CN114774438B - Osmanthus gene OfTPS380.1 and application thereof - Google Patents
Osmanthus gene OfTPS380.1 and application thereof Download PDFInfo
- Publication number
- CN114774438B CN114774438B CN202210420147.0A CN202210420147A CN114774438B CN 114774438 B CN114774438 B CN 114774438B CN 202210420147 A CN202210420147 A CN 202210420147A CN 114774438 B CN114774438 B CN 114774438B
- Authority
- CN
- China
- Prior art keywords
- gene
- geraniol
- osmanthus
- saccharomyces cerevisiae
- expression vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/07—Diphosphoric monoester hydrolases (3.1.7)
- C12Y301/07011—Geranyl diphosphate diphosphatase (3.1.7.11)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of molecular biology and discloses an osmanthus geneOfTPS380.1And the application thereof, through genome data bioinformatics analysis, the high-expression geraniol synthase gene in osmanthus flower petals is screenedOfTPS380.1，OfTPS380.1The full-length sequence of the gene CDS is shown as SEQ ID NO.1, and the gene CDS contains the geneOfTPS380.1The expression vector of the (2) is used for transforming the saccharomyces cerevisiae and fermenting and culturing, the geraniol yield reaches 21.67mg/L, and the method has the value of industrial production.
Description
Technical Field
The invention belongs to the technical field of molecular biology, and in particular relates to an osmanthus gene OfTPS380.1 and application thereof in geraniol production
Background
Geraniol is an acyclic monoterpene alcohol compound extracted from plants. The rose flower liquid is colorless to yellow oily liquid at normal temperature, has mild and sweet rose flower smell, and has bitter taste. It is one of the main components of essential oils such as rose oil, martin oil and citronella oil, and is also present in small amounts in geranium and lemon. Geraniol and geranyl alcohol esters are widely used as daily essences and edible essences, are main agents of rose essences, and are used for preparing daily products and foods. Meanwhile, the geraniol is used for antibiosis and expelling parasites, has good clinical treatment effect on chronic bronchitis, can improve the lung ventilation function and reduce the airway resistance, and is also beneficial to improving the body immunity.
The osmanthus fragrans is a famous fragrant flower plant and contains abundant terpenoid substances, so that the osmanthus fragrans not only can bring pleasant mental feeling, but also has health care values of sterilization, anti-inflammation, antioxidation, anti-aging and the like. The genome of the osmanthus fragrans contains a large number of fragrance genes, but the related reports of geraniol synthase genes in the osmanthus fragrans are not yet available. The invention utilizes bioinformatics technology to screen geraniol synthase genes in osmanthus fragrans and performs functional verification in saccharomyces cerevisiae.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention screens the high-expression geraniol synthase gene OfTPS380.1 in osmanthus petals from the osmanthus fragrans genome and performs functional verification in saccharomyces cerevisiae.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
osmanthus fragrans geraniol synthase gene OfTPS380.1: through genome data bioinformatics analysis, terpene genes OfTPS380.1 which are highly expressed in osmanthus flower petals are screened, and CDS sequences of the terpene genes are shown as SEQ ID NO. 1.
An expression vector comprising the geraniol synthase gene oftps380.1.
Recombinant saccharomyces cerevisiae expressing OfTPS380.1 gene and application thereof: transforming a yeast expression vector containing the gene OfTPS380.1 into Saccharomyces cerevisiae, and obtaining recombinant Saccharomyces cerevisiae for expressing the gene OfTPS380.1 through a resistance plate and sequencing and screening; inoculating recombinant Saccharomyces cerevisiae into YPD culture medium, adding isopropyl myristate for covering, and extracting geraniol after fermentation.
Compared with the prior art, the invention has the following advantages:
the geraniol synthase gene is seldom researched in plants, the geraniol synthase gene is excavated in the osmanthus fragrans for the first time, and the function verification is carried out in the saccharomyces cerevisiae, so that the geraniol yield reaches 21.67mg/L, and the geraniol synthase gene has the value of industrial production.
Drawings
FIG. 1 is a clone gel map of OfTPS380.1 in example 1
FIG. 2 is a map of expression vector plasmids in example 2.
FIG. 3 shows the GC-MS detection results of geraniol.
FIG. 4 is a fragment diagram of geraniol ions.
Detailed Description
The following description of the embodiments of the present invention is provided by way of illustration only and not by way of limitation, with reference to the accompanying drawings and examples.
Example 1 Gene screening and cloning
Gene screening and cloning: through genome data bioinformatics analysis, a geraniol synthase gene (OfTPS 380.1) which is highly expressed in osmanthus flower petals is screened, primer5.0 software is used for designing specific primers OfTPS380.1-CDS-F and OfTPS380.1-CDS-R for amplifying the full length of CDS of the OfTPS380.1 gene, and Beijing family biotechnology limited company is used for synthesizing amplification primers. The target gene CDS is amplified by taking osmanthus fragrans cDNA as a template and referring to the description of the Phanta high-fidelity enzyme, and the PCR reaction system and the PCR reaction program are shown in tables 2 and 3.
TABLE 1 primer list
TABLE 2PCR reaction System
TABLE 3PCR reaction procedure
Gel electrophoresis detection and gene sequencing: 30ml of TAE was added with 0.45g of agarose powder, and after boiling in a microwave oven, 3. Mu.l of 10000 Xnucleic acid dye was added, and the mixture was poured into a gel plate, and after solidification, spotting was performed. After electrophoresis at 120V and 150mA for 30min, the bands were visualized on a Gel-Logie200 Gel scanning imager (as shown in FIG. 1). And (3) connecting a target strip with a T-carrier after recovery, converting escherichia coli, sequencing, wherein the sequence of the OfTPS380.1 gene CDS is shown as SEQ ID NO.1, extracting plasmids from bacterial liquid with correct sequencing, and naming the plasmids as OfTPS380.1-CDS-Plasmid as templates for constructing the carrier later.
EXAMPLE 2 vector construction
Construction of a yeast carrier: the OfTPS380.1-CDS-Plasmid is used as a template, an upstream primer OfTPS380.1-BsaI-F/R (table 1) carrying BsaI enzyme cutting sites is designed to clone the OfTPS380.1 gene fragment, and the obtained PCR product and an expression vector Plasmid (a Plasmid map is shown in figure 2 and presented by a Liu Tian teacher subject group) to be constructed are subjected to Goldengate connection. The PCR reaction system and the procedure are shown in Table 2 and Table 3, the product is directly transformed by colibacillus, monoclonal positive detection is selected, positive single colony shaking bacteria is carried out to extract plasmid, enzyme digestion is verified, the enzyme digestion is verified to correct plasmid sequencing, and the plasmid with correct sequencing is named as: ofTPS380.1-Pkz762-Plasmid for subsequent transformation of Saccharomyces cerevisiae.
Example 3 Yeast transformation and plate screening
Preparation of YPD solid medium: 2% peptone, 1% yeast extract, 10g/L agar, adding distilled water, heating to dissolve, and fixing volume to 0.9 times of final volume; sterilizing at 115 deg.C for 30min, and adding 0.1 times of 20% glucose solution to obtain YPD medium. SC-URA screening Medium: the preparation method comprises the steps of weighing an amino acid mixture of uracil deficiency in a yeast nitrogen source basic culture medium YNB with the concentration of 0.67%, adjusting the pH to 6.5 by using a 2M NaOH solution, adding distilled water, heating and dissolving, fixing the volume to 0.9 times of the volume, and sterilizing for 30min at 115 ℃; after sterilization, 0.1 times the volume of 20% dextrose solution was added.
Table 4 amino acid mixture formulation
10×te configuration: 100mM Tris and 10mM EDTA, pH adjusted to 7.5 with hydrochloric acid, and finally autoclaved at 115℃for 30min, and stored at room temperature.
10×liac (lithium acetate) configuration: 1M LiAc, pH was adjusted to 7.5 with glacial acetic acid (acetic acid), then sterilized with a 0.22. Mu.M sterile filter membrane, and placed in a refrigerator at 4deg.C for use.
50% PEG4000: weighing 40g of PEG4000, adding a proper amount of water, heating until the PEG4000 is completely dissolved, and adding water to a constant volume of 80mL; sterilizing at 115deg.C for 30min, packaging 600 μl/tube, and placing in a refrigerator at 4deg.C.
DMSO: sterile 0.22 μm filter membrane was sterilized and placed in a refrigerator at 4 ℃.
Single-stranded fish sperm DNA (SSDNA): purchased from beijing cool labs science and technology limited.
The saccharomyces cerevisiae strain YZL141 transformation and plate screening method is specifically as follows:
1. selecting about 5 Saccharomyces cerevisiae YZL141 monoclone strains, placing the monoclone strains in a PA bottle containing 5ml YPD liquid culture medium, and culturing the monoclone strains overnight at 30 ℃; 50 μl of the bacterial liquid was diluted 20 times with sterile water, and OD was measured 600 Values (20-fold dilution, not to make OD values greater than 1, otherwise inaccurate); according to OD 600 The formula ×volume=10 (e.g. if od=2, 5ml, typically between 500-1000 μl) is transferred to 50ml fresh YPD medium and incubated at 30 ℃,220rpm for 3.5h; when the OD600 is 0.5-0.8, centrifuging at 3000rpm for 5min to collect thalli; adding 20ml of 1 xTE solution to resuspend cells, centrifuging at 3000rpm for 5min, and discarding the supernatant; to the precipitated cells, 2ml of 1 XLiAc/0.5 XSTE (1700. Mu. l H) was added 2 O+200. Mu.l of 10 XLIAc+100. Mu.l of 10 XTE) and left at room temperature for 10min (preferably 25 ℃);
2. the single-stranded fish sperm DNA is boiled for 10min and then quickly put back on ice for cooling, so that the fish sperm DNA is ensured to be in a single-stranded state, 100 mu l of yeast strain YZL141 suspension is taken, 10 mu l of denatured single-stranded fish sperm DNA is added, 300ng of OfTPS380.1-Pkz762-Plasmid which is diluted in advance for standby is added, light and elastic mixing is carried out, and finally 700 mu l of 1 xLiAc/40% PEG4000/1 xTE solution (560 mu l of PEG4000+70 mu l of 10 xLiAc+70 mu l of 10 xTE) is added and mixed uniformly; culturing at 30deg.C for 30min; adding 88 μl of DMSO, mixing, and heat-shock for 9min at 42 ℃; centrifuging at 10000rpm for 30s, and discarding supernatant; adding 1ml YPD culture medium to resuspend thalli, centrifuging at 8000rpm for 30s, and discarding the supernatant; adding 1ml YPD culture medium to resuspend the thallus, and incubating for 30min at 30 ℃ in a shaking table; centrifuging at 10000rpm for 30s, and discarding supernatant; adding 1ml of 1 xTE solution to resuspend the thalli, centrifuging at 10000rpm for 30s, and discarding the supernatant;
3. the transformed YZL141 is coated on an SC-URA screening plate, and after the liquid is dried, the plate is placed in a 30 ℃ incubator for culturing, and obvious single colony is visible about 2-3 days.
EXAMPLE 4 Yeast transformed colony Positive detection
Single colonies of transformed yeasts were picked up into 10. Mu.l of sterile water, 2. Mu.l were removed, 10. Mu.l of 20mM NaOH solution was added, treated with a PCR apparatus at 99℃for 20min, placed on ice for template, and vortexed for 2s to be mixed well before use to prevent sedimentation.
PCR System (10 μl): 2 XTaq Master Mix 5. Mu.l, ofTPS380.1-Yeast transformation Positive detection-F/R0.5. Mu.l each, template 1. Mu.l, water 3. Mu.l, PCR conditions were: pre-denaturation at 94℃for 2min, denaturation at 94℃for 20S, annealing at 55℃for 20S, extension at 72℃for 1min/kb, 30 cycles were set and final extension at 72℃for 5min. After electrophoresis of the PCR products, bands were observed, and if there were obvious positive bands (1752 bp of the target band), yeast fermentation and geraniol GC-MS detection could be performed.
Example 5 Yeast fermentation and geraniol GC-MS detection
1. Shaking flask fermentation culture
YPD liquid medium configuration: the final concentration of peptone (Angel brand) was 20g/L, the final concentration of yeast powder (Angel brand) was 10g/L, the final concentration of glucose was 10g/L, the final concentration of galactose was 10g/L (glucose and galactose were separately prepared, sterilized and then added to the medium), and isopropyl myristate (IPM) was filtered with a sterile filtration membrane before use.
Inoculating: from the transformed plates, 5 single colonies that detected positive were picked and incubated in 5ml YPD liquid medium at 220rpm at 30℃overnight. The culture broth is sucked overnight the next day, added into 50ml liquid culture medium according to a certain proportion to make the initial OD 600 Has a value of about 0.1, andcovering with 10% isopropyl myristate, and culturing at 30deg.C under shaking conditions of 220rpm for 72 hr.
GC-MS quantitative detection of geraniol
After the fermentation, 200. Mu.l of isopropyl myristate was added to the sample bottle after centrifugation at 4000rpm at 4℃for 10 minutes, and the product was detected by GC-MS, while 1. Mu.l of methyl nonanoate at a concentration of 4.75mg/L was added as an internal standard for quantitative calculation, wherein the untransformed Saccharomyces cerevisiae strain YZL141 was used as a negative control, and the quantitative calculation formula: c1/v1=c2/V2 (C1 is the peak area of methyl nonanoate, V1 is the concentration of methyl nonanoate, C2 is the peak area of geraniol, V2 is the concentration of geraniol).
GC-MS detection:
GC settings: the temperature of the sample inlet is 250 ℃, the sample injection mode is not split, the initial temperature of a heating program is 40 ℃, the temperature is kept at 3.5min, the heating rate of 10 ℃/min is increased to 100 ℃, the temperature is kept for 3min, and then the temperature is increased to 280 ℃ at the rate of 5 ℃/min, and the temperature is kept for 5min. The total program time was 46.5min.
Setting up MS: the interface temperature is 280 ℃, the loading gas is helium, the flow rate is 1.0ml/min, the ion source temperature is 220 ℃, the EI ionization mode is adopted, the electron energy is 70ev, and the scanning range is 50-500amu.
The sample injection mode is a non-split mode, the temperature of the sample injection port is maintained at 230 ℃, and the temperature of the transmission line is 240 ℃. The electron energy is 70eV, the scanning range is 40-450amu, the ion source temperature is 150 ℃, high-purity helium (99.999%) is used as carrier gas, and the flow rate is 0.8mL/min. The initial column temperature was 40℃for 3min, then at a rate of 1℃per minute to 80℃for 3min, then at a rate of 10℃per minute to 220℃for 15min.
The GC-MS detection shows that the peak of the target product is geraniol, the peak time of the target is 7.58min, and the final concentration of geraniol is 21.67mg/L.
Sequence listing
<110> university of agriculture in China
High and new osmanthus fragrans industry technical institute in Mining market
<120> Osmanthus gene OfTPS380.1 and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1752
<212> DNA
<213> Artificial sequence (Artificial Sequence)
<400> 1
atggagtgta caatgaggag tatttcctta ttttcacagt catctaatgg tatttcagga 60
actgcaagaa gtccatttca atggccgata aaccatcgat tttcatcggg tcaacgtgat 120
ttcgtttgca agtcgttgcc ggtgtcgttg cctagtgtaa cgccattgat tcctgctgaa 180
aatgatgctc tgtataatta tatacgccaa cctgtgatag ttactcccga agtcgatgag 240
ggcgcgaagc acagagaatt gctcgaaaaa actcgacgag aactgcaaag aagtacaaaa 300
ccagttgaga cgctaaaact tatagacaaa ctccaacgat taggaattgc gtattatttt 360
gaggatgata tcaatgcaat actggatcga ttctctgatg gcttgcctaa tgaagatctc 420
ttcacaacag ccttatgctt ccgcttgctc cgtgataacg gctacaaaac tgattctgat 480
gtctttctta aattcatgga aaagaacaag aaattcaaag aacatttggc tcaagacacc 540
ataggcttat tgagcttata cgaagcatcg tacatgggag caaacggcga agaaatattg 600
tcagaggcca aggaatttac tgaaattcac cttagacagt cgatgcctcg gttggctctg 660
caacttcgtc gacaagttgg ttctgcctta gagctcccga ggcacctccg gatggctagg 720
ttagaagcta ggcgttacat tgaagaatat gctacagaaa gtgaacacga tccagccctt 780
ttggaactgg caagattaga ttataacaaa gtccagttac aacaccaaat ggaattgtct 840
gaaatatcaa gatggtggaa acaattgggg cttgttgaga agctgagctt tgcccgggac 900
agacccttgg aatgcttttt atggactgtg ggacttcttc cagaacccaa atactctagc 960
tgcagaattg aactggctaa gaccatagcc attctattgg taattgacga tatttttgac 1020
acatatggca aaatggagga acttgttctt ttcactgaag caattcaaag atgggatctt 1080
gatgaattgg aaactcttcc accatacatg agaatatgtt acatggcatt atacaacact 1140
accaatgaaa tctgctacaa aatcctcaag gagtatggat tttgtgtcct tccctacctt 1200
aaatccacgt ggatagatat gattgagggg tttatggtag aggcgaattg gtttaatggt 1260
ggacatggac caaatttgga ggaatatata gagaatggtg tttcaacagc aggagcatat 1320
atggctttgg tgcacctatt ctttcttata ggggaaggag ttacaaatga gaatattact 1380
aaattgttga gaaaaccata tcctaaactc ttttccacag ctgggagaat tcttcgtctc 1440
tgggatgatc taggaactgc aaaggaggag gaagaacgag gcgatcttgc atcgtgcatg 1500
cagatattaa tgagagagaa gaacatagat tgtgaaaacg aaggcagaaa atatattctg 1560
aaagctataa atagcctatg gaaagatctg aacgatgaac tgatttcacc aaacgcaatg 1620
ccattagcca ttaccaaagt tgcattgaac atggctagag catccgaagt tgtctacaag 1680
catgaagagg attcatactt ctccagcgtc gataattatg tgcaggcttt gttcttcact 1740
cctattaatt ga 1752
Claims (5)
1. Osmanthus fragrans geraniol synthase geneOfTPS380.1Characterized in that the geneOfTPS380.1The nucleotide sequence of (2) is shown as SEQ ID NO. 1.
2. An expression vector comprising the osmanthus fragrans geraniol synthase gene according to claim 1OfTPS380.1。
3. A recombinant Saccharomyces cerevisiae, which comprises a geneOfTPS380.1Saccharomyces cerevisiae transformed with the yeast expression vector of (2), and expression obtained by resistance plate and sequencing screeningOfTPS380.1Recombinant Saccharomyces cerevisiae of genes, said genesOfTPS380.1The nucleotide sequence of (2) is shown as SEQ ID NO. 1.
4. The osmanthus fragrans geraniol synthase gene of claim 1OfTPS380.1Or the expression vector of claim 2 or the recombinant s.cerevisiae of claim 3 in the synthesis of geraniol.
5. A method for synthesizing geraniol by yeast, comprising the steps of: the recombinant Saccharomyces cerevisiae of claim 3 is inoculated in a galactose-containing YPD medium, and covered with isopropyl myristate, and geraniol is extracted after fermentation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210420147.0A CN114774438B (en) | 2022-04-20 | 2022-04-20 | Osmanthus gene OfTPS380.1 and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210420147.0A CN114774438B (en) | 2022-04-20 | 2022-04-20 | Osmanthus gene OfTPS380.1 and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114774438A CN114774438A (en) | 2022-07-22 |
| CN114774438B true CN114774438B (en) | 2023-04-28 |
Family
ID=82432065
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210420147.0A Active CN114774438B (en) | 2022-04-20 | 2022-04-20 | Osmanthus gene OfTPS380.1 and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114774438B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102344915A (en) * | 2011-09-16 | 2012-02-08 | 中国科学院研究生院 | Protein with cinnamyl alcohol dehydrogenase activity and coding gene as well as application thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4123718B2 (en) * | 2000-12-28 | 2008-07-23 | トヨタ自動車株式会社 | Process for producing geranylgeraniol and its related compounds |
| CN111286482A (en) * | 2020-05-13 | 2020-06-16 | 中国科学院烟台海岸带研究所 | Escherichia coli engineering bacterium capable of rapidly producing geraniol and construction method and application thereof |
| CN113025594B (en) * | 2021-03-04 | 2022-05-31 | 安徽农业大学 | Polypeptide, nucleic acid and application of polypeptide and nucleic acid in synthesis of geraniol |
| CN113774079A (en) * | 2021-08-13 | 2021-12-10 | 中国科学院天津工业生物技术研究所 | Recombinant saccharomyces cerevisiae and construction method and application thereof |
-
2022
- 2022-04-20 CN CN202210420147.0A patent/CN114774438B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102344915A (en) * | 2011-09-16 | 2012-02-08 | 中国科学院研究生院 | Protein with cinnamyl alcohol dehydrogenase activity and coding gene as well as application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114774438A (en) | 2022-07-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101731568B (en) | Method for preparing high-salt dilute soy by adopting immobilized cell fermentation | |
| WO2020134687A1 (en) | Method for preparing ergothioneine by biosynthesis and fermentation medium | |
| CN106978465B (en) | Fermentation method for improving fermentation yield of total triterpenoids in inonotus obliquus | |
| WO2023143136A1 (en) | YEAST ENGINEERING BACTERIUM FOR FERMENTATIVE PRODUCTION OF α-SANTALENE AND USE THEREOF | |
| CN101983240B (en) | Flocculent yeast and method for production thereof | |
| CN105420135A (en) | Recombined saccharomyces cerevisiae bacterial strain of high-yield monoterpene geraniol and application of recombined saccharomyces cerevisiae bacterial strain | |
| CN112175848A (en) | Patchouli alcohol production yeast strain and construction method and application thereof | |
| CN101451110A (en) | Construction method of genetic engineering bacterial strain capable of producing low diacetyl beer | |
| CN110564580A (en) | Method for producing vinegar containing pyrroloquinoline quinone through microbial co-culture fermentation | |
| CN112391418B (en) | Industrialized fermentation production method of raspberry ketone | |
| CN114774438B (en) | Osmanthus gene OfTPS380.1 and application thereof | |
| CN110747138B (en) | Saccharomyces cerevisiae gene engineering bacterium and construction method and application thereof | |
| CN114736918B (en) | Recombinant escherichia coli for producing salidroside by integrated expression and application thereof | |
| CN114438109B (en) | Osmanthus gene OfTPS13.2 and application thereof | |
| CN110004099B (en) | Fermentation production method of salidroside | |
| CN115948265A (en) | Kluyveromyces marxianus haploid yeast and construction method and application thereof | |
| CN105200102B (en) | The method of glutathione is extracted from candida utili fermentation liquid | |
| CN109576287B (en) | CrpMApplication of gene in improving DHA stress tolerance of Gluconobacter strains | |
| CN110283855B (en) | Method for improving GABA (Gamma amino acid butyric acid) producing capability of streptococcus thermophilus 210202 strain | |
| CN110257447B (en) | Method for improving GABA (gamma-aminobutyric acid) production capacity of lactococcus lactis subsp | |
| CN104630188A (en) | Method for producing low-temperature glucomannanase by fermentation of marine microorganisms | |
| CN111893107A (en) | Pichia pastoris engineering strain for heterologous expression of cellulase gene EG IV and application | |
| CN110408546A (en) | A kind of Trichoderma viride solid fermentation process | |
| CN116286513B (en) | Lactobacillus johnsonii FR-1012 and method for industrially producing gamma-aminobutyric acid by same | |
| CN113684160B (en) | Klebsiella bacteria for modifying Rcs signal system and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |