CN114793655A - Cutting method of sildenafil holly - Google Patents
Cutting method of sildenafil holly Download PDFInfo
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- CN114793655A CN114793655A CN202210615065.1A CN202210615065A CN114793655A CN 114793655 A CN114793655 A CN 114793655A CN 202210615065 A CN202210615065 A CN 202210615065A CN 114793655 A CN114793655 A CN 114793655A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
Abstract
The invention relates to a cuttage method of sildenafil holly, belonging to the technical field of plant cultivation, (1) collecting a rejuvenation branch of the sildenafil holly for disinfection; (2) pretreating the disinfected tender branches in the step (1) to obtain cutting seedlings; (3) cutting the cutting seedling in a seedbed containing a cutting matrix formed by mixing perlite, garden soil, plant ash and cotton seed powder; (4) and (4) obtaining culture seedlings after the first stage, the second stage and the third stage after cuttage. The invention provides a cuttage method of Stephen holly, and the Stephen holly prepared by the method has high survival rate, short growth cycle and good genetic stability, and is beneficial to large-scale popularization and planting.
Description
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a cutting method of sildenafil holly.
Background
The sildenafil holly belongs to evergreen trees, is a good variety cultivated in national botanical gardens of America, is a hybrid of British holly and Chinese holly, and has a cultivation history of hundreds of years in the America. The leaves are glossy and dark green, with thorns on the front. In Nanjing area, the flowering period is from 2 late months to 3 early months, the fruit period is from 4 months to 2 months in the next year, the fruit yield is large, the fruit ripening period is late, the fruit bearing time is long, the color of the country is bright red and beautiful, birds like to eat in winter, the adult big trees are 6-8 meters high, the tree crowns are naturally conical, the crown width can reach 4 meters, and the tree is suitable for street trees, garden landscape trees, hedgerow, Christmas trees, bonsai shapes and the like, and the wood is hard and is suitable for engraving artworks.
The American Stefin holly has wide suitable growing area, is cold-resistant, drought-resistant, saline-alkali resistant and barren-resistant, is an eco-friendly tree species, and can be used as honey source and bird winter food. The growth is rapid, and the breast diameter of the five-year-old nursery stock can reach 6-8 centimeters. The pruning resistance is good, and the nursery stock is suitable for shaping; less plant diseases and insect pests, and is suitable for maintenance. The male and female plants can be self-pollinated and fructified, and the fruit has large quantity, bright color, strong dryness and no lodging. In order to enrich the forest tree species resources of China and meet the market demand, the cutting method of the sildenafil holly is provided.
Disclosure of Invention
The invention aims to solve the problems and provide a method for cutting the silvestre ilex.
The invention realizes the purpose through the following technical scheme:
the invention provides a cutting method of sildenafil hollies, which comprises the following steps:
(1) collecting the rejuvenation branch of silifen for disinfection;
(2) pretreating the disinfected tender branches in the step (1) to obtain cutting seedlings;
(3) cutting the cutting seedling in a seedbed containing a cutting matrix formed by mixing perlite, garden soil, plant ash and cottonseed powder;
(4) after cutting, obtaining culture seedlings through a first stage, a second stage and a third stage;
wherein, the first stage: keeping the temperature of the seedbed at 30-35 ℃, keeping the humidity of the seedbed at 100%, and irradiating for 14h at the illumination intensity of 2500Lux every day until the cutting seedlings take root and then entering the second stage; and a second stage: keeping the temperature of the seedbed at 25-30 ℃, reducing the humidity of the seedbed to 50% at the rate of 3%/d, enhancing the illumination intensity to 100000Lux/d at the rate of 1500Lux/d, and irradiating for 8 hours every day until a great amount of cutting seedlings sprout and enter a third stage; and a third stage: keeping the temperature of the seedbed at 20-25 ℃, keeping the humidity of the seedbed at 50-100%, irradiating for 12h at the illumination intensity of 2500-.
As a further optimization scheme of the invention, the step (1) of collecting the rejuvenation branch of Stephan, which is annual, healthy and free of any diseases and pests, with bud and is not lignified, is specifically the step of collecting the rejuvenation branch of Stephan.
As a further optimization scheme of the invention, the pretreatment in the step (2) comprises the following specific steps: the collected rejuvenation branch of holly is cut into 5-8cm length containing 1-2 nodes, and the bud and 1-2 leaves at the top are retained and soaked in growth promoting culture medium.
As a further optimization scheme of the invention, the components of the growth promoting culture medium comprise 1/2WPM + IBA0.4-0.8mg/L + NAA0.4-1.2mg/L + sugar 20g/L + agar 5 g/L.
As a further optimization of the invention, the WPM medium contains ingredients including 370mg/L MgSO 4 、950mg/L KSO 4 、170mg/L KH 2 PO 4 、400mg/L NH 3 NO 3 、556mg/L Ca(NO 3 ) 2 ·4H 2 O、72mg/L CaCl 2 、16.9mg/L MnSO 4 、8.6mg/L ZnSO 4 ·7H 2 O、6.2mg/L H 3 BO 3 、0.25mg/L Na 2 MoO 4 、0.25mg/L CuSO 4 ·5H 2 O、37.3mg/L Na 2 -EDTA、27.8mg/L FeSO 4 ·7H 2 O, 2mg/L glycine, 1.0mg/L thiamine hydrochloride, 0.5mg/L pyridoxine hydrochloride, 0.5mg/L nicotinic acid, 100mg/L inositol.
As a further optimization scheme of the invention, the cutting season of the step (3) is 7 months in summer.
As a further optimization scheme of the invention, in the step (3), the matrix is prepared from perlite, garden soil, plant ash and cottonseed meal according to a volume ratio of 1: 3: (0.5-1.5): (0.2-0.3) mixing.
In the step (4), in the third stage, the external ambient air is introduced every day, and 1500 times of diluted solution of the mixture of the dicofol and the prochloraz with equal volume is sprayed once in the morning and at night.
The invention has the beneficial effects that: the Stephen holly cuttage method provided by the invention has the advantages that the Stephen holly obtained by the method is high in survival rate, disease and insect resistant, short in growth cycle and good in growth stability, and is beneficial to large-scale popularization and planting.
Detailed Description
The present application will now be described in further detail with reference to the drawings, it should be noted that the following detailed description is given for illustrative purposes only and is not to be construed as limiting the scope of the present application, as those skilled in the art will be able to make numerous insubstantial modifications and adaptations to the present application based on the above disclosure.
Example 1
The embodiment provides a method for cutting silvestre ilex, which comprises the following steps:
(1) collecting the silifen holly rejuvenation branch, which is a silifen holly rejuvenation branch which is grown in the same year, healthy, free of any diseases and pests, provided with buds and not lignified, and placing the silifen holly rejuvenation branch in a carbendazim solution diluted by 550 times with 450-;
(2) cutting the disinfected tender branches in the step (1) into 5-8cm long branches with 1-2 nodes, reserving 1-2 leaves on the upper buds and the top ends, and soaking the tender branches in a growth promoting culture medium for 10min, wherein the growth promoting culture medium comprises 1/2WPM, IBA0.8mg/L, NAA0.8mg/L, white sugar 20g/L and agar 5g/L to obtain cutting seedlings; wherein the WPM medium contains 370mg/LMgSO 4 、950mg/LKSO 4 、170mg/LKH 2 PO 4 、400mg/LNH 3 NO 3 、556mg/LCa(NO 3 ) 2. 4H 2 O、72mg/LCaCl 2 、16.9mg/LMnSO 4 、8.6mg/LZnSO 4 .7H 2 O、6.2mg/LH 3 BO 3 、0.25mg/LNa 2 MoO 4 、0.25mg/LCuSO 4 .5H 2 O、37.3mg/LNa 2 -EDTA、27.8mg/LFeSO 4 .7H 2 O, 2mg/L glycine, 1.0mg/L thiamine hydrochloride, 0.5mg/L pyridoxine hydrochloride, 0.5mg/L nicotinic acid, 100mg/L inositol.
(3) Preferably, in 7-month middle ten days in summer, cutting the cutting seedlings in a manner that the volume ratio of perlite, garden soil, plant ash and cotton seed powder is 1: 3: 0.8: 0.5 in the seedbed of the mixed cuttage matrix;
(4) after cutting, obtaining culture seedlings through a first stage, a second stage and a third stage;
wherein, the first stage: keeping the temperature of the seedbed at 30-35 ℃, keeping the humidity of the seedbed at 100%, irradiating for 14h at the illumination intensity of 2500Lux every day until the cuttage seedlings take root for 20-45 d, and entering the second stage; and a second stage: maintaining the temperature of the seedbed at 25-30 ℃, reducing the humidity of the seedbed to 50% at the rate of 3%/d, enhancing the illumination intensity to 100000Lux/d at the rate of 1500Lux/d, and irradiating for 8 hours every day until a large number of cutting seedlings grow new buds at 60-90 d and then entering a third stage; and a third stage: keeping the temperature of the seedbed at 20-25 ℃, keeping the humidity of the seedbed at 50-100%, irradiating for 12h at the illumination intensity of 2500-.
The conditions of the third stage daily seedbed are shown in the table:
example 2
The components of the growth promoting culture medium except the components of the growth promoting culture medium in the step (2) comprise 1/2WPM, 0.6mg/L IBA, 0.8mg/L NAA, 20g/L white sugar and 5g/L agar;
(4) after cutting, obtaining culture seedlings through a first stage, a second stage and a third stage;
wherein, the first stage: keeping the temperature of the seedbed at 30-35 ℃, keeping the humidity of the seedbed at 100%, irradiating for 14h at the illumination intensity of 2500Lux every day until the cuttage seedlings take root for 30-60 d, and entering the second stage; and a second stage: keeping the temperature of the seedbed at 25-30 ℃, reducing the humidity of the seedbed to 50% at the rate of 3%/d, enhancing the illumination intensity to 100000Lux/d at the rate of 1500Lux/d, and irradiating for 8 hours every day until a large number of cutting seedlings grow new buds at 90-120 d and then entering the third stage; and a third stage: keeping the temperature of the seedbed at 20-25 ℃, keeping the humidity of the seedbed at 50-100%, irradiating for 12h at the illumination intensity of 2500-;
the rest of the procedure was the same as in example 1.
Example 3
The components of the growth promoting culture medium except the components of the growth promoting culture medium in the step (2) comprise 1/2WPM, 0.4mg/L IBA, 1.2mg/L NAA, 20g/L white sugar and 5g/L agar;
wherein, the first stage: keeping the temperature of the seedbed at 30-35 ℃, keeping the humidity of the seedbed at 100%, irradiating for 14h at the illumination intensity of 2500Lux every day until the cuttage seedlings take root for 15-25 d, and entering the second stage; and a second stage: keeping the temperature of the seedbed at 25-30 ℃, reducing the humidity of the seedbed to 50% at the rate of 3%/d, enhancing the illumination intensity to 100000Lux/d at the rate of 1500Lux/d, and irradiating for 8 hours every day until a large number of cutting seedlings grow new buds at 45-80 d and then entering a third stage; and a third stage: keeping the temperature of the seedbed at 20-25 ℃, keeping the humidity of the seedbed at 50-100%, irradiating for 12h at the illumination intensity of 2500-;
the rest of the procedure was the same as in example 1.
Example 4
In the third stage except the step (4), the third stage: keeping the temperature of the seedbed at 20-25 ℃, keeping the humidity of the seedbed at 50-100%, irradiating for 12h at the illumination intensity of 2500-. The rest of the procedure was the same as in example 3.
Example 5
Removing step (3): preferably, in 7-month middle ten days in summer, cutting the cutting seedlings in a manner that the volume ratio of perlite, garden soil, plant ash and cotton seed powder is 1: 3: 0.8: 0.2 in the seedbed of the mixed cuttage matrix; the rest of the procedure was the same as in example 3.
Comparative example 1
Except the components of the growth promoting culture medium in the step (2), including WPM, IBA0.4mg/L, NAA0.4mg/L, white sugar 20g/L and agar 5 g/L;
(4) after cutting, obtaining culture seedlings through a first stage, a second stage and a third stage;
wherein, the first stage: keeping the temperature of the seedbed at 30-35 ℃, keeping the humidity of the seedbed at 100%, irradiating for 14h at the illumination intensity of 2500Lux every day until the cuttage seedlings take root for 30-45 d, and entering the second stage; and a second stage: keeping the temperature of the seedbed at 25-30 ℃, reducing the humidity of the seedbed to 50% at the rate of 3%/d, enhancing the illumination intensity to 100000Lux/d at the rate of 1500Lux/d, and irradiating for 8 hours every day until a large number of cutting seedlings of 105-135 d emit new buds and then entering the third stage; and a third stage: keeping the temperature of the seedbed at 20-25 ℃, keeping the humidity of the seedbed at 50-100%, irradiating for 12h at the illumination intensity of 2500-;
the rest of the procedure was the same as in example 3.
Comparative example 2
Removing step (3): preferably, in 7-month middle ten days in summer, cutting the cutting seedlings in a mode that the volume ratio of perlite to garden soil is 1: 3 in the seedling bed of the mixed cuttage substrate; the rest of the procedure was the same as in example 3.
Comparative example 3
Removing step (4): after cuttage, keeping the temperature of the seedbed at 30-35 ℃, keeping the humidity of the seedbed at 100%, and irradiating for 14h at the illumination intensity of 5000Lux every day for 6-8 months to obtain cultured seedlings;
the rest of the procedure was the same as in example 3.
The cutting seedlings cultivated in examples 1 to 5 and comparative examples 1 to 3 were transplanted to the natural environment.
And (4) survival rate testing: observing the survival condition of 50 cutting seedlings cultured in examples 1-5 and comparative examples 1-3 in 3 months after being transplanted into a natural environment, and recording the survival rate;
as can be seen from examples 1-3 and comparative example 1 in the above table, the survival rate of the ilex purpurea is the highest when the growth promoting medium components comprise 1/2WPM + IBA0.4mg/L + NAA1.2mg/L + white sugar 20g/L + agar 5g/L, and example 3, the content change of specific components in the growth promoting medium components 1/2WPM + IBA0.4-0.8mg/L + NAA0.4-1.2mg/L + sugar 20g/L + agar 5g/L affects the survival rate of the ilex purpurea cuttage; by comparing example 3 with example 4, the survival rate of example 4 is higher than that of example 3, and it is presumed that in example 4, insect prevention measures may be taken during the cutting process, so that the survival rate is increased; comparing comparative example 2 with example 3, the survival rate of comparative example 2 is low, and presumably is increased due to the difference of culture mediums; compared with the comparative example 3 and the example 3, the same illumination, humidity and temperature are adopted throughout the comparative example 3, so that the winter green seedlings are not easy to survive when being transplanted to the external environment.
And (3) testing disease and pest resistance, selecting the ilex chinensis seedlings which survive after being transplanted into the natural environment for two months, respectively selecting 50 cutting seedlings cultured in examples 1-5 and comparative examples 1-3, contacting red spiders, aphids and ilex purpurea leaves suffering from brown leaf disease, and recording the survival condition of the ilex purpurea seedlings after 15 days.
| Item | Non-dyed/% | Brown leaf disease only/%) | Infection with insect pests/%) | Both contain/%) |
| Example 1 | 88 | 6 | 4 | 4 |
| Example 2 | 88 | 4 | 4 | 6 |
| Example 3 | 90 | 4 | 4 | 2 |
| Example 4 | 98 | 2 | 0 | 0 |
| Example 5 | 84 | 4 | 4 | 8 |
| Comparative example 1 | 82 | 6 | 4 | 8 |
| Comparative example 2 | 64 | 10 | 4 | 20 |
| Comparative example 3 | 80 | 12 | 2 | 6 |
As can be seen from the above table, example 4 is the most preferred example against disease and pest, and the test groups of examples 1-3 were similarly affected by disease and pest, but there were some fluctuations in example 2 presumably due to the effect of IBA in the medium, and it was presumed that the effects of the matrix soil were likely to be observed by comparing comparative example 2 with example 3 and example 5.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (8)
1. A cutting method of sildenafil hollies is characterized by comprising the following steps:
(1) collecting and sterilizing the rejuvenation branch of silifenum rubrum;
(2) pretreating the disinfected tender branches in the step (1) to obtain cutting seedlings;
(3) cutting the cutting seedling in a seedbed containing a cutting matrix formed by mixing perlite, garden soil, plant ash and cotton seed powder;
(4) after cuttage, culturing in a first stage, a second stage and a third stage to obtain culture seedlings;
wherein, the first stage: keeping the temperature of the seedbed at 30-35 ℃, keeping the humidity of the seedbed at 100%, and irradiating for 14h at the illumination intensity of 2500Lux every day until the cutting seedlings take root and then entering the second stage; and a second stage: keeping the temperature of the seedbed at 25-30 ℃, reducing the humidity of the seedbed to 50% at the rate of 3%/d, enhancing the illumination intensity to 100000Lux/d at the rate of 1500Lux/d, and irradiating for 8 hours every day until a great amount of cutting seedlings sprout and enter a third stage; and a third stage: keeping the temperature of the seedbed at 20-25 ℃, keeping the humidity of the seedbed at 50-100%, irradiating for 12h at the illumination intensity of 2500-.
2. The method of claim 1, wherein the collection of the rejuvenation shoot of Stefin ilex in step (1) is a collection of a current-year, healthy shoot free of any disease and insect pests, bud-bearing and not yet lignified Stefin ilex.
3. The method for cutting sildenafil hollies according to claim 1, wherein the pretreatment in the step (2) comprises the following specific steps: the collected rejuvenation branches of the holly are cut into 5-8cm long with 1-2 nodes, and the upper buds and 1-2 leaves at the top are reserved and soaked in the growth promoting medium.
4. The method for cutting sildenafil holly root according to claim 3, wherein the growth promoting medium comprises 1/2WPM + IBA0.4-0.8mg/L + NAA0.4-1.2mg/L + sugar 20g/L + agar 5 g/L.
5. The method of claim 4, wherein the WPM medium contains ingredients including 370mg/L MgSO 4 、950mg/L KSO 4 、170mg/L KH 2 PO 4 、400mg/L NH 3 NO 3 、556mg/L Ca(NO 3 ) 2. 4H 2 O、72mg/L CaCl 2 、16.9mg/L MnSO 4 、8.6mg/L ZnSO 4 ·7H 2 O、6.2mg/L H 3 BO 3 、0.25mg/L Na 2 MoO 4 、0.25mg/L CuSO 4 ·5H 2 O、37.3mg/L Na 2 -EDTA、27.8mg/L FeSO 4 ·7H 2 O, 2mg/L glycine, 1.0mg/L thiamine hydrochloride, 0.5mg/L pyridoxine hydrochloride, 0.5mg/L nicotinic acid, 100mg/L inositol.
6. The method for cutting sildenafil hollies according to claim 1, wherein the cutting season of step (3) is 7 months in summer.
7. The method for cutting sildenafil hollies according to claim 1, wherein in the step (3), the substrate is prepared from perlite, garden soil, plant ash and cotton seed powder according to a volume ratio of 1: 3: (0.5-1.5): (0.2-0.3) mixing.
8. The method for cutting sildenafil ilex according to claim 1, wherein in the third stage of step (4), ambient air is introduced daily, and equal volumes of 1500 times diluted solution of the mixture of dicofol and prochloraz are sprayed in the morning and evening.
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| CN202210615065.1A CN114793655A (en) | 2022-06-01 | 2022-06-01 | Cutting method of sildenafil holly |
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| CN202210615065.1A CN114793655A (en) | 2022-06-01 | 2022-06-01 | Cutting method of sildenafil holly |
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