CN114768373A - Automatic concentration system capable of expanding multistage filtration and use method thereof - Google Patents
Automatic concentration system capable of expanding multistage filtration and use method thereof Download PDFInfo
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- CN114768373A CN114768373A CN202210535631.8A CN202210535631A CN114768373A CN 114768373 A CN114768373 A CN 114768373A CN 202210535631 A CN202210535631 A CN 202210535631A CN 114768373 A CN114768373 A CN 114768373A
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Abstract
The invention relates to the technical field of cell concentration, in particular to an automatic concentration system capable of expanding multi-stage filtration and a using method thereof, and solves the problems that a concentration mode in the prior art is long in time consumption and poor in repeatability, the treatment capacity is large, and the whole process is complex. An automatic concentration system capable of expanding multistage filtration and a using method thereof comprise a host module, a filtration module and a harvesting module, wherein the filtration module is provided with a plurality of filtration modules, and the filtration modules can be connected in series or in parallel. The invention can adopt the mode of random combination of series connection, parallel connection or series-parallel connection to achieve the effect required by different application occasions, can cover and meet most requirements of filtration, concentration and purification by different connection modes, has short time consumption and high efficiency, and can effectively control the consistency and the repeatability within the required range.
Description
Technical Field
The invention relates to the technical field of cell concentration, in particular to an automatic concentration system capable of expanding multi-stage filtration and a using method thereof.
Background
The cells cultured in vitro are derived from human or animal body or embryonic tissue, the cells in the body are all mixed and grow, each tissue has blood vessel and mesenchymal tissue, therefore, most of the primary cells and the subculture cells derived from the above-mentioned tissue culture materials grow in a mixed manner, not only has epithelial-like cells, but also has fiber-like cells, and the fiber-like cells include fibroblasts, muscle cells, bone cells, synovial cells, and the like, and the mixed cells can directly influence the experimental results, when in-vitro cultured cells are used for experimental research, in order to ensure the reliability, consistency, stability and repeatability of experimental results, a single type of cells is required to be used for experiments, so that a series of researches on the changes of functions, forms and the like of a certain cell can be carried out, therefore, the purification of cultured cells becomes an important step of experimental research, namely, the process of purifying or concentrating cells.
In the prior art, technical modes such as centrifugation and magnetic beads are generally adopted for cell purification or concentration, the traditional concentration mode is long in time consumption and poor in repeatability, meanwhile, the treatment capacity is also greatly insufficient, the whole process is complex, a hollow fiber column is adopted, although the technology is advanced, the mode and the filtration stage number are single or fixed, the method is not suitable for the requirements of purification or concentration of diversification, multiple varieties and multiple varieties at present, and the inconvenience is very brought.
Disclosure of Invention
The invention aims to provide an automatic concentration system capable of expanding multistage filtration and a using method thereof, and solves the problems that the concentration mode in the prior art is long in time consumption and poor in repeatability, the treatment capacity is large, and the whole process is complex.
In order to achieve the purpose, the invention adopts the following technical scheme:
an automatic concentration system capable of expanding multistage filtration and a using method thereof comprise a host module, a plurality of filtration modules and a harvesting module, wherein the filtration modules are arranged, and the filtration modules can be connected in series or in parallel;
when the filtering modules are connected in series, the operation steps comprise the following points:
the method comprises the following steps: the main machine module is communicated with one of the plurality of filtering modules, adjacent two of the plurality of filtering modules are communicated, and one of the plurality of filtering modules far away from the main machine module is communicated with the harvesting module;
step two: communicating the host module with stock solution, communicating cleaning solution with the host module, pumping the cleaning solution into the stock solution, communicating the stock solution with the filtering module, and communicating a transfer bottle with one side of the filtering module, which is far away from one of the host modules;
step three: the outlet of the transfer bottle is communicated with the harvesting module, and the outlet of the harvesting module is communicated with the harvesting bottle;
step four: an outlet at one end of the cleaning liquid equipment is communicated with the harvesting module.
When the filtering modules are connected in parallel, all the filtering modules are communicated with the host module and the stock solution.
Preferably, the host module comprises a peristaltic pump and stock solution, an inlet of the peristaltic pump is communicated with a two-way connector, an outlet of the peristaltic pump is communicated with a three-way connector, two outlets of the three-way connector are communicated with pinch valves, two outlets of the two pinch valves are communicated with two-way connectors, an outlet of one of the two-way connectors is communicated with the inlet of the stock solution, a filtering membrane is mounted on one side of the stock solution, and an outlet of the stock solution is communicated with the two-way connector;
three of the five two-way joints are sequentially in butt joint with the last-stage filtering module, the cleaning solution, the waste liquid bottle and the other two-way joints.
Preferably, the filtering module comprises a peristaltic pump, a filtering membrane, a transit bottle and a hollow fiber column, wherein the inlet of the peristaltic pump is communicated with a two-way joint which is communicated with the hollow fiber column, two outlets of the hollow fiber column are both communicated with a pinch valve, the outlet of one of the two pinch valves is communicated with a three-way joint, one outlet of the three-way joint is communicated with the pinch valve, the other outlet of the three-way joint is communicated with the transit bottle, the outlet of the pinch valve is communicated with the three-way joint, one outlet of the three-way joint is communicated with the two-way joint, the other outlet of the three-way joint is communicated with the pinch valve, and one side of the transit bottle is provided with the filtering membrane;
two of the six two-way joints are connected to the host module or the filtering module at the previous stage, one two-way joint close to the hollow fiber column is connected to the waste liquid bottle, one two-way joint close to the transfer bottle and the two-way joint on the three-way joint far away from the transfer bottle are communicated with the filtering module at the next stage, and the other two-way joints are in butt joint.
Preferably, the harvesting module comprises two peristaltic pumps, two filtering membranes and a hollow fiber column, an outlet of one of the two peristaltic pumps is communicated with the hollow fiber column, an inlet of the other peristaltic pump is communicated with the hollow fiber column, an outlet of the other peristaltic pump is communicated with the harvesting bottle, an inlet of the peristaltic pump at the inlet of the hollow fiber column is communicated with a two-way joint, the two-way joint is communicated with the last-stage filtering module, an outlet of the hollow fiber column is communicated with a three-way joint, an inlet of the three-way joint is communicated with the two-way joint through a pinch valve, the two-way joint is communicated with the cleaning liquid, the filtering membranes are mounted at the other outlet of the three-way joint and one side of the harvesting bottle, an outlet of the hollow fiber column, far away from the three-way joint, is communicated with the two-way joint through the pinch valve, and the two-way joint is communicated with the waste liquid bottle.
Preferably, the use method of the automatic concentration system with expandable multi-stage filtration comprises the following steps:
the method comprises the following steps: the host module pumps the cleaning solution into the stock solution, and the filtering module pumps the stock solution mixed with the cleaning solution into a transfer bottle through a hollow fiber column in the filtering module to obtain filtrate;
step two: the harvesting module pumps the filtrate in the transfer bottle into a hollow fiber column in the harvesting module, the concentrated solution is left in the hollow fiber column, and the waste liquid is discharged to a waste liquid bottle;
step three: the harvesting module pumps the concentrated solution in the hollow fiber column back to a harvesting bottle.
Preferably, the filtration modules are arranged in a plurality and are communicated with the host module, the filtration modules are subjected to one-to-one filtration in the first step, when the filtration modules are connected in series, the filtration is carried out through hollow fiber columns in the filtration modules, and finally the filtration modules are pumped into one harvesting module through the second step.
The invention has at least the following beneficial effects:
the invention adopts the current more advanced hollow fiber column, breaks the limitation of single or fixed filtration stage number, and is suitable for various occasions needing purification or concentration, wherein, a host module is added with a filtration module and a receiving module, when multistage filtration is needed, the scheme can be set as that the host module is added with a plurality of filtration modules and the receiving module, and a plurality of filtration modules can adopt the mode of series connection, parallel connection or the mode of random combination of series connection and parallel connection to achieve the effect required by different application occasions.
The invention also has the following beneficial effects:
the hollow fiber column can select corresponding specifications according to different requirements so as to achieve the effects of filtration and concentration, meanwhile, containers used for containing cleaning liquid, waste liquid, transfer liquid and the like can be bottled or bagged, the purification or concentration is not limited to the purification or concentration of cells, and the hollow fiber column can be used for other purposes, and only the corresponding hollow fiber column filtration part and the corresponding harvesting part need to be selected and replaced.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a system diagram of a host module of the present invention;
FIG. 2 is a system diagram of a filtration module of the present invention;
FIG. 3 is a system diagram of a harvesting module of the present invention;
FIG. 4 is a flow diagram of the multi-stage filtration of the present invention;
FIG. 5 is a simplified filtration flow diagram of the present invention.
In the figure: 1. a two-way joint; 2. a pinch valve; 3. a peristaltic pump; 4. a hollow fiber column; 5. a three-way joint; 6. a filtration membrane; 7. harvesting the bottles; 8. transferring the bottle; 9. a harvesting module; 10. a filtration module; 11. a host module; 12. and (4) stock solution.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the invention.
Example one
Referring to fig. 1-5, the device comprises a host module 11, a plurality of filtering modules 10 and a harvesting module 9, wherein the filtering modules 10 are provided, and the plurality of filtering modules 10 can be connected in series or in parallel;
when the filter modules 10 are connected in series, the operation steps include the following points:
the method comprises the following steps: the host module 11 is communicated with one of the plurality of filtering modules 10, adjacent two of the plurality of filtering modules 10 are communicated with each other, and one of the plurality of filtering modules 10 far away from the host module 11 is communicated with the harvesting module 9;
step two: the host module 11 is communicated with stock solution 12, cleaning solution is communicated with the host module 11, the cleaning solution is pumped into the stock solution 12, the stock solution 12 is communicated with the filtering modules 10, and one side of one of the filtering modules 10, which is far away from one of the host modules 11, is communicated with a transfer bottle 8;
step three: the outlet of the transfer bottle 8 is communicated with the harvesting module 9, and the outlet of the harvesting module 9 is communicated with the harvesting bottle 7;
step four: an outlet at one end of the cleaning liquid equipment is communicated with the harvesting module 9.
When the filter modules 10 are connected in parallel, all the filter modules 10 are communicated with the host module 11 and the stock solution 12.
The host module 11 comprises a peristaltic pump 3 and stock solution 12, an inlet of the peristaltic pump 3 is communicated with a two-way joint 1, an outlet of the peristaltic pump 3 is communicated with a three-way joint 5, two outlets of the three-way joint 5 are both communicated with pinch valves 2, outlets of the two pinch valves 2 are both communicated with two-way joints 1, an outlet of one two-way joint 1 is communicated with an inlet of the stock solution 12, one side of the stock solution 12 is provided with a filtering membrane 6, an outlet of the stock solution 12 is communicated with two-way joints 1, three of the five two-way joints 1 are sequentially communicated with a filtering module 10, a cleaning solution bottle and a waste solution bottle at the last stage, and the other two-way joints 1 are in butt joint, the filtering module 10 comprises a peristaltic pump 3, a filtering membrane 6, a transfer bottle 8 and a hollow fiber column 4, an inlet of the peristaltic pump 3 is communicated with two-way joints 1, and the two-way joints 1 are communicated with the hollow fiber column 4, two outlets of the hollow fiber column 4 are both communicated with a pinch valve 2, the outlet of one of the two pinch valves 2 is communicated with a three-way joint 5, one outlet of the three-way joint 5 is communicated with the pinch valve 2, the other outlet of the three-way joint 5 is communicated with a transfer bottle 8, the outlet of the pinch valve 2 is communicated with the three-way joint 5, one outlet of the three-way joint 5 is communicated with a two-way joint 1, the other outlet of the three-way joint 5 is communicated with the pinch valve 2, and one side of the transfer bottle 8 is provided with a filter membrane 6;
two of the six two-way joints 1 are connected to a host module 11 or a filtering module 10 at the previous stage, one two-way joint 1 close to the hollow fiber column 4 is connected to a waste liquid bottle, one two-way joint 1 close to the transfer bottle 8 and the two-way joint 1 on the three-way joint 5 far away from the transfer bottle 8 are communicated with the filtering module 10 at the next stage, and the other two-way joints 1 are in butt joint;
the harvesting module 9 comprises two peristaltic pumps 3, two filtering membranes 6 and a hollow fiber column 4, wherein the outlet of one of the two peristaltic pumps 3 is communicated with the hollow fiber column 4, the inlet of the other peristaltic pump 3 is communicated with the hollow fiber column 4, the outlet of the other peristaltic pump 3 is communicated with the harvesting bottle 7, the inlet of the peristaltic pump 3 at the inlet of the hollow fiber column 4 is communicated with a two-way joint 1, the two-way joint 1 is communicated with a last-stage filtering module 10, the outlet of the hollow fiber column 4 is communicated with a three-way joint 5, the inlet of the three-way joint 5 is communicated with the two-way joint 1 through a pinch valve 2, the two-way joint 1 is communicated with cleaning liquid, the filtering membranes 6 are arranged at the other outlet of the three-way joint 5 and one side of the harvesting bottle 7, the outlet of the hollow fiber column 4 far away from the three-way joint 5 is communicated with the two-way joint 1 through the pinch valve 2, and the two-way joint 1 is communicated with the waste liquid bottle.
The invention adopts the current more advanced hollow fiber column 4, breaks the limitation of single or fixed filtration stage number, and is suitable for various occasions with purification or concentration requirements, wherein, a filtration module 10 and an acquisition module are added to a host module 11, when multistage filtration is required, the scheme can be set as that the host module 11 and a plurality of filtration modules 10 and the acquisition modules are added, wherein, a plurality of filtration modules 10 can adopt the mode of random combination of series connection, parallel connection or series-parallel connection to achieve the effects required by different application occasions
Example two
Referring to fig. 1-5, the method of using the automated concentration system with scalable multi-stage filtration comprises the following steps:
the method comprises the following steps: the host module 11 pumps the cleaning solution into the stock solution 12, and the filtering module 10 pumps the stock solution 12 mixed with the cleaning solution into the transfer bottle 8 through the hollow fiber column 4 in the filtering module 10 to obtain a filtrate;
step two: the harvesting module 9 pumps the filtrate in the transfer bottle 8 into the hollow fiber column 4 in the harvesting module 9, the concentrated solution is left in the hollow fiber column 4, and the waste liquid is discharged to a waste liquid bottle;
step three: the harvesting module 9 pumps the concentrated solution in the hollow fiber column 4 back to the harvesting bottle 7.
EXAMPLE III
Referring to fig. 1 to 5, when a plurality of filter modules 10 are provided and all communicate with a host module 11, they are filtered one by one in the above-mentioned first step, and when a plurality of filter modules 10 are connected in series, they are filtered by hollow fiber columns 4 in the filter modules 10, and finally pumped into a harvesting module 9 through the above-mentioned second step.
Example four
Referring to fig. 1-5, the concentration process is: opening a pinch valve 2 and a peristaltic pump 3 on a host module 11, pumping a cleaning solution into a stock solution 12, closing the pinch valve 2 and the peristaltic pump 3 on the host module 11, opening the pinch valve 2 and the peristaltic pump 3 on a filter module 10, opening the pinch valve 2 and the peristaltic pump 3 on a harvesting module 9, then closing the pinch valve 2 and the peristaltic pump 3 on the filter module 10 and the harvesting module 9, performing cell concentration and filtration, and opening the other peristaltic pump 3 on the harvesting module 9 for harvesting.
EXAMPLE five
Referring to fig. 1-5, the cleaning process is: opening a pinch valve 2 and a peristaltic pump 3 for cleaning on a host module 11, opening the pinch valve 2 for communicating a filter module 10 with the host module 11, starting the peristaltic pump 3 on the filter module 10, pumping a cleaning solution into the filter module 10, then closing the peristaltic pump 3 on the host module 11 and the filter module 10, closing the pinch valve 2, cleaning, then opening the peristaltic pump 3 on the filter module 10, and discharging the cleaning solution.
The foregoing shows and describes the general principles, principal features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. An automatic concentration system capable of expanding multistage filtration and a using method thereof comprise a host module (11), a filtration module (10) and a harvesting module (9), and are characterized in that the filtration module (10) is provided with a plurality of filtration modules, wherein the filtration modules (10) can be connected in series or in parallel;
the filtering modules (10) are connected in series, and the operation steps comprise the following points:
the method comprises the following steps: the host module (11) is communicated with one of the plurality of filtering modules (10), two adjacent filtering modules (10) are communicated with each other, and one filtering module (10) far away from the host module (11) is communicated with the harvesting module (9);
step two: the method comprises the following steps of (1) communicating a host module (11) with stock solution (12), communicating cleaning solution with the host module (11), pumping the cleaning solution into the stock solution (12), communicating the stock solution (12) with a filtering module (10), and communicating a transfer bottle (8) with one side of the filtering module (10) far away from one of the host modules (11);
step three: the outlet of the transfer bottle (8) is communicated with the harvesting module (9), and the outlet of the harvesting module (9) is communicated with a harvesting bottle (7);
step four: an outlet at one end of the cleaning liquid equipment is communicated with the harvesting module (9).
When the filtering modules (10) are connected in parallel, all the filtering modules (10) are communicated with the host module (11) and the stock solution (12).
2. The automatic concentration system of expandable multi-stage filtration according to claim 1, wherein the host module (11) comprises a peristaltic pump (3) and a stock solution (12), the inlet of the peristaltic pump (3) is communicated with a two-way joint (1), the outlet of the peristaltic pump (3) is communicated with a three-way joint (5), two outlets of the three-way joint (5) are communicated with pinch valves (2), the outlets of the two pinch valves (2) are communicated with two-way joints (1), the outlet of one two-way joint (1) is communicated with the inlet of the stock solution (12), one side of the stock solution (12) is provided with a filter membrane (6), and the outlet of the stock solution (12) is communicated with the two-way joint (1);
three of the five two-way joints (1) are sequentially in butt joint with the last stage of filtering module (10), the cleaning solution and the waste liquid bottle, and the other two-way joints (1).
3. The automatic concentration system of the expandable multistage filtration according to claim 1, wherein the filtration module (10) comprises a peristaltic pump (3), a filtration membrane (6), a transfer bottle (8) and a hollow fiber column (4), an inlet of the peristaltic pump (3) is communicated with a two-way joint (1), the two-way joint (1) is communicated with the hollow fiber column (4), two outlets of the hollow fiber column (4) are communicated with pinch valves (2), an outlet of one of the two pinch valves (2) is communicated with a three-way joint (5), one outlet of the three-way joint (5) is communicated with the pinch valve (2), the other outlet of the three-way joint (5) is communicated with the transfer bottle (8), wherein the outlet of the pinch valve (2) is communicated with the three-way joint (5), one outlet of the three-way joint (5) is communicated with the outlet of the two-way joint (1), the other outlet of the three-way joint (5) is communicated with a pinch valve (2), and one side of the transit bottle (8) is provided with a filtering membrane (6);
two of the six two-way joints (1) are connected to a host module (11) or a previous-stage filtering module (10), one two-way joint (1) close to the hollow fiber column (4) is connected to a waste liquid bottle, one two-way joint (1) close to the transfer bottle (8) and the two-way joint (1) on the three-way joint (5) far away from the transfer bottle (8) are communicated with the next-stage filtering module (10), and the other two-way joints (1) are in butt joint.
4. The automated concentration system of expandable multi-stage filtration according to claim 1, wherein the harvest module (9) comprises two peristaltic pumps (3), two filtration membranes (6), one hollow fiber column (4), the outlet of one of the two peristaltic pumps (3) is communicated with the hollow fiber column (4), the inlet of the other peristaltic pump (3) is communicated with the hollow fiber column (4), the outlet of the other peristaltic pump (3) is communicated with the harvest bottle (7), the inlet of the peristaltic pump (3) at the inlet of the hollow fiber column (4) is communicated with the two-way joint (1), the two-way joint (1) is communicated with the last stage filtration module (10), the outlet of the hollow fiber column (4) is communicated with the three-way joint (5), and the inlet of the three-way joint (5) is communicated with the two-way joint (1) through the pinch valve (2), and the two-way joint (1) is communicated with the cleaning solution, a filtering membrane (6) is arranged at the other outlet of the three-way joint (5) and one side of the harvesting bottle (7), the outlet of the hollow fiber column (4) far away from the three-way joint (5) is communicated with the two-way joint (1) through a pinch valve (2), and the two-way joint (1) is communicated with the waste liquid bottle.
5. The method for using the scalable multi-stage filtration automatic concentration system according to claim 1, wherein the method for using the scalable multi-stage filtration automatic concentration system comprises the following steps:
the method comprises the following steps: the host module (11) pumps cleaning fluid into stock solution (12), the filtering module (10) pumps the stock solution (12) mixed with the cleaning fluid into the transfer bottle (8) through the hollow fiber column (4) in the filtering module (10) to obtain filtrate;
step two: the harvesting module (9) pumps the filtrate in the transfer bottle (8) into the hollow fiber column (4) in the harvesting module (9), the concentrated solution is left in the hollow fiber column (4), and the waste liquid is discharged to a waste liquid bottle;
step three: the harvesting module (9) pumps the concentrated solution in the hollow fiber column (4) back to the harvesting bottle (7).
6. The method of claim 5, wherein the filtration modules (10) are arranged in plurality and all communicate with the host module (11), and when a plurality of filtration modules (10) are connected in series, the filtration modules are filtered through the hollow fiber columns (4) in the filtration modules (10) and then pumped into the harvesting module (9) through the second step.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210535631.8A CN114768373A (en) | 2022-05-17 | 2022-05-17 | Automatic concentration system capable of expanding multistage filtration and use method thereof |
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| CN202210535631.8A CN114768373A (en) | 2022-05-17 | 2022-05-17 | Automatic concentration system capable of expanding multistage filtration and use method thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102086448A (en) * | 2010-12-13 | 2011-06-08 | 南宁庞博生物工程有限公司 | Biological enzyme liquid/beverage multistage ultrafiltering-purifying-separating-concentrating device |
| JP2015126763A (en) * | 2013-12-27 | 2015-07-09 | 旭化成メディカル株式会社 | Ascites filtration concentration system, method for cleaning filter and concentrator in ascites filtration concentration system, and method for recovering filtrate and concentrated liquid in cleaning filter and concentrator |
| CN106914137A (en) * | 2015-12-24 | 2017-07-04 | 国家开发投资公司 | A kind of film concentration systems and method |
| CN112251333A (en) * | 2020-10-12 | 2021-01-22 | 北京诺德观呈医疗科技有限公司 | Device and method for purifying cell exosomes |
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2022
- 2022-05-17 CN CN202210535631.8A patent/CN114768373A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102086448A (en) * | 2010-12-13 | 2011-06-08 | 南宁庞博生物工程有限公司 | Biological enzyme liquid/beverage multistage ultrafiltering-purifying-separating-concentrating device |
| JP2015126763A (en) * | 2013-12-27 | 2015-07-09 | 旭化成メディカル株式会社 | Ascites filtration concentration system, method for cleaning filter and concentrator in ascites filtration concentration system, and method for recovering filtrate and concentrated liquid in cleaning filter and concentrator |
| CN106914137A (en) * | 2015-12-24 | 2017-07-04 | 国家开发投资公司 | A kind of film concentration systems and method |
| CN112251333A (en) * | 2020-10-12 | 2021-01-22 | 北京诺德观呈医疗科技有限公司 | Device and method for purifying cell exosomes |
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