CN114798018A - Multifunctional micro-fluidic chip - Google Patents
Multifunctional micro-fluidic chip Download PDFInfo
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- CN114798018A CN114798018A CN202210221239.6A CN202210221239A CN114798018A CN 114798018 A CN114798018 A CN 114798018A CN 202210221239 A CN202210221239 A CN 202210221239A CN 114798018 A CN114798018 A CN 114798018A
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- 239000007788 liquid Substances 0.000 claims abstract description 629
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 47
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 32
- 238000003825 pressing Methods 0.000 claims description 36
- 239000002699 waste material Substances 0.000 claims description 27
- 239000011550 stock solution Substances 0.000 claims description 26
- 238000011084 recovery Methods 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 26
- 239000000243 solution Substances 0.000 description 20
- 238000002347 injection Methods 0.000 description 7
- 239000007924 injection Substances 0.000 description 7
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- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention discloses a multifunctional microfluidic chip, which comprises a detection assembly, wherein the detection assembly comprises a rotary valve body and an auxiliary liquid inlet chip body, a liquid mixing chip body is fixed at the lower side of one end of the auxiliary liquid inlet chip body in the left and right directions, a first liquid storage module and at least one group of second liquid storage modules are distributed on the auxiliary liquid inlet chip body, the rotary valve body is rotatably connected to the auxiliary liquid inlet chip body and the liquid mixing chip body, a liquid mixing flow channel is arranged in the liquid mixing chip body, a reaction liquid outlet groove is formed in the downward end of the liquid mixing chip body, a first liquid outlet control module is arranged between the first liquid storage module and the rotary valve body, and the first liquid outlet control module and the rotary valve body jointly control the connection and disconnection of liquid flowing out of the first liquid storage module into the liquid mixing flow channel; the reaction chip body, the reaction module corresponding to reaction liquid outlet trough is set on the upward end of the reaction chip body; the invention can realize the detection of different markers according to the actual detection requirement.
Description
Technical Field
The invention relates to the technical field of nucleic acid detection, in particular to a multifunctional microfluidic chip.
Background
The microfluidic technology can integrate basic operation units such as sample preparation, reaction, separation and the like in a biochemical analysis process on one chip to automatically complete the analysis process, has the advantages of less sample consumption, high detection speed, simple and convenient operation and the like, and is often used for marker detection in the medical field to realize diagnosis of related diseases. The existing micro-fluidic chip comprises an upper chip body, wherein an intermediate chip body is fixed on the lower side of the upper chip body, a lower chip body is fixed on the lower side of the intermediate chip body, the intermediate chip body is often used for solution reaction, the lower chip body is often used for recovering waste liquid, the upper chip body realizes the storage of liquid and the liquid inlet according to the liquid inlet sequence, the micro-fluidic chip only has a detection system, a plurality of detection systems can not be established through the structure of a chip, a disease corresponds to a chip structure, and the application range is small.
Disclosure of Invention
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.
The present invention has been made in view of the above and/or other problems occurring in the prior art of microfluidics.
Therefore, the present invention is directed to a multifunctional microfluidic chip, which can implement multiple detection systems and has a wider application range.
In order to solve the technical problems, the invention provides the following technical scheme: a multifunctional micro-fluidic chip comprises a chip body,
detection subassembly, detection subassembly includes rotary valve body and supplementary feed liquor chip body, supplementary feed liquor chip body is fixed with muddy liquid chip body at the downside of left right direction one end, first stock solution module and at least a set of second stock solution module of having arranged on the supplementary feed liquor chip body, supplementary feed liquor chip body and muddy liquid chip body between have the rotation hole, rotary valve body rotationally connects on supplementary feed liquor chip body and muddy liquid chip body through rotating the hole, this internal liquid mixing flow way that is equipped with of muddy liquid chip, the one end that mixes liquid chip body down is equipped with the reaction drain tank, between first stock solution module and rotary valve body, between second stock solution module and rotary valve body supplementary feed liquor chip body and mixed chip body are originally internal to be equipped with first play liquid control module and second play liquid control module respectively, first play liquid control module and rotary valve body common control let in the through in the liquid mixing flow way from the liquid that first stock solution module flows out The second liquid outlet control module and the rotary valve body jointly control the on-off of the liquid flowing out of the second liquid storage module into the liquid inlet end of the reaction liquid outlet groove;
the reaction chip comprises a reaction chip body, wherein one upward end of the reaction chip body is provided with a reaction module corresponding to a reaction liquid outlet groove, the reaction module comprises a plurality of reaction tanks distributed on the reaction chip body, the reaction liquid outlet groove covers the corresponding reaction module, and the reaction chip body is arranged on the lower side of the liquid mixing chip body;
the waste liquid recovery chip body, waste liquid recovery chip body one end up is equipped with the waste liquid pond, the waste liquid pond sets up the play liquid end at reaction drain tank, the downside of waste liquid recovery chip body at reaction chip body.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: first stock solution module is including arranging at this internal a plurality of first stock solution ponds of supplementary feed liquor chip, near this internal first feed liquor runner of arranging with first stock solution pond quantity of the muddy liquid chip of rotary valve body one end, rotationally be connected with first motor valve body on the muddy liquid chip body, it has the liquid outlet that can cover muddy liquid runner one end to open on the first motor valve body, still arrange the feed liquor hole of a plurality of and first feed liquor runner one-to-ones on the first motor valve body, when the first feed liquor runner that the feed liquor hole cover corresponds, the liquid outlet just in time covers the one end that mixes the liquid runner.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the other end of the liquid mixing flow channel can be communicated with the liquid inlet end of the reaction liquid outlet groove.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the liquid mixing chip next to rotary valve body one end is this internal still to be equipped with a plurality of second feed liquor runners that correspond with second stock solution module, the quantity of the second stock solution in a plurality of second feed liquor runners and a set of second stock solution module is the same, rotationally be connected with the second motor valve body that corresponds with second stock solution module on mixing the liquid chip body, also arrange a plurality of feed liquor holes with second feed liquor runner one-to-one on the second motor valve body, near this internal second feed liquor runner that a plurality of and second stock solution tank quantity are the same of arranging of the liquid mixing chip of rotary valve body, during the second feed liquor runner that feed liquor hole cover on the second motor valve body corresponds, the liquid inlet end in the liquid outlet cover reaction liquid groove on the second motor valve body.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the first control hole that corresponds with first stock solution module of arranging on the supplementary feed liquor chip body, supplementary feed liquor chip body is connected with the first valve body of pressing that can go up and down through first control hole, through going up and down first press the valve body and make different first stock solution ponds communicate with first play liquid control module respectively.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the first liquid storage pool is provided with three first flow channels, a second flow channel and a third flow channel are respectively arranged in the auxiliary liquid inlet chip body between the three first liquid storage pools and the first control hole, a first through hole which can be communicated with the first flow channel, a second through hole which can be communicated with the second flow channel and a third through hole which can be communicated with the third flow channel are sequentially arranged on the first pressing valve body from bottom to top, the first liquid outlet control module comprises a first upper control liquid outlet flow channel, a second upper control liquid outlet flow channel and a third upper control liquid outlet flow channel which are arranged in the auxiliary liquid inlet chip body and between the first control hole and the rotary valve body, one end of the first through hole far away from the first flow channel can be communicated with one end of the first upper control liquid outlet flow channel, one end of the second through hole far away from the second flow channel can be communicated with one end of the second upper control liquid outlet flow channel, one end of the third through hole far away from the third flow channel can be communicated with one end of the third upper control liquid outlet flow channel, the first upper control liquid outlet flow channel, the second upper control liquid outlet flow channel and the third upper control liquid outlet flow channel are respectively communicated with the corresponding first liquid inlet flow channels through the rotary valve body.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the first liquid outlet control module further comprises a first lower control liquid outlet flow channel arranged in the auxiliary liquid inlet chip body, the upper end of the first lower control liquid outlet flow channel is communicated with the first upper control liquid outlet flow channel, and a first upper liquid outlet control valve and a first lower liquid outlet control valve are arranged in the auxiliary liquid inlet chip body at the first upper control liquid outlet flow channel and the first lower control liquid outlet flow channel respectively.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the first liquid outlet control module further comprises a second middle control liquid outlet flow channel and a third middle control liquid outlet flow channel which are arranged in the auxiliary liquid inlet chip body, the upper end of the second middle control liquid outlet flow channel is communicated with the second upper control liquid outlet flow channel, the upper end of the third middle control liquid outlet flow channel is communicated with the third upper control liquid outlet flow channel, a second lower control liquid outlet flow channel, a third middle control liquid outlet flow channel and a third lower control liquid outlet flow channel are arranged in the liquid mixing chip body, the upper end of the second lower control liquid outlet flow channel is communicated with the second middle control liquid outlet flow channel, the other ends of the second middle control liquid outlet flow channel and the second lower control liquid outlet flow channel can be communicated with the corresponding first liquid inlet flow channel through a rotary valve body, the upper end of the third lower control liquid outlet flow channel is communicated with the third middle control liquid outlet flow channel, and the other ends of the third middle control liquid outlet flow channel and the third lower control liquid outlet flow channel can be communicated with the corresponding first liquid inlet flow channel through a rotary valve body and the corresponding first liquid inlet flow channel The liquid flow channels are communicated, a second upper liquid outlet control valve is arranged in each of the auxiliary liquid inlet chips at the positions of the second upper control liquid outlet flow channel and the second middle control liquid outlet flow channel, a second middle liquid outlet control valve and a second lower liquid outlet control valve are arranged in each of the second middle control liquid outlet flow channel and the second lower control liquid outlet flow channel, a third upper liquid outlet control valve and a third middle liquid outlet control valve are arranged in each of the auxiliary liquid inlet chips at the positions of the third upper control liquid outlet flow channel and the third middle control liquid outlet flow channel, and a third lower liquid outlet control valve is arranged in each of the third lower control liquid outlet flow channel.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: open on the supplementary feed liquor chip body and have the second control hole that corresponds with second stock solution module, supplementary feed liquor chip body is connected with the second that can go up and down through the second control hole and presses the valve body, presses down the valve body through going up and down the second and makes different second stock solution pool and second play liquid control module intercommunication.
As a preferable scheme of the multifunctional microfluidic chip of the present invention, wherein: the second pressing valve body and the first pressing valve body are identical in structure, and the first liquid outlet control module and the second liquid outlet control module are identical in structure.
The invention has the beneficial effects that: according to the invention, through the arrangement of the second pressing valve body, the reagents in the first liquid storage tanks and the second liquid storage tanks are controlled to be discharged according to a required sequence; through the combined arrangement of the first liquid outlet control module, the second liquid outlet control module and the rotary valve body, the detection of different markers can be realized according to actual detection requirements, and the application range is wider.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise. Wherein:
fig. 1 is a perspective view of the present invention.
Fig. 2 is a three-dimensional structure diagram of the present invention when two ends of the first upper detection liquid outlet flow channel are respectively communicated with the first upper control liquid outlet flow channel and the liquid inlet flow channel, two ends of the second upper detection liquid outlet flow channel are respectively communicated with the second upper control liquid outlet flow channel and the liquid inlet flow channel, and two ends of the third upper detection liquid outlet flow channel are respectively communicated with the third upper control liquid outlet flow channel and the liquid inlet flow channel.
Fig. 3 is a partially enlarged view of a portion a in fig. 2.
Fig. 4 is a partially enlarged view of fig. 2 at B.
Fig. 5 is a partially enlarged view of C in fig. 2.
Fig. 6 is a three-dimensional structure diagram of the case where two ends of the first lower detection liquid outlet flow channel are respectively communicated with the first lower control liquid outlet flow channel and the liquid inlet flow channel, and two ends of the third middle detection liquid outlet flow channel are respectively communicated with the second middle control liquid outlet flow channel and the liquid inlet flow channel.
Fig. 7 is a partial enlarged view of fig. 6 at D.
Fig. 8 is a three-dimensional structure diagram of the case where two ends of the second middle detection liquid outlet flow channel are respectively communicated with the second middle control liquid outlet flow channel and the liquid inlet flow channel, and two ends of the third lower detection liquid outlet flow channel are respectively communicated with the third lower control liquid outlet flow channel and the liquid inlet flow channel.
Fig. 9 is a partial enlarged view at E in fig. 8.
Fig. 10 is a three-dimensional structure diagram of the second lower detection outlet flow channel of the present invention when two ends of the second lower detection outlet flow channel are respectively communicated with the second lower control outlet flow channel and the liquid inlet flow channel.
Fig. 11 is a partial enlarged view of fig. 10 at F.
Fig. 12 is a partial enlarged view at G in fig. 10.
FIG. 13 is a perspective view of the reaction chip body according to the present invention.
FIG. 14 is a perspective view of the waste liquid recovery chip body according to the present invention.
In the figure, 100 waste liquid recovery chip body, 101 waste liquid pool, 200 reaction chip body, 201 reaction pool, 202 waste liquid hole, 300 driving motor, 400 first pressing valve body, 500 second pressing valve body, 600 auxiliary liquid inlet chip body, 601 first injection port, 602 second injection port, 603 first liquid outlet control module, 603a first upper control liquid outlet flow channel, 603b first upper liquid outlet control valve, 603c second middle control liquid outlet flow channel, 603d second upper control liquid outlet flow channel, 603e second upper liquid outlet control valve, 603f third upper control liquid outlet flow channel, 603g third upper liquid outlet control valve, 603h first lower liquid outlet control valve, 603i first lower control liquid outlet flow channel, 603j second lower control liquid outlet flow channel, 604a first liquid storage pool, 605 second liquid storage pool, 605a second liquid storage pool, 606 first flow channel, 607 second flow channel 608, third flow channel, 609 a second liquid outlet control module, 700 a liquid mixing chip body, 701 a liquid mixing flow channel, 702 a first liquid inlet flow channel, 703 a second connecting flow channel, 704 a second liquid inlet flow channel, 705 a reaction liquid outlet groove, 706 a first connecting flow channel, 800 a rotary valve body, 801a first detection liquid outlet module, 801a-1 first upper detection liquid outlet flow channel, 801a-2 first lower detection liquid outlet flow channel, 801b third flow channel, 801b-1 third lower detection liquid outlet flow channel, 801b-2 third middle detection liquid outlet flow channel, 801b-3 third upper detection liquid outlet flow channel, 801c second flow channel, 801c-1 second upper detection liquid outlet flow channel, 801c-2 second middle detection liquid outlet flow channel, 801c-3 second lower detection liquid outlet flow channel, 802 a second detection liquid outlet module, 900 a second motor valve body, 901 liquid outlet, 902 control liquid hole, 903 liquid inlet hole, 904 negative pressure channel, 905 negative pressure connecting pipe, 906 rotation sink groove, 1000 first motor valve body.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1
Referring to fig. 1, 4, 5, 13 and 14, which are first embodiments of the present invention, this embodiment provides a multifunctional microfluidic chip, which has a simple and reliable structure and can improve the detection efficiency.
A multifunctional micro-fluidic chip comprises a detection assembly, the detection assembly comprises a rotary valve body 800 and an auxiliary liquid inlet chip body 600, a liquid mixing chip body 700 is fixed on the lower side of one end of the auxiliary liquid inlet chip body 600 in the left and right directions, a first liquid storage module 604 and at least one group of second liquid storage modules 605 are distributed on the auxiliary liquid inlet chip body 600, a rotating hole is formed between the auxiliary liquid inlet chip body 600 and the liquid mixing chip body 700, the rotary valve body 800 is rotatably connected to the auxiliary liquid inlet chip body 600 and the liquid mixing chip body 700 through the rotating hole, a liquid mixing flow channel 701 is arranged in the liquid mixing chip body 700, a reaction liquid outlet groove 705 is arranged at the downward end of the liquid mixing chip body 700, a first liquid outlet control module 603 and a second liquid outlet control module 609 are respectively arranged in the auxiliary liquid inlet chip body 600 and the liquid mixing chip body between the first liquid storage module 604 and the rotary valve body 800 and between the second liquid storage module 605 and the rotary valve body 800, the first liquid outlet control module 603 and the rotary valve body 800 jointly control the on-off of the liquid flowing out of the first liquid storage module 604 into the liquid mixing channel 701, and the second liquid outlet control module 609 and the rotary valve body 800 jointly control the on-off of the liquid flowing out of the second liquid storage module 605 into the liquid inlet end of the reaction liquid outlet groove 705;
the reaction chip body 200 is provided with a reaction module corresponding to the reaction liquid outlet tank 705, the reaction module comprises a plurality of reaction tanks 201 arranged on the reaction chip body 200, the reaction liquid outlet tank 705 covers the corresponding reaction module, the reaction chip body 200 at one end of the reaction module far away from the second motor valve body 900 is provided with a waste liquid hole 202, and the reaction chip body 200 is arranged at the lower side of the liquid mixing chip body 700;
waste liquid recovery chip body 100, waste liquid recovery chip body 100 one end up is equipped with waste liquid pond 101, and waste liquid pond 101 covers all waste liquid holes 202 positions, and waste liquid pond 101 sets up the play liquid end at reaction effluent groove 705, and waste liquid recovery chip body 100 is at the downside of reaction chip body 200.
Further, the first liquid storage module 604 comprises a plurality of first liquid storage tanks 604a arranged in the auxiliary liquid inlet chip body 600, first liquid inlet flow channels 702 with the same number as the first liquid storage tanks 604a are arranged in the liquid mixing chip body 700 close to one end of the rotary valve body 800, the liquid mixing chip body 700 is rotatably connected with a first motor valve body 1000, the first motor valve body 1000 is provided with a liquid outlet 901 capable of covering one end of the liquid mixing flow channel 701, the first motor valve body 1000 is also provided with a plurality of liquid inlet holes 903 corresponding to the first liquid inlet flow channels 702 one by one, when the corresponding first liquid inlet flow channels 702 are covered by the liquid inlet holes 903, the liquid outlet 901 just covers one end of the liquid mixing flow channel 701, and the other end of the liquid mixing flow channel 701 can be communicated with the liquid inlet end of the reaction liquid outlet tank 705.
Further, a plurality of second liquid inlet flow channels 704 corresponding to the second liquid storage modules 605 are further arranged in the liquid mixing chip body 700 close to one end of the rotary valve body 800, the number of the second liquid inlet flow channels 704 is the same as that of the second liquid storage pools 605a in one group of the second liquid storage modules 605, a second motor valve body 900 corresponding to the second liquid storage modules 605 is rotatably connected to the liquid mixing chip body 700, a plurality of liquid inlet holes 903 corresponding to the second liquid inlet flow channels 704 one to one are also arranged on the second motor valve body 900, a plurality of second liquid inlet flow channels 704 corresponding to the second liquid inlet flow channels 605a are arranged in the liquid mixing chip body 700 close to the rotary valve body 800, the number of the second liquid inlet flow channels 704 is the same as that of the second liquid storage pools 605a, and when the liquid inlet holes 903 on the second motor valve body 900 cover the corresponding second liquid inlet flow channels 704, the liquid outlet 901 on the second motor valve body 900 covers the liquid inlet end of the reaction liquid outlet tank 705.
Further, first control holes corresponding to the first liquid storage module 604 are distributed on the auxiliary liquid inlet chip body 600, the auxiliary liquid inlet chip body 600 is connected with a first lifting pressing valve body 400 through the first control holes, different first liquid storage pools 604a are respectively communicated with the first liquid outlet control module 603 through lifting the first pressing valve body 400, the number of the first liquid storage pools 604a is three, a first flow channel 606, a second flow channel 607 and a third flow channel 608 are respectively arranged in the auxiliary liquid inlet chip body 600 between the three first liquid storage pools 604a and the first control holes, a first through hole capable of being communicated with the first flow channel 606, a second through hole communicated with the second flow channel 607 and a third through hole capable of being communicated with the third flow channel 608 are sequentially arranged on the first pressing valve body 400 from bottom to top, the first liquid outlet control module 603 comprises a first upper control liquid outlet flow channel 603a, which is arranged in the auxiliary liquid inlet chip body 600 and is arranged between the first control hole and the rotary valve body 800, The second upper control liquid outlet flow channel 603d and the third upper control liquid outlet flow channel 603f, one end of the first through hole far away from the first flow channel 606 can be communicated with one end of the first upper control liquid outlet flow channel 603a, one end of the second through hole far away from the second flow channel 607 can be communicated with one end of the second upper control liquid outlet flow channel 603d, one end of the third through hole far away from the third flow channel 608 can be communicated with one end of the third upper control liquid outlet flow channel 603f, and the first upper control liquid outlet flow channel 603a, the second upper control liquid outlet flow channel 603d and the third upper control liquid outlet flow channel 603f can be respectively communicated with the corresponding first liquid inlet flow channels 702 through the rotary valve body 800.
Further, the first liquid outlet control module 603 further includes a first lower control liquid outlet flow channel 603i disposed in the auxiliary liquid inlet chip body 600, an upper end of the first lower control liquid outlet flow channel 603i is communicated with the first upper control liquid outlet flow channel 603a, and a first upper liquid outlet control valve 603b and a first lower liquid outlet control valve 603h are disposed in the auxiliary liquid inlet chip body 600 at the first upper control liquid outlet flow channel 603a and the first lower control liquid outlet flow channel 603i, respectively.
Further, the first liquid outlet control module 603 further comprises a second middle control liquid outlet flow channel 603c and a third middle control liquid outlet flow channel arranged in the auxiliary liquid inlet chip body 600, the upper end of the second middle control liquid outlet flow channel 603c is communicated with the second upper control liquid outlet flow channel 603d, the upper end of the third middle control liquid outlet flow channel is communicated with the third upper control liquid outlet flow channel 603f, a second lower control liquid outlet flow channel 603j, a third middle control liquid outlet flow channel and a third lower control liquid outlet flow channel are arranged in the liquid mixing chip body 700, the upper end of the second lower control liquid outlet flow channel 603j is communicated with the second middle control liquid outlet flow channel 603c, the other ends of the second middle control liquid outlet flow channel 603c and the second lower control liquid outlet flow channel 603j can be respectively communicated with the corresponding first liquid inlet flow channel 702 through the rotary valve body 800, and the upper end of the third lower control liquid outlet flow channel is communicated with the third middle control liquid outlet flow channel, the other ends of the third middle control liquid outlet flow passage and the third lower control liquid outlet flow passage can be respectively communicated with the corresponding first liquid inlet flow passage 702 through the rotary valve body 800, the auxiliary liquid inlet chip bodies 600 at the positions of the second upper control liquid outlet flow passage 603d and the second middle control liquid outlet flow passage 603c are respectively provided with a second upper liquid outlet control valve 603e, the liquid mixing chip bodies 700 at the positions of the second middle control liquid outlet flow passage 603c and the second lower control liquid outlet flow passage 603j are respectively provided with a second middle liquid outlet control valve and a second lower liquid outlet control valve, the auxiliary liquid inlet chip bodies 600 at the positions of the third upper control liquid outlet flow passage 603f and the third middle control liquid outlet flow passage are respectively provided with a third upper liquid outlet control valve 603g and a third middle liquid outlet control valve, and the liquid mixing chip body 700 at the position of the third lower control liquid outlet flow passage is provided with a third lower liquid outlet control valve.
Further, a second control hole corresponding to the second liquid storage module 605 is formed in the auxiliary liquid inlet chip body 600, the auxiliary liquid inlet chip body 600 is connected with a second pressing valve body 500 capable of lifting through the second control hole, the second pressing valve body 500 and the first pressing valve body 400 are identical in structure, the first liquid outlet control module 603 and the second liquid outlet control module 609 are identical in structure, and different second liquid storage tanks 605a and the second liquid outlet control module 609 are communicated by lifting the second pressing valve body 500.
Further, the other ends of the first upper control liquid outlet flow passage 603a, the first middle control liquid outlet flow passage, the second upper control liquid outlet flow passage 603d, the second middle control liquid outlet flow passage 603c, the second lower control liquid outlet flow passage 603j, the third upper control liquid outlet flow passage 603f, the third middle control liquid outlet flow passage and the third lower control liquid outlet flow passage are attached to the outer side of the rotary valve body 800.
The upward ends of the first motor valve body 1000 and the second motor valve body 900 are both provided with a rotary sinking groove 906, the auxiliary liquid inlet chip body 600 at the first liquid storage tank 604a is provided with a first injection port 601 through which an injection solution enters the first liquid storage tank 604a, and the auxiliary liquid inlet chip body 600 at the second liquid storage tank 605a is provided with a second injection port 602 through which an injection solution enters the second liquid storage tank 605 a; according to the liquid inlet sequence, the first pressing valve body 400 is pressed downwards, when the first through hole and the first flow channel 606 of the first pressing valve body 400 are communicated, the reagent corresponding to the first liquid storage tank 604a sequentially flows into the first upper control liquid outlet flow channel 603a and the first middle control liquid outlet flow channel in front of the first upper liquid outlet control valve 603b and the first lower middle control liquid outlet flow channel and the first lower control liquid outlet flow channel 603i in front of the first lower liquid outlet control valve 603h through the first flow channel 606 and the first through hole, the reagent flows into the rotary valve body 800 in a required sequence by controlling each liquid outlet control valve, and flows into the corresponding first liquid inlet flow channel 702 from the rotary valve body 800, the reagent flowing into the first liquid inlet flow channel 702 sequentially flows into the liquid mixing flow channel 701 through the liquid inlet hole 903 and the liquid outlet 901 of the first motor valve body 1000, when a proper amount of the detection reagent enters the liquid mixing flow channel 701, the first pressing valve body 400 is pressed, the first liquid storage tank 604a is isolated from the first control upper liquid outlet flow passage, if other detection reagents need to be mixed, the first pressing valve body 400 is continuously pressed, when the second through hole of the first pressing valve body 400 is communicated with the second flow passage 607, the reagents corresponding to the first liquid storage tank 604a sequentially flow into the second upper control liquid outlet flow passage 603d and the second middle control liquid outlet flow passage 603c in front of the second upper liquid outlet control valve 603e and the second lower middle control liquid outlet flow passage and the second lower control liquid outlet flow passage 603j in front of the second lower liquid outlet control valve through the second flow passage 607 and the second through hole, when a proper amount of detection reagents enter the mixed liquid flow passage 701, the first pressing valve body 400 is pressed to isolate the first liquid storage tank 604a from the second control upper liquid outlet flow passage, if other detection reagents need to be mixed, the first pressing valve body 400 is pressed, when the third through hole of the first pressing valve body 400 is communicated with the third flow passage 608, the reagent in the corresponding first liquid storage tank 604a flows into a third upper control liquid outlet flow channel 603f and a third upper middle control liquid outlet flow channel in front of a third upper liquid outlet control valve 603g, and a third lower middle control liquid outlet flow channel and a third lower control liquid outlet flow channel in front of a third lower liquid outlet control valve in sequence through a third flow channel 608 and a third through hole, when a proper amount of detection reagent enters a mixed liquid flow channel 701, a first pressing valve body 400 is pressed downwards to separate the third flow channel 608 from the third upper control liquid outlet flow channel, so that the detection reagents are mixed in the mixed liquid flow channel 701, and the reagent uniformly mixed by the mixed liquid flow channel 701 enters a reaction liquid outlet groove 705; storing a detection sample, a flushing solution or other detection solutions in different second liquid storage tanks 605a, and controlling each liquid outlet control valve in the second liquid outlet control module 609 according to the liquid outlet sequence to enable the solutions to enter the reaction liquid outlet tank 705 according to the set liquid outlet sequence to react with the uniformly mixed detection reagents; the utility model discloses according to actual need in first liquid storage tank 604a injection needs's detect reagent, through press down first press valve body 400 make the reagent that needs flow into control go out liquid runner 603a on first, control go out liquid runner 603d or control go out liquid runner 603f on the third on the second, realize three kinds of detect reagent or two kinds of detect reagent's mixture through controlling each play liquid control valve, press valve body 500 and correspond each setting of going out the liquid control valve through the second, realize the detection of different markers.
Example 2
Referring to fig. 12, this embodiment provides a multifunctional microfluidic chip capable of pumping a reagent in a mixed liquid channel 701 or a solution in a second liquid control module 609, which is a second embodiment of the present invention.
A multifunctional micro-fluidic chip is characterized in that a second motor valve body 900 is further provided with control liquid holes 902 communicated with a liquid outlet 901, an auxiliary liquid inlet chip body 600 is provided with two groups of second liquid storage modules 605 and two groups of second liquid outlet control modules 609 corresponding to the second liquid storage modules 605 in a one-to-one mode, a first connecting flow channel 706 and a second connecting flow channel 703 are arranged in a liquid mixing chip body 700 between the two second motor valve bodies 900, one end of the first connecting flow channel 706 can be communicated with the control liquid hole 902 on one second motor valve body 900, one end of the second connecting flow channel 703 can be communicated with the control liquid hole 902 of the other second motor valve body 900, and the other end of the first connecting flow channel 706 and the other end of the second connecting flow channel 703 are connected with the other end of the liquid mixing flow channel 701.
Further, a negative pressure connecting pipe 905 is arranged on the outer side of the second motor valve body 900 above the liquid mixing chip body 700, a negative pressure channel 904 is arranged on the second motor valve body 900, one end of the negative pressure channel 904 is communicated with the inner cavity of the negative pressure connecting pipe 905, and the other end of the negative pressure channel 904 is communicated with the liquid outlet 901.
When the device works, a negative pressure pump is connected to the negative pressure connecting pipe 905, the negative pressure pump pumps liquid in the liquid mixing channel 701 to the reaction liquid outlet tank 705 through the negative pressure connecting pipe 905 and the negative pressure channel 904, and after a proper amount of detection reagent uniformly mixed in the liquid mixing channel 701 enters the reaction liquid outlet tank 705, the first motor valve body 1000 is rotated, so that the liquid inlet hole 903 in the first motor valve body 1000 and the first liquid inlet channel 702 are staggered; in this embodiment, the negative pressure connection pipe 905 and the negative pressure channel 904 on the second motor valve 900 are arranged to pump the reagent toward the reaction liquid outlet tank 705, and the negative pressure pump can also act in the reverse direction to flow the liquid entering the reaction liquid outlet tank 705 toward the waste liquid tank 101.
Example 3
Referring to fig. 1 to 14, this embodiment provides a multifunctional microfluidic chip having multiple detection systems for performing multiple functional detections, according to a third embodiment of the present invention.
A multifunctional micro-fluidic chip further comprises a driving motor 300, the driving motor 300 is in transmission connection with a rotary valve body 800, wherein a first detection liquid outlet module 801 and two groups of second detection liquid outlet modules 802 which are in one-to-one correspondence with a second liquid outlet control module 609 are arranged in the rotary valve body 800, the first detection liquid outlet module 801 and the second detection liquid outlet modules 802 are identical in structure, the first detection liquid outlet module 801 comprises a first circulation assembly 801a, a second circulation assembly 801c and a third circulation assembly 801b which are arranged in the rotary valve body 800 and sequentially arranged from front to back at intervals at the axial position, the first circulation assembly 801a comprises a first upper detection liquid outlet flow channel 801a-1 and a second lower detection liquid outlet flow channel 801c-3 which are arranged at the same axial position, and the second circulation assembly 801c comprises a second upper detection liquid outlet flow channel 801c-1, a second lower detection liquid outlet flow channel 801c-3 which are arranged at the same axial position, A second middle detection liquid outlet flow channel 801c-2 and a second lower detection liquid outlet flow channel 801c-3, wherein the third circulation component 801b comprises a third upper detection liquid outlet flow channel 801b-3, a third middle detection liquid outlet flow channel 801b-2 and a third lower detection liquid outlet flow channel 801b-1 which are arranged at the same axial position, and the three first liquid inlet flow channels 702 are a first liquid inlet flow channel 702, a second first liquid inlet flow channel 702 and a third first liquid inlet flow channel 702 in sequence from front to back.
Further, when one end of the first upper detection liquid outlet flow channel 801a-1 covers the other end of the first upper control liquid outlet flow channel 603a, the other end of the first upper detection liquid outlet flow channel 801a-1 is communicated with one end of the first liquid inlet flow channel 702 away from the first motor valve body 1000, one end of the second upper detection liquid outlet flow channel 801c-1 covers the other end of the second upper control liquid outlet flow channel 603d, the other end of the second upper detection liquid outlet flow channel 801c-1 is communicated with one end of the second first liquid inlet flow channel 702 away from the first motor valve body 1000, one end of the third upper detection liquid outlet flow channel 801b-3 covers the other end of the third upper control liquid outlet flow channel 603f, and the other end of the third upper detection liquid outlet flow channel 801b-3 is communicated with one end of the third first liquid inlet flow channel 702 away from the first motor valve body 1000.
Further, when one end of the first lower detection liquid outlet flow channel 801a-2 covers the first intermediate control liquid outlet flow channel, the other end of the first lower detection liquid outlet flow channel 801a-2 is communicated with one end of the first liquid inlet flow channel 702 away from the first motor valve body 1000, one end of the third intermediate detection liquid outlet flow channel 801b-2 covers the third upper intermediate control liquid outlet flow channel, and the other end of the third intermediate detection liquid outlet flow channel 801b-2 is communicated with one end of the third first liquid inlet flow channel 702 away from the first motor valve body 1000.
Further, when one end of the second middle detection liquid outlet flow channel 801c-2 covers the other end of the second lower middle control liquid outlet flow channel, the other end of the second middle detection liquid outlet flow channel 801c-2 is communicated with one end of the second first liquid inlet flow channel 702 away from the first motor valve body 1000, one end of the third lower detection liquid outlet flow channel 801b-1 covers the other end of the third lower middle control liquid outlet flow channel, and the other end of the third lower detection liquid outlet flow channel 801b-1 is communicated with one end of the third first liquid inlet flow channel 702 away from the first motor valve body 1000.
Further, when one end of the second lower detection liquid outlet flow channel 801c-3 covers the other end of the second lower control liquid outlet flow channel 603j, the other end of the second lower detection liquid outlet flow channel 801c-3 is communicated with one end of the second first liquid inlet flow channel 702 away from the first motor valve body 1000.
The first lower detection effluent flow channel 801a-2, the third lower detection effluent flow channel 801b-1 or the second lower detection effluent flow channel 801c-3 of the second detection effluent module 802 contains detected molecules, such as fluorescent molecules, enzymes and the like; in the initial state, one end of the first upper detection effluent channel 801a-1, the second upper detection effluent channel 801c-1 and the third upper detection effluent channel 801b-3 on the rotary valve body 800 are aligned with the first upper control effluent channel 603a, the second upper control effluent channel 603d and the third upper control effluent channel 603f respectively, for example, when detecting nucleic acid, different detection reagents are stored in the three first reservoirs 604a respectively, a detection sample, a cleaning solution and magnetic beads are injected in the three second reservoirs 605a respectively, the negative pressure pump is connected with the second motor valve body 900 through the negative pressure connection tube 905, the first upper effluent control valve 603b, the second upper effluent control valve 603e and the third upper effluent control valve 603g are opened, the first pressing valve body 400 is pressed to communicate the first through hole with the first flow channel 606, the first upper effluent control valve 603b on the first upper control effluent channel 603a is opened, after a proper amount of detection reagent flows into the first upper control liquid outlet flow channel 603a, the first pressing valve body 400 is continuously pressed downwards to enable the second through hole to be communicated with the second flow channel 607, the detection reagent corresponding to the first liquid storage tank 604a flows into the second upper control liquid outlet flow channel 603d, then the first pressing valve body 400 is pressed downwards to enable the third through hole to be communicated with the third flow channel 608, the detection reagent corresponding to the first liquid storage tank 604a flows into the third upper control liquid outlet flow channel 603f, the negative pressure pump acts, the three detection reagents respectively flow into the mixed liquid flow channel 701 through the first upper detection liquid outlet flow channel 801a-1, the second upper detection liquid outlet flow channel 801c-1 and the third upper detection liquid outlet flow channel 801b-3 and are pumped into the reaction liquid outlet groove 705 after being mixed, the second pressing valve body 500 and the corresponding second upper liquid outlet control valve 603e are controlled to act to enable the solution in the second storage tank to be discharged according to the detection sequence, combining a negative pressure pump, pumping the corresponding solution into the reaction liquid outlet tank 705, introducing the solution entering the reaction liquid outlet tank 705 into the reaction tank 201, and introducing the redundant solution into the waste liquid tank 101 through the waste liquid hole 202; if other marker detection is needed, when detecting molecules of the inner clad of the second lower detection liquid outlet flow channel 801c-3 in the second detection liquid outlet module 802 need to be used, the driving motor 300 acts, the rotary valve body 800 rotates, one end of the second lower detection liquid outlet flow channel 801c-3 in the second detection liquid outlet module 802 is just aligned with the second lower control liquid outlet flow channel 603j, the driving motor 300 stops acting, at the moment, the other end of the second lower detection liquid outlet flow channel 801c-3 of the second detection liquid outlet module 802 is communicated with the second liquid inlet flow channel 704, the second pressing valve body 500 is pressed to the height corresponding to the second through hole and the second flow channel 607, the second lower liquid outlet control valve in the second control liquid outlet module is opened, the liquid inlet hole 903 of the second motor valve body 900 is staggered with the second liquid inlet flow channel 704, and the reaction solution in the second liquid storage pool 605a and the detecting molecules of the inner clad of the second lower detection liquid outlet flow channel 801c-3 in the second detection liquid outlet module 802 are inverted When a proper amount of reaction solution flows out of the second liquid storage tank 605a and enters the second lower detection liquid outlet channel 801c-3, the second pressing valve body 500 is controlled to move downwards, so that corresponding through holes in the second liquid storage tank 605a and the second pressing valve body 500 are staggered, and after the set reaction time is reached, the second motor valve body 900 is controlled to rotate, so that the liquid inlet hole 903 of the second motor valve body 900 is communicated with the second liquid inlet channel 704, the second motor valve body 900 stops rotating, the negative pressure pump acts, and the solution reacted with detection molecules is rapidly pumped into the reaction liquid outlet channel 705 by the negative pressure pump, so that the luminescence detection is conveniently realized; according to the invention, the rotary valve body 800 is rotated to a required position by controlling the action of the driving motor 300, so that a required detection liquid outlet flow channel is aligned with a corresponding control liquid outlet flow channel, and then the corresponding liquid outlet control valve is controlled to act, so that a reaction solution flows into the reaction liquid outlet groove 705 according to a liquid outlet sequence and reacts in the reaction tank 201, thereby realizing detection of different markers.
It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (10)
1. A multifunctional microfluidic chip is characterized in that: which comprises
Detection subassembly, detection subassembly includes rotary valve body (800) and supplementary feed liquor chip body (600), supplementary feed liquor chip body (600) is fixed with and mixes liquid chip body (700) at the downside of left right direction one end, first stock solution module (604) and at least a set of second stock solution module (605) have arranged on supplementary feed liquor chip body (600), supplementary feed liquor chip body (600) and mix have the rotation hole between liquid chip body (700), rotary valve body (800) rotationally connect on supplementary feed liquor chip body (600) and mix liquid chip body (700) through rotating the hole, be equipped with in mixing liquid chip body (700) and mix liquid runner (701), mix liquid chip body (700) one end down and be equipped with reaction and go out liquid tank (705), supplementary feed liquor chip body (600) between first stock solution module (604) and rotary valve body (800), second stock solution module (605) and rotary valve body (800) and mix chip body A first liquid outlet control module (603) and a second liquid outlet control module (609) are respectively arranged in the reaction liquid outlet groove (705), the first liquid outlet control module (603) and the rotary valve body (800) jointly control the on-off of the liquid flowing out of the first liquid storage module (604) and introduced into the liquid mixing flow channel (701), and the second liquid outlet control module (609) and the rotary valve body (800) jointly control the on-off of the liquid flowing out of the second liquid storage module (605) and introduced into the liquid inlet end of the reaction liquid outlet groove (705);
the reaction chip comprises a reaction chip body (200), wherein a reaction module corresponding to a reaction liquid outlet groove (705) is arranged at one upward end of the reaction chip body (200), the reaction module comprises a plurality of reaction pools (201) distributed on the reaction chip body (200), the reaction liquid outlet groove (705) covers the corresponding reaction module, and the reaction chip body (200) is arranged at the lower side of a liquid mixing chip body (700);
the waste liquid recovery chip comprises a waste liquid recovery chip body (100), wherein a waste liquid pool (101) is arranged at one upward end of the waste liquid recovery chip body (100), the waste liquid pool (101) is arranged at a liquid outlet end of a reaction liquid outlet groove (705), and the waste liquid recovery chip body (100) is arranged on the lower side of the reaction chip body (200).
2. The multifunctional microfluidic chip of claim 1, wherein: first stock solution module (604) are including arranging a plurality of first stock solutions (604 a) in supplementary feed liquor chip body (600), near arranging first feed liquor runner (702) the same with first stock solution (604 a) quantity in the muddy liquid chip body (700) of rotary valve body (800) one end, it rotationally is connected with first motor valve body (1000) on the muddy liquid chip body (700), it has liquid outlet (901) that can cover muddy liquid runner (701) one end to open on first motor valve body (1000), still arrange a plurality of and first feed liquor runner (702) one-to-one's feed liquor hole (903) on first motor valve body (1000), when feed liquor hole (903) cover corresponding first feed liquor runner (702), liquid outlet (901) just in time cover the one end of mixing liquid runner (701).
3. The multifunctional microfluidic chip of claim 2, wherein: the other end of the liquid mixing flow channel (701) can be communicated with the liquid inlet end of the reaction liquid outlet groove (705).
4. The multifunctional microfluidic chip of any one of claims 1 to 3, wherein: a plurality of second liquid inlet flow channels (704) corresponding to the second liquid storage modules (605) are further arranged in the liquid mixing chip body (700) close to one end of the rotary valve body (800), the number of the second liquid inlet flow channels (704) is the same as that of the second liquid storage tanks (605 a) in one group of the second liquid storage modules (605), a second motor valve body (900) corresponding to the second liquid storage modules (605) is rotatably connected to the liquid mixing chip body (700), a plurality of liquid inlet holes (903) corresponding to the second liquid inlet flow channels (704) in a one-to-one manner are also arranged on the second motor valve body (900), a plurality of second liquid inlet flow channels (704) with the same number as that of the second liquid storage tanks (605 a) are arranged in the liquid mixing chip body (700) close to the rotary valve body (800), and when the corresponding second liquid inlet flow channels (704) are covered by the liquid inlet holes (903) on the second motor valve body (900), a liquid outlet (901) on the second motor valve body (900) covers the liquid inlet end of the reaction liquid outlet tank (705).
5. The multifunctional microfluidic chip of any one of claims 1 to 3, wherein: the first control hole that corresponds with first stock solution module (604) is arranged in supplementary feed liquor chip body (600), supplementary feed liquor chip body (600) are connected with the first valve body (400) of pressing that can go up and down through first control hole, through going up and down first press valve body (400) make different first stock solution pond (604 a) communicate with first play liquid control module (603) respectively.
6. The multifunctional microfluidic chip of claim 4, wherein: the first liquid storage tank (604 a) is provided with three, a first flow channel (606), a second flow channel (607) and a third flow channel (608) are respectively arranged in an auxiliary liquid inlet chip body (600) between the three first liquid storage tanks (604 a) and a first control hole, a first through hole capable of being communicated with the first flow channel (606), a second through hole communicated with the second flow channel (607) and a third through hole capable of being communicated with the third flow channel (608) are sequentially arranged on the first pressing valve body (400) from bottom to top, the first liquid outlet control module (603) comprises a first upper control liquid outlet flow channel (603 a), a second upper control liquid outlet flow channel (603 d) and a third upper control liquid outlet flow channel (603 f) which are arranged in the auxiliary liquid inlet chip body (600) and between the first control hole and the rotary valve body (800), one end of the first through hole far away from the first flow channel (606) can be communicated with one end of the first upper control liquid outlet flow channel (603 a), one end, far away from the second flow channel (607), of the second through hole can be communicated with one end, far away from the third flow channel (608), of the second upper control liquid outlet flow channel (603 d), one end, far away from the third flow channel (608), of the third through hole can be communicated with one end, far away from the third flow channel, of the third upper control liquid outlet flow channel (603 f), and the first upper control liquid outlet flow channel (603 a), the second upper control liquid outlet flow channel (603 d) and the third upper control liquid outlet flow channel (603 f) can be respectively communicated with the corresponding first liquid inlet flow channel (702) through the rotary valve body (800).
7. The multifunctional microfluidic chip of claim 6, wherein: the first liquid outlet control module (603) further comprises a first lower control liquid outlet flow channel (603 i) arranged in the auxiliary liquid inlet chip body (600), the upper end of the first lower control liquid outlet flow channel (603 i) is communicated with the first upper control liquid outlet flow channel (603 a), and a first upper liquid outlet control valve (603 b) and a first lower liquid outlet control valve (603 h) are respectively arranged in the auxiliary liquid inlet chip body (600) at the positions of the first upper control liquid outlet flow channel (603 a) and the first lower control liquid outlet flow channel (603 i).
8. The multifunctional microfluidic chip of claim 7, wherein: the first liquid outlet control module (603) further comprises a second middle control liquid outlet flow channel (603 c) and a third middle control liquid outlet flow channel which are arranged in the auxiliary liquid inlet chip body (600), the upper end of the second middle control liquid outlet flow channel (603 c) is communicated with a second upper control liquid outlet flow channel (603 d), the upper end of the third middle control liquid outlet flow channel is communicated with a third upper control liquid outlet flow channel (603 f), a second lower control liquid outlet flow channel (603 j), a third middle control liquid outlet flow channel and a third lower control liquid outlet flow channel are arranged in the liquid mixing chip body (700), the upper end of the second lower control liquid outlet flow channel (603 j) is communicated with the second middle control liquid outlet flow channel (603 c), and the other ends of the second middle control liquid outlet flow channel (603 c) and the second lower control liquid outlet flow channel (603 j) can be respectively communicated with the corresponding first liquid inlet flow channel (702) through a rotary valve body (800), the upper end of the third lower control liquid outlet flow channel is communicated with a third middle control liquid outlet flow channel, the other ends of the third middle control liquid outlet flow channel and the third lower control liquid outlet flow channel can be respectively communicated with a corresponding first liquid inlet flow channel (702) through a rotary valve body (800), a second upper liquid outlet control valve (603 e) is arranged in each auxiliary liquid inlet chip body (600) at the positions of the second upper control liquid outlet flow channel (603 d) and the second middle control liquid outlet flow channel (603 c), a second middle liquid outlet control valve and a second lower liquid outlet control valve are respectively arranged in each liquid mixing chip body (700) at the positions of the second middle control liquid outlet flow channel (603 c) and the second lower control liquid outlet flow channel (603 j), a third upper liquid outlet control valve (603 g) and a third middle liquid outlet control valve are respectively arranged in each auxiliary liquid inlet chip body (600) at the positions of the third upper control liquid outlet flow channel (603 f) and the third middle control liquid outlet flow channel, a third lower liquid outlet control valve is arranged in the liquid mixing chip body (700) at the third lower control liquid outlet flow passage.
9. The multifunctional microfluidic chip of claim 6, wherein: the auxiliary liquid inlet chip body (600) is provided with a second control hole corresponding to the second liquid storage module (605), the auxiliary liquid inlet chip body (600) is connected with a second pressing valve body (500) capable of lifting through the second control hole, and different second liquid storage tanks (605 a) are communicated with the second liquid outlet control module (609) by lifting the second pressing valve body (500).
10. The multifunctional microfluidic chip of claim 7, wherein: the second pressing valve body (500) and the first pressing valve body (400) are identical in structure, and the first liquid outlet control module (603) and the second liquid outlet control module (609) are identical in structure.
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| CN114798018B (en) | 2023-05-12 |
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