CN114791505A

CN114791505A - Full-automatic detection platform and detection method

Info

Publication number: CN114791505A
Application number: CN202110106471.0A
Authority: CN
Inventors: 严义勇; 蔡振庆; 肖雄文; 刘运旺; 朱海
Original assignee: SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
Current assignee: SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
Priority date: 2021-01-26
Filing date: 2021-01-26
Publication date: 2022-07-26

Abstract

The application provides a full-automatic detection platform and a detection method. The application provides a testing platform includes: the detection platform is provided with a first storage area and a second storage area, the first storage area is used for storing the sample storage piece, and the second storage area is used for storing the detection material consumption piece; the sample storage piece is provided with a storage space and an access communicated with the storage space, and the storage space is used for storing a sample to be detected; the detection consumable part is used for receiving a sample to be detected and detecting the sample to be detected; the sample suction and blowing mechanism is used for sucking a sample to be detected from the inlet and the outlet and blowing the sample to be detected to the detection consumable part; a manipulation mechanism for operating on a horizontal plane and a vertical plane to manipulate the test consumable and the sample suction and blowing mechanism; a control system having a sample detection program for controlling the manipulation mechanism to execute an execution operation corresponding to the sample detection program according to the sample detection program; and the reading analyzer is used for reading, analyzing and outputting the detection result of the detection consumable part.

Description

Full-automatic detection platform and detection method

Technical Field

The application belongs to the technical field of biological sample detection, and particularly relates to a full-automatic detection platform and a detection method.

Background

As is well known, food safety is gradually becoming one of important topics concerned by people, biological sample detection of food is taken as an important means for judging food safety, and a corresponding detection method and platform thereof are applied to key links of food safety supervision.

In the prior art, corresponding standardized operation requirements are formulated respectively for the detection processes of different biological samples, so that the detection error is reduced by the standardized operation, and the aim of improving the accuracy of the detection result is fulfilled. However, because of the manual participation in the detection process partially or completely, and the real-time requirement of some types of detection, and the comprehensive influence of the factors such as the working environment, the state, the detection amount, the detection frequency and the like, in the sample detection process, the standard detection cannot be achieved inevitably due to the incorporation of the artificial factors, and the error of the detection result changes with the difference of the incorporation factors, thereby further causing the difficulty in counting and analyzing the detection result. Moreover, the detection requirements and the operation standards of different biological samples are different, the same operation standard is difficult to meet the detection of various biological samples, the generalization degree of the detection standard is low, and the manual detection cost is high.

Disclosure of Invention

An object of the embodiment of the application is to provide a full-automatic detection platform, which has the advantages of high-precision detection, various detection and low detection cost.

In order to achieve the purpose, the technical scheme adopted by the application is as follows: providing a fully automated testing platform comprising:

the detection platform is at least provided with a first storage area and a second storage area, the first storage area is used for storing sample storage pieces, and the second storage area is used for storing detection consumable pieces;

the sample storage piece is provided with a storage space and an inlet and an outlet communicated with the storage space, and the storage space is used for storing a sample to be detected;

the detection consumable part is used for receiving a sample to be detected and detecting the sample to be detected;

the sample suction and blowing mechanism is used for sucking a sample to be detected from the inlet and the outlet and blowing the sample to be detected to the detection material consumption piece;

a handling mechanism for handling the detection consumable part and the sample sucking and blowing mechanism;

a control system having a sample detection program for controlling the manipulation mechanism to execute an execution operation corresponding to the sample detection program according to the sample detection program;

and the reading analyzer is used for reading, analyzing and outputting the detection result of the detection consumable part.

In one embodiment, the sample sucking and blowing mechanism comprises a sucking and blowing driver and a sucking and blowing head which is adapted to the sucking and blowing driver;

the detection platform is provided with a third storage area, the third storage area is used for storing the suction and blowing head, and the control mechanism is also used for controlling the suction and blowing driver to be connected with the suction and blowing head so as to drive the suction and blowing head to suck and blow a sample to be detected.

In one embodiment, the suction-blowing driver is internally provided with a control circuit, the control circuit is electrically connected to the control system, and the control system is further configured to adjust the control circuit according to the sample detection program to control the output power of the suction-blowing controller.

In one embodiment, the testing platform further comprises a testing seat, the testing seat comprising:

the upper surface of the clamping seat is provided with a sunken placing area, and the placing area is used for placing detection consumable parts;

a position sensor for detecting in real time whether the detection consumable part is placed in the placement area;

an auxiliary positioning element comprising a fixed part and an abutment part coupled to the fixed part and movable with respect to the fixed part, the abutment part being intended to limit the position of the detection consumable in the placement area.

In one embodiment, the operating mechanism comprises a mechanical arm arranged on the detection table and a clamping jaw coupled to the tail end of the mechanical arm, wherein the clamping jaw is provided with a first clamping end and a second clamping end which are oppositely arranged;

the mechanical arm is used for operating on a horizontal plane and a vertical plane to operate the clamping jaw, the first clamping end is adapted to the detection material consumption piece, and the second clamping end is adapted to the sample suction and blowing mechanism.

In one embodiment, the third storage area is provided with a support assembly;

the supporting component comprises a supporting seat and a storage seat which is elastically supported on the supporting seat, a storage groove is formed in the storage seat, and the storage groove is used for storing the suction and blowing head.

In one embodiment, the supporting assembly further comprises a supporting plate, and the supporting plate is elastically supported on the supporting seat;

the adjustable storage device is characterized in that an adjusting stand column is arranged on the supporting plate, an adjusting through hole is formed in the storage seat, the radial size of the adjusting through hole is larger than that of the adjusting stand column, and the storage seat is supported on the supporting plate and sleeved with the adjusting through hole.

In one embodiment, the first storage area is provided with at least one storage box, the storage box is provided with a plurality of storage rooms which are arranged according to a regular array and used for storing the sample storage pieces;

the second storage area is provided with at least one storage bin, the storage bin is provided with a plurality of storage ports which are arranged according to a regular array, and the storage ports are used for storing the detection consumable parts.

In one embodiment, the detection platform further comprises a suction and blowing head detaching piece;

the suction blowing head detaching piece at least comprises a first piece portion and a second piece portion which are spaced relatively, the distance between the first piece portion and the second piece portion is smaller than the radial size of the suction blowing head, the distance between the first piece portion and the second piece portion is larger than the radial size of the suction blowing driver, and the first piece portion and the second piece portion are used for applying force to the suction blowing head from the end face of the suction blowing head so as to enable the suction blowing head to be separated from the suction blowing driver.

In one embodiment, the detection consumable is any one of:

-a test card;

-a cuvette;

-a centrifuge tube;

-cell culture plates.

Compared with the prior art, the beneficial effects of the full automation testing platform that this application provided lie in:

firstly, the detection platform provided by the application realizes full-automatic standardized operation, avoids manual operation from being mixed into the detection process, and can realize high-precision detection and high-efficiency detection;

secondly, the waiting period of the test is effectively shortened, the detection work can be carried out all weather, the production period is shortened, and the material circulation efficiency is improved;

thirdly, when the sample to be detected is a test sample containing pathogenic bacteria, the automatic test process can prevent the pollution to the labor personnel and improve the detection safety;

fourthly, in the operation process of some samples, particularly when testing the microbiological correlation items, the phenomenon that the samples and the tests are interfered to obtain wrong results due to pollution sources of the samples or clothes caused by the fact that personnel stay in different environments occasionally occurs, and the automatic operation process can effectively reduce and eliminate the pollution to the samples;

fifthly, the detection platform can be configured with various sensors and camera equipment as required, and based on the visual feedback configuration platform, the detection process of the visual feedback auxiliary platform is used for preventing the process misoperation and reviewing, analyzing and counting the process;

according to the detection platform provided by the application, the preorder station is effectively linked with the subsequent station, so that the linking cost of the process is reduced on the whole, the continuity and traceability of data are realized, the artificial data loss and the statistical cost of the linking process are reduced, and the data transmission time is saved no matter whether the operation or the data are operated;

seventhly, the testing platform that this application provided, accessible increase peripheral part such as dustcoat, in the narrow range and low-cost realization environmental control, like cleanliness factor, temperature humidity, damage nature physical factor (optics, magnetism, radiation etc.) etc. when realizing more accurate test procedure, can develop the human intolerable destructive experiment of special requirements, obtain more complete scientific data.

It is another object of the present application to provide an assay method corresponding to the fully automated assay platform as described above, which comprises:

adding a sample to be detected into a sample storage part;

storing the sample storage member in a first storage area of a test station;

storing the detection consumable part corresponding to the sample to be detected in a second storage area of the detection platform;

starting a sample detection program corresponding to the sample to be detected;

starting a reading analyzer;

wherein the sample detection procedure comprises:

controlling a handling mechanism to take the detection consumable part from the second storage area and position the detection consumable part at a certain spatial position;

controlling the operating mechanism to operate the sample suction and blowing mechanism to suck the sample to be detected from the sample storage part in the first storage area;

controlling the operating mechanism to operate the sample sucking and blowing mechanism to blow and cover the sucked sample to be detected on the detection material consumption piece;

and controlling the operating mechanism to take the detection consumable parts covered with the samples to be detected to the reading analyzer.

In one embodiment, the sample sucking and blowing mechanism comprises a sucking and blowing driver and a sucking and blowing head adapted to the sucking and blowing driver;

the detection method further comprises the following steps:

storing the suction-blowing head in a third storage area of the detection table;

control operating device takes sample suction-blowing mechanism and operates sample suction-blowing mechanism and absorb in the sample storage piece of first memory area the sample that awaits measuring includes:

controlling the operating mechanism to take the suction-blowing driver from the placing station and operating the suction-blowing driver to be connected with the suction-blowing head;

and controlling the operating mechanism to operate the suction-blowing driver connected with the suction-blowing head to suck the sample to be tested from the sample storage part of the first storage area.

In one embodiment, the controlling the operating mechanism to take the suction-blowing driver from the placing station and operate the suction-blowing driver to be coupled to the suction-blowing head includes:

and controlling the control mechanism to control the suction-blowing driver from top to bottom and press the suction-blowing driver to be sleeved on the suction-blowing head.

In one embodiment, the detection platform further comprises a suction and blowing head detaching piece; the suction blowing head detaching piece at least comprises a first piece part and a second piece part which are oppositely spaced, the distance between the first piece part and the second piece part is smaller than the radial dimension of the suction blowing head, and the distance between the first piece part and the second piece part is larger than the radial dimension of the suction blowing driver;

before the control mechanism is used for controlling the detection consumable parts covered with the samples to be detected to take to the reading analyzer, the method also comprises the following steps;

and controlling a manipulation mechanism to manipulate the suction-blowing driver connected with the suction-blowing head to enter between the first sheet part and the second sheet part, and manipulating the suction-blowing driver from bottom to top so that the first sheet part and the second sheet part apply force to the suction-blowing head from the end face of the suction-blowing head to separate from the suction-blowing driver.

In one embodiment, after the controlling the handling mechanism to take the consumable part from the second storage area and position the consumable part in a certain spatial position, and before the controlling the handling mechanism to take the sample sucking and blowing mechanism and operate the sample sucking and blowing mechanism to suck the sample to be tested from the sample storage part in the first storage area, the method further comprises:

detecting, by a position sensor, current position information of the detected consumable part and sending the position information to the control system;

the control system receives the position information and controls the movement of the abutment of the auxiliary positioning element in dependence on the position information to define the current position of the detecting consumable part.

In one embodiment, the detection method further comprises:

controlling a handling mechanism to take the detection consumable part from the second storage area and place the detection consumable part in a specified placement area;

and controlling the operating mechanism to place the sample sucking and blowing mechanism back to the placing station of the sample sucking and blowing mechanism.

Compared with the beneficial effects of the prior art, the full-automatic detection method provided by the application has the beneficial effects that compared with the full-automatic detection platform provided by the application, the full-automatic detection platform provided by the application has the beneficial effects that the full-automatic detection platform is not repeated.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.

FIG. 1 is a schematic diagram of a fully automated testing platform provided in an embodiment of the present application;

FIG. 2 is a coaxial side view of FIG. 1;

FIG. 3 is a first schematic view of a support assembly provided in accordance with an embodiment of the present application;

FIG. 4 is a second schematic view of a support assembly provided in accordance with an embodiment of the present application, wherein the storage seat is omitted;

fig. 5 is a schematic view of a detection seat according to an embodiment of the present application.

Wherein, in the figures, the respective reference numerals:

10-a detection table; 20-a mechanical arm; 30-a clamping jaw; 40-a control system; 50-reading analyzer; 60-a bottom plate;

11-a storage box; 12-a sample storage member;

21-a storage bin; 22-detecting a consumable part;

31-a storage seat; 32-adjusting through holes; 33-a storage tank; 34-a suction and blowing head; 35-a support seat; 36-a support plate; 37-adjusting the upright post; 38-a spring;

41-card seat; 42-a position sensor; 43-an auxiliary positioning member;

51-a first sheet portion; 52-second sheet portion.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects to be solved by the present application clearer, the present application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of and not restrictive on the broad application.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.

It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings that is solely for the purpose of facilitating the description and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the present application.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

The full-automatic detection platform and the detection method provided by the embodiment of the application are now described.

Referring to fig. 1 and fig. 2, the fully automated testing platform provided in the present application includes:

the inspection table 10 is provided with a first storage area, a second storage area, a third storage area and a placement station; wherein the first storage area is used for storing the sample storage member 12, the second storage area is used for storing the detection material consumption member 22, the third storage area is used for storing the suction and blowing head 34, and the placing station is used for placing the suction and blowing driver;

the sample storage part 12 is provided with a storage space and an access communicated with the storage space, and the storage space is used for storing a sample to be detected;

a test consumable 22 for receiving and testing a sample to be tested;

a sample suction and blowing mechanism for sucking a sample to be tested from the entrance and the exit and blowing the sample to be tested to the test consumable part 22; wherein the sample sucking and blowing mechanism comprises a sucking and blowing driver and a sucking and blowing head 34 adapted to the sucking and blowing driver;

a manipulation mechanism for operating on a horizontal plane and a vertical plane to manipulate the detecting consumable part 22 and the sample sucking and blowing mechanism horizontally and vertically. When the sample suction and blowing mechanism comprises a suction and blowing driver and a suction and blowing head 34 adapted to the suction and blowing driver, the manipulating mechanism is further used for manipulating the suction and blowing driver to be coupled to the suction and blowing head 34 to drive the suction and blowing head 34 to suck and blow the sample to be tested;

a control system 40 having a sample detection program for controlling the manipulation mechanism to perform an execution operation corresponding to the sample detection program according to the sample detection program;

a reading analyzer 50 for reading, analyzing and outputting the detection result of the detection consumable part 22.

When the sample storage device is used specifically, a sample to be detected is added into the sample storage piece, the sample storage piece is stored in a first storage area of a detection table, a detection material consumption piece corresponding to the sample to be detected is stored in a second storage area of the detection table, a suction-blowing head is stored in a third storage area of the detection table, a suction-blowing driver is placed at a placing station of the detection table, a sample detection program corresponding to the sample to be detected is started, and a reading analyzer is started;

wherein the sample detection procedure comprises:

controlling the operating mechanism to take the detection consumable parts from the second storage area and place the detection consumable parts in the designated placement area; controlling the operating mechanism to take the sample suction-blowing mechanism from the placing station and operating the sample suction-blowing mechanism to suck the sample to be detected from the sample storage part in the first storage area; controlling the operating mechanism to take the suction-blowing driver from the placing station and operating the suction-blowing driver to be connected with the suction-blowing head; controlling an operating mechanism to operate a suction-blowing driver connected with a suction-blowing head to suck a sample to be detected from a sample storage part of the first storage area; controlling the control mechanism to control the suction-blowing driver to blow and cover the sucked sample to be detected on the detection material consumption piece; controlling the operating mechanism to abandon the suction-blowing head and placing the suction-blowing driver back to the placing station; and controlling the operating mechanism to take the detection consumable parts coated with the samples to be detected from the designated placing area to the reading analyzer.

It should be understood that in the embodiment of the present application, the operating mechanism is used for operating on a horizontal plane and a vertical plane, wherein the vertical plane includes the vertical plane and an inclined vertical plane, that is, the inclined vertical plane is: the force of the horizontal plane and the force of the vertical plane are combined to generate a plane which forms an included angle with the horizontal plane and the vertical plane. Meanwhile, the operating mechanism provided by the embodiment of the present application is used for operating on a horizontal plane and a vertical plane, and only refers to a spatial plane where a certain time point is located, and actually, the motion track of the operating mechanism may be a curve or a curved surface, which should fall within the scope of the present application for operating on the horizontal plane and the vertical plane.

Wherein, in this application embodiment, preferred sample suction-blowing mechanism includes suction-blowing driver and the suction-blowing head 34 who adapts to suction-blowing driver, and both place independently, so, suction-blowing head can adapt to the different sample that awaits measuring, and to same kind of sample that awaits measuring and same kind suction-blowing head, can abandon the suction-blowing head that uses, and suction-blowing driver remains to this reduce cost.

Of course, in other embodiments, the sample sucking and blowing mechanism may be of an integral structure and may be integrally disposed, and the manipulating mechanism may directly manipulate the whole sample sucking and blowing mechanism, and may be reusable or disposable.

In the embodiment of the present application, the suction-blowing driver is preferably a pipette, and the suction-blowing head is preferably a gun head.

In other embodiments, the liquid transfer device may be replaced by a liquid pump, and the common feature of both of them is that they can be used for sucking and blowing liquid. In one embodiment, the preferred mode is replaced by a mode in which the pump and the lance tip are butted, and then the tail end of the pump needs to be designed into a mode matched with the inner wall of the lance tip so as to facilitate the butting of the pump and the lance tip. In one embodiment, replacing the preferred embodiment with a pump and syringe combination requires the addition of a cleaning station to clean the ends of the pump and syringe combination to prevent cross-contamination of the test samples.

In the first embodiment, when the preferred embodiment is a liquid pump, the pump and the liquid suction part (the part combined with the gun head or the needle tube) can be designed as a whole, and at this time, the clamping jaws of the operating mechanism clamp the liquid pump; in one embodiment, when the liquid pump is provided as a preferred embodiment, the pump and the liquid suction portion (a portion combined with the tip or the needle tube) may be designed separately, and at this time, the holding jaw of the operating mechanism only holds the liquid suction portion. The integral design has the advantages of high precision and heavy weight; the split design has the advantages of light weight and low precision.

In the embodiment of the application, the manipulation mechanism can move the pipettor from one spatial position to another spatial position, and the pipettor can be placed at a certain position of the detection table, or the pipettor can be directly arranged on the manipulation mechanism.

In one embodiment, a control circuit is arranged in the suction-blowing driver, the control circuit is electrically connected to the control system, and the control system is further configured to adjust the control circuit according to the sample detection program to control the output power of the suction-blowing controller. In the preferred scheme, control the pipettor through control circuit, can absorb and shift the liquid of test sample by ration, can effectively avoid manual operation pipettor operation in, the excessive imbibition that often appears, the hydrojet is not enough, fall the imbibition scheduling problem behind the hydrojet, improve the imbibition precision by a wide margin, promote and detect the precision.

The detection platform is provided with a platform surface, and the first storage area, the second storage area, the third storage area and the placement station are designed by taking the platform surface as a reference.

Wherein the first storage area is provided with at least one storage box 11, the storage box 11 is provided with a plurality of storage compartments arranged in a regular array for storing the sample storage members 12. The first storage area may be provided with a plurality of storage boxes 11, the plurality of storage boxes 11 are provided with a plurality of storage compartments arranged in a regular array, the structures or shapes of the storage compartments on different storage boxes are different for storing sample storage members 12 of different shapes and structures, and the sample storage members of different shapes and structures may be used for storing different kinds of samples to be tested.

Specifically, in the embodiment of the present application, a sunken space is provided on the detection table 10, the plurality of storage boxes 11 are disposed in the sunken space, and the plurality of storage boxes 11 are sequentially disposed along a length direction of the sunken space and close to each other.

Storage box 11 has between the storage of regular spread, can carry out the arrangement of regularization to the test sample and place, and the clamp of the operating mechanism of being convenient for gets and pipettor gets liquid, and pier, camera can also be settled to sweep above it, take notes the discrepancy of sample, perhaps discerns the sign above the sample, judges the position, the type of sample according to this, realizes and plans the matching of test queue, the result is traced to the source, process tracking and information input. Meanwhile, the liquid taking process of the liquid shifter can be tracked.

And, storage box 11 still can be equipped with and incubate and vibrate the part, and the sample is carried out incubation, the oscillation of setting for match test process better. Meanwhile, some samples can be prevented from coagulation, sampling accuracy is reduced, and representativeness is not achieved.

The second storage area is provided with at least one storage bin 21, and the storage bin 21 is provided with a plurality of storage ports arranged in a regular array, and the storage ports are used for storing the detection consumable parts 22. The second storage area can set up a plurality of storage storehouses 21, and a plurality of storage storehouses all are equipped with a plurality of storage mouths of arranging according to regular array, and the storage mouth of different storage storehouses can be used to the detection consumptive material piece 22 of different types of storage.

Specifically, this application embodiment is provided with a vertical type storage storehouse 21 on detecting the platform, and storage storehouse 21 is provided with a plurality of storage mouths that arrange in proper order along the horizontal direction and a plurality of storage mouths that arrange in proper order along vertical direction.

The magazine 21 has regularly arranged storage ports to facilitate placement of the consumable parts 22, for example, test cards may be placed in a regular array to facilitate gripping by a manipulator. A scanning head, a camera and a camera can be arranged above the test card, the entering and exiting of the test card are recorded, or the identification above the test card is identified, the type of the card is sequentially judged, and the card is matched with a planned test queue and the result is traced.

Referring to fig. 3 and 4, in one embodiment, the third storage area is provided with a support assembly; the supporting component comprises a supporting seat 35 and a storage seat 31 which is elastically supported on the supporting seat 35, a storage groove 33 is formed in the storage seat 31, and the storage groove 33 is used for storing the suction head 34.

The supporting component further comprises a supporting plate 36, and the supporting plate 36 is elastically supported on the supporting seat 35; the supporting plate 36 is provided with an adjusting upright column 37, the storage seat 31 is provided with an adjusting through hole 32, the radial size of the adjusting through hole 32 is larger than that of the adjusting upright column 37, and the storage seat 31 is supported on the supporting plate 36 and the adjusting through hole 32 is sleeved on the adjusting upright column 37.

The quantity of adjusting stand 37 can be a plurality of, its top shape has certain chamfer, in order to make things convenient for the regulation through-hole 38 of storage seat 31 to get into, can in close contact with after targetting in place simultaneously, adjust stand 37 still can blow the head 34 and inhale the matching process of blowing the driver to inhaling in the storage seat 31 and lead, prevent to inhale and blow head 34 and empty, shift, effectively improve the accurate matching of inhaling and blowing the driver and inhaling the head, reduce because of the matching problem to inhaling and blowing the driver, operating mechanism's damage and process failure probability.

And, adopt spring 38 to support between storage seat 31 and the supporting seat 35, realize the buffering when suction-blow driver and suction-blow head contact, avoid hard contact to the damage of suction-blow driver and operating mechanism. Wherein the spring may be in a one-point or multi-point arrangement.

Referring to fig. 5, in an embodiment, the detection platform further includes a detection seat, and the detection seat includes:

a cassette 41, the upper surface of which is provided with a sunken placing area for placing the detection consumable part 22;

a position sensor 42 for detecting in real time whether the detection consumable piece 22 is placed in the placement area;

an auxiliary positioning element 43 comprising a fixed part and an abutment part coupled to said fixed part and movable with respect to said fixed part, said abutment part being intended to limit the position of said detection consumable 22 in said placement area.

When the system is used specifically, the current position information of the detection consumable part is detected through a position sensor and is sent to the control system; the control system receives the position information and controls the extension of the telescopic part of the auxiliary positioning element according to the position information to limit the current position of the detected consumable part.

In this embodiment, the clamping seat 41 is disposed on the inspection table, and an inclined chamfer is disposed at one end of the placement area to avoid the space when the consumable part 22 is detected to enter or exit the placement area.

The fixing part of the auxiliary positioning part 43 is disposed on one side of the card seat 41, the abutting part is designed in a telescopic manner, and can be a push-pull motor or an air cylinder, and the auxiliary positioning part contracts when the detection consumable part is placed in, extends out to abut against/press the consumable after the detection consumable part 22 is placed in, or blocks the moving track of the consumable in a short distance, so that the auxiliary positioning part is prevented from shifting in the operation process, the operation stability is improved, and the leakage is prevented.

In another embodiment, the fixing portion of the auxiliary positioning element 43 is disposed on one side of the clamping seat 41, and its abutting portion is designed to be clamped and fixed, for example, two clamping portions hinged to the fixing portion are designed, and the current position of the detecting consumable part is determined by means of the elastic element and the two clamping portions. Wherein the manner is known in the art and will not be described in detail in this application.

The position sensor 42 can determine and detect the position of the consumable part by ultrasonic, magnetic, photoelectric, pressure, etc., and determine whether the consumable part is in place or displaced, thereby monitoring the testing process.

In other embodiments, the position of the consumable part can be detected by a camera or a video camera.

In other embodiments, the test seat can be eliminated, and the consumable part can be positioned at a certain position by the operating mechanism itself, for example, the consumable part can be suspended and positioned above the test platform, or can be placed at a certain position on the table top of the test platform.

In one embodiment, the handling mechanism comprises a robot arm 20 disposed at the inspection station and a clamping jaw 30 coupled to a distal end of the robot arm 20, the clamping jaw 30 having a first clamping end and a second clamping end disposed opposite to each other;

the robot arm 20 is configured to operate in a horizontal plane and a vertical plane to horizontally and vertically manipulate the gripping jaw 30, and the first gripping end is adapted to the test consumable part 22 and the second gripping end is adapted to the sample sucking and blowing mechanism.

Wherein, the clamping jaw can be electronic clamping jaw or pneumatic clamping jaw, and the arm can be a plurality of sharp modules, perhaps adopts the sharp arm of adding the combination of rotation module.

In the embodiment of the present application, the robot arm may be a robot arm as described in 201610081537.4.

In the present embodiment, the gripper 30 is pivotally coupled to the distal end of the robotic arm 20.

In one embodiment, the detection platform further comprises a suction and blowing head detaching piece.

The suction head detaching piece at least comprises a first piece part 51 and a second piece part 52 which are relatively spaced, the distance between the first piece part 51 and the second piece part 52 is smaller than the radial dimension of the suction head 34, the distance between the first piece part 51 and the second piece part 52 is larger than the radial dimension of the suction-blowing driver, and the first piece part 51 and the second piece part 52 are used for applying force to the suction head 34 from the end face of the suction head 34 so as to make the suction head break away from the suction-blowing driver.

When the sample detection program control operating mechanism is used specifically, the suction-blowing driver connected with the suction-blowing head is operated to enter between the first sheet part 51 and the second sheet part 52, and the suction-blowing driver is operated from bottom to top, so that the first sheet part 51 and the second sheet part 52 apply force to the suction-blowing head from the end face of the suction-blowing head 43, and the suction-blowing head is separated from the suction-blowing driver.

In the embodiment of the present application, a collection box is disposed in the sunken space of the detection table, and the first sheet portion 51 and the second sheet portion 52 are disposed at the edge of the table top right above the detection table.

In another embodiment, a push rod mechanism may be disposed on the card seat, the suction/blowing driver placement station, or the suction/blowing driver, the push rod mechanism is configured to be a fixed portion and a telescopic portion inserted into the fixed portion, the first sheet portion and the second sheet portion are disposed on the outer periphery of the telescopic portion, and the first sheet portion and the second sheet portion may be used as a force applying structure to apply a force to the suction/blowing head to separate the suction/blowing head from the suction/blowing driver by the movement of the telescopic portion.

In an embodiment of the present application, the detection consumable part is any one of:

-a test card;

-a cuvette;

-a centrifuge tube;

-cell culture plates.

In the embodiment of the present application, the reading analyzer 50 may be a test card reader, a colorimetry reader, a microplate reader, a molecular amplification instrument, a mass spectrometer, or the like.

The reading analyzer 50 is arranged in a combined manner, and the type and the number of the required devices are arranged according to requirements. The reading instrument can be replaced by different reading instruments according to requirements, and matching of different rapid detection methods is achieved, such as Raman spectroscopy, fluorescence spectroscopy and the like. The commonality is that signal readings can be made for the assay consumables and their internal test samples.

In an embodiment, a bottom plate 60 can be arranged at the bottom of the reading analyzer for fixedly connecting the detection platform and the reading analyzer, the bottom plate is used for connecting the reading analyzer and the reading analyzer without modifying the reading analyzer, and the connection between the reading analyzer and the reading analyzer can be carried out, that is, only the adaptation of different reading analyzers and detection platforms can be realized by transforming the bottom plate 60.

adding a sample to be detected into a sample storage part;

storing the sample storage member in a first storage area of a test station;

storing a detection consumable part corresponding to the sample to be detected in a second storage area of the detection table;

placing the sample suction-blowing mechanism into a placing station of a detection table;

starting a sample detection program corresponding to the sample to be detected;

starting a reading analyzer;

wherein the sample detection procedure comprises:

put into the sample suction and blowing mechanism and examine the station of placing of test table, include:

placing the suction-blowing driver at a placing station of a detection table;

control operating device from placing the station and taking sample suction-blowing mechanism and operating sample suction-blowing mechanism and absorbing the sample that awaits measuring in the sample storage piece of first memory area, include:

In one embodiment, the detection platform further comprises a suction and blowing head detaching piece; the suction and blowing head detaching piece at least comprises a first piece part and a second piece part which are oppositely spaced, the distance between the first piece part and the second piece part is smaller than the radial size of the suction and blowing head, and the distance between the first piece part and the second piece part is larger than the radial size of the suction and blowing driver;

before said controlling said manipulating mechanism to place said sample aspirating and blowing mechanism back into said placing station, further comprising;

In one embodiment, after the controlling the manipulating mechanism takes the detecting consumed material piece from the second storage area and places the detecting consumed material piece in the designated placement area, and before the controlling the manipulating mechanism takes the sample sucking and blowing mechanism from the placement station and manipulates the sample sucking and blowing mechanism to suck the sample to be tested from the sample storage piece in the first storage area, the method further includes:

the control system receives the position information and controls the extension of the telescopic part of the auxiliary positioning piece according to the position information so as to limit the current position of the detection material consumption piece.

The detection method provided by this embodiment further includes:

controlling a handling mechanism to take the detection consumable parts from the second storage area and place the detection consumable parts in a specified placement area;

and controlling the operating mechanism to place the sample suction and blowing mechanism back to the placing station.

Compared with the prior art, the full-automatic detection platform and the detection method provided by the application have the beneficial effects that:

secondly, effectively shorten the waiting period of test, can carry out detection work all weather, shorten production cycle, promote material circulation efficiency.

fourthly, in the operation process of some samples, particularly when testing the microbial correlation items, the phenomenon that the samples and the tests are interfered to obtain wrong results due to the fact that the samples or the clothes have pollution sources occasionally occurs because personnel stay in different environments is caused, and the automatic operation process can effectively reduce and stop the pollution to the samples.

Fifthly, the detection platform provided by the application can be configured with various sensors and camera equipment as required, and based on the visual feedback configuration platform, the process misoperation is prevented through the detection process of the visual feedback auxiliary platform, and the process can be reviewed and analyzed for statistics.

The detection platform that its sixth, this application provided, preorder and the effective linking of subsequence station, no matter be operation or data, reduce the linking cost of process on the whole, realize continuity, the traceability of data, reduce the artificial data loss and the statistics cost of linking process, save data transmission time.

Seventhly, the testing platform that this application provided, peripheral parts such as accessible increase dustcoat, realize environmental control in the minizone and with low costs, like cleanliness factor, temperature humidity, damage nature physical factor (optics, magnetism, radiation etc.) etc. when realizing more accurate testing process, can develop the human intolerable destructive experiment of special requirement, obtain more complete scientific data.

The full-automatic detection platform and the detection method provided by the application can be suitable for food safety detection, such as automatic detection processes of immunochromatography, enzyme-linked immunosorbent assay, colorimetric method, fluorescence method, nucleic acid molecule detection and the like.

The full-automatic detection platform and the detection method provided by the application can be suitable for environment and clinical detection similar to the above processes.

The full-automatic detection platform and the detection method provided by the application can be suitable for pretreatment of detection of food safety instruments and devices, such as mass spectrometry, high performance liquid chromatography, plasma coupling mass spectrometry and other methods, and operations such as liquid adding (dilution or auxiliary reagent adding) and filtering are required before sample loading.

The application takes the immunochromatography test of veterinary drug residues and the spectrophotometry test of pesticide residues as examples for illustration.

1. Performing immunochromatography for testing veterinary drug residues

Milk to be analyzed is added to the sample tube and placed in the sample storage box. And (3) placing the immunochromatographic card to be tested into a storage bin, clicking a screen of a control system, inputting a sample to be tested and card information, and clicking to start. The mechanical arm takes the card and places in the cassette, and position sensor on the cassette discerns the card and arrives the position, and the pars contractilis of fixed auxiliary member shifts out, compresses tightly the card. And then taking the pipette out of the upper part of the gun head box to take the gun head. And immersing the gun head into the sample to absorb the sample to be detected, and adding the sample to the card of the card holder. The pipettor was then placed back into position and the tip discarded. And (4) moving the fixed auxiliary part back, taking the card in the card seat by the mechanical arm, and putting the card into the chromatography reading instrument. And the reading instrument reads, the result is transmitted back to the software, and the screen displays the result and carries out real-time statistics.

2. Performing spectrophotometric test for pesticide residue

Adding the green vegetable pesticide residue extracting solution and the test solution to be analyzed into a sample tube, and placing the sample tube into a sample storage box. The cuvette was placed in a storage bin. Clicking the screen to input the sample and card information to be tested, and clicking to start. The cell is as for the cassette with the arm, and the cell is discerned to the sensor in the cassette and targets in place, and fixed auxiliary member shifts out, will block and support the cell. And taking the pipette up to the upper part of the gun head box to take the gun head. And (4) sucking the sample to be detected from the sample sucked by the gun head, and adding the sample to be detected into the cuvette of the base. And replacing the gun head, sucking the test solution of the test solution, and adding the test solution into the cuvette for uniformly mixing. The pipette is then placed back into position and the tip discarded. And after a specific time, the fixed auxiliary part moves back, the mechanical arm takes the cuvette in the clamping seat and puts the cuvette into the residual pesticide color reading instrument. And reading by a reading instrument, transmitting the result back to software, displaying the result on a screen, and performing real-time statistics.

3. Testing liquid feeding accuracy

Respectively add 150uL water in 50 cuvettes in succession, weigh the cuvettes front and back weight, compare the difference of testing platform automation process and manual operation, as shown in the following table:

4. test card detection value floats

Selecting 15 aflatoxin B1 reagent cards with the detection limit of 0.5ppb, dividing the solution with the standard of 0.5ppb into 30 sample tubes respectively, wherein 15 sample tubes are placed in a sample frame area, placing the cards in a card cabin, and executing a detection program. Likewise, 15 tests were performed manually. The comparison results are shown in the following table:

the above description is only exemplary of the present application and should not be taken as limiting the present application, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A fully automated testing platform, comprising:

the detection platform is at least provided with a first storage area and a second storage area, the first storage area is used for storing the sample storage piece, and the second storage area is used for storing the detection consumable piece;

the sample suction and blowing mechanism is used for sucking a sample to be detected from the inlet and the outlet and blowing the sample to be detected to the detection consumable part;

2. The fully automated testing platform of claim 1, wherein: the sample sucking and blowing mechanism comprises a sucking and blowing driver and a sucking and blowing head which is adapted to the sucking and blowing driver;

3. The fully automated testing platform of claim 2, wherein: the suction-blowing controller is characterized in that a control circuit is arranged in the suction-blowing driver, the control circuit is electrically connected to the control system, and the control system is further used for adjusting the control circuit according to the sample detection program so as to control the output power of the suction-blowing controller.

4. The fully automated testing platform of claim 1, wherein: the testing platform still includes detects the seat, it includes to detect the seat:

the auxiliary positioning part comprises a fixing part and an abutting part which is connected with the fixing part and can move relative to the fixing part, and the abutting part is used for limiting the position of the detection consumable part in the placement area.

5. The fully automated testing platform of claim 1, wherein: the operating mechanism comprises a mechanical arm arranged on the detection table and a clamping jaw connected to the tail end of the mechanical arm, and the clamping jaw is provided with a first clamping end and a second clamping end which are oppositely arranged;

the mechanical arm is used for operating on a horizontal plane and a vertical plane to operate the clamping jaw, the first clamping end is adapted to the detection consumable part, and the second clamping end is adapted to the sample suction and blowing mechanism.

6. The fully automated testing platform of claim 2, wherein: the third storage area is provided with a support assembly;

7. The fully automated testing platform of claim 6, wherein: the supporting component also comprises a supporting plate which is elastically supported on the supporting seat;

be provided with the regulation stand in the backup pad, the regulation through-hole has been seted up on the storage seat, the radial dimension of regulation through-hole is greater than the radial dimension of regulation stand, the storage seat support in the backup pad just adjust the through-hole cup joint in adjust the stand.

8. The fully automated testing platform of claim 1, wherein:

the first storage area is provided with at least one storage box, the storage box is provided with a plurality of storage rooms which are arranged according to a regular array, and the storage rooms are used for storing the sample storage parts;

9. The fully automated testing platform of claim 2, wherein: the detection platform also comprises a suction-blowing head detaching piece;

the suction blowing head detaching piece at least comprises a first piece portion and a second piece portion which are spaced relatively, the distance between the first piece portion and the second piece portion is smaller than the radial size of the suction blowing head, the distance between the first piece portion and the second piece portion is larger than the radial size of the suction blowing driver, and the first piece portion and the second piece portion are used for being separated from the end face of the suction blowing head to the force application of the suction blowing head so as to enable the suction blowing head to be separated from the suction blowing driver.

10. The fully automated testing platform of claim 1, wherein: the detection consumable part is any one of the following parts:

-a test card;

-a cuvette;

-a centrifuge tube;

-cell culture plates.

11. An inspection method corresponding to the fully automated inspection platform of claim 1, comprising:

adding a sample to be detected into a sample storage part;

storing the sample storage member in a first storage area of a test station;

starting a sample detection program corresponding to the sample to be detected;

starting a reading analyzer;

wherein the sample detection procedure comprises:

controlling a handling mechanism to take the detection consumable part from the second storage area and position the detection consumable part at a spatial location;

12. The detection method according to claim 11, characterized in that:

the sample sucking and blowing mechanism comprises a sucking and blowing driver and a sucking and blowing head which is adapted to the sucking and blowing driver;

the detection method further comprises the following steps:

the control of the manipulation mechanism to manipulate the sample suction and blowing mechanism to suck the sample to be tested from the sample storage part in the first storage area comprises the following steps:

controlling the operating mechanism to take the suction-blowing driver and operating the suction-blowing driver to be connected with the suction-blowing head;

13. The detection method according to claim 12, characterized in that:

the control mechanism takes the suction-blowing driver and controls the suction-blowing driver to be connected with the suction-blowing head, and the control mechanism comprises:

and controlling the control mechanism to control the suction-blowing driver from top to bottom and pressing the suction-blowing driver to be sleeved on the suction-blowing head.

14. The detection method according to claim 12, characterized in that:

the detection platform also comprises a suction and blowing head detaching piece; the suction blowing head detaching piece at least comprises a first piece part and a second piece part which are oppositely spaced, the distance between the first piece part and the second piece part is smaller than the radial dimension of the suction blowing head, and the distance between the first piece part and the second piece part is larger than the radial dimension of the suction blowing driver;

before said controlling said handling mechanism to take said test consumable coated with said sample to be tested to said reading analyzer, further comprising;

15. The detection method according to claim 11, characterized in that:

after the control and control mechanism takes the detection consumable part from the second storage area and positions the detection consumable part at a certain spatial position, and before the control and control mechanism takes the sample suction and blowing mechanism and controls the sample suction and blowing mechanism to suck the sample to be tested from the sample storage part in the first storage area, the method further comprises the following steps:

16. The detection method according to claim 11, characterized in that: further comprising: