CN114788443A

CN114788443A - Method for promoting seed germination of hydrangea schreberi at low temperature

Info

Publication number: CN114788443A
Application number: CN202210462558.6A
Authority: CN
Inventors: 刘国宇; 王庆; 李艳; 王莉; 齐康学; 王玮; 刘炼红
Original assignee: XI'AN BOTANICAL GARDEN OF SHAANXI PROVINCE
Current assignee: XI'AN BOTANICAL GARDEN OF SHAANXI PROVINCE
Priority date: 2022-04-28
Filing date: 2022-04-28
Publication date: 2022-07-26
Anticipated expiration: 2042-04-28
Also published as: CN114788443B

Abstract

The invention discloses a method for promoting seed germination of Brasenia schreberi hydrangea at a low temperature, which belongs to the technical field of Brasenia schreberi hydrangea and comprises the following steps: s1: harvesting Brasenia schreberi fruits, drying and stripping seeds; s2: adding water to the capped sterile ep tube; s3: then placing the Brasenia schreberi hydrangea seeds into an ep tube filled with water, tightly covering a cover, and shaking along the length direction of the ep tube to ensure that the seeds are fully contacted with the water; s4: finally, placing the ep tube filled with the seeds under the condition of direct light or scattered light, and shaking the ep tube every 2-3 days to ensure that all the seeds can uniformly contact with water; the ep tube is transparent, so that the requirement of seed germination on light can be met; water does not need to be changed during germination; the air in the gap of the ep tube maintains oxygen required by seed germination, the method can improve the germination rate and the germination speed of the Brasenia schreberi hydrangea at a lower temperature of 5-15 ℃, and the method is simple to operate and low in required cost; solves the problem that seeds are difficult to germinate at a lower temperature in the prior art.

Description

Method for promoting seed germination of hydrangea schreberi at low temperature

Technical Field

The invention relates to the technical field of Brasenia schreberi hydrangea, in particular to a method for promoting seed germination of Brasenia schreberi hydrangea at a low temperature.

Background

Brasenia schreberi hydrangea is also called as long-handled hydrangea, and is an erect shrub of hydrangeaceae hydrangea. Is distributed in Henan, Shaanxi (Daba mountain), south of Gansu, Hubei, Hunan, Sichuan, Guizhou province and other provinces of China. The embroidered balls are various in varieties, bright in color, large in inflorescence and long in flowering period, can be used as ornamental shrubs, fresh cut flowers and potted flowers, and have high ornamental value. The Brasenia schreberi hydrangea is widely distributed in the Qin mountains of Shanxi, and can be developed into local plants applied to landscape architecture.

In the areas of Jiangzhe and southwest of China, hydrangea balls are widely applied to landscape architecture. In recent years, the embroidered balls are increasingly applied to northern areas of China. China is the germplasm resource center of hydrangea, but the market of hydrangea in China is monopolized by imported varieties, and wild hydrangea resources such as Brasenia aquatica are not fully developed and utilized. The flowers of hydrangea are mostly uncultivated flowers, the fruiting rate is low, and natural mature seeds are difficult to obtain. The seeds are very tiny, the average length of the Brasenia schreberi hydrangea seeds is 0.75mm, the width of the Brasenia schreberi hydrangea seeds is 0.29mm, the thousand seed weight is 11.8mg, the harvesting and the sowing are very difficult, and the major problems are that the hydrangea breeding work in China is hindered.

Through a large amount of experiments and reference data, the main reproduction mode of hydrangea is asexual reproduction, including cuttage and tissue culture rapid reproduction technology; meanwhile, the method also relates to the research on the reproduction of hydrangea seeds. At present, the main problems existing in the germination of hydrangea seeds are as follows: (1) the optimal germination temperature of hydrangea seeds is 25 ℃, but the maturation time of the hydrangea seeds is generally 9-12 months, the temperature is too low, and the germination requirement cannot be met under natural conditions; (2) the hydrangea seeds must have certain illumination for germination, and the seeds are directly buried in soil, so that the seeds cannot germinate due to lack of illumination or have extremely low germination rate; (3) the seeds are spread on the soil surface, and a plastic film is required to be covered to keep the humidity of the soil surface, but the seeds are easy to cause plant diseases and insect pests due to improper ventilation. Therefore, a method for promoting germination of seeds of the hydrangea schreberi at a low temperature is urgently needed.

Disclosure of Invention

The invention mainly solves the problem that seeds of Brasenia schreberi hydrangea are difficult to germinate at a lower temperature, and provides the method for germinating Brasenia schreberi seeds, which has the advantages of high germination rate and high germination speed at a lower temperature, prevention of plant diseases and insect pests and simple operation.

In order to achieve the purpose, the invention provides the following technical scheme:

a method for promoting germination of seeds of hydrangea schreberi at low temperature comprises the following steps:

s1: harvesting Brasenia schreberi fruits, drying and stripping seeds;

s2: adding water to the capped sterile ep tube;

s3: then placing the Brasenia schreberi hydrangea seeds into an ep tube filled with water, tightly covering a cover, and shaking along the length direction of the ep tube to ensure that the seeds are fully contacted with the water;

s4: and finally, placing the ep tube filled with the seeds under the condition of direct light or scattered light, and shaking the ep tube every 2-3 days to ensure that all the seeds can uniformly contact with water.

As a further scheme of the invention: in the step S1, full and mature Brasenia schreberi hydrangea fruits are collected in 9-10 months, placed at room temperature, and shaken gently to enable the seeds to naturally fall off after the pods are completely dried.

As a further scheme of the invention: in step S2, a 2mL capped sterile ep tube was selected, and tap water or purified water was filled into the tube to a distance of 0.5cm from the tube opening.

As a further scheme of the invention: in step S4, the ep tube containing the seeds is placed in a 5-15 ℃ light incubator and shaken every 2-3 days to ensure that all seeds are in uniform contact with water.

As a further scheme of the invention: in step S4, the eppendorf tube with the seeds is placed in a 15 ℃ light incubator and shaken every 2-3 days to ensure that all seeds are in uniform contact with the water.

Compared with the prior art, the invention has the beneficial effects that: the ep tube is transparent, so that the requirement of seed germination on light can be met; tap water can stably provide moisture required by seeds, and water does not need to be changed during germination; the method can improve the germination rate and the germination speed of the Brasenia schreberi at a lower temperature of 5-15 ℃, is simple to operate, does not need special instruments and equipment, and has extremely low required cost.

Detailed Description

Example 1

A method for promoting germination of seeds of hydrangea schreberi at a low temperature comprises the following steps:

s1: collecting full and mature Brasenia schreberi fruits in 9 months, standing at room temperature, and shaking gently to make the seeds fall off naturally after the pods are completely dried;

s2: selecting a 2mL aseptic ep tube with a cover, and filling tap water or purified water into the tube to a position 0.5cm away from the tube opening;

s4: the eppendorf tube with the seeds was placed in a 15 ℃ light incubator and shaken every 3 days to ensure that all seeds were in uniform contact with the water.

Example 2

s3: then, placing the Brasenia schreberi hydrangea seeds into an ep tube filled with water, tightly covering a cover, and then shaking along the length direction of the ep tube to ensure that the seeds are fully contacted with the water;

s4: the ep tube with the seeds is placed in a 10 ℃ light incubator and shaken every 3 days to ensure that all seeds are in uniform contact with the water.

Example 3

s1: harvesting full and mature Brasenia schreberi fruits in 9 months, standing at room temperature, and after the pods are completely dried, slightly shaking to enable the seeds to naturally fall off;

s4: the eppendorf tube with the seeds was placed in a 5 ℃ light incubator and shaken every 3 days to ensure that all seeds were in uniform contact with the water.

Comparative example 1

Placing the Brasenia schreberi seeds in a culture dish, and then placing the culture dish in a 15 ℃ illumination incubator for culture.

Comparative example 2

Placing Brasenia schreberi Maxim seeds in a culture dish, and then placing the culture dish in a 10 ℃ illumination incubator for culture.

Comparative example 3

Placing the Brasenia schreberi seeds in a culture dish, and then placing the culture dish in a lighting incubator at 5 ℃ for culture.

The results of the experimental data obtained in the above examples and comparative examples are shown in table 1.

TABLE 1

As can be seen from the data in Table 1, the ep tube can meet the light requirements of seed germination, the germination rate of the seeds in the ep tube is the highest at 15 ℃, the germination time is the shortest, and the germination rate of the seeds in the ep tube is higher than that of the seeds in a culture dish, and the germination time is short.

In conclusion: the ep tube is transparent, so that the requirement of seed germination on light can be met; tap water can stably provide water required by the seeds, and water does not need to be changed during germination; the method can improve the germination rate and the germination speed of the Brasenia schreberi at a lower temperature of 5-15 ℃, is simple to operate, does not need special instruments and equipment, and has extremely low required cost.

The present invention is not limited to the above-described embodiments, and various changes or modifications to the present invention are intended to be included within the scope of the claims and the equivalent technology of the present invention, if they do not depart from the spirit and scope of the present invention.

Claims

1. A method for promoting germination of seeds of hydrangea schreberi at a low temperature is characterized by comprising the following steps:

s1: harvesting Brasenia schreberi fruit, drying, and removing seeds;

s2: adding water to the capped sterile ep tube;

2. The method of claim 1, wherein in step S1, the mature and full Brasenia schreberi fruits are harvested at 9-10 months, left at room temperature, and shaken to make the seeds fall off naturally after the pods are completely dried.

3. The method of claim 1, wherein in step S2, a 2mL aseptic ep tube with a cover is selected, and tap water or purified water is filled into the ep tube to a distance of 0.5cm from the tube opening.

4. The method of claim 1, wherein in step S4, the eptube containing the seeds is placed in an incubator at 5-15 ℃ and shaken every 2-3 days to ensure that all seeds are uniformly exposed to water.

5. The method for promoting germination of seeds of hydrangea lactuca under low temperature according to claim 4, wherein in step S4, the ep tube containing seeds is placed in a 15 ℃ illumination incubator, and the ep tube is shaken every 2-3 days to ensure that all seeds are uniformly contacted with water.