressure of each check valve is 0.1MPa, and the reverse pressure is 1 MPa; all the lines of the filter channel 5 are manufactured by saint-gobain, france; the filter 4 is a stainless steel flat filter (142mm or 90mm) of MILLIPORE; the fixative solution is RNAlater liquid. The control and power supply system 19 of the present embodiment is prior art and will not be described herein.
The in-situ multi-channel water body enrichment, filtration and fixation method comprises the following steps:
firstly, integrally disassembling and cleaning a shore-based end; the whole device is disassembled and disassembled, and then is cleaned;
step two, assembling after cleaning, and filling fixing liquid 15 into RNAlater liquid in an initial state;
thirdly, lowering the device to the required water depth by using a cable on the ship, and carrying out enrichment filtration and fixing work; the control and power supply system 19 controls the multi-channel rotating valve 16 to communicate the rotating valve input port 7 connected with the enrichment filter assembly with the rotating valve output port 6, the peristaltic pump 14 is started, the two-position three-way valve normally open outlet 41 of the two-position three-way valve 42 is opened, the enrichment filter assembly connected with the rotating valve input port 7 communicated with the rotating valve output port 6 works, the external water sequentially passes through the impurity filter 24, the one-way valve A1, the filter input channel 29, the buffer tank 3, the filter 4, the three-way valve 31, the filter output channel 32, the rotating valve input port 7, the rotating valve output port 6, the output channel 33, the peristaltic pump inlet 34, the peristaltic pump outlet 35, the two-position three-way valve inlet 39, the two-position three-way valve normally open outlet 41 and the water outlet 43 to be discharged, or/and the external water are discharged from the water discharge pipeline through the one-way valve D by the third interface of the three-way valve 31 after passing through the filter 4, when the water flows through the filter 4, the external water is enriched and filtered onto the filter 4;
step four, the multi-channel rotating valve 16 communicates a rotating valve input port 7 connected with the fixed liquid 15 with a rotating valve output port 6, the peristaltic pump 14 is started, a two-position three-way valve normally-closed outlet 40 of a two-position three-way valve 42 is opened, the fixed liquid 15 sequentially passes through the rotating valve input port 7, the rotating valve output port 6, an output channel 33, a peristaltic pump inlet 34, a peristaltic pump outlet 35, a two-position three-way valve inlet 39, a two-position three-way valve normally-closed outlet 40, a multi-channel joint 36, a fixed liquid channel 37, a cache box 3 and the filter 4 for enriching the external water body, then is discharged through a three-way port 31 and a one-way valve D30 through a drainage pipeline, when flowing through the filter 4 for enriching the external water body, the fixed liquid 15 is filtered onto the filter 4, and the cache box 3 realizes micro-storage of the fixed liquid 15;
step five, the control and power supply system 19 controls the multi-channel rotary valve 16 to communicate the output port 6 of the rotary valve with the input port 7 of the next rotary valve, so that the filtration of other filtration channels is realized, and the step three and the step four are repeated;
step six, sampling and film taking work at the shore base end; and (4) recovering to the shore base end, detaching each filter 4, and taking out the filter membrane to complete the whole operation.
The invention has small and compact structure, strong corrosion resistance and more enrichment and filtration channels, and can be widely applied to ocean deep sea water samples needing strict fixation. The invention meets the requirements of high-flux filtration and fixation of deep-sea water, meets the acquisition of original data of DNA and RNA, and can be used for deep-sea microbial research.
Claims (10)
1. The utility model provides an in situ multichannel water enrichment is filtered and fixing device which characterized in that: the device comprises a peristaltic pump (14), a multi-channel rotary valve (16) and an enrichment filter assembly, wherein the peristaltic pump (14) is provided with at least two peristaltic pump channels, the inlet of one peristaltic pump channel is connected with an impurity filter (24) through an external input channel (11), the inlet of the other peristaltic pump channel is connected with a fixed liquid (15), an input channel (8) is arranged between the multi-channel rotary valve (16) and the peristaltic pump (14), one end of the input channel (8) is communicated with a rotary valve input port (7) of the multi-channel rotary valve (16), branch channels with the same number as the peristaltic pump channels are divided from the other end of the input channel (8), and each branch channel is connected with the outlet of one peristaltic pump channel; the multichannel rotary valve (16) is provided with a plurality of rotary valve output ports (6), each rotary valve output port (6) rotates along with the multichannel rotary valve (16), one end of each rotary valve output port (6) is respectively communicated with the rotary valve input port (7) in the rotating process, the other end of each rotary valve output port (6) is respectively connected with a filtering channel (5), and each filtering channel (5) is provided with an enrichment filtering component; the enrichment filtering component comprises a filtering channel (5), a filter (4) and a buffer box (3), one end of the filtering channel (5) is connected with a rotating valve output port (6) on a multi-channel rotating valve (16), and the other end of the filtering channel is sequentially connected with the filter (4) and the buffer box (3).
2. The in situ multi-channel water body enrichment, filtration and fixation device of claim 1, wherein: the peristaltic pump (14) and the multi-channel rotating valve (16) are respectively connected with a control and power supply system (19), and the control and power supply system (19) respectively controls and supplies power to the peristaltic pump (14) and the multi-channel rotating valve (16) to complete forward and reverse conversion of the multi-channel rotating valve (16) and forward and reverse movement of the peristaltic pump (14).
3. The in situ multi-channel water body enrichment, filtration and fixation device of claim 1, wherein: each branch channel is a parallel channel, and each branch channel is provided with a one-way valve which can only feed liquid to the multi-channel rotating valve (16).
4. The in situ multi-channel water body enrichment, filtration and fixation device of claim 1, wherein: every enrichment filtering component still has upright micro water meter (2) and check valve, the feed liquor end of buffer case (3) links to each other with the play liquid end of filter (4), the play liquid end of buffer case (3) connects gradually upright micro water meter (2), check valve.
5. The utility model provides an in situ multichannel water enrichment is filtered and fixing device which characterized in that: the device comprises a peristaltic pump (14), a multi-channel rotary valve (16), a two-position three-way valve (42) and an enrichment filter assembly, wherein an output channel (33) is arranged between the multi-channel rotary valve (16) and the peristaltic pump (14), one end of the output channel (33) is communicated with a rotary valve output port (6) of the multi-channel rotary valve (16), the other end of the output channel (33) is communicated with a peristaltic pump inlet (34) of the peristaltic pump (14), the multi-channel rotary valve (16) is provided with a plurality of rotary valve input ports (7), each rotary valve input port (7) rotates along with the multi-channel rotary valve (16), one end of each rotary valve input port (7) is respectively communicated with the rotary valve output port (6) in the rotating process, the other end of one rotary valve input port (7) is connected with a fixed liquid (15), and the other ends of the other rotary valve input ports (7) are respectively connected with a filter channel, each filtering channel is provided with an enrichment filtering component; the enrichment filtering assembly comprises an impurity filter (24), a filter input channel (29), a buffer tank (3), a filter (4), a tee joint (31) and a filter output channel (32), one end of the filter output channel (32) is connected with the other end of the rotary valve input port (7), the other end of the filter output channel (32) is connected with one interface of the tee joint (31), the second interface of the tee joint (31) is sequentially connected with the filter (4) and the buffer tank (3), the impurity filter (24) is connected with the buffer tank (3) through the filter input channel (29), and the third interface of the tee joint (31) is connected with a drainage pipeline; the peristaltic pump of peristaltic pump (14) pump export (35) is connected with two-position three-way valve entry (39) of two-position three-way valve (42), two-position three-way valve normally open export (41) of two-position three-way valve (42) have connect outlet (43) through the pipeline, two-position three-way valve normally closed export (40) of two-position three-way valve (42) link to each other with the inlet end of multichannel joint (36), multichannel joint (36) have with enrichment filtering component quantity the same joint, every connects all to filter input channel (29) in an enrichment filtering component through fixed liquid passageway (37).
6. The in situ multi-channel water body enrichment, filtration and fixation device of claim 5, wherein: the peristaltic pump (14), the multi-channel rotary valve (16) and the two-position three-way valve (42) are respectively connected with the control and power supply system (19), and the control and power supply system (19) respectively controls and supplies power to the peristaltic pump (14), the multi-channel rotary valve (16) and the two-position three-way valve (42) to complete forward and reverse conversion of the multi-channel rotary valve (16), forward and reverse movement of the peristaltic pump (14) and conversion of a normally open outlet and a normally closed outlet of the two-position three-way valve (42).
7. The in situ multi-channel water body enrichment, filtration and fixation device of claim 5, wherein: be equipped with upright micro water meter (2) on filter output channel (32) between an interface of tee bend (31) and rotary valve input port (7), be equipped with the check valve that only can feed liquor to buffer tank (3) on filter input channel (29) between impurity filter (24) and buffer tank (3), stationary liquid passageway (37) connect to between check valve and buffer tank (3).
8. The in-situ multi-channel water body enrichment, filtration and fixation device of claim 1 or 5, wherein: a plurality of rotary valve output ports (6) are uniformly arranged on a multi-channel valve core (28) of the multi-channel rotary valve (16) along the circumferential direction or a plurality of rotary valve input ports (7) are uniformly arranged along the circumferential direction, the multichannel rotary valve (16) has a rotary valve inlet (7) or a rotary valve outlet (6), the multi-channel valve core (28) is connected with the output end of the underwater motor (26) through an underwater coupling (27), the underwater motor (26) drives the multi-channel valve core (28) to rotate to switch each channel on the multi-channel rotary valve (16), a multi-channel rotary valve communication port (25) of the multi-channel rotary valve (16) is connected with a control and power supply system (19) through a multi-channel rotary valve control cable (18), the multi-channel rotary valve communication port (25) is a control and power supply port of an underwater motor (26).
9. An in-situ multi-channel water enrichment, filtration and fixation method is characterized in that: the in-situ multi-channel water body enrichment, filtration and fixation device of any one of claims 1 to 4 and 8 is adopted, and comprises the following steps
Firstly, integrally disassembling and cleaning a shore-based end; the whole device is disassembled and disassembled, and then is cleaned;
step two, the assembly is carried out after cleaning, and in an initial state, a stationary liquid (15) is filled;
thirdly, lowering the device to the required water depth by using a cable on the ship, and carrying out enrichment filtration and fixing work; the multi-channel rotary valve (16) is used for communicating a rotary valve input port (7) with a rotary valve output port (6), a peristaltic pump channel connected with an impurity filter (24) on the peristaltic pump (14) is opened, so that external water sequentially passes through the impurity filter (24), the external input channel (11), the peristaltic pump, a branch channel, the input channel (8) and the multi-channel rotary valve (16) to enter a filtering channel (5), and then is diffused after passing through the filter (4) and the buffer tank (3) on the filtering channel (5), and when flowing through the filter (4), the external water is enriched and filtered onto the filter (4);
step four, reversely opening another peristaltic pump channel connected with the stationary liquid (15) on the peristaltic pump (14) to enable the stationary liquid (15) to enter the same filtering channel (5) through the peristaltic pump (14), another branch channel, the input channel (8) and the multi-channel rotating valve (16) in sequence, then to diffuse after passing through the filter (4) and the buffer tank (3) on the filtering channel (5), when flowing through the filter (4), the stationary liquid (15) is filtered onto the filter (4), and the buffer tank (3) realizes micro storage of the stationary liquid (15);
step five, the multi-channel rotary valve (16) communicates the rotary valve input port (7) with the next rotary valve output port (6) to realize the filtration of other filtration channels (5), and the step three and the step four are repeated;
step six, sampling and film taking work at the shore base end; and (4) recovering to the shore base end, disassembling each filter (4), and taking out the filter membrane to complete the whole operation.
10. An in-situ multi-channel water enrichment, filtration and fixation method is characterized in that: the in-situ multi-channel water body enrichment, filtration and fixation device of any one of claims 5 to 8 is adopted, and comprises the following steps
Firstly, integrally disassembling and cleaning a shore-based end; the whole device is disassembled and disassembled, and then is cleaned;
step two, the assembly is carried out after cleaning, and in an initial state, a stationary liquid (15) is filled;
thirdly, lowering the device to the required water depth by using a cable on the ship, and carrying out enrichment filtration and fixing work; the multi-channel rotating valve (16) is communicated with a rotating valve input port (7) and a rotating valve output port (6) which are connected with an enrichment filtering assembly, the peristaltic pump (14) is opened, a two-position three-way valve normally-open outlet (41) of a two-position three-way valve (42) is opened, the enrichment filtering assembly connected with the rotating valve input port (7) communicated with the rotating valve output port (6) works, and external water sequentially passes through an impurity filter (24), a filter input channel (29), a buffer storage box (3), a filter (4), a tee joint (31), a filter output channel (32), the multi-channel rotating valve (16), an output channel (33), the peristaltic pump (14), the two-position three-way valve normally-open outlet (41) and a water outlet (43) to be discharged, or/and the external water passes through a third interface, a third interface and a third interface, a tee joint (31) and a third interface, a filter (31) and a third interface, a filter (3) and a third interface, a filter output channel, a filter (31) and a third interface, a filter output channel, a filter, and a filter, a filter, The water is discharged through a drainage pipeline, and when the water flows through the filter (4), the outside water is enriched and filtered onto the filter (4);
fourthly, the multi-channel rotating valve (16) is used for communicating a rotating valve input port (7) connected with the fixed liquid (15) with a rotating valve output port (6), the peristaltic pump (14) is started, a two-position three-way valve normally-closed outlet (40) of the two-position three-way valve (42) is opened, the fixed liquid (15) sequentially passes through the multi-channel rotating valve (16), an output channel (33), the peristaltic pump (14), the two-position three-way valve normally-closed outlet (40), a multi-channel joint (36), a fixed liquid channel (37), a buffer tank (3) and the filter (4) for enriching the external water body and then is discharged through a drainage pipeline by a third interface of a tee joint (31), when flowing through the filter (4) for enriching the external water body, the fixed liquid (15) is filtered onto the filter (4), and the buffer tank (3) is used for realizing micro storage of the fixed liquid (15);
step five, the multi-channel rotating valve (16) enables an output port (6) of the rotating valve to be communicated with an input port (7) of the next rotating valve, so that the other filtering channels are filtered, and the step three and the step four are repeated;
step six, sampling and film taking work at the shore base end; and (4) recovering to the shore base end, disassembling each filter (4), and taking out the filter membrane to complete the whole operation.
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