CN114794116A - Pesticide composition containing pseudolaric acid B and application thereof in preventing and treating plant diseases - Google Patents
Pesticide composition containing pseudolaric acid B and application thereof in preventing and treating plant diseases Download PDFInfo
- Publication number
- CN114794116A CN114794116A CN202110070048.XA CN202110070048A CN114794116A CN 114794116 A CN114794116 A CN 114794116A CN 202110070048 A CN202110070048 A CN 202110070048A CN 114794116 A CN114794116 A CN 114794116A
- Authority
- CN
- China
- Prior art keywords
- essential oil
- pseudolaric acid
- active component
- pseudolaric
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- GOHMRMDXUXWCDQ-UHFFFAOYSA-N pseudolaric acid B Natural products CC(=O)OC12CCC(C)=CCC11C(=O)OC(C)(C=CC=C(C)C(O)=O)C2CC1 GOHMRMDXUXWCDQ-UHFFFAOYSA-N 0.000 title claims abstract description 59
- VDGOFNMYZYBUDT-YDRCMHEVSA-N pseudolaric acid b Chemical compound C([C@@]12OC(C)=O)CC(C(=O)OC)=CC[C@@]11C(=O)O[C@](C)(\C=C\C=C(/C)C(O)=O)[C@@H]2CC1 VDGOFNMYZYBUDT-YDRCMHEVSA-N 0.000 title claims abstract description 59
- VDGOFNMYZYBUDT-UHFFFAOYSA-N pseudolarix acid B Natural products CC(=O)OC12CCC(C(=O)OC)=CCC11C(=O)OC(C)(C=CC=C(C)C(O)=O)C2CC1 VDGOFNMYZYBUDT-UHFFFAOYSA-N 0.000 title claims abstract description 59
- 239000000203 mixture Substances 0.000 title claims abstract description 21
- 201000010099 disease Diseases 0.000 title claims abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 6
- 239000000575 pesticide Substances 0.000 title description 4
- 239000000341 volatile oil Substances 0.000 claims abstract description 46
- 241000196324 Embryophyta Species 0.000 claims abstract description 26
- 150000002989 phenols Chemical class 0.000 claims abstract description 19
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 10
- 241000123650 Botrytis cinerea Species 0.000 claims abstract description 9
- 241000221696 Sclerotinia sclerotiorum Species 0.000 claims abstract description 7
- 241000223195 Fusarium graminearum Species 0.000 claims abstract description 5
- 241000223221 Fusarium oxysporum Species 0.000 claims abstract description 4
- 241000813090 Rhizoctonia solani Species 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 3
- UILPJVPSNHJFIK-UHFFFAOYSA-N Paeonol Chemical compound COC1=CC=C(C(C)=O)C(O)=C1 UILPJVPSNHJFIK-UHFFFAOYSA-N 0.000 claims description 20
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 claims description 12
- YLTGFGDODHXMFB-UHFFFAOYSA-N isoacetovanillon Natural products COC1=CC=C(C(C)=O)C=C1O YLTGFGDODHXMFB-UHFFFAOYSA-N 0.000 claims description 10
- MLIBGOFSXXWRIY-UHFFFAOYSA-N paeonol Natural products COC1=CC=C(O)C(C(C)=O)=C1 MLIBGOFSXXWRIY-UHFFFAOYSA-N 0.000 claims description 10
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 claims description 10
- KJPRLNWUNMBNBZ-QPJJXVBHSA-N (E)-cinnamaldehyde Chemical compound O=C\C=C\C1=CC=CC=C1 KJPRLNWUNMBNBZ-QPJJXVBHSA-N 0.000 claims description 7
- 241000233616 Phytophthora capsici Species 0.000 claims description 7
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 claims description 6
- 244000223760 Cinnamomum zeylanicum Species 0.000 claims description 6
- 239000005770 Eugenol Substances 0.000 claims description 6
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 claims description 6
- RECUKUPTGUEGMW-UHFFFAOYSA-N carvacrol Chemical compound CC(C)C1=CC=C(C)C(O)=C1 RECUKUPTGUEGMW-UHFFFAOYSA-N 0.000 claims description 6
- HHTWOMMSBMNRKP-UHFFFAOYSA-N carvacrol Natural products CC(=C)C1=CC=C(C)C(O)=C1 HHTWOMMSBMNRKP-UHFFFAOYSA-N 0.000 claims description 6
- 235000007746 carvacrol Nutrition 0.000 claims description 6
- KJPRLNWUNMBNBZ-UHFFFAOYSA-N cinnamic aldehyde Natural products O=CC=CC1=CC=CC=C1 KJPRLNWUNMBNBZ-UHFFFAOYSA-N 0.000 claims description 6
- 229940117916 cinnamic aldehyde Drugs 0.000 claims description 6
- 235000017803 cinnamon Nutrition 0.000 claims description 6
- 229960002217 eugenol Drugs 0.000 claims description 6
- WYXXLXHHWYNKJF-UHFFFAOYSA-N isocarvacrol Natural products CC(C)C1=CC=C(O)C(C)=C1 WYXXLXHHWYNKJF-UHFFFAOYSA-N 0.000 claims description 6
- ZCCUUQDIBDJBTK-UHFFFAOYSA-N psoralen Chemical compound C1=C2OC(=O)C=CC2=CC2=C1OC=C2 ZCCUUQDIBDJBTK-UHFFFAOYSA-N 0.000 claims description 6
- LFYJSSARVMHQJB-UHFFFAOYSA-N Backuchiol Natural products CC(C)=CCCC(C)(C=C)C=CC1=CC=C(O)C=C1 LFYJSSARVMHQJB-UHFFFAOYSA-N 0.000 claims description 5
- 235000013628 Lantana involucrata Nutrition 0.000 claims description 5
- 235000006677 Monarda citriodora ssp. austromontana Nutrition 0.000 claims description 5
- 240000007673 Origanum vulgare Species 0.000 claims description 5
- 244000223014 Syzygium aromaticum Species 0.000 claims description 5
- 235000016639 Syzygium aromaticum Nutrition 0.000 claims description 5
- 239000005844 Thymol Substances 0.000 claims description 5
- 240000002657 Thymus vulgaris Species 0.000 claims description 5
- 235000007303 Thymus vulgaris Nutrition 0.000 claims description 5
- LFYJSSARVMHQJB-GOSISDBHSA-N bakuchinol Natural products CC(C)=CCC[C@@](C)(C=C)C=CC1=CC=C(O)C=C1 LFYJSSARVMHQJB-GOSISDBHSA-N 0.000 claims description 5
- LFYJSSARVMHQJB-QIXNEVBVSA-N bakuchiol Chemical compound CC(C)=CCC[C@@](C)(C=C)\C=C\C1=CC=C(O)C=C1 LFYJSSARVMHQJB-QIXNEVBVSA-N 0.000 claims description 5
- KXXXNMZPAJTCQY-UHFFFAOYSA-N bakuchiol Natural products CC(C)CCCC(C)(C=C)C=Cc1ccc(O)cc1 KXXXNMZPAJTCQY-UHFFFAOYSA-N 0.000 claims description 5
- 229940117895 bakuchiol Drugs 0.000 claims description 5
- 229960000790 thymol Drugs 0.000 claims description 5
- 239000001585 thymus vulgaris Substances 0.000 claims description 5
- 240000002505 Pogostemon cablin Species 0.000 claims description 4
- 235000011751 Pogostemon cablin Nutrition 0.000 claims description 4
- VXGRJERITKFWPL-UHFFFAOYSA-N 4',5'-Dihydropsoralen Natural products C1=C2OC(=O)C=CC2=CC2=C1OCC2 VXGRJERITKFWPL-UHFFFAOYSA-N 0.000 claims description 3
- 241001330975 Magnaporthe oryzae Species 0.000 claims 1
- 230000002265 prevention Effects 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 10
- 206010059866 Drug resistance Diseases 0.000 abstract description 5
- 240000007594 Oryza sativa Species 0.000 abstract description 5
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 5
- 235000009566 rice Nutrition 0.000 abstract description 5
- 229940079593 drug Drugs 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 238000002156 mixing Methods 0.000 description 20
- 230000002195 synergetic effect Effects 0.000 description 17
- 230000000694 effects Effects 0.000 description 13
- 230000001988 toxicity Effects 0.000 description 10
- 231100000419 toxicity Toxicity 0.000 description 10
- 235000013824 polyphenols Nutrition 0.000 description 7
- 241000233866 Fungi Species 0.000 description 5
- 244000052616 bacterial pathogen Species 0.000 description 5
- 239000003899 bactericide agent Substances 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 229920000742 Cotton Polymers 0.000 description 4
- 206010039509 Scab Diseases 0.000 description 4
- 244000061456 Solanum tuberosum Species 0.000 description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 description 4
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 239000004480 active ingredient Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 241000218682 Pseudolarix amabilis Species 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 244000000005 bacterial plant pathogen Species 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000013329 compounding Methods 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 240000002791 Brassica napus Species 0.000 description 2
- 235000006008 Brassica napus var napus Nutrition 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 208000003251 Pruritus Diseases 0.000 description 2
- 241000221662 Sclerotinia Species 0.000 description 2
- 208000002474 Tinea Diseases 0.000 description 2
- 241000130764 Tinea Species 0.000 description 2
- 230000000843 anti-fungal effect Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- -1 polyphenol compound Chemical class 0.000 description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- UUDZDKPKXAEKLA-UHFFFAOYSA-N 6'-O-Acetylpseudolaric acid B-O-D-glucopyranoside Natural products CC(=O)OC12CCC(C(=O)OC)=CCC1(C(O1)=O)CCC2C1(C)C=CC=C(C)C(=O)OC1OC(CO)C(O)C(O)C1O UUDZDKPKXAEKLA-UHFFFAOYSA-N 0.000 description 1
- 206010063409 Acarodermatitis Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001344131 Magnaporthe grisea Species 0.000 description 1
- 241001529744 Origanum Species 0.000 description 1
- 235000011203 Origanum Nutrition 0.000 description 1
- 241000218641 Pinaceae Species 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 235000011609 Pinus massoniana Nutrition 0.000 description 1
- 241000018650 Pinus massoniana Species 0.000 description 1
- WUENWZUJMIZJPA-UHFFFAOYSA-N Pseudolaric acid D Natural products CC1(CO)CCCC2(C)C3CCC4CC3(CCC12)C=C4C(O)=O WUENWZUJMIZJPA-UHFFFAOYSA-N 0.000 description 1
- 244000226566 Psoralea corylifolia Species 0.000 description 1
- 241000447727 Scabies Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- RBXVTEUAOTYIME-UHFFFAOYSA-N deacetylpseudolaric acid C2 Natural products OC12CCC(C(=O)OC)=CCC11C(=O)OC(C)(C=CC=C(C)C(O)=O)C2CC1 RBXVTEUAOTYIME-UHFFFAOYSA-N 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 229930004069 diterpene Natural products 0.000 description 1
- 150000004141 diterpene derivatives Chemical class 0.000 description 1
- YQNLDLVKFNZFTI-UHFFFAOYSA-N ent-isokaur-15(16)-en-17,19-dioic acid Natural products C1CC2(C=C3C(O)=O)CC3CCC2C2(C)C1C(C)(C(O)=O)CCC2 YQNLDLVKFNZFTI-UHFFFAOYSA-N 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 229930183946 pseudolaric acid Natural products 0.000 description 1
- GOHMRMDXUXWCDQ-MPVZDDSSSA-N pseudolaric acid a Chemical compound C([C@@]12OC(=O)C)CC(C)=CC[C@@]11C(=O)O[C@](C)(\C=C\C=C(/C)C(O)=O)[C@@H]2CC1 GOHMRMDXUXWCDQ-MPVZDDSSSA-N 0.000 description 1
- RBXVTEUAOTYIME-GPGKBOPFSA-N pseudolaric acid c1 Chemical compound C([C@@]12O)CC(C(=O)OC)=CC[C@@]11C(=O)O[C@](C)(\C=C\C=C(/C)C(O)=O)[C@@H]2CC1 RBXVTEUAOTYIME-GPGKBOPFSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 208000005687 scabies Diseases 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000011885 synergistic combination Substances 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a sterilization composition containing pseudolaric acid B, a micromolecular phenolic compound and plant essential oil. The mass part ratio of the pseudolaric acid B to the micromolecular phenolic compound to the plant essential oil is 1:1, 1:10, 1:30, 1:50 and 1: 100. The bactericidal composition can be used for preventing and treating rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, rice blast and fusarium oxysporum; the invention reduces the medication cost of the expensive pseudolaric acid B, is expected to delay or overcome the generation of drug resistance of diseases, prolongs the service life of the medicament and is beneficial to green treatment of the diseases.
Description
Technical Field
The invention relates to a pesticide composition, which is prepared from an active component A: pseudolaric acid B and active component B: thymol, carvacrol, eugenol, paeonol, bakuchiol, thyme essential oil, patchouli essential oil, clove essential oil, oregano essential oil, cinnamon essential oil and cinnamaldehyde are used as effective components, and the composition is suitable for preventing and treating plant diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, rice blast, fusarium oxysporum and phytophthora capsici.
Background
In recent years, the problem of drug resistance has attracted widespread attention in countries around the world, and the improper use and even abuse of antibiotics is one of the important factors contributing to drug resistance. The abuse of antibiotics is mainly shown in 3 aspects of clinical medication, unreasonable use of patients and animal husbandry, and the like, and the abuse problem in the animal husbandry is particularly serious and threatens the health of human beings. The introduction of food safety law in 2015 and 'bang kang order' in 2016 indicates that the search for and the development of novel, green, safe and efficient antibiotic substitutes is greatly promoted.
The golden larch bark (Pesudolaris cortex) is the dry root bark or near root bark of the Pinus massoniana (Pseudolarix amabilis (Nelson) Rehd.) belonging to the family Pinaceae. Has the effects of killing parasites, treating tinea and relieving itching, and can be widely used for treating scabies, tinea and pruritus. Pseudolaric Acid B (PAB) is a natural product with anticancer and antifungal activities extracted from the root bark of Pseudolarix amabilis. The effective antifungal component of cortex pseudolaricis is diterpene acid compound, and is a mixture. The composition also contains tannin, volatile oil, pseudolaric acid B, pseudolaric acid C, pseudolaric acid D, pseudolaric acid E, pseudolaric acid B-beta-D-glucoside and other components, and the content of pseudolaric acid is the highest.
The phenolic compounds refer to compounds containing phenolic hydroxyl groups in the structure, and comprise natural and chemically synthesized phenolic substances. Natural phenolic compounds are widely found in nature, most commonly in plants, and these compounds often contain multiple phenolic hydroxyl groups in their structure, also known as polyphenolic compounds. Phenolic hydroxyl in the molecular structure of the polyphenol compound is easy to generate substitution reaction and can phagocytose free radicals, so that the polyphenol compound has excellent antioxidant activity. In addition, the reported pharmacological activities of polyphenols also include anti-inflammatory, antibacterial, antiviral and antitumor effects. Phenolic compounds are widely present in plants in free and bound states, and phenolic compounds in different forms have similar biological activities, but exhibit different physiological activities. The comprehensive analysis of plant phenolic compounds is helpful for scientific development of potential synergistic effects of the plant phenolic compounds.
The plant essential oil is a secondary metabolite in the plant body and is a substance with recognized safety in the world. Many researches prove that the plant essential oil has various biological activities of resisting bacteria, diminishing inflammation, resisting oxidation, delaying aging and the like, and is widely applied to the fields of medical care, food industry, daily chemical products and the like. The origanum essential oil has been approved by the Ministry of agriculture of China as a drug feed additive capable of being added for a long time due to the characteristics of nature, no residue, antibiosis, difficulty in generating drug resistance and the like, thereby indicating that the natural plant extract is a great potential as an antibiotic substitute.
Many data show that plant essential oil can inhibit any microorganism according to the bacteriostatic mechanism of the plant essential oil, but the plant essential oil with different active ingredients has different microbial inhibition strength. Therefore, the synergistic effect of the plant essential oils can help to find out a compound green medicament which has no obvious influence on the growth of plants while inhibiting the growth of pathogenic bacteria, thereby achieving the effect of 'replacing resistance'.
A large number of experiments show that the pseudolaric acid B, the micromolecular phenolic compound and the plant essential oil are combined and applied, and have obvious synergistic effect on sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, rice blast, cotton wilt and phytophthora capsici. It can improve the control effect and reduce the dosage. At present, no report about the combined application of the pseudolaric acid B, small molecular phenolic compounds and plant essential oil exists.
Disclosure of Invention
The invention aims to provide a bactericide composition containing pseudolaric acid B, a micromolecular phenolic compound and plant essential oil, which is used for preventing and treating rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, rice blast, fusarium oxysporum and phytophthora capsici.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a bactericide composition containing pseudolaric acid B, a micromolecular phenolic compound and plant essential oil comprises an active component A and an active component B, wherein the active component A is pseudolaric acid B, the active component B is the micromolecular phenolic compound and the plant essential oil, and the mass part ratio of the active component A to the active component B is 1:1, 1:10, 1:30, 1:50 and 1: 100.
The bactericide composition provided by the invention has the following synergistic effects:
1) the sterilization composition provided by the invention improves the use effect of the medicament after compounding.
2) After compounding, the use amount of expensive pseudolaric acid B is also reduced.
3) The bactericidal composition provided by the invention has the advantages that the effective components with different action mechanisms are mixed, the control effect can be improved, the drug resistance of plant pathogenic bacteria is delayed, and the service life of the pesticide is prolonged.
Detailed Description
In order to better understand the essence of the present invention, the following examples are further illustrated, but the present invention is not limited to these examples. In these examples, all percentages are by mass unless otherwise indicated.
Example 1: a bactericide composition containing pseudolaric acid B, micromolecular phenolic compound and plant essential oil comprises an active component A and an active component B (shown as a structural formula 1), wherein the active component A is pseudolaric acid B, and the active component B is micromolecular phenolic compound and plant essential oil.
Active component B: thymol, carvacrol, eugenol, paeonol, bakuchiol, thyme essential oil, patchouli essential oil, clove essential oil, oregano essential oil, cinnamon essential oil, and cinnamaldehyde.
TABLE 1 antibacterial Activity of pseudolaric acid B and active ingredient B against 7 agricultural germs
Example 2: indoor activity determination of Sclerotinia sclerotiorum by mixing pseudolaric acid B and active component B
The plant pathogenic bacteria used in the experiment are strains preserved at 4 ℃ in a laboratory, and the adopted culture medium is a potato agar glucose culture medium (PDA for short). The PDA culture medium formula comprises: potato (peeled) 200g, glucose 20g, agar 15g, distilled water 1000mL, natural pH.
The PDA culture medium configuration method comprises the following steps: cleaning potato, peeling, weighing 200g, cutting into small pieces, boiling with distilled water for about 20min (the potato pieces are soft but not rotten), filtering with eight layers of gauze, adding distilled water to 1000mL of filtrate, adding 15g of agar and 20g of glucose, stirring to fully dissolve, subpackaging in triangular flasks, sterilizing at 121 ℃ for 20min, and cooling for later use.
The indoor activity is measured by a hypha growth rate method.
(1) Activating strains: and culturing the plant pathogenic bacteria on a PDA (personal digital assistant) plate at 25 ℃ for 3-6 days.
(2) Preparing a medicine board: heating and melting PDA culture medium, cooling to 45-50 deg.C, and adding the mixture with different concentrations to make into flat plate with medicine.
(3) Inoculating and culturing: in a super clean bench, a punch is used for beating a fungus cake (the diameter is 5mm) at the edge of hypha cultured for 3-6 days (the growth condition is as consistent as possible), then an inoculating needle is used for picking the fungus cake to the center of a medicine plate, and then the fungus cake is inversely cultured in an incubator (25 ℃).
(4) And (4) determining the result: after the blank control group is full of hyphae, the growth diameter of the hyphae of the administration group is measured by a cross method, and the inhibition rate is calculated.
(5) Inhibition (%) (control hypha diameter-treated hypha diameter)/(control hypha diameter-cake diameter) × 100, and 3 parallel experiments were performed for each concentration.
The compounding synergy of the bactericide is calculated according to the formula of Wadley (1967) to obtain the synergistic coefficient after mixing different medicaments:
in the formula, a and B represent pseudolaric acid B, and the ratio of two agents of micromolecular phenolic compound or plant essential oil B in the mixture, EC 50 (Exp) represents the theoretical EC for the mixture of A and B agents in a: B ratio 50 Value, EC 50 (Obs) actual observed EC after mixing of A and B agents 50 The value is obtained. SR>1.5 is synergistic effect; SR<0.5 is antagonistic; an SR between 0.5 and 1.5 indicates additive effect.
The indoor toxicity synergistic combination effect of the pseudolaric acid B and the active component B on the sclerotinia rot of colza is shown in Table 2.
TABLE 2 synergistic effect of mixing of pseudolaric acid B and active ingredient B on Sclerotinia sclerotiorum
As can be seen from Table 1, the mixing mass ratio of the pseudolaric acid B to the active component B is 1:1, the synergistic effect on sclerotinia rot of colza is obvious, wherein the SR value of the pseudolaric acid B and carvacrol is 3.15 at the mass ratio of 1: 1.
Example 3: indoor activity determination of mixing pseudolaric acid B and active component B on botrytis cinerea
The indoor toxicity synergism of the pseudolaric acid B and the active component B on Botrytis cinerea when mixed is shown in Table 3.
TABLE 3 synergistic effect of mixing pseudolaric acid B and active component B on indoor toxicity of Botrytis cinerea
As can be seen from Table 3, the mixing mass ratio of the pseudolaric acid B to the active component B is 1:1, 1:10, 1:30, 1:50, 1; the composition has obvious synergistic effect on botrytis cinerea at 100 hours, wherein the SR values of pseudolaric acid B, cinnamaldehyde, paeonol and bakuchiol are respectively as high as 13.02, 14.44 and 11.00 at a mass ratio of 1: 1; under the mass ratio of 1:10, SR values of pseudolaric acid B, oregano essential oil, cinnamyl aldehyde, paeonol and bakuchiol are respectively as high as 11.13, 10.82, 11.58 and 10.80; under the condition that the mass ratio of the pseudolaric acid B to the paeonol to the psoralea corylifolia is 1:30, the SR values are respectively as high as 5.32 and 3.26; the mass ratio of pseudolaric acid B to psoralen, paeonol, cinnamaldehyde and thyme essential oil is 1:50, and the SR values are respectively as high as 13.29, 6.57, 4.66 and 4.16; the mass ratio of the pseudolaric acid B to the psoralen to the paeonol is 1:100, and the SR values are respectively as high as 6.90 and 5.61.
Example 4: the indoor activity of the pseudolaric acid B and the active component B in the mixed mode on the wheat scab is determined, and the indoor toxicity synergism of the pseudolaric acid B and the active component B in the mixed mode on the wheat scab is shown in the table 4.
TABLE 4 synergistic effect of mixing pseudolaric acid B and active component B on indoor toxicity of wheat scab
As can be seen from Table 4, the pseudolaric acid B and the paeonol have obvious synergistic effect on the wheat scab germs at the mass ratio of 1:1, 1:10, 1:30, 1:50 and 1:100, and particularly have SR value as high as 15.04 at the mass ratio of 1: 30.
Example 5: the indoor activity of the pseudolaric acid B and the active component B in the blending of the pseudolaric acid B and the blast fungus is measured, and the indoor toxicity synergism of the pseudolaric acid B and the active component B in the blending of the pseudolaric acid B and the blast fungus is shown in Table 5.
TABLE 5 synergistic effect of mixing pseudolaric acid B and active component B on indoor toxicity of Magnaporthe grisea
As can be seen from Table 5, the mixing mass ratio of the pseudolaric acid B to the active component B is 1:1, 1:10, the synergistic effect on rice blast germs is obvious, and especially when the mass ratio of the pseudolaric acid B to the eugenol is 1:10, the SR value is as high as 3.04.
Example 6: indoor activity determination of cotton fusarium wilt by mixing pseudolaric acid B and active component B
The indoor toxicity synergism of the pseudolaric acid B and the active component B on cotton wilt pathogens by mixing is shown in Table 6.
TABLE 6 indoor toxicity synergism of Pseudolaric acid B and active ingredient B
As can be seen from Table 6, the mixing mass ratio of the pseudolaric acid B to the active component B is 1:1 and 1:10, the synergistic effect on cotton wilt germs is obvious, wherein the SR values of the pseudolaric acid B, the cinnamon essential oil and the clove essential oil are respectively 2.98 and 2.93 when the mass ratio of the pseudolaric acid B to the cinnamon essential oil is 1: 10.
Example 7: and (3) mixing pseudolaric acid B and the active component B to determine the indoor activity of phytophthora capsici.
TABLE 7 synergistic effect of mixing pseudolaric acid B and active component B on indoor toxicity of Phytophthora capsici
As can be seen from Table 7, the mixing mass ratio of the pseudolaric acid B to the active component B is 1:1, 1:10, the synergistic effect on phytophthora capsici is remarkable, and especially when the mixing mass ratio of the pseudolaric acid B to the thymol, the eugenol and the carvacrol is 1:1, the SR values are respectively as high as 4.93, 4.74 and 4.49.
Claims (3)
1. A sterilization composition containing pseudolaric acid B, micromolecular phenolic compounds and plant essential oil is characterized in that: the effective components include pseudolaric acid B, thymol, carvacrol, eugenol, paeonol, bakuchiol, thyme essential oil, patchouli essential oil, clove essential oil, oregano essential oil, cinnamon essential oil, and cinnamaldehyde. The mass part ratio of the pseudolaric acid B to the micromolecular phenolic compound to the plant essential oil is 1:1, 1:10, 1:30, 1:50 and 1: 100.
2. The composition of claim 1 having the following molecular structural features:
active component A: pseudolaric acid B
Active component B part structure
Active component B: thymol, carvacrol, eugenol, paeonol, psoralen, thyme essential oil, patchouli essential oil, clove essential oil, oregano essential oil, cinnamon essential oil, and cinnamaldehyde.
3. The use of the bactericidal composition containing pseudolaric acid B, a small molecular phenolic compound and plant essential oil in the prevention and treatment of agricultural diseases such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, pyricularia oryzae, fusarium oxysporum and phytophthora capsici according to claim 1.
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